ライフサイエンスコーパス: tRNA(Ser)

1 mcd2-1, and show that the mutation lies in a tRNA(Ser)(CGA), which has been modified to translate the

2 We demonstrate that a tRNA(Ser(UCN)) precursor with the U7445C substitution ca

3 38 in the anticodon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Ser)UGA, and selenocysteine tRNA with

4 extent, differences in the in vivo tRNA(Ala):tRNA(Ser) ratio in 159 and Pn16.

5 This minimally altered tRNA(Ser) exclusively inserted leucine residues and was

6 His(GUG)) for Um, and tRNA(Pro(GGG)) for Am. tRNA(Ser(UGA)), previously observed as a TrmJ substrate

7 ptional regulation, inositol metabolism, and tRNA(Ser)(CGA) abundance.

8 Although SerRS recognizes both tRNA(Sec) and tRNA(Ser) species, PSTK must discriminate Ser-tRNA(Sec)

9 condary structures of archaeal tRNA(Sec) and tRNA(Ser), we introduced mutations into Methanococcus ma

10 a unique location between the tRNA(His) and tRNA(Ser (AGY)) genes.

11 ends) analyses indicated that the four-armed tRNASer(UCN) gene is transcribed into a stable RNA that

12 cates that similarity between the four-armed tRNASer(UCN) genes is only 63.8% compared with an averag

13 he M. californianus and M. edulis four-armed tRNASer(UCN) sequences are interpreted as pseudo-tRNASer

14 ylation of variant transcripts of M. barkeri tRNASer was kinetically analyzed in vitro with pure enzy

15 ntity elements into the mitochondrial bovine tRNA(Ser) scaffold yielded chimeric tRNAs active both in

16 codon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Ser)UGA, and selenocysteine tRNA with UCA (tRNA([Se

17 The HNH nuclease cleaves tRNA(Ser), stalling protein synthesis and arresting vira

18 1--72 through 5--68 base pairs of the E.coli tRNA(Ser) acceptor stem with the major recognition eleme

19 precision values for the analyses of E. coli tRNASer(VGA) and E. coli tRNAThr(GGU), unfractionated tR

20 Dihydrouridine content of Escherichia coli tRNASer(VGA) and tRNAThr(GGU) as controls were measured

21 d by the DHU arm-replacement loop-containing tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and t

22 ation of unspliced precursor RNAs of dimeric tRNA(Ser)-tRNA(Met)i, suggesting a novel nuclear role fo

23 nt of 28 isolated mutants contain duplicated tRNA(Ser)UCA-C47:6U genes.

24 four- to fivefold excess over the endogenous tRNA(Ser).

25 cations, we used a genetic screen to examine tRNA(Ser(CGA)) variants.

26 DNA sequence analysis of cDNA clones for tRNA(Ser) and 18S rRNA confirmed the expected 3'-termina

27 f most tRNA but with a marked preference for tRNA(Ser), to which long stretches of cytidines are adde

28 tigate the requirements of these enzymes for tRNASer recognition, serylation of variant transcripts o

29 eotides at the base of the variable stem for tRNASer recognition, unlike its bacterial type counterpa

30 NA(Sec) were crucial for discrimination from tRNA(Ser).

31 jannaschii PSTK distinguishes tRNA(Sec) from tRNA(Ser).

32 ich structural or sequence elements of human tRNA(Ser) are necessary for pseudouridine (Psi) formatio

33 y, carrot protoplasts transfected with human tRNA(Ser)AUC genes containing the lac operator (lacO) in

34 MenT3 targets in M. tuberculosis identifies tRNA(Ser) as the sole target of MenT3 and reveals signif

35 o acid identity and recognition of a type II tRNA(Ser) amber suppressor from a serine to a leucine re

36 Furthermore, the A5-U68 base pair in tRNA(Ser) has some antideterminant properties for PSTK.

37 ; the G15-C48 tertiary "Levitt base-pair" in tRNA(Ser) was changed to A15-U48; the number of nucleoti

38 ed to a disproportionately large increase in tRNA(Ser)UCA-C47:6U levels in sla1-rrm but not sla1-null

39 uch a distinction between the two enzymes in tRNASer identity determinants reflects their evolutionar

40 Among the tRNA(Ser) isoacceptors, tRNA(Ser(GCU)) decreased the most in ELAC2-deficient cel

41 We discuss how the existence of a large tRNA(Ser) gene family may permit this suppression withou

42 mutants lacking m(7)G and m(5)C, and mature tRNA(Ser(CGA)) in mutants lacking Um and ac(4)C.

43 Nase P, was found to process a mitochondrial tRNA(Ser(UCN)) precursor [ptRNA(Ser(UCN))] at the correc

44 ion of the deafness-associated mitochondrial tRNA(Ser(UCN)) T7511C mutation, in conjunction with homo

45 addition to the non-canonical mitochondrial tRNA(Ser(AGY)), but no obvious qualitative differences i

46 utations investigated in human mitochondrial tRNA(Ser(UCN)) affect processing efficiency, and some af

47 Mammalian mitochondrial tRNA(Ser(UCN)) (mt-tRNA(Ser)) and pyrrolysine tRNA (tRNA

48 n patient fibroblasts rendered mitochondrial tRNA(Ser(AGY)) undetectable, and markedly reduced mitoch

49 from defective CCA addition to mitochondrial tRNA(Ser(AGY)), and that the severity of this biochemica

50 associated with less accurate mitochondrial tRNASer(AGY) processing from the primary transcript and

51 s) gene at its 5' end and by 23 bp of the mt tRNA(Ser (AGY)) gene at its 3' end.

52 ([Ser]Sec)UCA level was increased and the mt tRNA(Ser)UGA level was decreased, suggesting that TRIT1

53 While mt-EF-Tu2 is specific for D-armless mt-tRNA(Ser), mt-EF-Tu1 recognizes the remaining 20 tRNAs.

54 Furthermore, in human mt-tRNA(Ser), lengthening the variable loop by the 7472insC

55 ts confirm that insertion mutations lower mt-tRNA(Ser) melting temperature by 6-9 degrees C and incre

56 traints and hence destabilizes the mutant mt-tRNA(Ser) by approximately 0.6 kcal/mol relative to wild

57 ble loop by the 7472insC mutation reduces mt-tRNA(Ser) concentration in vivo through poorly understoo

58 e unique secondary structure of wild-type mt-tRNA(Ser) decrease the entropic cost of folding by appro

59 Mammalian mitochondrial tRNA(Ser(UCN)) (mt-tRNA(Ser)) and pyrrolysine tRNA (tRNA(Pyl)) fold to near

60 The well-balanced translation of mt-tRNA(Ser(UCN))- and mt-tRNA(Thr)-dependent codons throug

61 filing uncovered mitoribosome stalling on mt-tRNA(Ser(UCN))- and mt-tRNA(Thr)-dependent codons.

62 3)C) methylation at position C(32) of the mt-tRNA(Ser(UCN)) and mt-tRNA(Thr).

63 duplication and loss of function, and a new tRNA(Ser(UCN)) gene has been created de novo.

64 in addition the G73 "discriminator" base of tRNA(Ser) was changed to A73.

65 The substrate, composed of tRNA(Ser) and tRNA(Leu), is transcribed in tandem with a

66 t in complex with the anticodon stem loop of tRNA(Ser) bound to the PsiAG stop codon in the A site.

67 such as the purine-pyrimidine base pairs of tRNA(Ser).

68 Some point mutations in the ASL stem of tRNA(Ser) had significant effects on the levels of modif

69 -loop are seen in the cocrystal structure of tRNA(Ser) and Thermus thermophilus seryl-tRNA synthetase

70 directly following the discriminator base of tRNA(Ser(UCN))) causes non-syndromic deafness.

71 in response to Ser limitation, regulation of tRNA(Ser(GCU)) levels fine-tune the mTE of UC[C/U] or AG

72 mutation that disrupts the anticodon stem of tRNA(Ser)CGA require Lhp1p for growth.

73 ta show a sharp threshold in the capacity of tRNASer(UCN) to support the wild-type protein synthesis

74 he prevailing understanding that deletion of tRNASer(UGA) (serT) would render the serine codon compre

75 21 derived from four isoacceptor families of tRNASer genes, 7 from five families of tRNALeu genes, an

76 two SerRSs do not possess a uniform mode of tRNASer recognition, and additional determinants are nec

77 quence A36A37A38 in the anticodon loop, only tRNA(Ser)AGA, tRNA(Ser)CGA, tRNA(Ser)UGA, and selenocyst

78 Although maturation of the mutant pre-tRNA(Ser)CGA requires Lhp1p, introduction of a second mu

79 Suppressor pre-tRNA(Ser)UCA-C47:6U with a debilitating substitution in

80 We demonstrate that for the wild-type pre-tRNA(Ser)CGA and other pre-tRNAs, Lhp1p is required for

81 Ser(UCN) sequences are interpreted as pseudo-tRNASer(UCN) genes.

82 o distinguish tRNA(Sec) from closely related tRNA(Ser) substrate.

83 ucture, while two [tRNASer(AGN) and a second tRNASer(UCN)] will fold only into tRNAs with a dihydrour

84 STK must discriminate Ser-tRNA(Sec) from Ser-tRNA(Ser).

85 reover, a novel determinant for the specific tRNASer recognition was identified as the anticodon stem

86 ion to the point where the ochre-suppressing tRNA(Ser) is in four- to fivefold excess over the endoge

87 ppression by the weak ochre (UAA) suppressor tRNA(Ser) SUQ5.

88 an active pool of endogenous MenT3 targeting tRNA(Ser) in M. tuberculosis is detected, likely reflect

89 Here, we report evidence that tRNASer(CGA) (serU) can, surprisingly, also decode TCA,

90 Among the tRNA(Ser) isoacceptors, tRNA(Ser(GCU)) decreased the mos

91 antation of these identity elements into the tRNA(Ser)(UGA) scaffold resulted in phosphorylation of t

92 Deletion analysis showed that the tRNA(Ser) TPsiC stem-loop was a determinant for modifica

93 (SerDelta); an amber suppressor in which the tRNA(Ser) type II extra-stem-loop is replaced by a conse

94 However, in the eremobatid, the tRNA(Ser(UCN)) gene in the repeat region appears to have

95 This amount of reduction in the tRNA(Ser(UCN)) level is below a proposed threshold to su

96 exhibited approximately 75% decrease in the tRNA(Ser(UCN)) level, compared with three control cybrid

97 when lacO was located at position -1 of the tRNA(Ser)AUC coding sequence.

98 sequence analyses found a duplication of the tRNA(Ser)UCA-C47:6U gene, which was shown to cause the p

99 verage reduction of approximately 70% in the tRNASer(UCN) level and a decrease of approximately 45% i

100 e that the mutation flanks the 3' end of the tRNASer(UCN) gene sequence and affects the rate but not

101 7 kbp upstream and is cotranscribed with the tRNASer(UCN) gene, with strong evidence pointing to a me

102 cture of the homologous Thermus thermophilus tRNA(Ser)-SerRS complex that Cusack and colleagues repor

103 -encoded tRNAs with A36A37A38, only mt tRNAs tRNA(Ser)UGA and tRNA(Trp)UCA contained detectable i6A37

104 r-armed tRNA secondary structure, while two [tRNASer(AGN) and a second tRNASer(UCN)] will fold only i

105 ins 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes.

106 rm-replacement loop-containing tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and tRNAPro genes o

107 Unexpectedly, tRNA(Ser(GCU)) delivery restored AG[U/C] mTE and reduced

108 eit at a lower level than tRNA(Sec), whereas tRNA(Ser) did not.

109 ic tRNAs are charged at >80% levels, whereas tRNASer and tRNAThr are charged at lower levels.

110 Moreover, while tRNA(Ser) levels were unaffected by TRIT1 knockdown, the