ライフサイエンスコーパス: transfection

1 hat lead to high toxicity and/or ineffective transfection.

2 around 15-20% ITCH silencing 48 hours after transfection.

3 n of Sema3G levels in the hippocampus by AAV transfection.

4 ently inducing BBB opening and extravascular transfection.

5 and down-regulated, respectively, 72 h after transfection.

6 clinic remain, such as the need for genetic transfection.

7 han their younger N6W counterparts after HBV transfection.

8 epolymerization and apoptosis, and cell line transfection.

9 ed down by siRNA or overexpressed by plasmid transfection.

10 caveats, including a heavy reliance on viral transfection.

11 Similar effects were observed with ANP-siRNA transfection.

12 ue to -OH group is required to maintain high transfection.

13 hat are particularly potent in T lymphocytes transfection.

14 DNA thereby providing an alternative to DNA transfection.

15 targeting of all seven PHY genes in a single transfection.

16 but they failed to do so in 80 to 90% of the transfections.

17 One-pot evaluation enabled by poly-transfection accelerates and simplifies the design of ge

18 We first benchmark poly-transfection against co-transfection, showing that titra

19 feature is in great contrast to a commercial transfection agent with similar transfection performance

20 f homing to nuclei of cultured cells without transfection agents or viral transduction.

21 livery of macromolecules because many of the transfection agents that are commonly used with animal c

22 as co-delivering immobilized siRNAs without transfection agents.

23 and down-regulated, respectively, 48 h after transfection and 177 and 172 up- and down-regulated, res

24 iol (2-MeOE2), small interfering RNA (siRNA) transfection and adenovirus overexpression were used.

25 smid DNA in the cytosol within 15 minutes of transfection and at consistent levels for 4 h.

26 By means of siRNA-mediated transfection and electron microscopy we showed that mode

27 which enable both robust virus production by transfection and evaluation of DTA-expressing virus infe

28 (siRNA) duplexes, SNAs act as single-entity transfection and gene silencing agents and have been use

29 properties of desmin were addressed by cell transfection and in vitro assembly experiments.

30 Y102E in both transfection and infection conditions was unable to main

31 reporter gene was detected a few hours after transfection and persisted for 3 days in cells transfect

32 Protoplast transfection and regeneration systems are useful platfor

33 Transfection and silencing were performed in Caco2 cells

34 by the GR antagonist RU-486 and by GR siRNA transfection and that DPP4 enzyme activity is reduced by

35 phytosterols into LNPs (eLNPs) enhances gene transfection and the length of alkyl tail, flexibility o

36 elivery vehicles for saRNA both for in vitro transfections and in vivo immunogenicity and generate ce

37 Cell transfections and primary T-cell assays were used to eva

38 berculosis infection, cytosolic nucleic acid transfection, and IFN-beta treatment.

39 tensity electric fields is a simple means of transfection applicable to most cell types.

40 , but that overall trends in the location of transfection are not affected by injury.

41 We introduce nanostraw-assisted transfection as an alternative method for RNA delivery t

42 Here, using a transient-transfection assay, residues 80 to 130 of the ectodomain

43 Co-transfection assays (Col3a1 promoter-luciferase reporter

44 precipitations and luciferase reporter-based transfection assays, CHOP was found to up-regulate SERPI

45 ases in conjunction with mouse gonad explant transfection assays, we identified TCF/LEF-binding seque

46 These differences were recapitulated in CD4 transfection assays, which revealed a strong association

47 examined, unlike their behavior in transient transfection assays.

48 ized sites were established by reporter gene transfection assays.

49 , which we validated with ChIP and transient transfection assays.

50 ubstitutions (RASs) were tested in transient-transfection assays.

51 cape which is not achievable using exogenous transfection-based luciferase reporter assays.

52 miR-200b-3p mimic transfection before TGF-beta1 stimulation maintained epi

53 The perpendicular pathway also leads to transfection, but release is slower.

54 , in part because the extent to which pooled transfections can be performed in these species remains

55 dly, PMA treatment, but not APOBEC3A plasmid transfection, caused a cessation in cell growth.

56 1 subunit silencing by small interfering RNA transfection changed the GABA-response characteristics i

57 dation of the outer ZN matrix and release of transfection competent CS/DNA NPs occurred in simulated

58 We test the method on a variety of miRNA transfection datasets and demonstrate increased accuracy

59 miR-143 and miR-145 transfection decreased cervical cell number by increasin

60 These lipidoids showed comparable transfection efficacies but much lower cytotoxicities co

61 ried PBAE NP formulations were evaluated for transfection efficacy and cytotoxicity to a range of hum

62 P release profile, in vitro cytotoxicity and transfection efficacy in Hek 293 cells were characterize

63 ether overexpression of NC1 domain with high transfection efficacy would perturb spermatogenesis, in

64 vo is technically challenging due to the low transfection efficacy.

65 reen fluorescent protein (EGFP) and assessed transfection efficiencies by quantifying levels of EGFP

66 mRNA/Ser-CART transfection efficiencies of >95% are achieved in vitro.

67 aematopoietic progenitor CD34(+) cells, with transfection efficiencies of 46.3 +/- 5.2%, 23.0 +/- 2.1

68 In vitro transfection efficiencies of these complexes were determ

69 We achieved high transfection efficiency (63.6 +/- 3.44% EGFP + viable ce

70 Cell viability and electro-transfection efficiency (eTE) are dependent on various e

71 ically optimized for CSCs yielding 45.77% of transfection efficiency and 89.75% of viability.

72 e TLR agonists R848 and 3M-052, for in vitro transfection efficiency and cytotoxicity.

73 heir use in gene delivery often exhibits low transfection efficiency and stability.

74 inst intracellular targets involve their low transfection efficiency and suitable reversible encapsul

75 n 4 (G4) with Adv (G4/Adv) to strengthen its transfection efficiency and then loaded G4/Adv into a bi

76 and flow cytometry analyses showed efficient transfection efficiency and uniform distribution of PLGA

77 to be cell line specific, COS7 showed higher transfection efficiency compared to SH-SY5Y.

78 arantee the effectiveness: 2 folds or higher transfection efficiency enhancement and rapid transgene

79 cantly improved cellular internalization and transfection efficiency in macrophages, depending on the

80 ationic liposomes resulted in an increase in transfection efficiency in mammalian cell lines.

81 The transfection efficiency in vitro increased significantly

82 trate brain extracellular matrix to increase transfection efficiency in vivo.

83 stem cells (ADSCs) and astrocytes, and high transfection efficiency is achieved (77% in human ADSCs

84 tment of HMEC-1 cells at N:P 6 resulted in a transfection efficiency of 33.7%, negligible cytotoxicit

85 study has investigated the encapsulation and transfection efficiency of cationic liposomes prepared f

86 o P. falciparum, we observed that the higher transfection efficiency of P. knowlesi resulted in near

87 ection, suggesting that the NP-mediated mRNA transfection efficiency was consistent with the endocyto

88 ary human T cells and demonstrated up to 95% transfection efficiency with minimum impact on cell viab

89 injection directly impacts the magnitude of transfection efficiency, but that overall trends in the

90 ome formulations showed the highest in vitro transfection efficiency, the fluid and solid phase lipos

91 Sucrose didn't affect the transfection efficiency, while sorbitol resulted in a fl

92 is a frequent event despite the inefficient transfection efficiency.

93 ol resulted in a fluctuation of the in vitro transfection efficiency.

94 s of human mast cell cultures and their poor transfection efficiency.

95 ar to destabilise the wildtype protein in co-transfection experiments in a human RPE cell line.

96 Finally, in transfection experiments, efficient protein expression f

97 , fully leveraging the single-cell nature of transfection experiments.

98 At the level of transfection, fibers were present in the inner molecular

99 UB-GFP plasmids and for at least 5 days post-transfection for cells transfected with pEYFP-Mitotrap.

100 ceptor tyrosine kinase RET (rarranged during transfection) gene alterations have been identified as o

101 K562 transfections generated stable cell lines running for 28

102 The superior drug delivery/gene transfection/genome-editing efficiencies of the SMOF NP

103 Acoustic-transfection has advantages over typical sonoporation be

104 ted NLRP3 inflammasome activation during LPS transfection; however, its role in Gram-negative bacteri

105 erum, they produced transformed foci without transfection in 10% CS, but not in 2% CS.

106 Isolation of cells and their transfection in a controlled manner is an integral step

107 had increased promoter activity on transient transfection in H295R cells compared with WT intron 2.

108 CHIP transfection in HIV-1 reporter TZM-bl cells resulted in

109 Using transient transfection in Kc167 cells, we systematically analyze t

110 reporter genes to evaluate NP-mediated gene transfection in macrophages.

111 bellum via adenoassociated virus (AAV) viral transfection in mice, the mutant BK(G354S) channel, but

112 The approach afforded efficient transfection in primary human fibroblasts as well as mes

113 s showed the highest amount of reporter gene transfection in the retina.

114 with CS/DNA NP cores successfully mediating transfection in vitro.

115 groups are required for polyplex uptake and transfection in vitro.

116 Transient co-transfections in Arabidopsis protoplasts showed that A-Z

117 In vitro treatment with let-7 mimic transfections in human myofibroblasts reduced ERalpha ex

118 tion of Drp1 by Mdivi-1 treatment or si-Drp1 transfection increased CDDP sensitivity of ovarian cance

119 but not all peptides resulted in successful transfection; indicating the influence of peptide design

120 ein, by spironolactone treatment or by siRNA transfection, inhibits SM-dependent late lytic gene tran

121 vide visual confirmation of successful shRNA transfection inside embryos through electroporation.

122 as quantified using flow cytometry following transfection into an immortalized T-cell line and compar

123 al inflammasome, and that lipopolysaccharide transfection into the host cytoplasm is sufficient to tr

124 Our results show that nanostraw-mediated transfection is a gentle alternative to established gene

125 Transfection is a key function for many single-cell anal

126 However, at early time points after transfection, its action appears to be inhibitory.

127 Moreover, using single-axon transfection labeling, we demonstrate that the above dif

128 anti-miR-34a or -449a or through PACS-1 cDNA transfection led to the reversal of DNA damage response

129 ion methods and revealed that schizont-stage transfection limited the tendency for parasites to take

130 sion of the reporter proteins with transient transfection, live cell image acquisition with synchroni

131 e the first reported intracerebroventricular transfections mediated by polymeric gene carriers in a m

132 However, in vivo transient and stable transfection method has not been established for Babesia

133 We present a 'poly-transfection' method as a simple yet high-throughput alt

134 t reporters allowed us to evaluate different transfection methods and revealed that schizont-stage tr

135 Here we used a mosaic transfection model to create myotubes that contained exa

136 Using DUSP4 transfection models and genetically modified CREM-defici

137 scavenger receptors that facilitate cellular transfection, nucleic acid-metal organic framework nanop

138 ase L-dependent cell death were increased by transfection of 2-5A, by deficiencies in ADAR1 (which ed

139 orescence staining of endogenous SLC26A9 and transfection of a 3HA-tagged construct into well-differe

140 Transfection of a dominant-negative STAT3 mutant, but no

141 In human embryonic kidney HEK293 cells, the transfection of a duplex HBoV1 genome initiates viral DN

142 Transfection of a NME1 site-directed mutant lacking hist

143 viable method for highly efficient non-viral transfection of ADSCs and astrocytes.

144 Furthermore, transfection of an mRNA construct that expresses VEGF-C

145 Transfection of Ash2l with W118A mutation to disrupt Ash

146 Furthermore, direct transfection of bone with VEGF RNA in vivo increased int

147 Silencing EGFL6 mRNA by shRNA transfection of cancer cells also significantly reduced

148 Transfection of cell lines with ZNHIT3 expression vector

149 For transfection of cells to incorporate exogenous materials

150 Transfection of cells with full-length CaM slightly incr

151 Transfection of cells with plasmid carrying mutations in

152 Following transfection of cells with UnaG and infection with wild-

153 Conversely, transfection of Chinese hamster ovary cells with the cor

154 In addition, transfection of chromatin results in negligible cytotoxi

155 Transfection of ClC-6(Tyr553Cys), but not ClC-6(WT), gen

156 Indeed, transfection of CLL cells with Wnt5a small interfering R

157 ilar to those of wild-type viruses following transfection of cultured cells.

158 Additionally, co-transfection of D. melanogaster S2 cells with dual lucif

159 h CRISPR/Cas9-mediated inactivation of Dlg1, transfection of Dlg1 enhances beta-catenin signaling ~4

160 Consistently, the transfection of each miRNA impeded cell migration.

161 Transfection of eight upregulated LTRs into recipient hu

162 Transfection of either ClC-6(Tyr553Cys/Glu200Ala) or ClC

163 Transfection of full-length HLA-DPB1 mRNA into knockout

164 Transfection of healthy monocytes with miR-155 induced a

165 Moreover, transfection of healthy non-carrier sibling OPCs confirm

166 validated through trapping and simultaneous transfection of HEK-293 embryonic kidney cells with a pl

167 Furthermore, transfection of HEK293 cells with SLC43A3 increased the

168 fficiencies in transformation of E. coli and transfection of HEK293T cells.

169 This included viral transfection of hippocampal interneurons with channelrho

170 or slower and more sustained (solid bioinks) transfection of host or transplanted cells in vivo.

171 Potent transfection of human corneal epithelial cells with siRN

172 Transient transfection of human embryonic kidney 293 cells with th

173 tional suppressor, we overexpressed BC200 by transfection of in vitro transcribed RNA and transient e

174 Transfection of K562 cells with miR-9-3p or miR-9-5p mim

175 s through transfection of wild-type PPIP5K1; transfection of kinase-dead PPIP5K1 was ineffective.

176 In vivo adenoviral transfection of KLF2 to the carotid bodies in CHF rabbit

177 Stable re-transfection of knockout podocytes with inducible APOL1-

178 Transfection of LNAA to VZVsncRNA14 and VZVsncRNA9 decre

179 Active cell targeting and magnetic transfection of lung carcinoma cells are demonstrated us

180 Transfection of MCs with mimic or inhibitor of miR-4443

181 the reduction of AQP5 mRNA expression by the transfection of miR-1226-3p and a luciferase reporter as

182 We recapitulated these results with direct transfection of miR-16-5p, confirmed that this is a canc

183 the reduction of AQP5 translation after the transfection of miR-19b-3p in MDA-MB-231 cells.

184 Transfection of miR-3085-3p into chondrocytes decreases

185 Conversely, transfection of miR-9-3p or miR-9-5p inhibitors in K/VP.

186 The transfection of miRNA mimics, cell cycle distribution an

187 ts are observed in mammalian cells following transfection of mRNA encoding five of these next-generat

188 Following transfection of MSCs with the mms6 gene there is bio-ass

189 siRNA transfection of murine Zranb3 in MIN6 beta-cells results

190 Transfection of p44/42 MAPK or hnRNP F small interfering

191 Co-transfection of PCR cassettes with a Cas12a-encoding pla

192 Additionally, transfection of peripheral blood CLL cells with STAT3 sh

193 h the use of phospho-specific antibodies and transfection of phospho-inactive DEPDC5 mutants.

194 transgenesis in quail, based on the in vivo transfection of plasmids in circulating Primordial Germ

195 Transfection of polarized RPE (porcine primary cells, iP

196 iant translocation 1 (PVT1) was inhibited by transfection of primary ASMCs with small interfering RNA

197 rization of fast iGluSnFR variants in vitro, transfection of pyramidal cells in organotypic hippocamp

198 5 induced a decrease in M2-like markers, and transfection of RA monocytes with antagomir-155 allowed

199 Surprisingly, 5 days after the transfection of Rab5B siRNA, HBV DNA in the supernatant

200 several proposed cytosolic DNA sensors after transfection of several tumor cell types with plasmid DN

201 lls that had been depleted of GADD45gamma by transfection of short hairpin RNA (shRNA) or in which th

202 Our electroporation protocol allows for the transfection of shRNAs into hundreds of fertilized H. sy

203 Transfection of Sirtuin-1 small interfering RNA prevente

204 Transfection of small interfering (si)-TTP RNA upregulat

205 We show that a single transfection of small interfering RNA targeting class II

206 Transfection of small interfering RNAs targeting YAP/TAZ

207 Transfection of such motifs from Meg3 RNA, termed triple

208 Co-transfection of Tat with a CHIP-expressing plasmid decre

209 Notably, transfection of the affected lymphocytes with MAGT1 mRNA

210 sent a method of transient as well as stable transfection of the Babesia microti (B. microti) in the

211 US method that elicits endothelial-selective transfection of the cerebral vasculature (i.e., "sonosel

212 We observed heterogeneous, low-level transfection of the collecting duct, proximal tubule, di

213 Transfection of the mutant GRTH construct in COS-1 cells

214 Transient transfection of the p.G334R allele conferred a mild defe

215 Transfection of the variant ADAMTS17 into HEK293T cells

216 Transfection of these cell lines with ITCH siRNA could e

217 Transfection of three spectrally-distinct fluorescent re

218 2)S treatment reversed the changes above and transfection of triple genes producing H(2)S (CBS, CSE a

219 or stably-expressed TDP2-targeted shRNA and transfection of various TDP2 mutants to show that its ex

220 Transfection of viral RNA into primary human cardiomyocy

221 The MACs are constructed by transfection of vitamin C lipid nanoparticles that deliv

222 ued by restoration of InsP(8) levels through transfection of wild-type PPIP5K1; transfection of kinas

223 Using transfections of 293A cells and Western blotting experim

224 al approach to perform DNA, RNA, and protein transfections of cells.

225 romoter from nucleotides (nt) -318 to +54 by transfections of luciferase reports containing different

226 ly downscaled an 8-d protocol based on daily transfections of mRNA encoding for reprogramming factors

227 rghei and Toxoplasma gondii, in which pooled transfections of multiple vectors were critical to incre

228 ated covalent heterodimeric proteins upon co-transfections of plasmids encoding separate proteins and

229 Here we report that consecutive transfections of two sets of synthetic mRNAs encoding tr

230 l membrane models show two distinct modes of transfection: parallel and perpendicular.

231 this assay, we optimize various base editing transfection parameters and delivery strategies.

232 a commercial transfection agent with similar transfection performance but poor protection capability

233 We describe novel CRISPR/Cas9 transfection plasmids and approaches for the speedy, sta

234 t AID) using either a one-step or a two-step transfection platform to obtain triply mutated and/or ed

235 pecific inhibitor of NQO1 and siRNA and gene transfection procedures unambiguously demonstrated NQO1

236 down of Notch3 expression by transient siRNA transfection promoted the expression of osteogenic trans

237 Here, we devise an improved transfection protocol of in vitro transcribed Cas9 mRNA

238 of partially edited regenerants (a two-step transfection protocol) allowed us to derive ETR1 fully e

239 about 14), which were associated with a high transfection rate and low toxicity.

240 rus promoter was complexed with a commercial transfection reagent and administrated using different r

241 Neutrals (siRNNs) that enter cells without a transfection reagent are used to target Plk1 mRNA in pri

242 uptake could be modulated by using a protein transfection reagent Xfect.

243 culation of the rZIKV cDNA in the absence of transfection reagent.

244 obes that can enter cells without the aid of transfection reagents and can detect, track, and quantif

245 -generation cEt-ASOs can enter cells without transfection reagents.

246 Rab5-GTP loading, as shown by knockdown and transfection recovery experiments.

247 Many methods of cell transfection rely on unspecific means to increase the tr

248 Each cell in a poly-transfection represents an independent measurement at a

249 roto-oncogene 1 (ROS1) and rearranged during transfection (RET)) has established an oncogene-centric

250 richment strategies that employ reporters of transfection (RoT), TREE significantly improved the edit

251 enetic systems in a single, readily-prepared transfection sample.

252 first benchmark poly-transfection against co-transfection, showing that titration curves for commonly

253 GluN2D(Ala678Asp) transfection significantly decreased cell viability of r

254 relatively more effective in rats with viral transfection spaces situated lateral to the DA depletion

255 em cells for both two- and three-dimensional transfection strategies.

256 Cas9-linked selection marker and a staggered transfection strategy promotes targeting efficiency up t

257 In vitro transfection studies and gene ontology revealed involvem

258 racted with B5 as predicted by the transient-transfection studies but had a small-plaque phenotype.

259 Cell transfection studies disclosed a dual behavior of R406W-

260 Transfection studies suggest that inactive caspase-8 ado

261 Using bioinformatic analysis and co-transfection studies we further identified the EGR1 tran

262 of miRs in MDSC activation was confirmed by transfection studies.

263 phages also demonstrated more efficient mRNA transfection, suggesting that the NP-mediated mRNA trans

264 Using a novel transfection system in which HDV replication is initiate

265 ells, providing the evidence of the acoustic-transfection technique for precise genome editing using

266 Stable transfection technique has been reported in many protozo

267 e polyadenylation signal, a robust transient transfection technique was established for the first tim

268 and local intracellular delivery to acoustic-transfection technique.

269 igate the anatomical parameters that dictate transfection through this route of administration.

270 Finally, we use poly-transfection to rapidly engineer a difficult-to-optimize

271 We then use poly-transfections to efficiently generate new insights, for

272 The transfection using an electroporation method and genetic

273 controlled electroporation with the goal of transfection using human embryonic kidney cells (HEK-293

274 s over typical sonoporation because acoustic-transfection utilizing ultra-high frequency ultrasound o

275 Acoustic-transfection was further demonstrated to deliver CRISPR-

276 Finally, in vitro lentiviral transfection was used to induce sustained VDR-KD in C2C1

277 terodimerization in mammalian cell transient transfections was observed, but levels were influenced b

278 Using 3D electron microscopy and single-cell transfection, we investigated the mouse Posterior thalam

279 After transfection, we show increased levels of the full-lengt

280 Using both luciferase and GFP plasmid transfections, we show that the time delay between injur

281 mall hairpin RNA interference and adenovirus transfection were adopted to manipulate the expressions

282 biochemical and functional approaches, cell transfection, Western blot, confocal microscopy, cell de

283 motion require potentially cytotoxic dyes or transfection, which can alter cellular behavior and are

284 terplay between microRNA-126 and HIF-1alpha, transfection with a microRNA-126 inhibitor downregulated

285 tion of NADH, reduced expression of CtBP, or transfection with an NAD(H) insensitive CtBP, and are re

286 nitiation, we show that in HEK-293 cells, co-transfection with both Homer1c and CaSR, but neither wit

287 Transfection with CTMP siRNA significantly increased Akt

288 of the cationic end blocks led to efficient transfection with DDNDs.

289 atment with the FOXO1 inhibitor AS1842856 or transfection with FOXO1-specific siRNAs protected agains

290 A combination of roscovitine treatment, transfection with immediate early genes (IE), and infect

291 Transfection with increasing amounts of WT-CFTR cDNA pro

292 Cell transfection with miR-223 mimic or antagomiR proved the

293 We demonstrate transfection with muVS by delivery of a 996-nucleotide m

294 We also demonstrated that transfection with the novel mutants conferred cells high

295 WT PIGK also rescued the GPI-AP levels, but transfection with the two tested mutant variants did not

296 oblasts, and this phenotype was corrected by transfection with the wild-type but not the mutated PAIC

297 of blood vessel and bone formation following transfection with VEGF RNA or delivery of recombinant hu

298 In contrast, transfection with VEGF RNA triggered angiogenic effects

299 In a knockout cell line, transfection with WT PIGK also rescued the GPI-AP levels

300 Transfections with a reporter plasmid containing the 3'

301 cerebral vasculature (i.e., "sonoselective" transfection), without opening the BBB.