ライフサイエンスコーパス: thermal denaturation

1 o their denaturation midpoint (e.g., T m for thermal denaturation).

2 artially protect the enzyme's structure from thermal denaturation.

3 Br(2) footprinting, circular dichroism, and thermal denaturation.

4 xes in a 1 M NaCl buffer using UV absorbance thermal denaturation.

5 n unusually low change in heat capacity upon thermal denaturation.

6 cal damage that leads to an earlier onset of thermal denaturation.

7 perature bisulfite ('LowMT') and, sometimes, thermal denaturation.

8 ATP increases the activation barrier towards thermal denaturation.

9 re determined by guanidine hydrochloride and thermal denaturation.

10 uclear magnetic resonance (NMR) during their thermal denaturation.

11 nd was found to stabilize the enzyme against thermal denaturation.

12 oteins, from those folding after chemical or thermal denaturation.

13 is sufficient to produce cold, pressure, and thermal denaturation.

14 analyze the effects of salt and pH on their thermal denaturation.

15 stabilizes this heavy chain fragment against thermal denaturation.

16 d R17H show markedly enhanced sensitivity to thermal denaturation.

17 6 degrees C more stable than the parallel to thermal denaturation.

18 higher transition temperature for rhodopsin thermal denaturation.

19 scular blood and perivascular collagen after thermal denaturation.

20 nhibitors confer enhanced protection against thermal denaturation.

21 as been known to protect other proteins from thermal denaturation.

22 lcium-binding repeats to removal of Ca2+ and thermal denaturation.

23 ecific glycosylation sites were sensitive to thermal denaturation.

24 the stability of both serum albumins against thermal denaturation.

25 antitumor activity and is very resistant to thermal denaturation.

26 e ones traditionally explored in chemical or thermal denaturation.

27 ition of a co-solute, recorded via a delayed thermal denaturation.

28 the double-helical structure of DNA against thermal denaturation.

29 ding protects myosin against heat stress and thermal denaturation.

30 gel electrophoresis, circular dichroism, and thermal denaturation.

31 e the original proteins against chemical and thermal denaturation.

32 lding stability in bulk solution, as well as thermal denaturation, aggregation state, and biological

33 Thermal denaturation analyses by CD and differential sca

34 Circular dichroism spectra in far UV and thermal denaturation analyses demonstrate that axial lig

35 terodimer was confirmed in both chemical and thermal denaturation analyses.

36 Thermal denaturation analysis and protease-based peptide

37 uadruplex was studied by circular dichroism, thermal denaturation, analytical ultracentrifugation, an

38 apo-rHb, rHb-R, and rHb-A were subjected to thermal denaturation and 1H/2H exchange.

39 r characterization, using fluorescence-based thermal denaturation and a crystal structure of the isol

40 nt proteins were significantly more prone to thermal denaturation and aggregation.

41 y of the protein variants, as judged by both thermal denaturation and an unseeded in vitro oligomeriz

42 Biochemical properties such as reversible thermal denaturation and analytical gel filtration data

43 Together, the thermal denaturation and CD studies provide evidence tha

44 Thermal denaturation and chemical denaturation were not

45 Thermal denaturation and circular dichroism (CD) spectro

46 heir physical properties were studied via UV thermal denaturation and circular dichroism spectroscopy

47 Thermal denaturation and circular dichroism spectroscopy

48 Thermal denaturation and circular dichroism studies show

49 alyse the sensitivity of these constructs to thermal denaturation and demonstrate for the first time

50 Using circular dichroism (CD), thermal denaturation and dimethyl sulfate (DMS) footprin

51 mase sequences undergo cooperative two-state thermal denaturation and display very large denaturation

52 ivity of each compound were evaluated in DNA thermal denaturation and DNase I footprinting assays, an

53 We tested six candidates by thermal denaturation and found two highly stabilizing mu

54 N370S GCase was more stable to thermal denaturation and had an increased lysosomal half

55 Thermal denaturation and imino proton solvent exchange e

56 The enzymes were characterized for their own thermal denaturation and inactivation, and they exhibite

57 and ispinesib, we report here the results of thermal denaturation and isothermal titration calorimetr

58 Thermal denaturation and NMR show that the RNA-1 TABS 8m

59 onA can overcome the instability issues from thermal denaturation and nonspecific electrostatic bindi

60 rand conformation using UV- and CD-monitored thermal denaturation and on nucleoside conformation usin

61 allel structure by HTS DNA in the DES, after thermal denaturation and quick cooling to room temperatu

62 Thermal denaturation and refolding monitored by CD ellip

63 Thermal denaturation and refolding on cooling appeared t

64 Thermal denaturation and refolding studies indicated tha

65 structure was formed on the DNA template by thermal denaturation and renaturation, and this hairpin

66 gel electrophoresis, circular dichroism, and thermal denaturation and show that their intra- versus i

67 Using two microbial sequence categories, thermal denaturation and target titration analyses demon

68 that are extremely resistant to chemical and thermal denaturation and that resist proteolysis by comm

69 bic cores exhibit increased stability toward thermal denaturation and unfolding by guanidinium chlori

70 rylamide gels, stabilized the domain against thermal denaturation, and induced docking to PC membrane

71 Chemical cross-linking, thermal denaturation, and size fractionation analyses su

72 4DNA has been obtained from CD spectroscopy, thermal denaturation, and UV-vis titration studies.

73 Residues most stable under thermal denaturation are part of a core, which is assume

74 th selenium and showed a distinct pattern of thermal denaturation as compared with GPx-1 encoded by t

75 A miniaturized thermal denaturation assay was used to screen chemical l

76 ry of cyclic polyamides were measured by DNA thermal denaturation assays and compared to the correspo

77 ate that, at pH 4.0, plasmepsin II undergoes thermal denaturation at 63.3 degrees C.

78 resistant to denaturation by low pH, but its thermal denaturation at pH 3.5 revealed a biphasic trans

79 ed to aqueous solution, pushing the solution thermal denaturation beyond the boiling point of water.

80 Results from thermal denaturation, binding energy, and recognition ex

81 Crystal structures, MANT-GTPgammaS binding, thermal denaturation, biochemical assays and sequence ho

82 nt protein is very stable under chemical and thermal denaturation but shows abnormal Ca(2+) buffering

83 cocerebrosidase that stabilize the enzyme to thermal denaturation by up to 20 K.

84 ent is consistent with stabilization against thermal denaturation caused by additional hydrogen bondi

85 ssays, isothermal titration calorimetry, and thermal denaturation CD spectroscopy, we show that both

86 We compare the thermal denaturation characteristics of double-stranded

87 UV thermal denaturation, circular dichroism, and fluorescen

88 namic analyses of gpW reveal probe dependent thermal denaturation, complex coupling between two denat

89 also significantly stabilize rhodopsin under thermal denaturation conditions, even after lipids are r

90 A thermal denaturation curve recorded at 216 nm showed a m

91 the mass spectral data is used to construct thermal denaturation curves for the detected proteins.

92 Data were compared to simulated thermal denaturation curves to determine estimates for b

93 Two had upshifted thermal denaturation curves when a peptide ligand was pr

94 ein-ligand binding interactions by comparing thermal denaturation data obtained in the absence and in

95 The thermal denaturation data point shows the disulfide brid

96 NMR and thermal denaturation data showed that the replacement wa

97 The thermal denaturation data were analyzed according to the

98 Based on the thermal denaturation data, GB1 stability is affected by

99 ngle-molecule force spectroscopy or existing thermal denaturation data.

100 rmediate states detectable from chemical and thermal denaturation differences in the unfolding free e

101 sized and their DNA interaction evaluated by thermal denaturation, DNA footprinting, and in vitro tra

102 CH1-Zn(2+) undergoes noncooperative thermal denaturation, does not have a near-UV elliptical

103 +)-HIF-1 alpha complex undergoes cooperative thermal denaturation, does produce a near-UV signal, and

104 hich were then characterized with respect to thermal denaturation, enzymatic stability, and fluoresce

105 Use of dynamic light scattering and thermal denaturation experiments delineates the compacti

106 Thermal denaturation experiments demonstrate that T. mar

107 Thermal denaturation experiments find that Sp significan

108 ed by means of surface plasmon resonance and thermal denaturation experiments finding that the positi

109 Thermal denaturation experiments indicate high enthalpic

110 corporated in oligonucleotide sequences, and thermal denaturation experiments indicate that it is des

111 DSC and UV-monitored thermal denaturation experiments indicate that the bival

112 Moreover, thermal denaturation experiments indicated that the melt

113 Results of the thermal denaturation experiments monitored by far-ultrav

114 Thermal denaturation experiments of coiled-coil forming

115 Thermal denaturation experiments of oligonucleotide sequ

116 Analyses of solvent and thermal denaturation experiments provide the cardinal th

117 Thermal denaturation experiments revealed that hm(5)rC i

118 Thermal denaturation experiments show broad transitions

119 (2) FID assay and UV thermal denaturation experiments show that 3 has a highe

120 Circular dichroism spectra and thermal denaturation experiments show that the secondary

121 Thermal denaturation experiments showed that the ternary

122 Thermal denaturation experiments showed Y131A and Y131L

123 Results from thermal denaturation experiments using circular dichrois

124 Their DNA affinity was evaluated by thermal denaturation experiments using salmon sperm DNA.

125 Thermal denaturation experiments were consistent with a

126 ONs and Invader probes are characterized by thermal denaturation experiments, analysis of thermodyna

127 f these structures was evaluated by means of thermal denaturation experiments, finding that the natur

128 folding efficiency, we performed kinetic and thermal denaturation experiments, the results of which d

129 impacting dramatically the interpretation of thermal denaturation experiments, these experimental val

130 rcalator displacement), and UV (ultraviolet) thermal denaturation experiments.

131 z8 was demonstrated by trypsin digestion and thermal denaturation experiments.

132 for DNA which was evaluated by means of DNA thermal denaturation experiments.

133 roximately linearly related to those from CD thermal denaturation for a series of four-helix bundle h

134 degrees C, spectral heterogeneity indicates thermal denaturation for the Na(+)-bound form, whereas s

135 e alpha PNAs with ssDNA has been examined by thermal denaturation, gel electrophoresis, and circular

136 turally occurring protein, with midpoints of thermal denaturation greater than 99 degrees C.

137 Thermal denaturation has irreversible destabilizing effe

138 Differential scanning calorimetry and UV thermal denaturation have been used to determine a compl

139 By thermal denaturation, HII proved to be the least stable

140 tabilized the mutants A104T and M26I against thermal denaturation, improved their ability to scavenge

141 Thermal denaturation in 150 mM KCl indicates that the CR

142 d, in part, by ConA's instability due to its thermal denaturation in the physiological environment (3

143 enaturant shows no heat capacity peak during thermal denaturation, indicating that the transition fro

144 uoresces upon binding to molten globules and thermal denaturation intermediates.

145 bunit analysis reveal that the first step of thermal denaturation involves dissociation of subunits I

146 Rhodopsin thermal denaturation is consistent with the two-state ir

147 displayed even by the repeat proteins whose thermal denaturation is highly cooperative, provided tha

148 itation of several individual domains due to thermal denaturation is reduced upon their fusion into m

149 Using reversible, two-state thermal denaturation, it was found that as these enzymes

150 However, close inspection of chemical and thermal denaturation kinetic experiments in fast-folding

151 Thermal denaturation kinetics demonstrated significant i

152 Thermal denaturation measurements and structural compari

153 We have carried out thermal denaturation measurements on the WT and eight mu

154 CD thermal denaturation measurements revealed that OmpC unf

155 The method is based on the commonly used thermal denaturation method in which ligand binding is r

156 The thermal denaturation method provides a means of measurin

157 hermal titration calorimetry and equilibrium thermal denaturation methods and were found to be signif

158 A wide dispersion of thermal denaturation midpoints that was observed for an

159 Helices were characterized by thermal denaturation, mixing data, and circular dichrois

160 e synthesized, purified, and investigated by thermal denaturation monitored by circular dichroism spe

161 Thermal denaturation monitored by far-UV circular dichro

162 Thermal denaturation, monitored by circular dichroism (C

163 substituted pseudoknots, using UV-monitored thermal denaturation, native gel electrophoresis, and ci

164 log interference with chemical footprinting, thermal denaturation, NMR spectroscopy, and biochemical

165 thermodynamic hypothesis and the process of thermal denaturation, normally thought of as a cooperati

166 By using circular dichroism, thermal denaturation, nuclear magnetic resonance spectro

167 Above its melting temperature, complete thermal denaturation occurs in an activated process.

168 he hairpin starting from their ends, whereas thermal denaturation occurs stochastically.

169 udies were extended to model early stages of thermal denaturation of (Pro-Pro-Gly)10.

170 ar dichroism (CD) spectroscopy monitored the thermal denaturation of 36 heterodimers that generate si

171 age has been shown to physically prevent the thermal denaturation of a number of model proteins.

172 form that can be cooled efficiently to avoid thermal denaturation of antigen.

173 The reversible thermal denaturation of apo alpha-lactalbumin (alpha-LA)

174 e effects of moderate electric fields during thermal denaturation of beta-lactoglobulin were examined

175 del to describe the complex mechanism of the thermal denaturation of CcO, the obtained results were u

176 s molecular dynamics (MD) simulations of the thermal denaturation of chymotrypsin inhibitor 2 (CI2) h

177 cluding pectin depolymerization, by reducing thermal denaturation of depolymerizing enzymes during da

178 ns of targeted DNA sequences purely based on thermal denaturation of DNA heteroduplexes without the n

179 Thermal denaturation of dodecyl maltoside solubilized Cc

180 ble unfolded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs wh

181 ng than the pure types, the amplitude of the thermal denaturation of intracellular proteins was pract

182 In the presence of urea, the thermal denaturation of P61A FIS became concentration de

183 ere we show that, in contrast to WT FIS, the thermal denaturation of P61A FIS was incomplete and yiel

184 At temperatures < 60 degrees C, the greater thermal denaturation of proteins in cutaneous trunci tha

185 The thermal denaturation of proteins in skeletal muscle was

186 wnhill folding have typically focused on the thermal denaturation of proteins that fold near the spee

187 The thermal denaturation of PT S1 was inhibited by its inter

188 In vitro thermal denaturation of purified protein indicated that

189 The thermal denaturation of ribonuclease S and its fragment,

190 Thermal denaturation of the designed dimeric OmpF mutant

191 T4 bacteriophage DNA was investigated using thermal denaturation of the DNA titrated with varying co

192 ility of 1 is demonstrated by monitoring the thermal denaturation of the following double and triple

193 tions leading to stronger networks following thermal denaturation of the globular protein.

194 Thermal denaturation of the human telomerase RNA (hTR) D

195 phenotype of a specific mutation arises from thermal denaturation of the mutant enzyme, the possibili

196 Upon thermal denaturation of the peptide, the amide-I band sh

197 Monitoring fluorescence during thermal denaturation of the protein in the presence of t

198 eaction at temperatures high enough to cause thermal denaturation of the protein substrate.

199 in homogeneous PCR screening assays in which thermal denaturation of the resulting probe-amplicon hyb

200 We found, however, that thermal denaturation of unlabelled wild-type BBL was hig

201 Q, and F92N, with increased stability toward thermal denaturation of which the F71N mutant also showe

202 ular dichroism spectroscopy, used to monitor thermal denaturations of a heterodimerizing leucine zipp

203 to individual ribbons is realized either via thermal denaturation or by addition of a DNA separator s

204 of method is then established by analysis of thermal denaturation or melting of the amplicons.

205 The thermal denaturation parameters convey the changes in st

206 vely little is known about the nature of the thermal denaturation pathway for SA.

207 Data from thermal denaturation plots and CD spectra were less conc

208 sulfoxide which, in conjunction with a short thermal denaturation, prevents renaturation of the duple

209 These results enabled us to profile the thermal denaturation process of these structures to eluc

210 lus (FdTt), we investigated its chemical and thermal denaturation processes in solution.

211 The thermal denaturation profile of MB-FB hybridized with it

212 l electrophoresis, DNA sequence analysis and thermal denaturation profile.

213 CD, NMR, and thermal denaturation profiles are consistent with the fa

214 UV thermal denaturation profiles at different pH values sup

215 Thermal denaturation profiles monitored at 450 nm (P-dC

216 Thermal denaturation profiles of several model oligonucl

217 ing half, even though they exhibit identical thermal denaturation profiles.

218 in vitro using fluorescence polarization and thermal denaturation profiling.

219 Thermal denaturation provides additional support that Ly

220 Thermal denaturation resulted in the gradual destruction

221 n at 298 K and extrapolation to 298 K of the thermal denaturation results at high temperature.

222 o-state transition model to the chemical and thermal denaturation results, ranged from 9.4 to 148 kJ

223 Circular dichroism (CD) spectroscopy and RNA thermal denaturation revealed an increased order and sta

224 mall molecule inhibitors to protect GST from thermal denaturation revealed that compounds with differ

225 Thermal denaturation revealed that lipoprotein stability

226 Protein endothermic transitions (thermal denaturation), rheological properties (protein g

227 re as determined by near UV CD spectroscopy, thermal denaturation, sedimentation equilibrium and velo

228 On the basis of thermal denaturation, sensitivity to chemical denaturant

229 inding K(d) = 3.8 +/- 1.0 muM and reversible thermal denaturation showed that 5 mM calcium stabilized

230 ly by dialysis from 8 M urea and progressive thermal denaturation shows the close apposition and stru

231 tate ensemble identified in a previous 498 K thermal denaturation simulation were quenched under the

232 ding in urea was similar to that observed in thermal denaturation simulations above the protein's T(m

233 Thermal denaturation studies and footprinting experiment

234 (3) UV thermal denaturation studies and ITC experiments show th

235 Thermal denaturation studies and spectroscopic studies w

236 According to the thermal denaturation studies by UV spectroscopy, the eff

237 Thermal denaturation studies conducted optically and by

238 of these compounds by circular dichroism and thermal denaturation studies confirmed their binding mod

239 Thermal denaturation studies demonstrate that, under red

240 Furthermore, thermal denaturation studies detected no significant dif

241 The use of thermal denaturation studies in conjunction with UV spec

242 Circular dichroism thermal denaturation studies indicated that P6981 binds

243 Thermal denaturation studies of the SP-containing oligon

244 Thermal denaturation studies of these normal and damaged

245 Thermal denaturation studies on short DNA hairpins showe

246 Thermal denaturation studies reveal that the SR12813 lig

247 Thermal denaturation studies reveal that these hairpins

248 Thermal denaturation studies revealed that the hydrophob

249 Circular dichroism thermal denaturation studies show that the B-SREBP-1.A-S

250 Thermal denaturation studies showed that at pH 5.0, all

251 Thermal denaturation studies showed that the collagen-li

252 Ultraviolet thermal denaturation studies showed that the duplexes de

253 However, thermal denaturation studies suggest that the originally

254 puter hardware have allowed us to extend our thermal denaturation studies to much lower temperatures.

255 ding for these two solutes are different, CD thermal denaturation studies were employed to dissect th

256 In UV thermal denaturation studies with the poly(dA) x [poly(d

257 sulting from fluorescence binding assays and thermal denaturation studies, demonstrating the ability

258 e core; this conclusion is also supported by thermal denaturation studies.

259 ivity of selected compounds was confirmed by thermal denaturation studies.

260 in different duplexes that were analyzed by thermal denaturation studies.

261 However, a thermal denaturation study using CD and differential sca

262 s more resistant to proteolytic cleavage and thermal denaturation, suggesting that the association of

263 The midpoint of thermal denaturation (T(m)) of Fos-p1 (10 microM) is 30

264 M129C did not affect stability (midpoints of thermal denaturation, T(m) = 65-66 degrees C), whereas t

265 ociation constants determined by alternative thermal-denaturation techniques: TdF or TdCD, and also c

266 in the influence of amino acid additives on thermal denaturation temperature (T(d)) and heat-set gel

267 c protein directly contributes to its higher thermal denaturation temperature (Tm).

268 and DNA strand orientation can increase the thermal denaturation temperature of 17 base-pair duplexe

269 NA-binding affinity, as measured by enhanced thermal denaturation temperature of calf thymus DNA ( T

270 processing alone decreased the enthalpy and thermal denaturation temperature of the connective tissu

271 approximately 15 degrees C difference in the thermal denaturation temperatures (T(m)) of rhodopsin an

272 ve clones were obtained that showed midpoint thermal denaturation temperatures 10-16 degrees C higher

273 o- and holo-Lf in aqueous solution displayed thermal denaturation temperatures of 71+/-0.2 and 91+/-0

274 tion characteristics (average differences in thermal denaturation temperatures of matched vs mismatch

275 The reduced thermal denaturation temperatures of the T61A, S93A, and

276 CPI was more susceptible to thermal denaturation than GCPI as determined by turbidit

277 yosin in type II fibres is more sensitive to thermal denaturation than myosin in type I fibres and th

278 (apo) E4 is less susceptible to chemical and thermal denaturation than the apoE3 and apoE2 domains.

279 fibres were more sensitive to oxidation and thermal denaturation than those from beta-red fibres.

280 Here we show by thermal denaturation that complementary GNA and TNA mixe

281 Further, thermal denaturation that revealed a higher stability of

282 By thermal denaturation, the MOSP(N) and MOSP(C)-like domai

283 During thermal denaturation, the mutant proteins exhibited lowe

284 large-scale simulations of protein folding, thermal denaturation, thermodynamic scan, simulated anne

285 orimetry in combination with scattering upon thermal denaturation to study the unfolding of integral

286 bulk experiments (ultraviolet absorption for thermal denaturation) to analyze the differential stabil

287 d domains were higher than those selected by thermal denaturation (under both neutral and acidic cond

288 xperimental techniques (limited proteolysis, thermal denaturation, urea denaturation followed by puls

289 ween folded and unfolded conformations using thermal denaturation, urea titration, and cation-mediate

290 pecific three-stranded structure is based on thermal denaturation UV-vis and fluorescence studies.

291 '-aminouridine monomers and demonstrate, via thermal denaturation, UV-vis absorption and fluorescence

292 cutive modifications, reflected in decreased thermal denaturation values (DeltaTm, ca. 2.5-11.5 degre

293 Thermal denaturation was used to establish molecular bin

294 The various stages of collagen thermal denaturation were investigated in rat-tendon cry

295 tophan fluorescence, circular dichroism, and thermal denaturation whereas the Q67A and S247A mutants

296 so show a diverse behavior when subjected to thermal denaturation, which is exceptional as all lasso

297 Thermal denaturation, which is used to measure the globa

298 assays: specific activity and resistance to thermal denaturation, which showed that structural chang

299 In this paper, the method of thermal denaturation will be described as it has been ap

300 uations of duplex formation using ITC and UV thermal denaturation with RNA duplexes containing intern