ライフサイエンスコーパス: spindle microtubule

1 establishes stable bipolar attachment to the spindle microtubule.

2 centromere and the Ndc80p/Nuf2p end faces a spindle microtubule.

3 understanding the complete life history of a spindle microtubule.

4 mic interactions between the kinetochore and spindle microtubules.

5 otein, abnormal spindle (Asp), to cross-link spindle microtubules.

6 l all kinetochores are correctly attached to spindle microtubules.

7 ly of the subpellicular microtubules and the spindle microtubules.

8 sphorylated Aurora A could be at play in the spindle microtubules.

9 C) activation, with chromosome attachment to spindle microtubules.

10 r spindle lengths, along with poorly aligned spindle microtubules.

11 control the dynamics of specific subsets of spindle microtubules.

12 in a stereotypic cluster encircling central spindle microtubules.

13 5, truncated Sli15 localizes to pre-anaphase spindle microtubules.

14 d-bearing attachments to the dynamic tips of spindle microtubules.

15 es that establish end-coupled attachments to spindle microtubules.

16 ends on stable attachment of kinetochores to spindle microtubules.

17 f bi-oriented attachments of kinetochores to spindle microtubules.

18 interactions between chromosomes and dynamic spindle microtubules.

19 hat facilitates attachment of chromosomes to spindle microtubules.

20 omosomes and is required for the assembly of spindle microtubules.

21 moting robust kinetochore attachments to the spindle microtubules.

22 netochores stably attach to the plus ends of spindle microtubules.

23 osomes organize and nucleate the majority of spindle microtubules.

24 osome and functions to attach chromosomes to spindle microtubules.

25 correct attachments between chromosomes and spindle microtubules.

26 t signal transduction after interaction with spindle microtubules.

27 es are the chromosomal attachment points for spindle microtubules.

28 ) of K-fibers but no change in the t(1/2) of spindle microtubules.

29 that localizes to the outer kinetochore and spindle microtubules.

30 he dynamic interface between centromeres and spindle microtubules.

31 f chromosomes and the bipolar arrangement of spindle microtubules.

32 gamma-tubulin functions by binding weakly to spindle microtubules.

33 25 dimers, directly connects kinetochores to spindle microtubules.

34 med the kinetochore to attach chromosomes to spindle microtubules.

35 otile tether linking kinetochores to dynamic spindle microtubules.

36 h its N-terminal domain that hyperstabilizes spindle microtubules.

37 romosomes have formed bipolar attachments to spindle microtubules.

38 calized to spindle pole bodies but not along spindle microtubules.

39 ween kinetochores of mitotic chromosomes and spindle microtubules.

40 ls when kinetochores actively exert force on spindle microtubules.

41 mosomes remain juxtaposed on detachment from spindle microtubules.

42 that a fraction of C19ORF5 localizes to the spindle microtubules.

43 netochores must form stable attachments with spindle microtubules.

44 es by cross-linking and sliding antiparallel spindle microtubules.

45 omes have achieved bipolar attachment to the spindle microtubules.

46 the primary chromosomal attachment sites for spindle microtubules.

47 associated with developing spindle poles and spindle microtubules.

48 crotubule regulatory factors in proximity to spindle microtubules.

49 ing the correct attachment of chromosomes to spindle microtubules.

50 the mechanism by which kinetochores capture spindle microtubules.

51 aling through its capture at kinetochores of spindle microtubules.

52 e coordinated by the function of the dynamic spindle microtubules.

53 , kinetochores form dynamic connections with spindle microtubules.

54 regation by forming dynamic connections with spindle microtubules.

55 ch attach chromosomes to the dynamic tips of spindle microtubules.

56 that link chromosomal centromeres to mitotic-spindle microtubules.

57 ctly adjacent to NE holes containing meiotic spindle microtubules.

58 nce of kinetochores that are not attached to spindle microtubules.

59 , targets kinesin-5 and kinesin-12 motors to spindle microtubules.

60 onset until all chromosomes are attached to spindle microtubules.

61 single kinetochore is unattached to mitotic spindle microtubules.

62 til all chromosomes are properly attached to spindle microtubules.

63 erved machinery that connects chromosomes to spindle microtubules.

64 increase in the proportion of stable mitotic spindle microtubules.

65 ich regulates cortical attachments of astral spindle microtubules.

66 itate kinetochore movement on depolymerizing spindle microtubules.

67 rotein, plays a critical role in stabilizing spindle microtubules.

68 ed to AurkinA mislocalise AURKA from mitotic spindle microtubules.

69 t not the subpellicular microtubules and the spindle microtubules.

70 transition until all kinetochores attach to spindle microtubules [1, 2].

71 s a vital connection between chromosomes and spindle microtubules [1, 2].

72 ate interactions between chromosomal DNA and spindle microtubules [1].

73 ting kinetochore number alters the number of spindle microtubules: adding extra kinetochores increase

74 ecular motor Kar3-Cik1 can efficiently align spindle microtubules along the spindle axis.

75 cular interface that couples kinetochores to spindle microtubules also functions in neuronal developm

76 inetochore mediates chromosome attachment to spindle microtubules and acts as a scaffold for signalin

77 yeast, it is required for the dynamicity of spindle microtubules and also for kinetochore force gene

78 lomere separation occurred in the absence of spindle microtubules and an actin cytoskeleton and persi

79 Kinetochores link chromosomes to spindle microtubules and are essential for accurate chro

80 parate until all chromosomes are attached to spindle microtubules and bi-oriented.

81 king stronger kinetochores that capture more spindle microtubules and bias segregation.

82 by drug-induced depolymerization of mitotic spindle microtubules and by experimentally increasing th

83 on depends on a regulated connection between spindle microtubules and centromeric DNA.

84 in diverse spindles and suggest that central-spindle microtubules and chromosomes are strongly couple

85 which promote the proper interaction between spindle microtubules and chromosomes.

86 suggesting a strong coupling between central-spindle microtubules and chromosomes.

87 ex protein machines that link chromosomes to spindle microtubules and contain a structural core compo

88 meric chromatin to mediate interactions with spindle microtubules and control cell-cycle progression.

89 otic spindle but failed to recruit DYNLL1 to spindle microtubules and did not correct the spindle ori

90 The kinetochore links chromosomes to dynamic spindle microtubules and drives both chromosome congress

91 toring interactions between kinetochores and spindle microtubules and ensuring high-fidelity chromoso

92 activity is necessary for the positioning of spindle microtubules and has also been implicated in ina

93 some segregation by attaching chromosomes to spindle microtubules and integrating safeguard mechanism

94 The Dam1 complex attaches the kinetochore to spindle microtubules and is a processivity factor in vit

95 tosis through nucleation and organization of spindle microtubules and is regulated by kinesin-like pr

96 We find that Dam1 binds to the plus end of spindle microtubules and kinetochores as cells enter mit

97 Interfaces between spindle microtubules and kinetochores were examined in d

98 hromosomes is driven by interactions between spindle microtubules and kinetochores.

99 senses kinetochores not properly attached to spindle microtubules and prevents precocious sister-chro

100 mplexes that assemble on centromeres to bind spindle microtubules and promote faithful chromosome seg

101 ds preferentially to the most stable mitotic spindle microtubules and promotes their turnover.

102 ontaining ZNF207 (BuGZ) that associates with spindle microtubules and regulates chromosome alignment.

103 Kinetochores attach chromosomes to the spindle microtubules and signal the spindle assembly che

104 chromosomes must be efficiently captured by spindle microtubules and subsequently aligned on the mit

105 ation relies on a complex dialog between the spindle microtubules and the cell cortex, in which F-act

106 Dynamic interaction between spindle microtubules and the kinetochore complex that as

107 ural core, its components that interact with spindle microtubules and the spindle assembly checkpoint

108 s-end-directed motor localized to interpolar spindle microtubules and to the spindle poles.

109 ly to centrosomes and midbodies, but also to spindle microtubules and transiently to kinetochores.

110 in cells caused ubiquitous detyrosination of spindle microtubules, and CENP-E transported chromosomes

111 with polyglutamylated tubulin to centrioles, spindle microtubules, and cilia in human tissue culture

112 detect and signal the lack of attachment to spindle microtubules, and delay anaphase onset in respon

113 ion of the dynein complex from kinetochores, spindle microtubules, and spindle poles during mitosis.

114 cytoplasmic microtubules during interphase, spindle microtubules, and/or kinetochores during early m

115 rotein localizes to a specific population of spindle microtubules, appearing to be excluded from the

116 hich hKif15 and its inhibitor hTpx2 modulate spindle microtubule architecture.

117 on, the interactions between chromosomes and spindle microtubules are disturbed and can result in the

118 ulated interactions between kinetochores and spindle microtubules are essential to maintain genomic s

119 condary assays, led to new insights into how spindle microtubules are generated; how centrosomes are

120 ion is lost between sister kinetochores, and spindle microtubules are no longer resistant to low dose

121 Proper connections between centromeres and spindle microtubules are of critical importance in ensur

122 For example, spindle microtubules are organized by numerous cross-lin

123 In general, metaphase spindle microtubules are oriented such that their plus e

124 Dynamic attachments between kinetochores and spindle microtubules are required for chromosome bi-orie

125 protein levels and altered interactions with spindle microtubules, are preferentially retained in the

126 g to microtubules is required for organizing spindle microtubule arrays but not cargo motility in viv

127 w that centralspindlin travels along central spindle microtubules as higher-order clusters.

128 crucial role for Incenp in chromosome-driven spindle microtubule assembly in living oocytes.

129 While spindle microtubule assembly normally requires Dasra-dep

130 DYNLL1 interacted with a spindle-microtubule-associated adaptor formed by CHICA a

131 ecruitment to kinetochores fully attached to spindle microtubules at metaphase.

132 During cell division, chromosomes attach to spindle microtubules at sites called kinetochores, and f

133 s dynein helps to anchor a focused bundle of spindle microtubules at the pole.

134 localizations at spindle poles (AtAurora1), spindle microtubules (AtEDE1), phragmoplast microtubules

135 entromeres are the chromosomal loci at which spindle microtubules attach to mediate chromosome segreg

136 on the proper attachment of kinetochores to spindle microtubules before anaphase onset.

137 mperature indicated that MEI-1 was lost from spindle microtubules before loss of ASPM-1, indicating t

138 It is crucial for the formation of central spindle microtubule bundle structure.

139 he physical coupling of sister chromatids to spindle microtubule bundles (called kinetochore (K)-fibr

140 ation and contributed to Eg5 localization to spindle microtubules but not spindle poles.

141 ely stable attachment of all kinetochores to spindle microtubules, but not for the fidelity of the mi

142 n, kinetochores must be properly captured by spindle microtubules, but the mechanisms underlying init

143 , restrain mitotic exit when not attached to spindle microtubules by generating a Cdc20-containing co

144 Essential to this process are the capture of spindle microtubules by kinetochores and the congression

145 tributes to the capture and stabilization of spindle microtubules by kinetochores.

146 In these systems, damaging central-spindle microtubules by laser ablation caused an immedia

147 l. challenge this assumption by showing that spindle microtubules can effectively position the divisi

148 mitotic chromosomes in animals to accelerate spindle microtubule capture and to control spindle check

149 How the state of spindle microtubule capture at the kinetochore is transl

150 ent BubR1 autophosphorylation in response to spindle microtubule capture by CENP-E is important for k

151 int signaling that is not silenced either by spindle microtubule capture or the tension developed at

152 ed microtubule depolymerase KLP10A increased spindle microtubule density.

153 strates how kinetochore microtubules amplify spindle microtubule density.

154 bule turnover varies among different mitotic spindle microtubules, dictated by their spatial distribu

155 ive or absent, which would otherwise lead to spindle microtubule disorganization and aneuploidy.

156 00 protofilaments showed that all classes of spindle microtubules displayed some flaring protofilamen

157 ore forms the site of attachment for mitotic spindle microtubules driving chromosome segregation.

158 omain and are well described to organize the spindle microtubule during mitosis using an additional m

159 Stabilization of spindle microtubules during anaphase is essential for pr

160 chromosomes to properly segregate along the spindle microtubules during anaphase.

161 hat monitors proper chromosome attachment to spindle microtubules during cell division.

162 ultrastructural characterizations of central-spindle microtubules during chromosome segregation in di

163 port measurements of the movement of central-spindle microtubules during chromosome segregation in hu

164 netic information by coupling chromosomes to spindle microtubules during mitosis and meiosis.

165 and provides the major attachment point for spindle microtubules during mitosis.

166 ulating attachments between kinetochores and spindle microtubules during mitosis.

167 nt upon stable attachment of kinetochores to spindle microtubules during mitosis.

168 inetochores connect centromeric chromatin to spindle microtubules during mitosis.

169 tochores, macromolecular complexes that bind spindle microtubules during mitosis.

170 izes strongly to kinetochores and to central spindle microtubules during prometaphase and metaphase I

171 protein, to the plus ends of interdigitating spindle microtubules during the metaphase-to-anaphase tr

172 irs of proteins by comparing cytoplasmic and spindle microtubule dynamics in cells lacking either one

173 otubule-associated protein, thereby coupling spindle microtubule dynamics with kinetochore capture.

174 nhibits cell proliferation is by suppressing spindle microtubule dynamics.

175 During cell division, spindle microtubules ensure an equal repartition of chro

176 e proteins distinguish cortical from mitotic spindle microtubules, even though the assembly of these

177 ntrosomes and NuMA, initial establishment of spindle microtubule focusing completely fails.

178 some requires a centromere for attachment to spindle microtubules for chromosome segregation.

179 ociates with condensed chromosomes utilizing spindle microtubules for delivery.

180 chanism by which this complex interacts with spindle microtubules for timely and accurate chromosome

181 rovides the mechanistic basis, together with spindle microtubules, for generating tension between bio

182 constitute a molecular spring that balances spindle microtubule force in metaphase.

183 However, in interkinesis the central spindle microtubules from meiosis I persisted for a shor

184 CKAP2 associated with spindle poles and with spindle microtubules from prophase through anaphase and

185 otein which binds and recruits the latter to spindle microtubule in mitosis.

186 onset until all chromosomes are attached to spindle microtubules in a bipolar fashion [3, 4].

187 e show here that NUSAP1 localizes to dynamic spindle microtubules in a unique chromosome-centric patt

188 contributes to the localization of Kif15 to spindle microtubules in cells and suppresses motor walki

189 hat Tap42 mutations cause disorganization of spindle microtubules in larval neuroblasts, leading to a

190 helators cause immediate depolymerization of spindle microtubules in meiosis I and meiosis II.

191 ometric organization of DNA loops around the spindle microtubules in metaphase.

192 omosome and the kinetochore that connects to spindle microtubules in mitosis.

193 he primary binding site for the plus-ends of spindle microtubules in mitosis.

194 phase but exhibited reduced association with spindle microtubules in mitosis.

195 Our focus is primarily on the role of spindle microtubules in the development of the cerebral

196 RNAs incenp, xrhamm, and tpx2 associate with spindle microtubules in vivo.

197 chores of mitotic chromosomes are coupled to spindle microtubules in ways that allow the energy from

198 meiotic spindle, and a second affecting the spindle microtubules in which aPKCzeta plays a role.

199 , we demonstrate that complete disruption of spindle microtubules in Xenopus laevis oocytes did not a

200 titude of proteins that bind to and regulate spindle microtubules, including kinesin superfamily memb

201 ing dynein activity to minus-ends to cluster spindle microtubules into poles.

202 he poleward flux of tubulin subunits through spindle microtubules is a striking and conserved phenome

203 dynamic attachment between kinetochores and spindle microtubules is an essential prerequisite for ac

204 balance between kinesin motors on interpolar spindle microtubules is critical for correcting meroteli

205 The coupling of kinetochores to dynamic spindle microtubules is crucial for chromosome positioni

206 How turnover among the various classes of spindle microtubules is differentially regulated and the

207 First, the initial assembly of spindle microtubules is drastically delayed in an incenp

208 The interaction between chromosomes and spindle microtubules is essential for chromosome segrega

209 these motors for different subpopulations of spindle microtubules is not understood.

210 complex, which mediates end-on attachment of spindle microtubules, is linked to centromeric chromatin

211 omosome is transported to the minus end of a spindle microtubule, its kinetochore-bound microtubule p

212 that either generate sliding forces between spindle microtubules (Kinesin-5, Kinesin-14, dynein), pr

213 ard movement of tubulin subunits through the spindle microtubule lattice.

214 gated to daughter cells via interaction with spindle microtubules mediated by the kinetochore.

215 ng to kinesin-5 Cut7-dependent aberrant long spindle microtubule minus-end protrusions that push the

216 We know that dynein pulls on mammalian spindle microtubule minus-ends, and this localized activ

217 In dividing cells, depolymerizing spindle microtubules move chromosomes by pulling at thei

218 y aspects of mitotic chromosome behavior and spindle microtubule (MT) dynamics to ensure accurate chr

219 are linked to the active depolymerization of spindle microtubule (MT) ends.

220 he initial kinetochore (KT) encounter with a spindle microtubule (MT; KT capture) is one of the rate-

221 c DNA, bi-orient paired sister chromatids on spindle microtubules (MTs) and control cell-cycle progre

222 sembly, a multiprotein complex that attaches spindle microtubules (MTs) and is required for chromosom

223 nt of kinetochores (KTs) to the plus ends of spindle microtubules (MTs) and prevents anaphase onset u

224 itosis is required for bipolar attachment of spindle microtubules (MTs) and the accumulation of spind

225 pulling forces generated by distinct sets of spindle microtubules (MTs) and their interactions with m

226 maintains a constant length, even though the spindle microtubules (MTs) are constantly undergoing pol

227 und the nucleus may signal spindle assembly: spindle microtubules (MTs) are first assembled when the

228 attachments between kinetochores and dynamic spindle microtubules (MTs) are important for accurate ch

229 cular assemblies that connect chromosomes to spindle microtubules (MTs) during mitosis.

230 nd AurB results in a failure to depolymerize spindle microtubules (MTs) in anaphase after Cdk1 inacti

231 ubulin and is required for polymerization of spindle microtubules (MTs) in vivo.

232 le but dynamic links between chromosomes and spindle microtubules (MTs) is essential for accurate chr

233 The NEDD1 protein decorates spindle microtubules (MTs) preferentially toward spindle

234 Prometaphase kinetochores interact with spindle microtubules (MTs) to establish chromosome bi-or

235 binding, cross-linking, and sliding adjacent spindle microtubules (MTs), or, alternatively, it may bi

236 teraction with each other and recruitment to spindle microtubules (MTs).

237 omosomes segregate at the ends of shortening spindle microtubules (MTs).

238 regation relies on the dynamic properties of spindle microtubules (MTs).

239 udy, we analyzed the mechanisms that control spindle microtubule nucleation and stability in mammalia

240 t the poles and the equator, consistent with spindle microtubules of mixed polarity, differing from e

241 ssion by either sliding along the lattice of spindle microtubules or forming end-on attachments to th

242 hen individual kinetochores are not bound to spindle microtubules or not under tension.

243 Knockdown of Kif18B causes defects in spindle microtubule organization and a dramatic increase

244 Paclitaxel treatment caused a disruption in spindle microtubule organization marked by a significant

245 ed an increase in the number of disorganized spindle microtubules owing to multipolar configurations

246 ted with the presence of one or a few stable spindle microtubule plus ends at the cortex.

247 Kinetochore attachment to spindle microtubule plus-ends is necessary for accurate

248 of concept, we use ISI to measure metaphase spindle microtubule poleward flux in primary cells and e

249 ol/L in the absence of detectable effects on spindle microtubule polymer mass.

250 pport a model where Kip3 directly suppresses spindle microtubule polymerization, limiting midzone len

251 bipolar attachment of the kinetochore to the spindle microtubule remain largely elusive.

252 which different factors organize the central spindle microtubules remain unclear.

253 id cohesion in response to the disruption of spindle microtubules, show enhanced sensitivity to nocod

254 We found that these central-spindle microtubules slide apart at the same speed as ch

255 paclitaxel, versus specific perturbation of spindle microtubule subsets by MCAK inhibition.

256 g extra kinetochores increases the number of spindle microtubules, suggesting kinetochore-based regul

257 hores were frequently abnormally attached to spindle microtubules, suggesting that kinetochore fragme

258 eukaryote known), mitosis does involve fewer spindle microtubules than chromosomes.

259 Both mutants exhibit defective monopolar spindle microtubules that emanate from the mother SPB.

260 taining Aurora B can be found on a subset of spindle microtubules that exist near prometaphase kineto

261 vision, the mammalian kinetochore binds many spindle microtubules that make up the kinetochore-fiber.

262 specific posttranslational detyrosination of spindle microtubules that point to the equator.

263 otubules and targets condensation of LEM2 to spindle microtubules that traverse the nascent nuclear e

264 ng to detachment of selfish centromeres from spindle microtubules that would direct them to the polar

265 In addition, after attaching to spindle microtubules, the kinetochore generates the forc

266 connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interactin

267 Kif18A regulates spindle microtubules through its dual functionality, wit

268 nous calmodulin was found to colocalize with spindle microtubules throughout all stages of meiosis.

269 We find that cyclin B associates with spindle microtubules throughout meiosis I and meiosis II

270 arrests mitosis until bipolar attachment of spindle microtubules to all chromosomes is accomplished.

271 r the centrosomal protein Cep57 in attaching spindle microtubules to both kinetochores and centrosome

272 rganization relies on TPX2 as it cross-links spindle microtubules to centrosome-associated mitotic as

273 load-bearing attachments of the plus ends of spindle microtubules to chromosome structures named kine

274 mble at the interface with centromeres, bind spindle microtubules to ensure faithful delivery of chro

275 cluding 1) connecting mitotic chromosomes to spindle microtubules to establish force-transducing kine

276 In fission yeast, erroneous attachments of spindle microtubules to kinetochores are frequent in ear

277 checkpoint, that monitors the attachment of spindle microtubules to kinetochores as a means of detec

278 o the metaphase plate and to the coupling of spindle microtubules to kinetochores during anaphase A.

279 ation requires stable bipolar attachments of spindle microtubules to kinetochores.

280 chores, multi-subunit complexes that capture spindle microtubules to promote chromosome segregation d

281 multiprotein complex that interacts with the spindle microtubules to promote proper chromosomal align

282 Our data suggest that EB1 enables the spindle microtubules to regulate the phosphorylation of

283 B component Spc110p connects the ends of the spindle microtubules to the core of the SPB.

284 The SAC monitors proper attachment of spindle microtubules to the kinetochore formed at each c

285 Creating stable yet flexible attachments of spindle microtubules to the kinetochore is critical for

286 segregation in mitosis requires tethering of spindle microtubules to the kinetochore.

287 icentric chromatin and cohesin that encircle spindle microtubules undergo a radial expansion.

288 accomplished by attachment of chromosomes to spindle microtubules using the kinetochore.

289 aphase plate maintain dynamic attachments to spindle microtubules via their kinetochores, and multipl

290 Using laser ablation to cut spindle microtubules, we identify a force that rapidly a

291 icrotubule attachments were impaired because spindle microtubules were unstable in response to cold t

292 cannot make subsequent stable attachment to spindle microtubules when dephosphorylation of CENP-E or

293 anaphase, it began to migrate to the central spindle microtubules, where it remained through telophas

294 ADIN, Aurora A spreads from centrosomes onto spindle microtubules, which affects the distribution of

295 ding forces within the midzone cannot buckle spindle microtubules, which allows the cell boundary to

296 bly checkpoint monitors the integrity of the spindle microtubules, which attach to sister chromatids

297 tachment between the kinetochore and dynamic spindle microtubules, which undergo cycles of polymeriza

298 Spindle microtubules, whose dynamics vary over time and

299 hibition caused a dramatic reorganization of spindle microtubules with a significant increase in astr

300 defective in proper end-on attachment to the spindle microtubule, with chromosome alignment defects a