ライフサイエンスコーパス: tegument

1 t-parasite interface, a structure called the tegument.

2 model of the protein organization inside the tegument.

3 to cytoplasmic membranes and into the virion tegument.

4 esent in mature virions as components of the tegument.

5 ovel drugs targeting TEMs in the schistosome tegument.

6 tructural changes in the treated F. hepatica tegument.

7 connecting the inner tegument with the outer tegument.

8 the activated form of ERK2 (pERK2) into the tegument.

9 ICP0 results in the absence of ICP0 from the tegument.

10 have the potential to reach all parts of the tegument.

11 rtant for the structural organization of the tegument.

12 p2) are expressed in the Schistosoma mansoni tegument.

13 ion of Ov-TSP-2 and TSP-3 to the adult fluke tegument.

14 The major tegument 150-kDa phosphoprotein (pp150) of HCMV binds to

15 eins, most of which are contained within the tegument, a complex structural layer between the nucleoc

16 hin a proteinaceous capsid surrounded by the tegument, a layer of viral and cellular proteins.

17 the oral sensory papillae, acetabular ducts, tegument, acetabular glands, and nervous system.

18 sights into the poorly understood process of tegument acquisition.

19 importance of pUL36 in the initial stages of tegument addition and provides new insights into the pro

20 nses specific to soluble worm antigen (SWA), tegument allergen-like 1, and 28-kDa glutathione-S-trans

21 dral nucleocapsid surrounded by an amorphous tegument and a lipoprotein envelope.

22 ts icosahedral symmetry, but the surrounding tegument and envelope layers lack regular architecture.

23 An analysis of 11 tegument and envelope proteins defined the composition a

24 ly investigated, the precise organization of tegument and envelope proteins remains elusive.

25 me, followed by acquisition of a pleomorphic tegument and envelope.

26 had frequent responses to glycoproteins and tegument and immediate-early (IE) proteins of HSV-2, T c

27 fected ECs inefficiently acquired the virion tegument and secondary envelope.

28 of connecting capsid and membrane across the tegument and that the ability to switch between monomeri

29 urring via numerous interactions between the tegument and the capsid, within the tegument, and betwee

30 capsid, within the tegument, and between the tegument and the envelope.

31 a process that is normally overcome by viral tegument and/or immediate-early proteins.

32 ween the tegument and the capsid, within the tegument, and between the tegument and the envelope.

33 ich were previously localized to the capsid, tegument, and envelope layers using traditional biochemi

34 structure consisting of a DNA-filled capsid, tegument, and envelope.

35 in 13/14 (VP13/14), also known as UL47, is a tegument antigen targeted by CD8(+) T cells from HSV-ser

36 to extend across the entire thickness of the tegument (approximately 50 nm).

37 y could serve as organizing features for the tegument, as they have the potential to reach all parts

38 our observations suggest that while complete tegument assembly may not be necessary for VZV replicati

39 The EBV tegument BNRF1 is a DAXX-interacting protein required fo

40 ccurs in the cytoplasm, where the capsid and tegument bud into host cell membranes.

41 is hypothesized to facilitate the budding of tegumented capsid into glycoprotein-embedded membrane du

42 protein, pUL36, which occupies the layer of tegument closest to the capsid, is essential for formati

43 recruiting cell-regulating factors into the tegument compartment as 'cargoes', and should inform fut

44 tures of HHV-6B capsid and capsid-associated tegument complex (CATC) obtained by cryoEM and sub-parti

45 cterization of a multicomponent glycoprotein-tegument complex found in herpes simplex virus 1 (HSV-1)

46 tructions confirm that the capsid associated tegument complex is present on capsids prior to nuclear

47 terfaces involving SCP and capsid-associated tegument complexes (CATC): SCPs crown pentons/hexons and

48 an icosahedral capsid with capsid-associated tegument complexes (CATCs) and facilitates translocation

49 A-translocating portal and capsid-associated tegument complexes from cryogenic electron microscopy im

50 The alphaherpesvirus UL51 protein is a tegument component that interacts with the viral glycopr

51 wn function, UL88, in maintaining the proper tegument composition of HCMV virions.

52 The tegument comprises at least 20 proteins destined for del

53 nvelope layer, which encloses the capsid and tegument, contains viral transmembrane proteins anchored

54 respectively) in the KSHV capsid-associated tegument cryo-EM structure.

55 In all latency models tested, tegument-delivered pp71 was found to be colocalized with

56 Lytic infection is not initiated because the tegument-delivered transactivator protein pp71 fails to

57 e organized in a gammaherpesvirus, with five tegument densities capping each penton vertex, a pattern

58 Each KSHV tegument density can be divided into three prominent reg

59 roblast cells highlight virion uncoating and tegument disassembly as a divergence point between produ

60 Further definition of tegument disassembly may permit the development of inter

61 As the tegument does not have a regular structure, the question

62 Viral tegument, envelope, and some nonstructural proteins loca

63 The disruption in tegument formation from ORF52 suppression, therefore, pr

64 xport by budding into the perinuclear space, tegument formation, and envelopment to complete de novo

65 (DNA polymerase auxiliary subunit) and UL46 (tegument) had no measurable influence, while two indepen

66 ts, the icosahedral capsid and the amorphous tegument, has been extensively studied, but the identity

67 specially with respect to acquisition of the tegument; however, it is thought to involve the stepwise

68 The function of tegument ICP0 is unknown.

69 some the blocked entry of virions containing tegument ICP0, including ICP0 mutants that are defective

70 ut the protein is also packaged in the viral tegument, indicating that BPLF1 may function both early

71 To understand capsid assembly and capsid-tegument interactions, here we report atomic structures

72 36 stabilizes other components of the vertex-tegument interface.

73 ity reaches the greatest level at the capsid-tegument interfaces involving SCP and capsid-associated

74 f assembly, during which the majority of the tegument is acquired and final envelopment occurs.

75 The herpes simplex virus 1 (HSV-1) tegument is known to contact the capsid at its vertices,

76 irus, the interaction between the capsid and tegument is limited to the capsid vertices and involves

77 Assembly of the herpesvirus tegument is poorly understood but is believed to involve

78 nal is sent from the virion surface into the tegument is unknown.

79 d is, in turn, surrounded by a proteinaceous tegument layer and a lipid envelope.

80 ent protein were of smaller size because the tegument layer between capsid and viral envelope was red

81 The tegument layer of herpesviruses comprises a collection o

82 ntry, since ICP0 is a component of the inner tegument layer of the virion.

83 that the ORF7 protein is a component of the tegument layer of VZV virions.

84 nner rim of the upper hexon channel into the tegument layer.

85 viral proteins UL47 and UL48 into the virion tegument layer.IMPORTANCE A better understanding of the

86 ut fail to fully uncoat or disassemble their tegument layers, leading to the establishment of latency

87 ant enveloped particles with thicker, oblong tegument layers.

88 nalysis shows a significant reduction in the tegument levels of pp71, UL47, and UL48 in viruses lacki

89 sviruses, contain a protein layer termed the tegument localized between the capsid and the envelope.

90 It is well known that proteins in the tegument (located between the viral capsid and envelope

91 resides in the innermost layer of the viral tegument, lying between the capsid and the envelope.

92 aptive evolutions in homeostasis regulation, tegument maintenance and lipid uptakes, and differential

93 n fluorescent protein (GFP) and contains the tegument multifunctional ORF45 protein as a fusion prote

94 tracellular particles, having neither a full tegument nor an envelope.

95 Confocal studies on sections of tegument, nucellus, endosperm, and embryo showed that th

96 ression profiles for muscle, nervous system, tegument, oesophageal gland, parenchymal/primordial gut

97 The tegument of all herpesviruses contains a capsid-bound la

98 The tegument of all herpesviruses contains a high-molecular-

99 UL21 is a conserved protein in the tegument of alphaherpesviruses and has multiple importan

100 The tegument of herpesviruses is a highly complex structural

101 und prominently in the dorsal surface of the tegument of males.

102 sid proteins, suggesting its presence in the tegument of the HSV-1 virion.

103 found that VZV ORF12 protein, located in the tegument of virions, enhances AP-1 reporter activity.

104 k in maturation limbo, unable to acquire the tegument or outer (envelope) layers.

105 The tegument, or middle layer, of herpesviruses comprises bo

106 verall, the results indicate that stochastic tegument packaging provides a mechanism enabling probabi

107 cytomegalovirus (HCMV) high-molecular-weight tegument protein (HMWP, pUL48; 253 kDa) and the HMWP-bin

108 that several epitopes from the HSV-1 virion tegument protein (VP11/12) encoded by UL46 are targeted

109 There results indicate that ORF52 is a tegument protein abundantly present in extracellular vir

110 , we show that the viral protein 1/2 (VP1/2) tegument protein associates with the dynein/dynactin mic

111 We reported previously that EBV tegument protein BGLF2 activates p38 and enhances EBV re

112 In summary, the EBV tegument protein BGLF2, which is delivered to the cell a

113 CATC): SCPs crown pentons/hexons and mediate tegument protein binding, and CATCs bind and rotate all

114 ound that the Epstein-Barr virus (EBV) BGLF2 tegument protein binds to a protein in the type I interf

115 Here, we demonstrate that the EBV tegument protein BNRF1 binds the histone H3.3 chaperone

116 We show that the EBV tegument protein BNRF1 functions to regulate chromatin a

117 We propose that EBV tegument protein BNRF1 replaces ATRX to reprogram Daxx-m

118 We report that EBV tegument protein BNRF1, discovered by other investigator

119 A herpes simplex virus tegument protein brought into the cell during infection

120 uced by an immediate early (IE) gene-encoded tegument protein called ORF45, to promote the late trans

121 This may be a result of altered virion tegument protein composition, as Western blot analysis s

122 Thus, a tegument protein delivered to cells during virus infecti

123 We find that HCMV virion binding and tegument protein delivery are insufficient for HCMV-medi

124 For example, the pp71 tegument protein encoded by the UL82 gene of human cytom

125 xpression and localization of MDV pUL47-EGFP tegument protein is potentially important for the unique

126 on and extends our understanding of the HCMV tegument protein network that is required to interface t

127 The UL16 tegument protein of herpes simplex virus (HSV) is conser

128 UL16 is a tegument protein of herpes simplex virus (HSV) that is c

129 The UL16 tegument protein of herpes simplex virus 1 (HSV-1) is co

130 The UL11 tegument protein of herpes simplex virus plays a critica

131 sly shown that ORF45, an immediate-early and tegument protein of Kaposi's sarcoma-associated herpesvi

132 The ORF75c tegument protein of murine gammaherpesvirus 68 (MHV68) p

133 se (DUB) activity, embedded within the large tegument protein open reading frame (ORF)64, gained the

134 , we show that the gammaherpesvirus-specific tegument protein ORF52 is critical for maturation of RRV

135 The HCMV virion tegument protein pp150 (ppUL32) is an essential protein

136 ) of infected cells co-localizing with virus tegument protein pp150 and the formation of vAC was comp

137 The capsid-associated tegument protein pp150 is released from maturing endosom

138 P, Tri1, Tri2, and SCP and the HCMV-specific tegument protein pp150-totaling 4000 molecules and 62 di

139 seen in the absence of the immunomodulatory tegument protein pp65 (pUL83).

140 s (HCMV) immediate early protein IE1 and the tegument protein pp71 are required for efficient infecti

141 E protein IE1 targets PML and Sp100, and its tegument protein pp71 targets hDaxx and ATRX.

142 Tegument protein pp71 was cytoplasmic, and immediate-ear

143 d the conserved, multifunctional HCMV virion tegument protein pUL103 as important for cVAC biogenesis

144 We previously showed that the HCMV tegument protein pUL103 is required for cVAC biogenesis.

145 oteins, pUL17 and pUL25, and the large inner tegument protein pUL36.

146 capsid protein pUL25 and the capsid-tethered tegument protein pUL36.

147 cruited to HSV-1 capsids by the capsid-bound tegument protein pUL37 to promote efficient cytoplasmic

148 cted interaction between pUS10 and the inner tegument protein pUL37, which binds cytosolic capsids, c

149 cent protein (EGFP) to the C terminus of the tegument protein pUL47 (vUL47-EGFP) or pUL49 (vUL49-EGFP

150 The HCMV tegument protein pUL83 inhibits this response by interac

151 gammaherpesvirus 68 (MHV68), which encodes a tegument protein that induces PML degradation.

152 ific mAb for VZV ORF9, a membrane-associated tegument protein that interacts with glycoprotein E (gE)

153 Human cytomegalovirus UL103 encodes a tegument protein that is conserved across herpesvirus su

154 We demonstrate here that UL94 is a tegument protein that is expressed with true-late kineti

155 herpesvirus (KSHV) is an immediate-early and tegument protein that plays critical roles in antagonizi

156 pp150 is a capsid-proximal tegument protein that preserves the integrity of nucleoc

157 ated herpesvirus, ORF52 is a highly abundant tegument protein tightly associated with the capsid.

158 elopment and that it interacts with the UL37 tegument protein to facilitate cytoplasmic virion envelo

159 The HSV-1-encoded tegument protein UL16 is involved in multiple events of

160 We report that tegument protein UL16 is unstable, i.e., rapidly degrade

161 Inner tegument protein UL37 is conserved among all three subfa

162 detected by the colocalization of the virion tegument protein UL37, with dynein required for loading

163 s gD and gB and the novel T cell antigen and tegument protein UL40, and we compared this vaccine to a

164 We found that (i) HSV-1 tegument protein UL46 interacts with and colocalizes wit

165 ne of the least-studied proteins of HSV, the tegument protein UL46, and that function involves the ev

166 The human cytomegalovirus tegument protein UL69 has been shown to be required for

167 known about the human cytomegalovirus (HCMV) tegument protein UL88.

168 rated that UL99 interacts with the essential tegument protein UL94 in infected cells as well as in th

169 ic assembly complex shows that the essential tegument protein UL99 (pp28) exhibits aberrant localizat

170 The HCMV tegument protein UL99 (pp28) is essential for viral repl

171 ionally, a deletion of the gene encoding the tegument protein Vhs ablated most of the depletion of AT

172 The herpes simplex virus (HSV) tegument protein VP1-2 contains an N-terminal nuclear lo

173 munodominant epitopes derived from the HSV-1 tegument protein VP11/12.

174 fic to three epitopes derived from the HSV-1 tegument protein VP13/14 (VP13/14286-294,VP13/14504-512,

175 nity and UL19 (capsid protein VP5) and UL47 (tegument protein VP13/14) as T cell immunogens.

176 munogens UL19 (capsid protein VP5) and UL47 (tegument protein VP13/14) would enhance the protection p

177 ents and fluorescence detection of the HSV-1 tegument protein VP16 were used to analyze the mechanism

178 teins, the envelope glycoprotein D (gD), the tegument protein VP22 (encoded by the UL49 gene), and ri

179 We demonstrate that the tegument protein VP22 bridges a complex between glycopro

180 However, levels of the tegument protein VP22 were also dramatically reduced in

181 while expression of the nonglycosylated IE62 tegument protein was unchanged.

182 Virions lacking the M25 tegument protein were of smaller size because the tegume

183 virus 8 replication and uptake of the ORF45 tegument protein were tested in human retinal pigment ep

184 These results indicate that a viral tegument protein which is delivered to cells upon infect

185 IG-I)-deficient cells and that KSHV ORF64, a tegument protein with deubiqutinase (DUB) activity, supp

186 virus 1 (HSV-1) U(L)21 gene encodes a 62-kDa tegument protein with homologs in the alpha-, beta-, and

187 to regulate not just the interaction of this tegument protein with its viral binding partners but als

188 This study identifies a role for a viral tegument protein with unknown function, UL88, in maintai

189 s report, we further characterize ORF52 as a tegument protein with vital roles during KSHV lytic repl

190 UL23 (thymidine kinase), UL25 (DNA packaging tegument protein), and UL52 (helicase-primase primase su

191 H), ORF25 (major capsid protein) and ORF64 (tegument protein).

192 e most frequently recognized L Ag, the BNRF1 tegument protein, also recognized latently infected, gro

193 e 52 (ORF52), a multifunctional and abundant tegument protein, as being the only virally encoded comp

194 We identified a viral tegument protein, BGLF2, which activates members of the

195 As might be expected for a tegument protein, interactions were identified that sugg

196 Through its interactions with pUL47, another tegument protein, it spares from degradation viral mRNAs

197 A capsid-bound tegument protein, pUL37, is an essential effector of ret

198 ons in the genes for pUL36 and another inner tegument protein, pUL37, to analyze the contributions of

199 on an intact microtubule network and a viral tegument protein, pUL51.

200 lex in infected cells with another conserved tegument protein, pUL7.

201 ORF52, an abundant gammaherpesvirus-specific tegument protein, subverts cytosolic DNA sensing by dire

202 ucture of the N-terminal half of a conserved tegument protein, UL37, from HSV-1.

203 Like the capsid-associated pp150 tegument protein, we initially detected capsid proteins

204 re--the first for any alphaherpesvirus inner tegument protein--reveals an elongated molecule of a com

205 During primary infection, tegument protein-specific B cells expressed an activated

206 h and similar frequencies of gB-specific and tegument protein-specific B cells following primary HCMV

207 equency and the phenotype of gB-specific and tegument protein-specific B cells were studied in a coho

208 showed that virion-to-virion levels of pp71 tegument protein-the major viral transactivator protein-

209 ely due to its roles in virion assembly as a tegument protein.

210 a gammaherpesvirus-specific immediate-early tegument protein.

211 ding partner of the membrane-associated UL11 tegument protein.

212 hat a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein.

213 ivity of the viral virion host shutoff (vhs) tegument protein.

214 In neurons transfected with tegument proteins and gE/gI or US9, there was good evide

215 ore, genes encoding latent, early lytic, and tegument proteins and glycoproteins were found to contai

216 lieved to involve interactions between outer tegument proteins and the cytoplasmic domains of envelop

217 prehensive investigation of the functions of tegument proteins and their roles in viral replication m

218 protein interactions involving virus-encoded tegument proteins are critical for virus assembly and ar

219 However, several tegument proteins are known to be essential for proper p

220 s, for the first time, how capsid-associated tegument proteins are organized in a gammaherpesvirus, w

221 As no other tegument proteins are retained in significant amounts, i

222 Certain tegument proteins can also regulate viral processes.

223 tein interactions and less stably associated tegument proteins compared with other human herpesviruse

224 , little is known about the function of many tegument proteins during virus replication.

225 The tegument proteins encoded by ORF11 and ORF9 of varicella

226 Whether and how such tegument proteins exist in gammaherpesviruses have been

227 tly relates to a matrix of several different tegument proteins formed in infected cells that bind to

228 Here we focus on two tegument proteins from herpes simplex virus 1 (HSV-1), p

229 Our study focuses on two tegument proteins from herpes simplex virus 1 that are c

230 suggest that gE/gI and US9 binding to these tegument proteins has neuron-specific effects on virus H

231 However, no such tegument proteins have been identified for gammaherpesvi

232 Capsid-associated tegument proteins have been identified in alpha- and bet

233 Several capsid-associated tegument proteins have been identified in the alpha- and

234 Tegument proteins have important roles in assembling vir

235 Systematic exploration of the roles of tegument proteins in capsid trafficking requires detaile

236 r understanding of the role and functions of tegument proteins in HCMV, many of which remain uncharac

237 the U(L)49 gene, is one of the most abundant tegument proteins in HSV-1 virions.

238 mensional structure of the capsid-associated tegument proteins in the prototypical member of gammaher

239 The incorporation of tegument proteins into the herpes simplex virus 1 (HSV-1

240 support the notion that the incorporation of tegument proteins into the herpesviruses is a very compl

241 eins required for cellular entry, as well as tegument proteins involved in regulating lytic replicati

242 ral mutants, which showed that each of these tegument proteins is critical for processing, transport,

243 t the organization of KSHV capsid-associated tegument proteins is highly similar to that in alphaherp

244 gG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and

245 first time that these conserved herpesvirus tegument proteins localize to focal adhesions in additio

246 The tegument proteins of herpesviruses, including Kaposi's s

247 UL46 is one of the most abundant tegument proteins of HSV-1, but a well-established funct

248 Transient expression of the viral tegument proteins of pp71 and UL26 reduced NICD1 and Jag

249 may provide flexibility in interacting with tegument proteins or the DNA-packaging machinery at the

250 posi's sarcoma-associated herpesvirus (KSHV) tegument proteins ORF33 and ORF45 is crucial for progeny

251 Tegument proteins play both structural and regulatory fu

252 These tegument proteins play essential roles in viral propagat

253 Viral tegument proteins play important roles in maintaining th

254 Tegument proteins pp150 and pUL96 function at a late ste

255 pesvirus of chickens, we analyze the role of tegument proteins pUL47 and pUL48 in the whole life cycl

256 intense investigation, the function of many tegument proteins remains unknown.

257 ail, virion packaging of UL11, but not other tegument proteins such as VP22 and VP16, was reduced by

258 owever, there were lower quantities of these tegument proteins that coprecipitated with gE/gI and US9

259 mechanism of reactivation in the absence of tegument proteins that enable gene expression in product

260 ractions between viral membrane proteins and tegument proteins that encrust capsids.

261 systematically evaluated the ability of KSHV tegument proteins to modulate the activation of an integ

262 s function of gE requires the cooperation of tegument proteins UL11, UL16, and UL21.

263 Tegument proteins UL16 and UL11 of herpes simplex virus

264 The tegument proteins UL36 and UL37 are known to be transpor

265 the direct interaction between two essential tegument proteins VP1/2 and VP16 is required for connect

266 major HSV-1 capsid protein VP5; and the HSV tegument proteins VP11/12 (pU(L)46) and VP13/14 (pU(L)47

267 d analysis of the radial distribution of the tegument proteins VP16, VP1/2 and pUL37, and envelope pr

268 All four of these tegument proteins were also pulled down with US9.

269 In addition, several tegument proteins were found in high-molecular-weight co

270 ruses (alpha, beta, and gamma) encode unique tegument proteins with specialized functions.

271 mpared with production of antibodies against tegument proteins, and this likely reduces the control o

272 iminate envelope-anchored glycoproteins from tegument proteins, both in purified virions and in virio

273 HHV-6B capsid assembly and the roles of its tegument proteins, including not only the beta-herpesvir

274 s and migrates to the nucleus, whereas other tegument proteins, including pp71, remain endosome assoc

275 providing a flexible scaffold to which other tegument proteins, including UL37, bind.

276 ted neurons showed that UL16 and three other tegument proteins, namely, VP22, UL11, and UL21, bound e

277 new protein-protein interactions among HCMV tegument proteins, some of which are likely important fo

278 ding genes encoding latent, early lytic, and tegument proteins, such as substitutions within transmem

279 the incorporation of ORF45, as well as other tegument proteins, suggesting that ORF52 is important fo

280 In cells transfected with individual tegument proteins, this matrix is less prevalent.

281 implex virus 1 UL11 and cytomegalovirus UL99 tegument proteins, which are involved in the final envel

282 e viruses is controlled by capsid-associated tegument proteins, yet their specific roles have not yet

283 y shrouding the pentons that we attribute to tegument proteins.

284 t recombinantly expressed HSV-2 envelope and tegument proteins.

285 recombinantly expressed herpes envelope and tegument proteins.

286 rpesviruses, two capsid-associated, or inner tegument, proteins, UL37 and UL36, control cytosolic tra

287 -TSP-3) that are abundantly expressed in the tegument proteome of O. viverrini.

288 nst recurrent genital herpes and promote the tegument RR2 protein as a viable candidate Ag to be inco

289 he UL16-gE interaction may play roles in the tegument signaling mechanism, virus budding, and the gE-

290 ern of immunogold-labeled SmNPP-5 within the tegument; some immunogold particles are scattered throug

291 Despite the high level of tegument structural similarities between KSHV and alphah

292 n from icosahedral symmetry, KSHV portal and tegument structures have largely been obscured in previo

293 tudies confirm the importance of CD63 family tegument tetraspanins in parasitic flukes and support ef

294 nstrated that, compared to capsids lacking a tegument, these capsids (called T36 capsids) had tufts o

295 flukes resulted in phenotypes with increased tegument thickness, increased vacuolation (tsp-2) and re

296 e it establishes latency in part because its tegument-transactivating protein, pp71, remains associat

297 stone deacetylases (HDACs) because pp71, the tegument transactivator that travels to the nucleus and

298 This super-Poissonian tegument variability promoted alternate replicative stra

299 The HSV type 1 tegument virion phosphoprotein (VP) 11/12 (VP11/12) is a

300 nd VP16 is required for connecting the inner tegument with the outer tegument.