ライフサイエンスコーパス: subconductance

1 eyond the transmembrane region, resulting in subconductance.

2 n probability severalfold without inducing a subconductance.

3 potential has little effect on gating of the subconductance.

4 annel opening to the full-size versus a 3-pS subconductance.

5 states referred to as the full conductance, subconductance 1, and subconductance 2 states.

6 the full conductance, subconductance 1, and subconductance 2 states.

7 , p(681-690), and p(681-685) induced similar subconductances and long-lasting channel closings in ske

8 +)) RyRs, cytosolic RR induced a predominant subconductance at a positive but not negative holding po

9 However, analysis of K channel subconductance behavior has suggested a strict coupling

10 Fast closings and overt subconductance behavior of the wild-type I(Ks) channel r

11 ced the RyR1 channel P(o) but did not rescue subconductance behavior.

12 74 of 88 RyR3 channels exhibited pronounced subconductance behavior.

13 el recordings reveal striking, pH-dependent, subconductance behaviors in G178D (or G178E and equivale

14 ts suggest that RR inhibits RyRs and induces subconductances by binding to cytosolic and lumenal site

15 fted to pH approximately 7.8), such that the subconductance channels are relatively quiescent at phys

16 This increase in P(o) for the subconductance channels by alkalinization is not associa

17 Open probability for the subconductance channels can then be increased by intrace

18 the frequency and decrease the open time of subconductance channels, while oxidizing conditions decr

19 o an increase in the occurrence of smaller, 'subconductance' currents with the higher concentrations

20 ite their greater frequency, the duration of subconductance events is so short relative to the main s

21 neither reduced P(o) nor recovered multiple subconductance gating.

22 The major subconductance had a current amplitude of 52% of fully o

23 t, 10 nm to 1 microm sMCa induced long lived subconductances having 48% of the characteristic full op

24 onductance states proportional to pA-induced subconductances (i.e. 43% of pA-modified s1 and s2 subst

25 Lumenal RR induced a major subconductance in cardiac RyR at negative but not positi

26 Cytosolic and lumenal RR also induced subconductances in Ca(2+)-conducting skeletal and cardia

27 not positive holding potentials and several subconductances in skeletal RyR.

28 nce of glow amplitudes parallels that of the subconductances induced by these ryanoids in single-chan

29 (Q10=2.4) drives AMPA channels with multiple subconductances into the higher-conducting states at hig

30 ease in the percent of channel openings to a subconductance level approximately 60% of the maximal si

31 ocus in the pore when the channel opens to a subconductance level as compared to the fully open level

32 o a 23 pS main conductance level and a 19 pS subconductance level, with infrequent openings to a 27 p

33 e single channel data, including that of the subconductance level.

34 availability and channel openings to various subconductance levels (sublevels) as well as the fully o

35 ild-type human potassium channel, long-lived subconductance levels coupled to activation are a key fe

36 ong multiple amplitude levels reminiscent of subconductance levels of ion channels.

37 he approach is applicable to data containing subconductance levels or multiple channels and permits s

38 The underlying single-channel currents show subconductance levels resulting from limitations in inne

39 uency in mdx muscle and reflect occupancy of subconductance levels seen during complete activations.

40 ut mice, MS channels also spend more time at subconductance levels than the fully open state.

41 he wild-type (WT) channel rarely visited the subconductance levels under control conditions.

42 end approximately 60% of the time at smaller subconductance levels, often failing to reach the fully

43 urin altered the amplitude of single-channel subconductance levels, weighted mean unitary current, me

44 increasing access of the channel to several subconductance levels, which has the net overall effect

45 mide analogues with no obvious occurrence of subconductance levels.

46 state and rapid transitions between multiple subconductance levels.

47 lved to channel currents with at least three subconductance levels.

48 channels, with well-resolved non-cooperative subconductance levels.

49 ully open state, while reducing occupancy of subconductance levels.

50 channel showed MS channels fluctuate between subconductance levels.

51 f single-channel gating and produce distinct subconductance levels.

52 partially modified channels exhibit distinct subconductance levels.

53 arations indicated that WT ML1 is a multiple subconductance non-selective cation channel whose functi

54 a main conductance of 37.9 +/- 1.1 pS and a subconductance of 17.8 +/- 0.7 pS, with frequent transit

55 The RR-related subconductances of cardiac RyR showed a nonlinear voltag

56 penings but not for the increase in receptor subconductance opening, thereby supporting the two-bindi

57 Full-size and subconductance openings of both mutant and wild-type cha

58 esence of a nonhydrolyzable ATP analog, with subconductance openings significantly shortened by ATPga

59 orter opening bursts and more frequent brief subconductance openings.

60 cysteines in NBD2 decreased the frequency of subconductance openings.

61 idizing conditions decrease the frequency of subconductance openings.

62 ed gates because of the channels dwelling in subconductance rather than closed states.

63 annel, with low concentrations stabilizing a subconductance state and high concentrations abolishing

64 In addition, a subconductance state at 124 +/- 5 pS was identified.

65 f Piezo1 increases the occupancy of open and subconductance state at the expense of decreased occupan

66 A prominent residual or subconductance state corresponding to 43 +/- 4.2 pS (10

67 The s1 subconductance state does not require either salt bridge

68 The occupancy of this subconductance state may explain the differences in chan

69 hanges in PSAC gating with the addition of a subconductance state not present in wild-type channels.

70 attributed to the formation of a long-lived subconductance state of MscL following channel opening.

71 records were filtered at 50 Hz, a very small subconductance state of murine CFTR was observed that ha

72 has a conductance of 4-7 pS, similar to the subconductance state of the second channel.

73 ar to those of the main open state and lower subconductance state of WT Kir2.1; however, the frequenc

74 +, and driven by ryanodine into a long-lived subconductance state that represented approximately 40 %

75 Kir2.1, but the total duration of the lower subconductance state was 3-5 times longer.

76 The occurrence of the lower subconductance state was reduced in the absence of extra

77 A subconductance state with an amplitude 70% that of the m

78 Average Po for the 2-3-pS subconductance state, estimated from the area under the

79 so by locking channels into the prominent s2 subconductance state, suggesting that the most stable co

80 vestigated whether murine CFTR may open to a subconductance state.

81 It has a high open probability and a subconductance state.

82 en this open state and a approximately 65-pS subconductance state.

83 transition from the fully open state to the subconductance state.

84 contributes to maintaining a stable s2 open subconductance state.

85 12)-diketopyridylryanodine readily induced a subconductance state.

86 probability (P(o)) but gives rise to a ROMK1 subconductance state.

87 tivity for the channels via emergence of the subconductance state.

88 ry rare 13 pS step transitions to one of two subconductance states (26 and 13 pS).

89 nnel fluctuates between two clearly resolved subconductance states (alpha and beta).

90 ymmetry of transitions between the main- and subconductance states - a characteristic of NR1/NR2D-con

91 t observation of blocking events as distinct subconductance states and for the first time demonstrate

92 characteristic pattern of transition between subconductance states and reduced sensitivity to Mg2+ bl

93 cation-selective channel exhibited multiple subconductance states and was blocked by high concentrat

94 Subconductance states are a commonly observed feature of

95 ation and probability of occurrence of these subconductance states but did not greatly alter their re

96 ding of the structural basis for ion channel subconductance states further highlight challenges that

97 sites is not required for ryanodine-induced subconductance states in RyR1.

98 ner and induced the appearance of long lived subconductance states in skeletal RyRs reconstituted int

99 ings predominantly show full-conductance and subconductance states in the presence of 24S-HC and EU16

100 ntee maximal channel conductance and favours subconductance states O1 and O2, with O3 and O4 being ra

101 -) VSMCs, CPA-induced channel currents had 3 subconductance states of 14, 32, and 53 pS.

102 evoked cation channel currents (I(cat)) with subconductance states of about 18, 34 and 51 and 68 pS,

103 These slow conversions among subconductance states of the CFTR channel were affected

104 Subconductance states of the channel may result from alt

105 Importantly, we identify and analyze subconductance states of the channel which were not repo

106 urn control the open probability of discrete subconductance states of the intact ion channel.

107 cause the channel to persist in long-lived, subconductance states or, at high ligand concentrations,

108 sMCa stabilized additional subconductance states proportional to pA-induced subcond

109 ate conductance of 140 +/- 8 pS and multiple subconductance states ranging from < or =10 pS to 60 pS.

110 ound to exhibit more frequent transitions to subconductance states than the native RyR2 channels and

111 Many channels display subconductance states that modulate signalling strength(

112 and the absence of either voltage gating or subconductance states upon D-alanine substitution suppor

113 significant change in the mean open time of subconductance states was observed with increasing negat

114 These subconductance states were also observed with wild type

115 t, at negative potentials, brief sojourns to subconductance states were apparent.

116 In ten of thirty granule cells, clear subconductance states were observed with a mean conducta

117 The magnitudes and distributions of subconductance states were studied in chloride channels

118 The channel has six subconductance states with a maximum conductance of 130

119 nopus oocytes, are characterized by multiple subconductance states with only brief transient openings

120 acterizes partial channel openings, known as subconductance states, and develops a new gating model o

121 RyRs, induced the appearance of long-lasting subconductance states, and markedly slowed the spontaneo

122 corresponding to the main open state and two subconductance states, were identified in WT Kir2.1 chan

123 Calcins target RyRs and induce long-lived subconductance states, whereby single-channel currents a

124 2 showed that four intrinsic, non-stochastic subconductance states, which followed a staircase behavi

125 Subconductance states, which predominate in FKBP12.6-str

126 rate unique profiles of full-conductance and subconductance states, with B-973B producing protracted

127 , mecamylamine and tetracaine induced unique subconductance states.

128 or (CFTR) chloride channel exhibits multiple subconductance states.

129 eptor-channel complex, and showed long-lived subconductance states.

130 cal gating behavior with multiple and stable subconductance states.

131 non-selective cation channel, with multiple subconductance states.

132 ing in leaky, unregulated channels gating in subconductance states.

133 ng between a nonconducting state and various subconductance states.

134 s weakly cation-selective and showed several subconductance states.

135 large increases in P(o), and no evidence of subconductance states.

136 lular Na(+), high conductance, and prominent subconductance states.

137 states may account for the observed multiple subconductance states.

138 single Ca(2+) release channels to prolonged subconductance states.

139 Assuming the ratio of full conductance to subconductance to be the same in the fibers as in bilaye

140 els that open almost exclusively to the 3-pS subconductance, while mutations of cysteines in NBD2 dec