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1 icroscopy coupled to fluorescence and immuno-spotting.
2 n was achieved by using a robotic system for spotting.
3 iotinylated ligands than is required for pin spotting.
4 assays, most approaches use pins for contact spotting.
5 spectively, using the polypropylene tips for spotting.
6 the surface and the size of the tip used for spotting.
7 he steel tips of drafting pens were used for spotting.
8 better than that obtained with manual pipet spotting.
9 epending on the length of dsDNA) after dsDNA spotting.
14 By virtue of using a slide for multi-samples spotting and a flatbed scanner for signal-read out acqui
16 o a DNA microarray design showing successful spotting and hybridization on whole microarray slides.
21 pe consisting of variable amelanotic ventral spotting and reduced pigmentation on the extremities (ta
24 eparate protein expression, purification and spotting, and also overcomes the problem of long-term fu
25 g this automation suite, sample preparation, spotting, and analysis of 100 samples ranging from 12 to
26 elf-reported bleeding patterns, incidence of spotting, and associations with reproductive hormones am
27 tivity, abnormal gait, deafness, white belly spotting, and hypoplasia of Mullerian duct derivatives.
28 An array of droplet pairs was created by spotting aqueous droplets, dispersed in a lipid oil solu
30 ve incorporated a matrix-diluting and liquid-spotting automated work station and a DE MALDI mass spec
31 tochemicals in plant extract samples using a spotting automatic system, mobile phone camera and a com
32 usly we showed that mDANs generated by this 'spotting'-based method exhibit gene expression and (elec
33 rotocol could also be readily adapted to use spotting-based culture to further optimize the different
35 r detection of blood groups was developed by spotting blood to antibodies absorbed to paper and elute
36 ropyl) dimethylammonia]-1-propane sulfonate) spotting buffer, 24-h postspotting reaction at 100% rela
37 examined included oligo concentration in the spotting buffer, composition of the spotting buffer, pos
38 n in the spotting buffer, composition of the spotting buffer, postspotting "curing" conditions, and p
39 ogressive piebaldism, exhibit dominant white spotting but show no evidence of progressive depigmentat
40 rvival of these cells in vivo, because white spotting (c-kit(W/W)) mice, carrying a natural inactivat
42 00 pg/mL of dibiotinylated NPY and 0.1 mg/mL spotting capture antibody were found to offer the best p
43 ackground binding of proteins is overcome by spotting carbohydrates through a protein-resistant overl
44 f fabricating oligonucleotide microarrays by spotting commercial presynthesized 65-mers with 5' amine
46 ncement was found to be dependent on the DNA spotting concentration: below approximately 12.5 muM, ME
48 ked immunosorbent assay (ELISA) was based on spotting different antibodies in a circular pattern at t
49 luding vaginal pain/discomfort (44% vs 68%), spotting, discharge, and lower urinary tract symptoms.
51 by changing the lipid oil composition after spotting donor droplets, we were able to create asymmetr
52 ccessful our improved method is in precisely spotting drivers, thereby providing a useful instrument
53 ying the piebald mutation exhibit white coat spotting due to the regional absence of neural crest-der
54 sue, a pseudoinfection model was prepared by spotting Escherichia coli lipid extracts on mouse brain
58 rgeting high-cost Medicare patients (ie, hot spotting) for cost containment efforts would be a potent
59 paration greatly speeds processing while the spotting function allows for precise sample placement on
60 susceptibility gene 2 (Fv2), dominant white spotting gene (W), and Steel gene (Sl), regulate the ini
61 g ligands using conventional micro-array pin spotting have hindered the application of surface plasmo
62 e have used the varied expressivity of white spotting (hypopigmentation) observed in intrasubspecific
63 emical evidence that hydrocephalus and white spotting in B3glct mutants resulted from loss of ADAMTS2
64 ralized hypopigmentation and localized white-spotting in mice, with a lack of pigment on the belly, t
65 ing previous observations that the degree of spotting in piebald mice is dependent on the amount of E
66 tion, suggesting that the color dilution and spotting in vivo may result from reduced numbers of mela
68 The transgene has no effect on coat color spotting, indicating the critical time for EDNRB express
69 ntly in human piebaldism and mouse "dominant spotting." Interestingly, all of the 14 known missense m
70 ication of high-cost patients, known as "hot spotting," is well developed in medical populations, but
72 occurring null mutation of the EDNRB gene in spotting lethal (sl) rats, which exhibit aganglionic meg
75 ting mice with mutations affecting the white spotting locus (which encodes KIT) or the steel locus (w
76 a naturally occurring mutation in the white spotting locus that causes reduced c-Kit receptor expres
78 trix can also be applied using our system by spotting matrix droplets on the microarray in a separate
79 Adamts20 mutations cause the mouse white-spotting mutant belted (bt), whereas Adamts9 is essentia
80 ibrary of S. enterica serovar Typhimurium by spotting mutants on soft agar plates seeded with bacteri
81 (mnlt), a second hypopigmentation and white-spotting mutation identified on the C57BL/6J background,
82 d Ls/Ls mice (mice homozygous for the lethal spotting mutation that develop fetal megacolon after aga
83 nt cell development that are associated with spotting mutations in mice include members of several st
85 d-to-end SW(eq) accuracy for a small keyword-spotting network and near-SW(eq) accuracy on the much la
88 (300 microm in diameter) allows high-density spotting of lentivirus, permitting thousands of distinct
89 for genotyping by SPC-SBE and allows direct spotting of sample for rapid analysis by matrix-assisted
90 ing a click reaction after precise nanolitre spotting of specific hexynyl-terminated DNA capture prob
91 ptions of a dilution of coat color and white spotting of the belly and extremities, suggesting a deve
94 DNA microarrays produced by deposition (or 'spotting')of a single long oligonucleotide probe for eac
95 TOF MS system, including density of organism spotting on a stainless steel target plate and the direc
100 rt that a point mutation, the dominant white spotting oncogene allele, Kit(W-42J), exacerbates XY(DOM
103 urine leakage on filter paper (4-fold higher spotting, P<0.01; 2.5-fold higher urine area percentage,
104 Defined primary symptoms were postmenstrual spotting, pain during uterine bleeding, technical issues
109 Symptoms related to HRT (breast tenderness, spotting, pelvic discomfort, and mood changes) were mild
110 tured microarray under optimized conditions (spotting pH buffer, surface chemistry, blocking procedur
111 nsgene induction in skin resulted in a white spotting phenotype due to somatic ORFeus-mediated mutati
114 ytes are segregated from analytes during the spotting process by the action of the SALDI-chip during
117 that the microfluidic printer is capable of spotting proteins that are dilute (<0.1 microg/ml) and c
119 have both decreased, as has the incidence of spotting, relative to the source populations in Africa.
120 we spotted 1 muL of a 10 mM solution using a spotting robot and performed our experiments with a Bruk
121 hocyanin expression (C or G) and anthocyanin spotting (Rs)] were linked to RAPD loci, but only six of
122 with mendelian inheritance: the major white spotting (S) locus and the hair ridge in Rhodesian ridge
124 (ii) the battery of measures explored allows spotting strengths in methods that may not be 'best' ove
125 rays, DNA microarrays were also developed by spotting streptavidin-biotinylated oligonucleotide conju
126 ensor chips using copoly(DMA-NAS-MAPS) and a spotting technique, as well as the specificity of their
128 reduced compared to standard continuous flow spotting technologies as well as in comparison to a conv
129 a modification to our continuous-flow micro-spotting technology that improves ligand deposition.
131 ipids from a kinase assay can be isolated by spotting the crude reaction on a nitrocellulose membrane
134 can be clearly observed by the naked eye by spotting the resulting sensor solution onto an alumina T
137 d to dark purple or blue is enhanced through spotting the solution onto a C18 reversed-phase thin-lay
140 n technique recognition, boost confidence in spotting these techniques, increase people's ability to
141 neration characterized clinically by retinal spotting throughout the fundus and late onset retinal de
142 g electrospotting both the gold chip and the spotting tip are electrically connected to a potentiosta
144 e chloride: citric acid can prevent the mold spotting up to 29 days longer in comparison to bread wra
145 cancer risk using germ cell-deficient white-spotting variant (Wv) mice, incorporating oncogenic muta
148 Mice carrying certain mutations in the white spotting (W) locus (ie, c-kit) exhibit reduced c-kit tyr
149 Finally, mice having mutations in the white spotting (W) locus, which encodes the SCF-receptor (c-ki
150 mouse strains, Steel (Sl) and dominant white spotting (W), to determine if stem cells from an inferti
153 he incidence of breakthrough bleeding and/or spotting was significantly higher only in the first 2 cy
155 eral amplification of BAC clone DNA prior to spotting, we designed a set of three novel degenerate ol
156 n is observed only within regions of retinal spotting, which is seen predominantly in the inferior na