ライフサイエンスコーパス: synthetic substrate

1 to phosphorylate syntide 2, a Raf-1-specific synthetic substrate.

2 (120 kDa) and to react distinctively with a synthetic substrate.

3 ic defect with prothrombin, but not with the synthetic substrate.

4 noids were accessed chemoenzymatically using synthetic substrates.

5 cine but not phenylalanine residues in model synthetic substrates.

6 ting cleavage of peptidyl-arylamide bonds in synthetic substrates.

7 ulated the amidolytic activity of APC toward synthetic substrates.

8 B while not affecting activity toward small synthetic substrates.

9 specificity and hydrolyzes both natural and synthetic substrates.

10 for mono-ADP-ribosylated arginine residue in synthetic substrates.

11 chemicals for the synthesis of 15 different synthetic substrates.

12 ssessed by enzymatic assay using natural and synthetic substrates.

13 activity in vitro toward comparable specific synthetic substrates.

14 own cleavage sites in proteins, peptides and synthetic substrates.

15 id substitutions within the P5-P4' region of synthetic substrates.

16 achieved with high concentrations of short, synthetic substrates.

17 y and catalytic potential using a variety of synthetic substrates.

18 P2 exhibited lipase activity against several synthetic substrates.

19 egioselective macrocyclization of natural or synthetic substrates.

20 ssentially inactive toward the commonly used synthetic substrate 1-chloro-2,4-dinitrobenzene (CDNB),

21 his ostensibly dead general base mutant by a synthetic substrate, 3beta-hydroperoxycholestane (3HPC)

22 trate 4-hydroxy-2-ketoheptane-1,7-dioate and synthetic substrates 4-hydroxy-2-ketopentanoic acid and

23 domain of alpha-mannosidase IIx hydrolyzes a synthetic substrate, 4-umbelliferyl-alpha-D-mannoside, a

24 an fibronectin, and the low-molecular-weight synthetic substrate 7-amino-4-trifluoromethyl coumarin.

25 es from its unusual specificity for cleaving synthetic substrates after aspartate residues.

26 analyses of 20S proteasomes with natural or synthetic substrates allowed to infer the substrate spec

27 d is distinct from the mechanism utilized by synthetic substrate analog inhibitors.

28 Inhibitor screening with synthetic substrate analogs revealed that PgpB is inhibi

29 Assays performed on 20 synthetic substrate analogs revealed the enzyme specific

30 R domains and analyzed their reactivity with synthetic substrate analogs.

31 The use of a designed, synthetic substrate analogue allows unique insights into

32 microm), d-kynurenine (Ki = 12 microm) and a synthetic substrate analogue D,L-3,7-dihydroxydesaminoky

33 Using synthetic substrate analogues and products containing di

34 Using synthetic substrate analogues, it is shown that AbyA5 ca

35 tion of Cdc25A activity in vitro, using both synthetic substrates and authentic cellular substrate, i

36 Here, using synthetic substrates and biochemical assays, we show tha

37 as measured both by an enzymatic assay with synthetic substrates and by detection of proteolytically

38 d by up to 10-fold the BrAAP activity toward synthetic substrates and by more than 2-fold the degrada

39 ressed human GALNS indicate activity against synthetic substrates and inhibition by both substrate an

40 Traditional synthetic substrates and matrices for cell culture have

41 e is being depleted, overcoming the need for synthetic substrates and reducing postreaction processin

42 te specificity as MIHCK when assayed against synthetic substrates and that PAK1 phosphorylates the he

43 -dependent MMP with unique specificity among synthetic substrates and the capability to both degrade

44 re challenging to identify their natural and synthetic substrates and to distinguish their localizati

45 tabolized gamma-carboxyl-linked glutamate of synthetic substrates and tubulin.

46 regation, thrombin esterolytic activity on a synthetic substrate, and thrombin cleavage of fibrinogen

47 rd factor B, low esterolytic activity toward synthetic substrates, and absence of regulation by zymog

48 osynthesis were characterized in vitro using synthetic substrates, and activity was measured by LC-MS

49 he reaction is analyzed based on a series of synthetic substrates, and control experiments and DFT ca

50 Proenzyme MASP-1 R448Q readily cleaves synthetic substrates, and it is inhibited by a specific

51 he regioselective cyclization of a surrogate synthetic substrate, Ant-l-Kyn-N-acetylcysteamine, to gi

52 e with comparable specific activities toward synthetic substrates as reported for cathepsin L.

53 d PC3/FurPd had elevated activity on several synthetic substrates as well as reduced calcium ion depe

54 ase catalyzes the conversion of oQ to Q in a synthetic substrate, as well as undermodified RNA isolat

55 By use of synthetic substrate assays, dog plasma was shown to cont

56 heat stable and has optimal activity on the synthetic substrate at or above pH 8.0.

57 zed cleavages occurred at a single site in a synthetic substrate based on the prolactin (Prl) signal

58 ions did not significantly affect Km for the synthetic substrate but decreased the kcat and increased

59 tic activity of FXa toward cleavage of small synthetic substrates, but the role of Na(+) in the proth

60 eration of the proteolytic hydrolysis of the synthetic substrate C1-1 by C1s, enhancement of the comp

61 The hydrolysis of the synthetic substrate C1-1 catalyzed by C1s in 25 to 27 mi

62 These synthetic substrates can also be used as sensitive probe

63 efficiency of glycosylation reactions using synthetic substrates carrying the same glycan structure

64 and Ca2+ with increasing concentrations of a synthetic substrate (CH3-SO2-d-Leu-Gly-Arg-p-nitroanilid

65 There is no previous report of synthetic substrate cleavage by fI, but five substrates

66 the aptamer does not completely block small synthetic substrate cleavage, although it does slow the

67 of binding to four-way junction DNA (4H), a synthetic substrate commonly used to study proteins invo

68 novel in situ zymography method that uses a synthetic substrate conjugated to a fluorescent tag for

69 rface of microorganisms and incubated with a synthetic substrate consisting of (1) a fluorescent dye

70 The assay uses a synthetic substrate containing three chemical moieties:

71 binant forms of TACE efficiently processed a synthetic substrate corresponding to the TNF alpha cleav

72 Avr-Rpt2-dependent proteolytic cleavage of a synthetic substrate, demonstrating that these sequences

73 like activity as revealed by cleavage of the synthetic substrate, DEVD-pNa, by cell cytosols, and als

74 c lipase by a maximum of 72.2% (+/-4.1) with synthetic substrate (DGGR) and 58.0% (+/-9.7) with natur

75 kinetics for exon 5 chimera cleavage of two synthetic substrates display an MMP-3 phenotype, however

76 ss hydrolysis of 3[H]-benzoyl-Phe-Ala-Pro, a synthetic substrate for angiotensin converting enzyme, a

77 tivated HaCA42 carboxypeptidase hydrolysed a synthetic substrate for glutamate carboxypeptidases (FAE

78 transition state, we synthesized a series of synthetic substrates for DNA C5-MTase containing DZCyt.

79 amework for the combinatorial development of synthetic substrates for stem cell culture.

80 ults additionally should allow the design of synthetic substrates for use in biochemical purification

81 of polyketide synthases (PKSs) has relied on synthetic substrates functionalized as electrophilic est

82 specificity and activity against gelatin and synthetic substrates, further supporting the notion that

83 erence in trypsinogen recognition, the small synthetic substrate Gly-Asp-Asp-Asp-Asp-Lys-beta-naphthy

84 The activity was assayed with a synthetic substrate hydroxyethyl disulfide.

85 The assay uses a newly designed synthetic substrate (IdS-S) consisting of alpha-L-iduron

86 The activity of caspase 3 to cleave the synthetic substrate in vitro and PARP in vivo is blocked

87 c activity of caspase 3 to cleave a specific synthetic substrate in vitro is detected in L. pneumophi

88 DPRT) domain with minimal activity towards a synthetic substrate in vitro.

89 iferase can discriminate between natural and synthetic substrates in the brains of live mice.

90 cells in culture and recombinant enzymes and synthetic substrates in vitro have pointed to the subtil

91 r439, enhanced enzymatic activity of Xrn2 on synthetic substrates in vitro.

92 In vitro assays with appropriate synthetic substrates indicate that CE can promote RLF in

93 bstrate C3bB, but not proteolysis of a small synthetic substrate, indicating that AFD did not block a

94 ction to initiate unwinding and resolves the synthetic substrate into two double-stranded fork struct

95 s transgenic plants indicate that the AtAtg8 synthetic substrate is efficiently processed and this is

96 ive site, since amidolytic activity toward a synthetic substrate is largely unaffected.

97 Catalytic efficiency toward synthetic substrate is lower, but it has a broad specifi

98 Unwinding by UL9 of a synthetic substrate is significantly inhibited by a bis-

99 perbolic mixed modifier in the presence of a synthetic substrate, it completely inhibits collagen deg

100 from a range of substrates, including small synthetic substrates, larger peptides, and proteins.

101 By using the combinatorial methods of synthetic substrate libraries and substrate-phage displa

102 Using the synthetic substrates Lys-Pro-Gln-pNA and Gly-Gly-Gln-pNA

103 umor necrosis factor-alpha converting enzyme synthetic substrate, Mca-PLAQAV-Dpa-RSSSR-NH(2).

104 activity, with or without FVIIIa, toward the synthetic substrate, methylsulfonyl-D-cyclohexylglycyl-a

105 A synthetic substrate mimetic inhibited diverse fungal Cdc

106 Using a synthetic substrate (MUNANA), all neuraminidases showed

107 te acceleration toward the hydrolysis of the synthetic substrate N-benzoyl-arginine-p-nitroanilide wh

108 Km was 4.28+/-0.34mM of the synthetic substrate N-benzoyl-dl-arginyl-rho-nitroanilid

109 y processed enzyme was able to hydrolyze the synthetic substrate N-t-butyloxycarbonyl-L-alanyl-L-alan

110 It hydrolyzed the synthetic substrates N-succinyl-Ala-Ala-Pro-Phe p-nitroa

111 In vitro testing of synthetic substrates of S. mansoni sirtuin 2 (SmSirt2) a

112 state of vimentin by the inclusion of small synthetic substrates of the enzyme dansylcadaverine or d

113 ombinant phosphatase is active in vitro on a synthetic substrate or on isolated thylakoids.

114 Thus, binding of small synthetic substrates or inhibitors provides sufficient e

115 in exhibits phosphatase activity against the synthetic substrate p-nitrophenyl phosphate.

116 displayed similar activities toward a small synthetic substrate, p-nitrophenyl phenylphosphonate, su

117 -Glucosidase activity was measured using the synthetic substrate, p-nitrophenyl-beta-D-glucoside.

118 n of chymotrypsin and renin activities using synthetic substrates P4 (F-R-R-R-F-V-R-R-F-NH2) and P5 (

119 The transfer of 33PO4 from ATP to the synthetic substrate poly(Glu, Tyr) 4:1 attached to the b

120 hia coli-expressed Lpro unable to cleave the synthetic substrate pro-ISG15 while preserving cellular

121 This synthetic substrate produced slow decay glow kinetics th

122 ream caspases as determined by cleavage of a synthetic substrate, proteolysis of poly(ADP-ribose) pol

123 Investigations using synthetic substrates reveal that GlfT2 is processive.

124 The converted active plasmin cleaved the synthetic substrate S-2251, and the natural substrates f

125 itive to thrombin, MzII(a), and Pre2-F1.2; a synthetic substrate (S-2238) detected thrombin or MzII(a

126 ular interactions between FXIa and the small synthetic substrate (S-2366), the macromolecular substra

127 plasmin was generated as detected by plasmin synthetic substrate (S2403) hydrolysis; however, after t

128 ited not only enzyme isoform selectivity but synthetic substrate selectivity as well.

129 Inhibitor and synthetic substrate studies further suggest that activat

130 Pro) II helical peptides represent the first synthetic substrates successfully designed for aggrecana

131 ystems, wherein a live cell interacts with a synthetic substrate supported membrane, allow interrogat

132 d fluorescence microscopy indicated that the synthetic substrates supported detectable binding of a c

133 to 3-methylindolic acid linked to Cys8 of a synthetic substrate surrogate as well as the formation o

134 of an engineered module was achieved using a synthetic substrate surrogate that serves as a Michael a

135 viscosity on catalysis were determined with synthetic substrates that bind to all of the enzymic sub

136 is problem, we have developed an assay using synthetic substrates that can discriminate between the d

137 Our results indicate that synthetic substrates that recognize cell-surface glycans

138 Using synthetic substrates that span kPa-GPa moduli, we found

139 a "turn-on" reporter strategy, which employs synthetic substrates that yield a fluorescent product up

140 In addition to synthetic substrates, the neuropeptides, vasoactive inte

141 n tested against a wide range of natural and synthetic substrates, this enzyme showed a preference fo

142 cence resonance energy transfer-based AtAtg8 synthetic substrate to assess AtAtg4 activity in vitro a

143 t chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of f

144 an IgG anti-tryptase mAb, on cleavage of the synthetic substrate tosyl-Gly-Pro-Lys-p-nitroanilide and

145 GSH itself, oxidized GSH, and the synthetic substrates used to analyze GGT activity (gamma

146 A synthetic substrate was co-crystallized with a catalytic

147 In contrast, the activity of SHP-1 against a synthetic substrate was not affected by the mode of plat

148 ty of the 600-kDa alpha(1)beta-proteasome on synthetic substrates was cleavage carboxyl to hydrophobi

149 that detects ricin mediated depurination of synthetic substrates was improved through optimization o

150 osides, while degradation of the beta-linked synthetic substrates was restricted to strains within th

151 Using these synthetic substrates we reveal that the SUMO domain enha

152 Using biotin-based synthetic substrates, we established that the three enzy

153 Using a panel of synthetic substrates, we have systematically investigate

154 MmpE_ACP-bound synthetic substrates were critical in demonstrating succ

155 Various synthetic substrates were efficiently cleaved with a str

156 y Na+ increased the affinity of FIXa for the synthetic substrate, whereas occupancy by Ca2+ decreased

157 Studies on a synthetic substrate, which has an anomeric carbon atom w

158 nic Arabidopsis plants expressing the AtAtg8 synthetic substrate will be a valuable tool to dissect a

159 aled that phosphoparacetamol is an excellent synthetic substrate with a Km value of 1.2 mM.

160 uced k(cat) and similar K(m), and hydrolyzed synthetic substrate with approximately 10-fold reduced K

161 Using a synthetic substrate with these characteristics, we enric

162 Recombinant Nna1 metabolizes synthetic substrates with 2 or more C-terminal glutamate

163 psin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.

164 te that matriptase is able to cleave various synthetic substrates with arginine or lysine as their P1

165 Various synthetic substrates with either Phe or Val in the P1 po

166 Synthetic substrates with fluorescent leaving groups exh

167 Thus, synthetic substrates with specific 3-D microgeometries c

168 on of peptidase to a solution containing the synthetic substrate yields a change in electrode EMF res

169 our assay as it did in the hydrolysis of the synthetic substrate Z-Phe-Leu-Glu-pNA (kcat/Km value of