ライフサイエンスコーパス: synthetic oligonucleotide

1 strand are adjustable features built into a synthetic oligonucleotide.

2 hanol were synthesized and incorporated into synthetic oligonucleotides.

3 by recursive polymerase chain reaction from synthetic oligonucleotides.

4 design high-density, two-dimensional rays of synthetic oligonucleotides.

5 )-nitrosourea (BCNU) were investigated using synthetic oligonucleotides.

6 amino group on the 5'-terminal phosphate of synthetic oligonucleotides.

7 meter to provide high-throughput analysis of synthetic oligonucleotides.

8 50 bp) were prepared using M13mp19 ssDNA and synthetic oligonucleotides.

9 non-Watson-Crick pairs have been formed with synthetic oligonucleotides.

10 nsity arrays containing tens of thousands of synthetic oligonucleotides.

11 ful for studying the radical's reactivity in synthetic oligonucleotides.

12 y using a minimal fork substrate composed of synthetic oligonucleotides.

13 ycle of co-selection MAGE (CoS-MAGE) with 10 synthetic oligonucleotides.

14 ul for MS/MS-based identity test methods for synthetic oligonucleotides.

15 s are typically assembled from complementary synthetic oligonucleotides.

16 glycosylases when they are incorporated into synthetic oligonucleotides.

17 the characterization of a series of modified synthetic oligonucleotides.

18 or dsDNA analyte including PCR amplicons and synthetic oligonucleotides.

19 s protected phosphoramidite derivatives into synthetic oligonucleotides.

20 as demonstrated using an in vitro assay with synthetic oligonucleotides.

21 ty of origins, including bacteria, mice, and synthetic oligonucleotides.

22 assembly of 5- to 6-kb segments of DNA from synthetic oligonucleotides.

23 emperature (needed for the gene assembly) of synthetic oligonucleotides.

24 Synthetic oligonucleotides (20 bases), representing all

25 Synthetic oligonucleotides (30 and 89 mers) and polymera

26 ingle-stranded calf thymus DNA (ss-ctDNA), a synthetic oligonucleotide, 5'-GATCTATGGACTTACTTCAAGGCCGG

27 se by coupling it to gene repair directed by synthetic oligonucleotides, a process in which a mutant

28 thesizing the open reading frames of L1 from synthetic oligonucleotides, altering 24% of the nucleic

29 s based on the principles of interactions of synthetic oligonucleotide analogues with mRNA.

30 by guided site-specific mutagenesis using a synthetic oligonucleotide and a plasmid carrying an indu

31 cation characteristics were assessed using a synthetic oligonucleotide and DNA extracted from closely

32 Additionally, we demonstrate that both synthetic oligonucleotides and accessible ssDNA targets

33 rotection of methylated restriction sites in synthetic oligonucleotides and can be used to measure me

34 ed product, 5-guanidino-4-nitroimidazole, in synthetic oligonucleotides and DNA treated with peroxyni

35 iodide C-nucleotides were incorporated into synthetic oligonucleotides and exposed to UV-irradiation

36 These genes were assembled by PCR from synthetic oligonucleotides and expressed in Escherichia

37 label (PCB) on the 5'-terminal phosphate of synthetic oligonucleotides and is fully compatible with

38 C element and the proximal Sp1 sites in both synthetic oligonucleotides and natural MAO B core promot

39 Using synthetic oligonucleotides and PCR techniques, each of t

40 ting an artificial transcription bubble from synthetic oligonucleotides and RNA polymerase.

41 VHCDR3 was modified using synthetic oligonucleotides and several clones with reduc

42 bridization of single base mismatch (SBM) in synthetic oligonucleotides and single-nucleotide polymor

43 nces, judged by using fluorophore-conjugated synthetic oligonucleotides and synthetic double-stranded

44 ncoding such proteins were constructed using synthetic oligonucleotides and the protein stability was

45 racts were prepared by the direct cloning of synthetic oligonucleotides, and longer runs were clones

46 We have identified two synthetic oligonucleotides (aptamers) that bind to prost

47 In addition to being important for synthetic oligonucleotides, apurinic site formation in c

48 Unpurified 40-base synthetic oligonucleotides are built into 500- to 800-bp

49 group I introns, ribozymes, RNA aptamers and synthetic oligonucleotides are included.

50 Synthetic oligonucleotides are the main cost factor for

51 Synthetic oligonucleotides are used to regulate gene exp

52 45G variant, X-ray diffraction analysis, and synthetic oligonucleotides as ligands and substrates.

53 of a variety of amine-reactive markers onto synthetic oligonucleotides as well as immobilization on

54 tion and structural diversity of natural and synthetic oligonucleotide assemblies relevant in science

55 Saccharomyces cerevisiae based on annealing synthetic oligonucleotides at the lagging strand of DNA

56 ift assays; SirR retarded the migration of a synthetic oligonucleotide based on the Sir box in a meta

57 RNA aptamers are synthetic oligonucleotide-based affinity molecules that

58 sequence specificity not achievable by using synthetic oligonucleotide-based approaches.

59 screens and the use of both vector-based and synthetic oligonucleotide-based methods for inducing RNA

60 urately, efficiently, and rapidly from short synthetic oligonucleotide building blocks.

61 microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration an

62 erichia coli chromosome from PCR products or synthetic oligonucleotides by homologous recombination.

63 coeluting impurities with similar masses in synthetic oligonucleotides by liquid chromatography-mass

64 3 vector, the vector was modified with short synthetic oligonucleotides by replacing a pelB leader se

65 yme on long RNA substrates, but not on short synthetic oligonucleotides, by enhancing enzyme multimer

66 Immunostimulatory CpG motifs in synthetic oligonucleotides can be effective adjuvants fo

67 ind applications in synthetic biology, where synthetic oligonucleotides can be used to create new too

68 hromatin immunoprecipitation with integrated synthetic oligonucleotides (ChIP-ISO) to systematically

69 a) during reverse transcription, employing a synthetic oligonucleotide consisting of a DNA primer and

70 We have detected DNA binding activity for a synthetic oligonucleotide containing an Sp1 consensus se

71 In stability tests, a synthetic oligonucleotide containing the 5-guanidino-4-n

72 resulting 3'-terminal 49mer fragment with a synthetic oligonucleotide containing the abasic site usi

73 Nuclear extract binding activity to a synthetic oligonucleotide containing the junB CRE-like s

74 enhanced resistance to nuclease digestion of synthetic oligonucleotides containing 4'-thio-2'-deoxynu

75 dium bromide and enhanced by the presence of synthetic oligonucleotides containing binding sites for

76 Synthetic oligonucleotides containing CpG motifs have be

77 Synthetic oligonucleotides containing CpG motifs in spec

78 Here, we show that bacterial DNA, as well as synthetic oligonucleotides containing CpG motifs, induce

79 Bacterial DNA and synthetic oligonucleotides containing CpG sequences (CpG

80 We investigated the adjuvant activity of synthetic oligonucleotides containing CpG-rich motifs li

81 Synthetic oligonucleotides containing different combinat

82 ciency involves XRCC1 or other BER proteins, synthetic oligonucleotides containing either an AP site

83 re we report that repeated administration of synthetic oligonucleotides containing foreign cytosine-p

84 Synthetic oligonucleotides containing FoU at defined sit

85 ng factor, binds tightly and specifically to synthetic oligonucleotides containing G-G base pairs (KD

86 Using synthetic oligonucleotides containing HmU at a defined s

87 res the amount of released 8-oxoguanine from synthetic oligonucleotides containing modified base in t

88 Synthetic oligonucleotides containing the lesion were pr

89 f, and recombinant TRP120 specifically bound synthetic oligonucleotides containing the motif.

90 Synthetic oligonucleotides containing the myb target sit

91 cation experiments showed that 18- and 24-bp synthetic oligonucleotides containing the RepC nick site

92 f the methionine repressor protein, MetJ, to synthetic oligonucleotides containing zero to five speci

93 nucleotides (CpG motifs) in bacterial DNA or synthetic oligonucleotides (CpG DNA) promote the product

94 availability of high-complexity libraries of synthetic oligonucleotides designed to mediate either ge

95 on was supported by gel shift analyses using synthetic oligonucleotide duplexes containing I.C or oth

96 By introducing calibrator synthetic oligonucleotides during library preparation, w

97 Synthetic oligonucleotides, each introducing one amino a

98 By ligation of synthetic oligonucleotides followed by reverse transcrip

99 de cells and thus improve our ability to use synthetic oligonucleotides for controlling cellular proc

100 ences and is retained during cleavage of the synthetic oligonucleotide from the solid support and dur

101 order to enhance DNA triple helix stability synthetic oligonucleotides have been developed that bear

102 NTNFETs with immobilized synthetic oligonucleotides have been shown to specifical

103 Synthetic oligonucleotides have been used to direct base

104 Synthetic oligonucleotides have emerged as effective the

105 Using the technique of randomized synthetic oligonucleotide in cyclic amplification and se

106 oter region can form triplex structures with synthetic oligonucleotides in a sequence-specific manner

107 affinity purification and phosphorylation of synthetic oligonucleotides in conjunction with activated

108 e scale of A-forming tendencies exhibited by synthetic oligonucleotides in solution and the known pol

109 g of endogenous and recombinant nucleolin to synthetic oligonucleotides in which G-rich regions have

110 , it is based on the cloning of 'randomized' synthetic oligonucleotides, in which given position(s) c

111 Although efficient methods exist to assemble synthetic oligonucleotides into genes and genomes, these

112 for nucleic acid-modifying enzymes based on synthetic oligonucleotides labeled with a single fluorop

113 of an alpha-synuclein ep-PCR library and NNS synthetic oligonucleotide libraries are given as example

114 een in adult and pediatric GBM cells using a synthetic oligonucleotide library representing all known

115 on (messenger RNA [mRNA]) using high-density synthetic oligonucleotide microarray analysis (Hu6800 Ge

116 Using synthetic oligonucleotides mimicking the three-strand fl

117 Thus, while bias in randomized synthetic oligonucleotide mixtures can be virtually elim

118 Synthetic oligonucleotides, modeled on the UAS element o

119 repeated DNA sequences generated from single synthetic oligonucleotide monomers are useful for many p

120 Using various synthetic oligonucleotide mutants, GMSAs revealed that o

121 To define the cis-acting element(s), three synthetic oligonucleotides (Na [bp -224 to -195], Nb [bp

122 Synthetic oligonucleotides (ODN) containing TTAGGG motif

123 CpG-containing sequences in bacterial DNA or synthetic oligonucleotides (ODNs) in the endosomal compa

124 Using synthetic oligonucleotides of defined length and sequenc

125 studies of the CD of complexes prepared from synthetic oligonucleotides of varying length suggest tha

126 II) transcription have relied on the use of synthetic oligonucleotides, often artificially discontin

127 Synthetic oligonucleotides (oligos) are important tools

128 he construction of FISH probes entirely from synthetic oligonucleotides (oligos), allowing the optimi

129 etecting large target DNA and a 50-base long synthetic oligonucleotide on the amino-modified microarr

130 Synthetic oligonucleotides (ONs) of different chemistrie

131 data base, miR145, whether transfected as a synthetic oligonucleotide or expressed from a plasmid, c

132 ndomized self-assembly of CRISPR arrays from synthetic oligonucleotide pairs.

133 ences introduced to the electrode surface as synthetic oligonucleotides, PCR products, and RNA transc

134 s capable of direct RTA binding by screening synthetic oligonucleotide pools and KSHV genomic librari

135 ynthesized that enable diene modification of synthetic oligonucleotides prepared by the phosphoramidi

136 as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the

137 Bacterial 16S rDNA was amplified using 2 synthetic oligonucleotide primers specific for eubacteri

138 From a cDNA library and synthetic oligonucleotide probes based on a partial amin

139 l shift and supershift assays with different synthetic oligonucleotide probes demonstrate that protei

140 Based on in situ hybridization studies with synthetic oligonucleotide probes for vasotocin and the r

141 d strand, constructed by ligation of shorter synthetic oligonucleotides, provides layers of the DNA l

142 d was synthesized by overlap extension of 22 synthetic oligonucleotides ranging in length from 70 to

143 Synthetic oligonucleotides rarely contain 100% of the fu

144 The In(2)O(3) surfaces were coupled with synthetic oligonucleotide receptors (aptamers) to recogn

145 Several short synthetic oligonucleotides, rendered resistant to mammal

146 following polymerase binding to a series of synthetic oligonucleotides representing truncations of t

147 econstitution, we found a single molecule of synthetic oligonucleotide (representing the mRNA zipcode

148 detection of low-level impurities present in synthetic oligonucleotide samples with an unprecedented

149 say that measures chromatin accessibility of synthetic oligonucleotide sequence libraries integrated

150 Synthetic oligonucleotide sequences containing Rap1p bin

151 membrane and probed with digoxigenin-labeled synthetic oligonucleotides specific for Actinobacillus a

152 Synthetic oligonucleotide strands ranging from 5 to 25 u

153 of the SuaI endonuclease was determined with synthetic oligonucleotide substrates and modified natura

154 agent on the basis of its ability to cleave synthetic oligonucleotide substrates corresponding to co

155 to psoralen interstrand crosslink-containing synthetic oligonucleotide substrates in vitro.

156 By using a series of synthetic oligonucleotide substrates, we demonstrated th

157 hage N15 and the Klebsiella phage phiKO2 and synthetic oligonucleotide substrates, we directly demons

158 Using synthetic oligonucleotide substrates, we observed that l

159 Synthetic oligonucleotides, such as oligo(dA) and oligo(

160 The assay was validated on synthetic oligonucleotide targets containing the 44 most

161 thymus DNA polymerase delta (pol delta) and synthetic oligonucleotide template-primers containing a

162 The fidelity of pol delta on synthetic oligonucleotide template-primers was compared

163 oliferating cell nuclear antigen (PCNA), and synthetic oligonucleotide template-primers.

164 Antisense oligonucleotides (ASOs) are synthetic oligonucleotides that alter expression of dise

165 Third, we report results on synthetic oligonucleotides that are tagged with a fluore

166 zed as a substrate, a result confirmed using synthetic oligonucleotides that form forked ssDNA/dsDNA

167 midite and have incorporated the analog into synthetic oligonucleotides that have been used in vitro

168 ages, including a reduced number of required synthetic oligonucleotides, the ability to analyze longe

169 ms of immunostimulation by bacterial DNA and synthetic oligonucleotides, the effects of heat shock pr

170 Using a rat C(1)-THF synthase cDNA and synthetic oligonucleotides, the rat C(1)-THF synthase ge

171 th profiling of complex impurity mixtures in synthetic oligonucleotide therapeutics.

172 various adjuvants, including CpG ODN 2006, a synthetic oligonucleotide TLR9 ligand (TLR9L).

173 hymidine dimers and their incorporation into synthetic oligonucleotides to create vinylphosphonate in

174 We have designed synthetic oligonucleotides to give a variety of three-le

175 We used synthetic oligonucleotides to identify bases critical fo

176 we report a modular approach for conjugating synthetic oligonucleotides to proteins with aldehyde tag

177 by recursive polymerase chain reaction from synthetic oligonucleotides to rectify this problem.

178 -2 promoter can form a stable triplex with a synthetic oligonucleotide under pseudo-physiological con

179 geting and invading double-stranded DNA with synthetic oligonucleotides under physiological condition

180 The synthetic oligonucleotides used in these studies contain

181 NA constructs by combining PCR amplicons and synthetic oligonucleotides using golden gate assembly ru

182 nal DNA array which self-assembles from four synthetic oligonucleotides via a four-armed Holliday jun

183 methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the ade

184 Native and modified synthetic oligonucleotides were purified by reversed-pha

185 Synthetic oligonucleotides were used to identify a hexan

186 Synthetic oligonucleotides with a fluorescent coumarin g

187 RecA catalyzes the pairing of synthetic oligonucleotides with one strand of a double-s

188 We have built this lesion into synthetic oligonucleotides, with DHU placed at a single