ライフサイエンスコーパス: splicing endonuclease

1 to the functional assembly of the human tRNA splicing endonuclease.

2 al of introns catalyzed in yeast by the tRNA splicing endonuclease.

3 ation and characterization of the human tRNA splicing endonuclease.

4 ircularization site is processed by the tRNA splicing endonuclease.

5 ty from that of previously characterized RNA splicing endonucleases.

6 Three strictly conserved residues of the splicing endonuclease, a histidine, a lysine, and a tyro

7 ition and cleavage, is performed by the tRNA-splicing endonuclease, a tetrameric enzyme composed of t

8 l BHB constructs showed that the N. equitans splicing endonuclease accepts a broader range of substra

9 ite of the enzyme is similar to that of tRNA splicing endonucleases, and concordantly, Cas6 activity

10 lytically active ribonucleoprotein (RNP) has splicing, endonuclease, and reverse transcriptase activi

11 Here, we show that the N. equitans splicing endonuclease cleaves tRNA precursors containing

12 The RNA splicing endonuclease cleaves two phosphodiester bonds w

13 re performed on the RNA structures both in a splicing endonuclease complex and in the aqueous solutio

14 1 is associated with the transfer RNA (tRNA) splicing endonuclease complex and the cleavage and polya

15 TSEN54, which encodes a subunit of the tRNA splicing endonuclease complex.

16 N. equitans splicing endonuclease comprises the catalytic subunit (N

17 r results demonstrate that the eucaryal tRNA splicing endonuclease contains two functionally independ

18 d a similar enzyme assembly, currently known splicing endonuclease families have limited RNA specific

19 hallmark structure required by the archaeal splicing endonuclease for recognition and excision of al

20 olution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound w

21 The splicing endonuclease from Archaeoglobus fulgidus (AF) b

22 rized splicing endonucleases in Archaea, the splicing endonuclease from archaeum Sulfolobus solfatari

23 he previously determined homotetrameric tRNA splicing endonuclease from Methanococcus jannaschii (MJ)

24 The recently identified splicing endonuclease homolog from Sulfolobus solfataric

25 Different from previously characterized splicing endonucleases in Archaea, the splicing endonucl

26 The tRNA splicing endonuclease is a highly evolutionarily conserv

27 uggest an intriguing hypothesis in which the splicing endonuclease is an intermediate in the transiti

28 In yeast, the tRNA splicing endonuclease is responsible for identification

29 The RNA splicing endonuclease is responsible for recognition and

30 found in the gene encoding the archaeal tRNA splicing endonuclease of H. volcanii and in other Archae

31 ly shown that a defect in the essential tRNA splicing endonuclease of yeast results in transcriptome

32 -157), the first for a subunit of a eukaryal splicing endonuclease, revealed that the protein possess

33 tured known 5'-OH fragments produced by tRNA Splicing Endonuclease (SEN) during processing of intron-

34 from pre-tRNA is a heterotetrameric complex (splicing endonuclease [SEN] complex).

35 We demonstrate here that an inactive RNA splicing endonuclease subunit can be switched "on" solel

36 ort the solution structure of the human tRNA splicing endonuclease subunit HsSen15.

37 recently identified (alphabeta)(2) family of splicing endonucleases that require two different subuni

38 catalysis of pre-tRNA by the eukaryotic tRNA-splicing endonuclease therefore requires a previously un

39 us (AF) belongs to the homodimeric family of splicing endonucleases, thought to have evolved from the

40 tRNA precursors involves cleavage by a tRNA splicing endonuclease to yield tRNA 3'-halves beginning

41 ecursors (pre-tRNAs) are excised by the tRNA splicing endonuclease TSEN in complex with the RNA kinas

42 to a loss of CLP1 interaction with the tRNA splicing endonuclease (TSEN) complex, largely reduced pr

43 tRNA intron removal is catalyzed by the tRNA splicing endonuclease (TSEN) complex.

44 The transfer RNA (tRNA) splicing endonuclease (TSEN) has been studied for its ab

45 Through its role in intron cleavage, tRNA splicing endonuclease (TSEN) plays a critical function i

46 requires the action of the heterotetrameric splicing endonuclease, which is composed of two catalyti

47 makes it well-suited for attack by archaeal splicing endonucleases, which are symmetric dimers.

48 d two avatar (av) or model pre-tRNAs and two splicing endonucleases with distinct mechanisms of recog