ライフサイエンスコーパス: spindle

1 ging from contractile bundles to the mitotic spindle.

2 microtubule-stabilizing protein GTSE1 to the spindle.

3 a process that specifically off-centers the spindle.

4 pindle assembly but is toxic for the mitotic spindle.

5 fluence between membranes, chromatin and the spindle.

6 the formation and maintenance of the bipolar spindle.

7 toskeleton and form the poles of the mitotic spindle.

8 inetochore-fibers connect chromosomes to the spindle.

9 astral microtubules to position the mitotic spindle.

10 les RanGTP to target endogenous XCTK2 to the spindle.

11 subpellicular microtubules, and the mitotic spindle.

12 namic microtubules, comprising the metaphase spindle.

13 can act on astral microtubules to orient the spindle.

14 xplain how microtubules are generated in the spindle.

15 MN2) orchestrates the initial movement of MI spindle.

16 s/centrosomes and the maintenance of bipolar spindles.

17 als mechanical conditions for these abnormal spindles.

18 ncreased pairwise correlations during nested spindles.

19 chromosomes are properly attached to mitotic spindles.

20 with the inter-filament spacing in metaphase spindles.

21 produce regulated mechanical outputs within spindles.

22 Inhibition of monopolar spindle 1 (MPS1) kinase represents a novel approach to c

23 some, a structure that assembles the mitotic spindle [1], is notably large in the zebrafish embryo (2

24 nables chromosome segregation by the mitotic spindle(1).

25 mosomes in mitosis, cells assemble a mitotic spindle, a molecular machine with centrosomes at two opp

26 n-regulates kindlin-2 expression, leading to spindle abnormalities not only in the SH-SY5Y cell line,

27 ate dynamics distinguished by sustained high spindle activities.

28 Schizophrenia is characterized by reduced spindle activity that correlates with impaired sleep-dep

29 are transformed into the diversity of muscle spindle afferent firing patterns observed experimentally

30 We have shown that functional muscle spindle afferents are absent in the upper and lower limb

31 dysautonomia, do not have functional muscle spindle afferents but do have essentially normal cutaneo

32 tial exploration were engaged in SWR-coupled spindles after behavior.

33 nimal cells are able to orient their mitotic spindles along their interphase cell axis, setting up th

34 lations and their coordination with thalamic spindles, an interregional dialog that is necessary for

35 The dynamic spindle anchors kinetochore-fibers in space and time to

36 veal a dynamic two-way crosstalk between the spindle and cortical motor complexes that depends on a R

37 Using in silico approaches and various spindle and DNA perturbations, we show that chromosomes

38 pport these processes as well, including the spindle and kinetochore-associated (Ska) complex.

39 using a phenomenological model of the muscle spindle and muscle lengths derived from a musculoskeleta

40 zed orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old

41 homeostatic gene regulatory networks within spindle and root cells, establishing a basis for underst

42 ctra derived from multivariate analysis, and spindle and slow oscillation morphology and coupling.

43 Spindle and SO "nesting," or the temporal overlap betwee

44 GEMs was similar inside the dense metaphase spindle and the surrounding cytoplasm.

45 organize the microtubule network and mitotic spindle and, as basal bodies, nucleate cilia and flagell

46 ector of branching microtubule nucleation in spindles and functions with the substrate tubulin by an

47 rely on interactions between thalamocortical spindles and hippocampal ripples.

48 ampal-cortical dialogue is mediated by sleep spindles and is enhanced during long-duration hippocampa

49 sions and reduced hierarchical coupling with spindles and ripples.

50 Spindles and slow oscillations (SOs) both appear to play

51 Cdc23 localized on the meiotic spindle, and microinjection of Cdc23 siRNA caused decrea

52 ) collapsed, 2) monopolar, and 3) multipolar spindles, and the computational screen reveals mechanica

53 ter, determines the eventual location of the spindle apparatus and ultimately the cytokinetic furrow.

54 yotes requires the regulated assembly of the spindle apparatus.

55 mechanics that determines three-dimensional spindle architecture.

56 We conclude that spindles are shaped by the interplay between surface ten

57 Sleep processes, particularly fast sleep spindles, are thought to support consolidation, but evid

58 roteins that play important roles in mitotic spindle assembly [1].

59 The essential functions required for mitotic spindle assembly and chromosome biorientation and segreg

60 that we previously found to control mitotic spindle assembly and chromosome dynamics.

61 ing microtubule density and organization and spindle assembly and function and in activating some of

62 y highlight that kindlin-2 regulates mitotic spindle assembly and that this process is perturbed in c

63 T-severing activity is essential for meiotic spindle assembly but is toxic for the mitotic spindle.

64 Defects in the spindle assembly checkpoint (SAC) can lead to aneuploidy

65 known as the corona, where it scaffolds the spindle assembly checkpoint (SAC) machinery by binding d

66 The spindle assembly checkpoint (SAC) prevents premature chr

67 atory chromosome passenger complex (CPC) and spindle assembly checkpoint (SAC) proteins in Drosophila

68 The spindle assembly checkpoint (SAC) relies on the recruitm

69 w chromatid cohesion defects and an impaired spindle assembly checkpoint (SAC), thus undergoing mitot

70 es to silence the mitotic checkpoint (a.k.a. spindle assembly checkpoint [SAC]).

71 hromosome segregation through regulating the spindle assembly checkpoint activity, and cyclin B1 and

72 Further study showed that inactivation of spindle assembly checkpoint and degradation of Cyclin B1

73 Furthermore, we found that inhibiting spindle assembly checkpoint protein Msp1 partly rescued

74 ora B-dependent spindle assembly, but not in spindle assembly checkpoint signaling at unattached kine

75 taining complexes from cells under different spindle assembly checkpoint signaling conditions.

76 s Bub1 and BubR1 kinetochore recruitment and spindle assembly checkpoint signaling.

77 umulation is increased by suppression of the spindle assembly checkpoint, suggesting this effect resu

78 around chromatin is important for governing spindle assembly during meiosis and mitosis by releasing

79 ha and beta gradually tune the activities of spindle assembly factors.

80 Here we show that acentrosomal spindle assembly following PLK4 inhibition depends on le

81 contribution of tubulin and microtubules to spindle assembly has been limited by the fact that physi

82 asts and human cancer cells to study mitotic spindle assembly in polyploid cells, we found that most

83 atiotemporally regulated to ensure efficient spindle assembly remains unclear.

84 ated TRIM37 expression inhibits acentrosomal spindle assembly through a distinct mechanism that invol

85 from retrograde axonal transport to mitotic spindle assembly(1,2).

86 ess cells that exhibit delayed, acentrosomal spindle assembly(4).

87 romosomal recruitment and Aurora B-dependent spindle assembly, but not in spindle assembly checkpoint

88 ecular motors cross-talk to regulate initial spindle assembly, we use a combination of micropatternin

89 lp focus spindle poles for efficient bipolar spindle assembly.

90 (an ortholog of kindlin-2) prevents abnormal spindle assembly; however, the mechanism remains unknown

91 nd MAD1L1 suggest a possible role of mitotic spindle-assembly genes in IPF susceptibility.

92 arly distinct responses to loss of different spindle-associated genes and underscore the importance o

93 ns in the phragmoplast and uncovered a novel spindle-associated microtubule motor protein.

94 on during cell division is driven by mitotic spindle attachment to the centromere region on each chro

95 issegregation caused by incorrect chromosome-spindle attachments.

96 es allows the formation of stable amphitelic spindle attachments.

97 e spindle, it is important to understand how spindles become oriented.

98 , soft matter approaches have shown that the spindle behaves as an active liquid crystal.

99 Importantly, these differences in spindle behavior outside and inside the midline can be r

100 s is known about the structures that mediate spindle bipolarization in mammalian oocytes.

101 mouse oocytes, kinetochores are required for spindle bipolarization in meiosis I.

102 fic function of kinetochores in acentrosomal spindle bipolarization in mice, and provides insights in

103 Human oocytes, where spindle bipolarization is reportedly error prone, exhibi

104 The kinetochore-dependent mode of spindle bipolarization is required for meiosis I to prev

105 epresents a gametogenic challenge, requiring spindle bipolarization without predefined bipolar cues.

106 GTSE1 recruited to the spindle by clathrin stabilizes microtubules by inhibitin

107 somal, yet they are able to assemble bipolar spindles by clustering centrosomes into two spindle pole

108 Kinesin-5 motors organize mitotic spindles by sliding apart microtubules.

109 sively reorganized so that a bipolar mitotic spindle can be correctly assembled.

110 much the primary afferent activity of muscle spindles can contribute to shaping muscle coactivation p

111 peripheral nerve sheath tumor (C-MPNST) and spindle cell melanoma (SCM) have not been well elucidate

112 s is a cell of endothelial origin termed the spindle cell.

113 Remarkably, bacteriocytes turn into spindle cells and migrate along the midgut epithelium, t

114 Spindle cells are predominantly latently infected with o

115 s that often yield non-diagnostic results or spindle cells on fine needle aspiration biopsy.

116 hort-lived, specialized reinforcement in the spindle center.

117 he preservation of local architecture in the spindle-center over seconds.

118 SC1 belongs, comprises genes involved in the spindle checkpoint (BUB1, MAD1, BIM1, and KAR3), and the

119 tablished that this function is required for spindle checkpoint activation, we demonstrate that in ce

120 e master mitotic regulator cyclin B1 and the spindle checkpoint component Mad1 was independently desc

121 We demonstrate that spindle checkpoint genes act upstream of Isc1, and their

122 to act downstream of Isc1, thus coupling the spindle checkpoint genes and Isc1 to CDC55-mediated nucl

123 volume, PCH-2 is no longer required for the spindle checkpoint or recruitment of Mad2 at unattached

124 These CDC20(6A) cells show a normal spindle checkpoint response and rapidly destroy cyclin B

125 hese forces with extensive forces to prevent spindle collapse.

126 Clearly, the lack of muscle spindles compromised proprioception at the knee but not

127 We also find that three other spindle configurations emerge if these conditions are no

128 airwise correlations increased during nested spindles, consistent with targeted strengthening of func

129 Embryonic zebrafish spindles contain asymmetrically sized mitotic centrosome

130 f the system that recapitulates the observed spindle-cortex interactions.

131 d elevated levels of hippocampal-neocortical spindle coupling around ripples, with directionality ana

132 oupling approach, which demonstrates that SO-spindle coupling strength increases during maturation.

133 pressed slow oscillations (SOs), and SO-fast spindle coupling was observed.

134 rates endogenous Spc110 from the SPB causing spindle defects.

135 dria, thus generating a counter force on the spindle, demonstrated an inherent ability of this system

136 d whether eszopiclone could both increase N2 spindle density and improve memory.

137 Patients showed a widespread reduction of spindle density and, in both groups, eszopiclone increas

138 y and, in both groups, eszopiclone increased spindle density but failed to enhance sleep-dependent pr

139 an fMRI data (both sexes), we show that fast spindle density during overnight sleep is related to enh

140 ate pattern analyses, we also show that fast spindle density during postlearning sleep is associated

141 Spindle density modulated connectivity in distinct hippo

142 ss a hypothesis that rationalizes changes in spindle design with spindle size based on the negative e

143 tical dynein, the precise mechanism by which spindles detect and align with the long cell axis remain

144 ecruitment of downstream ESCRTs, compromised spindle disassembly, and led to defects in nuclear integ

145 rns the activation of ESCRTs and coordinated spindle disassembly.

146 e to recruit MTOC components and liquid-like spindle domain (LISD) factors.

147 The results provide critical evidence that spindles during overnight sleep may act as a physiologic

148 eads to disorientation of Plasmodium mitotic spindles during the asexual reproduction and results in

149 pling may cause impaired learning in AD, and spindle-DW coupling during short rest-task-rest sessions

150 However, in AD mice, SWR-DW and spindle-DW coupling were impaired.

151 trikingly, when this model was used to study spindle dynamics in cells entering mitosis, the chromati

152 Ran in mouse oocytes while examining the MI spindle dynamics.

153 activity to control the rate and duration of spindle elongation during anaphase is poorly understood.

154 o the midzone before anaphase onset and slow spindle elongation during early anaphase.

155 lar to BUB1 deletion, ISC1 deletion prevents spindle elongation in hydroxyurea-treated cells.

156 t microtubule-binding domains to control the spindle elongation rate.

157 ric chromatin to microtubules of the mitotic spindle, enabling sister chromatid segregation in mitosi

158 orm a framework that interdigitates near the spindle equator.

159 Metaphase spindles exert pole-directed forces on still-connected s

160 Lack of muscle spindle feedback from the legs may account for the poor

161 s of spindle positioning and elongation, and spindle final length and scaling with cell size.

162 e assembly and organization of a microtubule spindle for the proper separation of chromosomes in mito

163 The primary spindle force generators are kinesin-5 motors and crossl

164 1, a kinesin motor protein, promotes bipolar spindle formation and chromosome movement, and during in

165 n; cells then underwent additional rounds of spindle formation and disassembly without DNA re-replica

166 The dynamics of spindle formation are determined primarily by correctly

167 ntributions of an EB1-Kinesin-14 complex for spindle formation as a prerequisite for efficient kineto

168 ion, which may facilitate rapid and accurate spindle formation.

169 Migration of meiosis-I (MI) spindle from the cell center to a sub-cortical location

170 among different classes of MTs in metaphase spindles from Chlamydomonas rheinhardti and two strains

171 rgo binding to importin beta and disrupts MI spindle function in chromosome segregation.

172 nd whether its perceived role stems from its spindle function, are unclear.

173 ds to the muscle (alpha drive) versus muscle spindle (gamma drive) can cause highly variable and comp

174 Although sleep spindles have been associated with benefits in memory re

175 n firing characteristics of mammalian muscle spindle Ia afferents - including movement history depend

176 e to be met to robustly assemble the bipolar spindle in a multicentrosomal cell: 1) the strengths of

177 eres in mitosis and subsequently the central spindle in anaphase.

178 re of the NE opening surrounding the meiotic spindle in C. elegans oocytes.

179 phic reconstructions of spermatocyte meiotic spindles in Caenorhabditis elegans, we find the lagging

180 The lack of conventional muscle spindles in face muscles raises the question of how sens

181 n in even bigger spindles, such as metaphase spindles in Haemanthus endosperm and frog egg extracts.

182 halamic units were phase-locked to delta and spindles in mPFC, and fired at consistent lags with othe

183 Overall, this work establishes the spindle-independent function of NuMA in choreographing p

184 ive imaging to demonstrate that NuMA plays a spindle-independent role in forming a single, round nucl

185 e that improved coordination between SOs and spindles indexes the development of sleep-dependent memo

186 ncluding translation and ribosomal events in spindle, inflammation- and apical junction-related prote

187 Here, we show that kindlin-2 maintains spindle integrity in mitotic human cells.

188 The shift of patients' spindles into the later phase of the up-state within the

189 The metaphase spindle is a dynamic structure orchestrating chromosome

190 Bipolarity of the spindle is crucial for the proper cell division, and two

191 rk illustrates that RanGTP regulation in the spindle is not simply a switch, but rather generates eff

192 onsolidation in schizophrenia, but enhancing spindles is not enough.

193 largely set by the position of the anaphase spindle, it is important to understand how spindles beco

194 In small spindles, kinetochore microtubules (KMTs) connect direct

195 In all these spindles, KMTs approach close to and cross-bridge with t

196 broad classes of models of the regulation of spindle length and dynamics, and to establish the import

197 We show that spindle length and microtubule mass can be controlled by

198 Large spindle length fluctuations can occur when the kinetocho

199 Mitotic spindle length, for example, varies several-fold among c

200 g species influence microtubule dynamics and spindle length.

201 rotubule mass and therefore set steady-state spindle length.

202 changes in cell size compared to changes in spindle length.

203 generation contributes to its characteristic spindle-like shape.

204 The tail is essential for mitotic spindle localization, which becomes severely reduced in

205 ve that in the latter cells, an imbalance of spindle-mediated force and the simultaneous persistence

206 omain and are well described to organize the spindle microtubule during mitosis using an additional m

207 on depends on a regulated connection between spindle microtubules and centromeric DNA.

208 which promote the proper interaction between spindle microtubules and chromosomes.

209 Dynamic interaction between spindle microtubules and the kinetochore complex that as

210 on the proper attachment of kinetochores to spindle microtubules before anaphase onset.

211 ulating attachments between kinetochores and spindle microtubules during mitosis.

212 and provides the major attachment point for spindle microtubules during mitosis.

213 inetochores connect centromeric chromatin to spindle microtubules during mitosis.

214 vision, the mammalian kinetochore binds many spindle microtubules that make up the kinetochore-fiber.

215 otubules and targets condensation of LEM2 to spindle microtubules that traverse the nascent nuclear e

216 cluding 1) connecting mitotic chromosomes to spindle microtubules to establish force-transducing kine

217 erved machinery that connects chromosomes to spindle microtubules.

218 ctly adjacent to NE holes containing meiotic spindle microtubules.

219 dle-periphery localized FMN2 is required for spindle migration.

220 n the neuromechanical conditions, the muscle spindle model output appears to 'encode' aspects of musc

221 The parameters of the spindle model were altered systematically to evaluate th

222 ory retention, it is not well understood how spindles modify neural memory traces.

223 otein localization to centrosomes and impair spindle morphogenesis and genome stability.

224 SO and spindle morphology changes considerably throughout devel

225 is sufficient to drive continuous monopolar spindle motion independently of adhesive cues in flatten

226 tem to break symmetry and evolve directional spindle motion.

227 Problems with establishing predetermined spindle MTOC inheritance patterns during stem cell divis

228 After duplication, spindle MTOCs can be differentially inherited during asy

229 Augmin, recruits gamma-TuRC to pre-existing spindle MTs, amplifying their number, in an essential ce

230 The spindle must counter these forces with extensive forces

231 (OR 7.78; 95% CI, 2.69-22.35, P < .001), rod/spindle objects (OR 7.05; 95% CI, 2.11-23.59, P = .002),

232 Absence of rod/spindle objects was associated with a BCVA of >=6/6 (OR

233 -coil protein that plays a prominent role in spindle organization during mitosis.

234 microtubule bundler Ase1/PRC1 for metaphase spindle organization, and simultaneous loss of plus-end

235 ent regulates Kinesin-14 function to control spindle organization.

236 have been identified that play key roles in spindle orientation across systems, most notably Mud/NuM

237 Here, in exploring the dynamics of spindle orientation in mechanically distinct regions of

238 or function/localization that alters mitotic spindle orientation, chromosomal segregation, and nuclea

239 The microtubules that form the mitotic spindle originate from microtubule-organizing centers (M

240 In bigger spindles, particularly those without structured poles, t

241 f nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependen

242 nt of phosphorylated CYK4 around the central spindle patterns RhoA activation by interacting with ECT

243 ural synchrony, or correlated firing, during spindle peaks.

244 responsible for targeting FMN2, we show that spindle-periphery localized FMN2 is required for spindle

245 large zebrafish embryonic centrosomes direct spindle placement within disproportionately large cells.

246 ed to maintain correct centrosome number and spindle polarity in cells.

247 Spindles polarized, pairs of daughter cells oriented bet

248 ere we describe how scaffolding the MEN onto spindle pole bodies (SPB-centrosome equivalent) allows t

249 dc13 to the yeast centrosome equivalent, the spindle pole body (SPB), and disruption of this motif pr

250 primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud

251 romosomes act as a physical barrier blocking spindle pole coalescence and bipolarity.

252 s did not separate and did not move toward a spindle pole.

253 llel microtubule cross-linking to help focus spindle poles for efficient bipolar spindle assembly.

254 asses of chromatin thus ended up at opposite spindle poles, giving the appearance of successful anaph

255 spindles by clustering centrosomes into two spindle poles.

256 Gravin binding partner polo-like kinase 1 at spindle poles.

257 rosomes in animal cells naturally become two spindle poles.

258 MEN to couple the final stages of mitosis to spindle position.

259 ic rounding, which is important for accurate spindle positioning and chromosome separation.

260 ubule), is key to explaining the dynamics of spindle positioning and elongation, and spindle final le

261 Factors that regulate mitotic spindle positioning remain unclear within the confines o

262 We first observed a concurrent spindle power increase in hippocampus (HIPP) and neocort

263 During spindles, primary motor cortex neurons fired at a prefer

264 ripheral targets and reconstitute the muscle spindle proprioceptive receptors.

265 NuMA is a spindle protein whose disruption results in nuclear frag

266 rm-associated Antigen 5 (SPAG5) is a mitotic spindle protein.

267 We report that the cycles of neocortical spindles provide a key temporal window that coordinates

268 Whether fast spindles provide a mechanism for neural changes hypothes

269 In contrast to other known spindle-regulating genes, Shot knockdown induces apoptos

270 chments from transient forces while allowing spindle remodeling, and chromosome movements, over longe

271 Thus, spindles reorient to align with the long cell axis in re

272 indicate a mismatch in timing across the SO-spindle-ripple events that are associated with memory co

273 bule dynamics [5-8], the contribution of the spindle's main building block, the alphabeta-tubulin het

274 vors the establishment of an initial bipolar spindle scaffold, facilitating chromosome capture and ac

275 ot comprised poorly differentiated, round to spindle-shaped cells with prominent neutrophilic infiltr

276 ighly vascular and red/purple tumor lesions, spindle-shaped cells, an insignificant role for classic

277 Sphere-like aggregates and spindle-shaped structures were, respectively, formed fro

278 tance of Sulfolobus islandicus to Sulfolobus spindle-shaped virus (SSV9) conferred by chromosomal del

279 The spindle shows remarkable diversity, and changes in an in

280 rationalizes changes in spindle design with spindle size based on the negative exponential distribut

281 Although most studies on spindle size control focus on changes in proteins that r

282 Spindle size depends primarily on microtubule number, wh

283 gements of MTs have been seen in even bigger spindles, such as metaphase spindles in Haemanthus endos

284 Together, these results suggest that fast spindles support the network distribution of memory trac

285 on by creating a docking site on the central spindle that concentrates the RhoA guanine nucleotide ex

286 re known to underlie the generation of sleep spindles, the mechanisms regulating slow (<1 Hz) forms o

287 The KSP inhibitor targets the mitotic spindle through mechanisms independent of microtubule st

288 etochore-microtubule interface and along the spindle to control chromosome segregation.

289 ctions including organization of the mitotic spindle to ensure faithful chromosome segregation during

290 his model accounts for variations in all the spindle traits we studied here, both within species and

291 Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive appro

292 osomes and simultaneously assemble a bipolar spindle, we developed a computational model of fission-y

293 Although we observed no spindles, we found a clear state of intermediate sleep (

294 In contrast, slow and fast spindles were indistinguishable from those of control pa

295 In control mice, spindles were phase-coupled with DWs, and hippocampal SW

296 o a cortical pattern that can be read by the spindle, which then guides the axis of cell division.

297 istent lags with other thalamic units within spindles, while CA1 units that were active during spatia

298 rther, mechanical interactions of the muscle spindle with muscle-tendon dynamics reveal how motor com

299 pulling on astral microtubules align bipolar spindles with the interphase long cell axis, without req

300 closer scrutiny, we noted that the timing of spindles within the SO cycle was delayed in the patients