ライフサイエンスコーパス: subgenomic

1 from making less to making more genomes than subgenomic 26S mRNA at late times during infections.

2 Subgenomic analysis of the HCV core gene indicated that

3 ore, IFN-lambda blocked the replication of a subgenomic and a full-length genomic HCV replicon in hum

4 the difference in CyPA dependence between a subgenomic and a full-length replicon of JFH-1 was due,

5 Also, using hepatoma cells that harbor subgenomic and full-length replicons of HCV, we found th

6 n HCV genotype 1b, the effects of psh-274 on subgenomic and full-length replicons were examined, and

7 ng Stem 3 modulate -1 PRF and play a role in subgenomic and full-length RNA synthesis.

8 In subgenomic and genomic analyses of subcellular mRNA part

9 ma) has been shown to inhibit replication of subgenomic and genomic hepatitis C virus (HCV) RNAs in v

10 vely with HCV RNA levels in four independent subgenomic and genomic replicon lines (R = 0.41, P = 0.0

11 Thus, BMV subgenomic and genomic RNA syntheses mutually interfered

12 early and late protein expression as well as subgenomic and genomic RNA transcription, were diminishe

13 t concentrations were observed with both the subgenomic and JFH1-derived infectious HCV models.

14 atoma cells expressing HCV (full-length, BB7-subgenomic, and JFH-1 clone) compared with control HCV-n

15 d the accumulation of protein A and genomic, subgenomic, and template viral RNA.

16 recalcitrant CCV genome as three overlapping subgenomic bacterial artificial chromosomes (BACs).

17 ar mRNAs, reinitiation occurs efficiently on subgenomic bicistronic calicivirus mRNAs, enabling synth

18 imately 5-fold less efficient than that from subgenomic CAT mRNA derived from an IGR-containing MG, b

19 virions, whereas genomic RNA3 (gB3) and its subgenomic coat protein (CP) mRNA (sgB4) are copackaged

20 Full-length HCV and HCV subgenomic constructs corresponding to structural and ea

21 truncated capsid proteins generated from HEV-subgenomic constructs that represent all four viral geno

22 e affected by the differences in genomic and subgenomic contexts, which given the technical difficult

23 g motifs that differ between the genomic and subgenomic contexts.

24 entical to those in BMV, suggesting that the subgenomic core promoter can induce the BMV replicase in

25 enotype 1a) cDNA constructs (full-length and subgenomic), core protein alone, viral RNA, or replicati

26 Thus, analysis of population structure with subgenomic data shows the distinction of hospital and no

27 ted with shorter genomic segments as well as subgenomic DI particles.

28 HIV-1 subgenomic DNA fragments, spanning the long terminal rep

29 at pH 7.2 and 37 degrees C in particles with subgenomic DNA, suggesting that pressure exerted by the

30 ed by the accumulation of virions containing subgenomic DNAs of specific sizes.

31 le L172T, the most impaired mutant, had long subgenomic DNAs originating from both termini, suggestin

32 Xrn1 digestion, leading to the production of subgenomic flaviviral RNA (sfRNA).

33 During infection, pathogenic subgenomic flaviviral RNAs (sfRNAs) are produced by resi

34 The production of subgenomic flaviviral RNAs (sfRNAs) is important for vir

35 Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to p

36 clease Xrn1 for the production of pathogenic subgenomic flaviviral RNAs.

37 uitoes, produce a highly abundant, noncoding subgenomic flavivirus RNA (sfRNA) in infected cells, whi

38 Flaviviruses produce an abundant noncoding subgenomic flavivirus RNA (sfRNA) in infected cells.

39 he PR-2B DENV-2 produced increased levels of subgenomic flavivirus RNA (sfRNA) relative to genomic RN

40 more favorable and relatively more abundant subgenomic flavivirus RNA (sfRNA), a byproduct of host 5

41 ce a 300-500-base long noncoding RNA, termed subgenomic flavivirus RNA (sfRNA), by stalling the cellu

42 The DENV non-structural protein NS4B and subgenomic flavivirus RNA interfere with the RNA interfe

43 Moreover, tick-borne flaviviruses expressed subgenomic flavivirus RNAs that interfere with tick RNAi

44 By sequencing a subgenomic fragment of the HIV-1 envelope from study par

45 Subgenomic fragments comprising a complete provirus were

46 for the genetic subtyping of full-length and subgenomic fragments of HIV-1.

47 eichenowi and Plasmodium gaboni based on the subgenomic fragments.

48 removed both miR-122 binding sites from the subgenomic GBV-B RNAs rendered viral RNA amplification i

49 7 cells supporting autonomous replication of subgenomic GBV-B RNAs.

50 nd to block subgenomic genotype 1b (Luc-1b), subgenomic genotype 1a (Luc-1a), and genomic genotype 2a

51 c genotype 1b clones (SG-1b and Luc-1b), two subgenomic genotype 1a clones (SG-1a and Luc-1a), JFH-1

52 olecule inhibitor, PIK93, was found to block subgenomic genotype 1b (Luc-1b), subgenomic genotype 1a

53 replication and/or HCV RNA levels of the two subgenomic genotype 1b clones (SG-1b and Luc-1b), two su

54 required for HCV replication, we screened a subgenomic genotype 1b replicon cell line (Luc-1b) with

55 equence of 3D(pol), ablated replication of a subgenomic HAV replicon in transfected human hepatoma ce

56 1 and 2'-C-methylcytidine were assayed in a subgenomic HCV replicon assay system and found to be pot

57 unwinding >50% at 2 +/- 1 muM, inhibited the subgenomic HCV replicon at 10 muM, and was not toxic at

58 biosynthesis regulate the replication of the subgenomic HCV replicon in Huh-7 cells.

59 f hepatitis C virus (HCV) replication in the subgenomic HCV replicon system, and its corresponding 5'

60 viral replication capacity, as assessed in a subgenomic HCV replicon system.

61 tic activity and inhibits the ability of the subgenomic HCV replicon to replicate in Huh-7 cells.

62 3A hydrophobic domain, in the context of the subgenomic HCV replicon, was stably maintained throughou

63 epatoma cells that express luciferase-tagged subgenomic HCV replicons (Huh7/HCV replicon cells) or th

64 ith these findings, effective replication of subgenomic HCV replicons as well as production of infect

65 Moreover, HCV infection or subgenomic HCV replicons produced a dramatic increase in

66 pathway in mammals, as has been reported for subgenomic HCV replicons, siRNAs that target Dicer inhib

67 and all but NF 023 inhibited replication of subgenomic HCV replicons.

68 shed light on the intracellular dynamics of subgenomic HCV RNA replication from transfection to stea

69 Additionally, the subgenomic HCV RNA was found to replicate more efficient

70 DDX6 abundance influenced the replication of subgenomic HCV RNAs lacking core sequence, the relevance

71 mpaired or completely ablated the ability of subgenomic HCV RNAs to induce cell colony formation.

72 tion, as well as by transient replication of subgenomic HCV RNAs.

73 otein-encoded defects in HCV by constructing subgenomic HCV transcripts capable of simultaneously exp

74 we directly characterized ex vivo total and subgenomic HCV-specific CD4(+) and CD8(+) T cell respons

75 Huh-7.5 cells harboring subgenomic hepatitis C virus (HCV) replicons or infected

76 is study, we show that replication-competent subgenomic hepatitis C virus (HCV) RNA can be transferre

77 cell-free P3HR-1 virion DNA, confirming that subgenomic het DNA was packaged into infectious particle

78 ant to alpha interferon (IFN-alpha) therapy, subgenomic in vitro self-replicating HCV RNAs (HCV repli

79 tein-Barr virus (EBV) strain P3HR-1 generate subgenomic infectious particles that, unlike defective i

80 used to determine the cellular diversity of subgenomic JFH-1 HCV replicons constitutively expressed

81 We analyzed replication of the subgenomic JFH1 replicon in embryonic fibroblasts and pr

82 In WMoV-infected wheat, subgenomic-length mRNAs of vc sense were detected for ge

83 t and provided evidence for the synthesis of subgenomic-length mRNAs of virus complementary sense.

84 ort to demonstrate that emaraviruses produce subgenomic-length mRNAs that are most likely utilized fo

85 etic variation at the genic, chromosomal and subgenomic levels, and use this information to decipher

86 Massively parallel sequencing of captured subgenomic libraries interrogated 99.8% of targeted nucl

87 foci at chromosomal DSBs and pinpoints their subgenomic locations.

88 replication, we designed and characterized a subgenomic luciferase reporter construct.

89 led to diminished expression from alphavirus subgenomic messages, whereas no dramatic diminution in c

90 Previous studies indicated that a 719-nt subgenomic minigenome (DENV-MINI) is an efficient templa

91 Coronavirus subgenomic mRNA (sgmRNA) synthesis occurs via a process

92 ruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription.

93 nstructural protein that is expressed from a subgenomic mRNA in the cytoplasm of virus-infected cells

94 g from these switches were not templates for subgenomic mRNA synthesis but, rather, ambisense chimera

95 can be used for production of templates for subgenomic mRNA synthesis from the DI RNA.

96 r-containing templates used subsequently for subgenomic mRNA synthesis.

97 tly as the wild type did but had a defect in subgenomic mRNA synthesis.

98 ity of leader-body junction formation during subgenomic mRNA synthesis.

99 trol translation, genomic RNA synthesis, and subgenomic mRNA transcription.

100 virus polymerase with equal efficiency, but subgenomic mRNA was undetectable.

101 he ambisense orientation expressed NSs via a subgenomic mRNA, and two viruses grew to titers only mod

102 bling expression of nsp1 from DI RNA-encoded subgenomic mRNA, DI RNA levels were greatly reduced, but

103 RNA and the transcription and translation of subgenomic mRNA.

104 nd in the otherwise-identical nonreplicating subgenomic mRNA7 (sgmRNA7).

105 uctural protein ORFs from a nested set of 3' subgenomic mRNAs (sg mRNAs), and for most of these ORFs,

106 , to place a 5'-terminal genomic leader onto subgenomic mRNAs (sgmRNAs).

107 t includes the production of a nested set of subgenomic mRNAs (sgmRNAs).

108 ockdown of DHX9 increased the ratio of short subgenomic mRNAs (sgmRNAs); in contrast, DHX9 overexpres

109 It is unclear how viral genomic and subgenomic mRNAs compete with each other for the cellula

110 licate through the use of a 3' nested set of subgenomic mRNAs each possessing a leader (65 to 90 nucl

111 ve-strand RNA viruses generate 3'-coterminal subgenomic mRNAs to allow translation of 5'-distal open

112 nonnative locations, mostly late-transcribed subgenomic mRNAs, in the presence or absence of the nati

113 ptional termination of two S-segment-derived subgenomic mRNAs, which revealed that transcription term

114 ome of the full-length SARS-CoV-2 genome and subgenomic mRNAs.

115 and their structural proteins are encoded by subgenomic mRNAs.

116 ails genome replication and transcription of subgenomic mRNAs.

117 es a template switch during the synthesis of subgenomic negative-strand RNAs to add a copy of the lea

118 sense of genomic RNAs are expressed through subgenomic- or near-genomic-length vc-sense mRNAs.

119 nduced HPV16 late gene expression from HPV16 subgenomic plasmids and from episomal forms of the full-

120 cytidylate in the templates for genomic and subgenomic plus-strand RNA synthesis significantly decre

121 rum of genome/mRNA cleavage because only the subgenomic pol III transcripts are efficiently processed

122 ecent in vivo studies have revealed that the subgenomic promoter (sgp) in brome mosaic bromovirus (BM

123 at the specific recognition of the norovirus subgenomic promoter is through binding by the viral RdRp

124 omic and subgenomic RNAs) and after a second subgenomic promoter resulting in 4-28 Broccoli copies.

125 Old World alphaviruses using a second viral subgenomic promoter to express the transgenes, placed ei

126 compared these with a traditional 3' double subgenomic promoter virus expressing either a large, fir

127 he stem-loop forms the core of the norovirus subgenomic promoter.

128 Huh-7 cells or Huh-7 stably replicating HCV subgenomic protein core through nonstructural protein 3

129 l culture and encode a unique protein in the subgenomic region designated as leader of the capsid pro

130 ickens, bats, and rodents in three conserved subgenomic regions (residues 1 to 452 or 974 to 1534 of

131 ng a panel of canine BAC clones representing subgenomic regions of particular interest.

132 he analysis of RSV full genomes, compared to subgenomic regions, provided more precise estimates of t

133 enes to identify genes required for both HCV subgenomic replication and infectious virus production.

134 ated with inhibition of HCV replication in a subgenomic replication system for a series of non-nucleo

135 e induction of hepatic MRP2 secondary to HCV subgenomic replication.

136 human hepatoma cell line expressing the HCV subgenomic replicon (Huh.8) to evaluate CYP1A1 induction

137 Therefore, we measured genotype 1b subgenomic replicon (sg1b) RNA levels under various IFN-

138 ys that included a luciferase expressing WNV subgenomic replicon and an NS1 capture enzyme-linked imm

139 gged NS5A from cells harboring a replicating subgenomic replicon and analyzed the purified protein by

140 through site-directed mutagenesis of a Con1 subgenomic replicon and through biophysical characteriza

141 we synthesized and transcribed a genotype 4a subgenomic replicon and transfected Huh7-Lunet cells wit

142 ed and showed significant potency in the HCV subgenomic replicon assay (<1 muM) and produced high lev

143 ty in the cell-based hepatitis C virus (HCV) subgenomic replicon assay, by virtue of intracellular co

144 ificant antiviral activity in a dengue virus subgenomic replicon assay.

145 s 1a and 1b polymerase inhibition assays and subgenomic replicon assays.

146 ited more potent anti-HCV activity in an HCV subgenomic replicon cell based assay (EC90 = 1.9, 7.4, a

147 tantly, the same events are triggered by HCV subgenomic replicon cells but not by free virus particle

148 We report that a Con1 chimeric subgenomic replicon containing the NS4B gene from the cl

149 This ability was also shared by a subgenomic replicon derived from the related GB virus B

150 eover, a full-length HCV replicon, but not a subgenomic replicon devoid of the core gene, significant

151 Using the HCV subgenomic replicon expression system, we show that secr

152 he development of a selectable, bi-cistronic subgenomic replicon for bovine viral diarrhea virus (BVD

153 we demonstrate that the expression of an HCV subgenomic replicon in cultured cells results in the acq

154 es in NS5A supported stable replication of a subgenomic replicon in Huh-7.

155 atitis C virus (HCV) genotype 1b strain Con1 subgenomic replicon in human hepatoma cells.

156 sence of other nonstructural proteins of the subgenomic replicon is in part responsible.

157 However, the mutant form of a subgenomic replicon of genotype 3 HEV replicated more ef

158 4 species (DHCR24*) in cells harboring a HCV subgenomic replicon RNA or ectopically expressing NS3-4A

159 that are essential for accumulation of GBV-B subgenomic replicon RNA.

160 es on individual NS4B proteins and on an HCV subgenomic replicon showed that the lipid modifications,

161 ific inhibitor of HCV replication in the HCV subgenomic replicon system (IC(50) = 1.28 microM) with s

162 We used a subgenomic replicon system to assess the effects of the

163 an IC(50) of 0.17 microM in the genotype 1b subgenomic replicon system.

164 tion of hepatitis E virus genotype 3 both in subgenomic replicon systems as well as a full-length inf

165 presses HCV RNA in both infectious virus and subgenomic replicon systems.

166 ase region of a hepatitis C virus (HCV) Con1 subgenomic replicon to ascertain the role of the helicas

167 in Huh7-derived EN5-3 cells harboring an HCV subgenomic replicon with the nonstructural region NS3-NS

168 ontext of the cells transfected with the HCV subgenomic replicon, all except one prevented colony for

169 rface and subsequently examined using an HCV subgenomic replicon, resulting in significant reduction

170 Using a genotype 1b subgenomic replicon, we determined the effect of mutatio

171 The JFH-1 subgenomic replicon, which replicated to high levels in

172 omolar potency against the NS3 protease in a subgenomic replicon-based cellular assay (Huh-7).

173 However, growth of the subgenomic replicon-containing Huh-7.5 cells could be st

174 processing was examined in the context of a subgenomic replicon.

175 re observed with both viral infections and a subgenomic replicon.

176 markers in the context of the full-length or subgenomic replicon.

177 ed NS5A in Huh-7 cells stably expressing the subgenomic replicon.

178 dramatically decreases replication of an HEV subgenomic replicon.

179 culture infectious virus and a corresponding subgenomic replicon.

180 ple hydroxamic acids for inhibition of a HCV subgenomic replicon.

181 anti-HCV activities using genotype 2a JFH-1 subgenomic replicons and infectious virus systems.

182 ure, finally leading to the establishment of subgenomic replicons and the infectious virus model (HCV

183 Chimeric H77 subgenomic replicons containing the entire NS4B gene fro

184 Furthermore, both genotype 1b and 2a subgenomic replicons expressing nonstructural (NS3-5B) p

185 analyzed the impact of SIL on replication of subgenomic replicons from different HCV genotypes in vit

186 udies of drug-selected, cell culture-adapted subgenomic replicons have indicated that an RNA element

187 DNA) library, transfected the cells with HCV subgenomic replicons lacking adaptive mutations, and sel

188 of RNA replication, which was observed using subgenomic replicons of two different genotypes.

189 small interfering RNA in cells carrying HCV subgenomic replicons severely reduced viral replication,

190 ng HCV genotype 1a, 1b, or 2a full-length or subgenomic replicons were resistant to infection with ce

191 Long-term treatment of subgenomic replicons with 13 potently and durably decrea

192 we established efficient RHV-rn1 selectable subgenomic replicons with and without reporter genes.

193 permissive Huh7 cells and three independent subgenomic replicons with various replicative capacities

194 Even with subgenomic replicons, lacking structural viral proteins,

195 by phenotypic screening campaigns using HCV subgenomic replicons.

196 tease domain proteins and in genotype 1b HCV subgenomic replicons.

197 nts and allowed the trans-complementation of subgenomic reporter HEV replicons.

198 ifferent human liver-derived cell lines with subgenomic reporter replicons of HAV as well as of diffe

199 Using subgenomic reporters, as well as HIV replication assays,

200 ed the levels of RNA synthesis directed from subgenomic ribonucleoprotein (RNP) templates.

201 genomic RNAs (RNA1, -2, and -3) and a single subgenomic RNA (RNA4) of Brome mosaic virus (BMV), an RN

202 icistronic open reading frame encoded within subgenomic RNA 7.

203 witching during negative-strand synthesis of subgenomic RNA 7.

204 proteins, but in murine norovirus (MNV), the subgenomic RNA also encodes the VF1 protein, which funct

205 otential cis-acting elements at the start of subgenomic RNA and the 3' end of NV genome for the virus

206 While a West Nile virus (WNV) subgenomic RNA could readily be packaged by structural p

207 he Caliciviridae family of viruses produce a subgenomic RNA during infection.

208 transcription of pregenomic RNA (pgRNA) and subgenomic RNA from the HBV covalently closed circular D

209 Although a subgenomic RNA has been detected in TV-transfected cells

210 ed 6 nucleotides 3' of the start site of the subgenomic RNA in all caliciviruses.

211 etics and affected the levels of genomic and subgenomic RNA in infected cells.

212 Detection of subgenomic RNA is recommended in persistently SARS-CoV-2

213 that functions as the core of the norovirus subgenomic RNA promoter in cells and in vitro.

214 s also found to be a potent inhibitor of HCV subgenomic RNA replication with IC(50) and IC(90) of 40

215 n addition to being potent inhibitors of HCV subgenomic RNA replication, some of the new P(4)-capped

216 uh7 hepatoma cell line that contained an HCV subgenomic RNA replicon and also expressed a GFP-LC3 fus

217 In this report, we demonstrate that a subgenomic RNA replicon of genotype 2a HCV replicated ef

218 autophagosomes from cells that harbor an HCV subgenomic RNA replicon.

219 and demonstrating that BMV can give rise to subgenomic RNA replicons.

220 d virions, despite the great molar excess of subgenomic RNA species that is present in infected cells

221 The full-length genomic and subgenomic RNA strands were detected by Northern blottin

222 otein NTD plays a critical accessory role in subgenomic RNA synthesis and other processes requiring R

223 To date, the mechanism of norovirus subgenomic RNA synthesis has not been characterized.

224 r plastic, structural element in stimulating subgenomic RNA synthesis in coronaviruses.

225 -190 in a way that is critical for efficient subgenomic RNA synthesis in MHV.

226 nserved hexanucleotide sequence required for subgenomic RNA synthesis.

227 99 to N corrected the D41A-induced defect in subgenomic RNA synthesis.

228 SL1 and SL2 are required for subgenomic RNA synthesis.

229 tion may play an important role in directing subgenomic RNA synthesis.

230 ing this deletion are defective in directing subgenomic RNA synthesis.

231 y formed transcription intermediate in viral subgenomic RNA synthesis.

232 en the 5' UTR and the 3' UTR that stimulates subgenomic RNA synthesis.

233 mic RNA in addition to its known function in subgenomic RNA synthesis.

234 ion reduced viral infectivity by attenuating subgenomic RNA synthesis.

235 s at the extreme 3' end of the MHV genome in subgenomic RNA synthesis.

236 e promoter and a short template sequence for subgenomic RNA synthesis.

237 ivities essential for coronavirus genomic or subgenomic RNA synthesis.

238 detected in TV-transfected cells, a separate subgenomic RNA transcript was not required for the initi

239 the BMV replicase in interactions needed for subgenomic RNA transcription in vivo.

240 The subgenomic RNA typically encodes only the major and mino

241 s observed up to 70 days, and of genomic and subgenomic RNA up to 105 days, after initial diagnosis.

242 Subgenomic RNA was diffusely distributed in the cytoplas

243 essed genomic RNA was found to replicate; NV subgenomic RNA was transcribed from genomic RNA by use o

244 gene expression, (ii) a new role for a viral subgenomic RNA, and (iii) a new mechanism for RNA-mediat

245 RNA, polysome association of GYPSY (ATHILA) subgenomic RNA, and transcription via histone H3 lysine-

246 This subgenomic RNA, which was capped, initiated at nucleotid

247 rom genomic RNA and structural proteins from subgenomic RNA.

248 tected full-length replicon RNA and a single subgenomic RNA.

249 ion of viral genome and transcription of the subgenomic RNA.

250 results reveal (i) a new level of control of subgenomic-RNA gene expression, (ii) a new role for a vi

251 ey yellow dwarf virus genomic RNA (gRNA) and subgenomic RNA1 (sgRNA1) is driven by the powerful cap-i

252 cts with the 5' UTRs of both genomic RNA and subgenomic RNA1 via long-distance kissing stem-loop inte

253 A third protein, B2, is translated from a subgenomic RNA3 derived from the 3' end of RNA1.

254 predicted to express from the second ORF in subgenomic RNA3.

255 virions copackage genomic RNA3 (B3) and its subgenomic RNA4 (sgB4).

256 The synthesis of 3' subgenomic RNA4 (sgRNA4) by initiation from an internal

257 t the production of positive- or minus-sense subgenomic RNA7.

258 trast, bovine leukemia virus (BLV) expresses subgenomic RNAP III transcripts that give rise to miRNAs

259 tems consisting of pairs of self-replicating subgenomic RNAs (replicons) derived from tick-borne ence

260 replacement of structural genes, encoded by subgenomic RNAs (SG RNA), with heterologous sequences of

261 ble RdRp binding, accurate initiation of the subgenomic RNAs and efficient RNA synthesis.

262 ated lesser amounts of plus- and minus-sense subgenomic RNAs and spike protein than WT virus.

263 genomic RNA, which resulted in production of subgenomic RNAs as well.

264 mosaic virus (BMV) packages its genomic and subgenomic RNAs into three separate viral particles.

265 However, synthesis of viral genomic and subgenomic RNAs was severely suppressed by UO126 treatme

266 n nsP3 (genomic RNA), the 3'UTR (genomic and subgenomic RNAs) and after a second subgenomic promoter

267 ffect but were competent to synthesize viral subgenomic RNAs, and 8 were not viable.

268 ntirely ablated packaged large quantities of subgenomic RNAs, in addition to genomic RNA.

269 ted in vivo in the absence of detectable TCV subgenomic RNAs, strongly suggesting that the IRES was a

270 Subgenomic RV-A and RV-C RNA replicons failed to amplify

271 transgene in the CNS, suggesting that the DA subgenomic segment can cause cellular dysfunction but no

272 has tamoxifen (Tm)-inducible expression of a subgenomic segment of DA RNA in oligodendrocytes and Sch

273 rmation of the species, whereas asymmetrical subgenomic selection has led to ecotype change.

274 The full-genome and subgenomic sequences identified in our study population

275 ors to determine whether groups of identical subgenomic sequences in the 2 compartments are the resul

276 Through subgenomic sequencing of viruses present in residual pla

277 e chain reaction and genotypes determined by subgenomic sequencing.

278 n the infected hosts: (i) mediation of viral subgenomic (sg) mRNA transcription and (ii) suppression

279 a 3' coterminal nested set of five to eight subgenomic (sg) mRNAs are made that are also 5' cotermin

280 putative viral accessory protein encoded on subgenomic (sg) RNA 7.

281 -infected cells, the genomic (G) RNA and the subgenomic (SG) RNA.

282 and RNAs, a genomic-length RNA (G) RNA and a subgenomic (SG) RNA.

283 ositive-strand RNAs, a genomic (G) RNA and a subgenomic (SG) RNA.

284 voiding packaging of the more abundant viral subgenomic (SG), cellular messenger and transfer RNAs in

285 Using a subgenomic short hair RNA library targeting approximatel

286 ol of virus infection by constructing double subgenomic Sindbis viruses that expressed the mISG15 R15

287 s of full-length anti-MG (aMG) replicate and subgenomic size mRNA for reporter gene expression.

288 a "master circle" chromosome and as numerous subgenomic sublimons that are generated by intramolecula

289 e-strand break in the whole genome and/or in subgenomic targets such as telomere sequences.

290 t capsid expression requires production of a subgenomic transcript, the presence of capsid often bein

291 xed regulatory circuits promoted inefficient subgenomic transcription from inappropriate start sites,

292 ontent and synteny, they have diversified by subgenomic transposon exchanges that equilibrate genome

293 gy for DNA capture that uses PCR products as subgenomic traps.

294 A subgenomic Ty1 mRNA encodes a truncated Gag protein (p22

295 th type 2 JFH-1 HCV or transfection with the subgenomic type 1 HCV replicon.

296 The 3' ends of subgenomic viral mRNAs have been mapped to a stem-loop s

297 sequentially altered the ratio of genomic to subgenomic viral RNA synthesis, promoted recovery of cel

298 sequencing approach, we characterize several subgenomic viral RNAs from human nasopharyngeal specimen

299 binant strains of SINV that have genomic and subgenomic viral RNAs tagged with the Broccoli RNA aptam

300 rols IFN resistance in HeLa cells expressing subgenomic WNV replicons lacking the structural genes.

301 s verified through a de novo RdRp assay on a subgenomic ZIKV RNA template.