ライフサイエンスコーパス: structure determination

1 zed by circular dichroism analysis and X-ray structure determination.

2 rge-scale production and NMR-based secondary structure determination.

3 ystallography is the main method for protein structure determination.

4 ues suitable for in meso crystallization and structure determination.

5 n for single-crystal X-ray-diffraction-based structure determination.

6 omain is the only one that has so far eluded structure determination.

7 ligands may promote aaRS crystallization and structure determination.

8 ender them inappropriate for high-resolution structure determination.

9 ion between TNKS1 and USP25 by X-ray crystal structure determination.

10 ity from the viewpoint of single-molecule 3D structure determination.

11 s, resulting in accurate, unbiased, and fast structure determination.

12 erties; it is also particularly suitable for structure determination.

13 rotein sequences far outpaces the ability of structure determination.

14 e predominant method used for macromolecular structure determination.

15 l-diffracting crystals that are suitable for structure determination.

16 ics of proteins--they have not been used for structure determination.

17 patterns, according to single-crystal X-ray structure determination.

18 ains a formidable challenge for nucleic acid structure determination.

19 -chain resonance assignment and unsupervised structure determination.

20 an profiling and, upon use of tandem MS, for structure determination.

21 Geometry Problem (DGP) associated to protein structure determination.

22 llization is a rate-limiting step in protein structure determination.

23 ysics-based, Bayesian framework for improved structure determination.

24 has not been previously utilized for surface structure determination.

25 ut it is still insufficient for explicit ion structure determination.

26 on and the molecular mechanisms of cell wall structure determination.

27 ted previous attempts at crystallization and structure determination.

28 rotein crystals and electron diffraction for structure determination.

29 BSD) is one of the primary tools for crystal structure determination.

30 mbrane protein targets is often required for structure determination.

31 is the main method for experimental protein structure determination.

32 apturing specific target molecules for their structure determination.

33 are frequently used in x-ray crystallography structure determination.

34 remained one of the foremost challenges for structure determination.

35 synthesis served as an important tool in the structure determination.

36 making it a useful tool for cryo-EM protein structure determination.

37 ntional experiments without DNP, for protein structure determination.

38 ampicillin that this can lead to failure of structure determination.

39 us LPL/GPIHBP1 complex that was suitable for structure determination.

40 hy has been the standard technique for virus structure determinations.

41 conformer is not visible in lower resolution structure determinations.

42 thods to generate new tools for nucleic acid structure determinations.

43 undergone a revolution because X-ray crystal structure determinations aided structure-based drug desi

44 collection strategy is suitable for routine structure determination and can be implemented at most m

45 regularity and flexibility make experimental structure determination and computational modeling chall

46 strate that this method is suited to both 3D structure determination and correlative light/electron m

47 of reductant were characterized by molecular structure determination and electron paramagnetic resona

48 As shown by structure determination and in vitro measurements, this

49 s of proteins never observed by experimental structure determination and inaccessible to homology mod

50 resolution, respectively, using cryo-EM for structure determination and mass spectrometry for protei

51 d outline a strategy for the high-resolution structure determination and positioning of isotope-label

52 Using structure determination and solution studies of both dim

53 osphoproteins and demonstrate phosphoprotein structure determination and synthetic protein kinase act

54 Recent advances in structure determination and the development of biophysic

55 now be used to design constructs for protein structure determination and to develop enzyme inhibitors

56 Here we describe the isolation, structure determination, and anti-cancer activity of a c

57 icant advancement in synthetic chemistry, in structure determination, and in the understanding of fac

58 ar Overhauser effect (NOE) experiments; (iv) structure determination; and (v) paramagnetic probe titr

59 Here, we describe an integrative structure determination approach that relies on in vivo

60 We introduce an integrated structure determination approach that simultaneously use

61 By posing the material-structure determination as a multiobjective optimization

62 he use of EM maps to help with X-ray crystal structure determination, as described in this protocol,

63 ardly benefit from the rapid pace of protein structure determination, as most newly solved structures

64 X-ray crystallography allows macromolecular structure determination at both X-ray free electron lase

65 idly emerging as a powerful tool for protein structure determination at high resolution.

66 FX) in lipidic cubic phase (LCP) for protein structure determination at X-ray free-electron lasers (X

67 Methods of protein structure determination based on NMR chemical shifts are

68 ently-developed approach for de novo protein structure determination based on the incorporation of sh

69 The RV-C have been refractory to structure determination because they are difficult to pr

70 ctron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed u

71 bly the most definitive method for molecular structure determination, but the inability to grow suita

72 l boost in successful macromolecular complex structure determination by both X-ray crystallography an

73 An important task of macromolecular structure determination by cryo-electron microscopy (cry

74 Structure determination by cryo-electron microscopy has

75 e conformation and allow for high-resolution structure determination by cryo-electron microscopy.

76 led to this breakthrough in high-resolution structure determination by cryo-EM and point to challeng

77 The recent success in ribosome structure determination by cryoEM has opened the door to

78 a few million protein particles required for structure determination by cryogenic electron microscopy

79 sul crystallization of membrane proteins for structure determination by crystallographic approaches.

80 ectron spectrum, and the previously reported structure determination by electron diffraction.

81 edia using a focused ion beam for subsequent structure determination by MicroED.

82 The use of NOEs as the primary source of structure determination by NMR spectroscopy is time cons

83 Structure determination by NMR spectroscopy showed that

84 ns in proteins is an essential part of their structure determination by NMR spectroscopy.

85 Protein structure determination by proton-detected magic-angle s

86 fferent steps and considerations involved in structure determination by single-particle cryo-EM to pr

87 cromolecular crystals that allows successful structure determination by single-wavelength anomalous d

88 rystals are thousands of times too small for structure determination by synchrotron X-ray diffraction

89 an provide a simple path for high-resolution structure determination by the cryoEM method MicroED and

90 y addressing one of the major bottlenecks to structure determination by X-ray crystallographic method

91 Structure determination by X-ray crystallography and cry

92 obtaining high quality protein crystals for structure determination by X-ray crystallography.

93 to triage the most promising ligands for 3D structure determination by X-ray crystallography.

94 nst Notum in a biochemical assay followed by structure determination by X-ray crystallography.

95 nknown structures, especially in cases where structure determination by X-ray single-crystal analysis

96 has opened the possibility to native de novo structure determinations by the use of intrinsic scatter

97 ugh crystallization from the gas phase, with structure determination carried out directly from powder

98 So far, all protein structure determinations carried out using FELs have bee

99 tegy to enhance the success rates of Fabs as structure determination chaperones.

100 d at the Advanced Photon Source) and ex situ structure determination confirmed the stability and pred

101 Crystal structure determination coupled with basic biochemical a

102 High-resolution structure determination crucially depends on the homogen

103 es significantly enhanced resolution in FRET structure determinations, demonstrated here in a study o

104 aspect of RiPP research is the difficulty of structure determination due to their architectural compl

105 ges reduces the confidence and throughput of structure determination during several steps of data pro

106 larvae protein (Lgl2) that resisted years of structure determination efforts, at 3.2- angstrom resolu

107 The resulting experimental inferential structure determination (EISD) method is size extensive

108 ever, during growth of crystals required for structure determination enzymes are often exposed to con

109 However, structure determination, even using de novo methods, rem

110 Structure determination for amyloid fibrils presents man

111 MR and Raman spectroscopy, and also by X-ray structure determination for CH(3) N(3) .AsF(5) .

112 d refinement has revolutionized the field of structure determination for large biomolecular systems.

113 unanswered question due to the challenges of structure determination for membrane protein complexes.

114 e-residue couplings and demonstrate accurate structure determination for several proteins 6-41 kDa in

115 ple allow researchers to perform X-ray virus structure determination for single particles at room tem

116 CA_C2195 was chosen for crystal structure determination for structure-based function ann

117 individual proteins are sufficient to drive structure determination for the whole complex.

118 The structure determination for this model system shows exce

119 Quaternary structure determination for transmembrane/soluble protei

120 This in turn allows structure determination from crystals much smaller and m

121 as been conclusively demonstrated by crystal structure determination from high-resolution powder diff

122 hod should enable rapid and reliable protein structure determination from near-atomic-resolution cryo

123 ed method that uses electron diffraction for structure determination from very small three-dimensiona

124 on sources it has become possible to perform structure determinations from the measurement of multipl

125 continuous cubic phases for membrane protein structure determination, generation of advanced material

126 The advent of K(+) and Na(+) ion channel structure determination has facilitated numerous investi

127 Crystal structure determination has long provided insight into s

128 Crystal structure determination has revolutionized modern scienc

129 The most informative SSCCs for structure determination, i.e., (3)J, were computed with

130 Most current approaches for structure determination improve cryo-ET resolution eithe

131 This enables high-resolution cryo-EM structure determination in a matter of days on a single

132 Structure determination in complex with hen egg white ly

133 ture exceeds current standards of NMR and EM structure determination in terms of molecular weight and

134 d to virtually all areas of crystallographic structure determination including high-pressure studies,

135 ctron microscopy (cryo-EM), a key method for structure determination involves imaging purified materi

136 Integrative structure determination is a powerful approach to modeli

137 Atomic-resolution structure determination is crucial for understanding pro

138 Despite the advances in NMR, structure determination is often slow and constitutes a

139 c-level structural data, and, generally, NMR structure determination is restricted to small (<30 kDa)

140 Its crystallographic structure determination is the first for a nonheme iron

141 In practice, the primary barrier to structure determination is the preparation of a frozen s

142 range of specimens, single-particle cryo-EM structure determination is transforming structural biolo

143 The most powerful method for protein structure determination is X-ray crystallography which r

144 routinely employed at synchrotrons for novel structure determination, is now possible at X-ray FELs.

145 tional spectroscopy and single-crystal X-ray structure determination, making these compounds the firs

146 We propose a crystal structure determination method that includes experimenta

147 e advances in experimental and computational structure determination methods including macromolecular

148 Two structure determination methods, based on the molecular

149 these superlattices pose a new challenge for structure determination methods.

150 makes them largely inaccessible to standard structure determination methods.

151 uctural level due to limitations in existing structure determination methods.

152 ing is difficult to visualize with classical structure determination methods.

153 technique and place it in context with other structure-determination methods.

154 uration, and epitope mapping of 8H9 based on structure determination, modeling, and yeast display met

155 inst GT-3 NS3/4A protease is elucidated with structure determination, molecular dynamics simulations

156 Protein-structure determination needs a considerable amount of p

157 pectroscopy in solution and by X-ray crystal-structure determination of (Bu4N)2P16 in the solid state

158 X-ray structure determination of (E)-1,2-bis{(E)-2-chloro-1-(c

159 Overall, the results represent the first structure determination of a bacterial diterpene cyclase

160 We report the synthesis and crystal structure determination of a gold nanocluster with 103 g

161 Here, we report the synthesis and total structure determination of a large box-shaped Ag67 nanoc

162 We report the structure determination of a large gold nanocluster form

163 Herein, we report the structure determination of a long unknown phase of glyci

164 We report the synthesis and structure determination of a new Au22 nanocluster coordi

165 ntial applications for the three-dimensional structure determination of a range of objects, ranging f

166 tron microscopy have enabled high-resolution structure determination of AAA+ proteins trapped in the

167 omolecular refinement approaches be used for structure determination of abiological molecular complex

168 Structure determination of all major aminergic subfamili

169 experimental demonstration of single-shot 3D structure determination of an object; in this case, indi

170 By contrast, structure determination of asymmetric components remains

171 Single-crystal structure determination of asymmetric pai-chromophoric b

172 oelectron microscopy for the high-resolution structure determination of biomolecules in solution." Ac

173 to the cells for high-resolution cryo-ET and structure determination of biomolecules in their native

174 Here we used (113)Cd NMR spectroscopy during structure determination of Bud31p, a 157-residue yeast p

175 A recent structure determination of CcdA has provided critical in

176 d with significant success to facilitate the structure determination of challenging macromolecules as

177 on has proven successful for high-resolution structure determination of challenging membrane proteins

178 at the Fritz Haber Institute is employed for structure determination of clean and H(2)O-dosed (111) m

179 algorithm (GA), is a successful approach for structure determination of COF-300 from 3D-ED data.

180 This discovery offers the prospect of facile structure determination of complex biological macromolec

181 community to guide and inform the process of structure determination of complex organic compounds, we

182 Structure determination of covalent organic frameworks (

183 This work combines 3D atomic structure determination of crystal defects with DFT calc

184 We report the first experimental structure determination of dihydroxycarbene (HO-:C-OH),

185 n of these functionally essential states, 3D structure determination of excited states (ESs) of RNA r

186 The approach is parametrized on the crystal structure determination of flutamide, flufenamic acid, a

187 ered a unique mechanism for this through the structure determination of four sequence-diverse M prote

188 Structure determination of free PLK4-PB3 and its STIL-CC

189 Structure determination of gold nanoparticles (AuNPs) is

190 The traditional structure determination of gp41-M-MAT using the Xplor-NI

191 The recent X-ray crystal structure determination of HDAC6 enables an understandin

192 iology and the clinical impact, we aimed for structure determination of IgE and the complex of IgE wi

193 Precise three-dimensional (3D) atomic structure determination of individual nanocrystals is a

194 zed HS-binding protein of interest, putative structure determination of isolated compounds by a hydro

195 The close agreement of NMR secondary structure determination of isolated RNA elements with DM

196 However, structure determination of large complexes and assemblie

197 allography could have an important impact on structure determination of large protein complexes with

198 We describe an approach to the structure determination of large proteins that relies on

199 Crystal structure determination of lithiated N-methylaniline wit

200 Integrative structure determination of macromolecular assemblies req

201 omogram averaging, and this technique allows structure determination of macromolecular complexes in s

202 ogenic electron microscopy (cryo-EM) enables structure determination of macromolecular objects and th

203 ces in electron cryo-microscopy have enabled structure determination of macromolecules at near-atomic

204 th X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-re

205 Recent advances in structure determination of membrane proteins have allowe

206 oligomers opens an exciting new path towards structure determination of membrane-embedded homo- or he

207 experiments are powerful methods for crystal structure determination of microcrystalline powders.

208 X-ray structure determination of monohydrazinium 3,5-bis(dinit

209 Here we have addressed this question by the structure determination of mouse TMEM16A by cryo-electro

210 ets to prevent flavivirus infection, and the structure determination of NS2B will help us to understa

211 Here, we report the ab-initio structure determination of orthocetamol obtained by 3D e

212 This work lays the groundwork for structure determination of other lasso peptides that tar

213 Structure determination of PelB TPRs 8-11 enabled us to

214 e direction of biological cascades,8 and the structure determination of periodically positioned molec

215 The crystal structure determination of photosynthetic protein comple

216 Our work paves the way for high-throughput structure determination of proteins from minimal amounts

217 graphy will become an important tool for the structure determination of proteins that are difficult t

218 ormational flexibility currently hinders the structure determination of several classes of membrane r

219 Our approach provides a paradigm for the structure determination of similarly complex macromolecu

220 strategy that uses macromolecule methods for structure determination of small molecules and their ass

221 adigm shift in the use of NMR active tags in structure determination of small molecules in complex mi

222 Despite recent progress in the structure determination of substrate-bound ABC exporters

223 icroscopy and protein crystallography to the structure determination of subunits of multimeric comple

224 The approach enables structure determination of the 468 kDa large dodecameric

225 Structure determination of the Apo2L/TRAIL-DR5-AMG 655 t

226 eraction to empower cryo-electron microscopy structure determination of the complex at 3.15 angstrom

227 Here we describe the structure determination of the Cry3A toxin found natural

228 nd transcriptional analyses combined with PG structure determination of the guaA mutant enabled us to

229 Structure determination of the HIV-1 envelope glycoprote

230 e engineering of a crystal lattice to enable structure determination of the interaction between these

231 X-ray structure determination of the N170A enzyme in complex w

232 ntal data have been used for the integrative structure determination of the p85alpha dimer.

233 ctively, in facilitating crystallization and structure determination of the Prdm14-Mtgr1 complex, or

234 hemical synthesis and complete spectroscopic structure determination of the putative cross-link remna

235 Structure determination of these complexes in a purified

236 Despite recent progress in structure determination of these transporters, the confo

237 y, kinetic analysis was coupled with crystal structure determination of these WoA variants.

238 The characterization and high-resolution structure determination of this enzyme should aid in its

239 X-ray structure determination of three 11H-indolo[3,2-c]quinol

240 difficulties associated with high resolution structure determination of transmembrane proteins, addit

241 Here we report the structure determination of two different protein samples

242 Structure determination of unexpected products obtained

243 In this vein, we report the synthesis, structure determination, optical properties, and theoret

244 ent with additional experimental restraints (structure determination) or computational methods (struc

245 omics Center for Infectious Disease (SSGCID) structure determination pipeline.

246 addition to continuous rapid progress in RNA structure determination, probing, and biophysical studie

247 The method should streamline the cryo-EM structure determination process, providing accurate and

248 maps are the ultimate result of the cryo-EM structure determination process.

249 DFT) and higher-level methods for electronic structure determination provide valuable quantitative in

250 The structure determinations provide valuable insight, at su

251 d with the same amino-acid sequence used for structure determination, providing a direct link between

252 should also provide useful tools for protein structure determination, refinement, and prediction prog

253 and subsequent affinity improvement without structure determination remain a challenge in the develo

254 Structure determinations reveal M-CO distances ranging f

255 The crystal structure determination revealed a zwitterionic diazoniu

256 X-ray structure determination revealed an NTD consisting of tw

257 X-ray crystallographic structure determination revealed that these monosacchari

258 X-ray structure determination reveals a remarkable "universal

259 A single-crystal X-ray structure determination reveals that O2 binds end-on to

260 ein lattices should enable new approaches to structure determination, sensing, and nanomaterial engin

261 Crystal structure determination shows that the FIP200 CTR contai

262 These examples highlight the challenges in structure determination specific to this class of protei

263 set of particles for input to the downstream structure determination steps.

264 Finally, NMR structure determinations suggested that MTP1 would be or

265 F), along with tools from the macromolecular structure determination suite PHENIX.

266 s are difficult to resolve using traditional structure determination techniques.

267 xperimental method of three-dimensional (3D) structure determination that exploits the increasing eas

268 We include typical workflows developed for structure determination that involve multi-step purifica

269 We report herein an approach to molecular structure determination that relies on a versatile toolk

270 ray FEL data can be used for de novo protein structure determination, that is, without previous knowl

271 its position as the definitive method of MOF structure determination, these issues must be addressed

272 tion of the combination of three-dimensional structure determination through PCDI with a cryogenicall

273 n of specimen preparation methods, assisting structure determination to high resolution with minimal

274 ues with applications ranging from molecular structure determination to human imaging.

275 from fast direct-electron detectors allowed structure determination to near-atomic resolution from 3

276 ion of crystals using the in meso method and structure determination to resolutions ranging from 2.4

277 escence imaging and higher-resolution atomic structure determination, to cover the full scale of cell

278 e maturation of cryo-EM as a high-resolution structure-determination tool, I believe there still are

279 ces in high throughput sequencing, chromatin structure determination, transcription factor and enhanc

280 culties of growing high-quality crystals for structure determination using synchrotron radiation.

281 erential scanning calorimetry (DSC), crystal structure determination, UV-vis absorption, X-ray absorp

282 Experimental structure determination verified that our Rb:hIgG1 model

283 and MCD spectroscopies, single-crystal X-ray structure determination, vibrational spectroscopy, NMR a

284 Historically, room-temperature structure determination was succeeded by cryo-crystallog

285 ing geometry restraints in aiding quaternary structure determination, we introduce two new filters, t

286 ergents frequently used for membrane protein structure determination, were investigated using electro

287 ing its promise for experimental biomolecule structure determination where only semireliable data are

288 was rationalized through an X-ray cocrystal structure determination, which showed a flipped binding

289 pairing mutation enabled crystallization and structure determination, while reaffirming the lack of p

290 was required to get spectra suitable for NMR structure determination, while the tag was required for

291 Overall, faster structure methods for structure determination will serve the natural products

292 es image quality, and allows high-resolution structure determination with a minimum of material and d

293 regime, paving the way to nanometer-scale 3D structure determination with electrons.

294 ant reductive dehalogenase and, by combining structure determination with EPR (electron paramagnetic

295 es an efficient means to augment integrative structure determination with in vivo observations.

296 the tenths of femtosecond time scale enables structure determination with no reduction or X-ray damag

297 to good quality crystals, suitable for X-ray structure determination with unprecedented probability l

298 outlining some key aspects in the process of structure determination, with the goal to encourage nonc

299 k also demonstrates the feasibility of local structure determination within large noncrystalline asse

300 mputationally intensive steps of its cryo-EM structure determination workflow.