ライフサイエンスコーパス: timer

1 y Transfer (FRET) within the tag (named FLIM-timer).

2 ucing the timing factor in maturation of the timer.

3 within a specific window of the sporulation timer.

4 The trans ring thus serves as a variable timer.

5 represents a unique mechanism for a genetic timer.

6 as an evolutionarily conserved developmental timer.

7 logs, tim-1 and kin-20, in the developmental timer.

8 ts, and thus involves a neuroblast-intrinsic timer.

9 sRed, resulted in a mutant named Fluorescent Timer.

10 e of circadian clock genes in the circannual timer.

11 a time course expected if Id4 is part of the timer.

12 rse expected if the proteins are part of the timer.

13 t evidence that p27 is part of the intrinsic timer.

14 r to be controlled by an autonomous maternal timer.

15 eased the fluorescence lifetime of Nrf2-FLIM-timer.

16 may regulate the segmentation clock via the timer.

17 ation of an endogenously programmed interval timer.

18 he use of FLIM-FRET as a readout of the FLIM-timer.

19 cyclin Cdc13 accumulates in the nucleus as a timer.

20 ificantly improved our previous web platform TIMER.

21 ting the effect of the circadian coincidence timer.

22 th, and suggesting a role as a developmental timer.

23 terminal ATPase acts as an input-independent timer.

24 vated caspase-8 with a K48-ubiquitin shutoff timer.

25 d by Hox proteins and an internal neuroblast timer.

26 ing screen identified miR-124 as a candidate timer.

27 rolled by the quantity of wax present in the timer.

28 dely conserved molecular gears of these 24-h timers.

29 echanisms that could potentially function as timers.

30 ed paper and a sheath polymer tape to create timers.

31 mental steps, including molecular clocks and timers.

32 work for the study of emergent developmental timers.

33 Genome Biology, Li and colleagues introduced TIMER, a gene expression deconvolution approach for stud

34 ns of determining the size control strategy (timer, adder or sizer) than the standard method based on

35 Circadian clocks are endogenous timers adjusting behaviour and physiology with the solar

36 This alternating bright/weak banding of Rho-Timer along the length of the ROS relates to inhomogenei

37 This timer also controls daily rhythms in gene expression and

38 scriptional dynamics through flow cytometric Timer analysis.

39 Although mixing a timer and an adder can sometimes attenuate size variatio

40 earable band with an integrated colorimetric timer and biochemical assays that temporally captures sw

41 We propose that PHYC acts as a molecular timer and communicates information on night-length to th

42 Using a fluorescent timer and flow cytometry-assisted organelle sorting, Yau

43 mory of antiDNMT1 to build a signal duration timer and recorder.

44 at the overexpression of p27 accelerates the timer and that the increases in both p27 and p18 that oc

45 e that this feedback loop unites the molting timer and the heterochronic gene regulatory network, pos

46 ights into the ubiquitous attosecond optical timer and the underlying attosecond photoionization dyna

47 ectricity to power small electronic devices (timers and calculators) for several tens of hours.

48 te more complex fluidic controllers, such as timers and clocks.

49 rolling liquid, including autonomous fluidic timers and fluidic logic.

50 Because both the fluidic timers and paper-based assays depend on the wicking rate

51 ic far-red fluorescent proteins, fluorescent timers and photoconvertible fluorescent labels.

52 cently labeled rhodopsin fusion protein (Rho-Timer) and show that rhodopsin incorporation into nascen

53 cell-size homeostasis models are the sizer, timer, and adder [1].

54 r, procaspase-9 autoprocessing activates the timer, and the rate at which the processed caspase-9 dis

55 This model successfully reproduces sizer, timer, and the restriction point features of the eukaryo

56 hore formation in DsRed, TagRFP, fluorescent timers, and PAmCherry.

57 Some timers appear to operate in a largely cell-autonomous fa

58 trol strategies such as sizer-timer or adder-timer are ideal because they maintain constant mean conc

59 Fluidic timers are 97% accurate (with respect to the time requir

60 Timers are drawn at strategic nodes to hold the capillar

61 ed, but the mechanisms underlying biological timers are still unclear.

62 These "fluidic timers" are composed of paraffin wax and a signaling fea

63 isms in plants appear to use their circadian timers as the ruler by which the day/night length is mea

64 is based on a G1 size control and an S/G2/M timer, as found for budding yeast and some human cells,

65 p1 (p27) is a component of this TH-regulated timer, as it increases as OPCs proliferate and is requir

66 the wicking rate of the sample, the fluidic timers automatically calibrate themselves (relative to t

67 mental approaches, including the Fluorescent Timer-based method Timer-of-cell-kinetics-and-activity (

68 s, and suggests an inextricable link between timer-based models of size control and heavy-tailed cell

69 - counteracts protein dilution to facilitate timer behavior.

70 e universality of this so-called coincidence timer, but we lack understanding of the mechanisms invol

71 and that phosphorylation acts as a pseudopod timer by promoting Scar/WAVE turnover.

72 This response requires an endogenous timer called the circadian clock to measure the duration

73 Timers can balance proliferation with differentiation to

74 d its overexpression in OPCs accelerates the timer, causing the cells to differentiate prematurely.

75 the interaction between melatonin-regulated timer cells and adjacent prolactin-secreting cells, whic

76 otein dilution poses stringent challenges to timer circuits by continually diluting out timer compone

77 o timer circuits by continually diluting out timer components in proliferating cells (Figure 1A, righ

78 We demonstrate the use of a fluorescent timer construct, syncollin-dsRedE5TIMER, which changes i

79 that bud growth is likely first sizer- then timer-controlled, that the nuclear concentration of Sfp1

80 e first evidence for an intrinsic cell cycle timer controlling duration and patterning activity of a

81 ating that distributed clocks, not a central timer, coordinate sexual differentiation of the C. elega

82 zation of neutrophils, driven by an internal timer, coordinates immune defense and vascular protectio

83 ysone signal can function as a developmental timer coordinating development within the imaginal disc.

84 Our aim is to standardize Timer data analysis to enhance reproducibility and accur

85 levels of MDM2, thereby suppressing mitotic-timer-dependent G1 cell-cycle arrest.

86 given a time limit of 20 s with a countdown timer displayed at the top of their screen.

87 occurs after reaching a size threshold), and timer (division occurs after a fixed time from birth).

88 ests that a noisy cell-autonomous, intrinsic timer drives the slowing and arrest of oscillations unde

89 In the Ngn3-Timer embryos, green-dominant cells could be readily det

90 in network provides an intrinsic destruction timer, enabling long-range coordination of actin network

91 r' mode in exponential phase to mixed 'adder-timers' entering stationary phase, and then a near-perfe

92 ER-associated degradation (ERAD), acts as a timer enzyme, modifying N-linked sugar chains of glycopr

93 od (an overt expression of the photoperiodic timer) evolves independently of the rhythmic response to

94 ements, indicating that a stimulus-dependent timer exploits arousal to time gaze-shifts.

95 ied the normalization of immature and mature Timer fluorescence data as essential for robust analysis

96 age that automates the data preprocessing of Timer fluorescence data from flow cytometry experiments

97 However, the complexity of Timer fluorescence data has limited its broader applicat

98 ata preprocessing and basic visualization of Timer fluorescence data.

99 Despite their potential, the analysis of Timer fluorescence in flow cytometry is frequently compr

100 By acting as a timer for cell cycle exit, p27(Kip1) curtailed the progr

101 unoccupied XDs of the P tetramer, creating a timer for coordinated polymerase advance.

102 oters may provide an intrinsic developmental timer for dendrite/synapse gene expression.

103 nning proposed a circadian-based coincidence timer for photoperiodic synchronization in plants.

104 undance in freshly harvested seeds acts as a timer for seed dormancy release, which functions largely

105 hosphate release, and to provide an internal timer for the age of the filament.

106 osphorylation of the CII domain provides the timer for the hydrolysis in the CI domain.

107 he peptidyl-prolyl isomerase Pin1 provides a timer for the lifetime of conventional PKC isozymes, con

108 uitary gland contains an endogenous internal timer for the short photoperiod-dependent development of

109 e that ribosome collisions serve as a robust timer for translational quality control pathways to reco

110 n to the challenges associated with building timers from biochemical components.

111 Fast-FT is a fluorescent timer (FT) engineered from DsRed-like fluorescent protei

112 lls has been greatly assisted by fluorescent timers (FT).

113 This timer function arises due to the attenuation of protein

114 spase-9 processing, as well as the molecular timer function of the apoptosome.

115 istinguishing their individual roles in this timer function remains challenging.

116 atile or oscillatory dynamics can facilitate timer functions [3,4].

117 adient modulates the intrinsic dynamics of a timer gene cross-regulatory module, delineating the tail

118 The timer gene provides a simple mechanism to coordinate pat

119 The networks operate through a timer gene, the level of which measures developmental pr

120 netrant bypass of mitosis and of the mitotic timer, generating tetraploid cells arrested in G1.

121 In Drosophila, the timer genes are expressed broadly across much of the bla

122 The neuroblast timer genes hunchback, Kruppel, nubbin and castor are ex

123 lless, but beyond this the regulation of the timer genes is poorly understood.

124 k modelling to resolve the regulation of the timer genes, identifying 11 new regulatory interactions

125 dinated by the sequential expression of the 'timer' genes caudal, Dichaete, and odd-paired, whose exp

126 Timers help cells defer their responses to stimuli, ofte

127 A cell-intrinsic timer helps control when rodent oligodendrocyte precurso

128 An intracellular timer in oligodendrocyte precursor cells is thought to h

129 and Mash1 may be part of the cell-intrinsic timer in the precursor cells.

130 rt of the mechanism that sets the neurogenic timer in these cells.

131 s is similar to sleep regulated by circadian timers in insects and mammals, and sleep in response to

132 ure time and are likely to extend to similar timers in many other precursor cell types.

133 '-endo nucleotides may function as molecular timers in many RNA folding and ligand recognition reacti

134 uggest that miRNAs may act broadly as linear timers in vertebrate neuronal development.

135 rine cells, we developed a mouse model (Ngn3-Timer) in which DsRed-E5, a fluorescent protein that shi

136 cycle once it reaches a critical size, and "timer," in which the cell attempts to grow for a specifi

137 Depending on the order of FPs in the timer, incomplete proteasomal degradation either shifts

138 lengthens the duration of the cell-intrinsic timer, indicating extrinsic factors in the embryo may re

139 ults reveal how an autoregulatory cell cycle timer integrates growth and specification and are widely

140 RFPs with a large Stokes shift, fluorescent timers, irreversibly photoactivatable and reversibly pho

141 We propose that this timer is an innovation of chordates that is also used by

142 The timer is regulated via a special repressilator-like inhi

143 A highlight of this timer is the sharp transition in FGF responsiveness betw

144 An intracellular timer is thought to help control the timing of oligodend

145 that although the striatum serves as a 'core timer', it is part of a distributed timing system involv

146 18/INK (p18), may also be a component of the timer: it increases as OPCs proliferate, and its overexp

147 ed plasma with parallel operations including timers, iterative cycles of synchronous flow and stop-fl

148 FLIM-timer labelled cyclin B was also successfully used to tr

149 Consequently, this results in a timer-like mechanism that ensures the transient nature o

150 hore retention, suggesting an autoregulated, timer-like mechanism.

151 transcription factors and the developmental timer lin-14 cause not only a loss of zig gene expressio

152 models: dimer <--> tetramer <--> hexamer and timer <--> hexamer, equally consistent with the data.

153 w this normalization affects the analysis of Timer maturation.

154 tent with the hypothesis that the circannual timer may reside within the PT thyrotroph and is encoded

155 immunosurveillance to establish a biological timer mechanism that controls cell fate.

156 ng pocket, with such cleavage bursts using a timer mechanism to regulate the RNase activity of the Cs

157 ubiquitin ligase, is a key component of the timer mechanism triggering G1 arrest in response to prol

158 form the basis of the mammalian coincidence timer mechanism.

159 growth and division through sizer, adder, or timer mechanisms or through some combination [1, 2].

160 The cellular timer mechanisms that regulate such changes are, however

161 ence-dependent versus cell-intrinsic genetic timer mechanisms.

162 omes, whereas MAD3 acts as a crucial meiotic timer, mediating a prophase delay in every meiosis.

163 Thus, FLIM-timer methodology increases the FT applicability for vis

164 nderlying the popular "sizer," "adder," and "timer" models of cell homeostasis.

165 nthetic gene network acting as a predictable timer, modifiable by component choice.

166 Removal of the ENSA/Greatwall (EG) timer module eliminates this second threshold, as well a

167 e reduction in fluorescence lifetime of FLIM-timer-Nrf2 confirmed its stabilisation by sulforaphane.

168 oint (sizer) and the size-independent clock (timer) observed in many cell cycle experiments.

169 inesis until exit from mitosis; however, the timer of cytokinesis has not been experimentally defined

170 e cis ring constitutes a second, nonvariable timer of the chaperonin cycle.

171 ues serve, through deamidation, as molecular timers of biological events.

172 tors form dimers, which assemble into stable timers of dimers.

173 lication checkpoint activities are important timers of the undisturbed cell cycle before, but not aft

174 clude that specific assemblies, particularly timers, of naturally secreted Abeta oligomers are potent

175 We previously developed the Timer-of-cell-kinetics-and-activity (Tocky) tools, utili

176 including the Fluorescent Timer-based method Timer-of-cell-kinetics-and-activity (Tocky), analysing t

177 Using the Fluorescent Timer protein, the Timer-of-cell-kinetics-and-activity system enables analy

178 -layer size control strategies such as sizer-timer or adder-timer are ideal because they maintain con

179 ythmicity driven by an endogenous clock-like timer or by other internal or external processes?

180 he circadian clock, our intrinsic biological timer, orchestrates various cellular and physiological p

181 papers, nutrients on food can, and microwave timer (P < 0.05).

182 eveals a biphasic mode of growth: a relative timer phase before constriction where cell growth is cor

183 xplore mixer models of size control, where a timer phase precedes/follows an adder, as has been propo

184 t that depends inversely on the noise in the timer phase.

185 gue that infants and children are well-tuned timers prior to age 5.

186 rocaspase-9 sets the overall duration of the timer, procaspase-9 autoprocessing activates the timer,

187 y regulates the neuroblast temporal identity timer: prolonged Hunchback expression keeps the neurobla

188 activity (Tocky) tools, utilizing a specific Timer protein, Fast-FT, to monitor temporal changes in c

189 Using the Fluorescent Timer protein, the Timer-of-cell-kinetics-and-activity s

190 Here, using a novel fluorescent timer protein, we aimed to investigate the preferential

191 egies based on radiolabeling or fluorescence timer proteins, allowed us to formally demonstrate the p

192 f experimental systems utilizing Fluorescent Timer proteins, including the Tocky tools.

193 Fluorescent Timer proteins, which display time-dependent changes in

194 hod to elucidate the dynamics of Fluorescent Timer proteins.

195 Cellular 'timers' provide an important function in living cells.

196 We conclude that two distinct "timers" regulate neuroblast gene expression: a hunchback

197 Circadian clocks are biochemical timers regulating many physiological and molecular proce

198 owing disassembly to function as a molecular timer, regulating the duration of the telomere open stat

199 rcadian clock and the seasonal photoperiodic timer remains a subject of intense controversy.

200 the possible influences of the moon on ~24-h timers remains scarce.

201 ls expressing the 7.5-kb hPhox2a fluorescent timer reporter differentiated to equal numbers of catech

202 a MitoTimer reporter gene from the existing Timer reporter gene.

203 ore, analysis of wild-type Foxp3 fluorescent Timer reporter mice at different ages uncovered distinct

204 d a novel CRISPR mutant of Foxp3 fluorescent Timer reporter mice lacking the enhancer Conserved Non-c

205 ast gene expression: a hunchback --> Kruppel timer requiring cytokinesis, and a Kruppel --> pdm1 -->

206 striatum effectively paused and rewound the timer, respectively.

207 rlier in the cell cycle affects this mitotic-timer response.

208 I transition functioned as a differentiation timer, restricting the progenitor network to the SHF.

209 le extrinsic factors in the embryo tune this timer's duration and precision.

210 dings support the theory that the neuroblast timer series was co-opted for use in insect segment patt

211 eset buttons, batteries, or maintenance; the timer simply starts once the sample is added to the devi

212 e rather than using an independent 2-hr-long timer started at the beginning of S phase.

213 Characterization of the timer suggests that it might be held inactive in eggs by

214 ction as an intrapituitary "pacemaker-slave" timer system.

215 FLIM-timer-tagged Nrf2 allowed to observe differences in its

216 In addition, a tandem fluorescent timer technology allowed for differential visualization

217 Tandem fluorescent protein timers (tFTs) offer a powerful approach for the assessme

218 Tandem fluorescent protein timers (tFTs) report on protein age through time-depende

219 idence is also emerging for a cell-intrinsic timer that alters the properties of each neuroblast with

220 p27 accumulation is part of a cell-intrinsic timer that arrests the cell cycle and initiates differen

221 regulatory process by acting as a molecular timer that co-ordinates membrane targeting with the synt

222 4C are functionally linked components of the timer that controls abscission in multiple physiological

223 transcription factor is a critical intrinsic timer that controls the onset of SC differentiation by r

224 Previous work identified a developmental timer that controls the stability of cyclin A protein in

225 The circadian clock is the endogenous timer that coordinates physiological processes with dail

226 anism for a genetic circuit to function as a timer that could be used in the engineering of synthetic

227 s demonstrate that p27 is part of the normal timer that determines when oligodendrocyte precursor cel

228 native folding and assembly and setting up a timer that dictates the propensity of misfolded intermed

229 is directly linked to an autonomous maternal timer that drives the early embryonic cell cycles until

230 t Pin1 is a unique, dose-dependent molecular timer that enhances Rta protein function, but inhibits l

231 orphosis suggests that larvae possess a molt timer that establishes a minimal time to metamorphosis.

232 at the Plk1 homolog Cdc5 acts as a molecular timer that facilitates the timely and sequential recruit

233 4 transcription is part of the intracellular timer that helps determine when oligodendrocyte precurso

234 Thus IRE1 activity is governed by a timer that may be important in switching the UPR from th

235 ccessibility is controlled by the biological timer that measures the nucleo-cytoplasmic (N:C) ratio,

236 Here we describe a cell cycle timer that operates in Sonic hedgehog (Shh)-expressing p

237 isomerization acts as an effective molecular timer that plays significant roles in biological and pat

238 early embryo to function as a developmental timer that preserves naivete and prevents premature dele

239 terochronic genes constitute a developmental timer that specifies temporal cell fate selection.

240 The timer that triggers this cell death is yet to be identif

241 Thus, MYC constitutes the GC B cell division timer that when deregulated leads to emergence of B cell

242 stablishment is controlled by the biological timers that control the onset of the MBT.

243 enables BY-kinases to function as molecular timers that coordinate the diverse processes involved in

244 Circadian clocks are endogenous timers that enable plants to synchronize biological proc

245 enabling phytochromes to function as thermal timers that integrate temperature information over the c

246 conclusion that Aux/IAAs are auxin-initiated timers that synchronize developmental transitions.

247 Thus, E5 is a "fluorescent timer" that can be used to monitor both activation and d

248 ulsed positive feedback loop to implement a "timer" that operates over timescales much longer than a

249 This pathway serves as a molecular 'timer' that sets the lifespan of DCs and regulates the m

250 te that cells divide at some threshold size; timers, that cells grow for a set time; and adders, that

251 n, the Galpha subunit's intrinsic activation timer (the rate of GTP hydrolysis) is regulated spatiall

252 een suggested that the cell cycle could be a timer, the underlying mechanisms remain elusive.

253 activate procaspase-3) dictates how fast the timer 'ticks' over.

254 leotide bound to actin filaments serves as a timer to control actin filament turnover during cell mot

255 Thus MAM may function as a timer to couple transcription activation with disassembl

256 This autocrine mechanism could serve as a timer to determine the frequency of pulsatile GnRH relea

257 a library of different chemical tests and a timer to indicate when the tests are completed.

258 nter, but also exploit an intrinsic interval timer to initiate physiological recrudescence following

259 hanism that acts as a cell cycle-independent timer to limit the response to mitogenic signaling and a

260 The trans-proline sets a molecular timer to maintain the binding-active state long enough f

261 the mean and the uncertainty of an internal timer to make near-optimal, time-dependent decisions.

262 as OPCs proliferate and is required for the timer to operate accurately.

263 t Claspin may also respond to an independent timer to trigger the MBT and activation of cell cycle ch

264 r Ypt1p-mediated vesicular transport or as a timer to turn off Ypt1p-mediated membrane fusion but onl

265 roduces the concept of harnessing biological timers to control synthetic materials; and, more general

266 receptor signaling nodes serve as molecular timers to facilitate such discrimination.

267 activities concomitantly with a proteolytic 'timer' to selectively regulate S100A9.

268 ction with a Cdh2 tandem fluorescent protein timer transgenic line, we observed that Cdh2-EGFP molecu

269 ganglia loop may function as an adjustable 'timer,' triggering actions at the desired timing.

270 ngham CANcer data analysis), cBioPortal, and TIMER (Tumor IMmune Estimation Resource).

271 Circadian rhythms and developmental timers utilize distinct timekeeping mechanisms.

272 3) A decision gate that is coupled to the timer via a special repressilator-like loop.

273 zebrafish line with cdh2 tandem fluorescent timer was used to study adherens junctions.

274 ersed in the bath throughout scanning, and a timer was used to synchronize lip movements with the 4D

275 Using chimeric fluorescent timers, we show that synaptic vesicle-associated protein

276 nslation could appear to be a core circadian timer when the true pacemaker is an embedded biochemical

277 de slightly better accuracy than an external timer when used to track an assay that measured the leve

278 The timer, when expressed as a static spatial gradient, func

279 KLHDC2 self-assembly acting like a molecular timer, where only bona fide substrates may bind before E

280 functions as a proteolytic-based 'molecular timer', wherein the intracellular concentration of proca

281 tokinesis, and a Kruppel --> pdm1 --> castor timer which is cell cycle independent.

282 cycle structure is inverted so that G1 is a timer, while S/G2/M performs size control, as is the cas

283 tion module (Spo0A dynamics) is modeled as a timer whose clock rate is adjusted by a stress-sensing u

284 2) A sporulation timer whose clock rate is regulated by cell stress and p

285 havioral phenotypes: Animals that are better timers will also appear less impulsive.

286 differential CDC20 isoform turnover create a timer, with mitotic exit occurring once the truncated Me

287 that the levels of p27 matter in the way the timer works.