ライフサイエンスコーパス: synaptobrevin 2

1 e vesicular SNARE protein VAMP2 (also called synaptobrevin-2).

2 ins of syntaxin1A, SNAP25 (two peptides) and synaptobrevin 2.

3 upon deletion of vSNARE synaptobrevin-1 and synaptobrevin-2.

4 ciated protein-25 (SNAP25), syntaxin-1A, and synaptobrevin-2.

5 ranes that kinetically alters the binding of synaptobrevin-2.

6 vels and localization of SNARE-protein VAMP2/synaptobrevin-2.

7 fected with green fluorescent protein-tagged synaptobrevin 2, a marker of synaptic vesicles.

8 Synaptobrevin-2, a snap receptor (SNARE) protein, partic

9 Syntaxin-1A, SNAP-25, and synaptobrevin-2 (also known as vesicle-associated membra

10 philum through the SNARE proteins Vamp33 and Synaptobrevin 2 and dendritic epidermal T cells.

11 ed that V-ATPase subunit c is localized with synaptobrevin 2 and synaptophysin in synaptic vesicles.

12 s between the synaptic vesicle SNARE protein synaptobrevin 2 and the plasma membrane syntaxin1A/SNAP2

13 naptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasm

14 hen bound to Munc18-1, preventing binding to synaptobrevin-2 and SNAP-25 to form the ternary SNARE co

15 Using recombinant fragments of synaptobrevin-2 and synaptotagmin C2 domains we were abl

16 sealing response was blocked by fragments of synaptobrevin-2 and the C2B domain of synaptotagmin VII.

17 nt on interactions between the vesicle SNARE synaptobrevin-2 and the plasma membrane SNAREs syntaxin-

18 ensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-seri

19 complexes that include the vesicular SNARE, synaptobrevin 2, and that the participation of 5RK in CD

20 contained synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha immunoreactivity, respect

21 Among synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha-IR varicosities, 98% +/-

22 major neuronal vSNAREs, synaptobrevin-1 and synaptobrevin-2, are expressed at the developing neuromu

23 aptic SNARE proteins SNAP-25, syntaxin-1 and synaptobrevin-2, as well as by an age-dependent reductio

24 tion on its substratum, the synaptic protein synaptobrevin 2, attached to the beads.

25 aptic proteins is altered; synaptophysin and synaptobrevin-2 become sequestered in the neuronal soma

26 unilamellar vesicles doped with the v-SNARE synaptobrevin 2 by means of spinning-disc confocal micro

27 hese residues are hydrophilic and constitute synaptobrevin-2 C-terminal flexible region.

28 For example, the binary syntaxin-synaptobrevin 2 complex, in addition to the ternary comp

29 ly due to fewer vesicles of reduced size and synaptobrevin-2 content.

30 nts in astrocytes expressing the fluorescent synaptobrevin 2 derivative, synapto-pHluorin.

31 tics with the results obtained for synthetic synaptobrevin 2-doped vesicles fusing with PSMs of the s

32 BPB also quenched fluorescence of VAMP (synaptobrevin-2)-EGFP, thus indicating the timing of fir

33 absence neuronal vSNARE synaptobrevin-1 and synaptobrevin-2, evoked neurotransmission is completely

34 The residue Y113 of synaptobrevin-2 flexible region was mutated to lysine an

35 omplexin interaction reduces the affinity of synaptobrevin-2 for the 1:1 complex, thereby retarding S

36 ansmembrane SV proteins, synaptotagmin 1 and synaptobrevin 2, from the soma of mature cultured rat an

37 Anti-synaptobrevin 2 immunoprecipitates obtained from freshly

38 units, or synaptophysin, was present in anti-synaptobrevin 2 immunoprecipitates obtained from frozen-

39 SPalpha), synaptophysin, synaptotagmin-1, or synaptobrevin-2 in their axons.

40 Synaptobrevin 2 is thought to facilitate fusion of synap

41 al neurons, but not in neurons obtained from synaptobrevin-2 knockout mice.

42 2 (Delta324-339), block its interaction with synaptobrevin-2 (L348R), or extend the helix to promote

43 lipid-anchored syntaxin-1 and lipid-anchored synaptobrevin-2 lacking TMRs efficiently promoted sponta

44 Our present results show that synaptobrevin-2-like immunoreactivity (-LIR) is present

45 ste cells with synapses display SNAP-25- and synaptobrevin-2-like immunoreactivity (LIR).

46 Synaptobrevin-2-LIR also colocalizes with immunoreactivi

47 Synaptobrevin-2-LIR colocalizes with SNAP-25-, serotonin

48 old immunoelectron microscopy, we found that synaptobrevin-2-LIR is associated with synaptic vesicles

49 However, approximately 27% of the synaptobrevin-2-LIR taste cells do not display IP3R3-LIR

50 rom taste cells onto nerve processes express synaptobrevin-2-LIR, as well as some taste cells without

51 oth type II and type III taste cells display synaptobrevin-2-LIR.

52 All IP3R3-LIR taste cells express synaptobrevin-2-LIR.

53 Thus, synaptobrevin 2 may function in catalyzing fusion reacti

54 s as tetanus toxin did not cleave astrocytic synaptobrevin-2, nor was AA released from pure astrocyte

55 y fluorescent tagging of the vesicle protein synaptobrevin-2 or by staining with the styryl dye FM4-6

56 actor attachment protein receptor) proteins: synaptobrevin 2 (or vesicle-associated membrane protein

57 in FcepsilonRI-regulated exocytosis, whereas synaptobrevin 2- or VAMP-3-deficient mast cells did not.

58 n docking, but little effect on the rates of synaptobrevin-2 proteoliposome fusion.

59 Examining single particle fusion between synaptobrevin-2 proteoliposomes and planar-supported bil

60 Interactions between synaptobrevin 2 (Sb2) and syntaxin 1A (Sx1A) can be read

61 e-associated protein of 25 kDa (SNAP25), and synaptobrevin 2 (Sb2).

62 t the ionic layer by cuffing syntaxin 1A and synaptobrevin 2, similar to the action of SNAP25B; thus

63 s vesicle-associated membrane protein (VAMP)/synaptobrevin 2, SNAP-25, synaptophysin, or synaptotagmi

64 In the absence of synaptobrevin 2, spontaneous synaptic vesicle fusion and

65 Addition of the V(C) peptide synaptobrevin-2 (syb(57-92)) increases the docking effic

66 spectroscopy methods, we find that vesicular synaptobrevin-2 (syb-2) in its monomeric prefusion confo

67 g either synaptobrevin 1 (Syb1(lew/lew) ) or synaptobrevin 2 (Syb2 (-/-)), and those lacking both (Sy

68 on of the SNARE complex by the vesicle SNARE synaptobrevin 2 (syb2) and the two plasma membrane SNARE

69 The neuronal vesicular SNARE protein synaptobrevin 2 (syb2) is anchored in the vesicle membra

70 protein attachment protein receptor (SNARE) Synaptobrevin 2 (Syb2) is known for mediating neurotrans

71 domain (TMD) of the vesicle membrane protein synaptobrevin 2 (syb2).

72 erated four variants of the synaptic v-SNARE synaptobrevin-2 (syb2) anchored to the membrane by lipid

73 cally interacting with vesicle-SNARE protein synaptobrevin-2 (Syb2), which hampers dopamine release.

74 tosomal-associated protein 25 (SNAP-25), and synaptobrevin-2 (Syb2).

75 complex composed of SNAP-25, syntaxin-1, and synaptobrevin-2 (sybII) proteins.

76 In contrast, the apparent number of VAMP2/synaptobrevin 2, synaptophysin, and synaptogyrin demonst

77 rotein receptor (SNARE) complex, composed of synaptobrevin 2, syntaxin and synaptosome-associated pro

78 achment protein receptors complex, including synaptobrevin 2, syntaxin, and synaptosome-associated pr

79 ent protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key ste

80 SNARE complex consists of the three proteins synaptobrevin-2, syntaxin, and synaptosomal-associated p

81 f SNAP-25, and a conserved central domain of synaptobrevin-2, that exhibit a high propensity to form

82 ith the synaptic vesicle SNARE protein VAMP2/synaptobrevin-2, the synaptic vesicle-attached synapsins

83 These observations provide evidence that the synaptobrevin-2 TMD catalyzes the fusion process by its

84 , we show that conformational flexibility of synaptobrevin-2 TMD is essential for efficient Ca(2+)-tr

85 Synaptobrevin-2 transmembrane domain is embedded into th

86 b(G76V), GFP, and a synaptic vesicle protein synaptobrevin-2 (Ub(G76V)-GFP-Syb2); (2) GFP-Syb2; or (3

87 etween two synaptic proteins syntaxin 1A and synaptobrevin 2, using an atomic force microscope and th

88 cholesterol on fusion pore formation between synaptobrevin-2 (VAMP-2)-containing proteoliposomes and

89 We previously reported that a mutation in synaptobrevin 2 (Vamp2) in restless (rlss) mice results

90 Here, we investigated nine synaptobrevin-2 (VAMP2) disease-causing variants and unc

91 lex result in an additional interaction with synaptobrevin-2/VAMP2 (vesicle-associated membrane prote

92 ractions of native alpha-synuclein with both synaptobrevin-2/VAMP2 and anionic lipids.

93 In contrast, the R-SNARE protein synaptobrevin-2/VAMP2 contributes to both regulated and

94 substitution of its SNARE motif with that of synaptobrevin-2/VAMP2 did not.

95 attenuate membrane fusion by competing with synaptobrevin-2/VAMP2 for SNARE-complex assembly.

96 its C-terminal SNARE motif and competes with synaptobrevin-2/VAMP2 for the SNARE-complex assembly.

97 helix 12 in Munc18 within domain 3a leads to synaptobrevin-2/VAMP2 interaction and SNARE complex form

98 that tomosyn's SNARE motif cannot substitute synaptobrevin-2/VAMP2 to form template complexes with Mu

99 te synaptic transmission by cooperating with synaptobrevin-2/VAMP2 to prevent SNAP-25 binding during

100 n complexes composed of syntaxin-1, SNAP-25, synaptobrevin-2/VAMP2, and Munc18-1.

101 Instead, tomosyns extensively bind synaptobrevin-2/VAMP2-containing template complexes and

102 amma-synuclein was not able to interact with synaptobrevin-2/VAMP2.

103 ynuclein directly bound to the SNARE-protein synaptobrevin-2/vesicle-associated membrane protein 2 (V

104 he absence of synaptic vesicle SNARE protein synaptobrevin-2 whereas the increase in spontaneous fusi

105 of 25 kDa (SNAP25), syntaxin-1a (syx-1), and synaptobrevin 2, which is essential for many physiologic

106 unilamellar vesicles containing the v-SNARE synaptobrevin 2, which were docked and fused with lipid-

107 uxtamembranous mutation in the SNARE-protein synaptobrevin-2, which presumably impairs force transfer