ライフサイエンスコーパス: transfection efficiency

1 ed lipid displayed optimal peptide-targeted, transfection efficiency.

2 n (Fluc-eGFP) for noninvasive measurement of transfection efficiency.

3 output that is independent of cell number or transfection efficiency.

4 nthesized and evaluated for DNA delivery and transfection efficiency.

5 role in DNA delivery and in determining the transfection efficiency.

6 is a frequent event despite the inefficient transfection efficiency.

7 been proposed to be a major reason for poor transfection efficiency.

8 litating DNA release and leading to superior transfection efficiency.

9 -viral gene vectors from having an effective transfection efficiency.

10 dothelial cells and permitted calculation of transfection efficiency.

11 regulation of reporter genes or to normalize transfection efficiency.

12 that these could have significant effects on transfection efficiency.

13 r use has been limited by the relatively low transfection efficiency.

14 ectrophoresis and loss of transformation and transfection efficiency.

15 g in vivo cardiac gene transfer has been low transfection efficiency.

16 lutinating virus of Japan-liposome with >95% transfection efficiency.

17 atio to determine if either variable affects transfection efficiency.

18 es into immunocompetent mice without loss of transfection efficiency.

19 luciferase construct was used to control for transfection efficiency.

20 nactivated adenovirus significantly enhances transfection efficiency.

21 monstrated compared to controls despite a 1% transfection efficiency.

22 iposome, lipofectamine, further enhanced the transfection efficiency.

23 brane played a secondary role in determining transfection efficiency.

24 a suspension at 4 degrees C with no loss in transfection efficiency.

25 ake, reduced cytotoxicity, and improved gene transfection efficiency.

26 tions have been employed to siRNA for better transfection efficiency.

27 h substantially increases cell viability and transfection efficiency.

28 vestigated for gene delivery due to its high transfection efficiency.

29 heir intracellular delivery and overall gene transfection efficiency.

30 In contrast, hLNC-PEG significantly reduced transfection efficiency.

31 f CaCO(3) to PS significantly influences the transfection efficiency.

32 ution, cellular uptake, endosomal escape and transfection efficiency.

33 odegradability, synthetic accessibility, and transfection efficiency.

34 ol resulted in a fluctuation of the in vitro transfection efficiency.

35 and time-dependent manner, resulting in high transfection efficiency.

36 a 'double-shell' miRNA distribution and high transfection efficiency.

37 thus explaining the synergistic increase in transfection efficiency.

38 t cellular uptake and significantly enhanced transfection efficiency.

39 ficulties of nucleic acid packaging and poor transfection efficiency.

40 on, resulting in significantly improved gene transfection efficiency.

41 membrane rupture and therefore enhances the transfection efficiency.

42 s of human mast cell cultures and their poor transfection efficiency.

43 ative to electroporation with an increase in transfection efficiency.

44 morphology, epithelial barrier function and transfection efficiency.

45 ta potential, as well as cellular uptake and transfection efficiency.

46 eps might be manipulated in order to improve transfection efficiencies.

47 coming cellular barriers, resulting in lower transfection efficiencies.

48 ll retained their biophysical properties and transfection efficiencies.

49 rnary nitrogen centers) in modulating the DC-transfection efficiencies.

50 apy due to their multifunctionality and high transfection efficiencies.

51 escent intensity 85.3 [SD 48.5]; and average transfection efficiency 23.3% [range 10.6-30.9]).

52 (-s-s-) derivative (16 kDa) showed excellent transfection efficiency: 3.6 times higher than branched

53 We achieved high transfection efficiency (63.6 +/- 3.44% EGFP + viable ce

54 the following conditions appear to optimize transfection efficiency: a DNA:DOTAP ratio of 1:6; a 24

55 h Chol at a 1:1 molar ratio gave the highest transfection efficiency after intravenous administration

56 drug delivery mechanisms have increased the transfection efficiency aiding in greater therapeutic re

57 Ad5RGDpK7 exhibited higher transfection efficiency, allowing a significant reductio

58 nversion frequencies reaching 30-70% at high transfection efficiencies and approximately 2% at low tr

59 the use of only two plasmids, which enhances transfection efficiencies and hence vector production.

60 Transfection efficiencies and transgene expression kinet

61 Transfection efficiencies and VEGF inhibition in LNCaP a

62 ther, functionalized nanoparticles exhibited transfection efficiencies and VEGF inhibition significan

63 ically optimized for CSCs yielding 45.77% of transfection efficiency and 89.75% of viability.

64 r in vivo gene therapy due to their targeted transfection efficiency and ability to penetrate tissues

65 te an increase of 3-5 orders of magnitude in transfection efficiency and are potent activators of den

66 vivo delivery without compromising in vitro transfection efficiency and are thus promising carriers

67 Comparative analyses of transfection efficiency and cell viability for primary a

68 ndomly distributed cells, leading to limited transfection efficiency and cell viability, especially f

69 Despite superior transfection efficiency and cell viability, high cost of

70 addresses a key bottleneck in balancing the transfection efficiency and cell viability, which can be

71 hniques face a fundamental trade-off between transfection efficiency and cell viability; achieving bo

72 Transfection efficiency and cellular internalization of

73 However, transfection efficiency and cytotoxicity vary widely bet

74 thods, we screened this library in vitro for transfection efficiency and cytotoxicity.

75 e TLR agonists R848 and 3M-052, for in vitro transfection efficiency and cytotoxicity.

76 a reporter gene show no correlation between transfection efficiency and cytotoxicity.

77 MAM dendrimer complexes can be used for high transfection efficiency and effective targeting of APCs

78 ize as a potential limiting factor affecting transfection efficiency and hence the influenza viral yi

79 This heterogeneity contributes to the low transfection efficiency and instability of cationic lipi

80 composition of acetals showed high in vitro transfection efficiency and low cytotoxicity in the deli

81 PEI (-s-s-) polyplexes showed higher transfection efficiency and lower cytotoxicity compared

82 outcomes depend on a proper balance between transfection efficiency and polyplex-induced cytotoxicit

83 eased incubation time up to 45 min increased transfection efficiency and reduced RI, but longer incub

84 d mRNA therapeutics has been limited by poor transfection efficiency and risk of vehicle-induced path

85 hed structures can significantly improve the transfection efficiency and safety of PAEs highlighting

86 revealed cell line-dependent differences in transfection efficiency and showed in-house cationic lip

87 heir use in gene delivery often exhibits low transfection efficiency and stability.

88 inst intracellular targets involve their low transfection efficiency and suitable reversible encapsul

89 postulate that the relationship between the transfection efficiency and the ac frequency is determin

90 a demonstrate a correlation between in vitro transfection efficiency and the combination of several p

91 ted alkaline phosphatase gene to control for transfection efficiency and the effects of culture condi

92 sable lipids (MC3 and C12-200) on stability, transfection efficiency and the inflammation and immunog

93 n 4 (G4) with Adv (G4/Adv) to strengthen its transfection efficiency and then loaded G4/Adv into a bi

94 Transfection efficiency and toxicity of PEI are highly d

95 shown to have an important influence on the transfection efficiency and toxicity of the polyplexes.

96 ncubation time, and AS-ODN concentrations on transfection efficiency and toxicity.

97 produce bioadhesive NPs (BNPs) with superior transfection efficiency and tropism for tumor cells.

98 and flow cytometry analyses showed efficient transfection efficiency and uniform distribution of PLGA

99 lthough the cellular uptake was similar, the transfection efficiency and VEGF expression of PAM-ABP u

100 scale devices suffer from the unsatisfactory transfection efficiency and/or cell viability due to the

101 owed efficient transfection of plasmids (40% transfection efficiency) and short interfering RNA (inte

102 on methods are available, cell toxicity, low transfection efficiency, and high cost remain hurdles fo

103 bility, superoxide production, cytotoxic RNA transfection efficiency, and measurement of SOD2 protein

104 val of transfected viral DNA was measured as transfection efficiency, and mutagenesis at the lesion s

105 Survival effects were determined from transfection efficiency, and mutation fixation at the le

106 Survival of the M13 DNA was measured as transfection efficiency, and mutation fixation at the le

107 carriers with low cytotoxicity and high gene transfection efficiency, and preferentially with the sel

108 c responses of signal transduction pathways, transfection efficiency, and subcellular localization of

109 initely, can be concentrated with no loss in transfection efficiency, and the toxicity levels can be

110 Targeted gene delivery, transfection efficiency, and toxicity concerns remain a

111 quency of up to 11.9% and when corrected for transfection efficiency approached 43%.

112 The PA:LFn-GAL4:ASO complexes had transfection efficiency approximately equivalent to Nucl

113 ugh hLNC-Hist did not achieve quite the peak transfection efficiency as LNPs in some models, hLNC-His

114 the importance of directly measuring in vivo transfection efficiency as the key barcoded parameter in

115 Transfection efficiency, as measured by luciferase prote

116 mplexation efficiency, release profiles, and transfection efficiency, as well as investigating mucoad

117 Their transfection efficiency, as well as that of the lamellar

118 rticle tracking microscopy, and quantitative transfection-efficiency assays on live cells to unveil t

119 zed defect in gene expression nor an altered transfection efficiency, because the islet amyloid polyp

120 However, uncertain transfection efficiency becomes a bottleneck, especially

121 injection directly impacts the magnitude of transfection efficiency, but that overall trends in the

122 reen fluorescent protein (EGFP) and assessed transfection efficiencies by quantifying levels of EGFP

123 hanically tailored complex which may enhance transfection efficiency by controlling the stability of

124 We have improved the transfection efficiency by means of recombinant adenovir

125 Transfection efficiency by PEG-modified, cleavable RTNs

126 The in vivo enhancement of transfection efficiency by this modified gene delivery v

127 x concentration at the cell surface enhanced transfection efficiency by up to 8.5-fold over the best

128 ediated DNA double-strand breaks, increasing transfection efficiency by ~400-fold to 1 transfectant p

129 ace composition on vector immobilization and transfection efficiency can also be studied.

130 ion potential, primarily aiming at increased transfection efficiency, cell selectivity and reduced cy

131 hat targeted and stabilized particles retain transfection efficiencies comparable to the nonstabilize

132 ce-specific knockdown in H3122 cells, with a transfection efficiency comparable to commercial polyeth

133 Our new gene carrier exhibits high gene transfection efficiency, comparable to or even better th

134 ble to reducing agents, resulting in greater transfection efficiency compared to ABP alone.

135 to be cell line specific, COS7 showed higher transfection efficiency compared to SH-SY5Y.

136 The high transfection efficiency, controlled dosage delivery and

137 pecific immunoliposome nanocomplex with high transfection efficiency could overcome these limitations

138 This motivated us to systematically evaluate transfection efficiency, cytotoxicity, and complex stabi

139 Here, we investigated the transfection efficiency, cytotoxicity, osteogenic potent

140 Transfection efficiency data for the corresponding CD:pD

141 tinoic acid treatment resulted in nearly 50% transfection efficiency-defined as the proportion of tra

142 Variations in transfection efficiency, delivery-induced cytotoxicity a

143 Transfection efficiency depended upon the form of the li

144 ent gene delivery approaches suffer from low transfection efficiency due to physiological barriers li

145 SM-102 LNPs showed exceptionally improved transfection efficiency due to their ability to form a c

146 emonstrate that MC can significantly improve transfection efficiency, duration of transgene expressio

147 neas with chloroquine and EDTA increased the transfection efficiency eight-fold and threefold, respec

148 arantee the effectiveness: 2 folds or higher transfection efficiency enhancement and rapid transgene

149 Cell viability and electro-transfection efficiency (eTE) are dependent on various e

150 n gene expression studies, especially in low transfection efficiency experiments; and (c) facilitatio

151 arations containing largely intact DNA; (ii) transfection efficiencies for the development of stable

152 ser energy and determined cell viability and transfection efficiency for both irradiation regimes.

153 tro transfection studies determined a higher transfection efficiency for each cyclic PEI sample when

154 Recently, unprecedented transfection efficiency for primary endothelial cells ha

155 trated to be the critical factor determining transfection efficiency for these polymers, mediating ef

156 tructural elements that generate the highest transfection efficiency for this new type of cationic li

157 ures as high as 9 atm leads to a increase in transfection efficiency from 1.7+/-.5 to 62+/-3.9% and a

158 Transfection efficiencies >95% were obtained by selectin

159 Maximum transfection efficiency (> 90%) requires a 2-hour incuba

160 High, stable transfection efficiencies in human ES cells have been di

161 Transfection efficiencies in isolated rat hepatocytes ap

162 It manifested high transfection efficiencies in muscle tissues, while signi

163 eted polyplexes showed up to 2.5 fold higher transfection efficiency in 4T1 murine mammary cancer cel

164 -based gene delivery systems were tested for transfection efficiency in a variety of cell lines, incl

165 f optimizing LNP composition to enhance mRNA transfection efficiency in adipocytes, providing a promi

166 nverted hexagonal CL-DNA complexes show high transfection efficiency in cell culture.

167 PE at a 1:1 molar ratio mediated the highest transfection efficiency in cell culture.

168 imately an order of magnitude improvement on transfection efficiency in CFBE41o- cells.

169 As with previous systems, strong transfection efficiency in comparison with commercial st

170 Transfection efficiency in cultured cells was dependent

171 achieve a good balance between toxicity and transfection efficiency in gene delivery systems.

172 n:PEI at a weight ratio of 5:5 showed higher transfection efficiency in HEK293, 3T3 and PC3 cells tha

173 e MessengerMAX followed by PBAE/LNP for mRNA transfection efficiency in HEK293T cells in vitro.

174 Transfection efficiency in HeLa and NIH/3T3 cells were c

175 n system that enables high levels of in vivo transfection efficiency in lung macrophages, yielding du

176 cantly improved cellular internalization and transfection efficiency in macrophages, depending on the

177 ationic liposomes resulted in an increase in transfection efficiency in mammalian cell lines.

178 ce protein (GFP), we achieved a sonoporation transfection efficiency in rate aortic smooth muscle cel

179 ery vectors) with alanine nearly doubles its transfection efficiency in the presence of serum and als

180 2-kDa PEI yields a nontoxic polycation whose transfection efficiency in the presence of serum is 400

181 the new multivalent lipid greatly increases transfection efficiency in the regime of small molar rat

182 The transfection efficiency in vitro increased significantly

183 of the aliphatic chain length (n = 12-18) on transfection efficiency in vitro was determined using ca

184 trate brain extracellular matrix to increase transfection efficiency in vivo.

185 ciated with substantial improvements in mRNA transfection efficiency included alkyl tail length of th

186 pNT in polyplexes prevented the reduction of transfection efficiency induced by a low temperature.

187 er, lack of efficient targeted delivery, low transfection efficiency, instability to nucleases, poor

188 intravenous (IV) injection achieved 73% mRNA transfection efficiency into lung endothelial cells, whi

189 stem cells (ADSCs) and astrocytes, and high transfection efficiency is achieved (77% in human ADSCs

190 Here, we tested the hypothesis that DNA transfection efficiency is limited by a simple physical

191 However, the transfection efficiency is poor and there is a lack of i

192 ratio) are important factors that determine transfection efficiency, lipid-DNA complex preparations

193 The predictable transfection efficiency, low toxicity, and ability to ly

194 that encode secreted proteins, however, low transfection efficiency may not preclude bio-activity of

195 ical limit; new methods designed to increase transfection efficiency must increase DNA concentration

196 mRNA/Ser-CART transfection efficiencies of >95% are achieved in vitro.

197 aematopoietic progenitor CD34(+) cells, with transfection efficiencies of 46.3 +/- 5.2%, 23.0 +/- 2.1

198 demonstrating 2 to 126-fold higher in vitro transfection efficiencies of different cell types in com

199 The transfection efficiencies of different groups of peptide

200 In this study, we evaluated transfection efficiencies of mRNA delivered in naked and

201 sts to CL-DNA complexes, where the optimized transfection efficiencies of multivalent and monovalent

202 t, while CaCO(3)-based nanoparticles improve transfection efficiencies of pDNA miR-200c, the ratio of

203 The transfection efficiencies of PSP condensates for deliver

204 possible strategies to significantly improve transfection efficiencies of synthetic gene vectors.

205 Transfection efficiencies of the modified BACs into huma

206 Despite the high transfection efficiencies of the viral vectors, their im

207 In vitro transfection efficiencies of these complexes were determ

208 E2F2 and EGFP in corneal endothelium with a transfection efficiency of 10% to 12%, using the pIRES2-

209 tment of HMEC-1 cells at N:P 6 resulted in a transfection efficiency of 33.7%, negligible cytotoxicit

210 Microporation also yielded a transfection efficiency of 93% and an average viability

211 In vivo, transfection efficiency of a plasmid co-expressing hSef-

212 ficient adenovirus mutant dl312 enhanced the transfection efficiency of a plasmid DNA-expressing beta

213 study has investigated the encapsulation and transfection efficiency of cationic liposomes prepared f

214 ) in combination with liposomes enhanced the transfection efficiency of cationic liposomes.

215 the past decade since they can maintain the transfection efficiency of commercially available, 25k b

216 an optimized transfection protocol in which transfection efficiency of Drosophila Schneider 2 cells

217 These peptides uniquely enhance the transfection efficiency of GFP-encoded plasmid DNA (pRmH

218 In contrast, the transfection efficiency of H(II)(C) complexes is indepen

219 d saturates for at a value rivaling the high transfection efficiency of H(II)(C) complexes.

220 Harnessing the high transfection efficiency of LNPs in antigen-presenting ce

221 stabilized, which significantly compromises transfection efficiency of materials shown to be effecti

222 Interestingly, the transfection efficiency of mDC2 with plasmid DNA vectors

223 acidification and the macrophage association/transfection efficiency of mRNAs.

224 an important prerequisite for improving the transfection efficiency of non-viral vector-mediated gen

225 o P. falciparum, we observed that the higher transfection efficiency of P. knowlesi resulted in near

226 e infection efficiency of adenovirus and the transfection efficiency of plasmid DNA in the presence o

227 -shifting moieties, impacts on the long-term transfection efficiency of polycationic vectors.

228 oly(L-lysine) and protamine, can enhance the transfection efficiency of several types of cationic lip

229 histocultures, and promotes the delivery and transfection efficiency of siRNA-lipoplexes under the lo

230 The transfection efficiency of SN carrying a reporter gene w

231 This, and the high heterogeneity and low transfection efficiency of the Caco-2 cell line prompted

232 traction of plasma membrane cholesterol, the transfection efficiency of the gene delivered by dendrim

233 In comparison to transfection efficiency of the homologous expression pla

234 iated enhanced cell uptake and high in vitro transfection efficiency of the polyplexes were demonstra

235 The transfection efficiency of the stable complex in 15% fet

236 The DNA condensation ability and gene transfection efficiency of these LMWP peptides were test

237 present important factors in determining the transfection efficiency of this hybrid expression system

238 To further improve the transfection efficiency of this targeting complex, a syn

239 ss colloidal-osmotic swelling, we achieved a transfection efficiency of ~20%, regardless of the prese

240 he proposition that the strong dependence of transfection efficiency on the oxidation state of BFDMA,

241 Vero cells demonstrated an approximately 20% transfection efficiency, only 0.5% of transfected cells

242 achieve, especially for cell lines with low transfection efficiency or when expression of multiple g

243 forming denpol showed significantly improved transfection efficiency over Lipofectamine in serum-cont

244 The presence of HoKC increased the in vitro transfection efficiency over that of the original comple

245 Transfection efficiency peaked at ~10% at 8h post sonica

246 he Flt23k plasmid was evaluated by measuring transfection efficiency (percentage of cells expressing

247 However, until now, low transfection efficiency, poor tissue penetration, and no

248 , this histidine-rich tail markedly improved transfection efficiency, presumably by increasing the bu

249 of transfecting human cells in culture with transfection efficiencies ranging from 0.3% to 4.1%, whi

250 t of our effort to investigate the structure-transfection efficiency relationships of self-assembled

251 ffect of pore behavior on cell viability and transfection efficiency remain poorly elucidated.

252 th protein types, cellular density/function, transfection efficiency, reporter dosage, secretion sign

253 ding potential injury to DRG neurons and low transfection efficiency, respectively.

254 The high transfection efficiency seen in the BHK cells allows stu

255 delivery, which overcome the problem of poor transfection efficiency seen with the plasmid-based syst

256 ion efficiencies and approximately 2% at low transfection efficiencies, simultaneous homozygous knock

257 gene delivery, albeit with unacceptably low transfection efficiencies (TE).

258 ed that BHH cell hybrids seem to have better transfection efficiencies than either of the parental ce

259 elded polyplexes afford up to 10-fold higher transfection efficiencies than the analogous stably shie

260 y, are stable at 5 C and demonstrated higher transfection efficiency than positive control experiment

261 containing DOPE showed substantially higher transfection efficiency than those formulated with DOPC,

262 ome formulations showed the highest in vitro transfection efficiency, the fluid and solid phase lipos

263 ruct, such as PAC DNA, substantially reduces transfection efficiency, the size effect of a DNA fragme

264 expression in COS-1 cells and correction for transfection efficiency, the Trp(173) allozyme displayed

265 pler structure, better stability, and higher transfection efficiency; therefore it may become a novel

266 th all of the cell lines used, regardless of transfection efficiency, time of processing posttransfec

267 overall transfection rate and widened their transfection efficiency to include not only RPE but phot

268 The triethylphosphonium polymer shows transfection efficiency up to 65% with 100% cell viabili

269 sphate (P) ratio of 10 and characterized for transfection efficiency using human bone marrow stromal

270 The transfection efficiencies vary as a function of the PEI/

271 The transfection efficiency was 1.2% based on green fluoresc

272 Mean transfection efficiency was 89.0% (SD 1.9).

273 The transfection efficiency was also found to be LpDNA dose-

274 ight, and %contraction band necrosis assays; transfection efficiency was assessed using fluorescent m

275 ection, suggesting that the NP-mediated mRNA transfection efficiency was consistent with the endocyto

276 Transfection efficiency was determined at 24 hours by nu

277 Transfection efficiency was determined with FITC-labeled

278 Transfection efficiency was determined with fluorescein-

279 Transfection efficiency was estimated as the percentage

280 Transfection efficiency was estimated by GFP expression.

281 Transfection efficiency was evaluated by green fluoresce

282 Transfection efficiency was further increased by additio

283 Transfection efficiency was maximized at rho = 0.4.

284 following a single administration, and their transfection efficiency was not attenuated by repeated a

285 High transfection efficiency was observed into both CD138(+)

286 Based on reporter plasmid expression, transfection efficiency was sixfold higher in ischemic m

287 for erythropoietin production and retroviral transfection efficiency, we chose to use HepG2 cells to

288 Their transfection efficiencies were at least two orders of ma

289 No discernible differences in transfection efficiencies were found between K-1735 clon

290 Transfection efficiencies were monitored by cotransfecti

291 reas fibroblasts, with up to 50-fold greater transfection efficiency, were less potent.

292 nt in pDNA loading, intracellular uptake and transfection efficiency, when compared to NPs lacking th

293 delivery strategy would facilitate improved transfection efficiency while eliminating the toxicity s

294 Sucrose didn't affect the transfection efficiency, while sorbitol resulted in a fl

295 es with nucleic acids and provided very high transfection efficiency with all nucleic acids and cell

296 In contrast, transfection efficiency with DNA reaches its maximum at

297 ionic lipid formulations to have a high mRNA transfection efficiency with low cytotoxicity.

298 a significant interest because of their high transfection efficiency with low toxicity.

299 ary human T cells and demonstrated up to 95% transfection efficiency with minimum impact on cell viab

300 MCMs), we observed up to 4-fold increases in transfection efficiency with simultaneous reductions in