ライフサイエンスコーパス: terminase

1 equivalents of L-terminase (1S-terminase:2L-terminase).

2 ar helicases and viral DNA packaging motors (terminases).

3 tor rather than the nuclease activity of the terminase.

4 ase activity to a level comparable with FL-L-terminase.

5 rminal domain for interacting with the large terminase.

6 onucleolytic activity in the context of FL-L-terminase.

7 nus and at 5.7 nm from the C terminus of the terminase.

8 al domains required for interaction with the terminase.

9 fic terminase and reduce packaging by lambda-terminase.

10 ocess that requires ATP hydrolysis by lambda terminase.

11 ty shell, prohead, and the packaging enzyme, terminase.

12 he concatemer-cutting endonuclease reside in terminase.

13 )28-pU(L)33 complex to form the likely viral terminase.

14 ical for structure and function of the small terminase.

15 odimeric viral DNA packaging protein, called terminase.

16 med capsids through the actions of the viral terminase.

17 inase and human cytomegalovirus (HCMV) small terminase.

18 binds specifically to the ATPase domain of L-terminase.

19 nd between GFP portal and single dye-labeled terminases.

20 ved from conserved nuclease domains in phage terminases.

21 ic S-terminase bound to two equivalents of L-terminase (1S-terminase:2L-terminase).

22 bound to two equivalents of L-terminase (1S-terminase:2L-terminase).

23 A translocation is accomplished by the phage terminase, a powerful molecular motor consisting of larg

24 Genome maturation is carried out by the terminase, a protein complex that mediates both insertio

25 sence or absence of genes coding for a phage terminase (ACICU_02185), a sialic acid synthase (ACICU_0

26 We further show that lambda terminase adopts a heterogeneous mixture of higher-order

27 Genome maturation is carried out by the terminase, an enzyme complex that mediates both the inse

28 fied in herpes simplex virus 1 (HSV-1) large terminase and human cytomegalovirus (HCMV) small termina

29 r the specific endonuclease activity of HK97 terminase and is essential for phage head morphogenesis.

30 atalytic carboxylate in bacteriophage lambda terminase and probe its mechanistic role.

31 cause enhanced DNA packaging by 21-specific terminase and reduce packaging by lambda-terminase.

32 The Xfas53 terminase and structural genes are related at a protein

33 reported, revealing the coupling between the terminase and the connector forming a continuous channel

34 ive site, which are conserved in herpesvirus terminases and bear great similarity to the phage T4 gp1

35 The packaging machine consists of a "small terminase" and a "large terminase" component.

36 d (gp23), portal (gp20), motor (gp17, large "terminase"), and regulator (gp16, small terminase), lead

37 resolution provides the first glimpse of P22 terminase architecture and implies two distinct modes of

38 pUL6 and the associated viral terminase are required for processing of concatemeric vi

39 tively target specific DNA, and no GTA small terminases are known.

40 The structure of the terminase assembled into the complex showed a different

41 e tightly coupled reactions are catalyzed by terminase assembled into two functionally distinct nucle

42 ite with which a molecular motor, termed the terminase, associates during the DNA packaging reaction.

43 dentify a portal protein domain critical for terminase association with the capsid and suggest that b

44 and C-terminal nuclease domain, suggest that terminase association with the prohead portal and commun

45 inase morphological change, we generated the terminase atomic model based on the crystallographic str

46 ng studies revealed that the N-terminus of L-terminase ATPase domain (residues 1-58) contains a minim

47 l HTH motifs of the Bacillus phage SF6 small terminase bind the packaging regions of SF6 and related

48 domain (residues 1-58) contains a minimal S-terminase binding domain sufficient for stoichiometric a

49 with residues 140-162 of S-terminase, the L-terminase binding domain.

50 eaving a binary complex consisting of lambda terminase bound to the head end of the adjacent genome.

51 predominantly of one copy of the nonameric S-terminase bound to two equivalents of L-terminase (1S-te

52 leavage and packaging functions of the viral terminase, but not for terminase complex assembly.

53 ave determined the structure of the T7 large terminase by electron microscopy.

54 own a decrease in distance from the phage T4 terminase C terminus to portal consistent with a linear

55 Mutations in the phage T4 terminase C-motif lead to loss of stimulated ATPase and

56 This supports a model in which ES18 terminase can move substantial distances along the DNA b

57 ifferent functional classes of phage-encoded terminases can usually be predicted from their amino aci

58 taining two 21-bp direct repeats and a major terminase cleavage site in the phage genome.

59 Thus we postulate that viral terminases cleave DNA by the canonical RuvC-like mechani

60 rst direct visualization of a purified viral terminase complex analyzed in the absence of DNA and pro

61 gp1 in formation of the packaging-competent terminase complex and assembly of the terminase with the

62 genome involve an ordered interaction of the terminase complex and pUL25 with pUL17 at the portal ver

63 unctions of the viral terminase, but not for terminase complex assembly.

64 The human cytomegalovirus terminase complex cleaves concatemeric genomic DNA into

65 The human cytomegalovirus (HCMV) terminase complex consists of several components acting

66 mass spectrometry revealed that the purified terminase complex consists predominantly of one copy of

67 ication, indicating that drugs targeting the terminase complex could be safe and selective.

68 We propose that the GTA terminase complex could become a streamlined model syste

69 pUL6 are required for capsid binding of the terminase complex in the nucleus, (iii) pUL17 is importa

70 Letermovir, a new viral terminase complex inhibitor, has been approved for the p

71 late time point, similarly to other reported terminase complex inhibitors.

72 The structure of the complete connector-terminase complex is also reported, revealing the coupli

73 The herpesviral terminase complex is part of the intricate machinery tha

74 The tripartite terminase complex of herpesviruses assembles in the cyto

75 ds milligram quantities of the S-terminase:L-terminase complex of the Salmonella phage P22.

76 In vivo assembled terminase complex was affinity-purified and stabilized b

77 pUL17 as key viral factors for engaging the terminase complex with the capsid and the subsequent cle

78 One component of the human cytomegalovirus terminase complex, pUL89, provides the endonucleolytic a

79 mpered by the inability to purify the intact terminase complex.

80 known about the architecture of an assembled terminase complex.

81 aracteristics of proteins that make up viral terminase complexes to identify or design additional ter

82 In this study, terminase complexes were isolated by tandem-affinity pur

83 n of codons 400 to 420 of U(L)15, encoding a terminase component, was analyzed.

84 consists of a "small terminase" and a "large terminase" component.

85 The varicella-zoster virus (VZV) terminase components (pORF25, pORF30, and pORF45/42) hav

86 conclude that interactions between putative terminase components are tightly linked to successful vi

87 portal protein encoded by UL6, the putative terminase components encoded by UL15, UL 28, and UL33, t

88 Whether putative terminase components localized in the nucleus or cytopla

89 kaging other than its interaction with other terminase components.

90 Terminases comprise essential components of molecular mo

91 The existence of two terminase conformations and its possible relation to the

92 The docking of the threaded model in both terminase conformations showed that the transition betwe

93 Terminases consisting of two to three packaging-related

94 The terminase contains a large subunit that is thought to cl

95 defining the characteristics of a "GTA-type" terminase could be an important step toward novel GTA id

96 show that either ATP or ADP is required for terminase cutting at cos, to generate the active, DNA pa

97 ing on "endless" concatemeric DNA in vivo by terminase depends upon interaction with the DNA loaded g

98 th respect to the assembly and function of a terminase DNA-packaging motor are discussed.

99 veals a common scheme for oligomerization of terminase DNA-recognition components, and provides insig

100 The finding that the portal and terminase do eventually interact was supported by the ob

101 ion motors consist of a multimeric packaging terminase docked onto a unique procapsid vertex containi

102 The terminase docks onto the portal protein complex embedded

103 establish the function for each of the small terminase domains.

104 scuss the model in relation to proposals for terminase-driven DNA translocation in other phages.

105 A novel terminase-driven mechanism is proposed for translocation

106 Many dsDNA viruses encode DNA-packaging terminases, each containing a nuclease domain that resol

107 revealed that numerous phage and viral large terminases encode a common Walker-B motif in the N-termi

108 l vertex, and all components of a tripartite terminase enzyme are required to both cleave and package

109 Terminase enzyme complexes, which facilitate ATP-driven

110 bda genome into the viral capsid, the lambda terminase enzyme introduces symmetric nicks, 12 bp apart

111 As the terminase enzyme packages the genome into the phage caps

112 In addition to the terminase enzyme, the packaging chaperone, FI protein (g

113 and herpes viruses require the activity of a terminase enzyme, which is comprised of large and small

114 DNA is packaged into a procapsid shell by a terminase enzyme.

115 preformed procapsid structure, catalyzed by terminase enzymes and fueled by ATP hydrolysis.

116 Terminase enzymes are common to both prokaryotic and euk

117 Terminase enzymes are common to double-stranded DNA (dsD

118 Terminase enzymes are viral motors that package DNA into

119 enomes linked in a head-to-tail fashion, and terminase enzymes perform two essential functions: 1) ex

120 Terminase enzymes processively excise and package monome

121 Viral terminase enzymes serve as genome packaging motors in ma

122 This work is thus of relevance to all terminase enzymes, both prokaryotic and eukaryotic.

123 conservation of these structural elements in terminase enzymes, this mechanism may be universal for v

124 ntains the nuclease and ATPase activities of terminase, exists as a stable monomer with an alpha/beta

125 In contrast, small terminase exposes a classical NLS at the far C terminus

126 ined by in-drop proteolysis of full-length L-terminase (FL-L-terminase) reveals a central seven-stran

127 a monopartite NLS at the N terminus of large terminase, flanking the ATPase domain, that is conserved

128 support a model in which MltG functions as a terminase for both classes of PG synthases by cleaving P

129 Escherichia coli MltG (YceG) as a potential terminase for glycan polymerization that is broadly cons

130 ycosylase MltG was identified as a potential terminase for PG synthesis in Escherichia coli.

131 We find that purified, recombinant lambda terminase forms a homogeneous, heterotrimeric structure,

132 The crystal structure of the large terminase from the Geobacillus stearothermophilus bacter

133 Here, we identified the small terminase from the model Rhodobacter capsulatus GTA, whi

134 different oligomerization states for a small terminase from the T4 family of phages.

135 sis reveals that the role of HNH proteins in terminase function is widespread among long-tailed phage

136 y conserved region (region IX) essential for terminase function.

137 uence despite the selective pressure to keep terminase gene products active and localized in the nucl

138 e (HNHE) next to their cohesive end site and terminase genes.

139 cularly permuted genome that begins with the terminase genes.

140 y a variety of factors; Mg, NaCl, ATP, small terminase gp16 and N-terminal ATPase domain.

141 nase motor (gp17), and 11- or 12-meric small terminase (gp16).

142 nuclease activity is modulated by the 'small terminase', gp16, by the N-terminal ATPase domain of gp1

143 An 18-kDa small terminase, gp16, is also essential, but its role in DNA

144 packaging assay employed purified proheads, terminase (gp17 + gp16), and ATP to encapsidate DNA resi

145 hydrolysis in the pentameric phage T4 large "terminase" (gp17) motor.

146 , present in the C-terminal domain of 'large terminase' gp17, has been defined by mutational, biochem

147 h resides in the C-terminal domain of large 'terminase' gp17, is a weak endonuclease and regulated by

148 e phage T4 motor, a pentamer of 70-kDa large terminase, gp17, is the fastest and most powerful motor

149 we show that residue K428 of a bacteriophage terminase gp2 nuclease domain mediates binding of the me

150 ucture and regulation of bacteriophage P22 L-terminase (gp2).

151 While the enzymology of lambda-terminase has been well described, the nature of the cat

152 packaging experiments show that these mutant terminases have lost the ability to discriminate between

153 eneral interest, given the parallels between terminases, helicases, and other motor proteins.

154 We report that the ATPase motors of terminases, helicases, translocating restriction enzymes

155 Bacteriophage lambda terminase holoenzyme is a hetero-oligomer composed of th

156 results with respect to the two roles of the terminase holoenzyme, DNA maturation and DNA packaging,

157 nal on viral DNA and directs assembly of the terminase holoenzyme.

158 finger domain found in all herpesvirus ORF30 terminase homologs but also identified a novel, highly c

159 We examined the role of the small terminase in GTA production and propose a structural bas

160 Our data allowed the prediction of small terminases in diverse GTA producer species, and defining

161 ease function of human cytomegalovirus (HCMV terminase) in vitro.

162 wne-infected cultures treated with the viral terminase inhibitor 2-bromo-5,6-dichloro-1-beta-d-ribofu

163 ch that combines treatment with an antiviral terminase inhibitor and purification by a simplified pro

164 egalovirus (CMV) deoxyribonucleic acid (DNA) terminase inhibitor, was recently approved for prophylax

165 rus drugs include viral helicase-primase and terminase inhibitors.

166 The terminase is a powerful motor that converts ATP hydrolys

167 Herpes simplex virus (HSV) terminase is an essential component of the molecular mot

168 The HSV terminase is believed to consist of the UL15, UL28, and

169 During translocation, terminase is docked on the prohead's portal protein.

170 aging of the viral genome, whereas the large terminase is responsible for the ATP-powered translocati

171 One of the functions of the small terminase is to initiate packaging of the viral genome,

172 Phage lambda with the chimeric terminase is unable to form plaques, but pseudorevertant

173 terminase (S-terminase) subunit and a large terminase (L-terminase) subunit, transiently docked at t

174 ocessing nuclease, both located in the large terminase (L-terminase) subunit.

175 em that yields milligram quantities of the S-terminase:L-terminase complex of the Salmonella phage P2

176 In addition, using active terminases labeled at the N- and C-terminal ends with a

177 We propose a scheme for the roles of P22 terminase large and small subunits in the recruitment an

178 of ectopically expressed and highly purified terminase large and small subunits.

179 ate for interaction with and cleavage by the terminase large subunit prior to DNA translocation into

180 the similarity of major capsid proteins and terminase large subunits further suggests they form a di

181 ay structure of the C-terminal domain of the terminase large-subunit pUL15 (pUL15C) from HSV-1.

182 rge "terminase"), and regulator (gp16, small terminase), leading to precise orchestration of the pack

183 In HSV, the terminase likely comprises the U(L)15, U(L)28, and U(L)3

184 We show that the activity of gp74 in terminase-mediated cleavage of the phage cos site relies

185 To understand in molecular terms the terminase morphological change, we generated the termina

186 dodecameric portal (gp20), pentameric large terminase motor (gp17), and 11- or 12-meric small termin

187 Here, we present mechanistic studies on the terminase motor from bacteriophage lambda.

188 Many viruses use a powerful terminase motor to pump their genome inside an empty pro

189 face of the expanded capsid lattice, and the terminase motors tightly package DNA, generating up to a

190 cribed in this study represent the first VZV terminase mutants reported to date.

191 ntercalating dyes arrest packaging, but rare terminase mutations confer resistance.

192 nt herpesviruses to regulate the kinetics of terminase nuclear import, reflecting a mechanism of viru

193 Crystal structures of the large terminase nuclease from the thermophilic bacteriophage G

194 Terminase nucleases resemble the RNase H-superfamily nuc

195 The terminase of herpes simplex virus (HSV) is composed of t

196 zed the interactions between small and large terminases of T4-related phages.

197 The mechanism by which a ring-shaped small terminase oligomer binds viral DNA has not previously be

198 Packaging with a C-terminal fluorescent terminase on a GFP portal prohead, FRET shows a reductio

199 Bacteriophage terminases package DNA through the portal ring of a proc

200 etent for high affinity binding to the large terminase packaging protein, and a symmetric ring in the

201 conformation when compared with the isolated terminase pentamer.

202 residue, and that the large subunit of HK97 terminase physically interacts with gp74.

203 Viral terminases play essential roles as components of molecul

204 sembly and DNA packaging genes, which encode terminase, portal, scaffold, and coat proteins, are extr

205 Remarkably, terminases, portal proteins, and shells of tailed bacter

206 s-driven conformational changes of portal or terminase powering DNA motion.

207 substrate did not remain stably bound by the terminase-prohead.

208 teriophage T4 motor, a pentamer of the large terminase protein (gp17) assembled at the portal vertex

209 rmed between the empty prohead and the large terminase protein (gp17) that can capture and begin pack

210 g of only two components, proheads and large terminase protein (gp17; 70 kDa), is constructed.

211 The crystal structure of a full-length small terminase protein from the Siphoviridae bacteriophage SF

212 The small terminase protein is essential for the initial recogniti

213 Most bacteriophages use the small terminase protein to identify their own genome and direc

214 n phage T4, there is evidence that the large terminase protein, gene product 17 (gp17), assembles int

215 The small terminase protein, gp16 (18 kDa), is not only not requir

216 The small terminase protein, gp16, inhibited DNA binding, which wa

217 l terminase protein, gpl6 (18kDa), the large terminase protein, gp17 (70kDa), and the dodecameric por

218 The phage T4 motor is composed of the small terminase protein, gpl6 (18kDa), the large terminase pro

219 An essential part of this machine, the large terminase protein, processes viral DNA into constituent

220 Biochemical studies of the terminase proteins have been hampered by the inability t

221 philic system for studying the role of small terminase proteins in viral maturation and presents the

222 Viral pac site-targeting small terminase proteins possess an unusual architecture in wh

223 uires the products of seven viral genes: the terminase proteins pUL15, pUL28, and pUL33; the capsid v

224 activity of the Terminase_1 family of large terminase proteins.

225 We show that IHF and the terminase protomer cooperatively assemble at the cos sit

226 The lambda-terminase protomer is composed of one large catalytic su

227 ion that resembles a "nutcracker" with two L-terminase protomers projecting from the C-termini of an

228 proteolysis of full-length L-terminase (FL-L-terminase) reveals a central seven-stranded beta-sheet c

229 tomers projecting from the C-termini of an S-terminase ring.

230 ows a fold resembling those of bacteriophage terminases, RNase H, integrases, DNA polymerases, and to

231 stranded DNA viruses by a complex of a small terminase (S-terminase) subunit and a large terminase (L

232 This chimeric terminase's ability to package lambdaDNA is reduced appr

233 Terminase's ATPase consists of a classic nucleotide bind

234 ich gpA residues 46 and 84 are important for terminase's high-affinity ATPase activity.

235 ATPase are highly conserved among >200 large terminase sequences analyzed, these may represent common

236 Importantly, the GTA small terminase shares many features with its phage counterpar

237 Sixteen T4-family and numerous phage small terminases show CCMs in the corresponding region of the

238 kaging initiation complex assembled from the terminase small subunit and the packaging region on vira

239 DNA-interacting region on Shigella virus Sf6 terminase small subunit gp1, which occupies extended sur

240 aPI interaction, int (integrase) terS (phage terminase small subunit homologue) and pif (phage interf

241 g phage packaging), interacts with the phage terminase small subunit, forming a heterocomplex.

242 th phage DNA packaging by blocking the phage terminase small subunit.

243 e complexes to identify or design additional terminase-specific compounds.

244 The small terminase subunit (gp16) is inhibitory for packaging lin

245 headful packaging mechanism and encode small terminase subunit (TerS) homologs that recognize the SaP

246 nto the interaction between the larger pUL56 terminase subunit and the smaller pUL89 subunit.

247 Here, we investigated the small terminase subunit from three Podoviridae phages that inf

248 e probed the mechanism of the phage T4 large terminase subunit gp17 by analyzing linear DNAs that are

249 The small terminase subunit has three domains, an N-terminal DNA-b

250 o study the effect of mutations in the large terminase subunit in bacteriophage lambda on packaging m

251 In bacteriophage lambda the catalytic terminase subunit is gpA, which is responsible for matur

252 assemble at the cos site and that the small terminase subunit plays the dominant role in complex ass

253 tein pUL25, the portal protein pUL6, and the terminase subunit pUL33.

254 n, we predicted a classical NLS in the third terminase subunit that is partially conserved among herp

255 que long region 15 (UL15; encoding the large terminase subunit), A374V in UL32 (required for DNA clea

256 only by cos phages, in addition to the large terminase subunit, for cos-site cleavage and melting.

257 copies of a large (TerL) and a small (TerS) terminase subunit.

258 y provided by an ATPase present in the large terminase subunit.

259 viruses by a complex of a small terminase (S-terminase) subunit and a large terminase (L-terminase) s

260 -terminase) subunit and a large terminase (L-terminase) subunit, transiently docked at the portal ver

261 ease, both located in the large terminase (L-terminase) subunit.

262 s in elucidating the structure of individual terminase subunits and their domains, little is known ab

263 ell characterized, little is known about the terminase subunits and their molecular mechanism of DNA

264 The five terminase subunits assemble in a toroid that encloses a

265 of a dodecameric portal and small and large terminase subunits assembled at the special head-tail co

266 ed that both location and potency of NLSs in terminase subunits evolved more rapidly than the rest of

267 Terminase subunits have been studied in-depth, especiall

268 We propose that swapping NLSs among terminase subunits is a regulatory mechanism that allows

269 t that both the pU(L)15- and pU(L)28-bearing terminase subunits mediate docking of the terminase with

270 Previous results indicated that the putative terminase subunits of herpes simplex virus 1 (HSV-1) enc

271 Bioinformatic analysis of large terminase subunits shows that the different functional c

272 al gene assignments included small and large terminase subunits, capsid and tail genes, an N6-DNA ade

273 n the specific DNA interactions of the small terminase subunits, which have support helix-turn-recogn

274 ts lacking functional 80alpha or SaPI1 small terminase subunits.

275 r dynamics simulations of several homologous terminases suggest a novel mechanism, supported by exper

276 The large terminase (TerL) protein contains both enzymatic activit

277 nome and the main motor component, the large terminase (TerL).

278 ts of three components: portal, motor (large terminase; TerL) and regulator (small terminase; TerS).

279 The small terminase (TerS) component of this DNA-packaging machine

280 (large terminase; TerL) and regulator (small terminase; TerS).

281 y a virally encoded molecular machine called terminase that consists of two protein components: A DNA

282 ovir is a small-molecule drug targeting HCMV terminase that is currently in phase III clinical trials

283 ith UL15 and UL33 to form a protein complex (terminase) that is presumed to cleave concatemeric DNA i

284 sphohydrolase and endonuclease activities of terminase, the function of gp3 may be to regulate specif

285 etric association with residues 140-162 of S-terminase, the L-terminase binding domain.

286 ding the ATPase subunit of the DNA-packaging terminase, the only protein with previously verified con

287 ture of the portal and its interactions with terminase, the packaging enzyme, are altered, thus signa

288 le involves the recruitment of an ATP-driven terminase to a unique portal vertex to recognize, packag

289 s a virally encoded molecular machine called terminase to package the viral double-stranded DNA (dsDN

290 and UL89 genes, encoding subunits of CMV DNA terminase, were sequenced from plasma collected from sub

291 y the portal protein, or connector, plus the terminase, which are located at an especial prohead vert

292 erful virally encoded molecular motor called terminase, which comprises large (gp2, 499 residues) and

293 ome by means of a protein complex called the terminase, which is comprised of the HSV-1 UL15, UL28, a

294 Bacteriophage lambda-terminase, which serves as a prototypical genome packagi

295 tiated by the small subunit of the packaging terminase, which specifically binds to the packaging sig

296 The small terminase, which stimulates gp17-ATPase, also stimulates

297 into empty viral procapsids by the phage T4 terminase with high efficiency in vitro.

298 ng terminase subunits mediate docking of the terminase with the portal vertex.

299 petent terminase complex and assembly of the terminase with the portal, in which ring-like protein ol

300 lambda-Terminase with the recognition helix of 21 preferentiall