ライフサイエンスコーパス: stringency

1 der identical assay conditions (no change in stringency).

2 in or reducing vesicle size restores sorting stringency.

3 oter melting capacity for increased promoter stringency.

4 ctively, to HIF-1alpha or HIF-2alpha at high stringency.

5 ies, however, are not uniform and often lack stringency.

6 T cells rely on other mechanisms to achieve stringency.

7 g both proper timing and one round per cycle stringency.

8 ore remotely related proteins with a reduced stringency.

9 dize with the leaf form in RNA blots at high stringency.

10 cal suggestions for the control of selection stringency.

11 ates and microorganisms, even under very low stringency.

12 related deaths by 29-71%, depending on their stringency.

13 for analyses requiring very high analytical stringency.

14 I were sufficient to impart this selectivity/stringency.

15 s in both catalytic efficiency and substrate stringency.

16 tified 1,757 phosphorylation sites with high stringency.

17 uch as photonically controlled hybridization stringency.

18 requirement for MeCP2 markedly increases in stringency.

19 triphosphates incorporation with sufficient stringency.

20 applicable for the problems that demand high stringency.

21 d not adopt VCOG-RECIST guidelines varied in stringency.

22 uality-based trimming across a wide range of stringencies.

23 tion of telomerase at different purification stringencies.

24 Several high stringency 14-3-3 binding sites that overlapped with Akt

25 ng 3 rounds of phage selection of increasing stringency 6 unique scFvs that bound purified TSP by enz

26 At high stringency, 70%-90% of all annotated genes are detected

27 the investigator to decide on the filtering stringency according to desired data variability, and ou

28 re, we report the identification of 337 high-stringency allele-specific H3K4me3 and H3K36me3 peaks in

29 Using high-stringency analysis criteria (changes of twofold or grea

30 llenged with CRPV at low, moderate, and high stringencies and monitored for papilloma formation.

31 e of the salt results in increased substrate stringency and dependence on the full GroE system for re

32 The stringency and evolutionary forces governing between-hos

33 choice of mapper and variant caller, mapping stringency and filtering of SNPs by read mapping quality

34 lleviated by altering denaturation/digestion stringency and indicates why protein calibrators may not

35 The high stringency and low sample volumes of NECEEM, although ge

36 Thus, by altering the stringency and partially impairing negative selection, t

37 here ground on this work and illustrate the stringency and successful general applicability of our a

38 important but incomplete description of the stringency and thermodynamic origin of metal-ion selecti

39 massively parallel pipeline to achieve high stringency and throughput.

40 h water"-is frequently used to determine the stringency and types of measures put in place to manage

41 th of cells, their responses to stresses and stringencies, and the virulence of pathogens.

42 s and regulatory targets, tunable regulatory stringency, and rapid response to input availability.

43 including assembling contigs using variable stringencies, assembling contigs using fingerprints from

44 SAR parallels between the two series lost stringency at distal positions.

45 Reduced stringency at this checkpoint may obstruct the B cell re

46 ding an elastic system that varies selective stringency based on homeostatic demands.

47 also suggest that increasing climate policy stringency beyond a certain degree may lead to diminishi

48 ture-specific probes do not require the high stringency binding conditions necessary for methods base

49 ng phospho-RNA-seq data using a custom, high-stringency bioinformatic pipeline, we identified mRNA/ln

50 of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate

51 e UniGene gene index was screened using high stringency BLAST against a pool of endothelial expressed

52 could be amplified under various degrees of stringency by PCR or hybridized by Southern blotting fro

53 24-qPCR test on positive samples, increasing stringency by requiring a second positive result.

54 Here we report that this stringency can be altered by the nature of different pro

55 holds of phylogenetic conservation and motif stringency can be altered dynamically to facilitate deta

56 he objective of the screening procedure, its stringency can be modified by altering the level of expr

57 ove the prediction accuracy; increasing rule stringency can improve predictions and reduce extraneous

58 o a situation where a slow rise in selection stringency caused by a changing environment leads to dir

59 single-stranded DNA under both low and high stringency conditions (0.5 M NaCl).

60 t support primer extension/elongation at low stringency conditions during PCR sample preparation when

61 f proteins co-immunoprecipitating under high stringency conditions from peak Xenopus ENaC/Apx-contain

62 of Rpa1 bound single-stranded DNA under low stringency conditions only (0.1 M NaCl), while a central

63 Ikaros and Helios associated under moderate stringency conditions only with other members of the Ika

64 n blot with a p2y3 receptor probe, under low-stringency conditions that allowed the clear identificat

65 a melanogaster genomic library using reduced stringency conditions to identify closely related genes.

66 PCR (under high-stringency conditions) with primers for the mur-2(ed) ge

67 However, under low stringency conditions, both tagged proteins assembled in

68 bridised to the rye 1RS chromatin under high stringency conditions, indicating the presence of shared

69 ction digestion and hybridization under high-stringency conditions, the epa gene cluster of OG1RF (wh

70 Under reduced-stringency conditions, three additional proteins bind to

71 y Southern hybridization analyses under high-stringency conditions, we found that DNA fragments conta

72 this method include probe concentration and stringency conditions.

73 s, B. pilosicoli, or B. murdochii under high-stringency conditions.

74 ized with those isolates only under very-low-stringency conditions.

75 an additional member was detected under low-stringency conditions.

76 fragment containing the cap1 genes under low-stringency conditions.

77 bioengineered without c-MYC achieve highest stringency criteria for bona fide cardiogenesis enabling

78 Using high stringency criteria to define in vitro vertebrate topois

79 ifferences persist when we use (1) different stringency cutoffs, (2) different selection inference mo

80 single TFF (Trefoil factor) domains as high stringency determinants.

81 Low-stringency DNA blot analysis indicated that F3H is encod

82 PCR and high-stringency DNA hybridization confirmed mutagenesis and t

83 High stringency DNA hybridization confirmed the presence of t

84 to involve increased translation initiation stringency during stress-induced eIF2alpha-P, we observe

85 a nonoptimal Region 2.3 to increase promoter stringency, enabling them to mount a focused response to

86 And hybridization at low stringency ensures that all targets will be preferably h

87 ng and that nucleation site stability is the stringency factor that prevents in vivo polymerization.

88 hat different dynein functions have distinct stringencies for motor performance.

89 sis generation and imposes considerably more stringency for accuracy in identifying informative gene

90 rm cross-beta-sheet structures of sufficient stringency for incorporation into the amyloid fibril.

91 rast, IS1630 appears to exhibit an increased stringency for inverted repeat recognition, since the ma

92 istration of exogenous IFN-alpha relaxes the stringency for selection in the germinal centers resulti

93 -B27-peptide complexes might have rather low stringency for the peptide sequences.

94 Mapping stringency for type assignment was determined based on 8

95 Low-stringency genomic sequence analysis of targeted regions

96 Low stringency genomic Southern blot analysis indicates that

97 Generation of a hybridization stringency gradient allows perfect-matched hybrids to be

98 The stringency gradient is achieved by carrying out the hybr

99 ngements with similarity at a high degree of stringency (>99%) across the otherwise unrelated and div

100 n control rabbits (at the moderate challenge stringency) had a 0% probability of remaining disease fr

101 Here we report the isolation, by low stringency homology cloning from a hypothalamic library,

102 ersary, we here report a 90.4% complete high-stringency human proteome blueprint.

103 G2, a strain that is, as determined by high-stringency hybridization analysis, genetically distinct

104 High-stringency hybridization detected only one MDR-like gene

105 Low-stringency hybridization in a Southern analysis reveals

106 We used low-stringency hybridization of a chicken embryonic cDNA lib

107 (DBD) has previously depended mainly on low stringency hybridization of cDNA libraries and degenerat

108 orebrain and its microvasculature using high stringency hybridization of poly(A)+ RNA isolated from t

109 similar (sima) gene was isolated using a low-stringency hybridization screen employing a Dm single-mi

110 /EBP alpha, beta, gamma, and delta using low-stringency hybridization screening and computer searches

111 the chicken homologue was isolated using low-stringency hybridization screening of a T-lymphocyte cDN

112 nuclear genome as single-copy genes, but low-stringency hybridization suggested the presence of a mul

113 lled CaNIK1 identified in C. albicans by low stringency hybridization using CaSLN1 as a probe.

114 merase chain reaction amplification, and low stringency hybridization we have identified six novel fr

115 Through relaxed-stringency hybridization, we obtained the cDNA encoding

116 Low- as well as high-stringency hybridizations detected only a single rpoN ge

117 Increased statistical stringency identified 158 and 252 unique PVAN and TCMR i

118 High stringencies identify a core set of highly conserved gen

119 Moreover, data from low stringency immunoprecipitation experiments suggest that

120 Using a low stringency immunoprecipitation of human FcRn, we observe

121 og of FcRn is endowed with unexpectedly high stringency in binding specificity for IgGs.

122 COG technique was modified so that variable stringency in COG construction was possible.

123 iple parameter sets with different levels of stringency in computation of an optimal alignment of two

124 tumor latency, suggesting a need for greater stringency in dietary assignment for transgenic mouse mo

125 Curiously, the Q91N mutant exhibited stringency in discriminating between correct and incorre

126 ly detected significant associations at high-stringency in each population, gene-based Joint-GWAS rev

127 d during evolution and is detectable at high stringency in invertebrates.

128 brary by systematically tuning the selection stringency in screening.

129 rived peptide mass spectra require increased stringency in their interpretation.

130 nic hybridization of the target DNA, at high stringency, in <5 min.

131 sed to generate call sets of varying quality stringency, including consensus calls present across all

132 articipants' sex, years of practice, and the stringency index (within-group z score of initial 3 rati

133 ondition (good vs poor performances) and the stringency index jointly accounted for 45% of the observ

134 20% among benchmark sequences at the lowest stringency, indicating that many genes in public databas

135 High stringency is critical to avoid slippage of hybrids alon

136 Sequence stringency is imparted by specific Hoogsteen hydrogen bo

137 This overall stringency is relaxed in the 11 S regulator (PA26)-20 S

138 What remain unknown are when and with what stringency Lck is required for effective TCR-mediated ac

139 d second version of miRDeep suggest that the stringency level of each of these programs may, in fact,

140 Different stringency levels are likely to affect the number of pos

141 comparisons suggest that, despite differing stringency levels they all identify a similar set of kno

142 d a combined caller across the full range of stringency levels, which outperforms all of the individu

143 ion of reporter gene expression at different stringency levels.

144 ilies, called tribes, using three clustering stringencies (low, medium and high).

145 lysis using the Pfam server identified a low stringency match to a fatty acid desaturase domain in th

146 The increased stringency may be explained by a mild temperature sensit

147 chicken CD30 promoter has 15 predicted high-stringency Meq-binding transcription factor recognition

148 aded bioinformatic searches we devised a low stringency method for isolating proteins from an Arabido

149 High stringency Northern blotting revealed a tissue-specific

150 miRBase annotations and identifying at high stringency novel miRNAs and their targets.

151 ify the incidence of ERE sequences under two stringencies of detection: <10% and 10-20% nucleotide de

152 re: self-MHC and self-peptide diversity, the stringencies of positive and negative selection, and the

153 es on MS2 VLPs and that allow control of the stringency of affinity selection through the manipulatio

154 The stringency of ARC-seq can be scaled to accommodate the q

155 This may be useful for altering the stringency of binding enrichment during phage display.

156 It is important to elucidate the stringency of bottlenecks during transmission to shed li

157 It is possible that the lower stringency of CD8(+) T cell activation obviates a requir

158 es-specific determinant which influences the stringency of cellular response to microtubule depolymer

159 e pole morphology, chromosome alignment, and stringency of checkpoint signaling, resulting in error-p

160 uncondensed chromatin, and to determine the stringency of chromatin binding.

161 demand-side efficiency, and changes with the stringency of climate targets.

162 A measure of transmissibility and of the stringency of control measures required to stop an epide

163 quires additional regulators to increase the stringency of control, and as will be discussed, to gain

164 Since then, the stringency of criteria for HLA matching have been libera

165 differing clinical outcomes improve with the stringency of criteria, underscoring the need for compar

166 on makes a small contribution to the optimal stringency of current policy or to the overall social co

167 User defined inputs specify the stringency of data filtering and the method and conditio

168 ase-activated state and thereby decrease the stringency of decoding.

169 This has resulted in decreased stringency of diagnostic criteria, which in turn has res

170 Stringency of dietary control varied from addition of su

171 g that FOXF1 is required for maintaining the stringency of DNA replication and genomic stability.

172 he K65R substitution appears to increase the stringency of dNTP binding.

173 end on insecticide development over time and stringency of environmental regulation.

174 Adjusting the stringency of hybridization enabled single-base mismatch

175 hybridization based nucleic acid sensors the stringency of hybridization poses a challenge to design

176 Confounding factors may relate to stringency of inflammatory disease diagnosis and the eff

177 ing on the start codon, thereby altering the stringency of initiation.

178 been extensively characterized in vitro, the stringency of its search and sensitivity to mismatched s

179 yeast (Saccharomyces cerevisiae) include the stringency of metabolic control at the level of oxygen c

180 Such stringency of migration most likely imposes an immense c

181 ynonymous codon usage affects the efficiency/stringency of mRNA decoding, mRNA biogenesis/stability,

182 although BAFF/APRIL inhibition increases the stringency of negative selection of the naive autoreacti

183 This order, combined with the stringency of negative selection, is shown to lead to ef

184 We quantify the stringency of our methodology and relate it to previous

185 celerated SMG-1 kinase activity and enhanced stringency of phosphorylation site selection.

186 its output that allows the user to vary the stringency of predictions.

187 nctions of sigmas is critical to setting the stringency of promoter recognition.

188 e multiplicity to enhance the efficiency and stringency of regulation.

189 we show targets COPII protein to reduce the stringency of retention.

190 s indicate that DNA hairpins can enhance the stringency of RNase H targeted degradation of the RNA si

191 The trait allele frequencies, the stringency of sample ascertainment, the number of marker

192 ese high frequencies appear to be due to the stringency of selection with promoterless constructs, be

193 el can be inferred directly from data on the stringency of selection, and that, in agreement with rec

194 increase in error rates was affected by the stringency of selection, the trait allele frequency, and

195 se-containing media was used to increase the stringency of selection.

196 7 of HBV core antigen (HBcAg), lost the high stringency of selectivity in genome maturity during viri

197 ogical studies and a concomitant increase in stringency of significance thresholds.

198 elationship between intron abundance and the stringency of splice-site requirements; (iii) the tenden

199 The stringency of start codon selection impacts the efficien

200 e codons in preferred context to examine the stringency of start codon selection in the model filamen

201 disruption of bridges B3 and B7b alters the stringency of start codon selection.

202 ound that many P-site mutations increase the stringency of start codon selection.

203 NA(i)(Met), but have opposite effects on the stringency of start codon selection: the CTT increases,

204 Two metrics of stringency of stomatal control of psi, (1) a 'hydroscape

205 vs. anisohydry has been used to describe the stringency of stomatal regulation of plant water potenti

206 ated new and previously developed metrics of stringency of stomatal regulation of psi during soil dry

207 ydrolysis to increase the directionality and stringency of strand exchange.

208 tions instead of Mg(2+) often diminishes the stringency of substrate selection and incorporation fide

209 strain is proposed to result from a relaxed stringency of termination by RF1 at the stop codon (UAG)

210 ggests that Btk plays a critical role in the stringency of the B cell response, whereas T cells rely

211 As the DNA length and stringency of the binding reaction increase, greater pre

212 Finally, the stringency of the checkpoint response was enhanced in mu

213 ing a catalytic structure are related to the stringency of the chemistry catalyzed and define a funct

214 n by other C/EBP family proteins and the low stringency of the consensus sequence.

215 eling may vary dramatically depending on the stringency of the criteria for selecting evidence to use

216 To investigate the stringency of the Escherichia coli selenocysteine insert

217 e selectable to allow the user to decide the stringency of the homologies.

218 chromosomes a significant relaxation in the stringency of the methylated state of the paternal allel

219 Given the high stringency of the model it is remarkable that we were ab

220 lack of response to univalents: (1) reduced stringency of the oocyte SAC to aberrant chromosome beha

221 or four rounds of selection by tailoring the stringency of the panning conditions.

222 The data also highlight the stringency of the Polycomb regulatory network, and show

223 that ebs1 mutations significantly reduce the stringency of the retention-based ER quality control, al

224 r IRhsp-1 and, at the same time, reduced the stringency of the spacer length requirement, with IRper-

225 des a direct and quantitative measure of the stringency of the underlying genetic bottleneck, making

226 l outcomes were monotonically related to the stringency of the viral challenge.

227 egulated throughout life, and nowhere is the stringency of this requirement more evident than during

228 ocyte stimulator (BLyS/BAFF) may enhance the stringency of TR-->FO selection by increasing TR B cell

229 LF1 transcription may therefore modulate the stringency of viral latency.

230 thought to play a key role in regulating the stringency of viral latency.

231 Mechanistically, the motif stringency of XBP1s promoter binding sites dictates XBP1

232 prise the 28 TIGR Gene Indices, we used high-stringency pair-wise sequence searches and a reflexive,

233 Additionally, we have used a low-stringency PCR cloning technique to identify ligands, re

234 A low stringency polymerase chain reaction (PCR) homology scre

235 Using the techniques of relaxed stringency polymerase chain reaction and genomic library

236 Using a low stringency polymerase chain reaction cloning method, we

237 phylogenetic domains was combined with high-stringency protein secondary structure prediction.

238 NOD FACTOR PERCEPTION encodes a putative low-stringency receptor that is responsible for calcium spik

239 -LIKE KINASE3 (LYK3) encodes a putative high-stringency receptor that mediates bacterial infection.

240 The increased stringency required for genome-wide analyses (with per-S

241 Increased selection stringency resulted in a distinct D-peptide inhibitor te

242 elatively rigid pocket would provide greater stringency, resulting in higher fidelity of DNA synthesi

243 y with radiolabeled chicken P2Y3 cDNA at low stringency revealed a cDNA clone encoding a novel G prot

244 Varying COG stringency reveals differences in the degree of conserva

245 ds were evaluated by exposure to a series of stringency rinses in solutions of successively lower ion

246 The signature set of 29 'high stringency' RIPD genes identified here were enriched for

247 hybridization to be carried out at very low stringency (room temperature), enabling a highly selecti

248 High stringency screening of a human aortic cDNA library for

249 Low stringency screening of a human kidney cDNA library and

250 -like homeobox genes were cloned through low-stringency screening of Arabidopsis cDNA libraries with

251 a second generation of mutagenesis and high-stringency screening, we identify several significantly

252 lso identified a gene related to Mstn by low-stringency screening.

253 unctional mutants in each library using high-stringency selection (>19,000-fold), the x factor was fo

254 t Saccharomyces cerevisiae under the highest stringency selection conditions, and PKA phosphorylated

255 amase with its protein inhibitor BLIP by low-stringency selection of a random TEM1 library using yeas

256 High stringency sequence comparison (e < 1 x10(-25)) of 157 g

257 luding oligonucleotide length, hybridization stringency, sequence identity, sample abundance, and sam

258 sembled according to user-defined filter and stringency settings.

259 We used a high-stringency similarity search by using BLASTN, and a prot

260 irrhinum AG orthologue, PLENA (PLE), and low-stringency Southern analysis demonstrated the absence of

261 High-stringency Southern blots indicate that all five pathoge

262 ed to any walleye cellular gene based on low-stringency Southern blotting.

263 show the effects of an increasing selection stringency strategy and how it results in a tradeoff bet

264 e use of comparative sequence analysis, high-stringency structure prediction, proteolytic susceptibil

265 such that hybridization did not occur (high stringency), suggesting that the presence of the cationi

266 4 and/or 2 kb mRNA expression, even at high stringency (t(m)-8.8 deg. C).

267 d to bioinformatics calculation and P values stringency tests.

268 1) that Qa-2 are peptide receptors of higher stringency than ordinary class I molecules, and 2) that

269 med to a greater extent at a slightly higher stringency than the corresponding mismatched hybrids.

270 sual combination of high avidity and relaxed stringency that RI displays.

271 ivation (enabling ready control of selection stringency), the droplet size can be contracted (to conc

272 ous constraints that explain this mutational stringency, the structure does reveal a hydrophobic pock

273 of discrete RNA molecules hybridize at high stringency to cloned probes prepared from the 28S or 18S

274 e prohead RNA (pRNA) imparts specificity and stringency to the motor by ensuring the correct orientat

275 show that domain II imparts specificity and stringency to the motor during the packaging initiation

276 rains contain a gene that hybridizes at high stringency to the S. dysenteriae type 1 haem receptor ge

277 At lower stringency, up to 97% of annotated genes were identified

278 g a human fetal brain cDNA library under low stringency using cDNA encoding the mouse ligand of Cek5

279 ffinity clones under conditions of identical stringency using fluorescence-activated cell sorting.

280 wed by an overnight hybridization, short low stringency wash and detection of the labeled probe.

281 ted to achieve selectivity without a thermal-stringency wash.

282 Following low-stringency washes and a 2-day exposure, strongly hybridi

283 After stringency washes, the entire long arm of chromosome 6 (

284 ze the effect to the posthybridization array stringency washes.

285 itions for optimal in situ hybridization and stringency washing, the detection of probes and the capt

286 To enhance stringency, we employed RNA silencing alone and together

287 screen a human brain cDNA library under low stringency, we have cloned a homologous cDNA preferentia

288 finding algorithms motif-x and MMFPh at high stringency, we identified compartmentalization of phosph

289 n 200 patterns of length 2 or greater at 99% stringency were found among the 72 genes we compared.

290 Using a high-stringency Western blotting protocol, the anti-1b antibo

291 r similar kinases could influence checkpoint stringency, which may differ among species and between s

292 uence differences is also critical: too much stringency will prevent recombination between different

293 ut B chromosomes by screening DNA at reduced stringency with a B centromeric probe.

294 eened a normal rat liver cDNA library at low stringency with a MDR1 cDNA fragment containing the P-lo

295 nd models by associating alignments of lower stringency with high-quality alignments.

296 A zoo-blot probed at moderate stringency with labeled cDNA revealed a strong conservat

297 Hybridization at low posthybridizational stringency with restriction enzyme-digested human genomi

298 Sequences hybridizing at high stringency with the P. syringae pv. tomato hrpW were fou

299 oute significantly influences the bottleneck stringency, with aerosol transmission imposing greater s

300 We used a high-stringency yeast two-hybrid method to screen a human hea