ライフサイエンスコーパス: sumoylate

1 several cellular proteins, some of which are sumoylated.

2 pecificity factor and poly(A) polymerase are sumoylated.

3 ucleus, but non-nuclear proteins can also be SUMOylated.

4 Here we show that the RecQ homolog, Rqh1, is sumoylated.

5 pon loading that enhances its capacity to be sumoylated.

6 ht also explain why many COP1 substrates are sumoylated.

7 e investigated the ability of ataxin-1 to be SUMOylated.

8 AR lacking the two lysine residues that are SUMOylated.

9 wever, unlike wild-type cells, mut5 fails to SUMOylate a large set of proteins in response to multipl

10 dentified K68 and K284 as critical sites for SUMOylating actin.

11 re, slx5 cells accumulate phosphorylated and sumoylated adducts of Siz1 in vivo.

12 The proteins SUMOylated after inhibition of ubiquitination were purif

13 at multiple Tup1-interacting proteins become sumoylated after stress.

14 , PHD, Bromo, and HMG domains and are highly sumoylated, all characteristics suggestive of a role in

15 rked increase in PIAS2 expression along with SUMOylated alpha-synuclein in PD brains, providing a cau

16 tide-binding protein Rhes, which is known to sumoylate and disaggregate mtHtt.

17 ro, we found that CPEB3 phase separates when SUMOylated and binds to a specific mRNA target.

18 -like cells resulting in the accumulation of sumoylated and high molecular weight forms of retinoid r

19 We show that all four core histones are sumoylated and identify specific sites of sumoylation in

20 ncreased nuclear abundance, can no longer be SUMOylated and is no longer effective in inhibiting LeEi

21 The sumoylated and nonsumoylated forms of Z were found to ha

22 We found that FOG1 is SUMOylated and phosphorylated in erythroid cells in a di

23 stress through regulation of the turnover of sumoylated and phosphorylated proteins.

24 rgeted Ub ligase display increased levels of sumoylated and polysumoylated proteins, and they are inv

25 ias (WM) using protein macroarrays that were sumoylated and screened for reactivity with paraproteins

26 We found that PML NBs are hyper-SUMOylated and that PML protein is degraded via the ubiq

27 gether, our findings indicate that merlin is sumoylated and that this post-translational modification

28 sidues abolished the ability of S100A4 to be sumoylated and to translocate into the nucleus.

29 Our analysis revealed that H2AX is both sumoylated and ubiquitylated.

30 rement for ICP0-mediated degradation of both sumoylated and unmodified promyelocytic leukemia (PML) a

31 cytic leukemia (PML) nuclear bodies, becomes SUMOylated, and recruits corepressor RIP140 to act as a

32 Three DCC subunits are SUMOylated, and SUMO depletion preferentially reduces th

33 la melanogaster, Mod(mdg4)2.2 and CP190, are sumoylated, and that SUMO is associated with a subset of

34 of several genes implicated in clefting are sumoylated, and the Sumo1 hypomorphic allele interacts g

35 However, NS1's ability to be SUMOylated appears to affect virus multiplication, as in

36 that expressed a RPA70 mutant that cannot be SUMOylated are defective in HR and have a marked increas

37 Conversely, constitutively SUMOylated ARL-13 fully rescues all ciliary defects of a

38 Here, we show that periplakin is SUMOylated at a conserved lysine in its linker domain (K

39 only a small fraction of a target protein is sumoylated at a given time.

40 Here we show that KLF4 is both SUMOylated at a single lysine residue and physically int

41 We determined that p68 and p72 are indeed sumoylated at a single, homologous site.

42 We found that SF-1 is selectively SUMOylated at K194 in Y1 adrenocarcinoma cells and that

43 nerate H4 homogenously and site-specifically sumoylated at Lys-12 (suH4ss).

44 ation results clearly demonstrate that PIP5K SUMOylated at Lys-490 interacts with components of the c

45 We show that lamin A is sumoylated at lysine 201 and that two lamin A mutants as

46 Wor1 is found to be SUMOylated at lysine 385, and loss of this mark by point

47 Finally, we identified that IRF7 is sumoylated at lysine 452.

48 analysis, we demonstrate that TopoIIalpha is SUMOylated at lysine 660 (Lys660), a residue located in

49 Here we show that mouse KLF5 is SUMOylated at lysine residues 151 and 202.

50 nscriptional repressor form of MEF2A that is sumoylated at lysine-403 promoted dendritic claw differe

51 TRalpha was sumoylated at lysines 283 and 389, and TRbeta at lysines

52 Here we show that SERCA2a is SUMOylated at lysines 480 and 585 and that this SUMOylat

53 Here we report that PIF3 is SUMOylated at the Lys13 (K13) residue and that we could

54 Here, we provide evidence that K-bZIP is sumoylated at the lysine 158 residue and associates with

55 KLF2 was sumoylated at two conserved neighboring motifs, but subs

56 Here we show that the corepressor Tup1 is sumoylated, at two specific lysines, under various stres

57 the nucleolar-remodeling complex (NoRC), and SUMOylated BEND3 stabilizes NoRC component TTF-1-interac

58 SUMOylated beta-catenin accumulates at the chromatin and

59 In vivo, SUMOylated beta-catenin and Axin1 are both SENP7S-substr

60 Indeed, Ran is sumoylated both by a reconstituted and the endogenous Ra

61 e SUMO E2 enzyme ubc9 and can be extensively sumoylated both in vitro and in vivo.

62 Here we report that YY1 protein can be sumoylated both in vivo and in vitro.

63 r proteins are likely to be ubiquitinated or sumoylated but not acetylated.

64 epression of the ferritin H ARE; 2) ATF1 was sumoylated, but PIAS3, a SUMO E3 ligase, did not appear

65 Top1 is sumoylated, but Top1 does not appear to be the SUMO subs

66 kinase catalytic subunit and, subsequently, SUMOylated by SUMO E3 ligases protein inhibitors of acti

67 for the sumoylation of Z, and that Z can be sumoylated by SUMO isoforms 1, 2, and 3.

68 , our results reveal that p53 and pRB can be sumoylated by SUMO-2/3 in vivo, and such modification of

69 econd, in vitro-synthesized p32 Pax-6 can be sumoylated by SUMO1, and the sumoylated p32 Pax-6 then c

70 that the K391 and K436 residues of TOP1 are SUMOylated by the PIAS1-SRSF1 E3 ligase complex in the c

71 tment stimulates ULP1a degradation, allowing SUMOylated BZR1 to accumulate and promote growth.

72 Arabidopsis RanGAP1 (AtRanGAP1) lacks the SUMOylated C-terminal domain of vertebrate RanGAP, but c

73 and kinetochores requires interaction of its sumoylated C-terminal domain with the nucleoporin Nup358

74 suggest a model in which Flp that cannot be sumoylated causes DNA damage, whose repair via HRR produ

75 Sumoylated CBS is present in the nucleus where it is ass

76 The global profile of SUMOylated cell proteins was also suppressed by BGLF4 bu

77 ified promyelocytic leukemia (PML) and other sumoylated cellular proteins.

78 ears to be ubiquitin-mediated degradation of sumoylated cellular proteins.

79 at the CDC48A(NPL4) complex actively removes sumoylated CenH3 from centromeres and disrupts centromer

80 alpha-Synuclein disease mutants are more SUMOylated compared with the wild-type protein, and this

81 SUMOylated cSHMT was detected in extracts from S phase M

82 The sumoylated CTE fragment (CTE-SUMO-1) accumulates in the

83 o substantially increases the basal level of SUMOylated Daxx observed in cells.

84 Thus, while pp71 enhances the basal level of SUMOylated Daxx, the role that this modification plays i

85 SUMOylated DCC subunits are enriched at recruitment site

86 gated DELLAs leads to an accumulation of non-SUMOylated DELLAs, resulting in beneficial growth restra

87 onstrated that promoter-bound factors become sumoylated during activation of inducible genes in yeast

88 showed that the yeast activator Gcn4 becomes sumoylated during activation, facilitating its eventual

89 We found that Mre11 and Nbs1 are sumoylated during Ad5 infection and that the E4-ORF3 pro

90 ular that endogenous SEPT7 is constitutively SUMOylated during the cell cycle.

91 s released by murine cerebellar neurons as a sumoylated dynamin-containing protein upon L1 stimulatio

92 F1 and SENP3, which leads to accumulation of sumoylated E2F1.

93 This review will focus on the de-SUMOylating enzymes with special attention to their biol

94 y was not necessary for the SUMOylation, the SUMOylated ErbB4 ICD was tyrosine phosphorylated to a hi

95 of a K-bZIPK158R mutant, which was no longer sumoylated, exhibited the reduced transcriptional repres

96 d that SIRT1 is expressed predominantly as a sumoylated form in cardiomyocyte nuclei.

97 regulation correlates with accumulation of a SUMOylated form of GATA1.

98 ity to small Rho GTPase compared with the un-SUMOylated form of RhoGDI.

99 causes increased steady-state levels of the sumoylated forms of a number of proteins and results in

100 Moreover, SUMOylated forms of mitochondrial proteins particularly

101 g cellular sumoylated species in general and sumoylated forms of PML other than those of PML isoform

102 e recruitment Sp100A(K297R), which cannot be sumoylated, further suggests that sumoylation plays an i

103 Furthermore, we show that clearance of sumoylated Gcn4 requires the protein kinase and Mediator

104 anese patients specifically reacted with the sumoylated heat-shock protein 90 beta isoform-alpha (HSP

105 SUMOylated HP1alpha is enriched at E2F-responsive and me

106 SUMO1-specific paraproteins, suggesting that sumoylated HSP90 promotes pathogenesis of these diseases

107 g deficit was extended to all sites when the sumoylated human mutant (R92Q) protein, which exhibits l

108 The SUMOylated IkappaBalpha form is resistant to signal-indu

109 dent transcriptional repression function for SUMOylated IkappaBalpha.

110 r et al. show that genotoxic stress requires SUMOylated IKKvarepsilon to regulate NF-kappaB transcrip

111 re, we show that ORF29p is ubiquitinated and sumoylated in 293T cells and subsequently degraded from

112 analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 un

113 The ID complex is SUMOylated in a manner that depends on the ATR kinase, t

114 ear the scaffolding domain and that Cav-3 is SUMOylated in a manner that is enhanced by the SUMO E3 l

115 We demonstrate that Topo2a is SUMOylated in an ICRF193-dependent manner by NSE2 at a n

116 moylation, we hypothesized that keratins are sumoylated in an injury-dependent manner and that kerati

117 SOX9 was sumoylated in cotransfection experiments with COS-7 cell

118 DK6 SUMOylation during mitosis; CDK6 remains SUMOylated in G1 phase and drives the cell cycle through

119 tion under resting conditions and is rapidly SUMOylated in response to a kainate but not an N-methyl-

120 tiple proteins of a complex are collectively SUMOylated in response to a specific stimulus, leading t

121 that K102 is the sole CRABP-II residue to be SUMOylated in response to RA.

122 teins FANCI and FANCD2 (the ID complex), are SUMOylated in response to replication fork stalling.

123 We demonstrate that FoxM1b can be SUMOylated in vitro and in vivo, preferentially by SUMO-

124 SOX9 was also sumoylated in vitro by PIAS proteins in the presence of

125 e pancreatic isoform of human glucokinase is SUMOylated in vitro, using recombinant enzymes, and in i

126 18/K19, epidermal keratins, and vimentin are sumoylated in vitro.

127 Using a reconstitution assay, Kar9p was sumoylated in vitro.

128 Rad1's nuclease and Rad10-binding domains is sumoylated in vivo and in vitro.

129 Here, we demonstrate that Mot1 is SUMOylated in vivo and that disrupting the Slx5-Slx8 pat

130 We found that alpha-synuclein is sumoylated in vivo at the same sites in yeast as in huma

131 in vitro, only a small substrate fraction is SUMOylated in vivo, and identification tools for nativel

132 TbAUK1 is SUMOylated in vivo, and mutation of the SUMO-conjugation

133 served Nedd4 family of ubiquitin ligases, is SUMOylated in vivo.

134 fluenza virus and one of the most abundantly SUMOylated influenza virus proteins.

135 egulatory factor 5 (IRF5) as a chaperone for sumoylated IRAK1 nuclear translocation.

136 Our data show that endogenously sumoylated IRF7 is detected in latently infected EBV lym

137 on caused a sharp reduction in the amount of SUMOylated IRF8.

138 e components of a single protein complex are SUMOylated, is also conserved.

139 it Sp1 functions through several mechanisms: sumoylating it at K683 to attenuate DNA binding, and at

140 In the basal state, CPEB3 is SUMOylated; it is soluble and acts as a repressor of tra

141 Following neuronal stimulation, CPEB3 is de-SUMOylated; it now forms oligomers that are converted in

142 Sumoylated KAP1 stimulates the histone methyltransferase

143 showed that the SIM peptide interacted with SUMOylated Ku70 after radiation.

144 ked by silencing RNA directed at the primary SUMOylating ligase, Ubc9.

145 SUMOylated LXRs block NCoR turnover by binding to a cons

146 We identify over a thousand sumoylated lysines in a total of 468 proteins and quanti

147 Although sumoylated MBD1 binds to methylated DNA, it does not inc

148 Collectively, these findings define sumoylated MEF2A and Syt1 as components of a novel cell-

149 sites, as a direct repressed target gene of sumoylated MEF2A in neurons, and demonstrate that repres

150 itic claw differentiation, and expression of sumoylated MEF2A reverses PIASx knockdown-induced loss o

151 MOylation-defective mHTT, Rhes C263S (cannot SUMOylate mHTT), or CRISPR/Cas9-mediated depletion of th

152 its loss causes subunit-specific changes of sumoylated minichromosome maintenance (MCM) helicase in

153 SUMOylated misfolded proteins are then recognized and ub

154 that tumor necrosis factor-alpha (TNF-alpha) SUMOylated MK2 at lysine (K)-339 affected EC actin filam

155 wo target proteins and did not localize bulk sumoylated molecules to nucleoli.

156 Here we show that the Smc5/6 subunit Mms21 sumoylates multiple lysines of the cohesin subunit Scc1.

157 he E2 ligase Ubc9 to FOXM1 generated an auto-SUMOylating mutant (FOXM1-Ubc9).

158 (PR8) NS1 protein, demonstrating that NS1 is SUMOylated not only by SUMO1 but also by SUMO2/3 and map

159 Pias3 SUMOylates Nr2e3, converting it into a potent repressor

160 icase in addition to drastic accumulation of sumoylated nucleolar RENT and inner kinetochore complexe

161 rm of SUMO1) not only increased the level of sumoylated OCT4 (Su-OCT4), but also decreased the stabil

162 Here, we show that Ebp1 p42 isoform can be sumoylated on both K93 and K298 residues, which mediate

163 Here we show that B23 is sumoylated on both Lysine 230 and 263 residues, but the

164 Here, we show that Pin1 is SUMOylated on Lys6 in the WW domain and on Lys63 in the

165 We found OTUB2 to be poly-SUMOylated on lysine 233, and this SUMOylation enables i

166 trated that HIC1 can be either acetylated or SUMOylated on lysine 314.

167 Here we report that merlin can be sumoylated on Lysine residue (K76) in vitro and in vivo.

168 re, we demonstrate that the SnRK1 complex is SUMOylated on multiple subunits and identify SIZ1 as the

169 w that the transcriptional activator Gcn4 is sumoylated on two specific lysine residues and in a mann

170 n motif (SIM)-dependent manner that requires SUMOylated or SUMOylation-competent PML.

171 We show that SoxE mutants that cannot be SUMOylated, or that mimic constitutive SUMOylation, are

172 egion previously shown to associate with and SUMOylate other transcription factors.

173 and TAZ required for their association with SUMOylated OTUB2.

174 32 Pax-6 can be sumoylated by SUMO1, and the sumoylated p32 Pax-6 then can bind to the P3 sequence.

175 helicase (PICH) as an interaction partner of SUMOylated PARP1 in Xenopus egg extract.

176 em mass spectrometry analysis of mitotically SUMOylated PARP1, we identified a residue within the BRC

177 sequence of mitotic SUMOylation, we analyzed SUMOylated PARP1-specific binding proteins.

178 Some NB-associated sumoylated partners also become polyubiquitinated by RNF

179 unctions in unloading of both unmodified and SUMOylated PCNA during DNA replication, while the genome

180 tending D-loops over unextended D-loops when SUMOylated PCNA is present, compared to unmodified PCNA

181 Our results imply that sumoylated PCNA is the physiological ubiquitylation targ

182 We show that Srs2p physically interacts with sumoylated PCNA, which contributes to the recruitment of

183 g parallel quantitation of ubiquitylated and SUMOylated peptides.

184 the C terminus of phyB; the accumulation of SUMOylated phyB is enhanced by red light and displays a

185 how that OVERLY TOLERANT TO SALT 1 (OTS1) de-SUMOylates phyB in vitro, it interacts with phyB in vivo

186 Additionally, SUMOylated PIM1 showed enhanced protein kinase activity

187 s oncogenic signaling through its ability to sumoylate PML and the PML-RARA oncoprotein of acute prom

188 P0 also reduced ICP0-mediated degradation of sumoylated PML in a cooperative manner.

189 UMOylation and noncovalent binding of PML to SUMOylated PML through the SUMO binding motif constitute

190 A prominent ~50-kDa sumoylated protein accumulates in a Ulp1 CC mutant.

191 containing the Huntingtin (HTT) protein and SUMOylated protein corresponds to disease manifestation.

192 esult in dramatic changes in the global host SUMOylated protein profile, but a robust colocalization

193 High molecular weight sumoylated protein species, including PSF, accumulate in

194 ation sites in proteins are hard to predict, SUMOylated protein states are transient in vivo and labi

195 esults suggest that SUMO1, or more likely, a sumoylated protein, acts as an allosteric regulator of D

196 mide-sensitive decrease in a distinct set of SUMOylated proteins (including proteins for chromosome m

197 imide-sensitive increase in a similar set of SUMOylated proteins (including transcription factors and

198 SUMOylated proteins accumulate in rfp1rfp2 double-null c

199 ion for this inviability is that one or more sumoylated proteins accumulate to toxic levels in sgs1De

200 The SUMOylated proteins accumulated predominantly bound to c

201 propose that STUbLs selectively ubiquitinate sumoylated proteins and proteins that contain SUMO-like

202 cleation event for subsequent recruitment of SUMOylated proteins and/or proteins containing SUMO bind

203 nent Red1) and ubiquitin-mediated removal of SUMOylated proteins are also required.

204 vivo, and identification tools for natively SUMOylated proteins are rare.

205 argeted ubiquitin ligases (STUbLs) recognize sumoylated proteins as substrates for ubiquitylation and

206 ion in yeast, we determined the occupancy of sumoylated proteins at a variety of genes by chromatin i

207 Surprisingly, we detected sumoylated proteins at all constitutively transcribed ge

208 s suggests that the CPEB1-ZZ domain recruits sumoylated proteins during assembly of the ribonucleopro

209 Ontology approach to identify differentially sumoylated proteins during heat stress, hyperosmotic str

210 WaLP digestion of SUMOylated proteins generates peptides containing SUMO-r

211 contributes to the detected accumulation of sumoylated proteins in EBV-positive lymphomas.

212 s scarce information on chromatin binding of SUMOylated proteins in HS and the role of chromatin SUMO

213 that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress.

214 The presence of bona fide SUMOylated proteins in the mut5 mutant at 25 degrees C c

215 n of multiple additional subunits of SAGA as sumoylated proteins in vivo, these data suggest that Gcn

216 domain, lead to the accumulation of distinct sumoylated proteins in vivo.

217 However, the identification of endogenous sumoylated proteins is challenging because of the activi

218 The identification of SUMOylated proteins is difficult, because SUMOylation si

219 Unlike ubiquitin, detection of endogenous SUMOylated proteins is limited by the lack of naturally

220 In budding yeast, a subset of sumoylated proteins is targeted for ubiquitination by a

221 discovered a specific set of differentially sumoylated proteins mainly involved in transcription.

222 This discovery suggests that sumoylated proteins may be regulated by ubiquitylation i

223 SENP1, in vitro, indicating the increase in SUMOylated proteins results primarily from inhibition of

224 a ulp2-D623H displayed even higher levels of sumoylated proteins than the corresponding double mutant

225 loss of ULP2 suppresses the toxicity of the sumoylated proteins that accumulate in slx5Delta-slx8Del

226 ligase appears to be needed to ubiquitinate sumoylated proteins that arise in the absence of the Sgs

227 opose a novel function of PICH in remodeling SUMOylated proteins to ensure faithful chromosome segreg

228 ancement enables determination of endogenous SUMOylated proteins under completely native conditions.

229 Substantial increases in SUMOylated proteins upon various stresses have also impl

230 This buildup of SUMOylated proteins was evident within 3-4 h.

231 ng purification under denaturing conditions, SUMOylated proteins were identified by tandem mass spect

232 More than 80% of the SUMOylated proteins whose levels rose or fell upon inhib

233 of whole-cell extracts and nuclear localized SUMOylated proteins with in situ immunofluorescence.

234 Cells lacking Slx8-Rfp accumulate sumoylated proteins, display genomic instability, and ar

235 with cycloheximide prevented the buildup of SUMOylated proteins, they appeared to be newly-synthesiz

236 eported that LMP1 increased global levels of sumoylated proteins, which aided the oncogenic nature of

237 cannot be due simply to high levels of bulk sumoylated proteins.

238 nuclear domains with a high concentration of sumoylated proteins.

239 uantitative proteomics to identify nucleolar SUMOylated proteins.

240 receptors are activated indirectly by other SUMOylated proteins.

241 g the specificity of STUbLs as regulators of sumoylated proteins.

242 which is a ubiquitin E3 ligase that targets sumoylated proteins.

243 targets, contributing to a tight control of SUMOylated proteins.

244 and a concomitant reduction in the levels of SUMOylated proteins.

245 by promoting degradation of other pathogenic sumoylated proteins.

246 metalloprotease Wss1 clears chromatin-bound sumoylated proteins.

247 Upon heat stress, AtBAG7 is sumoylated, proteolytically processed and translocated f

248 t the CAG tract suggests that Slx5/8 targets sumoylated Rad52 for degradation at the pore to facilita

249 SUMOylated RanGAP1 physically interacted with MYCBP2 and

250 A protein mutated such that it cannot be SUMOylated remains localized in the cytoplasm rather tha

251 Mechanistically, sumoylated Rfa1 fostered an interaction with a checkpoin

252 Loss of SMT7 caused elevated SUMOylated RPL30 levels.

253 f RPL30-SUMO4, which mimics elevation of the SUMOylated RPL30 protein in mat3-4, caused a decrease in

254 Our experiments show that SUMOylated Rsp5p has reduced ubiquitin ligase activity,

255 Thus, we demonstrate a novel mechanism for sumoylated S100A4 as a regulator of expression of the MM

256 cells expressing wt PR-B or phospho-mutant (sumoylated) S294A PR-B.

257 Associated Factor B (SAFB) protein, and the SUMOylated SAFB stimulated both the binding of RNA polym

258 rmation and coregulator recruitment of fully sumoylated SF-1 LBD protein was either unchanged or mode

259 teraction of the DEAD-box protein DP103 with sumoylated SF-1.

260 ase 2 (SENP2), significantly reduces nuclear sumoylated SIRT1 levels (P<0.05).

261 lx8, we assayed its Ub ligase activity using sumoylated Siz2 as an in vitro substrate.

262 STUbL recruitment to sumoylated/SLD proteins is mediated by tandem SUMO inter

263 at the SLX4 complex is a SUMO E3 ligase that SUMOylates SLX4 itself and the XPF subunit of the DNA re

264 Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIMDelta) or SUMO-dead alleles (

265 s (SIMs) on Sgs1 that specifically recognize SUMOylated Smc5/6.

266 e absolutely required for targeting cellular sumoylated species in general and sumoylated forms of PM

267 d ubiquitin ligases (STUbLs) can recognize a sumoylated substrate and promote its degradation via ubi

268 a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor Homolog-1 (hL

269 s a SUMO-dependent isopeptidase that acts on sumoylated substrates as they undergo proteasomal degrad

270 ified recombinant human PICH interacted with SUMOylated substrates, indicating that PICH directly int

271 ese modifications by selectively recognizing SUMOylated target proteins through SUMO-interacting moti

272 nonproteolytic, K63-linked ubiquitylation of SUMOylated target proteins.

273 n DNA damage Wss1/Cdc48/Doa1 is recruited to sumoylated targets and catalyzes SUMO chain extension th

274 es during interphase in human cells, but the SUMOylated targets on the chromatin remained unclear.

275 y promote ubiquitin-dependent degradation of SUMOylated targets.

276 We purify SUMOylated TDP1 from mammalian cells and identify the SU

277 ingly, although it was much more efficiently sumoylated than either KLF2 or KLF4, KLF5 was inactive i

278 gating enzyme, physically interacts with and SUMOylates the C terminus of small GTPase ARL-13, the wo

279 lthough a small fraction of Z is known to be sumoylated, the effects of this posttranslational modifi

280 idopsis protoplasts, indicating that ABI5 is sumoylated through SIZ1 and that K391 is the principal s

281 oteins independently of, and separately from SUMOylated TOP2 complexes.

282 vity further promotes TDP2 interactions with SUMOylated TOP2, regulating efficient TDP2 recruitment t

283 ust centromeric Haspin localization requires SUMOylated TOP2A CTD binding activity through SUMO-inter

284 The data indicate that SUMOylated Topo II recruits Aurora B to ectopic sites, c

285 We find that RanBP2 sumoylates Topo IIalpha in mitosis and that this modific

286 ays showed that PICH specifically attenuates SUMOylated TopoIIalpha activity using its SUMO-binding a

287 This PICH function is apparent toward SUMOylated topoisomerase IIalpha (TopoIIalpha) after inh

288 Interestingly, PICH also bound to SUMOylated topoisomerase IIalpha (TopoIIalpha), a major

289 Remarkably, the sumoylated transcriptional repressor form of MEF2A drive

290 1 at the plasma membrane and generation of a sumoylated transmembrane 70-kDa fragment comprising the

291 S could promote the recruitment of PML-IV to SUMOylated TRF1 in TA(+) and ALT cells.

292 and that chromatin-bound Top2p can still be sumoylated, unlike many other SUMO substrates.

293 We found that the kinase PKC-theta was sumoylated upon costimulation with antigen or via the TC

294 We find that all subunits of cohesin become SUMOylated upon exposure to DNA damaging agents or prese

295 nally, we provide evidence that Cos2 is also sumoylated, which counteracts its inhibitory role on Smo

296 Elevated SENP7L renders HP1alpha hypo-SUMOylated, which relieves transcriptional repression of

297 re strongly to the target promoters than its SUMOylated, wild-type counterpart.

298 we have found that the P protein of PIV5 was sumoylated with SUMO1 in both transfected and infected c

299 trate that RNF111 promoted ubiquitylation of SUMOylated XPC (xeroderma pigmentosum C) protein, a cent

300 otein phosphatase activity and ability to be SUMOylated, yet is independent of its lipid phosphatase