ライフサイエンスコーパス: strand invasion

1 erties, stimulates SSA activity and promotes strand invasion.

2 p2/Mnd1) that greatly enhances Dmc1-mediated strand invasion.

3 from two homologs anneal as a consequence of strand invasion.

4 ase triplet steps during the early stages of strand invasion.

5 buted in nature: single-strand annealing and strand invasion.

6 tion (HR), where it mediates RAD51-dependent strand invasion.

7 ciate with homologous donor sequences during strand invasion.

8 ctions, suggesting that Mnd1p is involved in strand invasion.

9 can make PNAs even more powerful agents for strand invasion.

10 an ideal substrate for RecA protein-promoted strand invasion.

11 hesize as little as 30 nucleotides following strand invasion.

12 relatively short hybrid regions, suggesting strand invasion.

13 clear periphery before Rad51 recruitment and strand invasion.

14 and annealing, often with multiple rounds of strand invasion.

15 that is competent for end resection but not strand invasion.

16 for a homologous donor sequence and promotes strand invasion.

17 rified HOP2 alone is proficient in promoting strand invasion.

18 ination, suggesting DNA end resection before strand invasion.

19 rified HOP2 alone is proficient in promoting strand invasion.

20 1/2 inhibitor-induced DNA lesions before DNA strand invasion.

21 nucleoprotein filaments that can mediate DNA strand invasion.

22 ed nucleosomes in the HML donor locus during strand invasion.

23 ns of recombination, homology search and DNA strand invasion.

24 DNA lesion bypass that is distinct from DNA strand invasion.

25 ilize long duplex donors that participate by strand invasion.

26 sults in a block to meiotic DSB repair after strand invasion.

27 responsible for key mechanistic steps during strand invasion.

28 replication restart by a mechanism involving strand invasion.

29 ppresses ssDNA annealing and facilitates DNA strand invasion.

30 ingle-strand DNA substrate for RecA-mediated strand invasion.

31 OH for all markers downstream of the site of strand invasion.

32 NA strand exchange proteins that mediate DNA strand invasion.

33 ble-stranded heteroduplex DNA product of DNA strand invasion.

34 mbination performing homology search and DNA strand invasion.

35 tein bound to the heteroduplex DNA after DNA strand invasion.

36 dges Rad51 from ssDNA preventing promiscuous strand invasions.

37 Red system can operate--strand annealing or strand invasion--accounts well for these findings.

38 point mutation in Hop2 that dissociates its strand invasion activity from its ability to bind and an

39 While Rdh54/Tid1 enhances the Rad51 DNA strand invasion activity in vitro, it is unclear how it

40 This strand invasion activity of ICP8 coupled with DNA synthe

41 ssDNA may explain its ability to enhance the strand invasion activity of the T4 recombinase (UvsX) an

42 tes to the DNA lesion bypass through its DNA strand invasion activity.

43 We provide the first report of strand-invasion activity in plant mitochondria.

44 ble-strand break (DSB) triggers HR repair by strand invasion also in BRCA2-defective cells, but less

45 e cells are not completely impaired in HR by strand invasion although the spontaneous HR rate is 10-f

46 nition by the U2 snRNP as a toehold-mediated strand invasion and advance an unanticipated drug target

47 t mobile D-loops that mimic structures in HR strand invasion and at telomere ends.

48 ater step in homologous recombination, after strand invasion and before the initiation of new DNA syn

49 s process, PARP2 promotes DNA end resection, strand invasion and BIR-dependent mitotic DNA synthesis

50 T) facilitates telomere lengthening by a DNA strand invasion and copying mechanism.

51 ologous recombination protein, necessary for strand invasion and crossing over.

52 way was considerably less active than single-strand invasion and did not contribute significantly to

53 nerate condensates, and we take advantage of strand invasion and displacement reactions to kineticall

54 single-stranded DNA (ssDNA), preventing DNA strand invasion and exchange by homologous recombination

55 asion intermediates in a manner that favours strand invasion and exchange.

56 Mre11 bound to partly processed DSBs impairs strand invasion and HR.

57 Partner choice is determined at the time of strand invasion and is mediated by two recombinases: Rad

58 delays Rad51p binding to ssDNA and prevents strand invasion and localization of Rad51p to HML alpha.

59 dmc1Delta mutants are strongly defective in strand invasion and meiotic progression and that these d

60 filament engagement with the donor DNA, the strand invasion and pairing with the complementary seque

61 show that BIR synthesis initiates soon after strand invasion and proceeds more slowly than S-phase re

62 que recombination pathway dependent on Rad51 strand invasion and Rad52-Rad59 strand annealing activit

63 nduced in the palindrome promotes homologous strand invasion and repair synthesis, similar to mitotic

64 Error-free, conservative HR involves strand invasion and requires a homologous DNA template,

65 Mechanistically, we demonstrate that strand invasion and subsequent HR defects upon BRCA2 los

66 range homology search that occurs before the strand invasion and transfer reactions.

67 Dimeric bis-PNAs capable of both strand invasion and triplex formation can form clamp str

68 combinants (by single-strand annealing or by strand invasion) and to convert recombinational intermed

69 The repair did not involve Rad51-driven strand invasion, and moreover the suppression of strand

70 downstream HR steps of homology search, DNA strand invasion, and repair synthesis remain to be deter

71 Rates of strand invasion are temperature dependent and can be enh

72 nt background and was consistent with single-strand invasion/assimilation without mismatch correction

73 s a ssDNA-dependent ATPase that can catalyze strand invasion at both saturating and subsaturating con

74 sothermal nucleic acid amplification method, Strand Invasion-Based Amplification (SIBA), which exhibi

75 ctions that help ensure Dmc1-mediated stable strand invasion between homologous chromosomes, thereby

76 solution of complex entanglements (involving strand invasion between nonsister telomeres) requires ra

77 eveals that rad52-R70A cells can mediate DNA strand invasion but are unable to complete the recombina

78 Rad51, a recombinase, catalyzes strand invasion but binds ssDNA with lower affinity than

79 Pol delta complex mutants are proficient for strand invasion but unable to complete extensive tracts

80 BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is

81 t of the designed peptide increases rates of strand invasion by 100-fold relative to unmodified bisPN

82 e, 3' tails must presumably be generated for strand invasion by 5'-3' exonuclease activity.

83 c recombination mediator that stimulates DNA strand invasion by both Dmc1 and Rad51.

84 t not the individual proteins, can stimulate strand invasion by Dmc1.

85 To increase the versatility of strand invasion by PNAs, we have synthesized bisPNAs and

86 ditions should further expand the utility of strand invasion by PNAs.

87 into a higher-ordered state less amenable to strand invasion by RAD51-coated ssDNA filaments.

88 Strand invasion by RAD51-ssDNA complexes depends on ATP

89 ein onto the DSB ends at MAT, the subsequent strand invasion by the Rad51 nucleoprotein filament into

90 We propose a model in which lower-stem strand invasion by Uc.283+A impairs microprocessor recog

91 ds inside bacteria clearly demonstrates that strand invasion can take place in a biologically relevan

92 sequence targeted by the PNA is the shortest strand invasion complex reported to date.

93 Irradiation of the strand invasion complex results in asymmetric cleavage o

94 uch that the C-terminal T5sequence forms the strand invasion complex, leaving the N-terminal T5sequen

95 ugate has been synthesized and shown to form strand invasion complexes with a duplex DNA target.

96 argets in a sequence-specific manner forming strand-invasion complexes.

97 p) is the product of homology search and DNA strand invasion, constituting a central intermediate in

98 RAD51-independent recombination by one-ended strand invasion coupled to DNA synthesis, followed by si

99 l for DSB-induced gene conversion in which a strand invasion creates a modified replication fork, inv

100 n concert with Rad51 and Dmc1 to promote the strand invasion (D-loop formation) step of homologous re

101 or gaps in nonsister homologous chromatids), strand invasion disparity, and different correction freq

102 show that BIR can occur by several rounds of strand invasion, DNA synthesis and dissociation.

103 ccumulating evidence for Rad51-catalyzed DNA strand invasion during double-strand break repair featur

104 the length of hDNA formed by the initiating strand invasion event determines susceptibility of the r

105 a homologous donor sequence and a subsequent strand invasion event on chromatin fibers.

106 Strand invasion events mature into double Holliday junct

107 ant JM structures that result from secondary strand-invasion events and often require Mus81-Mms4 for

108 defect observed for rad51 mutants is due to strand invasion failure, whereas the Pol delta complex m

109 to a model where joining is initiated by 3' strand invasion followed by pairing to short repeat sequ

110 Mismatch correction of strand invasion heteroduplex DNA is strongly polar, favo

111 change proteins forming a nucleofilament for strand invasion, however, the function of the paralogues

112 Here we show that ICP8 also catalyzes strand invasion in an ATP-independent manner.

113 meiotic recombination to promote homologous strand invasion in the budding yeast Saccharomyces cerev

114 demonstrate that oligonucleotides promoting strand invasion in the DNA double helix can significantl

115 t study shows that beta protein can initiate strand invasion in vitro, as evidenced both by the forma

116 nd invasion, and moreover the suppression of strand invasion increased repair with oligonucleotides.

117 ws for the formation of the first detectable strand invasion intermediate (i.e., single-end invasion)

118 for BIR and gene conversion suggest a common strand invasion intermediate in these two recombinationa

119 ults show that WRN clearly prefers to act on strand invasion intermediates in a manner that favours s

120 nction is critical for stabilizing analogous strand invasion intermediates that exist in two separate

121 motif leads to failure in stabilizing early strand invasion intermediates, causing increased crossov

122 suggest a failure to disassemble RAD-51 from strand invasion intermediates.

123 ly one free end and are thought to repair by strand invasion into a homologous duplex DNA followed by

124 th only one end available for repair undergo strand invasion into a homologous duplex DNA, followed b

125 this amplification: a facilitation of primer strand invasion into double-stranded DNA, and a suppress

126 ts on RPA-coated, resected DNA and catalyzes strand invasion into homologous duplex DNA.

127 nsive semiconservative DNA replication after strand invasion into homologous template DNA.

128 er chromatid is repaired via RAD51-dependent strand invasion into the regenerated sister.

129 DNA synthesis after Rad51-mediated DNA strand invasion is a crucial step during recombination.

130 The ability to catalyze strand invasion is a novel activity of ICP8 and the firs

131 ed a system using purified proteins in which strand invasion is coupled with DNA synthesis.

132 Strand invasion is most efficient when pyrimidine PNAs a

133 , Ufd4/TRIP12, and Pep5/VPS11 compromise DNA strand invasion kinetics during homology-driven repair.

134 results can be explained by a model in which strand-invasion leading to gene conversion competes more

135 he structural results, TRF2 DBD stimulated a strand invasion-like reaction, associated with telomeric

136 rmation of a t-loop which is stabilized by a strand invasion-like reaction.

137 capable of promoting recombination by a DNA strand invasion mechanism.

138 SDSA requires strand invasion mediated by DmRad51, the product of the

139 In accord with a strand-invasion mode of complex formation, the pcPNA bin

140 e partially Mus81 dependent, suggesting that strand invasion occurs and the stalled intermediate is s

141 Strand invasion occurs in a highly sequence-specific man

142 Strand invasion occurs in uls1Delta cells with wild-type

143 After that, the strand invasion occurs via Watson-Crick pairing between

144 n single-stranded DNA, Rad52 likely promotes strand invasion of a double-stranded DNA molecule by sin

145 Aside from promoting Rad51-mediated DNA strand invasion of a partner chromatid, Rad54 and Rdh54

146 Strand invasion of different templates by both DSB ends

147 romosomes and that most replication involves strand invasion of internal regions by the ends of linea

148 mation, and (2) it suppresses Rad51-mediated strand invasion of sister chromatids via a Rad54-indepen

149 by coupling unwinding of U4/U6 stem II with strand invasion of stem I.

150 TRF2 forms the T-loops by stimulating strand invasion of the 3' overhang into duplex DNA.

151 mbination at telomeres, leading to increased strand invasion of the 3' overhang within t-loop junctio

152 the cleaved recipient leading to homologous strand invasion of the donor allele, the assay was perfo

153 ay due to loss of a MEK1 stimulated bias for strand invasion of the homologous chromosome.

154 for evidence that Mek1 positively stimulates strand invasion of the homologue.

155 tly and energetically facilitates the direct strand invasion offered by collapse at positions +1 to +

156 In contrast, Rad51, which mediates strand invasion, only associates with DSBs that relocali

157 e immediate vicinity of the break was not by strand invasion or strand annealing pathways.

158 s that engage homologous sequences either by strand invasion or strand annealing.

159 can be directed to either Rad51-mediated DNA strand invasion or to Rad52-mediated DNA annealing.

160 Rapid strand invasion over a wide range of experimental condit

161 These results confirm that the single-strand invasion pathway of homologous recombination is t

162 ppear to be repaired primarily by the single-strand invasion pathway of homologous recombination.

163 Thus, either the strand invasion pathway(s) in which BRCA2 operates is st

164 22 appears to function outside of the single-strand invasion pathway, but levels of etoposide-induced

165 hat are central to both strand annealing and strand invasion pathways of recombination.

166 prepares the invading telomeric overhang for strand invasion, possibly through end processing or thro

167 rporates the well-established RecA-catalyzed strand invasion process with a novel stabilizing hybridi

168 opagated throughout the oligonucleotide by a strand invasion process.

169 The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombi

170 g subsequent Rad54p-dependent formation of a strand invasion product.

171 protein, DMC1, promotes the formation of DNA strand invasion products (joint molecules) between homol

172 Hence, strand invasion products formed by ICP8 are resistant to

173 ncrease the persistence of SsoRadA catalyzed strand invasion products.

174 in spn-A, which encodes the ortholog of the strand invasion protein Rad51.

175 ecA family of recombinases catalyzes the DNA strand invasion reaction that takes place during homolog

176 ganisms employ RecA orthologues to guide the strand invasion reactions necessary for DNA recombinatio

177 roteins that catalyze homologous pairing and strand-invasion reactions.

178 ologous DNA sequences and the subsequent DNA strand invasion required to initiate HR.

179 es our results were consistent with separate strand invasion/resolution at the two ends of the target

180 Simple strategies for enhancing strand invasion should facilitate the use of PNAs: (i) a

181 SSA competes with interhomologue strand invasion significantly more successfully when Mek

182 on structure that is thought to exist at the strand invasion site of the t-loop.

183 osal that crossover interference acts at the strand invasion stage of recombination.

184 tivity of RAD54 and the RAD51-RAD54-mediated strand invasion step during displacement loop formation.

185 up a defined in vitro system for the initial strand invasion step of double-strand break repair.

186 Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required follow

187 loops (D-loops), which represent the initial strand invasion step of HR.

188 ing event that occurs in vivo during the DNA strand invasion step of HR.

189 hat mimics an intermediate formed during the strand invasion step of many recombinational processes.

190 nce that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DN

191 hows no intrinsic polarity preference at the strand invasion step that initiates double-strand break

192 These pathways share common features in the strand invasion steps, but differ in subsequent repair s

193 ys a central role in the homology search and strand invasion steps, DSBs either are not repaired or a

194 requency, or there is a separate pathway for strand invasion still functional in BRCA2-deficient cell

195 binding would also allow TRF2 to stabilize a strand invasion structure that is thought to exist at th

196 ion DNA, and on a substrate that resembles a strand invasion structure.

197 facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs)

198 talled forks, suggesting that RAD51-mediated strand invasion supports fork restart.

199 ouble-strand gap requires multiple cycles of strand invasion, synthesis, and dissociation of the nasc

200 tion requires much less homology (30 bp) for strand invasion than does RAD51-dependent repair (approx

201 These results suggest a minor pathway of strand invasion that is dependent on RAD52 but not on RA

202 be mechanistically distinct from the initial strand invasions that establish BIR.

203 esults and on the ability of TRF2 to mediate strand invasion, that TRF2 plays an essential role in HR

204 tors that load the Rad51 filament to promote strand invasion, the defining feature of HR.

205 tly to clear Rad51 from DNA after successful strand invasion, thereby enabling the downstream events

206 s the aptamer P1 helix against central helix strand invasion, thus allowing the terminator to form.

207 takes place without double strand breaks or strand invasion, thus requiring some other mechanism for

208 this we show that in vitro Rad22 can promote strand invasion to form a D-loop that can be cleaved by

209 nctions to mediate repair via homologous DNA strand invasion to form D-loops.

210 hat recombination begins with Rad51-mediated strand invasion to form DNA substrates that are matured

211 rt a model in which DmBlm acts downstream of strand invasion to unwind a D-loop intermediate to free

212 onucleotide conjugates initiate and maintain strand invasion under more stringent conditions than do

213 on permits the Rad51 proteins to promote DNA strand invasion using either 3'- or 5'-ends with similar

214 tein may regulate the choice between the DNA strand invasion versus annealing pathways.

215 to the RecA-type recombinases that catalyze strand invasion via an active search for homology, ICP8

216 Strand invasion was efficient at neutral to basic pH, a

217 onal regulatory role for Rad51 following DNA strand invasion, where Rad51-double-stranded DNA may inh

218 through the resolution of an intratelomeric strand invasion which resembles a t-loop.

219 and stabilization of stable (legitimate) DNA strand invasions, which suggests an intrinsic mechanism

220 on single-stranded DNA (ssDNA) and catalyzes strand invasion with homologous duplex DNA.

221 and exchange proceeds via multiple rounds of strand invasion with template switching in mouse.

222 chromatid repair, as distinct from enforcing strand invasion with the homologue.

223 on either 3' or 5'-ssDNA tails and promotes strand invasion without regard for the polarity of the t