ライフサイエンスコーパス: spliced leader

1 ) conveyed via trans-splicing of a universal spliced leader.

2 -5 mRNAs are themselves trans-spliced to SL1 spliced leaders.

3 n-1 RNA transcripts are trans-spliced to the spliced leader 1 and undergo alternative splicing to cod

4 pression of histone, piRNA, and 5S rDNA-SL1 (spliced leader 1) clusters.

5 The elements specifically insert in the Spliced leader-1 genes; hence the element has been named

6 the element has been named NeSL-1 (Nematode Spliced Leader-1).

7 TbMTr1 favors the spliced leader 5' sequence, as reflected by a preference

8 A longer spliced leader also can be tolerated but only when prese

9 ng replaces the 5' end of pre-mRNAs with the spliced leader, an exon derived from a specialised non-c

10 modifies the first transcribed nucleotide of spliced leader and U1 small nuclear RNAs in the kinetopl

11 ions in the otherwise highly conserved 22-nt spliced leader are tolerated for splicing and post-splic

12 ties and, meanwhile, demonstrate that unique spliced leaders are useful for profiling lineage-specifi

13 g metazoan groups (e.g. nematodes), flatworm spliced leaders are variable in both sequence and length

14 rypanosomes, the expression site body (ESB), spliced leader array body (SLAB), NUFIP body and Cajal b

15 ntly discovered dinoflagellate mRNA-specific spliced leader as a selective primer, we constructed cDN

16 rst characterized in Trypanosoma brucei, the spliced leader-associated (SLA) RNA gene locus has now b

17 SL RNA allowed us previously to identify the spliced leader-associated (SLA) RNA.

18 e-specific non-LTR retrotransposon SLACS, or spliced leader-associated conserved sequence, which inte

19 elomere-associated transposable elements and spliced leader-associated SLACS retrotransposons.

20 RNA contains an identical 39-nucleotide (nt) spliced leader at its 5'-terminus.

21 tor methionine context and the effect of the spliced leader AUG added upstream and out-of-frame with

22 ously suggested that the Schistosoma mansoni spliced leader AUG might contribute a required translati

23 For the nt 28-39 mutated spliced leaders, both the substrate spliced leader RNA a

24 r RNA has the same first four nucleotides as spliced leader, but it receives an m(2,2,7)G cap with hy

25 s is the first report demonstrating that the spliced leader contains critical structural or sequence

26 oth the substrate spliced leader RNA and the spliced leader demonstrated a wild-type methylation patt

27 mutations spanning nucleotides 10-39 of the spliced leader did not affect substrate spliced leader R

28 Undermethylated spliced leaders did not associate efficiently with polys

29 The target specificity of NeSL-1 for the spliced leader exons and the similarity of its structure

30 ubstrate spliced leader RNA and the nt 10-19 spliced leaders found in the poly(A)+ population of RNA;

31 for the cap 4 and/or primary sequence of the spliced leader in translation.

32 structure was present on substrate and mRNA spliced leaders in nt 20-29 mutated exons; nt 20-29 mRNA

33 he 5' end of mRNAs by a m(7)G cap-containing spliced leader is a developing theme in the lower eukary

34 The spliced leader is derived from substrate spliced leader

35 Spliced leader is the majority RNA polymerase II transcr

36 conserved and unique feature of all flatworm spliced leaders is the presence of a 3'-terminal AUG.

37 In trans-splicing, a common exon, the spliced leader, is added to all mRNAs from a small RNA.

38 function of SL1, the major C. elegans trans-spliced leader, is unknown, SL1 RNA, which contains this

39 Interestingly, the spliced leader itself can be dramatically altered, such

40 site on a small RNA of uniform sequence (the spliced leader or SL RNA) has allowed us to characterize

41 rtional to the tagged:wild type ratio in the spliced leader precursor population.

42 This multiplex assay is targeted to the spliced leader RNA (mini-exon) gene repeats of these org

43 The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorh

44 Recombinant TbCgm1 transfers the GMP to spliced leader RNA (SL RNA) via a covalent enzyme-GMP in

45 We report that in Leishmania tarentolae spliced leader RNA 5' modification is influenced by the

46 How the spliced leader RNA and its target transcripts are brough

47 The spliced leader is derived from substrate spliced leader RNA and joined to pre-mRNA by trans-splic

48 tion was seen between the nt 10-19 substrate spliced leader RNA and the nt 10-19 spliced leaders foun

49 mutated spliced leaders, both the substrate spliced leader RNA and the spliced leader demonstrated a

50 ls for the 5' half of Caenorhabditis elegans spliced leader RNA by comparison of the two-dimensional

51 The spliced leader RNA gene (SL RNA) repeat is present in la

52 sion in these protists, we have investigated spliced leader RNA gene transcription.

53 sable for RNA polymerase II transcription of spliced leader RNA genes (SLRNAs).

54 acidic repetitive genes, Pol II-transcribed spliced leader RNA genes, and Pol III-transcribed U-snRN

55 In Leishmania tarentolae the precursor spliced leader RNA is 96 nucleotides, with a 39-nucleoti

56 A pathway for Leishmania spliced leader RNA processing and maturation is proposed

57 Regulation of spliced leader RNA synthesis is controlled by a triparti

58 Spliced leader RNA transcription is essential for cell v

59 the spliced leader did not affect substrate spliced leader RNA transcription or trans-splicing in Le

60 The precursor spliced leader RNA was tested for trans-splicing functio

61 es, which encode the trans splicing-specific spliced leader RNA, suggests that trypanosomatids assemb

62 stent with its essential role in the Ascaris spliced leader RNA, whereas in Leptomonas mutation of th

63 es and nematodes has been characterized as a spliced leader RNA-facilitated reaction; in contrast, it

64 tes within the first four nucleosides on the spliced leader RNA.

65 a 39-nucleotide-long sequence donated by the spliced leader RNA.

66 sms that have a trans-splicing reaction from spliced leader RNA.

67 ared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a

68 is and T. muris operons are trans-spliced to spliced leader RNAs, and we are able to detect polycistr

69 ion, whereas addition of either a TMG-cap or spliced leader sequence alone decreased reporter activit

70 action and an oligonucleotide comprising the spliced leader sequence in the second strand reaction.

71 The trans-spliced leader sequence on mRNAs reduces Dcp2 activity a

72 hat BmGMF is trans-spliced with the nematode spliced leader sequence SL1 and is expressed in microfil

73 lation system, we found that the TMG-cap and spliced leader sequence synergistically collaborate to p

74 e SL1 RNA promoter partly resides within the spliced leader sequence.

75 anscripts, including splice junctions, trans-spliced leader sequences, and polyadenylation tracts.

76 luding occurrence and length distribution of spliced leader sequences, the functional landscape of en

77 We analyzed parasite mini-exon spliced-leader sequences following amplification of bloo

78 everal other lower eukaryotic phyla involves spliced leader (SL) addition trans-splicing.

79 Addition of a 39-nucleotide (nt) spliced leader (SL) by trans splicing is a basic require

80 element responsible for transcription of the spliced leader (SL) gene of Trypanosoma cruzi was identi

81 Through trans-splicing of a 39-nt spliced leader (SL) onto each protein-coding transcript,

82 rasite Trypanosoma brucei, the small nuclear spliced leader (SL) RNA and the large rRNAs are key mole

83 ypanosomes, the m7G cap is restricted to the spliced leader (SL) RNA and the precursors of U2, U3, an

84 scription preinitiation complex (PIC) at the spliced leader (SL) RNA gene (SLRNA) promoter.

85 d, transgenic worms are generated in which a spliced leader (SL) RNA gene is fused with a sequence ta

86 uence, which integrates exclusively into the spliced leader (SL) RNA genes.

87 We report the first data that the spliced leader (SL) RNA is a more specific marker for cu

88 n all trypanosomatids, trans splicing of the spliced leader (SL) RNA is a required step in the matura

89 Spliced leader (SL) RNA trans splicing adds a trimethylg

90 Spliced leader (SL) RNA trans-splicing contributes the 5

91 ting RNA polymerase II-mediated synthesis of spliced leader (SL) RNA, a trans splicing substrate and

92 ction of mRNA relies on the synthesis of the spliced leader (SL) RNA.

93 The two spliced leader (SL) RNAs (SL-A and -B) contain splice do

94 Both the cap structure and the spliced leader (SL) sequence affect levels of A. suum eI

95 transcriptional mechanism whereby a specific Spliced Leader (SL) sequence is added to the 5'end of ea

96 ediated through trans-splicing of the capped spliced leader (SL) sequence of the SL RNA onto the 5' e

97 This short 39 nt m(7)G-capped RNA, the spliced leader (SL) sequence, is expressed as an approxi

98 ypanosomes involves the addition of a common spliced leader (SL) sequence, which is derived from a sm

99 kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic precursors conveys

100 served that CRK9 silencing led to a block of spliced leader (SL) trans splicing, an essential step in

101 In trypanosomes, mRNAs are processed by spliced leader (SL) trans splicing, in which a capped SL

102 Metazoan spliced leader (SL) trans-splicing generates mRNAs with

103 Spliced leader (SL) trans-splicing in Caenorhabditis ele

104 Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods.

105 Spliced leader (SL) trans-splicing is a biological pheno

106 Spliced leader (SL) trans-splicing is a critical element

107 Spliced leader (SL) trans-splicing replaces the 5' end o

108 toplastid flagellates attach a 39-nucleotide spliced leader (SL) upstream of protein-coding regions i

109 ced with the addition of the 22-nt conserved spliced leader (SL), DCCGUAGCCAUUUUGGCUCAAG (D = U, A, o

110 mpanied by trans-splicing with a specialized spliced leader (SL), SL2.

111 In T. brucei, spliced leader (SL)-mediated trans-splicing of pre-mRNAs

112 All trypanosome mRNAs have a spliced leader (SL).

113 The role of spliced leaders (SL) within the cell is unclear and an a

114 Trypanosoma cruzi, and Leishmania spp., the spliced-leader (SL) RNA is a key molecule in gene expres

115 ide RNA derived from a short transcript, the spliced-leader (SL) RNA.

116 Ten different spliced-leader (SL) sequences were found attached to T20

117 Our data suggest that operons and "spliced leader" (SL) trans-splicing predate the radiatio

118 teract with components of the major nematode spliced leader (SL1) snRNP.

119 Of the two spliced leaders, SL1 is trans spliced to the 5' ends of

120 trans splicing, generally to the specialized spliced leader SL2, at the 5' ends of the downstream gen

121 products are trans spliced to a specialized spliced leader, SL2.

122 ciated with the Caenorhabditis elegans major spliced leader snRNP (SL1 snRNP), which donates the spli

123 leader snRNP (SL1 snRNP), which donates the spliced leader that replaces the 5' untranslated region

124 While introns are rare in these organisms, spliced leader trans splicing is an obligatory step in m

125 ins must deal with two populations of mRNAs, spliced leader trans-spliced mRNAs with a trimethylguano

126 Spliced leader trans-splicing (SLTS) plays a part in the

127 cally, and individual mRNAs are generated by spliced leader trans-splicing and polyadenylation, proce

128 Spliced leader trans-splicing is an mRNA maturation proc

129 Spliced leader trans-splicing is essential for gene expr

130 d evidence that another function of flatworm spliced leader trans-splicing is to provide some recipie

131 Spliced leader trans-splicing of pre-mRNAs is a critical

132 al for nematode viability and is involved in spliced leader trans-splicing.

133 methylguanosine (m(2,2,7)G) cap derived from spliced leader trans-splicing.

134 ng adds a capped 39-nucleotide mini-exon, or spliced leader transcript, to the 5' end of the main cod

135 Because deg-3 cDNAs contain the SL2 trans-spliced leader, we suggested that deg-3 was transcribed

136 association of mRNAs, which possess mutated spliced leaders, with polysomes.