ライフサイエンスコーパス: stopped-flow

1 solved fluorescence decay every 0.1 ms after stopped flow.

2 tentiometric transition time with respect to stopped flow.

3 ase of production rates of batch-mode (i.e., stopped-flow) (129)Xe hyperpolarizers, which is particul

4 -20 mol %), has been investigated by in situ/stopped-flow (19)F NMR spectroscopic analysis of the kin

5 Iron oxidation experiments using stopped-flow absorbance spectroscopy revealed an extreme

6 2-NrdF, NrdI(hq), and O2 has been studied by stopped flow absorption and rapid freeze quench EPR spec

7 n the absence of polysaccharide substrate by stopped-flow absorption and rapid freeze-quench (RFQ) el

8 vity of the Cu(I) site was probed by EPR and stopped-flow absorption spectroscopies, and a rapid one-

9 ir reactivity with O2 and NO was analyzed by stopped-flow absorption spectroscopy and amperometric me

10 With stopped-flow absorption spectroscopy, we detected and co

11 Also, anaerobic stopped-flow analyses revealed that the equilibrium diss

12 QR with the use of steady state kinetics and stopped flow analysis.

13 this complex process has been achieved using stopped-flow analysis but, due to limitations in instrum

14 Stopped-flow analysis demonstrated ordered assembly of H

15 Stopped-flow analysis of GTP-binding and GDP/GTP exchang

16 calorimetry, surface plasmon resonance, and stopped-flow analysis, we demonstrate that Ca(2+) binds

17 By rapid kinetic stopped-flow analysis, we identified the molecular mecha

18 Using stopped flow and other spectroscopic techniques, the the

19 oxylases was investigated in single turnover stopped flow and quench flow measurements of biotin tran

20 Stopped-flow and electrochemical measurements have been

21 mechanism in greater detail, spectrochemical stopped-flow and electrochemical techniques were employe

22 oscopy, using conventional spectrometers and stopped-flow and laser-flash techniques.

23 ction was investigated using single turnover stopped-flow and quench-flow assays.

24 Stopped-flow and rapid-quench flow experiments of HadA w

25 poration forward and reverse reactions using stopped-flow and rapid-quench methods with a T7 DNA poly

26 We used stopped-flow and steady-state kinetics experiments, alon

27 onance Raman spectroscopy, pulse radiolysis, stopped flow, and electrospray ionization mass spectrome

28 ciation of these proteins using rapid-mixing stopped flow, and examine how the kinetic behavior is pe

29 Detailed electrochemical, stopped-flow, and NMR studies of the Co(III)-OH to Co(IV

30 chemical trapping of reaction intermediates, stopped-flow, and substrate hydrogen isotope exchange te

31 A home-built stopped-flow apparatus is interfaced to a Hadamard trans

32 as to optical data obtained with the present stopped-flow apparatus.

33 asuring the peak currents (Ip) produced in a stopped flow approach.

34 med by nanosecond laser-flash-photolysis and stopped-flow are accompanied by resonance Raman and FT-I

35 DNA polymerase activity was measured by a stopped-flow assay that monitors polymerase extension us

36 The fluorescence stopped-flow assay, therefore, provides a high-throughpu

37 robust sequential-mixing fluorescence-based stopped-flow assay.

38 he results of biochemical, rapid-quench, and stopped-flow assays revealed that differences in biochem

39 d activation in purified SthK channels using stopped-flow assays, mutagenesis, enzymatic catalysis an

40 Polyphasic stopped-flow association and dissociation kinetics suppo

41 Equilibrium and stopped-flow binding assays and size exclusion chromatog

42 e-chase, equilibrium-binding titrations, and stopped-flow binding studies.

43 X and other CA isoforms was determined via a stopped-flow, CA-catalyzed CO2 hydration assay.

44 ep required 1 min at 1.6 V vs. Ag/AgCl under stopped flow conditions, which was preceded by a 10 s ac

45 Stopped flow data and luminescence resonance energy tran

46 Stopped-flow data show that, upon the initiation of the

47 Our stopped-flow data showed that the 420-nm intermediate wa

48 Fits of UV-visible stopped-flow data suggested a three-step model and provi

49 Pre-steady state kinetic data obtained in a stopped-flow device show that the initial binding of JBP

50 ever, traditional enzyme mixing methods like stopped-flow do not allow for the observation of fast pr

51 Rapid stopped-flow estimation of the transient-state constants

52 More interestingly, the stopped-flow estimations of electron transfer rates also

53 Consistent with this analysis, stopped flow experiments find betaine mostly affects DHF

54 Using quench flow and stopped flow experiments in a biochemical system for pro

55 reactivity are investigated by double-mixing stopped-flow experiments for both complexes.

56 its complex with DNA were analyzed by using stopped-flow experiments in which fluorescence changes i

57 Stopped-flow experiments indicate a pK(a) = 10.4 for DMS

58 Stopped-flow experiments revealed a approximately 2.7-fo

59 Stopped-flow experiments showed that pyrrole-based inhib

60 Anisotropy stopped-flow experiments showed that the kinetics descri

61 Stopped-flow experiments then demonstrated that the chan

62 We used quantum chemical computations and stopped-flow experiments to evaluate a chemical mechanis

63 Stopped-flow experiments using the dye RH421 show that F

64 Single turnover stopped-flow experiments were used to identify catalytic

65 Furthermore, concentration-dependent stopped-flow experiments with both factors reveal positi

66 Pre-steady-state, stopped-flow experiments with cefoxitin revealed a burst

67 en determined by a combination of mixing and stopped-flow experiments with spectrophotometric monitor

68 oliposome water permeability with the aid of stopped-flow experiments yielded a unitary water permeab

69 electron paramagnetic resonance, and optical stopped-flow experiments, along with calculations using

70 le multiparameter fluorescence spectroscopy, stopped-flow experiments, and molecular dynamics simulat

71 In stopped-flow experiments, we observed that cYY binding t

72 Using stopped-flow experiments, we show that RFS-1/RIP-1 confe

73 ing partial reaction kinetics determined via stopped-flow experiments, we studied cholesterol's effec

74 mumol.m(-2).s(-1) for various conditions in stopped-flow experiments.

75 spectroscopic (optical, EPR), and kinetics (stopped-flow) experiments on variants in which residue T

76 and K48-linked IQF-diubiquitin by USP2 using stopped-flow florescence under a single-turnover conditi

77 We conducted stopped-flow fluorescence analyses of the binding of mon

78 -association using dynamic light scattering, stopped-flow fluorescence and circular dichroism spectro

79 Contradicting this assumption, we present stopped-flow fluorescence and NMR experiments that give

80 RNase footprinting, in vitro binding and stopped-flow fluorescence annealing assays showed that a

81 ystem was measured by circular dichroism and stopped-flow fluorescence as a function of pH and concen

82 berculosis rrnAP3 ribosomal promoter using a stopped-flow fluorescence assay.

83 Using stopped-flow fluorescence data for the target associatio

84 Although stopped-flow fluorescence data suggest that melittin tet

85 Stopped-flow fluorescence experiments revealed that kine

86 Stopped-flow fluorescence experiments showed that anneal

87 rmotoga maritima CheA was investigated using stopped-flow fluorescence experiments that monitored bin

88 Stopped-flow fluorescence measurements with Mycobacteriu

89 In this study, using a stopped-flow fluorescence method, we examined the kineti

90 Here, using the stopped-flow fluorescence method, we measured the target

91 NA complex, using laser temperature jump and stopped-flow fluorescence methods with U1A proteins labe

92 Stopped-flow fluorescence resonance energy transfer expe

93 Lastly, stopped-flow fluorescence spectrophotometry showed that

94 Fourier-transform infrared and high-pressure stopped-flow fluorescence spectroscopies were applied.

95 We used stopped-flow fluorescence spectroscopy and single-molecu

96 Using stopped-flow fluorescence spectroscopy to measure associ

97 e KIS native-state equilibrium obtained from stopped-flow fluorescence studies of refolding His-tagge

98 of temperatures and pressures by means of a stopped-flow fluorescence technique.

99 Stopped-flow fluorescence techniques were used to determ

100 ciated with interconversion and unfolding by stopped-flow fluorescence to determine transition-state

101 sing steady-state and stopped-flow kinetics, stopped-flow fluorescence, and rapid-freeze-quench EPR.

102 Stopped flow fluorimetry illuminated the conformational

103 Stopped-flow fluorimetry experiments demonstrated that t

104 DNA was detected by EMSA, steady-state, and stopped-flow fluorimetry.

105 m thermoautotrophicum homologue (MthK) and a stopped-flow fluorometric assay for fast channel activat

106 we use isothermal titration calorimetry and stopped-flow fluorometry to determine and analyze the th

107 Here, we used stopped-flow Forster resonance energy transfer to invest

108 Here, we applied stopped-flow Fourier transform infrared and electron-nuc

109 on of electron paramagnetic resonance (EPR), stopped flow freeze quench on a millisecond-second time

110 Stopped-flow FRET and fluorescence anisotropy show that

111 Transient kinetic analysis and stopped-flow FRET demonstrated that the R712G mutation s

112 Stopped-flow FRET studies indicated that such frustrated

113 For the first time, our stopped-flow FRET studies revealed that a DNA polymerase

114 ng translocation in real time using ensemble stopped-flow FRET with ribosomes containing fluorescent

115 physics approach, incorporating rapid-mixing stopped-flow, high-pressure, and CD spectroscopies.

116 ometer was integrated into a custom-designed stopped-flow injection device to collect visible absorpt

117 The system utilizes a conventional stopped-flow injection system coupled to a modified low

118 In this study, we use a rapid mixing stopped-flow instrument to study the kinetics of CO(2) i

119 presented through kinetics measurements (by stopped-flow IR spectroscopy), X-ray crystal structure a

120 By means of NMR, stopped-flow IR, and quenched-flow techniques, the kinet

121 Stopped flow kinetic analysis was used to confirm this p

122 AD(*-) or alpha-FADH(-), respectively, using stopped flow kinetic measurements.

123 Stopped flow kinetic studies of the OAT reactions show a

124 tingly, isothermal titration calorimetry and stopped-flow kinetic analyses reveal uncoupled nucleotid

125 nt (17)O NMR spectroscopy, electrochemistry, stopped-flow kinetic analyses, and EPR measurements were

126 Stopped-flow kinetic analysis of the DypB-catalyzed reac

127 Furthermore, stopped-flow kinetic analysis of Zika NS5-, RdRp- and MT

128 Stopped-flow kinetic and spectroscopic titration studies

129 Stopped-flow kinetic data demonstrate flavin oxidation w

130 Stopped-flow kinetic data were obtained for the iron-typ

131 tween PFASs and hCAs were investigated using stopped-flow kinetic enzyme-inhibition measurements, nat

132 Rapid-scanning stopped-flow kinetic experiments demonstrated that subst

133 As shown through stopped-flow kinetic experiments, electron transfer capa

134 Using a combination of steady-state and stopped-flow kinetic experiments, substrate analogs, and

135 nformational changes through equilibrium and stopped-flow kinetic measurements of correct nucleotide

136 Stopped-flow kinetic measurements of sugar binding with

137 Stopped-flow kinetic measurements show differences in co

138 Rapid stopped-flow kinetic measurements show that increasing h

139 Forster resonance energy transfer (FRET) and stopped-flow kinetic measurements, we monitored Ca(2+)-i

140 near diffusion-limited on rates, as shown by stopped-flow kinetic measurements.

141 nding to ssDNA by equilibrium titrations and stopped-flow kinetic measurements.

142 k(CPET) values with those from prior thermal stopped-flow kinetic studies gives data sets for the oxi

143 Stopped-flow kinetic studies indicate that increased fle

144 Stopped-flow kinetic studies involving alterations in th

145 Here we report stopped-flow kinetic studies of azide binding to human f

146 Flavin fluorescence and stopped-flow kinetic studies on CaM-bound enzymes sugges

147 Stopped-flow kinetic studies on UTP binding followed by

148 Stopped-flow kinetic studies show that the oxidative tra

149 = 3,5-Me(2)C(6)H(3)) with O(2) was shown by stopped-flow kinetic studies to result in the rapid form

150 In stopped-flow kinetic studies with alpha,beta-methylenead

151 Stopped-flow kinetic studies, examining the impact of tr

152 y detectable C4a-(peroxy)flavin formation in stopped-flow kinetic studies.

153 second-order rate constants, measured using stopped-flow kinetic techniques, spanned 4 orders of mag

154 Transient stopped-flow kinetic traces at various final TMAO concen

155 city and high affinity for iNOS, using rapid stopped-flow kinetic, gel filtration, and spectrophotome

156 Stopped flow kinetics were used to measure the equilibri

157 side-chain groups in bulk measurements using stopped-flow kinetics and NMR spectroscopy.

158 Single-molecule microscopy and stopped-flow kinetics assays were carried out to underst

159 leotide conditions and carrying out parallel stopped-flow kinetics assays.

160 Stopped-flow kinetics at variable acidities in H(2)O and

161 Single-turnover stopped-flow kinetics experiments demonstrate that incre

162 We report stopped-flow kinetics experiments to study the folding a

163 Stopped-flow kinetics found that Cu(A) formation proceed

164 Here we dissect the E*-E equilibrium with stopped-flow kinetics in the presence of excess ligand o

165 Stopped-flow kinetics of sugar binding by WT LacY in det

166 Stopped-flow kinetics revealed that the reduction of VAO

167 Stopped-flow kinetics showed slower unfolding at around

168 Stopped-flow kinetics studies monitoring the change in t

169 l analysis of these mutations based on rapid stopped-flow kinetics to determine elongation rates and

170 re, we develop a radiolabeling assay and use stopped-flow kinetics to establish that FXN is functiona

171 To resolve this controversy, we used stopped-flow kinetics to provide evidence for a catalyti

172 e combined molecular dynamics simulation and stopped-flow kinetics with fluorescence detection to tra

173 haracterized by single-turnover kinetics and stopped-flow kinetics with fluorescent detection.

174 Crystallography, stopped-flow kinetics, quench-flow reactions, and infect

175 e reaction mechanism, using steady-state and stopped-flow kinetics, stopped-flow fluorescence, and ra

176 Here, using site-directed mutagenesis and stopped-flow kinetics, we examined the reactive form of

177 alpha-helices to be targeted selectively by stopped-flow kinetics, X-ray crystallography, and soluti

178 ycloserine was performed by pre-steady-state stopped-flow kinetics.

179 In this latter case, stopped-flow light scattering analysis reveals the trans

180 Osmotic water permeability was measured by stopped-flow light scattering in human and rat erythrocy

181 Stopped-flow light scattering measurements confirmed tha

182 enal cortical membrane vesicles, measured by stopped-flow light scattering, was reduced in CAII-defic

183 rated water channel activity, as revealed by stopped-flow light scattering.

184 Kinetics obtained from stopped-flow measurements are corroborated by current pl

185 mic parameters obtained from low-temperature stopped-flow measurements are in excellent agreement wit

186 Additionally, stopped-flow measurements have shown that the rate of ve

187 Experimental data obtained from stopped-flow measurements of the refolding of Escherichi

188 FRET-based stopped-flow measurements revealed that Atg18 rapidly ol

189 Stopped-flow measurements show that the formation of the

190 Targeted amino acid mutagenesis and stopped-flow measurements substantiate the functional re

191 Stopped-flow measurements suggest that initial PROPPIN-m

192 In this work, we have performed fluorescence stopped-flow measurements to investigate the kinetics of

193 The data from native MS and stopped-flow measurements were in excellent agreement.

194 High-pressure stopped-flow measurements were performed at pH 8, which

195 Fluorescence stopped-flow measurements were used to determine rate co

196 ough isothermal titration calorimetry (ITC), stopped-flow measurements, mutagenesis studies, and mole

197 than it bound large unilamellar vesicles in stopped-flow measurements.

198 separation properties were characterized by stopped-flow measurements.

199 n-rate is too fast to detect by conventional stopped-flow measurements.

200 eir ECD spectra have been recorded online by stopped-flow measurements.

201 t with steady state data confirming that the stopped-flow method used was appropriate for the reactio

202 with nitrones were determined using a UV-vis stopped-flow method, and phenyl radical (Ph(*)) trapping

203 have developed single-turnover fluorescence stopped-flow methods that allow us to quantitatively exa

204 In this work, we used stopped-flow methods to monitor the coupling of adenosin

205 uorescence anisotropy and total fluorescence stopped-flow methods to the analysis of polypeptide tran

206 Fluorescence stopped-flow methods were used to record the kinetics of

207 Using single-turnover fluorescence stopped-flow methods, here we report that ClpA, when ass

208 electrochemistry (PFE), and pre-steady-state stopped-flow methods.

209 further, we undertook kinetic studies using stopped-flow methods.

210 cation of spectrophotometric monitoring with stopped-flow mixing has been used to explore the role of

211 ormed transient time-resolved FRET following stopped-flow mixing of actin with labeled myosin, preinc

212 We employed a stopped-flow mixing technique to systematically investig

213 ay scattering (SAXS) in combination with the stopped-flow mixing technique, the entire micelle format

214 ECD spectra were measured in the stopped-flow mode and compared with results from quantum

215 torr Xe in 500 cc) in either single-batch or stopped-flow mode, negating in part the usual requiremen

216 (25 mM NaOH), and 250 V for only 10 min in a stopped-flow mode, the extraction recoveries for the mu-

217 hyperpolarized propane gas were revealed by stopped-flow MRI visualization at 4.7 T.

218 Stopped-flow NMR measurements suitable for determination

219 the conjugation of absorption spectroscopy, stopped-flow, NMR, and X-ray crystallography.

220 s where the dimeric species predominates, by stopped flow, observing prominent electrostatic sensitiv

221 rmediate Ni layer (for magnetic guidance and stopped flow operations) and PtNPs inner catalytic layer

222 = 9%) anti-CRP functionalization, controlled stopped-flow operations as well as efficient bubble prop

223 equent characterizations by a combination of stopped-flow optical absorption spectroscopy and freeze-

224 ts electronic and geometric structure, using stopped-flow optical and advanced paramagnetic resonance

225 luorescent nucleotides without using a rapid stopped-flow or quench-flow instrument and the generally

226 In this report, stopped-flow polarization was utilized to determine the

227 (Thal) from Streptomyces albogriseolus using stopped-flow, rapid-quench flow, quantum/mechanics molec

228 nd thermal-induced unfolding experiments and stopped-flow refolding studies at ambient pressure, NMR-

229 An electroporation apparatus hyphenated with stopped-flow sample injection permits the introduction o

230 n into DrDps2 was investigated by static and stopped-flow SAXS measurements, revealing dynamic struct

231 Using stopped-flow spectrofluorimetry, association rate consta

232 x-ray scattering, circular dichroism, and a stopped-flow spectrofluorometric assay, respectively.

233 Stopped flow spectrofluorometry analysis of recombinant

234 found to be >10(6) M(-1) s(-1) at pH 7 using stopped-flow spectrometry.

235 on reaction occurs in the mixing time of the stopped-flow spectrophotometer when arginine is the subs

236 ages during their reaction with DPPH using a stopped-flow spectrophotometer-based method.

237 troscopy of the oxidative half-reaction in a stopped-flow spectrophotometer.

238 lamine (DEA) has been investigated using the stopped-flow spectrophotometric technique at 25.0 degree

239 d alkanolamines have been investigated using stopped-flow spectrophotometry and (1)H NMR measurements

240 Based on results from stopped-flow spectrophotometry, the reduced enzyme-3HB c

241 molecule was kinetically characterized using stopped-flow spectrophotometry.

242 ption at 25 degrees C was investigated using stopped-flow spectrophotometry.

243 Stopped-flow spectroscopic data revealed that transfer o

244 Here, we use stopped flow spectroscopy to show that the CTD of Escher

245 To address this, we used stopped-flow spectroscopy and computer modeling approach

246 Using stopped-flow spectroscopy and laser-triggered NO release

247 x1)-dependent peroxidase assays conducted by stopped-flow spectroscopy demonstrated that V(max,app) i

248 ange of saturated fatty acids (C12-C20), and stopped-flow spectroscopy indicates a rapid reaction bet

249 Stopped-flow spectroscopy results indicated rapid initia

250 Pre-steady-state analyses using stopped-flow spectroscopy revealed a KIE of 3 that can b

251 data and THF dissociation rates measured by stopped-flow spectroscopy shows that product release can

252 In this study, we employed difference stopped-flow spectroscopy to characterize reaction inter

253 Stopped-flow spectroscopy was employed to elucidate the

254 Using stopped-flow spectroscopy, we determined rate constants

255 By employing stopped-flow spectroscopy, we have carried out a compara

256 Using double-mixing stopped-flow spectroscopy, we investigated the reactions

257 ociation and dissociation were determined by stopped-flow spectroscopy, yielding a k(f) and k(b) of (

258 d in the presence of SBDS using fluorescence stopped-flow spectroscopy.

259 rate the nickel(II) complex was monitored by stopped-flow spectroscopy.

260 ic parameters obtained from ITC, UV/vis, and stopped-flow spectroscopy.

261 The stopped-flow studies revealed a complete reaction pathwa

262 Stopped-flow studies using these two distinct tools reve

263 Here, we present the first multimixing stopped-flow study on a fully functional truncated varia

264 nd I and compound II state in a multi-mixing stopped-flow study.

265 a rapid binding reaction can be followed by stopped-flow synchrotron-radiation circular dichroism (C

266 inetic-spectrophotometric data acquired by a stopped-flow system for the quantitation of tartrazine i

267 An additional modification leads to a stopped-flow system very efficient for instance for 2D N

268 Using the stopped-flow technique, we found that the binding reacti

269 anions, as evaluated by pulse radiolysis and stopped flow techniques.

270 Using stopped-flow techniques and FRET-melting/annealing assay

271 benzoic acid (p-NBA) were investigated using stopped-flow techniques at 4 degrees C.

272 ear magnetic resonance, crystallography, and stopped-flow techniques to an enzyme designed for an ele

273 We used spectroscopic and stopped-flow techniques to further investigate how the c

274 with certain substrates has been observed by stopped-flow techniques when [(Ph3P)CuH]6 is treated wit

275 Using stopped-flow techniques, we systematically compare the r

276 d using static binding, chemical quench, and stopped-flow techniques.

277 ess the binding to MCL-1 using time-resolved stopped-flow techniques.

278 plex formation by fluorescence titration and stopped-flow techniques.

279 ansient kinetics approaches (quench-flow and stopped-flow) to determine how subunit-specific mutation

280 )N4(6-Me-DPEN))(O2)](+) (4), is observed (by stopped-flow) to rapidly and irreversibly form in this r

281 as sampled by Fpg, as evident from the F110W stopped-flow traces, but less extensively than oxoG.

282 bed the mechanism of heme capture using NMR, stopped-flow transfer kinetics measurements, and molecul

283 This enabled a stopped-flow type of experiment to measure the (initiall

284 Stopped-flow UV-vis absorption coupled with rapid-freeze

285 Employing stopped-flow UV-vis methods, reactions were triggered by

286 ectivity for CO(2) over H(+) was observed by stopped-flow UV-vis spectroscopy of [Re(bipy-tBu)(CO)(3)

287 hosphine (PAr3) substrates were monitored by stopped-flow UV-vis spectroscopy, and revealed second-or

288 In contrast, the kinetics (via stopped-flow UV-vis) for complex 3-O displayed a signifi

289 ed-valence CuA and its M160SeM derivative by stopped-flow UV-vis, EPR, and XAS at both Cu and Se edge

290 Herein we report stopped-flow UV-visible (UV-vis) spectroscopy, X-ray cry

291 ls and detected with the use of rapid-mixing stopped-flow UV-visible (UV-vis) spectroscopy.

292 Stopped-flow UV-visible absorption and freeze-quench Mos

293 ivity of recombinant human CBS by static and stopped-flow UV-visible absorption spectroscopy.

294 e role(s) that tyrosyl radicals play in DHP, stopped-flow UV-visible and rapid-freeze-quench EPR spec

295 The OER is also examined with stopped-flow UV-visible spectroscopy, and its kinetic be

296 oxyferrous states using biochemical assays, stopped-flow UV-visible, and rapid-freeze-quench electro

297 Experimental measurements from stopped-flow UV/vis spectrophotometry afforded derivatio

298 roteo mass spectrometry and conventional and stopped-flow UV/visible spectroscopy, was used in conjun

299 ence on either side of LacY were measured by stopped flow with LacY in detergent or in proteoliposome

300 rates of substrate binding were measured by stopped-flow with purified LacY either in detergent or i