ライフサイエンスコーパス: substrate specificity

1 kably different conformational stability and substrate specificity.

2 assembly of LLO is due to the strict enzyme substrate specificity.

3 Disruption of one loop mediates Art1 substrate specificity.

4 Ser(474) phosphorylation as a driver of Akt substrate specificity.

5 ts linkage to a chemical moiety that imparts substrate specificity.

6 le Tyr24, Phe65 and Gln257 contribute to the substrate specificity.

7 structure within substrate in governing the substrate specificity.

8 substitutions in human RR additively affect substrate specificity.

9 y of the transporters accompanied changes in substrate specificity.

10 zation of sequence-level determinants of MMP-substrate specificity.

11 d amino acids are mainly responsible for its substrate specificity.

12 e expanded as the enzyme evolved with higher substrate specificity.

13 amino acid residues of GLUT5 that define its substrate specificity.

14 g its active conformation, thereby effecting substrate specificity.

15 ic active site, protein oligomerization, and substrate specificity.

16 six ceramide synthases (CerS) that differ in substrate specificity.

17 d throughout an active site to alter enzymic substrate specificity.

18 fork and flap structure cleavage by relaxing substrate specificity.

19 y is essential to the acquisition of broader substrate specificity.

20 e amino acid significantly changed the PPO's substrate specificity.

21 constrained our ability to robustly engineer substrate specificity.

22 ich use quality control pathways to maintain substrate specificity.

23 ng that the C-terminal domain has a distinct substrate specificity.

24 oligomerization might lead to differences in substrate specificity.

25 the phosphorylation site play roles in PP2A substrate specificity.

26 iption factors display significantly reduced substrate specificity.

27 ations that increase resistance and/or alter substrate specificity.

28 o the active site plasticity with respect to substrate specificity.

29 translocation by SLC6 transporters of broad substrate specificity.

30 es p53 on Y327, revealing NEK10's unexpected substrate specificity.

31 a K(M) for triuret of 20 muM, and exquisite substrate specificity.

32 on transport kinetics (K(m) and V(max)) and substrate specificity.

33 chromatin, and refining our knowledge of its substrate specificity.

34 hallenges the dogma that E3s alone determine substrate specificity.

35 e site to control both enzyme activation and substrate specificity.

36 e FAD cofactor, with differences observed in substrate specificities.

37 lies possess distinct and often unidentified substrate specificities.

38 he proteasomes have overlapping but distinct substrate specificities.

39 acyl-CoAs with both distinct and overlapping substrate specificities.

40 e number of isozymes with ranges of in vitro substrate specificities.

41 uggesting that these effectors have distinct substrate specificities.

42 of synthetic and lytic enzymes with varying substrate specificities.

43 dicating that these enzymes have contrasting substrate specificities.

44 by changes in subcellular localisations and substrate specificities.

45 monitoring reaction pathways, and profiling substrate specificities.

46 3 and caspase-7, exhibit largely overlapping substrate specificities.

47 SbCYP82D2 is an F8H with high substrate specificity, accepting only chrysin as its sub

48 cupied different functional niches, based on substrate specificity (Acot9 versus Acot2 and -13) and s

49 lance with PABPs and other RBPs with mutable substrate specificity across nucleoplasmic and nucleolar

50 anifested in active-site loops, that dictate substrate specificity across the GH16 evolutionary lands

51 minal insights into its catalytic mechanism, substrate specificity, allosteric regulation, and inhibi

52 Elucidating nuclease substrate specificities and co-factors can support a mor

53 ties have limited our understanding of their substrate specificities and kinetic mechanisms.

54 nding proteins to determine their individual substrate specificities and kinetic parameters.

55 nzymes have relatively broad and overlapping substrate specificities and may hydrolyze a wide range o

56 delineate the evolution of methyltransferase substrate specificities and modification patterns in rRN

57 es have rather narrow but slightly different substrate specificities and that the two exolytic algina

58 tive cytidine deaminases that exhibit unique substrate specificities and thermosensitivities.

59 The substrate specificity and active site structure of bGalE

60 in monolignol biosynthesis, the variation in substrate specificity and activity of CAD can result in

61 By engineering the substrate specificity and activity of the pathway enzyme

62 s the potential signature sequence for their substrate specificity and activity.

63 te to the active-site pocket, potentially in substrate specificity and allosteric regulation.

64 an be divided into three groups according to substrate specificity and amino acid sequence.

65 rine/threonine phosphatases such as PP1 lack substrate specificity and associate with a large array o

66 XAT has broad substrate specificity and can esterify lutein, beta-cryp

67 r characterize the molecular determinants of substrate specificity and catalysis in a new mycobacteri

68 is studies of this loop to probe its role in substrate specificity and catalytic activity.

69 ignature that is potentially responsible for substrate specificity and catalytic selectivity of C4H.

70 howed that the chimeric BCO2 displayed broad substrate specificity and converted carotenoids into two

71 endent of Sec7d, but Sec7d influence the GEF substrate specificity and downstream effector events; 2)

72 the T154Y mutation in SbCCR1 led to broader substrate specificity and faster turnover.

73 The enzyme has a broad substrate specificity and includes mammalian cores 1-8.

74 ew insights into the mechanism of catalysis, substrate specificity and inhibition of SapM, and we ide

75 d our newly developed assay to determine the substrate specificity and inhibitory profile of PLD3 and

76 rthologs, Corallochytrium Csk displays broad substrate specificity and inhibits Src in an activity-in

77 provide a structural explanation for varied substrate specificity and ion transport ratio among CCCs

78 that the T507K substitution alters both SHP2 substrate specificity and its allosteric regulatory mech

79 reviously shown that the N-Srcs have altered substrate specificity and kinase activity compared to C-

80 The kinetic data unravel the so far unknown substrate specificity and mechanism of halide oxidation

81 However, its physiological substrate specificity and mechanism of regulation remain

82 e catalytic cleft play a significant role in substrate specificity and ordered Gag processing.

83 regulatory B-type subunits, which determine substrate specificity and physiological function.

84 onstrated that the KH domain facilitates the substrate specificity and processivity of the DDX43 heli

85 c enzymes in pathways can be used to promote substrate specificity and product composition.

86 To fully characterize substrate specificity and product size of RNase IIIs, we

87 biquitination of FZD, thereby governing USP8 substrate specificity and promoting FZD degradation.

88 iant, PpzA-2, which has also evolved altered substrate specificity and reduced N-methyltransferase ac

89 To investigate separase substrate specificity and regulation, here we develop a

90 The enzyme exhibited a high degree of substrate specificity and selectively generated the 3'-n

91 erform C-H hydroxylation exhibit exceptional substrate specificity and site-selectivity, often throug

92 f of principle, we recapitulate the ligase's substrate specificity and successfully predict how to co

93 ndary metabolism protein architecture guides substrate specificity and that gene duplication and doma

94 PKD2 increases turnover for Syntide-2, while substrate specificity and the role of PKD2 in NF-kappaB

95 veal several structural determinants of LPMO substrate specificity and underpin the notion that produ

96 o acid change in PmxA was found to reprogram substrate specificity and was applied to predict the pre

97 Its substrate-specificity and tissue-specific expression mak

98 our work), revealed three previously unknown substrate specificities, and assigned a function to 25 s

99 teins, engineer enzymes to achieve a desired substrate specificity, and develop drugs with improved s

100 e important insights into caspase/granzyme B substrate specificity, and facilitate the discovery of n

101 identified, harboring different composition, substrate specificity, and in some cases, modes of regul

102 reactions with remarkable rate acceleration, substrate specificity, and product selectivity.

103 of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive

104 tural evolution leading to improved or novel substrate specificities are not wholly defined.

105 mTORC1) and 2 (mTORC2), whose activities and substrate specificities are regulated by complex co-fact

106 Mechanisms of the altered 15-LO substrate specificity are enigmatic.

107 In contrast, defects in substrate specificity are not rescued in the double muta

108 Os, the molecular determinants driving broad substrate specificity are still not easily predictable.

109 Protein ligases of defined substrate specificity are versatile tools for protein en

110 APC/C substrate specificity arises from binding of short degro

111 s spectrometry, we unambiguously defined its substrate specificity as mono(ADP-ribosyl)ated aspartate

112 es in gene expression, protein function, and substrate specificity as the evolutionary bases of gluco

113 e binding pockets, consistent with the broad substrate specificity, as determined by degradomic assay

114 able promiscuity for orthologs with distinct substrate specificity, as illustrated by swapping enzyme

115 aspases and separases have evolved different substrate specificities associated with their specialize

116 essing AtNAA50 revealed conservation of NatE substrate specificity between plants and humans.

117 trates, potentially reflecting divergence in substrate specificity between the metalloprotease domain

118 e conserved Arg-215 plays a critical role in substrate specificity, binding orientation, and active s

119 ss to the adenosine pocket can contribute to substrate specificity but is not the sole determinant.

120 of the substrate-binding site, which confers substrate specificity by concerted conformational change

121 Apart from resolving the substrate specificity conflict through direct in vitro e

122 r results suggest that SHP2/T507K's shift in substrate specificity coupled with its preferential asso

123 T displayed significant differences in their substrate specificities, demonstrating that even modest

124 hat AA9 LPMOs can display different apparent substrate specificities dependent upon both productive p

125 different molecular scissors with different substrate specificities, depending on which of six Tspan

126 the purine salvage pathway exhibit exquisite substrate specificity despite the chemical similarity of

127 uggests cation-pai interactions as the major substrate specificity determinant, which was verified us

128 ructural analysis, we could track changes in substrate specificity during ADP-dependent kinase evolut

129 rovided further evidence of largely distinct substrate specificities, even though some inhibitory cro

130 taphylococcus aureus, ClpP associates to the substrate specificity factors, ClpX and ClpC forming two

131 ipated in multidrug efflux, with overlapping substrate specificities for TolC and FtlC and a distinct

132 amber suppressors, and evolve new amino acid substrate specificities for two pairs.

133 lation function and determined that PatB has substrate specificity for bioorthgonal short N-acetyl cy

134 ce for Cho over Etn, CEK3 and CEK4 had clear substrate specificity for Cho and Etn, respectively.

135 cysteine protease legumain exhibits a strict substrate specificity for cleavage after asparagine and

136 of acyl-Coenzyme A's to alkanes with strict substrate specificity for compounds containing more than

137 T1 (SLC22A6) and OAT3 (SLC22A8) have similar substrate specificity for drugs, but it is far from clea

138 most informative and relevant for predicting substrate specificity for each individual kinase family.

139 Thereby we determined rate and substrate specificity for each single subunit without in

140 SbCCR1 and other CCRs likely confers strong substrate specificity for feruloyl-CoA over other cinnam

141 SbCYP82D1.1 has broad substrate specificity for flavones such as chrysin and a

142 omo sapiens NAD(+) synthetase (hsNadE) lacks substrate specificity for glutamine over ammonia and dis

143 We elucidated the substrate specificity for HGFA using positional scanning

144 otif has been identified and used to explain substrate specificity for PKCalpha, it does not inform t

145 To date, the SUMO E3 ligase that provides substrate specificity for SUMO2-PCNA conjugation in resp

146 anisms in Dcp2 also result in a shift of the substrate specificity from bacterial to eukaryotic mRNA.

147 redict adenylate-forming enzyme function and substrate specificity from protein sequences.

148 how how gene loss can drive the evolution of substrate specificity from retained enzymes.

149 the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransfe

150 Furthermore, EYA1's substrate specificity has remained elusive.

151 ry forces that cause an enzyme to change its substrate specificity; however, these processes remain l

152 Currently used MTGs have low substrate specificity, impeding their biotechnological u

153 are proposed to explain the limitation of AP substrate specificity imposed by the acylation state of

154 we showed that these four CEKs have distinct substrate specificities in vitro.

155 nt was active in vitro but displayed altered substrate specificity in cultured cells, consistent with

156 of the drivers of the evolution of divergent substrate specificity in enzymes with identical active s

157 lays a dramatic reversal of beta-elimination substrate specificity in favor of l-serine over the norm

158 he LP docking motif, which determines G1-CDK substrate specificity in fungi.

159 To gain additional insight into substrate specificity in LPMOs, here we generated a muta

160 NPF6 proteins exhibit different substrate specificity in plants and regulate nitrate tra

161 ng mechanism is likely to be a source of the substrate specificity in plants.

162 mechanism to modulate annealing activity and substrate specificity in various bacteria.

163 al protein-tyrosine phosphatases can exhibit substrate specificity in vivo by combining intrinsic enz

164 binding site in NvTRPM2 with respect to its substrate specificity, in comparison to the classical AD

165 annel may serve as major determinants of the substrate specificity, including a glutamate residue at

166 Through a family-wide characterization of substrate specificities, interactomes and localization,

167 is known about how evolution of transporter substrate specificities is linked to emergence of substr

168 Substrate specificity is achieved by a cluster of three

169 How LF achieves such restricted substrate specificity is not understood.

170 of cellular functions; however, the extended substrate specificity is still unknown for many of these

171 yme's stability and function, and especially substrate specificity, is lacking.

172 s position modulate inhibitor efficiency and substrate specificity leading to drug resistance is uncl

173 ubiquitin ligases and, thus, regulate their substrate specificity, ligase activity, and subcellular

174 PP2A substrate specificity, localization, and regulation by s

175 action or subtle differences within the same substrate specificity, matching the hybrid character of

176 bstrate transport that reveals an allosteric substrate specificity mechanism.

177 Proper annotation and knowledge of substrate specificity mechanisms in this family are high

178 f single-stranded DNA (ssDNA), we probed the substrate specificity, mutation spectra and signatures a

179 tion of this oil is strongly impacted by the substrate specificities of acyltransferases involved in

180 n detailed information on mode of action and substrate specificities of alginate lyases.

181 tes of approximately 1-80 nt/min and exhibit substrate specificities of approximately 0.1-5-fold for

182 rometry, allowing one to rapidly analyze the substrate specificities of chitosan hydrolases that can

183 as novel biochemical tools to determine the substrate specificities of endogalactanases.

184 sequences that can bind to Gid10 showed that substrate specificities of Gid10 and Gid4 overlap but ar

185 despite their importance, the functions and substrate specificities of most Microsporidia transporte

186 lgorithms have been developed to predict the substrate specificities of nonribosomal peptide syntheta

187 for using protein engineering to reconfigure substrate specificities of RNA-modifying enzymes and cov

188 s the expression, tissue-specific roles, and substrate specificities of the different mammalian AOX e

189 Altering the RNA substrate specificities of the enzymes that install thes

190 , and similar experiments confirmed that the substrate specificities of the human orthologs of caspas

191 e aglycones released varied depending on the substrate specificities of the pectinolytic preparation,

192 re experiments to compare the activities and substrate specificities of these effectors.

193 strates and advance our understanding of the substrate specificities of these enzymes in biomass conv

194 The DNA substrate specificities of these Nei-like enzymes imply

195 The catalytic mechanism and substrate specificity of a major PAL from sorghum (Sorgh

196 k establishes the capability of changing the substrate specificity of a protease at many positions in

197 The broad substrate specificity of acyltransferase CT775 provides

198 racterize fully the enzymatic parameters and substrate specificity of ADIPORs.

199 The substrate specificity of AnGDH towards glucose was inves

200 The superior substrate specificity of AnGDH was also demonstrated in

201 To understand the substrate specificity of APN for the development of targ

202 However, the molecular mechanism underlying substrate specificity of APRT and catalysis in both dire

203 eal key residues with roles in catalysis and substrate specificity of Cd-SrtB.

204 Active site architecture and substrate specificity of Cdc14 from the model fungus Sac

205 The extended substrate specificity of CELA3A and CELA3B was comparabl

206 The catalytic mechanism and substrate specificity of cinnamoyl-CoA reductases from s

207 The substrate specificity of ClbL strongly supports a role f

208 y in various clinical settings, in which the substrate specificity of CRBN is altered via the binding

209 tor pair that can be used to investigate the substrate specificity of cysteine proteases, serine prot

210 To explain the differences in substrate specificity of desulpho (ds)-Gl SOTs and to un

211 The substrate specificity of FlmG is conferred by its N-term

212 Here, we revisited the substrate specificity of FUT8 by examining its in vitro

213 The substrate specificity of FUT8 toward bi-antennary N-glyc

214 fer from Cbl to the substrate as well as the substrate specificity of GenK.

215 rovide insight on a molecular level into the substrate specificity of hGGT1 and provide an explanatio

216 thesis were systematically studied about the substrate specificity of human Sulf-1 employing the fluo

217 ing lipoate utilization relies on the strict substrate specificity of LplJ, determined by charge comp

218 We therefore investigated the substrate specificity of Mag1 and found that it excises

219 on the MCD structure, we were able to shift substrate specificity of MCD toward succinyl-CoA through

220 involved alpha-l-amino acids, reflecting the substrate specificity of natural aminoacyl-tRNA syntheta

221 op new tools and probe the plasticity of the substrate specificity of one of these enzymes, we examin

222 mutations of key residues indeed reverse the substrate specificity of OprO to OprP and support the vi

223 me kinetics to investigate the mechanism and substrate specificity of PPEP-1.

224 Characterizing the substrate specificity of protease enzymes is critical fo

225 Switching of the substrate specificity of protein tyrosine phosphatase N1

226 and asparagine-176 may account for the broad substrate specificity of PviPRX9.

227 The unique substrate specificity of ScCdc14 was invariant in homolo

228 Because the subcellular localization and substrate specificity of the catalytic subunit of protei

229 s spectrometry was developed to identify the substrate specificity of the Drosophila melanogaster P-T

230 e that SteE converts both the amino acid and substrate specificity of the host pleiotropic serine/thr

231 taining substrate receptors, determining the substrate specificity of the ligase.

232 ity for pERK2 over ERK2 and to reprogram the substrate specificity of the same DARPin towards non-cog

233 st diversity in metabolites and the matching substrate specificity of their transporters, little is k

234 We profile substrate specificity of these enzymes by employing a sm

235 Understanding the substrate specificity of these enzymes could create oppo

236 In order to elucidate the mode of substrate specificity of these enzymes, we characterized

237 bout the structure, mechanism of action, and substrate specificity of these enzymes.

238 have limited knowledge of the structure and substrate specificity of these secreted enzymes.

239 n ADO and fill a gap in our knowledge of the substrate specificity of thiol dioxygenases.

240 We characterize the substrate specificity of this evolved enzyme, revealing

241 The substrate specificity of this pump matched SOX17-induced

242 The substrate specificity of this pump matched that of SOX17

243 re, we describe the structure, function, and substrate specificity of three tailspike proteins of bac

244 The substrate specificity of TNMT enzymes appears to arise f

245 cture-based protein engineering to alter the substrate specificity of uridine-cytidine kinase 2 (UCK2

246 st method for measuring the average rate and substrate specificity of XNA polymerases in a standard q

247 cture-based insights into the regulation and substrate specificity of ZAP-70, and then we review nove

248 n a soluble monovalent substrate and similar substrate specificities on a glycan array.

249 (FlhB/SctU) functions to "switch" secretion substrate specificity once the growing hook/needle reach

250 ubgroup of PUX proteins, which determine the substrate specificity or affect the ATPase activity of C

251 ynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new bios

252 endopeptidases of disparate architecture and substrate specificity owing to several potential target

253 In this study we characterized the extended substrate specificity (P4-P1) of the NSP cathepsin G usi

254 op, spans the active site and contributes to substrate specificity pockets underpopulated in other HP

255 Curiously, despite their mutually exclusive substrate specificities, PON1 and diisopropyl fluorophos

256 ate that mycobacterial EHs may have a narrow substrate specificity providing a potential explanation

257 ely related UGT76G1 isoforms differ in their substrate specificities, providing new insights into mec

258 well-characterized in terms of function and substrate specificity, regulation across most thioestera

259 strates, the structural determinants of LPMO substrate specificity remain largely unknown.

260 However, how m(6)A writers attain substrate specificities remains unclear.

261 atalytic activity; however, its influence on substrate specificity remains unclear.

262 However, the structural basis for FUT8 substrate specificity remains unknown.Here, using variou

263 D-2, which has the opposite (S)-enantiomeric substrate specificity, reveal the structural basis for s

264 Investigation of their substrate specificities revealed that all these LPMOs ar

265 Studies on the substrate specificity revealed that the alpha1,6-fucosid

266 om oil to reaction water droplets to perform substrate specificity screening of eight model enzymes f

267 hCCS substrate specificity, segregation between solvent and b

268 This enzyme family comprises three substrate specificities; specific phosphofructokinases (

269 iling of proteases that share highly similar substrate specificity spectrums.

270 addition to reusability, thermal stability, substrate specificity, stereoselectivity and rapid catal

271 Substrate specificity studies of NmW CPS polymerase (NmS

272 eblon ubiquitin ligase complex and alter its substrate specificity such that it targets the IKZF1 and

273 PG hydrolytic enzyme with a hitherto unknown substrate specificity that contributes to expansion of t

274 ts of 12 Mg(2+)-dependent 3'-end RNases with substrate specificity that is mostly unknown.

275 e molecules studied has distinct patterns of substrate specificity that likely underlie the clinical

276 or the new ASs, we observe largely invariant substrate specificity that would facilitate the transiti

277 t not only the two pumps have nonoverlapping substrate specificities, their inactivation leads to spe

278 ted whether CELA3A and CELA3B evolved unique substrate specificities to compensate for the loss of CE

279 glycosylase (TGT) homologs that have changed substrate specificities to directly salvage q, mimicking

280 Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic path

281 We find that the SNF2 domain couples substrate specificity to an ATPase step essential for DN

282 ight bioluminescent reporter with orthogonal substrate specificity to firefly luciferase (FLuc) and i

283 specific targeting determinants that impart substrate specificity to rMtases.

284 omparative study of structure, sequence, and substrate specificity to track the evolution of specific

285 Moreover, Vv2KGR exhibited broad substrate specificity toward glyoxylate, pyruvate, and h

286 recombinant OsCAldOMT1 displayed comparable substrate specificities towards both 5-hydroxyconiferald

287 ociated with the gut membrane and has narrow substrate specificity towards the benzenic glucosinolate

288 th the idea that SUMOylation may govern PIM1 substrate specificity under certain contexts.

289 Here we reconstitute and dissect the substrate specificity underpinning the cytoplasmic O-gly

290 method for quantitatively describing kinase substrate specificity using an unbiased peptide library-

291 rstand the mechanisms underlying its relaxed substrate specificity, we determined the crystal structu

292 To examine LsdA's substrate specificity, we heterologously produced the di

293 many S-acyl transferases appear to have lax substrate specificity, we propose that many receptor-lik

294 s-specific differences in both structure and substrate specificity were also identified that may be i

295 tructural insights into the determination of substrate specificity were complemented by mutagenesis s

296 interaction, the mechanism of catalysis and substrate specificity, which facilitate rational design

297 equence homology, inhibition sensitivity and substrate specificity with CDK family members.

298 65 A resolution, and we compare its in vitro substrate specificity with those of fungal Icp55 enzymes

299 itin ligases are the primary determinants of substrate specificity within the ubiquitin-dependent pro

300 id and accurate prediction of their extended substrate specificity would also aid in the design of cu