ライフサイエンスコーパス: target site

1 dCas9 effectors is highly selective for the target site.

2 on needed for its interaction with the viral target site.

3 ate Cas9 catalytic activity at the specified target site.

4 large genomic sequence loss around the gRNA target site.

5 hanged and many miRNAs from wild mice gained target site.

6 also contained mutations at the integration target site.

7 ed the loss of DNA sequences around the gRNA target site.

8 s9 induces the formation of an R-loop at its target site.

9 o 100 kilobases, are created upstream of the target site.

10 uplex that perfectly matches the protospacer target site.

11 of AAV integration except at the CRISPR/Cas9 target site.

12 on time and poor local concentrations at the target site.

13 get RNAs by guiding Argonaute2 (AGO2) to its target site.

14 s a high affinity and long dwell-time at its target site.

15 ry a single copy of full-length T-DNA at the target site.

16 assembly into nanoaggregates retained at the target site.

17 nd specificity of integration at the genomic target site.

18 entrate and activate the microbubbles at the target site.

19 de an effective mean of drug delivery to the target site.

20 ioavailability and controlled release at the target site.

21 release of the drug for full activity at the target site.

22 ve C-to-T and A-to-G conversions at the same target site.

23 vity, including nick generation, at specific target sites.

24 and essentially no insertions at all tested target sites.

25 o achieve consistent BBB opening at multiple target sites.

26 partner proteins to recognize their genomic target sites.

27 ism that restricts Ca(V) 1.2 activity to its target sites.

28 much higher editing efficiency at difficult target sites.

29 targeted DNA damage but can also affect off-target sites.

30 ning xCas9 failed to edit most of the tested target sites.

31 length and fragmented AAV genomes at Cas9 on-target sites.

32 then whole-genome sequenced to identify off-target sites.

33 e-wide DNA insertion across dozens of unique target sites.

34 n-specific cassette containing all potential target sites.

35 and reliable detection of relevant enzymatic target sites.

36 lation by integrating synthetic promoters at target sites.

37 its high abundance and the frequency of its target sites.

38 ranscription factors (TFs) and their genomic target sites.

39 ong background signals due to the increasing target sites.

40 is of the cervical epithelium than for other target sites.

41 ice to search AS regions harboring small RNA target sites.

42 ectively reduce activity at low-affinity off-target sites.

43 editing than Cas9 nuclease at known Cas9 off-target sites.

44 binding sites, and microRNA genes and their target sites.

45 herit and maintain DNA methylation at ZFP708 target sites.

46 ciency including structured 5'UTRs and miRNA target sites.

47 ach contribute to directing PRC2 activity to target sites.

48 heptad repeats at serines 5 and 7, the known target sites.

49 associated memories ('cocktail') in cortical target sites.

50 mutations through similar mechanisms at off-target sites.

51 concentrations of therapeutic agents at the target sites.

52 ssful in generating desired mutations at the target sites.

53 ation among miRNAs when attempts to discover target sites.

54 onality precisely matched to homo-oligomeric target sites.

55 e homotetramer containing a pair of loxP DNA target sites.

56 RNAs (gRNAs) designed to induce loops at the target sites.

57 enes by randomly introducing errors into the target sites.

58 umerate a comprehensive list of putative off-target sites.

59 hat impose stringent requirements on CRISPRa target sites.

60 cating that these are primary ubiquitination target sites.

61 ural variation events are enriched at the on-target sites.

62 redictive rules for designing effective gRNA target sites.

63 s in anatomy or are not generalizable across target sites.

64 ically displays enriched RNA-binding protein target sites.

65 (miRNAs) targets and accurately locate their target sites.

66 dency to cleave DNA non-specifically at "off-target" sites.

67 the efficient delivery of drugs and genes to targeted sites.

68 acquisition that was mostly centered on the targeting site.

69 e Pho7 DNA-binding domain (DBD) bound at its target site 2 in the pho1 promoter (5'-TCGGAAATTAAAAA).

70 o achieve efficient and specific cleavage at target sites, a high degree of target site discriminatio

71 Despite their non-overlapping target sites, a network analysis revealed that some FIKK

72 ining genome-wide RIsearch2 predictions with target site accessibilities and transcript abundance est

73 itical for AGO2-target interactions, but how target site accessibility is controlled in vivo is poorl

74 Target site accessibility is critical for AGO2-target in

75 or DNA and the presence of tandem repeats at target sites, achieving replacement with up to 130-bp se

76 aneous multiplex base editing of up to three target sites across 11 genes/loci in cynomolgus monkey e

77 We found high variation in the density of L1 target sites across human protein-coding genes.

78 RNA, miRNA-specific differences in canonical target-site affinities, and a 100-fold impact of dinucle

79 Potential drive-resistant target-site alleles arise at a frequency <0.1, and five

80 ificant effects on activity at ~15.2% of off-target sites analyzed.

81 a loop that interacts with the transposition target site and an accordion-like C-terminal tail that e

82 s to allow controlled linker cleavage at the target site and are thus termed 'cleavable'.

83 s9-fused epigenome modifier complex from the target site and enables temporal control over gene expre

84 g guide RNA (pegRNA) that both specifies the target site and encodes the desired edit.

85 Yorkie, S74 and S97, are Atg1/ULK1 consensus target sites and are phosphorylated by ULK1 in vitro, th

86 ere connected to EAM, navigated to tagged HB target sites and deployed.

87 ies of sgRNAs containing mismatches to their target sites and derived rules governing mismatched sgRN

88 ichment in co-occupancy with PBX proteins on target sites and exists in the same complex with PBX on

89 /deletions (indels) can result in altered on-target sites and novel potent off-target sites, which ca

90 It causes significant material loss to off-target sites and poor availability at the intended deliv

91 a result of DSBs at allelic and non-allelic target sites and report that simultaneous single-strande

92 de the first comprehensive resource of miRNA target sites and their quantitative functional impact in

93 The variant creates a miR-382-5p targeting site and compromises a miR-325-3p site.

94 rotospacer adjacent motif (PAM) flanking the target site, and subsequent R-loop formation and strand

95 d be used together to identify potential off-target sites, and amplicon-based next-generation sequenc

96 eotides having 5'TC3' or 5'CT3' dinucleotide target sites, and different flanking bases within divers

97 s essential for proteins searching for their target sites, and protein diffusion on microtubules is i

98 og (Nanog homeobox) and Hoxa1 on many common target sites, and these are linked to genes in the pluri

99 nalysis of downregulated genes yielded Sirt1 target sites, and we observed reduced Sirt1 activity in

100 penetrability, specific accumulation in the targeted site, and enhanced therapeutic efficacy.

101 in vitro-based methods and that on- and off-target sites are detectable in gene bodies where short-r

102 with ABE, 2-19 (with an average of 8.0) off-target sites are found, significantly fewer than those f

103 ted that the only functionally critical PLK1 target sites are in a single cluster in the CYK4 N termi

104 Confirmed target sites are nearly always in highly conserved, prot

105 or a large body of kinases, only a few or no target sites are reported.

106 Cleaved target sites are then selectively PCR amplified.

107 sion of imprecise editing outcomes at the on-target site as key design parameters that control the ef

108 m and efficient delivery of nanoparticles to target sites as well as physiological stabilization of n

109 A putative kinase target site at Y265 in the actin binding domain was also

110 is widely utilized gene-editing tool selects target sites, avoids spurious DNA cleavage activity, and

111 e low efficiency of drug transmitting to the target sites because of the impedance of complex biologi

112 avage but not miRNA binding, thus decoupling target site binding from cleavage.

113 ntral infusion during the juvenile period of target site blockers, tailored to prevent miR-30 binding

114 reduce H3K27me3 proportionately at all PRC2 target sites, but ~40% uniform residual levels keep targ

115 Cas9 recognizes its target site by unwinding the DNA double helix and hybrid

116 will help users to identify functional piRNA target sites by evaluating various information.

117 e editor variants were more precise at their target sites by producing fewer multiple C edits.

118 NA cleavage frequently involves unmasking of target sites by translating ribosomes.

119 arallel assessment of polymorphisms in miRNA target-sites by sequencing) assay, we identified 25 SNPs

120 CRISPR GUARD) as a method for protecting off-targets sites by co-delivery of short guide RNAs directe

121 e sequence that flanks the 3' end of the RNA target site (called the PFS).

122 so viability, showing that a single microRNA target site can be essential.

123 Here, we report that off-target sites can be shielded from the active Cas9*single

124 ive targets, while minimizing binding to off-target sites, can translate to improved drug efficacy an

125 the adeno-associated virus vector into Cas9 target sites caused premature termination of Ube3a-ATS a

126 ors flanked by 100 nt homology arms and gRNA target sites cloned into a plasmid.

127 n enhanced concentration of 6-shogaol at the target site (colon), this concentration is still far bel

128 cacy is driven by gRNA-specific features and target site context.

129 peculate that aberrant methylation of DNMT3B target sites could contribute to the oncogenic potential

130 get deamination is further validated through target site deep sequencing.

131 onal editing accuracy within cells, and high target site density for in vivo genome editing applicati

132 cleotide PAM (N(4)CC) that provides for high target site density.

133 Genome-scale experiments for miRNA target site detection are still costly.

134 c cleavage at target sites, a high degree of target site discrimination must be demonstrated for gene

135 Here, we compare TE target site distribution in host genomes using multiple

136 on the toxicodynamics of the chemical (e.g., target site distribution).

137 Target site DNA base unstacking, flipping, and melting b

138 ransfer complex that differ based on whether target site DNA is annealed or dynamic.

139 ed vertebrate-specific loop to interact with target site DNA, and functional assays demonstrate that

140 lly demonstrate the suitability of synthetic target site drives as well as split drives as flexible s

141 They produce a three-base target site duplication and do not have homology to othe

142 TE identifies the hallmarks (poly-A tail and target site duplication) and orientation of Alu insertio

143 SSRP1) in governing Cas9 turnover at the DNA target site during genome and epigenome editing.

144 lymphocytes (CTLs) are thought to arrive at target sites either via random search or following signa

145 1AX Evolutionary loss of a Y-linked microRNA target site enabled up-regulation of EIF1AY, but not of

146 n primary T cells and identified 201,934 off-target sites, enabling the training of a machine learnin

147 experimental annotation of functional miRNA target sites exists in Drosophila.

148 inetic models were used to estimate internal target site exposure from external exposures.

149 Effective target sites fall within two domains, which are conserve

150 The Si site offers a novel target site for allosteric inhibitors and a molecular ex

151 e TRPV2 channel, also recently proposed as a target site for CBD.

152 The target site for miR-346 overlaps with active sites for a

153 lusion, these findings not only identified a target site for the development of CaTMPK-selective drug

154 s highlights the medial parietal cortex as a target site for transforming neural activity into contro

155 port a set of unique de novo DNA methylation target sites for both DNMT3 enzymes during mammalian dev

156 repeat (PPR) proteins specifically recognize target sites for C-to-U RNA editing in the transcriptome

157 ons includes ubiquitous polymorphisms within target sites for Cas9-based gene drive (CGD) and that th

158 However, its preferential target sites for DNA methylation are largely unknown.

159 e RNA tunnel in DENV RdRp offers interesting target sites for inhibition.IMPORTANCE Dengue virus (DEN

160 ular RNA-RNA interactions, which can conceal target sites for many minutes in the absence of translat

161 The truncation leads to a loss of target sites for microRNAs known to repress translation

162 , transport pathways and accumulation at the target sites for particular molecules.

163 rotein-coupled receptors (GPCRs), common off-target sites for piperazine-containing D(3)R scaffolds.

164 X-AUG motifs and is predicted to function as target sites for the 18S rRNA by direct base pairing.

165 spots in some kinases and thus are potential target sites for type IV inhibitors.

166 p to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.

167 is mARF6 or mARF8 plants with mutated miR167 target sites have defective anther dehiscence and ovule

168 ndidates to identify recurrent SVs and their targeted sites (hotspot regions), visualizes these genom

169 RBP binding at specific target sites impacts expression of functionally coordina

170 ost prevalent polymorphisms in the guide RNA target site in collections of colonized and wild-derived

171 t four such conversions at the mitochondrial target site in other taxa, AEF1 remains consistently con

172 racrRNA enhanced indel formation at the CCR5 target site in primary CD4+ T-cells.

173 t RXFP1 and identified a putative miR-144-3p target site in the RXFP1 mRNA.

174 ing events appear repeatedly at the same off-target sites in a guide-RNA-sequence-dependent manner, d

175 f nuclear factor kappa B (NFkappaB) from DNA target sites in a process we have termed molecular strip

176 our knowledge, the overall profile of miRNA target sites in circular RNAs (circRNA) generated by alt

177 ntegrated target sequences and 78 endogenous target sites in human cells.

178 d translocation frequency to IGH and AID off-target sites in human chronic lymphocytic leukaemia and

179 identify thousands of potential Cas13 crRNA target sites in hundreds of ssRNA viral species that can

180 We conclude that the abundance of conserved target sites in mosquito genomes and the inherent flexib

181 domain using substrate pools containing CpX target sites in randomized flanking context and identifi

182 IC-seq reliably detects H3K4me3 and H3K27me3 target sites in single human white blood cells.

183 ribozymes are readily designed for specific target sites in small and large RNAs and accept a wide v

184 A methyltransferases interact with their CpG target sites in the context of variable flanking sequenc

185 effects on TR and imply that other potential target sites in the thyroid hormone axis should be a gre

186 rived microRNA, miR-1-3p, that has predicted target sites in the transformer gene (Bdtra) required fo

187 of SAUR63 subfamily genes through conserved target sites in their promoters.

188 FOXH1 is prebound to target sites in these loci and recruits SMAD3 independen

189 or chromosomal DNA fragment insertion at the target sites in transgenic events generated from both bi

190 Transcription factor binding to target sites in vivo is a dynamic process that involves

191 s could preferably methylate DNMT3B-specific target sites in vivo.

192 ol of Cas9 activity over dosage, timing, and targeted site in an organism.

193 Ser(315) is a known PKC-targeted site in flotillin-1.

194 situ We found the inverted ORF57 gene in the targeted site in the KSHV genome in one of two character

195 ShCAST integrates DNA into targeted sites in the Escherichia coli genome with frequ

196 ell transplantation from in vitro culture to targeted sites in vivo.

197 redicted and experimentally identified piRNA targeting sites in Caenorhabditis elegans.

198 indicated the effective payloads towards the target site, increased apoptosis in cancer cells and imp

199 calcification pattern was associated with VT target sites independent of calcification volume ( P=0.0

200 complexes with DNA-binding activities occupy target sites independently of PRC1 catalytic activity, p

201 ion, and chromatin accessibility at RARalpha target sites, independently of the downstream, RAR-media

202 s well as direct sequencing of genomic sgRNA target sites, indicates that the vast majority of transg

203 ilico that the path conferred resistance and target-site insensitivity to cardiac glycosides(16), cul

204 Only one predicted off-target site is cleavable in vitro, with negligible delet

205 Its target site is the alpha6 subunit of the nicotinic acety

206 o address the challenge, we identified miRNA target sites located in alternatively spliced regions of

207 ong them, there were 64 781 and 41 146 miRNA target sites located in linear and circular AS region, r

208 r for deposition and identification of miRNA target sites located in the alternatively spliced region

209 Target site masking is caused by heterogeneous intramole

210 ndan C. quinquefasciatus is mediated by both target-site mechanisms and over-expression of detoxifica

211 dings represent the first documented case of target-site mediated glyphosate resistance in horseweed

212 Target sites might carry genetic variations that are not

213 nucleotides 15-21 are largely intolerant of target site mismatches.

214 hether acaricides with similar physiological target site/mode of action also elicit similar behaviora

215 Genotyping of the G119S Ace-1 target site mutation detected a highly significant assoc

216 in comparison to the A296S-RDL target site mutation.

217 o glyphosate, and at least 13 species have a target-site mutation at position 106 of EPSPS.

218 m Cordoba province was mainly due to a P106S target-site mutation in the 5-enolpyruvylshikimate 3-pho

219 lso contains an unreported Pro-106-Thr EPSPS target-site mutation.

220 resistance in clinical isolates is caused by target site mutations in penA, some isolates lack these

221 wo nucleotides immediately downstream of the target site, named Target Adjacent nucleotide Motif, can

222 ile only weakly correlated with absolute off-target site number, could be predicted by the recently d

223 ed kinase specificity models, based on known target sites, observing that specificity has remained mo

224 Mutations in the miR172 target site of AP2L2 were associated with reduced plant

225 accine priming of immune responses against a target site of vulnerability, followed by vaccine boosti

226 or studying functional genomics of important target sites of anthelmintics have been restricted to Ca

227 r-677, Ser-705, Ser-748, and Ser-774) as the target sites of CKI.

228 profiling approaches, we identify the direct target sites of E2A-PBX1 in t(1,19)-positive pre-B ALL c

229 get site Selection) that can screen putative target sites of miRNA-target binding.

230 Nem1 and Ser-22/Ser-28 in Spo7 are major PKC target sites of phosphorylation.

231 of lineage-specific genes in B cells are off-target sites of somatic hypermutation.

232 also mediated ORF57 inversion in situ in the targeted site of the KSHV genome.

233 entify dosage-sensitive CHD8 transcriptional targets, sites of regulated accessibility, and an unexpe

234 DNA sequence and shape determinants of MEF2B target sites on a high-throughput basis in vitro for wil

235 by directing other molecules to reach their target sites on collagens.

236 nes of interest and retrieve a list of piRNA targeting sites on the input genes.

237 either increase the concentration of PRC2 at target sites or inhibit the rate that PRC2 samples chrom

238 variability, leading to either indels at 1-2 target sites or inter-target deletions.

239 ospacer adjacent motif (PAM) next to the DNA target site plays a crucial role in determining both eff

240 w L1 insertions bore structural hallmarks of target-site primed reverse transcription (TPRT) and mobi

241 d antidromic activation between animals, and target sites, raise questions about its hypothesized rol

242 a protospacer-adjacent motif (PAM), limiting target site recognition to a subset of sequences.

243 l thicknesses and intraocular pressures, and target sites relevant for epidural injections, subcutane

244 Both target-site resistance (TSR) and nontarget-site resistan

245 g observed at the donor loci and the genomic target site, respectively.

246 nables gene tracking and fine control of the target site's basal occupancy.

247 rvations provide new insights into A3 enzyme target site selection and how A3 mutagenesis impacts mut

248 concept that it is possible to influence the target site selection of SB by fusing it to a catalytica

249 ored Dynamic Programming algorithm for miRNA target site Selection) that can screen putative target s

250 providing a putative mechanism for Polycomb target site selection.

251 w mechanism by which protein partners refine target site selection.

252 dependent and newly replicated chromatin at target sites shows marked nucleosome depletion when CAF-

253 otential and 3' versus 5' placement of miRNA target sites significantly affect the PB localization dy

254 This approach revealed noncanonical target sites specific to each miRNA, miRNA-specific diff

255 GGACATTCAAAT) reveals shared determinants of target site specificity as well as variations in the pro

256 Instead, we found that the unfolding of the target site structure plays a key role in miRISC activit

257 terial drugs that act at the major bacterial target sites such as the ribosome, penicillin-binding pr

258 apeutic DBS identified an optimal anatomical target site that can help surgeons to guide their surgic

259 detected aberrant integration events at the target site that dramatically and inaccurately shifted h

260 cid treatment, Hoxa1 is rapidly recruited to target sites that are associated with genes involved in

261 vity is specifically directed to a subset of target sites that are stably occupied by the complex and

262 aracterize unintended larger deletions at on-target sites that frequently evade common genotyping pra

263 nnotate the potential impact of putative off-target sites that lie within regions of functional genom

264 solvent exchange and relaxation rates of the targeted sites; these gains also benefit considerably fr

265 bond hydrolysis within or close to their DNA target sites, they form different oligomeric assemblies

266 ure transcription factor binding dynamics at target sites throughout the human genome.

267 lication, etc.) can expose these cryptic off-target sites to Cas9 activity, highlighting the need for

268 inally, by linking distinct mSWI/SNF complex target sites to tumor-suppressive gene expression progra

269 a model, we discovered that a nonfunctional target site unable to base-pair extensively with the miR

270 1 subunit is preferentially recruited to the target sites upon RA or E(2) induction of transcription.

271 n, which occurs when introns spread to empty target sites via homologous recombination.

272 s N-terminal peptide (encompassing the Lys-5 target site), we generated an EftM homology model and us

273 To sample the scope of cognate DB1976 target sites, we evaluated three dodecameric duplexes sp

274 ircuits for 49 VTs (33% of all VTs for which target sites were identified) in 24 of 39 patients (62%)

275 This approach revealed that miRNA target sites were not structurally accessible for miRISC

276 MGB assay was up to 25% divergent across the target sites when aligned to the Liberian Lassa virus ge

277 Yki shows greater association with target sites when CAF-1 is depleted and misregulation of

278 CPP can enhance delivery of drugs to a targeted site when combined with tumor targeting peptide

279 altered on-target sites and novel potent off-target sites, which can predispose patients to treatment

280 so that functional proteins can access their target sites, which otherwise are sterically occluded.

281 iverged rapidly, except for the miR2118/2275 target sites, which were under strong selection for cons

282 silences TUSC2 by binding to a unique 5'-UTR target-site, which overlaps with the translation start-s

283 in natural populations include at least one target site with no DRAs at a frequency of >=1.0%.

284 ergy creates a temperature gradient around a target site with temperatures known to create tissue inj

285 the unique capability of binding to its DNA target sites with a degenerate motif, while still functi

286 ed antidromic activation between animals and target sites with antidromic activation not observed dur

287 at xCas9 can efficiently induce mutations at target sites with NG and GAT PAM sequences in rice.

288 ining either different promoters, or altered target sites with varied levels of guanine-cytosine base

289 that can cross the BBB and deliver drugs to targeted sites with high drug-loading efficiency and lon

290 ds to a decrease in pseudouridylation of its target site within the E-site transfer RNA (tRNA) bindin

291 is necessary to enumerate all potential off-target sites within a given genome that could be inadver

292 In cells, closely spaced microRNA (miRNA) target sites within a messenger RNA (mRNA) can act coope

293 gene expression is primarily associated with target sites within coding sequences instead of 3'UTRs.

294 te gene expression through interactions with target sites within mRNAs, leading to enhanced degradati

295 oach, we identify miRNA-specific activity of target sites within the open reading frame.

296 ylation at Ser-845, a protein kinase A (PKA)-targeted site within its intracellular C-terminal tail,

297 igh antineoplastic drug concentration at the target site without damaging healthy tissues.

298 gh concentration of the drug directly to the target site without the associated systemic toxicities.

299 e C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks.

300 verse genomic alterations to be written into target sites without requiring double-strand breaks or d

301 Mutations induced within a MO target site would result in a Morpholino-refractive alle