ライフサイエンスコーパス: trans-splicing

1 other than chromosomal rearrangement (e.g., trans-splicing).

2 partners of the split intein (called protein trans-splicing).

3 ions in polycistronic RNA precursors through trans splicing.

4 -3 and -4 can lead to a 20-fold decrease in trans splicing.

5 ligo-U 3' extension that is removed prior to trans splicing.

6 SR proteins in splice site communication in trans splicing.

7 ation, excluding the presence of operons and trans-splicing.

8 n, including polycistronic transcription and trans-splicing.

9 mentary binding domain to position mRNAs for trans-splicing.

10 of C. elegans and other species that use SL2 trans-splicing.

11 reversing the roles of snRNP and pre-mRNA in trans-splicing.

12 transcriptome of C. elegans for patterns of trans-splicing.

13 (m(2,2,7)G) cap derived from spliced leader trans-splicing.

14 g elements and could favor cis-splicing over trans-splicing.

15 n the phenomenon of spliceosome-mediated RNA trans-splicing.

16 c screen and shown to be defective for rps12 trans-splicing.

17 st a framework for DNA recombination and RNA trans-splicing.

18 to Igmu/BCL6 CT and were not generated from trans-splicing.

19 rapy could be overcome by pre-mRNA segmental trans-splicing.

20 nown as exon scrambling, exon repetition and trans-splicing.

21 ed an essential function at an early step in trans-splicing.

22 anistic link between cap 4 modifications and trans-splicing.

23 15 ESTs representing five types of putative trans-splicing.

24 in organisms featuring a cap 4 structure and trans-splicing.

25 e natural regulation of CD40L using pre-mRNA trans-splicing.

26 ct migrated to the chloroplast and underwent trans-splicing.

27 in ligated aadA-smGFP due to In-Ic-mediated trans-splicing.

28 phyla involves spliced leader (SL) addition trans-splicing.

29 may involve the production of joint RNAs by trans-splicing.

30 identified that are involved uniquely in SL trans-splicing.

31 ive splicing, and (4) multiple promoters and trans-splicing.

32 t mRNAs in Hydra receive leader sequences by trans-splicing.

33 of a complex containing CstF-64, but not for trans-splicing.

34 quence is added to the 5'end of each mRNA by trans-splicing.

35 plicing reactions is essential for effective trans-splicing.

36 ith SL1 RNA and related SmY RNAs, impairs SL trans-splicing.

37 otein assembly, have an important role in SL trans-splicing.

38 hotosystem I subunit A involves two steps of trans-splicing.

39 stronic transcription and mRNA processing by trans-splicing.

40 transcripts into close proximity to promote trans-splicing.

41 xpands the scope of applications for protein trans-splicing.

42 proteins known to be involved in nematode SL trans-splicing.

43 at the 5' terminus that usually accompanies trans-splicing.

44 he intron underwent a transition from cis to trans splicing 15 times among the sampled angiosperms.

45 In trans-splicing, a common exon, the spliced leader, is ad

46 mall viral packaging capacity of the vector, trans-splicing AAV vectors are able to package twice the

47 Spliced leader (SL) RNA trans splicing adds a trimethylguanosine (TMG) cap and a

48 encing led to a block of spliced leader (SL) trans splicing, an essential step in trypanosome mRNA ma

49 bstitution in the absence of ZnCl(2) blocked trans splicing and decreased C-terminal cleavage kinetic

50 ximately 20-bp element is sufficient for SL2 trans splicing and mRNA accumulation when transplanted t

51 However, recent discoveries of trans-splicing and cis-splicing events between neighbori

52 eins were multimeric, with the efficiency of trans-splicing and extent of multimer expression decreas

53 nding of these factors to pre-mRNA regulates trans-splicing and hence expression of specific genes.

54 nciples and latest work in bifunctional RNA, trans-splicing and modification of U1 and U7 snRNA to ta

55 vidual mRNAs are generated by spliced leader trans-splicing and polyadenylation, processes that are f

56 and long non-coding RNAs (lncRNAs), undergo trans-splicing and polyadenylation.

57 polyadenylate non-coding RNAs, which undergo trans-splicing and polyadenylation.

58 ia genes and detected widespread alternative trans-splicing and polyadenylation.

59 hat are dissected by the concerted action of trans-splicing and polyadenylation.

60 fs in the untranslated regions regulate mRNA trans-splicing and RNA stability, yet where UTRs begin a

61 p codons, as well as high levels of editing, trans-splicing and the addition of oligonucleotide caps

62 ed through the discovery of highly efficient trans-splicing and trans-cleaving inteins.

63 aturation in Trypanosoma brucei depends upon trans splicing, and variations in trans-splicing efficie

64 logy, including polycistronic transcription, trans-splicing, and a glycosome-like organelle.

65 As: lariat introns, Y-structure introns from trans-splicing, and circular exons through exon skipping

66 ed mutants in sna-1 that are defective in SL trans-splicing, and demonstrate that reducing function o

67 in vivo evidence for proteins involved in SL trans-splicing, and indicate that continuous replacement

68 methods-expressed protein ligation, protein trans-splicing, and native chemical ligation-each have s

69 ost protein-coding transcripts is removed by trans-splicing, and noncoding initiation sites have not

70 f gene pieces, joining of precursor RNAs via trans-splicing, and RNA editing by substitution and urid

71 element expansion, horizontal gene transfer, trans-splicing, and simplification of gene structure and

72 ering more efficient vectors for dual-vector trans-splicing approaches.

73 Here, we successfully demonstrate RNA trans-splicing as an auspicious repair option for mutati

74 ein (CL intein) that is capable of efficient trans-splicing at ambient temperatures, without a denatu

75 antibodies, "Bispecific Antibody by Protein Trans-splicing (BAPTS)".

76 Thus, the trans-splicing between CoAA and RBM4 transcripts may rep

77 d that the switched alternative splicing and trans-splicing between CoAA and RBM4 transcripts result

78 er, identify 80 genes that appear to undergo trans-splicing between homologous alleles and can be cla

79 Our results suggest that trans-splicing between homologous alleles occurs more co

80 ears to arise from physiologically regulated trans-splicing between precursor messenger RNAs for JAZF

81 It is exceedingly rare to have trans-splicing between protein-coding exons and has been

82 which exon repetition occurs as a result of trans-splicing between separate pre-mRNA transcripts fro

83 on in the mod(mdg4) gene is a consequence of trans-splicing between two different mutant transcripts.

84 agents severely limits the scope of protein trans-splicing by excluding proteins sensitive to reduci

85 However, efficient trans splicing can be restored by inserting a second AG

86 Trans-splicing/cleavage reactions were initiated by comb

87 The characterization of this psaA trans-splicing complex is also of interest from an evolu

88 d, including inter-chromosome translocation, trans-splicing, complex chromosomal rearrangements, and

89 Spliced leader (SL) RNA trans-splicing contributes the 5' termini to mRNAs in a

90 In conclusion, RNA trans-splicing could provide the basis of therapeutic st

91 We have used an in vitro trans-splicing/cross-linking system in Saccharomyces cer

92 ual-vector sets had a significantly enhanced trans-splicing efficiency (6- to 10-fold, depending on t

93 pends upon trans splicing, and variations in trans-splicing efficiency could be an important step in

94 Y) tract with cytidine resulted in increased trans-splicing efficiency, whereas purines led to a larg

95 ent a plasmid-based system in which upstream trans-splicing efficiently generates mRNAs that encode h

96 olyadenylated downstream mRNA, and that this trans-splicing event negatively impacts the biogenesis o

97 independent ESTs represent the same putative trans-splicing event.

98 formed by either fusion genes (DNA level) or trans-splicing events (RNA level).

99 The trans-splicing events uncovered in Austrobaileyales, eum

100 ghput approaches cannot distinguish cis- and trans-splicing events, the extent to which trans-splicin

101 Its maturation requires two cis- and two trans-splicing events.

102 s splicing demonstrate that PPR4 is an rps12 trans-splicing factor.

103 the feasibility of spliceosome-mediated RNA trans-splicing for imaging gene expression at the level

104 e or decrease in E10 content was achieved by trans-splicing from a target carrying the Delta280K muta

105 Metazoan spliced leader (SL) trans-splicing generates mRNAs with an m(2,2,7)G-cap and

106 es for investigating molecular mechanisms of trans-splicing, generating covalent protein multimers wi

107 As model system, a trans-splicing group I intron ribozyme was evolved in Es

108 SL trans-splicing has been documented in a limited but dive

109 een downstream location in an operon and SL2 trans-splicing has been strong, but anecdotal.

110 Split intein-mediated protein trans-splicing has found extensive applications in chemi

111 Despite this, the precise details of SL trans-splicing have yet to be elucidated.

112 ates that both proteins are essential for SL trans-splicing; however, neither protein is required eit

113 f the Ssp DnaE intein with ZnCl(2) inhibited trans splicing, hydrolysis-mediated N-terminal trans cle

114 analyze sequence requirements for efficient trans splicing in the poly(Y) tract and around the 3'SS,

115 es its ability to screen for rare intragenic trans-splicing in any target gene with a large backgroun

116 Spliced leader (SL) trans-splicing in Caenorhabditis elegans attaches a 22-n

117 report the detection of spliced leader (SL) trans-splicing in calanoid copepods.

118 e yeast cells by using intracellular protein trans-splicing in combination with a highly efficient sp

119 We uncovered the SL trans-splicing in copepod natural populations, and demon

120 ncing of mRNA to identify genes that undergo trans-splicing in Drosophila interspecies hybrids.

121 nents followed by post-translational protein trans-splicing in Escherichia coli generated a fully fun

122 biological significance and evolution of SL trans-splicing in eukaryotes.

123 ubstrate spliced leader RNA transcription or trans-splicing in Leishmania tarentolae.

124 FP-based reporter assay that can monitor SL1 trans-splicing in living C. elegans.

125 y much higher than for examples of bona fide trans-splicing in mammals.

126 vel developmental paradigms for the study of trans-splicing in metazoans.

127 ive effect of a downstream 5' splice site on trans-splicing in nematode extracts containing either in

128 Here, we demonstrate intein trans-splicing in Nicotiana tabacum chloroplasts by usin

129 te that several inteins can catalyze protein trans-splicing in tens of seconds rather than hours, as

130 Trans-splicing in the Drosophila melanogaster modifier o

131 ork for the coupling of 3' end formation and trans-splicing in the processing of polycistronic pre-mR

132 NP onto the branch site as well as efficient trans-splicing in these inactive extracts could be rescu

133 Trans-splicing in trypanosomes and nematodes has been ch

134 g that U1 recruitment is critical to promote trans-splicing in vivo.

135 , mRNAs are processed by spliced leader (SL) trans splicing, in which a capped SL, derived from SL RN

136 hese findings represent a novel mechanism of trans-splicing, in which RNA motifs in the 5' intron are

137 A naturally occurring trans-splicing intein from the dnaE gene of Synechocysti

138 splicing were investigated by employing the trans-splicing intein from the dnaE gene of Synechocysti

139 T7 endonuclease I was produced by means of a trans-splicing intein system.

140 base editors that are then reconstituted by trans-splicing inteins.

141 Trans-splicing introduces a common 5' 22-nucleotide sequ

142 Protein trans-splicing involving naturally or artificially split

143 Thus E10 inclusion can be modulated by trans-splicing irrespective of the strength of the cis-s

144 Spliced leader (SL) trans-splicing is a biological phenomenon, common among

145 Spliced leader (SL) trans-splicing is a critical element of gene expression

146 It has also been suggested that trans-splicing is a mechanism for the generation of chim

147 Spliced leader trans-splicing is an mRNA maturation process used by a s

148 e, a potentially ubiquitous reaction such as trans-splicing is not consistent with a phenomenon that

149 R proteins and suggest that plastid group II trans-splicing is performed by different machineries in

150 another function of flatworm spliced leader trans-splicing is to provide some recipient mRNAs with a

151 The chemical mechanism of protein trans-splicing is well-understood and has been exploited

152 Unlike typical cis-splicing, trans-splicing joins exons from two separate transcripts

153 Dual trans-splicing lacZ vectors were used to functionally ev

154 ctures and encode transcripts that display a trans splicing leader at the 5' end.

155 Thus, HIGM1 can be corrected by CD40L trans-splicing, leading to functional correction of the

156 de the first in vivo demonstration that SMN2 trans-splicing leads to a lessening of the severity of t

157 ito lays in a single strand, suggesting that trans-splicing may have originated in the Drosophila lin

158 The use of protein trans-splicing may help facilitate exciting new avenues

159 Alternative cis- or trans-splicing may overcome the need for genomic structu

160 dings provide new opportunities to study how trans-splicing may regulate the phenotype of a cell.

161 hereby signifying an intein-mediated protein trans-splicing mechanism reconstituted in plant cells.

162 It is capable of catalyzing a protein trans-splicing mechanism to assemble a mature protein fr

163 and wild-type muscles, a comparable level of trans-splicing-mediated beta-galactosidase expression wa

164 AAV concatemers may have contributed to the trans-splicing-mediated transgene expression.

165 A pre-trans-splicing molecule (PTM) corrected endogenous FVIII

166 nactivating (SIN) lentiviral vector a 3' RNA trans-splicing molecule, capable of replacing COL7A1 exo

167 nature of the sequences delivered by the pre-trans-splicing molecule.

168 Pre-trans-splicing molecules (PTMs) were engineered to capit

169 Trans-splicing molecules designed to increase exon 10 in

170 Because of the modular design of pre-trans-splicing molecules, there is great potential to em

171 frameshift was required for splicing or that trans splicing occurred.

172 Trans-splicing occurred maximally at pH 7.0, while a sli

173 Trans-splicing occurred whether both intein fragments we

174 In this system, trans-splicing occurs between one of two downstream spli

175 d trans-splicing events, the extent to which trans-splicing occurs between protein-coding exons in an

176 SL2 trans-splicing occurs in order to separate the products

177 functional mRNAs in a process that requires trans splicing of a capped 39-nucleotide RNA derived fro

178 nating its 5'-terminal region in SL addition trans splicing of nuclear pre-mRNA.

179 In all trypanosomatids, trans splicing of the spliced leader (SL) RNA is a requi

180 onventional cis-splicing or for bimolecular (trans-) splicing of fragmented cis constructs.

181 Through trans-splicing of a 39-nt spliced leader (SL) onto each

182 The cap 4 becomes part of the mRNA through trans-splicing of a 39-nucleotide-long sequence donated

183 pm(6,6)AmpAmpCmpm(3)Ump-SL RNA) conveyed via trans-splicing of a universal spliced leader.

184 omplexes (I and II) that are responsible for trans-splicing of either psaA-i1 or psaA-i2.

185 Trans-splicing of eri-6/7 is mediated by a direct repeat

186 presence of genes arranged in tandem arrays, trans-splicing of messenger RNAs, and a reduced role for

187 of the last 5' intron, TSA and TSB, promote trans-splicing of mod(mdg4).

188 Trans-splicing of one of two short leader RNAs, SL1 or S

189 In T. brucei, spliced leader (SL)-mediated trans-splicing of pre-mRNAs is an obligatory step in gen

190 chimeric gene products normally generated by trans-splicing of RNAs in developing tissues.

191 the existence of chimeric RNAs generated by trans-splicing of RNAs transcribed from distant genomic

192 In kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic p

193 mation of the upstream mRNA and SL2-specific trans-splicing of the downstream mRNA.

194 n of mouse derived peptides, suggesting that trans-splicing of the knock-in human cDNA with the endog

195 We show that EMB2654 is required for the trans-splicing of the plastid rps12 transcript and that

196 t (RNA maturation of psaA 7) is deficient in trans-splicing of the second intron of psaA, and may be

197 of a multimeric complex that is required for trans-splicing of the second intron of psaA.

198 Therefore, trans-splicing of the SL1 leader sequence may serve at l

199 I intron ribozymes have been used to mediate trans-splicing of therapeutically relevant RNA transcrip

200 Trans-splicing of trypanosomatid polycistronic transcrip

201 ons, compensatory changes in U1 snRNA rescue trans-splicing of TSA mutants, demonstrating that U1 rec

202 idine did not increase the rate or extent of trans-splicing or cleavage under typical assay condition

203 elations between stage-specific preferential trans-splicing or polyadenylation sites and differential

204 r gamma or mutant beta-globin gene addition, trans-splicing or RNA interference.

205 Coupled with experimental examination of trans-splicing patterns, our comparative genomic analysi

206 ggest that operons and "spliced leader" (SL) trans-splicing predate the radiation of the nematode phy

207 NA-seq by taking advantage of the endogenous trans-splicing process.

208 The 'trans-splicing' process in lower eukaryotes is well unde

209 olypeptides through a process called protein trans splicing (PTS).

210 The protein trans-splicing (PTS) activity of naturally split inteins

211 Expressed protein ligation (EPL) and protein trans-splicing (PTS) are both intein-based approaches th

212 Protein trans-splicing (PTS) by split inteins has found widespre

213 olypeptides through a process termed protein trans-splicing (PTS).

214 ications on cellular chromatin using protein trans-splicing (PTS).

215 he first nematode to demonstrate a higher SL trans-splicing rate using a species-specific SL over wel

216 ith serine decreased N-terminal cleavage and trans-splicing rates; however, this substitution did not

217 nes have 5' ends, they receive their caps by trans splicing rather than by capping enzymes.

218 SMaRT creates a hybrid mRNA through a trans-splicing reaction between an endogenous target pre

219 ell-to-cell variability in ribozyme-mediated trans-splicing reaction efficiency.

220 l be applicable in any organisms that have a trans-splicing reaction from spliced leader RNA.

221 This trans-splicing reaction has ATP, Mg(2+), and splice-site

222 he ACT1-CUP1 reporter gene, resulting from a trans-splicing reaction in which a 5' splice site-like s

223 The trans-splicing reaction involves the association of 5' a

224 fragments in the hinge region by the protein trans-splicing reaction of a split intein to generate Bs

225 conclusion from this observation is that the trans-splicing reaction that takes place between transcr

226 ng these building blocks initiates an intein trans-splicing reaction that yields a hydrogel that is h

227 ranscription units that can be combined in a trans-splicing reaction to form the mature Mod(mdg4)2.2-

228 The trans-splicing reaction was partially blocked at a conce

229 Efficiency and specificity of the trans-splicing reaction were found to vary depending on

230 genic plants for reconstitution of a protein trans-splicing reaction.

231 are required for at least two stages of the trans-splicing reaction.

232 ent of SL ribonucleoproteins consumed during trans-splicing reactions is essential for effective tran

233 cubation of complementary pairs, we observed trans-splicing reactions with unprecedented rates and yi

234 ssembly of three distinct precursor RNAs via trans-splicing reactions, and the accumulation of nad5T1

235 ut suggest that apparent exon scrambling and trans-splicing result, respectively, from in vitro artif

236 Tau RNA trans-splicing resulted in an increase in exon 10 inclus

237 Trans-splicing ribozymes are RNA-based catalysts capable

238 established to select efficient and specific trans-splicing ribozymes from a ribozyme library.

239 Based on this ability of trans-splicing ribozymes to deliver new gene activities,

240 Trans-splicing ribozymes were used to splice sequences e

241 tem that involved the coexpression of a SMN2 trans-splicing RNA and an antisense RNA that blocks a do

242 ween an endogenous target pre-mRNA and a pre-trans-splicing RNA molecule (PTM).

243 Here, we evaluated the potential of trans-splicing RNA reprogramming to correct tau mis-spli

244 In SL trans-splicing, SL-donor transcripts (SL RNAs) contain t

245 Spliced leader trans-splicing (SLTS) plays a part in the maturation of

246 reprogramming using spliceosome-mediated RNA trans-splicing (SMaRT) could be a method of choice to co

247 Spliceosome-mediated RNA trans-splicing (SMaRT) provides an effective means to re

248 We carried out spliceosome-mediated RNA trans-splicing (SMaRT) to repair mutant FVIII mRNA.

249 uld be modulated by spliceosome-mediated RNA trans-splicing (SMaRT).

250 RNP proteins function by participating in a trans-splicing specific network of protein protein inter

251 anscription of SLRNA genes, which encode the trans splicing-specific spliced leader RNA, suggests tha

252 The nematode SL is derived from a trans-splicing-specific approximately 100-nucleotide RNA

253 within the polycistronic pre-mRNA, and that trans-splicing specificity is recapitulated in vitro.

254 by a gene from another organism, no loss of trans-splicing specificity was observed, suggesting that

255 We examined the cis- vs. trans-splicing status of the mitochondrial group II intr

256 Here, we addressed this issue using a trans-splicing strategy that allows modulating tau exon

257 We employed pre-mRNA segmental trans-splicing (STS), in which two engineered DNA fragme

258 ated synthesis of spliced leader (SL) RNA, a trans splicing substrate and key molecule in trypanosome

259 The SL RNA is a small nuclear RNA and a trans splicing substrate for the maturation of all pre-m

260 his information was used to develop a tandem trans-splicing system based on native and engineered spl

261 This plasmid-based trans-splicing system is adaptable to multiple gene deli

262 we reported the development of an optimized trans-splicing system that involved the coexpression of

263 We demonstrate that the RNA trans-splicing technology combined with a SIN lentiviral

264 replaces the mutant-containing 3' portion by trans-splicing the corresponding 4-kb wild-type sequence

265 We show that due to alternative trans splicing, the human pathogen Leishmania naturally

266 the joining mechanism possibly involves tRNA trans-splicing, the presence of an intron might have bee

267 We define an RNA sequence required for SL2 trans-splicing, the U-rich (Ur) element, through mutatio

268 Although it is sometimes assumed to arise by trans-splicing, there is no evidence of this and the eff

269 spliced leader RNA and joined to pre-mRNA by trans-splicing, thus providing mature mRNAs with an m7G

270 tion was also determined to be necessary for trans splicing to proceed on a substrate that had U2 snR

271 h Zn(2+), reactants can be diverted from the trans splicing to the trans cleavage pathway where DTT a

272 Finally, increased use of SL1 trans-splicing to downstream operon genes can indicate t

273 ammable DNA-binding protein by using protein trans-splicing to ligate synthetic elements to a nucleas

274 non-covalent fragments of the ribozyme allow trans-splicing to occur in both a reverse splicing assay

275 ugh the cap 4 modifications are required for trans-splicing to occur, the underlying mechanism remain

276 t transgenic vectors, and rejoin via protein trans-splicing to reconstitute a full-length marker prot

277 These findings add trans-splicing to the list of RNA-related functions asso

278 f nascent transcripts by polyadenylation and trans-splicing, together with specific rates of mRNA tur

279 agment, supports rapid and efficient protein trans-splicing under a range of conditions, enabling sem

280 spliced to both, indicating that SL1 and SL2 trans-splicing use different underlying mechanisms.

281 ternative splicing, and it differed from the trans splicing used in nematode operons.

282 Protein trans-splicing using split inteins is well established a

283 This occurs by 3' end formation and trans-splicing using the specialized SL2 small nuclear r

284 trate that in vivo delivery of the optimized trans-splicing vector increases an important SMN-depende

285 were compared to homologous AV5:5 and AV2:2 trans-splicing vector sets for their ability to reconsti

286 e splitting site has a profound influence on trans-splicing vector-mediated gene expression.

287 to-head fashion, we generated two hybrid ITR trans-splicing vectors (AV5:2LacZdonor and AV2:5LacZacce

288 as a guide, we generated a set of efficient trans-splicing vectors and achieved widespread expressio

289 Unfortunately, the efficiency of current trans-splicing vectors is very low.

290 This demonstrates the use of trans-splicing vectors to efficiently express a large th

291 These hybrid trans-splicing vectors were compared to homologous AV5:5

292 Since defective trans-splicing was detected as early as 5 min after inhi

293 Specific trans-splicing was observed in living animals (P = 0.086

294 In searching for examples of trans-splicing, we looked only at reproducible events wh

295 -terminal cleavage, C-terminal cleavage, and trans-splicing were (1.0 +/- 0.5) x 10(-3), (1.9 +/- 0.9

296 ivation energies for N-terminal cleavage and trans-splicing were determined by Arrhenius plots to be

297 species and is essential and sufficient for trans-splicing, which binds U1 small nuclear RNP (snRNP)

298 an antibody Fc fragment by N- and C-terminal trans-splicing with short peptide tags (CysTag) that con

299 liced pre-mRNAs and the disappearance of the trans-splicing Y structure intermediate, CRK9 is essenti

300 ressed does the split intein mediate protein trans-splicing, yielding a full-length T7 RNA polymerase