ライフサイエンスコーパス: thermostability

1 ved vaccine immunogenicity, dose sparing and thermostability.

2 hout affecting VcDsbA secondary structure or thermostability.

3 especially if the desired scaffold is of low thermostability.

4 e discovery of mutations that enhance enzyme thermostability.

5 mino acids or positions that likely underlie thermostability.

6 rrelated with their ability to increase NBD1 thermostability.

7 er heat treatment to further assess receptor thermostability.

8 of K(m), k(cat), buffer/serum half-life, and thermostability.

9 binding affinity, proteolytic stability, and thermostability.

10 a molecular basis of NTR contribution to its thermostability.

11 es C to 70 degrees C, indicating fairly high thermostability.

12 ing, and within the affibody to increase its thermostability.

13 es, indicating virus-specific differences in thermostability.

14 er-onset mutations which exhibited increased thermostability.

15 hroughout SERT, 10 were found to improve its thermostability.

16 of the presence of CG, EPI and/or AA on PPO thermostability.

17 d catalytic activity, substrate binding, and thermostability.

18 ated dimer interface possibly reflecting its thermostability.

19 glycosylation events determined increases in thermostability.

20 bB possesses a unique fold that imparts high thermostability.

21 were shown to possess significantly enhanced thermostability.

22 ated dimer interface possibly reflecting its thermostability.

23 of 3-7 to assess changes in C(H)2 and C(H)3 thermostability.

24 eals its contribution to enzyme kinetics and thermostability.

25 ey features that appear to contribute to its thermostability.

26 vitro engineering of proteins with improved thermostability.

27 and potential molecular contributors to its thermostability.

28 s in using D-amino acids to increase protein thermostability.

29 roperties and dramatically increased kinetic thermostability.

30 t changes in secondary structure content and thermostability.

31 cium for binding to calreticulin and reduces thermostability.

32 , which is in contrast with the consensus on thermostability.

33 protein's energy landscape that lead to such thermostability.

34 ting in approximately 14 kJ/mol of increased thermostability.

35 eds more CBH II chimeras share this superior thermostability.

36 sequence that adds 8.5 degrees C to chimera thermostability.

37 eases IDE oligomerization, and decreases IDE thermostability.

38 taining satisfactory specific activities and thermostability.

39 y inefficient turnover of 5FC and/or limited thermostability.

40 hown to decrease K(d) surface expression and thermostability.

41 salt and temperature optima for activity and thermostability.

42 that contribute to TFPA's increased kinetic thermostability.

43 (short isoform) variants exhibiting improved thermostability.

44 ctions that contributed enthalpically to its thermostability.

45 ancestral forms that may contribute to their thermostability.

46 e and charged surface independently imparted thermostability.

47 modified in high yield with no reduction in thermostability.

48 can form a B-form structure and exhibit high thermostability.

49 le others such as the K34Q mutant reduce its thermostability.

50 site lid structure, while fully maintaining thermostability.

51 tiary structure leading to a decrease of its thermostability.

52 wed reduced recognition of CD4 and increased thermostability.

53 polyclonal immune sera without altering its thermostability.

54 n a mixture of subpopulations with different thermostabilities.

55 f individual photobodies possessing distinct thermostabilities.

56 ed receptor (StaR), minimally engineered for thermostability, additional single mutations are then ad

57 We also present thermostability analysis of GCase at pH 7.4 and 5.2 usin

58 five homologous RNase H proteins of varying thermostabilities and enzymatic activities from organism

59 on pairing and, in return, leads to enhanced thermostability and a dramatically reduced (up to 10-30

60 We also observed an improvement in thermostability and a reduction in CD4 affinity.

61 The high thermostability and activity of agave Rca mapped to the

62 This increase in thermostability and alkali tolerance translates to a 4,0

63 h) and spiroiminodihydantoin (Sp) reduce the thermostability and alter the folding of telomeric quadr

64 1984Val variant showing a 2-fold increase in thermostability and an approximately 4- to 8-fold increa

65 Our thermostability and crystal structure studies indicate t

66 screened for novel variants that retain high thermostability and display elevated reverse transcripta

67 nisms for loss of function including altered thermostability and disruptions to alpha helices, disulf

68 quantitative prediction of cellulase chimera thermostability and efficient identification of stabiliz

69 maintained by a selective trade-off between thermostability and enzyme activity.

70 ing mutations conferred similar or increased thermostability and had similar quaternary structure as

71 loid strains that are characterized by lower thermostability and higher frangibility in vitro and str

72 analysis of F45betaY showed that it enhanced thermostability and increased affinity by 60-fold.

73 r to other capsid binders, OBR-5-340 induces thermostability and inhibits viral adsorption and uncoat

74 CysK significantly increases CdiA-CT(EC536) thermostability and is required for toxin interaction wi

75 ins retained tetravalent streptavidin's high thermostability and low off-rate.

76 rmed small plaques, had dramatically reduced thermostability and lower infectivity.

77 n vitro, the mutant actins exhibited altered thermostability and nucleotide exchange rates, indicatin

78 ally modified starch showed a lower range of thermostability and recrystallization temperatures.

79 N-linked glycans of the VWF A2 domain affect thermostability and regulate both the exposure of the AD

80 riable and framework regions (FWR) can alter thermostability and structural flexibility, but their im

81 Correspondingly, K(d) thermostability and surface expression were increased by

82 S and IP changes have only subtle impacts on thermostability and the antigenicity of bNAb and other e

83 peptides, whereas the EGK peptide has lower thermostability and the DGK peptide is the least thermos

84 the sequence and structural basis of protein thermostability and the mechanistic principles of protei

85 The thermostability and the overall secondary structure cont

86 nteraction between the intrinsic property of thermostability and the reactivity of buried free-cystei

87 ift unfolding revealed a correlation between thermostability and the severity of hyperglycemia in the

88 ated extant forms, but they also have higher thermostability and therefore may be excellent tools for

89 eta-sandwich fold, endowing p53 mutants with thermostability and transcriptional activity.

90 Here we study thermostability and use molecular dynamics (MD) simulati

91 e carotenoid deinoxanthin and provides both, thermostability and UV radiation resistance.

92 Our thermostability and X-ray crystallography studies, toget

93 Reptin mutants with altered oligomerization, thermostability, and AGR2 binding properties.

94 gher relative activity at acidic pH, greater thermostability, and better storage stability, compared

95 SdAb possess good solubility, thermostability, and can refold after heat and chemical

96 bits decreased ceftazidime hydrolysis, lower thermostability, and decreased protein expression levels

97 In this study, the hygroscopicity, thermostability, and density of alkylaminium sulfates (A

98 es that contribute to extreme halophilicity, thermostability, and high detoxification capacity, sugge

99 The improved antigenicity, thermostability, and immunogenicity of DS-SOSIP.4mut sug

100 n dependence for cell surface expression and thermostability, and its enhanced susceptibility to path

101 cluding well-established chemical synthesis, thermostability, and low production cost.

102 positional bias has resulted in reduced tRNA thermostability, and may have altered aminoacyl-tRNA syn

103 body formats maintain high expression level, thermostability, and protease resistance.

104 ered substrate specificity profile, enhanced thermostability, and remarkable reactivity toward the de

105 ts suggest that NTR plays a role in XynCDBFV thermostability, and the Cys-4/Cys-172 disulfide bond is

106 N protein of NDV is a crucial determinant of thermostability, and the HN gene from a thermostable NDV

107 ownstream of PET, increases protein folding, thermostability, and tolerance to limited proteolysis.

108 there was a correlation between fitness and thermostability, and we observed that the correlation wa

109 While the overall structure and thermostability are not altered, a subtle increase in th

110 mesophile Escherichia coli revealed that the thermostability arises in part from an unusually low cha

111 plexes with RNA showed slightly lower duplex thermostability as compared to that of the more rigid 3'

112 McjD resulted in reduced ATPase activity and thermostability as shown by circular dichroism, both of

113 on of metal ions to reach the same levels of thermostability as the wild-type protein.

114 s) offer the possibility of improved vaccine thermostability as well as the potential to be safer, mo

115 A thermostability assay indicated that purified tAlv-a1-pH

116 mutagenesis coupled to a radioligand-binding thermostability assay that can be applied to receptors,

117 entification of thermostable mutants using a thermostability assay that is based on binding of an (12

118 ed their stability with a fluorescence-based thermostability assay that monitors protein unfolding wi

119 ocols and advice are given on how to develop thermostability assays for MPs and how to combine mutati

120 Here, we use thermostability assays to demonstrate that the carrier i

121 monstrated by reconstitution experiments and thermostability assays, indicating that the lipid has an

122 eptor, a result that differs from a previous thermostability assessment of the analogous CB(1) mutati

123 ), in a Facade detergent enables radioligand thermostability assessments of this receptor with low ba

124 We used X-ray crystallography, thermostability assessments, and an ERAP1-trimming assay

125 favorable kinetic properties, and excellent thermostability at 37 degrees C.

126 yruvate dehydrogenase complex assay; (ii) in thermostability at 37 degrees C; (iii) in the mechanism

127 bilisation process resulted in higher enzyme thermostability at 45 and 50 degrees C.

128 e demonstrates low catalytic efficiency, low thermostability at temperatures > 40 degrees C, and equi

129 y structure, which is required for increased thermostability but which also prevents active modulatio

130 ain cargoes provided similar enhancements to thermostability, but yielded reduced levels of proteolyt

131 of T(m) = 52 degrees C, enhanced the protein thermostability by 36 degrees C (T(m) = 88 degrees C), w

132 1 amino acid substitutions that improved its thermostability by greater than 7 degrees C without a re

133 his site optimizes both antigen affinity and thermostability by modulating the interdomain conformati

134 mic, and thermodynamic explanation for UVF's thermostability by performing 4 mus of all-atom, explici

135 the population during selection for greater thermostability by sequential treatment at progressively

136 st provide the general background of protein thermostability by specifically focusing on the structur

137 in on bPNA+, similar DNA affinity and hybrid thermostability can be obtained with half the molecular

138 Here we show that a chimera's thermostability can be predicted from the additive contr

139 However, thermostability can vary between isolates regardless of

140 he stability of GP at elevated temperatures (thermostability) can provide insights into its capacity

141 e utilized to determine differences in CB(2) thermostability caused by mutations, detergent compositi

142 e enzyme performance based on differences in thermostability, cellulose-binding domain targeting, and

143 These thermostability changes are sufficiently divergent to al

144 Accurately predicting the protein thermostability changes upon single point mutations in s

145 t GCK proteins were analyzed for kinetic and thermostability characteristics and assigned a relative

146 53C-T142C/T46P mutant xylanase had excellent thermostability characteristics and could be a prospecti

147 ly higher pH of HA activation and reduced HA thermostability compared to the corresponding wild-type

148 HA3-SS has increased thermostability compared to wild-type HA, and binding of

149 ermine whether the underlying mechanisms for thermostability correlate with the changes in T(m), we m

150 We found a large loss in thermostability correlating with the loss of the lipid b

151 This method has been trained on thermostability data for 1231 mutants, the largest publi

152 For the chloride salts, protein thermostability decreases as the size of the peptide inc

153 biocatalysts featuring dramatically enhanced thermostability (DeltaTm = +18.0 degrees C and DeltaT50

154 However, AAO presented different thermostability depending on the electric field strength

155 ported destabilizing mutations had decreased thermostability, did not complement the growth of a temp

156 ealed impaired DNA binding affinity, reduced thermostability, diminished exonuclease activity, defect

157 ips among matrix effect, extractability, and thermostability during in vitro immunodetection using tw

158 pical pharmacokinetics in rodents and retain thermostability, enabling efficient knobs-into-holes bis

159 nce properties, including functional status, thermostability, enzyme activity, and ligand binding aff

160 and the application of selection methods to thermostability evolution have enabled the screening of

161 Therefore, improving chABC thermostability facilitates minimally invasive, sustaine

162 signed based on mathematical modeling of the thermostabilities for the first set of chimeras.

163 We also observed an increase in thermostability for a looped promoter structure.

164 We characterized gene expression and protein thermostability for the three Rca isoforms present in wh

165 al hybridization without compromising duplex thermostability has proven challenging.

166 ve state among which two exhibit an apparent thermostability higher than WT and M23 (a receptor varia

167 ion concerning structure, stoichiometry, and thermostability; how pRNA studies have led to the genera

168 sylation at the catalytic domain affects its thermostability; however, glycosylation of M2 had no eff

169 , D371A, was found to confer (i) substantial thermostability, (ii) an infectivity defect that followe

170 to be in its antagonist conformation and its thermostability improved by earlier limited mutagenesis.

171 n structures enabled the analysis of protein thermostability in a network context.

172 MfdN has large effects on MfdC activity and thermostability in cis, these effects are not observed i

173 expressed in P. pastoris presented increased thermostability in comparison with their unglycosylated

174 actions provides a simple means for evolving thermostability in functional RNAs.

175 ing the benefits provided by its exceptional thermostability in improving molecular biology and genom

176 or Lys mutations in PsbO-Asp157 modify PsbO thermostability in solution, which is consistent with th

177 gp13 exhibits enhanced thermostability in the DNA-filled capsid.

178 se QTY-designed receptors exhibit remarkable thermostability in the presence of arginine and retain l

179 cular disulfide bond contributes significant thermostability in vitro.

180 sized with improved antioxidant activity and thermostability, in comparison to the cyanidin-3-glucosi

181 Although duplex thermostability increases with CG content, the enthalpic

182 The extent to which thermostability influences the location of protein fragm

183 PtdIns5P reduced thermostability, interhelical contacts, and conformation

184 These results indicate that thermostability is a strain-specific feature, measurable

185 idues that influence antibody expression and thermostability is often needed to move promising therap

186 owever, the genetic basis underlying the NDV thermostability is poorly understood.

187 ss, providing evidence that metabolic enzyme thermostability is rate-limiting at superoptimal tempera

188 ing temperature (68.2 +/- 0.6 degrees C) and thermostability (loss of activity after 10-min incubatio

189 yB-Cph1, which based on their small size and thermostability may be useful as cell biological reporte

190 ), SPAD chlorophyll content (SPAD), membrane thermostability (MT), rate of senescence (RS), stay gree

191 properties such as enzyme activity, protein thermostability, mutation position or degree of change i

192 model accurately (R(2) = 0.88) describes the thermostabilities of 54 characterized members of a famil

193 In this study, we analyzed the thermostabilities of 7 H2N3 viruses isolated from mallar

194 ere we attempt to understand how the diverse thermostabilities of bacterial ribonuclease H1 (RNH) pro

195 tive PAGE method to assess the formation and thermostabilities of detergent-solubilized fluorescent p

196 Our results show that the thermostabilities of ferritin-MOFs can be tuned through

197 Whereas the thermostabilities of HjCel3A and Pp-HjCel3A are the same

198 ntributions of various loop sequences to the thermostabilities of parallel-stranded G-quadruplexes.

199 NMR structures indicated that the different thermostabilities of the two 1:2:1 parallel c-MYC G-quad

200 ate the ability of our method to improve the thermostability of a well-behaved native protein.

201 The changes in enzyme kinetics and thermostability of AAO in carrot puree could be related

202 Phe-79 or Tyr decreased the thermostability of actin and increased its nucleotide ex

203 not change, which may be related to greater thermostability of actin and myosin in FPH-8 as observed

204 The thermostability of an integral membrane protein (MP) in

205 The aim of this study was to improve the thermostability of Aspergillus sulphureus acidic xylanas

206 stigated the effects of glycosylation on the thermostability of Bacillus subtilis xylanase A (XynA) e

207 wo most successful strategies to improve the thermostability of beta(1)AR-m23 were an engineered salt

208 O-Mannosylation also increases the thermostability of CBM glycoforms up to 16 degrees C, an

209 Here we show that the average thermostability of class I molecules isolated from cells

210 ea buckthorn extract revealed a satisfactory thermostability of compounds at high temperatures.

211 esearch was to study the enzyme kinetics and thermostability of endogenous ascorbic acid oxidase (AAO

212 biophysical analyses of the pH stability and thermostability of Fcepsilon and IgG1-Fc (Fcgamma).

213 is increasing interest in mechanisms for the thermostability of functional RNA molecules.

214 t loss of conformational epitopes to measure thermostability of GP embedded in viral membranes.

215 budding, while neither rescued the decreased thermostability of H348/352A.

216 actions significantly contribute to the high thermostability of HDL in low-salt solutions.

217 Therefore, variation of thermostability of influenza virus isolates begins at th

218 s spectrometry), despite also decreasing the thermostability of Mb by >10 degrees C.

219 In an effort to increase the thermostability of Moloney Murine Leukemia Virus reverse

220 disulfide bond reduction does not affect the thermostability of monomer SOD1 as significantly as the

221 The thermostability of more than 25 phylogenetically dispers

222 l scanning calorimetry demonstrated that the thermostability of N26D/N131D/N139D SOD1 is lower than W

223 of these chimeric NDVs demonstrated that the thermostability of NDV was dependent on the origin of HN

224 The thermostability of photobodies relies on phyB's photosen

225 etween strain replication efficiency and the thermostability of prion protein aggregates.

226 High thermostability of proteins is a prerequisite for their

227 he detected positive correlation between the thermostability of PrP(Sc) aggregates and disease transm

228 e the impact of DNA methylation marks on the thermostability of regulatory regions.

229 These results indicate that the thermostability of the bacterial collagen is dominated b

230 The thermostability of the biosensors after incubation at 60

231 A remarkable convergence between the thermostability of the capsid and those of the other vir

232 -dependent Raman spectra to characterize the thermostability of the Delta-domain secondary structure

233 Each HR mutation enhanced the thermostability of the endogenous 6HB, potentially allow

234 r ceftazidime to purified PBP3 increases the thermostability of the enzyme significantly and is assoc

235 --> Ser mutation is modeled by the relative thermostability of the Gly-X-Y triplet on the carboxy si

236 faces, and likely contributes to the extreme thermostability of the helical capsid.

237 as defined by NMR; (ii) it induces increased thermostability of the isolated N-terminal domain; (iii)

238 the redox-sensitive toggle that adjusts the thermostability of the linker region to the cell redox s

239 We speculate that low thermostability of the MARTX effector domains correlates

240 Here, the effect of DNA and ATP on the thermostability of the Methanothermobacter thermautotrop

241 We demonstrate that thermostability of the model enzymes haloalkane dehaloge

242 tation increases, rather than decreases, the thermostability of the N-terminal domain of MDM2 in the

243 The thermostability of the PCer-rich phase in the presence o

244 SOD1 zinc derivatives also showed increased thermostability of the protein due to zinc binding.

245 ramer were observed to drastically alter the thermostability of the protein.

246 o proteases, possibly explaining the unusual thermostability of the resulting protease-stalk complexe

247 gp41 resistance mutations that improved the thermostability of the six-helix bundle, but they select

248 leading to dimer formation resulted in poor thermostability of the system, design success was facili

249 The thermostability of these membranes is consistent with th

250 g and mutational analysis suggested that the thermostability of these mutational variants arises from

251 In this work, we analyze the thermostability of three prion strains (BSE, RML and 22L

252 cally improve expression yields and in vitro thermostability of two therapeutically relevant antibodi

253 e femtomolar affinity, low off-rate and high thermostability of wild-type streptavidin.

254 nit that retained the affinity, off rate and thermostability of wild-type streptavidin.

255 Trends in the thermostability of yeast ADP/ATP carrier AAC2 and ovine

256 The temperature-dependent stability (thermostability) of the prefusion conformers of class I

257 study of homologous proteins with differing thermostabilities offers an especially useful approach f

258 es (their dipole moment), confers structural thermostability on double-stranded sequences, and decrea

259 a broad activity range (pH 6-9) and moderate thermostability (optimum ca. 70 degrees C).

260 sulfide-crosslinked enzyme displays improved thermostability, particularly when combined with previou

261 The loop structure and its high thermostability preclude pseudoknot formation.

262 all avidins, shwanavidin also displays high thermostability properties.

263 chrome P450 2C9 (CYP2C9), including elevated thermostability relative to that of CYP2C9, as well as a

264 chain nanostructures also display ultra-high thermostability, resist denaturation by chaotropes and o

265 The pH dependence and thermostability reveal that the enzymes are highly adapt

266 gns on proteins with experimentally measured thermostability show it is beneficial to compute both th

267 Global exchange experiments, supported by thermostability studies, demonstrate that fructose 1,6-b

268 ge rates, affinity for metarhodopsin II, and thermostability suggest that the K345L Galphai1 variant

269 acid pH enhanced, rather than decreased, GP thermostability, suggesting it could enhance viral survi

270 The presence of subpopulations with high thermostability suggests that avian viruses can persist

271 er photosynthetic Fds, although it had lower thermostability (T(m) = 28 degrees C) than did many othe

272 mately 90% alpha-helix), exhibits diminished thermostability (T(m)=40 degrees C; DeltaH(m)=27+/-2 kca

273 es suggest that porcine rotavirus has higher thermostability than human rotavirus.

274 tragonal superstructure exhibits much higher thermostability than its close-packed hexagonal counterp

275 articles with AASs show comparable or higher thermostability than that of AS.

276 , Ago loading of siRNAs is less sensitive to thermostability than that of their shRNA equivalents.

277 nstitutively active CB(2) variant has higher thermostability than the WT receptor, a result that diff

278 s information have been shown to have higher thermostability than their biological counterparts.

279 The tested bnAbs show lower thermostability than their unmutated ancestor antibodies

280 e and acid pH have significant effects on GP thermostability that shed light on their respective role

281 nt of the remaining mutations showed reduced thermostability, the exceptions being the two later-onse

282 red electrophoretic mobility, relatively low thermostability, the presence of atypical disulfide bond

283 , increased mechanical strength and improved thermostability; this resilience should enable diverse a

284 ic technology to improve enzyme function and thermostability through sortase A-mediated crosslinking

285 uble protein expression levels (up 60%), and thermostability (Tm, 12 degrees C higher).

286 steines and secondary mutations that enhance thermostability to achieve a substantial gain in functio

287 ket-specific peptides that showed sufficient thermostability to serve as markers for authentication.

288 at uses GP-specific antibodies to measure GP thermostability under a variety of conditions relevant t

289 ary structures, DtxR(E175K) gains additional thermostability upon activation with metal ions, which m

290 ferences, all of these GPs displayed reduced thermostability upon cleavage to GP conformers (GP(CL)).

291 Moreover, thermostability was enhanced by a hyperstable variant of

292 core versus the fully polar surface to UVF's thermostability, we built two hybrid, chimeric proteins

293 the stability, experimental measurements of thermostability were done using differential scanning ca

294 Changes in thermostability were monitored using differential scanni

295 forms of Rubisco activase (Rca) differing in thermostability, which could be exploited to improve the

296 Fatty-acid cargoes significantly enhanced thermostability while inhibiting cleavage by cathepsin S

297 in, and demonstrated significantly increased thermostability while preserving protein activity.

298 the rational design of enzymes with enhanced thermostability while retaining full enzymatic activity.

299 type hAChE and exhibited 20 degrees C higher thermostability with no change in enzymatic properties o

300 eavage-site mutant viruses displayed reduced thermostability, with double-cleavage-site mutants exhib