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1 mostability and the DGK peptide is the least thermostable.
2 capacity to incorporate iron, and were less thermostable.
3 are monomeric, photochromic, and remarkably thermostable.
4 ow-salt buffer at pH 5.7-7.7, HDL are highly thermostable.
5 SsMTAPII is extremely thermoactive and thermostable.
6 alized by atomic force microscopy) which are thermostable.
7 nase-C for fructose but is considerably more thermostable.
8 atalytic domain of TbCet1 is extraordinarily thermostable.
9 gth F508del-CFTR was about 10 degrees C less thermostable.
11 pt, we have created a fusion consisting of a thermostable aldo-keto reductase, two alpha-helical leuc
13 ty predictably into a completely de novo and thermostable alpha-helical barrel, which comprises seven
16 and biophysical characterization of the most thermostable amylosucrase identified so far, the amylosu
17 y, we show CagY(rpt2) to comprise two highly thermostable and acid-stable alpha-helical structural mo
22 ybrid thermophilic/mesophilic enzyme that is thermostable and more active than the wild-type thermoph
24 and fatty-acid glycerol esters are extremely thermostable and retain internal RNA and DNA oligonucleo
25 d a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to th
26 This enzyme was also found to be remarkably thermostable and was resistant to H2O2 at concentrations
27 n headpiece (HP67) harbors a highly helical, thermostable, and autonomously folding subdomain in the
28 rcSso7d-based binders are highly expressed, thermostable, and monomeric, suggesting that the hyperst
30 nstrate that the Pa-UDGa-Pa-PCNA1 complex is thermostable, and two hydrophobic amino acid residues on
31 icles that are monodispersed, biocompatible, thermostable, and water-soluble, allowing diverse membra
33 olfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-p
34 tion of a highly efficient, promiscuous, and thermostable artificial enzyme that catalyzes a diverse
35 Here the authors show a highly efficient and thermostable artificial enzyme that catalyzes a diverse
36 plex II overproduced in Escherichia coli are thermostable as isolated, but moderately sensitive to re
37 des 1 to 754 were dimeric, with a linkage as thermostable as that between dimers of intact genomic RN
39 The 1D and 2D RIDC3 assemblies are highly thermostable as well, remaining intact at up to approxim
42 showed that folding of the smaller and more thermostable Azoarcus ribozyme is well described by two
45 ing, we established in vitro packaging for a thermostable bacteriophage, P23-45 of Thermus thermophil
49 neous catalysis, yielding high-surface area, thermostable biochemical structures readily adaptable to
50 hich retains actin-binding activity, is more thermostable but, interestingly, does not exhibit the pa
56 nucleases and proteases, this reconstituted thermostable cell-free protein synthesis system may enab
58 . fusca is a model organism for the study of thermostable cellulases and is a major degrader of plant
60 nto a set of general rules for the design of thermostable CHA circuits with high signals and low nois
61 ication processes and demonstrating superior thermostable characteristics and immunogenicity, this st
62 Biochemical and X-ray scattering studies of thermostable CheA from Thermotoga maritima determine tha
65 eries, both cell types express predominantly thermostable class I and are critically dependent on TAP
68 ficant thermal resilience assessed as a high thermostable "coked" fraction in thermal carbon analysis
70 ranslated trespin forms detergent-stable and thermostable complexes with plasmin and elastase but not
71 R domain is capable of forming higher-order, thermostable complexes with VgrG, yet these assemblies f
74 ineered a hemoprotein biocatalyst based on a thermostable cytochrome c that directly transforms alken
76 those in natural collagen and are remarkably thermostable, despite the absence of higher-order associ
80 ar beta-hemolysin phenotype, we surmise that thermostable direct hemolysin (TDH) plays a limited role
81 mutations in S that allow the production of thermostable, disulfide-bonded S-protein trimers that ar
82 of the template, oligonucleotides, salt, and thermostable DNA ligase concentrations increased the spe
84 arious length initiating primers, with three thermostable DNA ligases: Thermus thermophilus (Tth), Th
85 hundreds to thousands of nucleotides by the thermostable DNA polymerase from Thermococcus litoralis
86 ion, we attempted to evolve a high-fidelity, thermostable DNA polymerase to use RNA templates efficie
87 s method to construct genes encoding a small thermostable DNA polymerase, a highly repetitive DNA seq
88 s, we previously reported the evolution of a thermostable DNA polymerase, SFM4-3, that more efficient
91 ately, the relatively high fidelities of the thermostable DNA polymerases commonly used for PCR resul
93 II mutants suggest that, in contrast to the thermostable domains, the thermolabile domains are able
94 ification and characterization of five novel thermostable Dpo4-like enzymes from Acidianus infernus,
96 the consequences of a method that utilizes a thermostable duplex-specific nuclease for reducing the h
97 ller version of N35CCG-N13), and the minimal thermostable ectodomain core of gp41 in its six-helix bu
99 f gp41 fused in helical phase to the minimal thermostable ectodomain of gp41, while the latter compri
102 (C)-like domains of TprC and TprI are highly thermostable, endowing the full-length proteins with imp
103 omologue of ht-ADH, ps-A25Y, leads to a more thermostable enzyme and a change in the rate-determining
104 capable of enhancing the folding yield of a thermostable enzyme at elevated temperature (55 degrees
105 rmus aquaticus DNA polymerase I (Taq Pol), a thermostable enzyme commonly used for DNA amplification
106 aracoccus sp. strain DMF is a halophilic and thermostable enzyme comprising a multimeric complex of t
108 s of Dr-OPH and have found it to be a highly thermostable enzyme, remaining active after 3 h of incub
111 f between enzyme stability and activity with thermostable enzymes being less efficient catalysts that
115 g is a promising source of new organisms and thermostable enzymes that may be helpful in environmenta
116 ragment of DNA polymerase I, and we identify thermostable enzymes that process them efficiently at hi
117 ission maximum of 546 nm, providing a set of thermostable enzymes whose bioluminescence maxima were s
118 lic proteins is a valid strategy to engineer thermostable enzymes with significant low-temperature ac
120 e from representing a potential reservoir of thermostable enzymes, thermophilic fungi are amenable to
127 e on the isolation and characterization of a thermostable F420: NADPH oxidoreductase (Tfu-FNO) from T
129 study, we first created a mutant (mtfl) of a thermostable firefly luciferase (tfl) bearing the peroxi
130 ngle soluble fusion proteins consisting of a thermostable firefly luciferase variant that catalyze ye
135 be grouped into either a thermosensitive or thermostable fraction for both egg- and MDCK-grown virus
136 the proteolytic activity, in particular the thermostable fraction, should be considered as a target
137 for the first time, we engineered CHA to be thermostable from 37 to 60 degrees C and in consequence
139 h an increased lifetime in human plasma, the thermostable GeoCas9 provides the foundation for improve
141 ratures was developed by utilizing a "wired" thermostable glucose-6-phosphate dehydrogenase (tG6PDH)
142 ill allow high-throughput screening of other thermostable glucose-producing enzymes, including those
144 regulates transcription of mgfp5-ER encoding thermostable green fluorescent protein (GFP) or with a s
145 dth of cellular ligands for IFIT5 by using a thermostable group II intron reverse transcriptase for R
148 ve base methylations and a highly processive thermostable group II intron reverse transcriptase to ov
149 odes DMS modifications as mismatches using a thermostable group II intron reverse transcriptase.
151 loits a novel template-switching activity of thermostable group II intron reverse transcriptases (TGI
152 tly capture acceptor RNAs or DNAs, we used a thermostable group II intron RT (TGIRT; GsI-IIC RT) that
153 ermined a crystal structure of a full-length thermostable group II intron RT in complex with an RNA t
155 g a premature termination codon in ORF1 or a thermostable hairpin in the inter-ORF spacer reduces ORF
156 functional in isolation, but together form a thermostable heterooligomeric Rca that can use both alph
157 ophilus (BstHPr) was established as the most thermostable homologue and also the homologue with highe
158 ny rationally designed proteins end up being thermostable, how to intentionally design de novo, therm
159 s basis, a H(2)-powered fuel cell with hyper-thermostable hydrogenase (SHI) as the anodic catalyst wa
161 we demonstrate that lipids render ISVPs less thermostable in a virus concentration-dependent manner.
162 esting that the GP64 trimers were relatively thermostable in the absence of the intermolecular disulf
163 ature isothermal amplification method with a thermostable invertase, we can directly transduce Middle
165 in the target gene and were ligated using a thermostable ligase enzyme only when the genomic DNA car
166 er require tedious purification steps or use thermostable ligases that can generate side products dur
167 ilus P1 lipase provides a template for other thermostable lipases, and offers insight into mechanisms
169 Here, we investigate the properties of the thermostable M/R complex from the archaeon Sulfolobus ac
170 and -2, respectively) were induced to form a thermostable (melting temperature, approximately 90 degr
172 (WT) enzyme, several active site mutants, a thermostable mutant (12X-PTDH), and a thermostable mutan
173 ning with a previously reported red-emitting thermostable mutant and using mutagenesis techniques, we
175 nts, a thermostable mutant (12X-PTDH), and a thermostable mutant with dual cofactor specificity (NADP
177 t factor C5a receptor 1 retrieved 36% of the thermostable mutants in the top 50 prioritized mutants c
179 of states profiles are similar overall, both thermostable mutants show increased populations of the v
181 sient transfection and the identification of thermostable mutants using a thermostability assay that
184 Incubation of excess benzyl alcohol with thermostable N-benzyl-N-nitrosoamides at ambient tempera
185 aryotic thermophiles as source for purifying thermostable native macromolecular complexes with an emp
187 t of thermostability, and the HN gene from a thermostable NDV could be engineered into a thermolabile
190 have cloned, expressed, and characterized a thermostable NOS homolog from the thermophilic bacterium
192 spring metagenomic sampling, and additional thermostable orthologs as a source of natural diversity,
193 ) dependent glucose dehydrogenase (GDH) is a thermostable, oxygen insensitive redox enzyme used in bi
194 I), have been analyzed in the reaction of a thermostable P450, CYP119, with meta-chloroperoxybenzoic
195 ictions, we constructed a family of 44 novel thermostable P450s with half-lives of inactivation at 57
196 ility between the A4V mutant protein and its thermostable parent (HSOD-AS), in which free cysteine re
197 activation temperatures higher than the most thermostable parent CBH II, from Humicola insolens, and
200 roscopy to determine the capsid structure of thermostable phage P74-26 to 2.8- angstrom resolution.
204 led triggers in less than 10 min by means of thermostable polymerase and nicking endonuclease activit
207 imal to best support PCR amplification using thermostable polymerases of a six-letter alphabet that i
208 erase-evolution system and its use to evolve thermostable polymerases that efficiently interconvert C
211 as accessible to enzymatic removal using the thermostable protease isolated from Pyrococcus furiosus.
216 ein mass spectrometric methods to explore if thermostable proteins could be used to identify rendered
220 n more thermally altered samples, so-called 'thermostable' proteins such as osteocalcin which has bee
221 s well as four other cocrystal structures of thermostable PTDH and its variants with different ligand
223 promise to be a source of highly functional, thermostable Rca candidates for thermal fortification of
224 an acid metabolism (CAM) plants must possess thermostable Rca to support Calvin-Benson-Bassham cycle
225 es C exposure, the transgenic lines with the thermostable RCA1 variants exhibited higher photosynthet
228 Using structural information as a guide, a thermostable recombinant chaperonin subunit was modified
229 resent studies on the production of a set of thermostable red- and green-emitting luciferase mutants
230 electrocatalytically reoxidized to NAD by a thermostable redox mediator, osmium (1,10-phenanthroline
231 dapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoauto
235 neered a thermostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control t
236 n this paper, we use conserpin, a synthetic, thermostable serpin, as a model protein with which to in
237 The enzyme was found to be comparatively thermostable showing maximum activity at 60 degrees C fo
238 as good solvent and pH stability and is very thermostable, showing no loss of activity after incubati
240 richia coli In conclusion, FKBP domains from thermostable SlyD proteins are highly suitable as a gene
241 The engineered biocatalyst was shown to be a thermostable, solvent tolerant, and effective cofactor-r
244 ructure of a high fidelity polymerase from a thermostable strain of Bacillus stearothermophilus (Baci
245 owed by 22L (5 log10) while BSE was the most thermostable strain with low or null infectivity reducti
246 aphy, we reveal the assembly principles of a thermostable T = 4 shell topology and its catalytic ferr
247 e Engrailed homeodomain (EnHD) and is highly thermostable (T(m) > 99 degrees C vs. 52 degrees C for E
249 ding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position
250 a isoform based on sequence alignment of the thermostable TaRca1-beta from wheat, OsRca-beta from ric
253 o engineer active P450 enzymes that are more thermostable than any previously made by chimeragenesis,
255 Ruminococcus lactaris is significantly more thermostable than CdiA-CT(EC536), and its intrinsic tRNA
257 beta2 CP assembled at 105 degrees C was more thermostable than either the alpha+beta1+beta2 version a
260 a mutant receptor that is 31 degrees C more thermostable than the wild-type receptor in dodecylmalto
262 roteins from thermophiles are generally more thermostable than their mesophilic homologs, but little
263 uses bearing the TS09-C HN protein were more thermostable than those containing LaSota HN protein.
266 47C, which in the absence of calcium is more thermostable than wild type CjXyn10A bound to the divale
269 s conducted at seven conserved motifs of the thermostable Thermotoga maritima endonuclease V to probe
270 on-PCR (qRT-PCR) assay using the recombinant thermostable Thermus thermophilus (rTth) enzyme was deve
271 NA structural motifs for the construction of thermostable three-dimensional nano-architectures that d
273 97M SOSIP.664 trimers as being appropriately thermostable (Tm of 63.4 degrees C and 62.7 degrees C, r
274 se transcriptases are often not sufficiently thermostable to catalyze synthesis at temperatures high
276 models the final fusion state thought to be thermostable trimers with six-helix bundle structure in
277 iruses by exchanging viral genes between the thermostable TS09-C strain and thermolabile LaSota strai
281 rmance of HDA, we have cloned and purified a thermostable UvrD helicase (Tte-UvrD) and the mutL homol
282 he keys to success were the development of a thermostable vaccine and the application of participator
284 ent the cocrystal structure of an engineered thermostable variant of PTDH bound to NAD(+) (1.7 A reso
288 etergent conditions can support selection of thermostable variants of experimentally demanding GPCRs.
290 -nitrobenzyl esterase (484 residues) and two thermostable variants that were generated by laboratory
291 ides and biomolecules in the design of their thermostable variants where the shielding mechanism can
292 escribe the engineering and improvement of a thermostable viral family A polymerase with inherent rev
293 Chimeras bearing the HN protein derived from thermostable virus exhibited a thermostable phenotype, a
294 A cryo-EM structure of the portal protein of thermostable virus P23-45, determined in situ in its pro
296 ernix bacilliform virus 1) is among the most thermostable viruses known; it infects a hyperthermophil
298 Moreover, crosslinked SCRII was much more thermostable with its denaturation temperature (Tm) incr
300 To overcome this problem, we engineered a thermostable xylanase (XynB) with a thermostable self-sp