ライフサイエンスコーパス: sticky end

1 GT/-3', producing four base 3'-OH overhangs (sticky ends).

2 DNA template adjacent to the gene, leaving a sticky end.

3 junctions linked together with complementary sticky ends.

4 ze on a nucleosome, generating two vacant CD sticky ends.

5 ell-structured branched DNA motifs tailed by sticky ends.

6 ity, was also constructed by the addition of sticky ends.

7 uppressed in the presence of gaps around the sticky ends.

8 ami constructs, hybridizing or dehybridizing sticky ends.

9 the intermolecular contacts are directed by 'sticky' ends.

10 sembly of cohesive single-stranded segments (sticky ends).5, 6 Methods that exploit the sequence addr

11 ective Watson-Crick base-pairing between the sticky ends and bivalent antibody-antigen binding, such

12 and of DNA composed entirely of concatenated sticky ends and that binds to four local neighbours duri

13 re is also a logical equivalence between DNA sticky ends and Wang tile edges.

14 tent state in which two unbound chromodomain sticky ends appear exposed.

15 Such a 'sticky end' approach subverts existing atom insertion-de

16 ends are flanked by a pair of dyes; when the sticky ends are disrupted, the dyes separate, and the fl

17 Those sticky ends are flanked by a pair of dyes; when the stic

18 depend on the rotational positioning of the sticky ends around the helical axis is less clear.

19 -assembly processes, with complementary DNA "sticky ends" as one of the most notable examples.

20 ate configurations, for example, homodimers, sticky-ended assemblies, and antiparallel arrangements.

21 aspartate to stabilize the triple helix in a sticky-ended assembly.

22 Complementary sticky ends associate with each other preferentially and

23 ation of the three different tiles and three sticky end association strategies.

24 bility and cooperativity of a network of DNA sticky-end associations could lead to greater control ov

25 son-Crick base-pairs and a two-nucleotide 5'-sticky end at each end of the duplex.

26 Specifically, sticky ends at crystal contacts were enzymatically ligat

27 nucleosomal distance, suggesting that the CD sticky ends bridge nearby methylated nucleosomes.

28 on an edge adjacent to the binding site; the sticky ends can be disrupted if the protein binds with s

29 ed CMP pairs helps to explain the success of sticky-ended CMP association and changes the conception

30 The sticky ends cohere with one another, so the molecules fo

31 constructs controllably switch ON or OFF the sticky end cohesion and in turn cascade to the structura

32 cilitated by immobile Holliday junctions and sticky end cohesion.

33 Sticky-end cohesion plays a critical role in molecular b

34 al assembly critically relies on intermotif, sticky-end cohesion, which requires complementarity not

35 ound that 5'-phosphorylation strengthens the sticky-end cohesion: in a DNA crystal self-assembled exc

36 ination of synthetic stable branched DNA and sticky-ended cohesion has led to the development of stru

37 hnology combines branched DNA junctions with sticky-ended cohesion to create self-assembling macromol

38 uous arrays facilitated by sequence-specific sticky-ended cohesion.

39 produce well-ordered 2D periodic arrays via sticky-ended cohesion.

40 embled into objects and networks directed by sticky-ended cohesion.

41 a DNA crystal self-assembled exclusively via sticky-end cohesions, 5'-phosphorylation not only promot

42 gate the modulation of 5'-phosphorylation on sticky-end cohesions.

43 e combination of branched DNA molecules and 'sticky' ends creates a powerful molecular assembly kit f

44 used as symmetry-breaking synthons (when the sticky ends deliberately consist of orthogonal sequences

45 work, we demonstrate for the first time that sticky ended dimers are not a prerequisite for alpha-hel

46 f such nanofibers has been the formation of "sticky ended" dimers through careful selection of electr

47 s and is analogous to the "reprogramming" of sticky-ends displayed on the DNA tiles.

48 g the drive force compared to single pair of sticky ends DNA machine (the six-helix bundle DNA origam

49 Sticky-ended DNA duplexes can associate spontaneously in

50 at the modified base, and the other across a sticky-ended DNA junction.

51 Herein, we describe "sticky-ended" DNA triplex-quadruplex composites that spe

52 irections of propagation associated with the sticky ends do not share the same plane, but extend to f

53 n site allows to precisely organize multiple sticky-end-encoded oligo segments into double-stranded (

54 in charged pairs, and is inspired by similar sticky-ended fibrillation designs applied in DNA and coi

55 Complementary 'sticky ends' form specific inter-particle links and repr

56 ion electron microscopy indicate that these "sticky-ended" fragments self-assemble via intermolecular

57 re that geometric hindrance does not prevent sticky ends from associating.

58 e TX-TL system using specifically engineered sticky-end genes that are gated through T7-Locks on a tr

59 lking between Watson-Crick and non-canonical sticky ends in which the ratio between the two dictates

60 the interior of the frame through prescribed sticky end interactions.

61 y triangles that forms through non-canonical sticky end interactions.

62 cifically designed arm lengths and intertile sticky-end interactions can be used to form sophisticate

63 Cyclization of DNA with sticky ends is commonly used to measure DNA bendability

64 ation with complementary single-stranded DNA sticky ends is increasingly used for guiding the self-as

65 The strength of the sticky ends is readily varied, so that the ability of th

66 e multiple cloning site via either blunt- or sticky-end ligation not only serves as a highly efficien

67 on-Crick complementary single-stranded DNA ("sticky end") linking strategies.

68 Employing a "sticky end"-mediated molecular strategy for constructing

69 inside aquafoldamers can be created via the "sticky" end-mediated formation of 1D chiral helical stac

70 Free energies of sticky-end mismatches are also calculated for determinin

71 We show that the T4 DNA ligase repairs sticky ends more efficiently than blunt ends and that th

72 ogrammed temperature change in milliseconds, sticky ends of a compound DNA origami machine can open a

73 ive adsorption properties of the two 12-base sticky ends of the DNA molecules to partially immobilize

74 rmation of the 12 base-pair single-stranded "sticky" ends of mature lambda DNA.

75 f the duplex, thus forming the 12-base-pair "sticky" ends of the mature genome.

76 The two DX molecules are also joined by sticky ends on an edge adjacent to the binding site; the

77 ber, position, chirality, and flexibility of sticky ends on each square, we assemble uniformly sized

78 ary the number of linkers, the length of the sticky ends on the linker, and linker architecture and m

79 ntrolled by the number and orientation of EE sticky ends on the PAEs.

80 r of single-stranded oligonucleotides (i.e., sticky ends) on its surface.

81 oping) rates (kloop and kunloop) of DNA with sticky ends over three helical periods (100-130 bp) usin

82 ray assembly including T-loop configuration, sticky end pairing, structural diversification, and vari

83 shared-stem design for the LNA-MB to prevent sticky-end pairing.

84 Functionalized with DNA with single-stranded sticky ends, patches on different particles can form hig

85 these crystals is dependent on the number of sticky ends per EE, while lattice symmetry is controlled

86 nd the generation of unique, non-palindromic sticky ends permits the formation of seamless junctions

87 the number and the relative position of DNA sticky-ends play a significant role in the stability of

88 r backbone, self-complementary "palindromic" sticky ends readily form intraparticle hairpins and loop

89 DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target lead

90 Efficient NHEJ of sticky ends requires the Ku70 and Ku80 proteins and the

91 Assembly through sticky ends requires the recognition of a single strand

92 o program a hexagonal arrangement using: the sticky end sequence; triangle edge torsional stress; and

93 improvement (2.6 angstrom) using a modified sticky-end sequence.

94 ally modified with DNA bearing complementary sticky end sequences.

95 For DNA motifs with exactly the same pair of sticky-end sequences, by adjusting the length (thus, hel

96 modifications (1) confirms the importance of sticky-end stacking, (2) confirms the identity of the in

97 e the cross linkers are elastic springs with sticky ends stochastically binding to and unbinding from

98 the solution an oligonucleotide duplex with 'sticky ends' that are complementary to the two grafted s

99 tural units are programmed by the design of 'sticky ends' that associate according to Watson-Crick co

100 For our 11 base "sticky ends," the limit is 73 distinct sequences with no

101 When the DNA dendrimers have identical sticky ends, they hybridize with DNA-functionalized nano

102 Sticky ends thus created are uniform across the assembly

103 quare origami with 10 pairs of complementary sticky ends to drive a bead on the end of a rod like ori

104 chieved using reactants with non-palindromic sticky ends to maximize specificity.

105 ranched DNA molecules are linked together by sticky ends to produce objects, periodic arrays, and nan

106 , we find that the backbone that tethers the sticky ends to the surface can have a significant impact

107 These had "sticky-ends" to promote the formation of long fibers.

108 f collagen-mimetic peptides (CMPs) that form sticky-ended triple helices has allowed the production o

109 g three spacer-18 units and a 4-6 nucleotide sticky end was used, structures with greater crystallini

110 methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal pr

111 l are the free energies of ligation for each sticky end, which can be estimated by the calculator fro

112 hree arms terminate with single-stranded DNA sticky ends while the fourth arm is end-conjugated with