ライフサイエンスコーパス: thymine glycol

1 N2-etheno-dG, 1,N2-propano-dG, 8-oxo-dG, and thymine glycol.

2 hich is formed by alkaline hydrolysis of the thymine glycol.

3 nduce the oxidation of the thymine in DNA to thymine glycol.

4 y to single-stranded DNA containing a single thymine glycol.

5 and accurate translesion synthesis past a 5S-thymine glycol.

6 ves oxidized pyrimidines from DNA, including thymine glycol.

7 ic and toxic-oxidized DNA lesions, including thymine glycol.

8 y of HsNTH protein on a substrate containing thymine glycol.

9 pproximately 2-fold reduced global repair of thymine glycol.

10 or undetectable activity with 8-oxoguanine, thymine glycol, 2-hydroxyadenine, hypoxanthine, and xant

11 tructure of a duplex DNA containing a single thymine glycol (5,6-dihydroxy-5,6-dihydrothymidine) has

12 xidatively modified pyrimidine bases such as thymine glycol, 5,6-dihydrouracil, and 5-hydroxypyrimidi

13 rimidine-derived lesions, 5-hydroxycytosine, thymine glycol, 5-hydroxy-6-hydrothymine, 5,6-dihydroxyc

14 5-Hydroxycytosine, thymine glycol, 5-hydroxy-6-hydrothymine, 5,6-dihydroxyc

15 iamino-5-formamidopyrimidine, cis- and trans-thymine glycols, 5-hydroxycytosine, cis- and trans-uraci

16 The 5R thymine glycol (5R-Tg) DNA lesion exists as a mixture of

17 and accurate translesion synthesis past a 5S-thymine glycol (5S-Tg).

18 aining 5,6-dihydrouracil, two enantiomers of thymine glycol, 8-oxo-7,8-dihydroguanine, and an abasic

19 Thymine glycol, a common DNA lesion produced by oxidativ

20 POLN can perform translesion synthesis past thymine glycol, a common endogenous and radiation-induce

21 es RECQL1 on telomeric substrates containing thymine glycol, a replicative blocking lesion.

22 s with the thymine portion being modified to thymine glycol and 5-hydroxy-5,6-dihydrothymine were als

23 enzyme also excised thymine-derived lesions thymine glycol and 5-hydroxy-5-methylhydantoin, albeit a

24 cision of its known major substrates such as thymine glycol and 5-hydroxycytosine.

25 the effects of tandem lesions composed of a thymine glycol and a 5'-adjacent 2-deoxyribonolactone (L

26 lymerase capable to bypass DNA lesions, like thymine glycol and abasic sites, by incorporating direct

27 ase activity against pyrimidine hydrates and thymine glycol and AP lyase activity (DNA strand cleavag

28 A DNA repair activity for thymine glycol and other oxidized pyrimidines has been d

29 ygen species, its spontaneous deamination to thymine glycol and the miscoding property of the latter

30 ude apurinic and apyrimidinic a basic sites, thymine glycol and urea.

31 ycosylase/lyase activity on 5-hydroxyuracil, thymine glycol, and gamma-irradiated DNA with multiple l

32 slesion DNA synthesis opposite an AP site or thymine glycol, and it was recently proposed to be invol

33 toin, 5-hydroxyuracil, 5-hydroxycytosine and thymine glycol, and two purine-derived lesions, 2,6-diam

34 The repair of thymine glycol, another major oxidative DNA base lesion

35 Thymine glycols are formed in DNA by exposure to ionizin

36 ajor oxidative DNA lesions, 8-oxoguanine and thymine glycol, are excised from DNA in vitro by the sam

37 lesion when presented with adenine opposite thymine glycol at the primer terminus.

38 tures with that of a duplex DNA containing a thymine glycol at the same position as the abasic site a

39 Thymine glycol blocks DNA synthesis by most DNA polymera

40 lesion was 5,6-dihydrouracil or cis-(5S,6R)-thymine glycol but not for other lesions tested.

41 reversible complex with the cis-diol of the thymine glycol but not with the urea residue which is fo

42 FANCJ was inhibited by a single thymine glycol, but not 8-oxoguanine, in either the tran

43 The presence of thymine glycol can have significant biological consequen

44 The enzyme cleaved thymine glycol-containing form I plasmid DNA and a dihyd

45 cubated endonuclease III with a 32P- labeled thymine glycol-containing oligodeoxynucleotide in the pr

46 CNBH3, became irreversibly cross-linked to a thymine glycol-containing oligodeoxynucleotide, a reacti

47 ant was also devoid of the ability to incise thymine glycol-containing oligonucleotide, suggesting th

48 ase nicking activities were measured on both thymine glycol-containing oligonucleotides and gamma-irr

49 ntly unwound the DNA substrate harboring the thymine glycol damage in the nontranslocating strand; ho

50 rand-specific inhibition of unwinding by the thymine glycol damage, whereas other DNA helicases (DinG

51 nalysis of nucleotides incorporated opposite thymine glycol demonstrates different misincorporation r

52 esions, 8-oxoguanine, 5-hydroxycytosine, and thymine glycol did not inhibit transcription, although p

53 ves radiolysis products of thymine including thymine glycol, dihydrothymine, beta-ureidoisobutyric ac

54 on, thymine glycol, is removed from DNA by a thymine glycol DNA glycosylase with an apurinic/apyrimid

55 viously purified a bovine pyrimidine hydrate-thymine glycol DNA glycosylase/AP lyase.

56 e generation of mouse strains lacking murine thymine glycol-DNA glycosylase (mNTH1) and/or murine 8-o

57 ressed as a GST-fusion protein, demonstrated thymine glycol-DNA glycosylase activity and, after incub

58 ification of a novel rat liver mitochondrial thymine glycol endonuclease (mtTGendo).

59 ibrium between the cis-5R,6S and trans-5R,6R thymine glycol epimers in duplex DNA was affected by the

60 damage, being able to release both urea and thymine glycol from double-stranded polymers.

61 The edited form removes thymine glycol from duplex DNA 30 times more slowly than

62 n the removal of oxidative damage, including thymine glycols, from the transcribed strand of an activ

63 to bypass 5,6-dihydro-5,6-dihydroxythymine (thymine glycol) generated in two different DNA substrate

64 19T18A17T16G15C14G13G12), with Tg indicating thymine glycol, has been used for these studies.

65 capable of quantifying the rate of repair of thymine glycol in a variety of human cells with a high d

66 s, and there are specific repair enzymes for thymine glycol in a wide range of organisms.

67 ed containing overhangs with 8-oxoguanine or thymine glycol in base-pairing positions of 3-base (-ACG

68 Additional factors that recognize and bind thymine glycol in DNA may be required to ensure RNA poly

69 nsistent with the biological consequences of thymine glycol in DNA.

70 and UvrD) are not significantly inhibited by thymine glycol in either strand.

71 pa inserts the correct base adenine opposite thymine glycol in preference to the other three bases.

72 xyguanosine or uracil-containing duplexes or thymine glycol in single-stranded DNA.

73 osylase or glycosylase/AP lyase activity for thymine glycol in the mitochondrial mouse extracts.

74 Thymine glycol in the nontranscribed strand did not affe

75 We found that thymine glycol in the transcribed strand blocked transcr

76 that mNTH1 is responsible for the repair of thymine glycols in mitochondrial DNA, whereas other glyc

77 Here we have used oligonucleotides with thymine glycol incorporated into different sequence cont

78 The structure shows that the thymine glycol induces a significant, localized structur

79 e efficiency of these enzymes for removal of thymine glycol is about half of that for removal of thym

80 Thymine glycol is generated by both thymine and 5mC, alt

81 c enzyme, whereas its efficiency in excising thymine glycol is lower.

82 that arrest of RNA polymerase elongation by thymine glycol is not necessary for transcription-couple

83 An ionizing radiation-induced DNA lesion, thymine glycol, is removed from DNA by a thymine glycol

84 Oxidative damage, including thymine glycols, is shown to be removed by TCR in cells

85 ficant, localized structural change with the thymine glycol largely extrahelical.

86 In a construct where the minor groove of a thymine glycol lesion faced outward from the histone oct

87 NEIL1 remains catalytically active against a thymine glycol lesion in duplex DNA in vitro.

88 yme correctly extends beyond the site of the thymine glycol lesion when presented with adenine opposi

89 d oligonucleotide duplex containing a single thymine glycol lesion, damage-specific incision at the m

90 rmal primer-termini, when bypassing a single thymine glycol lesion, or when extending certain mismatc

91 tion, although pausing was observed with the thymine glycol lesion.

92 le or pairs of tetrahydrofuran moieties (F), thymine glycol lesions (T(g)) or methylphosphotriester a

93 lity of the enzyme to bypass abasic sites or thymine glycol lesions.

94 y to insert and extend past abasic sites and thymine glycol lesions.

95 DNA substrates containing a single thymine glycol located either in the transcribed or nont

96 residues and can also be used to purify cis-thymine glycol nucleosides.

97 Here we report the effect of thymine glycol on transcription elongation by T7 RNA pol

98 The pyrimidine hydrate thymine glycol, one of many oxidative lesions, can block

99 ucleotides containing an abasic (AP) site, a thymine glycol, or a 5-hydroxyuracil residue as substrat

100 he effect of XPG protein observed in vivo on thymine glycol removal by studying the interactions of X

101 ns can be used to purify DNAs containing cis-thymine glycol residues and can also be used to purify c

102 ines when incorporating a base opposite a 5R thymine glycol stereoisomer.

103 s differ only in the relative proportions of thymine glycol stereoisomers, suggesting that polkappa d

104 nal interaction between RPA and FANCJ on the thymine glycol substrates.

105 aster) than the reported standard substrates thymine glycol (Tg) and 5-hydroxycytosine (5-OHC).

106 Thymine glycol (Tg) and 5-hydroxyuracil (5-OHU) are comm

107 e repair pathways involved in the removal of thymine glycol (TG) from DNA by human cell extracts have

108 clonal antibody that specifically recognizes thymine glycol (Tg) in DNA, we measured the kinetics of

109 Thymine glycol (Tg) is a common product of oxidation and

110 Since thymine glycol (Tg) is non-mutagenic but a strong block

111 Thymine glycol (Tg) is the most common DNA lesion of thy

112 Thymine glycol (Tg) is the most common oxidation product

113 We show that TLS through the thymine glycol (TG) lesion, the most common oxidation pr

114 e activity when the DNA substrate contains a thymine glycol (Tg) opposite adenine (Tg:A).

115 roducts of guanine, and the 5R,6S- and 5S,6R-thymine glycol (Tg) stereoisomers, the most prevalent ox

116 ance, endonuclease III (Nth) does not excise thymine glycol (Tg) when it is part of either tandem les

117 Thymine glycol (Tg), 5,6-dihydroxy-5,6-dihydrothymine, i

118 sine in DNA, followed by deamination, yields thymine glycol (Tg), 5,6-dihydroxy-5,6-dihydrothymine, m

119 Here, we show that telomeric DNA containing thymine glycol (Tg), 8-oxo-7,8-dihydroguanine (8-oxoG),

120 or predominantly error-free replication past thymine glycol (Tg), a DNA lesion formed frequently by f

121 polymerase as is its structural counterpart, thymine glycol (Tg), and to evaluate its pairing propert

122 dative DNA lesions, 8-oxoguanine (8oxoG) and thymine glycol (Tg), regulate the structural properties

123 includes the prototypic oxidized pyrimidine, thymine glycol (Tg), which exists in oxidatively damaged

124 t effect, detected phosphoglycolate (pg) and thymine glycol (Tg).

125 d DNA at sites of oxidized thymine residues (thymine glycol [Tg]).

126 the removal of oxidized pyrimidines, such as thymine glycol, uracil glycol, 5-hydroxyuracil, and 5-hy

127 Thymine glycol was less well tolerated than 8-oxoguanine

128 In contrast, the incision rate of uracil and thymine glycol was not defective in CS cells.

129 elicase activity by the translocating strand thymine glycol was not relieved.

130 [2H4]Thymine glycol was used as an internal standard.

131 use two common DNA lesions, 8-oxoguanine and thymine glycol, were already known to activate repair in

132 We show that TDG and MBD4 can remove thymine glycol when present opposite guanine but not whe

133 Furthermore, nuclear extracts incised thymine glycol with a much higher efficiency than 8-oxoG