ライフサイエンスコーパス: standard curve

1 well as provide novel evidence of a "FXS IQ standard curve".

2 e of the amplification profile to generate a standard curve.

3 gene usually by relating the PCR signal to a standard curve.

4 .5--> 527.5 transition of IS to generate the standard curve.

5 hence the isoform ratio by reference to the standard curve.

6 andard curve methods into a multidimensional standard curve.

7 o be 33 and 39% ON, respectively, by using a standard curve.

8 lculated by the 2( CT) method, and against a standard curve.

9 tration is determined without the need for a standard curve.

10 three SIL amino acids to provide an internal standard curve.

11 for researchers to create a sample-specific standard curve.

12 y reproducing the colorimetry-obtained ELISA standard curve.

13 red and non-linear, resulting in an unusable standard curve.

14 ted to quantify sample concentration using a standard curve.

15 unknown samples could be extrapolated from a standard curve.

16 plification are correlated by an exponential standard curve.

17 NA dilutions were qPCR-amplified to obtain a standard curve.

18 entration was calculated from an appropriate standard curve.

19 is time frame also improved linearity of the standard curve.

20 d analyte, as an internal standard against a standard curve.

21 itation can be achieved without reference to standard curves.

22 of each protein isoform by reference to the standard curves.

23 -739), and IS peptides were used to generate standard curves.

24 results may be obtained without the need for standard curves.

25 to interpolate the symmetric and asymmetric standard curves.

26 ked-in proteins of known amounts to generate standard curves.

27 hich led to a desired transition pH based on standard curves.

28 specific RPL19 primers were used to generate standard curves.

29 ful for quantification based on promastigote standard curves.

30 es were converted to concentrations by using standard curves.

31 method development and generation of add-in standard-curves.

32 encies of plasmid and cellular environmental standard curves (98 and 100%, respectively) allowed obta

33 the assay and enable quantification without standard curves, after initial characterization of the p

34 A standard curve, and (iv) external (13)C PA standard curve, all agreed within 1 digit in the same or

35 d on back-calculated adduct masses from five standard curves analyzed over a four-week period.

36 In a set of experiments in which the standard curve and algorithm were used to analyze and qu

37 antiserum shifted the double antibody assay standard curve and altered estimates of assay specificit

38 al relative quantification using an internal standard curve and need for calibrant diluent, and takes

39 etection, we were able to generate an in-gel standard curve and quantitate total disulfide contents w

40 ential decay equation best fit the universal standard curve and strain-specific curves (R2 of 0.96 an

41 he equation of the line of best fit for each standard curve and uses this equation to calculate compo

42 Pork DNA standard curves and cycle threshold (Ct) values were use

43 oeluted peaks, (3) absolute quantitation via standard curves and/or internal standards, (4) visualize

44 ope one-point calibration, (iii) external PA standard curve, and (iv) external (13)C PA standard curv

45 t methods (absolute standard curve, relative standard curve, and comparative C(T)) and show them to b

46 he universal standard curve, each subgroup's standard curve, and strain-specific curves were +/-0.87,

47 ally adequate sample numbers, an appropriate standard curve, and the inherent quantitative capacity o

48 Herein, we introduce SCALiR (standard curve application for determining linear ranges

49 rect assay can be corrected for by using the standard curve appropriately.

50 Standard curves are linear over a 10(3)-fold concentrati

51 Standard curves are linear over a 100-fold concentration

52 Linear standard curves are reported for a variety of compounds

53 Herein, we construct a standard curve based on representative solvation structu

54 h fiber and by comparing results to a single standard curve based on toxin in buffer.

55 Standard curves based on tenfold diluted plasmid standar

56 the number of GMOs has increased over time, standard-curve based simplex quantitative polymerase cha

57 on gene abundance and compared them with the standard curve-based method (the mixed model).

58 Standard curves between 0.04 and 10.00 nmol curcumin wer

59 We demonstrate that the standard curve can be used to determine the mean size of

60 s been extended to include a large number of standard curve classes and to use benchmark dose modelin

61 1% and accuracy between 93.8 and 107% at the standard curve concentration range.

62 from the sample absorbance and the reference standard curve constructed from the reference concentrat

63 d densitometry and threshold cycle data, the standard curve constructed from threshold cycle data had

64 3'TAMARA-labeled probe in comparison with a standard curve constructed with known copy numbers of it

65 Standard curves could be generated using absolute intens

66 measured by this method was 20 nM, with the standard curve covering a wide range (7.8-32,000 nM).

67 and recipient colonies were estimated from a standard curve created by LEW and BN bone marrow mixture

68 lied to the fillets were estimated using the standard curve data obtained from the correlation values

69 Results were quantitated by using a standard curve derived from a plasmid containing IS900.

70 abinitol concentrations were calculated from standard curves derived from pooled human serum containi

71 rtainty is a result of the highly uncertain "standard curve" developed during each test and (2) the u

72 for log(10) estimations using the universal standard curve, each subgroup's standard curve, and stra

73 yonic stem cell RNA) and measured associated standard curves, efficiency (57%), repeatability (~1 cyc

74 by comparing the mass signal integrals to a standard curve established using purified recombinant PS

75 This algorithm estimates standard curve features as well as nucleic acid concentr

76 The standard curve fitted linearly (R(2) = 0.9982) and allow

77 paper, a method for estimating n and Kd from standard curve-fitting procedures is established.

78 A standard curve for detection of cortisol in saliva was g

79 rted for DNA methylation, but they require a standard curve for quantification or only show moderate

80 The standard curve for the drug pazopanib was falsified to m

81 th purified allergens as reference standards.Standard curves for 17 allergens covered a 5-log dynamic

82 ed DENV and CHIKV RNAs were used to generate standard curves for absolute quantification.

83 Standard curves for all the sugars are established in a

84 photoluminescence microwell imager, and the standard curves for each analyte were quantified from th

85 The standard curves for each chondroitin disaccharide showed

86 Standard curves for each isoform demonstrated good sensi

87 Standard curves for nanomolar concentrations of ADP, UDP

88 led standard peptides, to construct internal standard curves for peptides derived from key nodes in s

89 PCR is sensitive to inhibitors and relies on standard curves for quantification, it has limited appli

90 in RNA-seq experiments as well as to derive standard curves for quantifying the abundance of transcr

91 Standard curves for S1P and DHS1P are linear over wide r

92 r absolute quantification when compared to a standard curve from the same Leishmania species.

93 Standard curves from 50 to 10,000 ng/mL are generated.

94 d ([(13)C(3)]malonyl-CoA) and multiple-point standard curves from 50 to 1000pmol.

95 ion that automatically generates calibration standard curves from series of standards that can be use

96 All standard curves generate by this method had coefficients

97 of the genome copies is extrapolated from a standard curve generated from amplification of quantifie

98 applied to the tissue was determined using a standard curve generated from MALDI time-of-flight (TOF)

99 The standard curve generated from the chip consisted of two

100 the SSB yields per DNA molecule, employing a standard curve generated using DNA molecules containing

101 ns from environmental samples using absolute standard curves generated by real-time qPCR.

102 Standard curves generated for each metabolite have corre

103 Computing metabolite concentrations using standard curves generated from standard mixtures of know

104 Standard curves generated suggested good linear relation

105 Even standard curves generated using free 7-amino-4-methylcou

106 molar concentrations, were interpolated from standard curves generated with synthetic peptides that c

107 ract can be obtained in less than 2 h once a standard curve has been prepared for H4PteGlun.

108 including absolute quantification without a standard curve, improved precision, improved accuracy in

109 ble simultaneous construction of an internal standard curve in the MS(1) precursor scan, real-time id

110 , underscore the importance of preparing the standard curve in the same matrix as the unknown sample

111 t this framework expands the capabilities of standard curves in order to optimize quantification perf

112 The authors suggest against the use of BSA standard curves in the determination of protein within w

113 A reproducible standard curve is generated with a EC(50) of approximate

114 tissue homogenates (10 ng/g tissue), and the standard curve is linear up to 1000 ng/mL, with precisio

115 lute concentration of target sequence when a standard curve is not available.

116 receptors, but also the medium in which the standard curve is run.

117 ernal standards DA-d4 and DOPAC-d5 result in standard curve linearity for DA from 0.05-100 ng/mL (LOD

118 two methods of calculating fold increase: a standard curve method and the DeltaDelta Ct method.

119 estimates from C(q) calibration fitting (the standard curve method).

120 ose a novel framework that combines existing standard curve methods into a multidimensional standard

121 gene copy numbers were achieved by relative standard curve methods using cloned C4 genomic DNA cover

122 Further, we conducted a series of standard curve migration assays for basal media suppleme

123 us in all samples was quantitated by using a standard curve obtained by serial dilution of an in vitr

124 After comparison with standard curves obtained by serial dilution of DNA extra

125 Standard curves obtained from 2-ml aliquots of BAL fluid

126 oreover, in an experiment directly comparing standard curves obtained from band densitometry and thre

127 Islet sizes were extrapolated from standard curves obtained using microspheres from which i

128 A standard curve of band densities was generated by using

129 ody levels were determined by reference to a standard curve of fluorescent intensity generated using

130 Based upon a TaqMan-generated standard curve of infectious WN virus, the limit of dete

131 A standard curve of Leu-enkephalin was performed in the pr

132 3.0 values of <50 copies/ml by extending the standard curve of the assay showed that these samples wi

133 des, we establish a monotonic yet non-linear standard curve of the DeltaD/ - Deltaf ratio as a functi

134 By comparison with a known standard curve of tm versus log[Na+] for poly(dA) . poly

135 Standard curves of antibiotic concentration versus ECL i

136 ion, integrated ion counts were derived from standard curves of authentic compounds of phosphatidylch

137 Standard curves of neat authentic standards and spiked e

138 new method was developed for preparation of standard curves of spiked tissue homogenates, based on t

139 Standard curves of the spiked calibrants were generated

140 g SPSS V 16.0 (P < 0.05) and quantified with standard curve on GCDC acid.

141 oplets at high resolution to encode multiple standard curves on the surface of a single 1 mm(2) SERS

142 Use of a potato starch standard curve over-estimated starch concentrations.

143 ] vs 69 [56-87]% of maximum concentration in standard curve; p=0.043; n=8 and n=6, respectively).

144 The average accuracy for the standard curve points in extracted human plasma was 99-1

145 A standard curve prepared for methylphenidate in urine (R(

146 enrichments were calculated by comparison to standard curves prepared from dA and [(2)H(2)]-dA.

147 t values of adK, tcdA and tcdB obtained from standard curves presented an excellent linear fit (slope

148 Internal bovine serum albumin standard curves provided a method to assess on-chip PF2D

149 olates were quantified using three validated standard curve qPCR assays targeting adK, tcdA and tcdB

150 otal RNA concentrations and agrees well with standard curve qPCR.

151 quantification by SQT-DNA is as reliable as standard curve quantification for a wide range of DNA co

152 m 15 to 30% at all concentrations within the standard curve range.

153 area ratios at all concentrations within the standard curve ranges were compared.

154 data using three different methods (absolute standard curve, relative standard curve, and comparative

155 CLIA with a standard curve resulted in 36.4% (80/220) positivity.

156 Using two different materials to generate a standard curve revealed that the Octaplex TaqMan assay a

157 The software estimates standard curves, sample protein concentrations and their

158 The resulting standard curves showed good linearity and high sensitivi

159 For these assays, standard curves showing correlation between target conce

160 f infectious virus/reaction) and efficiency (standard curve slope = -3.66).

161 Precision values of standard curves slopes were lower than 3.4% and recovery

162 shed to be 31.25pM with the linearity of the standard curve spanned to 2500pM.

163 The qualified range of the standard curve spans 6 orders of magnitude from 2.5 x 10

164 LysA cDNA detection varied linearly across a standard curve that matched the sensitivity of quantitat

165 iter (CFU/mL) were estimated by generating a standard curve that plotted quantitative polymerase chai

166 calculated from the regression equation of a standard curve that was generated by plotting the logari

167 a new methodology based on multidimensional standard curves that extends the use of real-time PCR da

168 nalyses of these assays use methods based on standard curves that have limitations in detecting low o

169 eiver operating characteristic (ROC) curves, standard curves that represent item recognition across d

170 rived relationship to generate a theoretical standard curve, the measured ratio of the M (monoisotopi

171 Based on a linear standard curve, the species' absorbance at 1104 cm(-1) i

172 Notably, this approach does not rely on standard curves to determine isobaric impurity compositi

173 Based on these data, a standard curve up to 2.5muM Cyt c was established.

174 inescent assay was conducted by developing a standard curve using known concentrations of PSA.

175 Standard curves using four different bacterial species g

176 ti-PS2 immunoblot signals by comparison with standard curves using radiolabeled PS1 and PS2 standards

177 A method was developed for creating standard curves using surrogate tissue sections from bla

178 Standard curves utilized both extractions from RSV cultu

179 There were negligible readings for standard curves utilizing copper in place of iron.

180 A standard curve was constructed showing a large range of

181 these fluorescence intensities, an in vitro standard curve was created based on the in vivo exposure

182 The standard curve was established from QCM-D responses and

183 A standard curve was found to be linear in the range of 1.

184 A standard curve was generated and developed with TaqMan(R

185 leimide-activated bovine serum albumin and a standard curve was generated for each blot.

186 adenine was 0.02 microM (2.4 ng/ml), and the standard curve was linear up to 27.3 microM.

187 A linear standard curve was obtained between 10(1) and 10(8) DNA

188 omplex, multidimensional abundance corrected standard curves was thereby avoided.

189 The linearity of standard curves was up to 5000 pM.

190 ss than 100 CFU/ml and theoretically using a standard curve, was 2 CFU/ml.

191 l of the normalized endpoint intensity (NEI) standard curve, we estimate the viral load from the seru

192 Final standard curves were calculated for each pathogen by plo

193 Standard curves were constructed (R(2)>0.99) to allow fo

194 Standard curves were created for alginate in deionised H

195 After standard curves were developed for quantification of C1-

196 Standard curves were established by relating the MGIT ti

197 The standard curves were fitted to a quadratic regression ov

198 Full standard curves were generated for 23 different assays.

199 CFU estimations by all three standard curves were highly reproducible, regardless of

200 In fact, the raw data for the standard curves were highly scattered and non-linear, re

201 Standard curves were linear from 25 fmol to 2 pmol for p

202 Opiate standard curves were linear from the LOQ to 12500 pg/mg.

203 Standard curves were linear from the LOQ to 5000 pg/mg f

204 Standard curves were linear over a range of 0.0 to >4.5

205 Standard curves were linear over a range of 5-1000 ng fo

206 The standard curves were linear over the range from 2 ng/mL

207 400 ng/mL), good linearities (r2 > 0.99) for standard curves were obtained.

208 And internal standard curves were used to clarify the changes in thei

209 pace, data points do not fall exactly on the standard curve, which enables a similarity measure betwe

210 LightCycler Q-PCR conditions, we obtained a standard curve with a linear range (correlation coeffici

211 ed from the observed ion intensities using a standard curve with curve parameters unaffected by the p

212 lysates were then determined by generating a standard curve with defined amounts of a highly purified

213 In addition, a standard curve with up to five points is generated, resu

214 above the biosensor's top layer, generating standard curves with R(2) > 0.99.

215 d as a surrogate matrix for human to prepare standard curves without endogenous interference.