ライフサイエンスコーパス: tombusvirus

1 nested genes in plant viruses from the genus Tombusvirus.

2 rified RdRp preparation of Cucumber necrosis tombusvirus.

3 IRV, but not against the related peroxisomal tombusviruses.

4 cation enhancer on the minus-stranded RNA of tombusviruses.

5 s mutant also exhibits strong suppression of tombusvirus accumulation in yeast and in virus-infected

6 Tombusviruses also co-opt pro-viral cytosolic proteins t

7 between fish nodaviruses and members of the tombusvirus and calicivirus groups provide significant n

8 ecrosis virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA gen

9 ecrosis Virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA gen

10 Tombusviruses and a yeast model host are used to identif

11 tion occurs frequently during replication of tombusviruses and carmoviruses, which are related small

12 In this review, we discuss that tombusviruses and nodaviruses are capable of exploiting

13 cuses on our current knowledge obtained with tombusviruses and other plant viruses.

14 This motif is highly conserved among tombusviruses and related carmoviruses, and it is simila

15 Using tombusviruses and the model host yeast, we discovered th

16 Altogether, Rsp5 is a CIRF against a tombusvirus, and it possibly has a regulatory function d

17 ls, similar in ultrastructural appearance to tombusvirus- and togavirus-induced membrane structures.

18 imilar to other positive-strand RNA viruses, tombusviruses are replicated by the membrane-bound viral

19 s in the family should be sought even though tombusviruses are reputed to be soil transmitted without

20 ed broad-spectrum protection against related tombusviruses but remained susceptible to a distantly re

21 cellular Rpn11 is a critical host factor for tombusviruses by regulating viral replication and geneti

22 In summary, evidence is presented that a tombusvirus can use the recombination signal of BMV.

23 ed replication of Carnation Italian ringspot tombusvirus (CIRV) in yeast.

24 In case of Carnation Italian ringspot tombusvirus (CIRV), the mitochondrial membranes supporte

25 ed replication of Carnation Italian ringspot tombusvirus (CIRV).

26 ly, this work has revealed new functions for tombusvirus cis-acting RNA elements and provided insight

27 st proteins present in the cucumber necrosis tombusvirus (CNV) replicase, we affinity purified functi

28 dent replicase complex for Cucumber necrosis tombusvirus (CNV), which is a plus-stranded RNA virus, f

29 monstrate that the minus-strand templates of tombusviruses contain a replication enhancer, which can

30 f cell-intrinsic restriction factors against tombusviruses contains tetratricopeptide repeat (TPR) do

31 eplicase of Cucumber necrosis virus (CNV), a tombusvirus, contains the viral p33 and p92 replication

32 This regulatory mechanism might explain how tombusviruses could adjust the efficiency of RNA replica

33 hese regulatory mechanisms might explain how tombusviruses could adjust the efficiency of RNA replica

34 Surprisingly, tombusviruses could also exploit expanded ER membranes,

35 nthesis and lipid modifications suggest that tombusviruses could create an optimized lipid/membrane m

36 We suggest that tombusviruses could sense the status of the infected cel

37 pose that, in the highly susceptible plants, tombusviruses efficiently subvert the actin network for

38 Tomato bushy stunt virus (TBSV) and other tombusviruses encode a p19 protein (P19), which is a sup

39 We find that in susceptible plants, tombusviruses exploit the actin network for rapid delive

40 Tombusviruses express a 19 kDa protein (p19), which has

41 ements, RII(+)-SL (located internally in the tombusvirus genome) and RIV (located at the 3'-terminus)

42 dium bornovanus As with other members of the Tombusvirus genus, the CNV capsid swells when exposed to

43 As expected, being a member of the Tombusvirus genus, the core structure of CNV is highly s

44 Tombusviruses have been shown to rewire cellular traffic

45 yeast as a model host with bromoviruses and tombusviruses have facilitated the identification of rep

46 lated subcellular membranes, suggesting that tombusviruses have the ability to utilize alternative or

47 ribes the viral replication process based on tombusviruses, highlighting common strategies with other

48 ther, these advances in our understanding of tombusvirus-host interactions are broadly applicable to

49 ndrial CIRV, but not against the peroxisomal tombusviruses in yeast and plants.

50 ron microscopic imaging revealed the lack of tombusvirus-induced spherule-like structures in ESCRT-I

51 ing of vps23Delta yeast revealed the lack of tombusvirus-induced spherule-like structures, while cres

52 NA-dependent RNA polymerase preparation from tombusvirus-infected plants, revealed that this interact

53 ere miR168 is up-regulated, such as during a tombusvirus infection.

54 transgenic Nicotiana benthamiana plants from tombusvirus infections.

55 The p19 protein from the tombusvirus is such a viral suppressor of RNA silencing

56 necrosis virus (CNV), a member of the genus Tombusvirus, is transmitted in nature via zoospores of t

57 of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we have developed a cell-free sys

58 of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we used N-terminal truncated reco

59 ecrosis virus (CNV) is a member of the genus Tombusvirus, of which tomato bushy stunt virus (TBSV) is

60 mensional structure of an siRNA bound to the tombusvirus p19 protein--a suppressor of gene silencing-

61 Tombusvirus p22 emerged through radiation of the widespr

62 Expression of the tombusvirus p33 replication protein in the absence of ot

63 The multifunctional tombusvirus p33 replication protein not only interacts w

64 inhibitory roles of various phospholipids in tombusvirus RdRp activation, we propose that the lipid c

65 Hsp70 function inhibits RNA synthesis by the tombusvirus RdRp in vitro.

66 Demonstration of the ability of carmo- and tombusvirus RdRps to switch RNA templates in vitro suppo

67 llular proteasomal protein, called Rpn11, in tombusvirus recombination in a yeast model host, in plan

68 This suggests that Rpn11p can suppress tombusvirus recombination via facilitating the recruitme

69 gether, the role of multiple RNA elements in tombusvirus replicase assembly could be an important fac

70 repRNA accumulation and the activity of the tombusvirus replicase by up to fivefold.

71 The tombusvirus replicase complex contains heat shock protei

72 and ESCRT-III proteins and the viral RNA in tombusvirus replicase complex formation using in vitro,

73 ement for these co-opted cellular factors in tombusvirus replicase formation.

74 w that the in vitro activity of the purified tombusvirus replicase from gef1Delta yeast was low and t

75 Altogether, tombusvirus replicase in the cell-free system showed fea

76 We found that the template activity of the tombusvirus replicase preparation was stimulated in tran

77 paper reports the first purified recombinant tombusvirus replicase showing high activity and template

78 s a decrease in the in vitro activity of the tombusvirus replicase when isolated from APB-treated yea

79 e viral replicase, (iii) the activity of the tombusvirus replicase, and (iv) the ratio of plus- versu

80 ication protein, its copurification with the tombusvirus replicase, and its presence in the virus-ind

81 al RNA could not be used as templates by the tombusvirus replicase.

82 ion of these lipid enzymes within VROs helps tombusviruses replicate in an efficient milieu.

83 and degradation of proteasome substrates, in tombusvirus replication and recombination in Saccharomyc

84 und that the cellular Rpn11 is subverted for tombusvirus replication and Rpn11 has a proteasome-indep

85 eveloped ER and mitochondrion-based in vitro tombusvirus replication assays.

86 teins act as CIRFs and also as regulators of tombusvirus replication by inhibiting the assembly of ne

87 all, the current work provides evidence that tombusvirus replication could occur in vitro in isolated

88 re involved in determining the efficiency of tombusvirus replication in the two subcellular membranes

89 4), Vps20p, and Vps24p ESCRT-III proteins in tombusvirus replication in yeast and in vitro.

90 ligase and its WW domain in plant-infecting tombusvirus replication in yeast cells and in vitro usin

91 yeast cell extract and purified recombinant tombusvirus replication proteins to show that RII(+)-SL,

92 opsis homologs of Vps23p and Bro1p inhibited tombusvirus replication to greater extent than individua

93 n yeast or plant leaves led to inhibition of tombusvirus replication, confirming that CypA is a restr

94 our understanding of the role of sterols in tombusvirus replication, in this work we showed that the

95 nase (GAPDH), two host factors with roles in tombusvirus replication.

96 ew focuses on virus-host interactions during tombusvirus replication.

97 a systematic deletion approach with a model tombusvirus replicon RNA in Saccharomyces cerevisiae, wh

98 d down or a mutated Rpn11 is expressed, then tombusvirus RNA goes through rapid viral recombination a

99 ast genes) that affected the accumulation of tombusvirus RNA.

100 A comparison of different tombusviruses showed, in each case, conservation for pot

101 By using tombusviruses, small model viruses of plants, we dissect

102 An emerging concept based on tombusviruses, small plant viruses, is that viruses migh

103 In summary, tombusviruses target a major crossroad in the secretory

104 Previous studies with tomato bushy stunt tombusvirus (TBSV) in a yeast model host have revealed t

105 Previous work with Tomato bushy stunt tombusvirus (TBSV) in model host yeast has revealed esse

106 Tomato bushy stunt tombusvirus (TBSV) is a model virus that can replicate a

107 ave been found to inhibit Tomato bushy stunt tombusvirus (TBSV) replication in a Saccharomyces cerevi

108 Previous works with Tomato bushy stunt tombusvirus (TBSV) revealed the recruitment of either pe

109 een shown to affect RNA-RNA recombination in tombusviruses; this opens an opportunity to study the ro

110 d subcellular membranes might be utilized by tombusviruses to regulate new VRC assembly during the co

111 ose that subversion of Rab7 into VROs allows tombusviruses to reroute endocytic and recycling traffic

112 In this study, we used a plant RNA virus, tombusvirus, to examine the role of a cellular proteasom

113 Using the prototypical tombusvirus, Tomato bushy stunt virus (TBSV), we show th

114 me AMMECR1, the siRNA silencing repressor of tombusviruses, tRNA Wybutosine biosynthesis enzyme Tyw3p

115 ated with Tomato bushy stunt virus (TBSV), a tombusvirus, undergoes frequent recombination in plants

116 also functions in the proper assembly of the tombusvirus VRCs.

117 Replication of the RNA genomes of tombusviruses, which are small plus-sense RNA viruses of

118 oped an in vitro trans-replication assay for tombusviruses, which are small plus-strand RNA viruses.

119 Tomato bushy stunt virus (TBSV), a tombusvirus with a nonsegmented, plus-stranded RNA genom