ライフサイエンスコーパス: spin label

1 uces >90% helicity and is unperturbed by the spin label.

2 n those found with the commonly used protein spin label.

3 e spectroscopic properties of fluorescent or spin label.

4 tances between conformationally well-defined spin labels.

5 de, MARCKS-ED, to membranes with and without spin labels.

6 ould be individually modified with nitroxide spin labels.

7 mical exchange processes involving nitroxide spin labels.

8 e LHCII trimers in which only one monomer is spin-labeled.

9 s on T4 lysozyme introduced by site-directed spin labeling.

10 cerebral perfusion was examined by arterial spin labeling.

11 images and to perfusion images from arterial spin labeling.

12 gional perfusion was measured using arterial spin labelling.

13 flow (rCBF) using pseudo-continuous arterial spin labelling.

14 2 fibrils show stronger interactions between spin labels across the full range of the Abeta42 sequenc

15 In contrast, both the proteins and the spin label alone, when in a glycerol-water mixture below

16 port the genetic encoding of a noncanonical, spin-labeled amino acid in Escherichia coli.

17 EPR of unphosphorylated Noxa, with spin-labeled amino acid TOAC incorporated within the BH3

18 ral perturbations by the bulkier diamagnetic spin label analog.

19 gned seven variants of GB1 domain bearing R1 spin label and recorded the corresponding MD trajectorie

20 Here, we use site-directed spin labeling and a novel total internal reflection fluo

21 Arterial spin labeling and asymmetric spin echo sequences measure

22 We have quantified both site-directed spin labeling and dehydroalanine formation.

23 Here we report a systematic spin labeling and double electron electron resonance (DE

24 the cytoplasmic surface using site-directed spin labeling and double electron-electron resonance spe

25 hyl-based labels, approach for site-directed spin labeling and efficient immobilization procedure tha

26 come this problem by utilizing site-directed spin labeling and electron paramagnetic resonance (EPR)

27 e distance data gathered using site-directed spin labeling and electron paramagnetic resonance spectr

28 This model is supported by site-directed spin labeling and electron paramagnetic resonance spectr

29 e assessed using voxel-based pulsed arterial spin labeling and morphometric analyses and tested for a

30 dy support the possibility of using arterial spin labeling and pattern analysis of dynamic susceptibi

31 Arterial spin labeling and seed-based resting state functional co

32 Participants underwent two arterial spin labeling and two blood oxygen level-dependent scans

33 Using site-directed spin labelling and electron paramagnetic resonance spect

34 lly distinguishable nitroxide and gadolinium spin labels and Double Electron-Electron Resonance can h

35 Previous spin-labeling and fluorescence resonance energy transfer

36 Here we employ spin-labeling and pressure-resolved double electron-elec

37 ns in GPCR catalytic function; 2) the use of spin-labeling and variable-pressure electron paramagneti

38 embrane vitamin B(12) transporter, BtuB, was spin-labeled, and double electron-electron resonance was

39 eine mutants in a soluble CNBD fragment were spin-labeled, and interspin label distance distributions

40 -functionalized gold nanoparticles through a spin label are presented.

41 Our simulations show that the depths of spin labels are approximately 6-17 A deeper than the unl

42 Nitroxide spin labels are used for double electron-electron resona

43 functionalities onto proteins and attached a spin label as close as possible to the protein backbone,

44 and T(1) and T(2) relaxation times make them spin labels as good as the benchmark FTR.

45 , this work establishes 2'-alkynyl nitroxide spin-labelling as a minimally perturbing method for prob

46 Purpose To determine if arterial spin labeled (ASL) MRI perfusion changes are associated

47 Purpose To compare arterial spin labeling (ASL) data between low- and high-grade bra

48 Arterial spin labeling (ASL) is a magnetic resonance (MR) imaging

49 Arterial spin labeling (ASL) is a neuroimaging technique used to

50 collateral vessels identified using arterial spin labeling (ASL) magnetic resonance imaging, a techni

51 Here, we investigated arterial spin labeling (ASL) perfusion CMR as a novel approach to

52 ate pattern recognition analysis of arterial spin labeling (ASL) perfusion maps can be used for class

53 diffusion tensor imaging (DTI) and arterial spin labeling (ASL) to discriminate patients with early

54 graphy, carotid plaque imaging, and arterial spin labeling (ASL) to identify imaging parameters that

55 el-encoded multi-postlabeling delay arterial spin labeling (ASL) was used to separately quantify the

56 ical magnetic resonance scans using arterial spin labeling (ASL) were performed to study the haemodyn

57 Arterial spin labeling (ASL), as a non-invasive and non-contrast

58 plementary neuroimaging techniques: arterial spin labeling (ASL), blood oxygen level-dependent (BOLD)

59 nsor imaging (DTI) acquisitions and arterial spin labeling (ASL).

60 Using arterial spin labelled (ASL) magnetic resonance imaging, this is

61 CBF) alterations in IGE detected by arterial spin labelling (ASL) perfusion magnetic resonance imagin

62 In analyses of arterial spin-labeled (ASL) MRI, nonresponders exhibited increase

63 EPR) approaches: the rotational diffusion of spin labels at 55 residues with continuous-wave EPR, and

64 copy, we observed that the distances between spin labels at positions 311 and 328 in the fibril core

65 decaribonucleotide derivative with nitroxide spin labels at terminal nucleotides was utilized.

66 ron paramagnetic resonance spectroscopy with spin-labeled ATP analogs to probe the structure of the A

67 and diffusion dynamics in the vicinity of a spin label attached to a cysteine in the Tyr71 --> Cys G

68 an alternative sequence in which a nitroxide spin label attached to cysteine has been introduced at i

69 of intra- and intersubunit distances between spin labels attached to surface-engineered cysteines.

70 nonaribonucleotide pUUCGUAAAA with nitroxide spin labels attached to the 5'-phosphate and to the C8 a

71 by exploring the distance histograms between spin-labels attached to T4 lysozyme.

72 of brain activity using continuous arterial spin labeling based functional magnetic resonance imagin

73 synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points

74 eins, synthesizing the necessary quantity of spin-labeled biomolecules (typically 50 pmol to 100 pmol

75 idual magnetic molecules, nanostructures and spin-labelled biomolecules.

76 tion proceeded efficiently with fluorescent, spin-labeled, biotinylated, or cross-linker-modified gua

77 a multiband multi-echo simultaneous arterial spin labelling/blood oxygenation level dependent (ASL/BO

78 rotonated fatty acid and phosphatidylcholine spin labels, both of which have a considerably lower aff

79 ramagnetic resonance (EPR) of a bifunctional spin label (BSL) bound stereospecifically to Dictyosteli

80 ron paramagnetic resonance of a bifunctional spin label (BSL) to build and refine atomistic models of

81 tice relaxation rate (T1(-1)) of a nitroxide spin label by a paramagnetic metal.

82 R spectra of GB1 domain with solvent-exposed spin label can be accurately reproduced by means of Redf

83 l analysis indicated that orientation of the spin label can be determined within <2.1 degrees accurac

84 ombining double-histidine motifs with Cu(II) spin labels can further increase the precision of distan

85 The required spin-labeled chain ends were introduced efficiently via

86 phyrin triplet state (S = 1) and a nitroxide spin label chemically incorporated into a small helical

87 s and interspin distances were measured to a spin-labeled cobalamin using pulse EPR spectroscopy.

88 However, the spin labels commonly employed are highly flexible, which

89 the ones reported by the more standard MTSSL spin label, commonly employed in protein studies.

90 pin resonance spectroscopy and site-specific spin-labeling confirm that the Tsr HAMP maintains a four

91 s are considered rigid; the position of each spin-label conformer and the structure of each protein c

92 optimizing the positions and populations of spin-label conformers against intradomain paramagnetic r

93 We used circular dichroism and site-directed spin labeling coupled with electron paramagnetic resonan

94 es to calculate their distances to a trio of spin-labeled Cys mutants.

95 one should be cautious in interpretation of spin label data when charged and polar residues in small

96 ophobic residue Phe is labeled, however, the spin-label depth is close to that of the native residue

97 for this regulation, wild-type RyRp and four spin-labeled derivatives were synthesized, each containi

98 We used PC spin labels dipalmitoylphospatidyl-tempo-choline (on the

99 flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides a

100 troscopy, X-ray crystal structures of B-form spin-labelled DNA duplexes, molecular dynamics simulatio

101 t computational comparison, we find that the spin label does not perturb the signature population of

102 Spin-labeled double-cysteine mutants of VcSiaP were anal

103 Here, we combine spin-labeling double electron-electron resonance (DEER)

104 nstraints (e.g., engineered metal bridges or spin-labels), each treated as an individual molecular fr

105 Here, we report the synthesis of two new spin labels, EC and ECm, both of which possess the rigid

106 the C-terminus of EcMscL using site-directed spin labelling electron paramagnetic resonance (SDSL EPR

107 system, which we confirmed by site-directed spin-label electron paramagnetic resonance spectroscopy.

108 In this study, we use site-directed spin-labeling electron paramagnetic resonance spectrosco

109 Site-directed spin-labeling electron paramagnetic resonance spectrosco

110 In this study, we used site-directed spin-labeling electron paramagnetic resonance spectrosco

111 addition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron r

112 First, the spin-label ensemble is determined by optimizing the posi

113 ack reactive cysteines and that paramagnetic spin labels entering the periplasm are selectively reduc

114 Site directed spin labeling EPR and DEER (double electron-electron res

115 h STAM1 activates FAK, we used site-directed spin-labeling EPR spectroscopy-based studies coupled wit

116 e imaging, dynamic nuclear polarization, and spin-labeling EPR under in-cell conditions.

117 These findings, in combination with spin-labeling/EPR spectroscopic measurements in reconsti

118 amate (Glu) and glutamine (Gln) and arterial spin labeling evaluation for rCBF.

119 NMR and spin-labeling experiments showed that GH5_pMut bound to

120 ay traces and distance distributions between spin labels fast enough to fold proteins de novo.

121 ace are targeted by using negatively charged spin-labeled fatty acids that display selectivity of int

122 t there exist a number of challenges such as spin-label flexibility, domain dynamics, and overfitting

123 dient-recalled echo to assess CMBs, arterial spin labeling for CBF, and T1- and T2-weighted imaging f

124 tial of double-histidine (dHis)-based Cu(II) spin labeling for the identification of various conforma

125 which serve as models in the search for new spin labels for DEER distance measurement at room temper

126 n healthy individuals (n=23) during arterial spin labeling functional magnetic resonance imaging (fMR

127 Pseudocontinuous arterial spin labeling functional magnetic resonance imaging and

128 Using arterial spin labeling functional magnetic resonance imaging, we

129 Arterial spin-labeled functional magnetic resonance imaging track

130 Using arterial spin-labeled functional magnetic resonance imaging, we m

131 tivity, which was assessed by using arterial spin-labeling functional magnetic resonance imaging 4 h

132 and disease parameters, we used an arterial-spin-labeling functional MRI stress paradigm in 36 MS pa

133 Here, we demonstrate that the Gd(III) based spin label Gd-PyMTA is suitable for in-cell EPR.

134 erimentally derived distance measurements of spin-labeled GLIC suggest ELIC is not a good model for t

135 a at each state of the refolding workflow of spin-labeled Haloarcula marismortui bacteriorhodopsin-I

136 A spirocyclohexyl spin label has been prepared that has longer Tm between

137 resonance in conjunction with site-directed spin labeling has been used to probe natural conformatio

138 ce (DEER), in conjunction with site-directed spin-labeling, has emerged in the past decade as a power

139 n extensive toolkit of EPR methods, multiple spin labels have been developed and utilized, among them

140 Arterial spin labelling identified no significant changes in regi

141 : (i) reduction resistant Gd(3+) chelates as spin labels, (ii) high frequency (94.9 GHz) for sensitiv

142 Pseudo-continuous arterial spin labeling imaging was used to measure resting region

143 clear magnetic resonance, combining arterial spin-labeling imaging of perfusion, and (31)P-spectrosco

144 ent of 140 amol of the most common nitroxide spin label in a approximately 593 fL liquid cell at ambi

145 ation process for a pH-sensitive imidazoline spin label in aqueous solution where the exchange rate a

146 S) of Escherichia coli, mutants containing a spin label in the cytosolic or the transmembrane region

147 with the assumption that the position of the spin label in the membrane is close to that of the nativ

148 Site-directed spin labeling in combination with double electron-electr

149 Site-directed spin labeling in combination with EPR is a powerful meth

150 stituted synaptotagmin 1 using site-directed spin labeling in which we characterize the linker region

151 uantum bits, and determining the location of spin labels in biological systems.

152 n-electron resonance (DEER) between pairs of spin labels in MdfA, reconstituted in nanodiscs, with cy

153 the instabilities of the standard nitroxide spin labels in the cell environment and the limited sens

154 hort lifetime of the commonly used nitroxide spin labels in the reducing milieu inside a cell.

155 lamin transporter BtuB was overexpressed and spin-labeled in whole cells and outer membranes and inte

156 collagen V mimic (synthesized with nitroxide spin labels) in the active site of the catalytic domain

157 ng approach, employing nitroxide and Gd(III) spin labels, in conjunction with Q-band and W-band doubl

158 ctions could be detected using site-directed spin labels, indicating that the three helices do not ad

159 EPR showed that a spin label inserted near the N-terminus was weakly immob

160 Residue-level mobility analysis on spin labels introduced at 14 different positions shows a

161 Nitroxide spin labels, introduced specifically at three individual

162 The spin label is assembled in situ from natural amino acid

163 copy (EPR) in combination with site-directed spin labeling is a very powerful tool to monitor the str

164 lowing overexpression of the target protein, spin labeling is performed with E. coli or isolated oute

165 uble resonance (PELDOR), using site-directed spin labeling, is most commonly employed to accurately d

166 EPR spectra were obtained for the spin-labeled ligands both free in solution and attached

167 Here, librational motion of spin-labeled lipid chains in membranous Na,K-ATPase is i

168 In this work spin-labeled lipid molecules (SL-lipids), when used as p

169 We acquired pulsed arterial spin labeling magnetic resonance imaging data in 44 gene

170 Arterial spin labeling magnetic resonance imaging was used to col

171 Arterial spin labelling magnetic resonance imaging recognized reg

172 an older adults (n = 232) underwent arterial spin labelling magnetic resonance imaging to measure reg

173 ced cerebral blood flow measured by arterial spin labelling magnetic resonance imaging, but it is unc

174 halopathy lesions was determined by arterial spin labelling magnetic resonance imaging.

175 antified on a voxelwise basis using arterial spin-labeled magnetic resonance imaging at 3T.

176 pposing sides of the catalytic domain engage spin-labelled membrane mimics.

177 onventional line shapes, similar to multiply spin-labeled membranous Na,K-ATPase below 200 K.

178 In multiply spin-labeled membranous Na,K-ATPase, this heterogeneous

179 Using the site-directed spin labeling method of electron paramagnetic resonance

180 al and (15)N relaxation data, NMR data using spin-labeled micelles, and MD simulations of micelle-ass

181 in helical dynamics we observed for ensemble spin-label mobility reflected differences in proportions

182 We measured distances between pairs of spin labels monitoring the movement of the nucleotide bi

183 philic archaeon Pyrococcus furiosus Pairs of spin labels monitoring the two sides of the transporter

184 Guided by these parameters, an arterial spin labeling MR imaging approach was adapted to measure

185 922 youths ages 8-22 y imaged using arterial spin labeled MRI as part of the Philadelphia Neurodevelo

186 These results suggest that arterial spin labelling MRI may be a promising non-invasive imagi

187 3T arterial spin labelling MRI scans from 162 participants in the PR

188 ated cross-sectional differences in arterial spin labelling MRI-based cerebral blood flow between pre

189 We then used arterial spin-labeling MRI to noninvasively measure CBF and asses

190 neered cysteines with a methanethiosulfonate spin label (MTSL) with minimal background signals.

191 mation is refined by attaching two different spin labels, MTSL or BSL (bifunctional spin-label) onto

192 Intermolecular distances on four singly spin-labeled mVDAC1 mutants were used to generate a mode

193 Spin labeling nucleic acids at specific sites requires t

194 Using blebbistatin we show that spin-labeled nucleotides bound to myosin have an oriente

195 on-electron double resonance measurements of spin-labeled OAM were used to provide direct evidence fo

196 ce tools that rely on site-specific electron spin labeling of Deltatau187.

197 f monocysteine variants and by site-specific spin labeling of the Q-helix followed by EPR-based inter

198 ere studied using pseudo-continuous Arterial Spin Labelling on 2 occasions, once after 40IU intranasa

199 upportive, and pinpoint the locations of the spin labels on the duplexes.

200 We used phosphocholine spin labels on the lipid headgroup and different positio

201 cal and beta-sheet aqueous proteins that are spin-labeled on a single cysteine residue display spin-e

202 Current distance measurements between spin-labels on multimeric protonated proteins using doub

203 erent spin labels, MTSL or BSL (bifunctional spin-label) onto the F or G helices and using DEER (doub

204 Comparison of distance distribution of spin label pairs on the periplasm with those calculated

205 subsequent protein expression, OM isolation, spin labeling, PELDOR experiment, and data analysis typi

206 The results suggest that the spin labeled peptide H-AP10C(Gd-PyMTA)P10C(Gd-PyMTA)P10-

207 The EPR spectrum of each spin-labeled peptide indicates nanosecond dynamic disord

208 Electron spin resonance on spin-labeled peptides confirms these observations.

209 antified on a voxelwise basis using arterial spin labeled perfusion MRI at 3T.

210 Conclusion Arterial spin labeled perfusion MRI may assist in identifying res

211 Here, we combined arterial spin labeling perfusion and blood oxygen level-dependent

212 ndividuals with schizophrenia using arterial spin labeling perfusion MRI.

213 hunting ranging from (37-60%) using arterial spin labeling perfusion.

214 3 T), diffusion tensor imaging, and arterial spin labelled perfusion imaging.

215 owing to local environmental effects on the spin-label phase memory relaxation time Tm .

216 ects and substantially increase the apparent spin-label phase memory relaxation time, complemented by

217 shows that rotational motion of a nitroxide spin label, placed at the N-terminal end of the first be

218 f-assembled nanoribbon with radical electron spin labels positioned at known distances off the surfac

219 oscillations observed for most of the double spin-labeled positions indicate a rather rigid orientati

220 and derivatives thereof using site-directed spin labeling, pressure-resolved double electron-electro

221 Moreover, the signal of spin-labeled protein can be selectively detected in cell

222 ein interface strongly resembles that of the spin-labeled protein side chains, suggesting solvent-med

223 , we compared the stability of the different spin-labeled protein variants in E. coli and HeLa cell e

224 igh resolution field cycling (31)P NMR using spin-labeled protein) are combined with enzyme kinetics

225 ation of electron paramagnetic resonance, on spin-labeled protein, and disulfide crosslinking, we sho

226 irwise P(r) distance distributions in doubly spin labeled proteins.

227 is enables the intracellular biosynthesis of spin-labeled proteins and obviates the need for any chem

228 Continuous wave-ESR of the spin-labeled proteins confirms that broader PDS distance

229 a method was developed for rapidly freezing spin-labeled proteins under pressure to kinetically trap

230 in vivo imaging to distance measurements in spin-labelled proteins.

231 e distance and angle measurements with rigid spin labels provide critical input for the refinement of

232 EER) experiments of nucleic acids with rigid spin labels provide highly accurate distance and orienta

233 three-dimensional pulsed-continuous arterial spin labeling provided measurements of regional cerebral

234 netic resonance imaging with pulsed arterial spin labelling provided serial measurements of global CB

235 as induced by a protein-attached lanthanide spin label, provided structural restraints for the prote

236 The structure of the spin-labeled Q54R1/L173R1 R125A mutant was solved at 2.1

237 We exploited arterial spin-labeling quantitative perfusion imaging and a newly

238 Moreover, NMR and spin-labeling results from the study of the nucleosome i

239 NMR experiments using paramagnetic spin labels reveal how SspH1 binds Ube2D~Ub and targets

240 electron-electron resonance measurements of spin-labeled rhodopsin.

241 et of 112 participants who received arterial spin labelling scans, faster aortic stiffening was also

242 using circular dichroism (CD), Site-Directed Spin Labeling (SDSL) coupled to EPR spectroscopy, and en

243 Site-directed spin labeling (SDSL) ESR is a valuable tool to probe pro

244 osecond backbone dynamics with site-directed spin labeling (SDSL) in soluble proteins has been well e

245 In the present study, site-directed spin labeling (SDSL) together with double electron-elect

246 PR) spectroscopy, coupled with site-directed spin labeling (SDSL), is a useful method for studying co

247 With an arterial spin labeling sequence, three networks were first identi

248 t room temperature and EPR spectroscopy on a spin-labeled single crystal allows to correlate the stru

249 ed to guide positioning of a small number of spin-labeled single-Cys mutants that coat the entire enz

250 or resonances more than 20 residues from the spin-labeling site.

251 fer by concerted changes in the positions of spin-label sites at the base of the bundle.

252 d protein transitions, the ability of single spin-label sites to detect conformational heterogeneity,

253 ctive insights into these processes, but new spin-labeling strategies are needed.

254 tracellular loop and partially dissociates a spin-labeled substrate analog.

255 The method relies on sparse spin-labeling, supplemented by deuteration of protein an

256 D2O is determined for stearic acid, n-SASL, spin-labeled systematically at the C-n atoms throughout

257 tly homogeneous broadening are found in both spin-label systems.

258 the method for a model system as well as for spin-labeled T4 lysozyme in explicit water, and show how

259 romises between coarse-grained models of the spin label that lower the resolution and explicit models

260 a tonic pain model with concurrent arterial spin labelling that measures cerebral perfusion related

261 uous wave EPR spectroscopy and site-specific spin labels that directly probed, in essentially physiol

262 To employ in-cell EPR using nitroxide-based spin labels, the structure of the nitroxides must confer

263 e-matched healthy controls, we used arterial spin labeling to assess the effects of kidney transplant

264 We generated seven mutants suitable for spin labeling to enable application of pulsed EPR techni

265 e imaging based on pseudocontinuous arterial spin labeling to measure CBF at normocapnia (ie, breathi

266 a placebo-controlled study, we used arterial spin labeling to measure IN-OT-induced changes in restin

267 gnetic resonance imaging technique, arterial spin labelling to measure perfusion.

268 interest (doping/attachment of paramagnetic spin labels to biomolecules of interest).

269 used distance measurements between pairs of spin labels to define the conformational cycle of the Na

270 ite-specifically attached pairs of nitroxide spin labels to monitor changes in the mini TAR DNA stem-

271 We attach nitroxide radicals (i.e., spin labels) to multiple phosphate backbone positions of

272 -binding site of SULT1A1-is determined using spin-label triangulation NMR.

273 tructure of the new site is determined using spin-label-triangulation NMR.

274 Here we pioneer the use of their spin-labeled variants as reporters of conformational dyn

275 in, as an alternative to the introduction of spin labels via engineered cysteine residues.

276 Conditions for attachment of the spin-label via esterification have been optimized on the

277 performed using velocity-selective arterial spin labeling (VSASL) and 3D image acquisition with whol

278 When spin-labeled WALP24 was inserted in two representative l

279 The paramagnetic nitroxide spin label was attached to Cys residues that were placed

280 thod based on the technique of site-directed spin labeling was developed to experimentally map shapes

281 Here, site-directed spin labeling was used to probe the solution structures

282 Arterial spin labelling was used to index resting-state perfusion

283 Using site-directed spin labeling, we demonstrated that the pressure- and te

284 Using arterial spin labeling, we measured resting-state cerebral blood

285 ectroscopy in combination with site-directed spin labeling, we show that familial PD-associated varia

286 mages and perfusion images by using arterial spin labeling were obtained for comparison.

287 Pairs of spin labels were introduced at residues selected to trac

288 To detect conformational changes, pairs of spin labels were introduced into arrestin-2 and arrestin

289 ial CNG channel from Spirochaeta thermophila Spin labels were introduced into the SthK C-linker, a do

290 The distances between attached spin labels were measured using pulsed electron-electron

291 antiparallel-arranged dimer structures when spin labels were placed into the PCM region.

292 An R5a nitroxide spin label, which was covalently attached at a specific

293 ic sites requires the covalent attachment of spin labels, which involves rather complicated and labor

294 MRI arterial spin labeling, white matter hyperintensities (WMHs) and

295 ptide H-AP10CP10CP10-NH2 was site-directedly spin labeled with Gd-PyMTA at both cysteine moieties.

296 ance is extended to approximately 40 A using spin labels with long T1, a high-affinity 5-residue Cu(2

297 paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitiv

298 omogeneity and investigated by site-directed spin-labeling with pulse-dipolar electron-spin resonance

299 nance (EPR) spectroscopy of oligonucleotides spin-labelled with triazole-appended nitroxides at the 2

300 n topology based on the accessibility of the spin label, with the assumption that the position of the