ライフサイエンスコーパス: sucrose density gradient

1 and eventually to monomers as separated on a sucrose density gradient.

2 lex that sedimented at approximately 5S in a sucrose density gradient.

3 so be detected by co-sedimentation through a sucrose density gradient.

4 isolated between 1.17 and 1.21 g/cm(3) in a sucrose density gradient.

5 remains bound to liposomes centrifuged on a sucrose density gradient.

6 was detected only in the CRD fractions of a sucrose density gradient.

7 by centrifugation and further purified by a sucrose density gradient.

8 t detergent treatment, using a discontinuous sucrose density gradient.

9 se (RT) activity that banded at 1.15 g/ml in sucrose density gradients.

10 tudy (<380 nM), as judged by its mobility in sucrose density gradients.

11 nit that is immature and migrates at 45 S in sucrose density gradients.

12 insoluble protein recovered at the bottom of sucrose density gradients.

13 lexes toward the uncoiled lariat position in sucrose density gradients.

14 pulina hyodysenteriae by using discontinuous sucrose density gradients.

15 R1 by flotation of CHAPS lysates of cells in sucrose density gradients.

16 and comigrates with the outer dynein arm in sucrose density gradients.

17 d with non-polysomal, but dense fractions on sucrose density gradients.

18 lipid membranes as assessed by floatation in sucrose density gradients.

19 tionic lipid was confirmed and quantified on sucrose density gradients.

20 ts with known cytoplasmic dynein proteins in sucrose density gradients.

21 icated by aqueous two-phase partitioning and sucrose density gradients.

22 containing a known glycoprotein in flotation sucrose density gradients.

23 atalytically active following isolation from sucrose density gradients.

24 cofractionates with the outer dynein arm in sucrose density gradients.

25 M Na(2)CO(3) buffer and fractionated through sucrose density gradients.

26 associated with liposomes and aggregated on sucrose density gradients.

27 5 M KI, and copurifies with radial spokes in sucrose density gradients.

28 and fractionation of retinal lysates, using sucrose density gradients.

29 of 1.07 to l.14 as defined by flotation into sucrose density gradients.

30 ent; cores were isolated by sedimentation in sucrose density gradients.

31 o-sedimenting with caveolin and flotillin on sucrose density gradients.

32 Sucrose density gradient analyses revealed formation of

33 Sucrose density gradient analyses revealed that depletio

34 Sucrose density gradient analysis demonstrated that both

35 Sucrose density gradient analysis detected AP1B predomin

36 Sucrose density gradient analysis indicates that a signi

37 Sucrose density gradient analysis of in vitro translates

38 Furthermore, sucrose density gradient analysis revealed significantly

39 Sucrose density gradient analysis revealed that in antim

40 d with normal prostate epithelial cells, and sucrose density gradient analysis showed co-sedimentatio

41 Sucrose density gradient analysis showed that 96% of the

42 High-resolution sucrose density gradient analysis showed that, while mut

43 Sucrose density gradient analysis was employed to determ

44 -free cytoplasmic complexes that copurify in sucrose density gradients and are stable in nonionic det

45 into the vesicle compartment as confirmed by sucrose density gradients and confocal immunofluorescent

46 as isolated as a low buoyant density band on sucrose density gradients and exhibited an increase in l

47 LC9 copurifies with outer arm dynein in sucrose density gradients and is missing only in those s

48 fferent PCDH15 and VLGR1 variants along with sucrose density gradients and the use of vesicle traffic

49 shift from middle to low density regions on sucrose density gradients) and cytosol fractions.

50 resh bovine brain and size fractionated on a sucrose density gradient, and a size-fractionated bovine

51 Yeast lysates were separated on sucrose density gradients, and fractions assayed for org

52 Deriphat-polyacrylamide gel electrophoresis, sucrose density gradients, and isolated PSII particles,

53 GN/endosome-resident SNAREs cofractionate in sucrose density gradients, and show similar solubility o

54 ist in membranes that float on discontinuous sucrose density gradients, and that methyl-beta-cyclodex

55 is found in the same fraction as 125I-Tf on sucrose density gradients, and this fraction can be spec

56 hia coli TonB was found to be distributed in sucrose density gradients approximately equally between

57 ibosome profiles upon centrifugation through sucrose density gradients, association of mutant 30 S su

58 high-speed centrifugation and sedimented in sucrose density gradients at the same bouyant density as

59 In sucrose density gradients, Bop1 from nuclear extracts co

60 y Triton insoluble membranes that floated in sucrose density gradients but was recruited to these mem

61 h these proteins and comigrated with them on sucrose density gradients, but it did not colocalize, co

62 ower sedimentation rate than native virus on sucrose density gradients, but the particles retained al

63 ndicated by immunofluorescence localization, sucrose density gradients, cell fractionation, and yeast

64 However, the splitting was observed with sucrose density gradient centrifugation (SDGC) without I

65 Using sucrose density gradient centrifugation and antibody pul

66 Instead, sucrose density gradient centrifugation and electron mic

67 ner and outer membranes were fractionated by sucrose density gradient centrifugation and identified b

68 wing both ATP-sensitive microtubule affinity/sucrose density gradient centrifugation and immunoprecip

69 ocytosis" into the light vesicle fraction in sucrose density gradient centrifugation assays, as did t

70 ecipitation, native gel electrophoresis, and sucrose density gradient centrifugation assays.

71 Cross-linking sucrose density gradient centrifugation demonstrated an

72 Immunoblotting of rafts isolated by sucrose density gradient centrifugation demonstrated rec

73 Sucrose density gradient centrifugation of a maize mitoc

74 Sucrose density gradient centrifugation of A6 cell deter

75 onocytogenes 100S ribosomes were observed by sucrose density gradient centrifugation of bacterial ext

76 Viral core-like complexes were isolated by sucrose density gradient centrifugation of detergent-tre

77 to ET-1 in subcellular fractions obtained by sucrose density gradient centrifugation of human umbilic

78 Sucrose density gradient centrifugation of large ribosom

79 By sucrose density gradient centrifugation of membranes, ho

80 gent-insoluble membrane fraction prepared by sucrose density gradient centrifugation of postnuclear f

81 Sucrose density gradient centrifugation of postnuclear s

82 Similarly, sucrose density gradient centrifugation of purified MAO

83 Sucrose density gradient centrifugation of the culture m

84 Sucrose density gradient centrifugation of the ribosomal

85 Discontinuous sucrose density gradient centrifugation revealed NET in

86 Characterization of the circulating mRNAs by sucrose density gradient centrifugation revealed that th

87 Examination of lipid rafts isolated by sucrose density gradient centrifugation revealed the con

88 Sucrose density gradient centrifugation reveals two majo

89 se of internal ribosome entry mechanisms and sucrose density gradient centrifugation showed that BC1-

90 Sucrose density gradient centrifugation showed that expo

91 Separation of purified E1 proteins by sucrose density gradient centrifugation showed that the

92 Using sucrose density gradient centrifugation to analyze ribos

93 dily detected in outer membranes produced by sucrose density gradient centrifugation, but it is sarco

94 Mutant viral particles, purified by sucrose density gradient centrifugation, had low infecti

95 Sucrose density gradient centrifugation, immunoblot, and

96 Enzymatic assays, sucrose density gradient centrifugation, immunoprecipita

97 nd when subjected to size-exclusion HPLC and sucrose density gradient centrifugation, in the presence

98 fferent approaches including gel filtration, sucrose density gradient centrifugation, pull-down of di

99 Using sucrose density gradient centrifugation, this study eval

100 pheroplasting and osmotic lysis, followed by sucrose density gradient centrifugation, which separated

101 As shown by sucrose density gradient centrifugation, WT gamma-PAK, S

102 enriched membranes with DodGlc2, followed by sucrose density gradient centrifugation, yielded a super

103 s after disruption and initial separation by sucrose density gradient centrifugation.

104 e steps of gel filtration chromatography and sucrose density gradient centrifugation.

105 eolae-enriched membrane fractions as seen by sucrose density gradient centrifugation.

106 um carbonate without detergent), followed by sucrose density gradient centrifugation.

107 cholesterol were incubated and subjected to sucrose density gradient centrifugation.

108 Oocysts were initially isolated by sucrose density gradient centrifugation.

109 bidopsis suspension-cultured cells following sucrose density gradient centrifugation.

110 ciated with centrosome fractions isolated by sucrose density gradient centrifugation.

111 ce of solubilized oligomers was confirmed by sucrose density gradient centrifugation.

112 response to insulin binding as determined by sucrose density gradient centrifugation.

113 n-insoluble membranes then were separated by sucrose density gradient centrifugation.

114 ans of their light buoyant densities through sucrose density gradient centrifugation.

115 Sucrose density-gradient centrifugation showed that the

116 wever, we show by chemical cross-linking and sucrose density-gradient centrifugation that in the abse

117 amination by aqueous two-phase partitioning, sucrose density-gradient centrifugation, and immunoelect

118 les, as determined by immunoprecipitation or sucrose density-gradient centrifugation.

119 roteins by size exclusion chromatography and sucrose-density gradient centrifugation revealed that th

120 performed gel filtration chromatography and sucrose density gradient centrifugations in H(2)O and D(

121 We have used immunogold localization and sucrose density gradient cosedimentation analyses to con

122 Subcellular fractionation using equilibrium sucrose density gradients demonstrated decreased hyperph

123 Result of the sucrose density gradient experiment suggests that Der in

124 Upon sucrose density gradient flotation, membrane- associated

125 Sucrose density gradient fractionation and gel filtratio

126 Discontinuous sucrose density gradient fractionation and immunoconfoca

127 During sucrose density gradient fractionation of bovine retinas

128 Using sucrose density gradient fractionation of cell membranes

129 Sucrose density gradient fractionation of cytoplasmic ex

130 Using discontinuous sucrose density gradient fractionation of post-nuclear s

131 f epitope-tagged versions of Upf proteins by sucrose density gradient fractionation of soluble lysate

132 Sucrose density gradient fractionation of the culture me

133 Sucrose density gradient fractionation of the purified c

134 Sucrose density gradient fractionation reveals that loss

135 th Gpa1 at the plasma membrane, as judged by sucrose density gradient fractionation.

136 n blot analysis of cell lysates separated by sucrose density gradient fractionation.

137 Using a combination of sucrose density-gradient fractionation-coupled proteomic

138 Sucrose-density gradient fractionation demonstrated that

139 form of CD39L4 by measuring the activity of sucrose density gradient fractions of monomers and parti

140 asts, as well as subcellular fractionations, sucrose density gradients, immunocytochemical labeling,

141 at Pixie associates with the 40 S subunit on sucrose density gradients in an ATP-dependent manner.

142 FP-modified Rab5 as a larger mass complex on sucrose density gradients indicates that it binds to oth

143 ediments with the product of the LF1 gene in sucrose density gradients, indicating that these protein

144 In sucrose density gradients, KIF3C sediments at two distin

145 Its distribution on sucrose density gradients matched the absorbance profile

146 radiolabeling with [(3)H]palmitic acid, and sucrose density gradient membrane partitioning studies.

147 We used sucrose density gradients of nucleosomes prepared by par

148 65 recognized a protein of roughly 65 kDa in sucrose density gradient-purified HHV-7 preparations; tr

149 al detergent extraction and fractionation in sucrose density gradients revealed TcdB-induced redistri

150 as released from infected cells; analysis on sucrose density gradients revealed that the precursor se

151 ntact sites were analyzed using a continuous sucrose density gradient, revealing an apparent heteroge

152 Sedimentation in sucrose density gradients reveals that large unilamellar

153 Sucrose density gradient sedimentation analysis indicate

154 cupied ER complex shows distinct behavior by sucrose density gradient sedimentation analysis.

155 ere expressed in COS-1 cells and analyzed by sucrose density gradient sedimentation and gel filtratio

156 Two-phase partitioning and sucrose density gradient sedimentation established that

157 We used sucrose density gradient sedimentation to ascertain whet

158 on X-100 in combination with gel filtration, sucrose density gradient sedimentation, and gel electrop

159 ates with monoribosomes and polyribosomes by sucrose density gradient sedimentation.

160 nt outside the lipid rafts, as determined by sucrose density gradient sedimentation.

161 t and beta-mercaptoethanol, and subjected to sucrose density gradient sedimentation.

162 enzyme was constructed and characterized by sucrose-density gradient sedimentation, size-exclusion c

163 Sucrose density gradient separation and immunoblotting w

164 Sucrose density gradients show that EGO is not associate

165 Analysis of soluble hensin by sucrose density gradients showed that low density cells

166 Sedimentation analysis in sucrose density gradients showed that the CSP epitope ac

167 Subfractionation of LDM on sucrose density gradients shows that insulin significant

168 Using sucrose density gradient, size-exclusion chromatography,

169 s of LIV during rate-zonal centrifugation in sucrose density gradients, suggesting that the enzyme is

170 er proteins as its sedimentation behavior in sucrose density gradient suggests an association with th

171 ments (L and R-DNA-gp3) sedimented faster in sucrose density gradients than their proteinase K-treate

172 branes were subfractionated on discontinuous sucrose density gradients to equilibrium or under nonequ

173 study, we used equilibrium sedimentation in sucrose density gradients to separate PrP(Sc) aggregates

174 In sucrose density gradients, Triton X-100-solubilized CaR

175 In sucrose density gradients Trypanosoma brucei NOG1 co-sed

176 Using sucrose density gradient ultracentrifugation and a sensi

177 mmunoprecipitation, confocal microscopy, and sucrose density gradient ultracentrifugation in mice.

178 ligomeric state of the purified complexes by sucrose density gradient ultracentrifugation revealed th

179 Sucrose density gradient ultracentrifugation studies wer

180 We also employed discontinuous sucrose density gradient ultracentrifugation to show tha

181 e approach, called R-DeeP, a method based on sucrose density gradient ultracentrifugation, to identif

182 combination of both confocal microscopy and sucrose density gradient ultracentrifugation, we show th

183 cell culture-grown HCV after purification by sucrose density gradient ultracentrifugation.

184 rification by agarose gel electrophoresis or sucrose density gradient ultracentrifugation.

185 separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation.

186 Carbonate treatment, sonication and sucrose density-gradient ultracentrifugation are subsequ

187 In sucrose density gradients, Vif cosediments with capsid p

188 Using sucrose density gradients we have found that human T cel

189 However, using sucrose density gradients, we demonstrate that the assoc

190 Discontinuous sucrose density gradients were used for fractionation of