ライフサイエンスコーパス: tagging

1 ns produced in the decay of (136)Xe ("barium tagging").

2 e intensity of the moving targets (frequency tagging).

3 ich are well-suited for surface glycoprotein tagging.

4 tor, suggesting a role for Abeta in synaptic tagging.

5 gy-mediated end joining repair for efficient tagging.

6 strate precise, scalable, and efficient gene tagging.

7 equence, resulting in easy and flexible gene tagging.

8 lation of the labeled transcripts used in TU tagging.

9 solution localization microscopy and protein tagging.

10 pse-specific modification, known as synaptic tagging.

11 agenesis and green fluorescent protein (GFP) tagging.

12 and presence or absence of contrast material tagging.

13 proteins and their recalcitrance to epitope tagging.

14 proteins were marked by fluorescent protein-tagging.

15 hy phantom, especially with suboptimal fecal tagging.

16 by photo-cross-linking and subsequent biotin-tagging.

17 Genetic tagging (19 microsatellites) revealed 18% of re-sampled

18 th 91% crossing receiver lines one year post tagging, 61% detected after year two at large, with dete

19 are required to achieve a 90% possibility of tagging a given gene in the B. distachyon genome using t

20 Amino acid-coded mass tagging (AACT) with stable isotopes followed by tandem m

21 time-to-digital conversions with photon time-tagging accuracy limited to approximately ns.

22 Existing approaches based on tagging acquisitions may introduce artifacts in MR image

23 ocalisation of Organelle Proteins by Isotope Tagging after Differential ultraCentrifugation (LOPIT-DC

24 ion, we used Cre recombinase-dependent miRNA tagging and affinity purification in mice.

25 ration (r = 0.84), while correlation between tagging and blood myocardial border tracing (r = 0.36) a

26 ative plasticity mechanisms such as synaptic tagging and capture (STC) were impaired already in presy

27 how associative memory mechanisms, synaptic tagging and capture (STC), was impaired in SD mice at ce

28 Here, we show that SD impaired synaptic tagging and capture and behavioral tagging, two major me

29 observed experimentally, including synaptic tagging and capture phenomena.

30 ults underscore the utility of inducible RNA tagging and challenge current models of telomerase matur

31 s thought to involve the ubiquitin-dependent tagging and degradation through endo-lysosomal and autop

32 oding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry.

33 agging sequencing (PhOTseq), a technique for tagging and expression profiling of cells on the basis o

34 ferential strain (PSCS) was calculated using tagging and feature-tracking software with different alg

35 ryo-electron tomography coupled with epitope tagging and gold labeling reveal that FAP57 forms an ext

36 n NOM is based on a combination of molecular tagging and high resolution spectroscopic techniques, su

37 Tagging and interaction proteomics of 12 identified prot

38 Finally, synaptic tagging and long-term memory deficits in mice lacking tr

39 imity labeling can be combined with isobaric tagging and mass spectrometry to enable quantitative, ti

40 Isobaric tagging and metabolic labeling, namely, tandem mass tagg

41 from the traditional bioinformatics use for tagging and navigation and has necessitated the developm

42 ent revealed the highest correlation between tagging and non-rigid, elastic image registration (r = 0

43 Both mRNA pyrimidine tagging and re-adenylation are dependent on the same ter

44 Here, we asked whether tagging and stimulating cells along the dorsoventral axi

45 Using long-term electronic tagging and survey data we reveal rare and cryptic inter

46 normal breeding success in both the year of tagging and the following year, incubating birds carryin

47 ntify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneousl

48 tabolic RNA labeling method (thiouracil (TU) tagging) and a pipeline to detect the labeled transcript

49 atory procedures including fin clipping, PIT tagging, and nociceptor excitation via injection of acet

50 uld potentially be used for shot-to-shot CEP tagging applications requiring orders-of-magnitude less

51 odifying precursor emissions) as well as the tagging approach (i.e., tracking ozone produced from spe

52 ibosome profiling (Ribo-seq) with a ribosome-tagging approach (Ribo-tag), it was possible to determin

53 We show that the mdDiLeu tagging approach for multiplexed DIA is a viable methodo

54 ) and the products were detected in a double-tagging approach using a biotinylated capture probe for

55 rends that emerged from this systematic gene-tagging approach.

56 ploying mass difference labeling or isobaric tagging are incompatible with DIA.

57 n of sequence-specific mutations and epitope tagging at an endogenous locus.

58 -based biorthogonal non-canonical amino acid tagging (BONCAT) - for studying the activity and biogeoc

59 and bio-orthogonal non-canonical amino acid tagging (BONCAT) followed by SWATH quantitative mass spe

60 SILAC)/bioorthogonal noncanonical amino acid tagging (BONCAT) mass spectrometry (MS).

61 adapt bioorthogonal noncanonical amino acid tagging (BONCAT) to interrogate protein synthesis in veg

62 apply bioorthogonal non-canonical amino acid tagging (BONCAT) to visualize and quantify bacterial tra

63 When combined with endogenous locus GFP tagging by CRISPR-Cas9 or with rescue of a null mutant w

64 strate the sensitivity and specificity of EC-tagging by obtaining cell type-specific gene expression

65 oach, AAV-mediated anterograde transsynaptic tagging can categorize neurons by their inputs and molec

66 preparations for CT colonography with fecal tagging can improve patient comfort but may result in no

67 hes, and highlighted how fishery independent tagging can improve understanding of sailfish migrations

68 Here we report that tagging Cas9 with ubiquitin-proteasomal degradation sign

69 We report an alternative E3-E3 tagging cascade: many cellular NEDD8-modified CRLs assoc

70 Using activity-dependent tagging combined with neural manipulation techniques, we

71 differing by protein expression systems and tagging configurations.

72 Our vector series, pGTag (plasmids for Gene Tagging), contains reporters flanked by a universal CRIS

73 research as they are easy to use for protein tagging, cope without fixation or permeabilization, and

74 ned precursor isotopic labeling and isobaric tagging (cPILOT) for higher-throughput analysis of oxida

75 ned precursor isotopic labeling and isobaric tagging (cPILOT) is an enhanced multiplexing strategy cu

76 ) triangle was investigated using electronic tagging data from eight species that resulted in >22,000

77 esia, both which have historic telemetry and tagging data showing connectivity with Fijian foraging a

78 Fluorescent tagging demonstrated that two of the TpSAPs were localiz

79 "peekaboo" dynamics (0.8 Hz) in a frequency-tagging design.

80 The current study used a frequency-tagging EEG approach to separately measure responses to

81 smitter via injection has reduced the tag or tagging effect bias associated with studying small fishe

82 Moreover, tagging effects were stronger in smaller species.

83 naged to pinpoint key aspects and drivers of tagging effects.

84 endogenous expression of tagged genes, with tagging efficiency of up to 20% without selection, and u

85 Using an optical-tagging electrophysiological approach to record and labe

86 This study explores whether tagging endogenous proteins with a reporter is a scalabl

87 % of the identified T4-like viruses in viral tagging experiment infect Synechococcus, while only abou

88 onstrate an important step towards a 'barium-tagging' experiment, which identifies double beta decay

89 Translocation and tagging experiments revealed similar kelp mortality rate

90 Here, we use inducible RNA tagging experiments to show that immediately after trans

91 se results provide an empirical baseline for tagging experiments, life histories extrapolated from ot

92 high-pressure xenon gas detectors for barium-tagging experiments.

93 infectious viruses for a given host in viral tagging experiments.

94 true infectious associated viruses in viral tagging experiments.

95 eins, we used spatially restricted enzymatic tagging followed by mass spectrometry analysis of Caenor

96 This antibody is used for cell tagging followed by single-cell analysis using inductive

97 with electroencephalographic (EEG) frequency tagging following adaptation.

98 We performed isobaric tagging for relative and absolute quantification (iTRAQ)

99 An isobaric tagging for relative and absolute quantification (iTRAQ)

100 ear-envelope-targeting domain of the nuclear tagging fusion (NTF) protein with an outer nuclear-envel

101 The utility of the method was established by tagging genes encoding proteins of known localization su

102 Disruption of this 3' tagging has a significant but limited effect on histone

103 Based on these data we argue that mRNA 3' tagging has diverse and distinct roles associated with t

104 osyltransferase (UPRT), a method known as TU-tagging, has been used in multiple systems but can have

105 or extensive user intervention during sample tagging have posed barriers to the utilization of STOMP.

106 oduct isolation on all stages, whereby polar tagging helped.

107 ocalisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in thi

108 Here using RNAi, endogenous epitope tagging, immunofluorescence microscopy, and 3D-structure

109 Using reciprocal tagging in combination with affinity purification and ma

110 ty of LMO2 and its partners via Halo protein tagging in conjunction with variant proteins deficient i

111 ient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells.

112 esolution in humans depends on outcome value tagging in mPFC neurons influencing encoding of such val

113 Here, using activation tagging in the prime bioenergy crop poplar, we have iden

114 iogenesis preclude the use of simple genetic tagging in their cellular studies.

115 Neuronal genome tagging in vivo by Mef2c-Dam adenine methyltransferase f

116 For CL-activated tagging in vivo, tumor-bearing mice were injected first

117 tic exploration of cellular perturbations by tagging individual cells with RNA "barcodelets" to ident

118 After tagging individual ciliary transmembrane proteins, speci

119 In the present study, we found that EYFP tagging induces additional ubiquitylation of EYFP-CENP-A

120 In this work, we use messenger-tagging infrared (IR) spectroscopy to investigate the ge

121 ility spectrometry with cryogenic, messenger-tagging, infrared spectroscopy and mass spectrometry to

122 It is a known hot spot of retroviral tagging insertion and a fusion partner of both de novo a

123 Molecular tagging involves the selective modification of particula

124 Molecular tagging is an approach to labeling physical objects usin

125 where a neuronal instantiation of inhibitory tagging is seen.SIGNIFICANCE STATEMENT When we search a

126 Green fluorescent protein (GFP)-tagging is the prevalent strategy to monitor protein dyn

127 Epitope tagging is widely used to fuse a known epitope to protei

128 This memory enhancing effect (behavioral tagging) is caused by dopaminergic and noradrenergic neu

129 hanced cytoplasmic localization of RPW8.2 by tagging it with a NES led to lethal cell death.

130 Over the last ten years, isogenic tagging (IT) has revolutionised the study of bacterial i

131 We used quantitative isobaric tagging (iTRAQ) proteomics to determine the L. rostrata

132 multifaceted approach to investigate whether tagging leads to short-term behavioural changes, and whe

133 CT compared with conventional images on all tagging levels (P < .001).

134 ared with conventional CT at different fecal tagging levels in vitro.

135 BS) conversion, preamplification and adaptor tagging, library amplification, sequencing and, lastly,

136 It turns out that the GFP tagging location and the fusion protein stability have a

137 and typically traveled farther from initial tagging locations.

138 hieving median displacement of 1,057 km from tagging locations.

139 ocalization of organelle proteins by isotope tagging (LOPIT), which combines biochemical cell fractio

140 We proposed that selective labeling/tagging may improve base-to-base resolution of nucleic a

141 hat this behavior is driven by an inhibitory tagging mechanism that inhibits responses on priority ma

142 Our results uncover a proteolytic-tagging mechanism with implications toward the function

143 No molecular tagging method exists that is inexpensive, fast and reli

144 Multiplexed, isobaric tagging methods are powerful techniques to increase thro

145 lopment but also is central to other protein-tagging methods in modern chemical biology including act

146 this review, the effectiveness of molecular tagging methods incorporating carbon, silicon, nitrogen,

147 Some potential tagging methods which have not yet been applied to NOM a

148 th and without ADHD suggests that behavioral tagging might only be able to improve memory consolidati

149 measured by using a T2-relaxation-under-spin-tagging MRI technique with a 3.0-T MRI system.

150 mapping that displayed reduced fibrosis, and tagging MRI that showed improved regional myocardial str

151 rm this strategy mass difference tandem mass tagging (mTMT). These TMT variants differ by 11 mass uni

152 We then utilized an activity-dependent tagging murine line, the ArcCreER(T2) mice, to express c

153 reening, we identified a dominant activation-tagging mutant, fiberless-d (fls-d), showing defective S

154 Tagging N-terminal fragments of TMC1 with Aquaporin 3 (A

155 Tools for tagging nucleic acid sequences and reporter molecules th

156 We show that 'EC-tagging' occurs in tissue culture cells and Drosophila e

157 Affinity and epitope tagging of carbon was achieved using direct attachment o

158 rt a molecular technology for stable genetic tagging of cells that exhibit activity-related increases

159 Light activated photo-ODIBO enabled tagging of cellular RNA, in addition to fluorescent imag

160 gene expression, as well as for fluorescence tagging of chromosomal regions and individual mRNAs to t

161 tivations of multifocal activities, allowing tagging of complex representations necessary for REM sle

162 Epitope tagging of endogenous Na(V)1.7 revealed the channel to b

163 , based on lineage-specific knockout and GFP-tagging of endogenous pop-1, support the model that POP-

164 Using this strategy, we achieved efficient tagging of endogenous proteins in primary and organotypi

165 re we describe a versatile toolset for rapid tagging of endogenous proteins.

166 Here we use conditional tagging of FMRP and CLIP (FMRP cTag CLIP) to examine FMR

167 Here, we combined fluorescent tagging of gamma-secretase subunits with super-resolutio

168 Our data show for the first time that FlAsH-tagging of ion channels is a promising tool to study con

169 GFP tagging of LIMP caused a limping defect during movement

170 ducible method for cell-type-specific biotin tagging of MeCP2 in mice.

171 lls in mice that we validated by optogenetic tagging of mossy cells.

172 rtner-specific knowledge, over and above the tagging of motor routines with ostensive cues.

173 Moreover, endogenous tagging of Nanog in embryonic stem cells reveals that ER

174 Here we combine genetic tagging of nuclei and ribosomes with RNA sequencing, chr

175 eveloped a method that we designate isotopic tagging of oxidized and reduced cysteines (iTORC).

176 The genomic tagging of PALM fluorophores through CRISPR-Cas9 offers

177 In addition to efficient tagging of proteins in vivo, this HDR methodology will a

178 Here, using RNAi, in situ epitope tagging of proteins, GST pulldown, and coimmunoprecipita

179 Tagging of SipA with the small fluorescent phiLOV tag, w

180 olutionized cell biology by allowing genetic tagging of specific proteins inside living cells.

181 face protein capture is mediated by covalent tagging of surface glycans, yet current methods do not a

182 One solution involves biosynthetic tagging of target RNAs.

183 ated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish.

184 otype in the sporozoite arising from epitope tagging of the endogenous protein) as a key regulator fo

185 investigate the effect of C- and N-terminal tagging of the luminal ATPase torsinA on its ability to

186 the developed methodology was applied to the tagging of the most famous member of this family of comp

187 s reveals enrichment of human-specific m(6)A tagging of transcripts related to brain-disorder risk ge

188 Both lack of power, and joint tagging of two or more distinct causal variants by a sin

189 usefulness of this strategy in differential tagging of wild-type and mutant cells in mosaics.

190 We calculated the effect of tagging on apparent survival, condition, phenology and b

191 viduals to examine the effects of geolocator tagging on small bird species (body mass <100 g).

192 how strong position-dependent effects of Cy5-tagging on the structure and natural dynamics of membran

193 By tagging one axis protein (HIM-3) with a photoconvertible

194 ion (OOPS), which does not require molecular tagging or capture of polyadenylated RNA, and apply it t

195 evelopment of modern immunosensors, both for tagging or modifying electrode transducers, are summariz

196 OOPS avoids molecular tagging or the capture of polyadenylated RNA.

197 During the late tagging period, Chukchi belugas had significantly delaye

198 n 'early' (1993-2002) and 'late' (2004-2012) tagging periods.

199 esent FucoID, a glycosyltransferase-mediated tagging platform, to biochemically label and capture ant

200 nalogous anterograde transsynaptic tools for tagging postsynaptically targeted neurons remain under d

201 cle, and neuronal differentiation, and m(6)A tagging promotes their decay.

202 In all, our data indicate the intricacy of tagging proteasomes, and possibly, large complexes in ge

203 However, tagging proteins at their endogenous loci results in chr

204 By tagging proteins expressed in C. trachomatis with OVA(32

205 demonstrate the flexibility of the system by tagging proteins with distinct cellular localisations.

206 However, tagging proteins with FPs is not without problems: forma

207 We demonstrate that tagging proteolytic fragments with mass sensitivity prob

208 t was sequenced using Primer ID, an amplicon-tagging protocol, which is designed to reduce errors and

209 Broccoli aptamer-tagging provides a valuable tool for live cell imaging o

210 EC-tagging provides several advantages over existing techni

211 hod named quantitative O-propargyl-puromycin tagging (QOT) by integrating O-propargyl-puromycin (OPP)

212 amplification with random PCR amplification tagging (RCA-RA-PCR), high-throughput sequencing (Illumi

213 f methionine called redox-activated chemical tagging (ReACT) but have not broadly tested the molecula

214 er partners through Redox Activated Chemical Tagging (ReACT).

215 ing the sensitivity approach and 0.5 ppbv by tagging reactive nitrogen oxides.

216 alternative to expensive commercial isobaric tagging reagents, we developed our own custom N,N-dimeth

217 onsequently, realization of large-scale gene tagging requires further development of approaches to ge

218 present prokaryotic expression profiling by tagging RNA in situ and sequencing (PETRI-seq)-a low-cos

219 urce of feedback that creates the inhibitory tagging seen in parietal cortex.

220 We developed physiological optical tagging sequencing (PhOTseq), a technique for tagging an

221 nt single-cell metabolically labeled new RNA tagging sequencing (scNT-seq), a method for massively pa

222 polymerase chain reaction using a quadruple-tagging set of primers specific for E. coli eaeA (151bp)

223 by cells during cell division, which enables tagging several generations of cells.

224 We hypothesized that this inhibitory tagging signal should be represented as an elevated resp

225 eurons could be the source of the inhibitory tagging signal to parietal cortex, where a neuronal inst

226 We also show that by redundantly tagging single molecules with multiple, blinking fluorop

227 ), effect sizes, linkage disequilibrium with tagging single nucleotide variants used in GWAS, and lik

228 crease in alignment as the distance from the tagging site increases.

229 species and the number of published studies tagging small birds has increased substantially.

230 A total of 31 tagging SNPs associated with the TG response were used f

231 We performed an association study using 6 tagging SNPs, (rs860170, rs978739, rs1357949, rs1525489,

232 ip' (ADPC), a genotyping array consisting of tagging SNPs, useful in comprehensively identifying Afri

233 by summing the number of at-risk alleles of tagging SNPs.

234 n be engrafted thanks to widely used genetic tagging strategies.

235 o a lack of in vivo, spleen-specific lineage tagging strategies.

236 clients using a ubiquitin-mediated proximity tagging strategy and show that, despite their high degre

237 so map base J loci by introducing a chemical tagging strategy for the glucopyranoside residue.

238 ng of 5hmU-modified loci based on a chemical tagging strategy for the hydroxymethyl group.

239 ere we report a preribosome purification and tagging strategy that overcomes some of the existing tec

240 ed experimental approach chosen in most gene tagging studies because of its time efficiency and acces

241 uals from 98 populations of 45 species, from tagging studies in the Neotropic and Afro-Palearctic fly

242 Tagging studies suggest that while offshore, white shark

243 A fluorescent protein-tagging study pointed to a peroxisomal location of CrACX

244 However, errors before UMI tagging, such as DNA polymerase errors during end repair

245 Moreover, by tagging SV proteins with a light-activated ROS generator

246 multifunctional capabilities of this protein-tagging system also permit in vivo validation of interac

247 we present Porcupine, an end-user molecular tagging system featuring DNA-based tags readable within

248 In this study, we used an activity-dependent tagging system in mice to determine the epigenetic state

249 We have developed a recombineering-based tagging system that brings together the convenience of t

250 t is an extensible, real-time, high accuracy tagging system that includes an approach to developing h

251 teractions and an allelic RNA-depletion and -tagging system.

252 (2019) introduce new orthogonal peptide-tagging systems to study translation in multiple reading

253 Using a chemical tagging target engagement biomarker assay, we identify t

254 Here, epitope-tagging Teb2 and Teb3 expressed at their endogenous gene

255 s and to their interaction using a frequency-tagging technique.

256 elopment of satellite, archival and acoustic tagging techniques that allow the tracking of marine ani

257 Alternatively, TF-tagging techniques, in which a TF is fused to a DNA-modi

258 uture studies and outline various aspects of tagging that need further investigation.

259 capped RNA using a strategy of specifically tagging the 5' end of capped RNA by first decapping and

260 ith large effects on age-specific mortality: tagging the APOE epsilon4 allele and near CHRNA3.

261 cently tagged, recombinant proteins and also tagging the DLP, we distinguished particles that have lo

262 ligand binding domain of ESR1, while epitope tagging the endogenous locus.

263 rst screen for candidate binding partners by tagging the extracellular and cytoplasmic regions of a "

264 CRHR1, p=4.91x10(-7)) with the effect allele tagging the H1 haplotype.

265 es and aberrant tRNA substrates, selectively tagging the latter for degradation.

266 F-SIMS images, describing a method for color-tagging the output of a self-organizing map (SOM).

267 These SNPs potentially tagging the Sr38 gene could be used in marker-assisted s

268 We present a method for color-tagging the toroidal SOM output, which reduces the entir

269 5L (long) and AtUPS5S (short) was studied by tagging them with fluorescent proteins in their cytosoli

270 f allele-sequenced and PCR validated markers tagging these QTLs is immediately applicable for marker-

271 mple preparation procedures (e.g., molecular tagging) through the combination of single-cell printer

272 and metabolic labeling, namely, tandem mass tagging (TMT) and SILAC, are among the most widely used

273 ILAC measurements, combined with tandem mass tagging (TMT) or not, would greatly benefit from instrum

274 plasma using brain tissue-enhanced isobaric tagging (TMTcalibrator).

275 challenges by using noncanonical amino acid tagging to fluorescently label extracellular matrix synt

276 we combine CRISPR genome editing and MS2 RNA tagging to image single molecules of telomerase RNA (hTR

277 e present the first application of frequency tagging to intracellular neuronal recordings, demonstrat

278 We used activity-dependent neural tagging to investigate representations of fear and extin

279 peptide MHCs (hipMHCs) and multiplex isotope tagging to quantify peptide repertoire alterations using

280 NCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soil

281 proaches, including NMR, MS, and fluorescent tagging, to study the redox function of Grx1, the only c

282 ithin many droplets in parallel, DNA barcode tagging together with the mRNA molecules from the same c

283 synaptic tagging and capture and behavioral tagging, two major mechanisms of associative learning an

284 n the results obtained by direct chromosomal tagging using genome-editing strategies.

285 d quantified the phosphoproteome by isobaric tagging using tandem mass tag liquid chromatography/tand

286 n Europeans and 22% in West Africans for the tagging variant) as well as another haplotype near the F

287 portability by prioritizing functional over tagging variants.

288 RNA tagging via incorporation of 4-thiouracil (TU) in cells

289 ealed statistically significant differences (tagging vs. blood myocardial border tracing algorithm).

290 ons, an RNA fragmentation step before biotin tagging was introduced, in an approach known as transien

291 onal, cFos-based genetic manipulations ("Fos tagging"), we show that olfactory fear conditioning acti

292 ng translin/trax display defects in synaptic tagging, which requires protein synthesis at activated s

293 ining extracellular and cytoplasmic proximal tagging with a biophysical binding assay increases the p

294 emical labeling, allowing efficient chemical tagging with a reduced excess of labeling reagent withou

295 , we identify positions that are amenable to tagging with an environmentally sensitive reporter group

296 or potential drug targets, were targeted for tagging with HiBiT in multiple cell lines.

297 Based on the widely used technique of RNA tagging with MS2 aptamers for RNA visualization, we deve

298 We propose that behavioral tagging with novel virtual environments is a promising c

299 research, we investigated whether behavioral tagging with novelty can be used to tackle memory proble

300 e demonstrated dual-color endogenous protein tagging with sfCherry211 and GFP11, revealing that endop