ライフサイエンスコーパス: strand exchange

1 the ATP hydrolysis to work (in this case DNA strand exchange).

2 eins are responsible for homology search and strand exchange.

3 specificity in a manner that stimulates DNA strand exchange.

4 2-MND1 as a 'molecular trigger' of RAD51 DNA strand exchange.

5 ure able to promote homology recognition and strand exchange.

6 in the B core site is required for the first strand exchange.

7 represents an intermediate in the process of strand exchange.

8 hat it is required upstream of RecA-mediated strand exchange.

9 egrees about the flat exchange interface for strand exchange.

10 tion of homologous joint molecules (JMs) and strand exchange.

11 on and signalling or promote ATP-independent strand exchange.

12 ional DNA repair through homology search and strand exchange.

13 1-Tid1 tilt the bias toward interhomolog DNA strand exchange.

14 leavages of the donor template necessary for strand exchange.

15 search for homologous DNA sequences and DNA strand exchange.

16 ons for the mechanism and preferentiality of strand exchange.

17 ncrease the directionality and stringency of strand exchange.

18 is an ATPase that mediates recombination via strand exchange.

19 ved recombinase, catalyses homology-directed strand exchange.

20 ration dependence parallels that seen in DNA strand exchange.

21 nd breaks (DSBs) to create the substrate for strand exchange.

22 ning reaction between DNA partners, yielding strand exchange.

23 s Rad51 homologue, SsoRadA, to stimulate DNA strand exchange.

24 ngle-stranded tails necessary for subsequent strand exchange.

25 DNA (ssDNA), but not dsDNA, to stimulate DNA strand exchange.

26 current models linking ATP hydrolysis to DNA strand exchange.

27 is is completely uncoupled from extended DNA strand exchange.

28 but is insufficient for accommodating donor strand exchange.

29 role in facilitating DinB's activity during strand exchange.

30 l separation of DNA pairing and extended DNA strand exchange.

31 major role in, and may be required for, DNA strand exchange.

32 As are paired and available for extended DNA strand exchange.

33 d polymer on single-stranded DNA to catalyze strand exchange.

34 ences that are located distal to the site of strand exchange.

35 th both Rad52 and Rad51 and stimulated Rad51 strand exchange.

36 ight coupling between ATP hydrolysis and DNA strand exchange.

37 ent on DNA double-strand break formation and strand exchange.

38 binds RAD51, the enzyme responsible for DNA strand exchange.

39 f UvsX-ssDNA filaments that is active in DNA strand exchange.

40 gle-stranded DNA for recombinase loading and strand exchange.

41 y the archaeal RecA homolog RadA to catalyze strand exchange.

42 ments mediate the homologous DNA pairing and strand exchange.

43 A strands in D-loops formed by RecA-mediated strand exchange.

44 t DNA double-strand break repair via inverse strand exchange.

45 results in stimulation of RAD51-promoted DNA strand exchange.

46 filament, in the direction of RecA-mediated strand exchange.

47 aments to a conformation more proficient for strand exchange.

48 promote joint molecule formation to initiate strand-exchange.

49 the overlap regions between the sites of the strand exchanges.

50 ic complex and orchestrates the order of DNA strand exchanges.

51 In strand exchange a single-stranded DNA (ssDNA) bound to R

52 Rad51 mediates DNA strand exchange, a key reaction in DNA recombination.

53 In vitro, HOP2-MND1 stimulates the DNA strand exchange activities of RAD51 and DMC1.

54 of Srs2, providing a means for tailoring DNA strand exchange activities to enhance the fidelity of re

55 in is a novel inhibitor of RecA-mediated DNA strand exchange activities.

56 In vitro, RAD52 has ssDNA annealing and DNA strand exchange activities.

57 02, indicating that it blocks the ATPase and strand-exchange activities of PfRad51 and abrogates the

58 Unexpectedly, we find that RAD51's strand exchange activity is not required to convert stal

59 1 (B02), which specifically inhibits the DNA strand exchange activity of human RAD51.

60 rprisingly, we found that BLM stimulates DNA strand exchange activity of RAD51.

61 The UvsX-H195Q mutant retains weak DNA strand exchange activity that is inhibited by wild-type

62 -stranded DNA (ssDNA) and showed more robust strand exchange activity with oligonucleotide substrates

63 Surprisingly, it possesses the strand exchange activity without RAD51.

64 ilament remodeling, fail to stimulate RAD-51 strand exchange activity, demonstrating that remodeling

65 with reduced ssDNA-binding affinity and DNA strand-exchange activity in both H195Q and H195A mutants

66 A recombination resulting from RecA-mediated strand exchange aided by RecBCD proteins often enables a

67 ment has long been known to occur during DNA strand exchange, although its importance to this process

68 h this, we show that FANCJ can inhibit RAD51 strand exchange, an activity that is likely to be import

69 actions between hRad54 and hRad51 during DNA strand exchange and branch migration, which are two core

70 Moreover, N-DBD stimulates the inverse strand exchange and can use DNA and RNA substrates.

71 characteristic dsDNA extension rates due to strand exchange and free RecA binding are the same, sugg

72 RAD54 promotes RAD51-mediated DNA strand exchange and has been described to both stabilize

73 s identify temporal coordination between DSB strand exchange and homolog pairing as a critical determ

74 chanistic explanation for DinB's function in strand exchange and improves our understanding of recomb

75 Rad51, for DNA binding, filament stability, strand exchange and interaction with the antirecombinase

76 recombination by making an initial cleavage, strand exchange and ligation, followed by strand swappin

77 lap region, followed by the second cleavage, strand exchange and ligation.

78 by a sequence identity-independent cleavage, strand exchange and ligation.

79 n vitro, RAD54 stimulates RAD51-mediated DNA strand exchange and promotes branch migration of Hollida

80 xpression of Rad51, a protein central to DNA strand exchange and recombination, did not further incre

81 ly depends on both RecA-catalyzed homologous strand exchange and RuvABC-catalyzed Holliday junction r

82 mechanism normally blocks multiple rounds of strand exchange and triggers product release after a sin

83 J isomerization then allows a second pair of strand exchanges and thus formation of the final recombi

84 WINKLE: strand-separation, strand-annealing, strand-exchange and branch migration suggest a dual role

85 discuss how RdgC might inhibit RecA-mediated strand exchange, and how RdgC might be displaced by othe

86 NA nucleoprotein filaments that catalyze DNA strand exchange, and it mediates single-strand DNA annea

87 the RecA protein, including DNA binding, DNA strand exchange, and LexA protein cleavage.

88 airing, which includes DNA/RNA annealing and strand exchange, and mediator, which is to assist RAD51

89 in concert with SsbA and DprA, catalyzes DNA strand exchange, and SsbB is an accessory factor in the

90 ecA filament assembly and the subsequent DNA strand exchange are directional.

91 chanisms of RAD-51-DNA filament assembly and strand exchange are well characterized, the subsequent s

92 be recognized by PcG complexes, and RNA-DNA strand exchange as a PRC2 activity that could contribute

93 tly published structural data that implicate strand exchange as part of a mechanism for IKK2 activati

94 sites that flank the region of cleavage and strand exchange, as well as six arm-type sites.

95 Strand-exchange assays were performed specifically to as

96 sfer to study the mechanism of Dmc1-mediated strand exchange between DNA oligonucleotides with differ

97 cA homologs Rad51 and Dmc1 are essential for strand exchange between homologous chromosomes during me

98 It polymerizes onto DNA and promotes strand exchange between homologous chromosomes.

99 d51 in eukarya and RadA in archaea) catalyse strand exchange between homologous DNA molecules, the ce

100 tein of HR, possesses a unique activity: DNA strand exchange between homologous DNA sequences.

101 We show that in eukaryotes, inverse strand exchange between homologous dsDNA and RNA is a di

102 tion with wild type Flp, Flp(R191A) promotes strand exchange between MeP- and P-DNA partners.

103 Although providing an efficient rate of DNA strand exchange between polymorphic alleles, Dmc1 must a

104 h BRCA2 protein stimulates DMC1-mediated DNA strand exchange between RPA-ssDNA complexes and duplex D

105 emia helicase FANCM, prevents precocious DSB strand exchange between sister chromatids before homolog

106 The rate of strand exchange between the oligonucleotides increased 8

107 All three variants are proficient in DNA strand exchange, but G151D is slightly more sensitive to

108 for DNA repair, including RAD51 mediated DNA strand exchange, but is dispensable for DNA replication.

109 The resolvase Sin regulates DNA strand exchange by assembling an elaborate interwound sy

110 dies established that Brh2 can stimulate DNA strand exchange by enabling Rad51 nucleoprotein filament

111 Only the first group enhances DNA strand exchange by RAD51.

112 networks, leading to rapid and efficient DNA strand exchange by Rad51.

113 se was intentionally slowed, consistent with strand exchange by random walk in which rate declines pr

114 t for sequence identity within the region of strand exchange, called the overlap region.

115 herein we demonstrate that synthetic helical strand exchange can be achieved through tuning of poly(m

116 processive "360 degrees rotation" rounds of strand exchange can be observed, if the recombining site

117 Our results show how RNA strand exchange can expand the utility of RNAi computing

118 a much more central role in DNA pairing and strand exchange catalyzed by DrRecA than is the case for

119 but not SsbB, DprA was able to activate DNA strand exchange dependent on RecA . ATP.

120 w RAD51 promotes replication restart by both strand exchange-dependent and strand exchange-independen

121 the peptide and its use as a tool to dissect strand exchange-dependent DNA repair within cells.

122 haliana revealed that it forms an unexpected strand-exchange dimer in which the ATP-binding P-loop an

123 The defining step of HR is homologous strand exchange directed by the protein RAD51, which is

124 The products of extended DNA strand exchange do not form.

125 Namely, HOP2-mediated strand exchange does not require ATP and, in contrast to

126 ereas most mismatches near the 5' end impede strand exchange dramatically.

127 Nte) of an incoming pilus subunit by a donor-strand exchange (DSE) mechanism.

128 ered to the outer membrane usher where donor strand exchange (DSE) replaces PapD's donated beta stran

129 esion DNA polymerase active in RecA-mediated strand exchange during error-prone double-strand break r

130 ion of RAD51 from heteroduplex DNA following strand exchange during homologous recombination.

131 Dmc1 catalyzes homology search and strand exchange during meiotic recombination in budding

132 We propose that strand exchange during recombination events within guani

133 on of DNA partners and the directionality of strand exchange during recombination mediated by tyrosin

134 Here, we illuminate how strands exchange during meiotic recombination in male mi

135 Topo I could play an active role in strand exchange, either by altering the kinetics or ther

136 drolysis is not required for DNA pairing and strand exchange, eliminating active search processes.

137 contrast, RecA . dATP efficiently catalyzes strand exchange even in the absence of single-stranded b

138 locus, and provide insights into aspects of strand exchange events between paralogous sequences in t

139 ts from non-crossovers to crossover-specific strand exchange, explaining Mph1's apparent anti-crossov

140 o different dominant-negative alleles of the strand exchange factor, Rad51.

141 e-stranded DNA was generated; the homologous strand-exchange factor, Rad51, accumulated into foci; a

142 sequences called loxP after which a pair of strand exchanges forms a Holliday junction (HJ) intermed

143 unbinding of non-homologous dsDNA and drives strand exchange forward for homologous dsDNA.

144 This differential extension drives strand exchange forward for homologs and increases the f

145 less extended incoming strand, which drives strand exchange forward.

146 f HIV-1 nucleocapsid protein, which promotes strand exchange, had little effect on this outcome.

147 irement for RecA filament disassembly in DNA strand exchange has a variety of ramifications for the c

148 s align homologous sequences and promote DNA strand exchange has long been known, as are the crystal

149 After strand exchange, heteroduplex dsDNA is bound to site I.

150 s interactions with the RecA filament during strand exchange, identifying key contacts made with resi

151 C-terminal autoregulatory flap, also promote strand exchange in a 5'-to-3' polarity in ATPgammaS, a p

152 ive, small interfering RNA molecules via RNA strand exchange in a cell-free Drosophila embryo lysate,

153 ng and point toward the possibility of using strand exchange in a native biological setting.

154 ormed DNA heteroduplex and transient reverse strand exchange in a weaving type of mechanism.

155 critical component of HR and facilitates DNA strand exchange in DSB repair.

156 al Rad51 paralogs that cooperate to catalyse strand exchange in eukaryotes.

157 These proteins normally promote DNA-DNA strand exchange in homologous recombination.

158 he hypothesis that DMC1, not RAD51, performs strand exchange in mammalian meiosis.

159 It enables RAD51 DNA strand exchange in the absence of divalent metal ions re

160 red particle motion, we show that successful strand exchange in the presence of ATP proceeds with a 5

161 DprA facilitates RecA-mediated DNA strand exchange in the presence of both SSB proteins.

162 randed DNA binding protein ICP8 and promotes strand exchange in vitro in conjunction with ICP8.

163 In any eukaryote, RAD51-directed strand exchange in vivo is mediated by further factors,

164 d activity of yeast and human Rad52: inverse strand exchange, in which Rad52 forms a complex with dsD

165 estart by both strand exchange-dependent and strand exchange-independent mechanisms.

166 that Mph1 and Mus81-Mms4 recognize an early strand exchange intermediate and direct repair to noncro

167 and eukaryotic Rad51 and Dmc1 all stabilize strand exchange intermediates in precise three-nucleotid

168 model in which mismatch recognition reverses strand-exchange intermediates prior to the initiation of

169 erged sequences by recognizing mismatches in strand-exchange intermediates.

170 iments, we demonstrate that the mechanism of strand-exchange involves active coupling of unwinding an

171 nts improves at pH 8.5, whereas complete DNA strand exchange is also restored.

172 DNA strand exchange is also slowed commensurate with the rat

173 nition of homologous sequences of DNA before strand exchange is considered to be the most puzzling st

174 ere, we demonstrate that the polarity of DNA strand exchange is embedded within RecA filaments even i

175 The efficiency of strand exchange is highly sensitive to the location, typ

176 tal homology may be reduced if RecA-mediated strand exchange is immediately followed by DNA synthesis

177 How DNA mismatches affect Dmc1-mediated DNA strand exchange is not understood.

178 n filament disassembly and completion of DNA strand exchange is observed.

179 ments form, search for homology and catalyse strand exchange is poorly understood.

180 Homologous pairing and strand exchange lead to the formation of DNA intermediat

181 cally to inhibit RecA during an on-going DNA strand exchange, likely through the disassembly of RecA

182 Thus, the changes in tension inherent to strand exchange may couple with ATP hydrolysis to increa

183 ns that are thought to be brought about by a strand exchange mechanism involving the N-terminal extra

184 the barrel, consistent with a proposed beta-strand exchange mechanism.

185 The enzyme system's strand-exchange mechanism proceeds via a Holliday-juncti

186 peats that polymerize into a pilus through a strand-exchange mechanism.

187 common and idiosyncratic features in the DNA strand exchange mechanisms of three RecA-family recombin

188 ggest they also directly promote the DNA-RNA strand exchange necessary for hybrid formation since we

189 Strand exchange nucleic acid circuitry can be used to tr

190 These sites flank the overlap regions where strand exchanges occur.

191 interactions with one another through donor strand exchange, occurring at the usher, in which the N-

192 ction in binding free energy, and subsequent strand exchange occurs in precise 3-nt steps, reflecting

193 dinally organized chromosome axes and stable strand exchange of crossover-designated DSBs.

194 gs and increases the free energy penalty for strand exchange of non-homologs.

195 nity, it has minimal effects on WRN-mediated strand exchange of telomeric DNA.

196 d1 protein complex stimulates Dmc1-catalyzed strand exchange on homologous DNA or containing a single

197 A junctions, cleaving 1nt 3' to the point of strand exchange on two strands symmetrically disposed ab

198 ATP hydrolysis is coupled to polar strand exchange over longer distances, and its contribut

199 DNA strand exchange plays a central role in genetic recombin

200 ng yeast provide evidence that Rad52 inverse strand exchange plays an important role in RNA-templated

201 We propose that there is an inherent strand exchange polarity mediated by the structure of th

202 omponents from parting ways and initiate the strand exchange process.

203 lity in the base pairing in the heteroduplex strand exchange product could provide stringent recognit

204 efficient and stringent formation of stable strand exchange products and which is consistent with a

205 s to be incorporated in otherwise homologous strand exchange products even though sequences with less

206 f ATP hydrolysis even 75 bp sequence-matched strand exchange products remain quite reversible.

207 crease the reversibility of sequence matched strand exchange products with lengths up to approximatel

208 insight into how ATP hydrolysis destabilizes strand exchange products.

209 nd its cognate RecA led to inhibition of DNA strand exchange promoted by RecA.

210 plants, with sequence similarity to the RecA strand exchange protein and a role in homologous recombi

211 RAD51 DNA strand exchange protein catalyzes the central step in ho

212 In selecting ssDNA over dsDNA, the RAD51 DNA strand exchange protein has to overcome the entropy asso

213 ependent of RAD51, which encodes the central strand exchange protein in yeast required for conservati

214 In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation

215 The DNA strand exchange protein RAD51 facilitates the central st

216 led DNA, which serves as a substrate for the strand exchange protein Rad51.

217 iated stimulation of the other budding yeast strand exchange protein Rad51.

218 hat invokes fork regression catalyzed by the strand exchange protein RecA as an intermediate in the p

219 mulates the activity of the meiosis-specific strand exchange protein ScDmc1 only 3-fold, whereas anal

220 equires nucleases that resect DSB ends and a strand exchange protein that facilitates homology search

221 The meiosis-specific DNA strand exchange protein, DMC1, promotes the formation of

222 A strand exchange protein, RAD51, is also required for rep

223 Here we report that the S. cerevisiae DNA strand exchange protein, Rad51, prevents Rad52-mediated

224 rhang, which becomes a substrate for the DNA strand exchange protein, Rad51.

225 ease cuts the 3'-ended strand and loads RecA strand-exchange protein onto it.

226 ia coli the RecBCD enzyme also loads the DNA strand-exchange protein RecA onto the newly formed end,

227 and examined the activity of SsoSSB with the strand-exchange protein S. solfataricus RadA (SsoRadA).

228 gp59) and a Rad51/RecA analogue (the T4 UvsX strand-exchange protein).

229 AD51 and other members of the RecA family of strand exchange proteins assemble on ssDNA to form presy

230 ssed ends are substrates for assembly of DNA strand exchange proteins that mediate DNA strand invasio

231 is of homologous chromosomes, persistence of strand exchange proteins, and alterations in both the fr

232 RAD51 and DMC1 are the strand-exchange proteins forming a nucleofilament for st

233 defining member of a ubiquitous class of DNA strand-exchange proteins that are essential for homologo

234 After DNA strand exchange Rad51 protein is stuck on the double-str

235 ends of the complementary strand reduces the strand-exchange rate for homologous filaments.

236 ant protein is capable of catalyzing the DNA strand exchange reaction and is insensitive to inhibitio

237 cleoprotein filaments on DNA that catalyze a strand exchange reaction as part of homologous genetic r

238 s, how exactly hydrolysis influences the DNA strand exchange reaction at the structural level remains

239 nd Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.

240 al-time fluorescence with a toehold-mediated strand exchange reaction termed one-step strand displace

241 RecA's ability to promote LexA cleavage and strand exchange reaction, and are believed to modulate i

242 DNA annealing function to actively catalyze strand-exchange reaction between the unwinding substrate

243 The strand-exchange reaction is central to homologous recomb

244 Unlike strand-annealing, the strand-exchange reaction requires nucleotide hydrolysis

245 ge helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a s

246 capable of hybridizing to target RNA through strand-exchange reaction.

247 Strand exchange reactions catalyzed by phosphorylated ve

248 and diagnostic applications, similar to how strand exchange reactions in solution have been used for

249 ismatched base pairs that impede uncatalyzed strand exchange reactions led to a significant decrease

250 uman RAD51 protein catalyzes DNA pairing and strand exchange reactions that are central to homologous

251 bined biochemical reconstitutions of the DNA strand exchange reactions with total internal reflection

252 shape changing films that are powered by DNA strand exchange reactions with two different domains tha

253 to dif and carry out two pairs of sequential strand exchange reactions.

254 ification circuits based on toehold-mediated strand exchange reactions.

255 UvsX and RecA catalyze similar strand-exchange reactions, but differ in other propertie

256 erization on DNA substrates and catalysis of strand-exchange reactions.

257 rically activated to catalyze ATPase and DNA strand-exchange reactions.

258 RAD51 is the central strand exchange recombinase in somatic homologous recomb

259 (NPF) that catalyzes homologous pairing and strand exchange (recombinase) between DNAs that ultimate

260 ons with arm-type sites dictate the order of strand exchange regardless of the orientation of the ove

261 tes (FRTs) harboring non-homology within the strand exchange region does not yield stable recombinant

262 The identification of the most common strand exchange regions of these 78 deletions served to

263 ctions is that the first step, XerD-mediated strand exchange, relies on interaction with the very C-t

264 and DNA (ssDNA) binding, ssDNA annealing and strand-exchange (SE) activities.

265 cation (LAMP), programmable toehold-mediated strand-exchange signal transduction, and standard pregna

266 N and telomeric DNA, stimulates WRN-mediated strand exchange specifically between telomeric substrate

267 is to initiate the homology recognition and strand-exchange steps and those of hRad54 are to promote

268 aluate the utility and robustness of helical strand exchange, stereoregular PMMA/polyethylene glycol

269 ent to generate PiDSD, an intermolecular DNA strand-exchange strategy to measure a set of key kinetic

270 TPgammaS also exhibit a 5'-to-3' progress of strand exchange, suggesting that the polarity is not det

271 less effective at stabilizing RecA-mediated strand exchange than native DinB.

272 anism: a sequence identity-dependent initial strand exchange that requires two base pairs of compleme

273 egy termed ERASE (Epitaxial Removal Aided by Strand Exchange) that allows a single flow cell to be us

274 is a bystander during the annealing step of strand exchange, the enzyme strongly discriminates again

275 g strand in a D-loop formed by RecA-mediated strand exchange, the extension afforded by 82 bp of homo

276 ent sequence specificity of toehold-mediated strand exchange, the OSD reporter could successfully dis

277 gh Cre and Int use the same mechanism of DNA strand exchange, their respective reaction pathways are

278 e that hRad54 can facilitate hRad51-promoted strand exchange through various degrees of mismatching.

279 tential mismatches and facilitate long-range strand exchanges through branch migration of Holliday ju

280 other 10FNIII domain via a Trp-mediated beta-strand exchange to stabilize a partially unfolded interm

281 ures exploits Watson-Crick hybridization and strand exchange to stitch linear duplexes into finite as

282 Strand exchange usually terminates after a single round

283 d the possibility that propagation solely by strand exchange was a significant contributor to transfe

284 of bacterial genomes; however, commitment to strand exchange was believed to occur after testing appr

285 Significantly, PMMA strand exchange was demonstrated and utilized to reversi

286 the ability of RAD54 to stimulate RAD51 DNA strand exchange was not significantly affected by SN, in

287 Unlike Rad51, Brh2 was able to promote DNA strand exchange when preincubated with double-stranded D

288 ific recombinase Tn3 resolvase initiates DNA strand exchange when two res recombination sites and six

289 e complementary strand significantly retards strand exchange, whereas applying the same force to the

290 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward

291 h core-type sites adjacent to the regions of strand exchange, while the N-terminal arm binding (N) do

292 tes long 3' single-strand tails that undergo strand exchange with a homologous chromosome to form joi

293 consequence, RecA-RFP is proficient for DNA strand exchange with dATP or at lower pH.

294 in nucleotide exchange, RPA displacement and strand exchange with full-length DNA substrates.

295 single-stranded DNA (ssDNA), which catalyzes strand exchange with homologous duplex DNA.

296 ad52 forms a complex with dsDNA and promotes strand exchange with homologous ssRNA or ssDNA.

297 This concept of strand exchange with PMMA-based triple-helix stereocompl

298 ssing entails 5' end resection and preferred strand exchange with the homolog rather than the sister

299 emodeling the Rad51 filament, priming it for strand exchange with the template duplex.

300 unbinds, whereas homologous dsDNA undergoes strand exchange yielding heteroduplex dsDNA in site I an