ライフサイエンスコーパス: thymi

1 ound (RAG-2(-/-)TAP-1(-/-)OT-1, nonselecting thymi).

2 deficient in Ealpha(6) TCR Tg H-2Malpha(-/-) thymi.

3 mic progenitors (ETPs) relative to wild type thymi.

4 idization between selecting and nonselecting thymi.

5 of spleens (peak at day 7) but were rare in thymi.

6 arathymic lymph nodes were excluded from the thymi.

7 atin in short term organ culture of neonatal thymi.

8 cromolecules to access both murine and human thymi.

9 T cells appeared outside the thymi.

10 d decreased ( P < 0.05) in 12-mo recuperated thymi.

11 ant NKT cells were detected in Ly9-deficient thymi.

12 and was decreased ( P < 0.05) in recuperated thymi.

13 tionated cells isolated from pediatric human thymi.

14 etect class II aggregates in Ii chain mutant thymi.

15 d that CD8(+) T cells populated MRV-infected thymi.

16 les than those isolated from lymph nodes and thymi.

17 BB rats may be absent or deficient in BB rat thymi.

18 le-Tg mice: (1) increased apoptosis in their thymi, (2) remarkable reduction in the proportion of the

19 indle to <5% in FTOC established from day 14 thymi; 3) NK1.1+ cells dominate in FTOC supplemented wit

20 was increased compared to non-islet-bearing thymi (93.7+/-48.6 ng/g tissue vs. 0.7+/-0.4 ng/g tissue

21 ILCs generated in thymi acquire unique homing properties that direct their

22 Bulk RNA-seq of human thymi across the transition also revealed that IGF2 driv

23 c Hoxa3 deletions resulted in small, ectopic thymi, although each had a unique phenotype.

24 In all of the thymi analyzed, alphabeta thymocytes have rearrangements

25 nd tissue-restricted antigens in both intact thymi and cultured thymic epithelial cell line.

26 as similar between autologous and allogeneic thymi and occurred between different cell subsets.

27 y self-reactive T cells are present in their thymi and peripheral lymphoid organs.

28 promoted its pro-apoptotic activity in mouse thymi and small intestines, the chromosomal instability

29 killed 18-24 hrs after study entry, and the thymi and spleen were removed for analysis of apoptosis.

30 s of donor (IAb+) cells were detected in the thymi and spleens of FL-BM recipients.

31 However, these mice also exhibit enlarged thymi and spleens.

32 Tissue, including brains, thymi, and adrenal glands was collected on Day 11.

33 st, infused splenic DCs immigrated poorly to thymi, and did not affect graft survival.

34 y barriers show that allogeneic transplanted thymi are not rejected, and allogeneic cells do not indu

35 direct examination of freshly obtained fetal thymi as well as fetal thymi established in organ cultur

36 In newborn thymi, both pLckCre and endogenous Lck proximal promoter

37 entical subtype (CD8-CD4+) were protected in thymi but not in spleens indicates that cell susceptibil

38 ge in H-2(b) mice (RAG-2(-/-)OT-1, selecting thymi), but are not selected on a transporter associated

39 eneration of CCR10(+)NK1.1(+) ILC1s in adult thymi, but development of CCR10(+)NK1.1(+) ILC1s in neon

40 with tTregs being detected only in neonatal thymi by day 3 after birth.

41 rafts under the kidney capsule and in hybrid thymi by incorporating them into swine thymus (SwTHY) gr

42 re examined for increased apoptosis in their thymi by the TUNEL assay, as well as for loss of HEL-spe

43 tion of SLC/ELC- expression alone in Ltbr-/- thymi can be sufficient to impair thymic negative select

44 Moreover, these findings suggest that fetal thymi contain several novel lymphocyte subsets that can

45 Wild-type thymi contain ~200 small medullae that are connected to

46 ilizing FTOC, we found that: 1) day 12 fetal thymi contained a progenitor that can differentiate into

47 In addition, the NHD13 thymi contained fewer thymocytes, with an increased perc

48 from adult diabetes-resistant BB (BBDR) rat thymi cultured for up to 14 days can adoptively transfer

49 Immunohistochemical staining of pediatric thymi demonstrated the presence of CD20+ B cells and CD1

50 NOD.scid recipients of newborn NOD thymi developed diabetes.

51 -specific responses, and WT recipients of KO thymi developed enhanced responses and a highly exacerba

52 ous stages, we show that fetal and postnatal thymi differ in the frequency and localization of IL-7-e

53 reshly obtained fetal thymi as well as fetal thymi established in organ cultures (FTOC).

54 Histology and flow cytometry on spleens and thymi from 3-week-old pups for T- and B-cell subsets and

55 However, recipients of thymi from 7- and 10-d-old NOD donor mice remained diabe

56 Thymi from aged Tbata-deficient mice are larger and cont

57 Inhibition of glucocorticoid production in thymi from alpha/beta-TCR transgenic mice resulted in th

58 Between 6 and 10 weeks of age, thymi from Bax-expressing mice (either p53+/+ or p53-/-)

59 At 8 weeks posttransplantation thymi from both partially and totally pancreatectomized

60 We show that thymi from Carm1(-/-) embryos (E18.5) have a 5-10-fold r

61 We examined thymocyte emigration in thymi from CXCR4-deficient C57BL/6 embryos in a modified

62 Thymi from different aged NOD mice, representing distinc

63 Thymi from mI-kappaBalpha mice contained increased numbe

64 observations of "misplaced" stromal cells in thymi from multiple species, mimetic cells were first mo

65 Thymi from NHD13 mice were smaller and overexpressed Hox

66 ll, tissues of recipient mice implanted with thymi from NOD mice lacking expression of the autoimmune

67 By contrast, thymi from Rag1(-/-) mice contain as much IL-17A as thos

68 Thymi from Vhlh-deficient mice were small due to a sever

69 In other thymi, gammadelta cells showed no obvious beta gene rear

70 Interestingly, in some thymi, gammadelta thymocytes have out-of-frame beta rear

71 We report that cultured DR- and DP-BB rat thymi generate mature CD4 and CD8 single-positive cells

72 Namely, KO recipients of WT thymi generated reduced IRBP-specific responses, and WT

73 The hyperplastic thymi had normal histology revealing a well-differentiat

74 Accessory cervical thymi have also been identified in humans and mice, and

75 Rare PU.1-/- thymi, however, contained small numbers of thymocytes ex

76 titutively tyrosine phosphorylated in murine thymi in a SAP- and Fyn kinase-dependent manner.

77 compartment in wild-type and Aire-deficient thymi in an effort to integrate the proapoptotic activit

78 Thymi in Id3-deficient mice had aberrant development of

79 Here we show that cervical thymi in mice have following two origins: delayed differ

80 In contrast to thymi in mice with the null allele, the Foxn1(Delta/Delt

81 Co-culture of adult BBDR thymi in the presence of BBDR thyrocytes had no effect o

82 ressing these Vbeta families was observed in thymi in which rat class II+ cells were not detectable.

83 ing gene 1 (RAG-1(-/-)) and TCRbetaxdelta-/- thymi in which T-cell development is blocked at the CD4(

84 g with cleft palates and ectopically located thymi, in Wnt1-Cre alpha5/alphav mutants, suggesting tha

85 Soon after transplantation of wild-type thymi into immunodeficient mice lacking functional T cel

86 that thymocyte development in ADA-deficient thymi is arrested at the DN3-to-DN4 stage transition wit

87 opment of CCR10(+)NK1.1(+) ILC1s in neonatal thymi is less dependent on Delta-like 4-derived Notch si

88 , the cellularity of the spleens but not the thymi is significantly increased compared with that of t

89 This expansion, as in TAP-1o/o thymi, is evident in each of the limited T cell receptor

90 In contrast, recipients of 10 d or older thymi lacked diabetogenic T cells but developed severe c

91 Thymi lacking functional Rho isolated from C3 transgenic

92 e thymic cortex was observed in CCX-CKR(-/-) thymi, likely accounting for their defects in thymocyte

93 We show that wild type thymi maintain their function of T lymphocyte production

94 T cell apoptosis was abundant in thymi of ADA(-/-) mice, but no increase in apoptosis was

95 pontaneous apoptosis is also observed in the thymi of Akt1(-/-) mice, and Akt1(-/-) thymocytes are mo

96 deficient (Gb3S(-/-)) mouse and show that in thymi of alphaGalA(-/-)/Gb3S(-/-) double-knockout mice,

97 s, which are differentially expressed in the thymi of C57BL/6 and 129S6 mice that express the lupus-r

98 om donor mice have the capability to home to thymi of fully allogeneic recipients after intravenous i

99 ro as well as from APCs from the spleens and thymi of hen egg-white lysozyme transgenic mice.

100 contrast, K8(+)K5(+) TECs predominate in the thymi of human CD3epsilon transgenic mice in which thymo

101 recipients., Identification of islets within thymi of hyperglycemic IT recipients was problematic as

102 asmic deletion mutants of IL-7R alpha in the thymi of IL-7R alpha(-/-) mice.

103 Surprisingly, however, although the thymi of IL-7Ralpha Tg mice were comparable at birth, th

104 In contrast, thymi of immunodeficient Rag2(-/-) mice exhibit only ~20

105 Furthermore, the thymi of LIGHT transgenic mice show severe atrophy with

106 The failure of Aire induction in the thymi of lymphotoxin-deficient and lymphotoxin-beta rece

107 increased rate of thymocyte apoptosis in the thymi of M-MuLV-inoculated mice.

108 beta sequences from the CNSs, periphery, and thymi of mice at different stages of autoimmune encephal

109 sed these probes to monitor apoptosis in the thymi of mice treated with dexamethasone as well as in t

110 Thymi of primary donor mice showed reduced cellularity,

111 a-specific T cells were centrally deleted in thymi of progeny that inherited the kappaTg.

112 jected IL-7-secreting stromal cells into the thymi of recipient mice.

113 The thymi of Sh2d3c(-/-) mice showed no maturational abnorma

114 Furthermore, when analyzing whole thymi of T reg TCR Tg RAG-deficient mice, we found signi

115 ) skin gammadelta T cell precursors in fetal thymi of the B6 background mice.

116 n addition, the actual deletional process in thymi of the double-Tg mice was visualized in situ by th

117 Moreover, we find that thymi of TL+ mice congenic or transgenic for H-2T18 also

118 By 14 weeks, the thymi of transgenic mice increased in weight up to 40-fo

119 ling demonstrated increased apoptosis in the thymi of v-cyclin-transgenic mice.

120 lls, undetectable by conventional assays, in thymi of WT (but not of KO) mice.

121 creased in the thymocytes from the atrophied thymi of young Atm-/- mice.

122 CD4(+) T cells purified from thymi or lymph nodes of normal mice prevented the occurr

123 sed with age ( P < 0.001) and in recuperated thymi ( P < 0.05).

124 thoracic thymus, parathyroid-origin cervical thymi (pCT) express low levels of the thymic epithelial

125 Furthermore, almost 40% of PU.1-/- thymi placed in fetal thymic organ culture are capable o

126 ion of PDGFRalpha+ mesenchyme from embryonic thymi prior to their transplantation to ectopic sites re

127 mocyte populations in a number of individual thymi provides evidence for a new pathway of lineage com

128 e conclude that cultured DR-BB and DP-BB rat thymi, respectively, recapitulate the normal and abnorma

129 Microscopic analysis of islet-bearing thymi revealed positive staining for islet-specific horm

130 Finally, an analysis of neonatal thymi revealed that the CD44(lo/int) precursors of gamma

131 Analysis of pediatric human thymi reveals that ZAP70 expression remains low during t

132 Furthermore, RasGRP1/3 double-deficient thymi show significant reductions in ETP numbers compare

133 e report that RasGRP1- and RasGRP3-deficient thymi show significantly reduced numbers of early thymic

134 Repopulated thymi showed a clear increase of CD4-/CD8- and CD8+ frac

135 CD6(-/-) thymi showed a reduction in both CD4(+) and CD8(+) singl

136 e expression profiling of Ly9-deficient mice thymi showed a significant upregulation of IL-4 and prom

137 Five of six NHD13 thymi showed an unusual Tcrb gene rearrangement pattern

138 oes not affect virus titers in infected mice thymi, spleens or infected C1 astrocytes.

139 h either RasGRP1 or RasGRP3 single-deficient thymi, suggesting that both RasGRP1 and RasGRP3 regulate

140 The reconstituted thymi support normal T cell development, and, to a lesse

141 ERKO mice have significantly smaller thymi than their wild-type (WT) littermates.

142 T cells produced by complemented thymi thus functioned normally in vitro and in vivo.

143 ested that the failure of cultured DP-BB rat thymi to generate T cells with a mature phenotype is due

144 II+ antigen-presenting cells remain in their thymi, tolerance will persist as a result of deletion of

145 In contrast, here we show that neonatal thymi transplanted into interleukin 7 receptor-deficient

146 We conclude that neonatal pig thymi transplanted to BALB/c nu/nu mice can support the

147 ation of newborn human CD3epsilon transgenic thymi under the kidney capsule of RAG-1(-/-) mice result

148 The presence of HEL mRNA in mouse thymi was determined by RT-PCR.

149 e marrow chimeras indicated that the smaller thymi were due to a lack of ERalpha in radiation-resista

150 , newborn TCR beta-deficient (TCR beta(-/-)) thymi were grafted to IL-7(-/-) mice.

151 However, when IL-7(-/-) thymi were grafted to TCR beta(-/-) mice, no development

152 Their thymi were smaller, contained significantly fewer cells,

153 ing popliteal lymph nodes [LN], spleens, and thymi) were removed 1, 2, 7, and 14 days later and stain

154 cipients was similar to that in normal mouse thymi, whereas CD4 SP thymocytes in grafts of IIKO mice

155 related orphan receptor gamma (ROR gamma)0/0 thymi, which accumulate immature single-positive (ISP) t

156 rable in day 15 to 16 freshly obtained fetal thymi, which was markedly decreased by day 17 of gestati

157 Transgenic mice showed smaller thymi with a dramatic reduction of CD4+CD8+, CD4+ and CD

158 Thymi with diminished function contain fewer stromal cel

159 unction contain fewer stromal cells, whereas thymi with robust function contain proliferating stromal

160 mma in Nes-expressing cells also had smaller thymi, with reductions in double-negative 4 T cell precu

161 ferentiation origin for a subset of cervical thymi, with specific functional consequences for T-cell

162 SP cells were less abundant in CCX-CKR(-/-) thymi, yet expansion of both DP and immature SP cells wa