ライフサイエンスコーパス: spindle checkpoint

1 re in both the chromosome attachment and the spindle checkpoint.

2 ome segregation, and optimal function of the spindle checkpoint.

3 e attachments and subsequently silencing the spindle checkpoint.

4 itive tumors and associated with the mitotic spindle checkpoint.

5 APC/C(Cdc20) is the target of the spindle checkpoint.

6 motor associated, are needed to silence the spindle checkpoint.

7 , it inhibits mitotic exit by activating the spindle checkpoint.

8 y and is essential for the activation of the spindle checkpoint.

9 t Aurora B kinase to efficiently silence the spindle checkpoint.

10 te of mitotic slippage and adaptation to the spindle checkpoint.

11 neral role of the APC protein in the mitotic spindle checkpoint.

12 to identify lead compounds that inhibit the spindle checkpoint.

13 l1 targets contribute to segregation and the spindle checkpoint.

14 of the final steps of mitosis--silencing the spindle checkpoint.

15 tive spindle formation and activation of the spindle checkpoint.

16 of bi-oriented attachments and activated the spindle checkpoint.

17 e formation and subsequent activation of the spindle checkpoint.

18 across sister kinetochores and activate the spindle checkpoint.

19 biquitin ligase, Smurf2, is required for the spindle checkpoint.

20 uces mitotic arrest due to activation of the spindle checkpoint.

21 found that E6 expression does not affect the spindle checkpoint.

22 protease 44) as a critical regulator of the spindle checkpoint.

23 o promoting robust activation of the mitotic spindle checkpoint.

24 cells to arrest in mitosis with a functional spindle checkpoint.

25 ing that LCMT-1 and Balpha are important for spindle checkpoint.

26 phase chromosome alignment and activates the spindle checkpoint.

27 elieved to function primarily in the mitotic spindle checkpoint.

28 of APC in U2OS cells compromises the mitotic spindle checkpoint.

29 iple roles in chromosome segregation and the spindle checkpoint.

30 sion of the APC in anaphase, reactivates the spindle checkpoint.

31 ase activity of Aurora B, a regulator of the spindle checkpoint.

32 sted to stabilise attachment and silence the spindle checkpoint.

33 in microtubule binding and regulation of the spindle checkpoint.

34 is known about its functions apart from the spindle checkpoint.

35 hat determines kinetochore activation of the spindle checkpoint.

36 izes to mitotic centrosomes and controls the spindle checkpoint.

37 dination of chromosome biorientation and the spindle checkpoint.

38 ting an interplay between the DNA damage and spindle checkpoints.

39 chores and microtubules are monitored by the spindle checkpoint, a surveillance system that prevents

40 checkpoint proteins to the kinetochore upon spindle checkpoint activation are incompletely understoo

41 Although the molecular details of spindle checkpoint activation are still being elucidated

42 st, they are uniquely sensitive to transient spindle checkpoint activation as a result of a failure i

43 unattached kinetochores and is required for spindle checkpoint activation in Caenorhabditis elegans.

44 Glc7 overexpression prevents spindle checkpoint activation in response to both tensio

45 The phenotype is neither caused by spindle checkpoint activation nor a defect in the assemb

46 explanation of how cells die after prolonged spindle checkpoint activation, and thus how microtubule

47 tablished that this function is required for spindle checkpoint activation, we demonstrate that in ce

48 activity and is the most downstream event in spindle checkpoint activation.

49 ntial for Mad2 targeting to kinetochores and spindle checkpoint activation.

50 events and prevents mitotic slippage during spindle checkpoint activation.

51 f checkpoint silencing, is also critical for spindle checkpoint activation.

52 The spindle checkpoint acts as a mitotic surveillance system

53 The spindle checkpoint acts during cell division to prevent

54 cle checkpoint after DNA damage stress and a spindle checkpoint after microtubule disruption.

55 that E6 induces polyploidy by abrogating the spindle checkpoint after mitotic stress.

56 levated in mitosis or upon activation of the spindle checkpoint, although the dynamic range of Mps1 e

57 B1 and securin, is a bona fide target of the spindle checkpoint and APC/C(Cdc20).

58 spond to microtubule drugs by activating the spindle checkpoint and arresting in mitosis with a round

59 rigenesis; however, the relation between the spindle checkpoint and cell death remains obscure.

60 s to the Bub1 kinase, a key regulator of the spindle checkpoint and chromosome segregation.

61 gression, as knockdown of Bora activates the spindle checkpoint and delays sister chromatid segregati

62 1 phosphorylation sites are defective in the spindle checkpoint and exhibit growth defects.

63 that is able to alter the properties of the spindle checkpoint and initiate a signal transduction ca

64 ndle attachments that evade detection by the spindle checkpoint and persist into anaphase.

65 that binds weakly to PP1 also activates the spindle checkpoint and suppresses the temperature sensit

66 cycle by numerous mechanisms, including the spindle checkpoint and the cytostatic factor (CSF).

67 In cells lacking KIF4, mitotic spindle checkpoints and DNA-damage response pathways are

68 ing G2 permits the optimal activation of the spindle checkpoint, and consequently it is required for

69 of MTs in a kinetochore fiber, activates the spindle checkpoint, and delays the mitotic progression.

70 in shortened mitotic timing and a defective spindle checkpoint, and that abrogation of ATM Ser1403 p

71 We conclude that the spindle checkpoint, APC/C(Cdc20), and APC/C(Cdh1) act su

72 Biorientation and the spindle checkpoint are regulated by a balance between th

73 on of checkpoint complexes, we conclude that spindle checkpoint arrest can be independent of their ki

74 se activity is directly required to maintain spindle checkpoint arrest, even in the presence of many

75 hores during mitotic entry and for sustained spindle checkpoint arrest.

76 pl1 kinase to detach kinetochores and induce spindle checkpoint arrest.

77 for spindle assembly and maintenance of the spindle checkpoint arrest.

78 biorientation upon recovery from a prolonged spindle checkpoint arrest.

79 ortant for spindle formation and the mitotic spindle checkpoint arrest.

80 The spindle checkpoint arrests cells in metaphase until all

81 that B-Raf(V600E) signaling deregulates the spindle checkpoint as a consequence of stabilizing monop

82 cts strictly depends on a functional mitotic spindle checkpoint as well as on intact microtubule pull

83 Etr in the development of aneuploidy through spindle checkpoint attenuation.

84 metaphase arrest is mediated by the mitotic spindle checkpoint being dependent on Mad1 and the Auror

85 The spindle checkpoint blocks cell-cycle progression until c

86 SC1 belongs, comprises genes involved in the spindle checkpoint (BUB1, MAD1, BIM1, and KAR3), and the

87 val of defective nuclei does not require the spindle checkpoint but instead depends on the DNA damage

88 knockdown cells can enter mitosis and retain spindle checkpoint, but fail to complete cytokinesis.

89 in kinase that regulates segregation and the spindle checkpoint, but few of the targets that mediate

90 kinase activity of Mps1 is required for the spindle checkpoint, but how Mps1 is activated during mit

91 dynein binds directly to a component of the spindle checkpoint complex through the DYNLT3 light chai

92 The first pathway involves the Mad1-Mad2 spindle checkpoint complex, acting in a distinct manner

93 e master mitotic regulator cyclin B1 and the spindle checkpoint component Mad1 was independently desc

94 The history of Bub1, a spindle checkpoint component, reveals a spectacular case

95 nucleoplasm, yet kinetochore localization of spindle checkpoint components is required for proper rec

96 s for other B-type cyclins in the absence of spindle checkpoint components.

97 ed by differential genetic interactions with spindle checkpoint components.

98 s a conserved mitotic regulator critical for spindle checkpoint control, kinetochore functionality, a

99 The spindle checkpoint controls the fidelity of chromosome s

100 If this is the case, it is unclear how the spindle checkpoint could be satisfied.

101 of ATM Ser1403 phosphorylation leads to this spindle checkpoint defect.

102 The spindle checkpoint delays anaphase onset in cells with m

103 The spindle checkpoint delays anaphase onset until all chrom

104 The spindle checkpoint delays exit from mitosis in cells wit

105 In metazoans, plants, and fungi, the spindle checkpoint delays mitosis until each chromosome

106 nents is required for proper recovery from a spindle checkpoint-dependent arrest.

107 We show that mitotic errors result in a spindle checkpoint-dependent cell-cycle delay, but defec

108 turb spindle dynamics, and induce prolonged, spindle checkpoint-dependent mitotic arrest in cancer ce

109 l Ca(2+) transients via MCU depletion causes spindle checkpoint-dependent mitotic delay.

110 In mitosis, the spindle checkpoint detects a single unattached kinetocho

111 The spindle checkpoint detects unattached chromosomes and mo

112 The spindle checkpoint ensures accurate chromosome segregati

113 The spindle checkpoint ensures accurate chromosome segregati

114 The spindle checkpoint ensures accurate chromosome transmiss

115 The spindle checkpoint ensures the accuracy of chromosome se

116 The spindle checkpoint ensures the proper partition of the c

117 hore also serves as a regulatory hub for the spindle checkpoint, ensuring that cell cycle progression

118 B1 is therefore an integral component of the spindle checkpoint, ensuring the fidelity of mitosis.

119 partial Mps1 inhibition and was required for spindle checkpoint establishment at the beginning of mit

120 point activation as a result of a failure in spindle checkpoint exit.

121 Spindle checkpoint failure in Chk1-deficient cells corre

122 The spindle checkpoint forms an intricate signaling circuit

123 for kinetochore-microtubule interactions and spindle checkpoint function [3-7]; however, the underlyi

124 lls, Mps1 catalytic activity is required for spindle checkpoint function and recruitment of Mad2.

125 culture or transgenic mice inhibited mitotic spindle checkpoint function because of reduced Rb transc

126 aling proteins, but Plk1 is not required for spindle checkpoint function.

127 ed to functions of Bub1/3 unrelated to their spindle checkpoint function.

128 Mad2) have been proposed to be essential for spindle checkpoint function.

129 es not seem to be due to compromised mitotic spindle checkpoint function.

130 tic human cells but is capable of supporting spindle-checkpoint function.

131 Deletion of the spindle checkpoint gene mdf-1 blocks lis-1(lf)-induced c

132 However, another spindle checkpoint gene, MAD2, could be deleted without

133 The spindle checkpoint generates a "wait anaphase" signal at

134 We demonstrate that spindle checkpoint genes act upstream of Isc1, and their

135 to act downstream of Isc1, thus coupling the spindle checkpoint genes and Isc1 to CDC55-mediated nucl

136 Deletion of the spindle checkpoint genes BUB1 and BUB3 caused synthetic

137 e systems--the DNA damage checkpoint and the spindle checkpoint--guard against genomic instability.

138 The spindle checkpoint halts the cell cycle in response to d

139 ficient) cells, conditions that activate the spindle checkpoint (i.e., cold shock or treatment with n

140 kinase, Mps1, is a critical component of the spindle checkpoint in human cells and regulates the kine

141 The KEN boxes of Bub1 are required for the spindle checkpoint in human cells.

142 orylation sites in Bub1 or Mad1 abrogate the spindle checkpoint in human cells.

143 regulates normal mitotic progression and the spindle checkpoint in response to spindle damage.

144 The APC/C is inhibited by the spindle checkpoint in the presence of kinetochore attach

145 rs Emi1 and BubR1 in vitro, and bypasses the spindle checkpoint in vivo.

146 epletion of individual APC/C subunits or the spindle checkpoint inhibitor p31(comet).

147 Thus, the spindle checkpoint is "uncoupled" from apoptosis in ESCs

148 The spindle checkpoint is a cell cycle surveillance system t

149 The spindle checkpoint is a conserved signaling pathway that

150 The spindle checkpoint is a surveillance system acting in mi

151 an APCCdc20 substrate, is degraded when the spindle checkpoint is active, while other APCCdc20 subst

152 e degraded during spindle formation when the spindle checkpoint is active.

153 The spindle checkpoint is essential to ensure proper chromos

154 d, bipolar spindles fail to assemble but the spindle checkpoint is inappropriately silenced due to PP

155 The spindle checkpoint is responsible for arresting cells in

156 d cells exhibit slower mitotic exit when the spindle checkpoint is silenced by inhibition of the chec

157 nts to microtubules from opposite poles, the spindle checkpoint is silenced.

158 , suggesting that APCCdc20 inhibition by the spindle checkpoint is substrate specific.

159 The target of the spindle checkpoint is the anaphase-promoting complex (AP

160 The spindle checkpoint is the prime cell-cycle control mecha

161 d regulating microtubule attachments and the spindle checkpoint, is discussed.

162 ciency 2 (Mad2), a critical component of the spindle checkpoint, is overexpressed in many cancer cell

163 Finally, we show that the spindle checkpoint kinase Bub1 contributes to the mainte

164 The master spindle checkpoint kinase Mps1 senses kinetochore-microt

165 Several spindle checkpoint kinases participate in the generation

166 Because complete absence of the spindle checkpoint leads to catastrophic cell division,

167 t responses to two insults that activate the spindle checkpoint: MAD2/mad2Delta cells respond normall

168 The spindle checkpoint maintains genomic stability and preve

169 ese results suggest that inactivation of the spindle checkpoint may contribute to the development of

170 Its apparent dispensability in the spindle checkpoint might be due to functional duality or

171 study, we develop evidence that the mitotic spindle checkpoint molecule BUB1B may offer a predictive

172 The spindle checkpoint monitors kinetochore-microtubule inte

173 The mitotic spindle checkpoint monitors proper bipolar attachment of

174 The spindle checkpoint monitors the attachment of spindles t

175 malorientations that are not detected by the spindle checkpoint; Ndel1-deficient cells consequently e

176 ality reflected some function other than the spindle checkpoint of Bub1 and Bub3.

177 The spindle checkpoint of the cell division cycle senses kin

178 metaphase alignment but fail to silence the spindle checkpoint or enter anaphase.

179 volume, PCH-2 is no longer required for the spindle checkpoint or recruitment of Mad2 at unattached

180 les in egg formation and apoptosis (p63) and spindle checkpoint (p73) in female mice.

181 5 (WHI5), and the FEL lost components of the spindle checkpoint pathway (e.g., Mitotic Arrest-Deficie

182 i revealed significant overrepresentation of spindle checkpoint pathway genes.

183 Because the spindle checkpoint pathway is highly conserved between y

184 e arrest and germline apoptosis, placing the spindle checkpoint pathway upstream of the programmed ce

185 nt and not only an upstream regulator of the spindle checkpoint pathway.

186 ether, CDK1-CCNB1 and PP2A-B55 thus create a spindle checkpoint-permissive state and ensure the fidel

187 We found four candidate spindle checkpoint phosphatases, including the tumor sup

188 ecombination, sister chromatid cohesion, the spindle checkpoint, postreplication repair, and telomere

189 The spindle checkpoint prevents aneuploidy by delaying anaph

190 The spindle checkpoint prevents chromosome mis-segregation b

191 The spindle checkpoint prevents sister chromatids from separ

192 used budding yeast to determine whether the spindle checkpoint promotes segregation of such chromoso

193 w here that PKM2, but not PKM1, binds to the spindle checkpoint protein Bub3 during mitosis and phosp

194 study clarifies the role of APC as a mitotic spindle checkpoint protein in vivo and shows that APC-de

195 of anaphase requires an understanding of how spindle checkpoint protein interaction with the kinetoch

196 d Pdr3; the mRNA processing factor Fir1; the spindle checkpoint protein kinase Mps1; and a protein of

197 ell biological analysis demonstrate that the spindle checkpoint protein MAD1 is one of the major comp

198 Molecular Cell, Cairo et al. report that the spindle checkpoint protein Mad1 shuttles between unattac

199 rotein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active

200 The spindle checkpoint protein Mad2 is an unusual protein wi

201 In many eukaryotes, disruption of the spindle checkpoint protein Mad2 results in an increase i

202 the microtubule-sensitive recruitment of the spindle checkpoint protein Mad2, and the segregation beh

203 ev1p and Mad2) domain similar to that of the spindle checkpoint protein Mad2.

204 f a downstream target for cyclin E/Cdk2, the spindle checkpoint protein Mps1, provides insight into h

205 Kinetochore architecture and spindle checkpoint protein recruitment are unaffected in

206 KSHV, can induce the degradation of Bub1, a spindle checkpoint protein that is important for spindle

207 rthermore, our findings suggest that Bub1, a spindle checkpoint protein, serves as a scaffold or mole

208 We find that DYNLT3 binds to Bub3, a spindle checkpoint protein.

209 Here we show that spindle checkpoint proteins are severely depleted from u

210 er, AEtr cells had reduced levels of several spindle checkpoint proteins including BubR1 and securin

211 We observed the dynamic recruitment of spindle checkpoint proteins Mad1 and Bub1 to detached ki

212 The spindle checkpoint proteins MAD2 and BUBR1 prevent preco

213 This in turn accentuates degradation of spindle checkpoint proteins such as BubR1 and Bub1, cont

214 ractions between the kinetochore and various spindle checkpoint proteins that ensure that sister chro

215 or substrate 1 and 2 (IRS1/2) cooperate with spindle checkpoint proteins to promote insulin receptor

216 tension triggers recruitment of several key spindle checkpoint proteins to the kinetochore, which de

217 ugh its interaction with one of the critical spindle checkpoint proteins, Bub1, and the resulting deg

218 ts (Apc14 and Apc15) regulate association of spindle checkpoint proteins, in the form of the mitotic

219 Spindle checkpoint proteins, including Mad1, Mad2, Mad3

220 tion of the checkpoint kinase MPS1 and other spindle checkpoint proteins.

221 y on a kinetochore substrate, and loading of spindle checkpoint proteins.

222 gue in response to mitotic delay, leading to spindle checkpoint re-activation and lethal mitotic arre

223 These CDC20(6A) cells show a normal spindle checkpoint response and rapidly destroy cyclin B

224 t cannot bind aurora B, are sufficient for a spindle checkpoint response when microtubules are absent

225 Dysfunctional DNA damage and mitotic spindle checkpoints resulting from these interactions ma

226 esses microtubule dynamics and activates the spindle checkpoint, revealed a specific switch in kineto

227 Because other aspects of O. tauri's spindle checkpoint seem typical, these data suggest that

228 During mitosis, the spindle checkpoint senses kinetochores not properly atta

229 The spindle checkpoint senses unattached kinetochores and in

230 The spindle checkpoint senses unattached kinetochores during

231 The spindle checkpoint senses unattached or improperly attac

232 e a molecular switch that maintains a robust spindle checkpoint signal at prometaphase kinetochores u

233 associate with kinetochores to generate the spindle checkpoint signal, but they are released when a

234 ate tension and simultaneously maintains the spindle checkpoint signal.

235 dle checkpoint protein that is important for spindle checkpoint signaling and chromosome segregation.

236 We propose that Chk1 augments spindle checkpoint signaling and is required for optimal

237 n of mitotic chromosomes, where it initiates spindle checkpoint signaling and promotes chromosome ali

238 rdination between microtubule attachment and spindle checkpoint signaling at the kinetochore.

239 centromeres, outer kinetochore formation, or spindle checkpoint signaling but nevertheless elevates c

240 s activity of BUB-1/BUB-3 was independent of spindle checkpoint signaling but required kinetochore lo

241 Mps1 acts upstream in the spindle checkpoint signaling cascade, and kinetochore ta

242 The status of spindle checkpoint signaling depends on the balance of t

243 on, kinetochore-microtubule attachments, and spindle checkpoint signaling during mitosis.

244 teins responsible for lateral attachment and spindle checkpoint signaling expand to form micrometer-s

245 o systematically define the requirements for spindle checkpoint signaling in the Caenorhabditis elega

246 red for its kinase activity in vitro and for spindle checkpoint signaling in vivo.

247 Spindle checkpoint signaling is initiated by recruitment

248 CTD, recruits PP1 to kinetochores to oppose spindle checkpoint signaling kinases and promote anaphas

249 tes kinetochore-microtubule attachments with spindle checkpoint signaling on each mitotic chromosome.

250 equently, metaphase chromosome alignment and spindle checkpoint signaling were compromised.

251 east eight protein kinases are implicated in spindle checkpoint signaling, arguing that a traditional

252 veral protein kinases play critical roles in spindle checkpoint signaling, but the mechanism (or mech

253 Although critical for spindle checkpoint signaling, the role kinetochores play

254 roles of Aurora B in chromosome movement and spindle checkpoint signaling.

255 protein kinase that plays essential roles in spindle checkpoint signaling.

256 ts with the microtubule and is essential for spindle checkpoint signaling.

257 regulated during the cell cycle and mitotic spindle checkpoint signaling.

258 l signal transduction cascade is integral to spindle checkpoint signaling.

259 phosphorylation in kinetochore targeting and spindle checkpoint signaling.

260 ct against tumorigenesis through its role in spindle checkpoint signaling.

261 e spindle microtubule capture and to control spindle checkpoint signaling.

262 interaction with the kinetochore influences spindle checkpoint signaling.

263 t for TRIP13(PCH-2) in Mad2 localization and spindle checkpoint signaling.

264 mplexes to kinetochores and is essential for spindle checkpoint signaling.

265 nattached kinetochores is a central event in spindle checkpoint signaling.

266 required for proper assembly of the mitotic spindle, checkpoint signaling, and several other aspects

267 role of spatiotemporal regulation in mitotic spindle checkpoint signalling and fidelity of chromosome

268 Together, these results suggest that spindle checkpoint signals can be amplified in the nucle

269 The complex is also required to generate spindle checkpoint signals in both yeast and human cells

270 tochore-microtubule attachments and weakened spindle checkpoint signals.

271 Spindle checkpoint silencing is a critical step during m

272 Spindle checkpoint silencing is crucial for cell-cycle p

273 ation of checkpoint components forms a novel spindle checkpoint silencing mechanism.

274 tic insights and propose a working model for spindle checkpoint silencing.

275 Spindle checkpoint strength is dictated by the number of

276 ere consistent with faulty activation of the spindle checkpoint, such as shortened mitosis, premature

277 that Chk1 also plays a critical role in the spindle checkpoint, suggesting an interplay between the

278 hore-microtubule attachments to shut off the spindle checkpoint, suggesting that cells regulate the E

279 In mitosis, the spindle checkpoint supports Cdt1 accumulation, which pro

280 unction, which may be part of a more general spindle checkpoint surveillance mechanism.

281 g of Bub3 to the Spc7 MELT array toggles the spindle checkpoint switch by permitting Mph1 (Mps1)-depe

282 ivate a signaling pathway called the mitotic spindle checkpoint that blocks progression into anaphase

283 The spindle checkpoint that monitors kinetochore-microtubule

284 isalignment, mitotic catastrophe and loss of spindle checkpoint, that are accompanied by reduced expr

285 fficient execution of their functions in the spindle checkpoint, the self-monitoring system of the eu

286 ility to phosphorylate Bub1 and regulate the spindle checkpoint, thus maintaining genomic integrity.

287 Kinetochores use the spindle checkpoint to delay anaphase onset until all chr

288 an age-related decline in the ability of the spindle checkpoint to delay separase-mediated cleavage o

289 ely on a surveillance mechanism known as the spindle checkpoint to ensure accurate chromosome segrega

290 ated from these opposing forces silences the spindle checkpoint to ensure accurate chromosome segrega

291 ochore-microtubule attachments and alert the spindle checkpoint to the presence of misaligned chromos

292 At the metaphase-anaphase transition, the spindle checkpoint turns off, and MCC disassembles to al

293 ts the role of MK2 in G(2)/M and the mitotic spindle checkpoints, two mechanisms by which MK2 contrib

294 network plays a major role in silencing the spindle checkpoint when chromosomes are aligned at metap

295 Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chro

296 tometric analysis, we found that the mitotic-spindle checkpoint, which helps maintain chromosomal int

297 chromosome segregation is controlled by the spindle checkpoint, which senses kinetochore- microtubul

298 However, Tax did not affect the mitotic spindle checkpoint, which was also functional in HTLV-I-

299 deficient-like 1, MAD2L1, a component of the spindle checkpoint whose down-regulation is essential in

300 for how cancer cells can have a compromised spindle checkpoint without corresponding mutations in ch