ライフサイエンスコーパス: transit peptide

1 r 15 amino acids was included carboxy to the transit peptide.

2 larger precursor, preToc75, with a bipartite transit peptide.

3 , recognizing the C-terminal residues of the transit peptide.

4 side in the C-terminal 10-15 residues of the transit peptide.

5 sisting of the C-terminal 12 residues of the transit peptide.

6 t precursor proteins, removing an N-terminal transit peptide.

7 small Cys cluster proteins with a cleavable transit peptide.

8 s in that its precursor form has a bipartite transit peptide.

9 This gene lacks a predicted chloroplast transit peptide.

10 , N-terminal targeting sequence known as the transit peptide.

11 d to the chloroplast, removing an N-terminal transit peptide.

12 ophobic region in the C-terminal half of the transit peptide.

13 an apparent N-terminal chloroplast-targeting transit peptide.

14 his PHGPX encodes a recognisable chloroplast transit peptide.

15 association of bacterial chaperones with the transit peptide.

16 acid polypeptide that included a 60-residue transit peptide.

17 oplasts by the addition of an amino-terminal transit peptide.

18 her processed/modified beyond removal of the transit peptide.

19 ng frame that included a putative plastidial transit peptide.

20 ritical role of its NH2-terminal chloroplast transit peptide.

21 the estimated size (approximately 6 kD) of a transit peptide.

22 nteraction is mediated primarily through the transit peptide.

23 ynthase may represent an organelle-targeting transit peptide.

24 pped to the C-terminal 20 amino acids of the transit peptide.

25 g predicted to contain a cleavable signal or transit peptide.

26 sing but retains most of the electropositive transit peptide.

27 the cDNA was predicted to have a chloroplast transit peptide.

28 e type-III effectors represent a chloroplast transit peptide.

29 hodanese domain, and a predicted chloroplast transit peptide.

30 tCML30 utilizes an N-terminal, non-cleavable transit peptide.

31 ow that AtD27 possesses a functional plastid transit peptide.

32 targeted to the chloroplast using the rbcS1 transit peptide.

33 est that cpTatC possesses a stroma-targeting transit peptide.

34 y identical, predicted 48-amino acid plastid transit peptide.

35 aining protein (SBDCP1) possessing a plastid transit peptide.

36 t2g32040 protein has a predicted chloroplast transit peptide.

37 were expressed as fusions with a plastidial transit peptide.

38 rmini, which share features with chloroplast transit peptides.

39 ecursor proteins with functional chloroplast transit peptides.

40 t absence of Arabidopsis photolyases bearing transit peptides.

41 extensions that resemble typical chloroplast transit peptides.

42 st by stroma-targeting domains in N-terminal transit peptides.

43 ite located at the C-terminus of chloroplast transit peptides.

44 sequence had features similar to chloroplast transit peptides.

45 eukaryotic species had predicted chloroplast transit peptides.

46 n the thylakoid with unprocessed chloroplast transit peptides.

47 sor (preprotein) form, each with a cleavable transit peptide.(4)(,)(5)(,)(6)(,)(7)(,)(8) Preproteins

48 y indicate that beta-barrels that do not use transit peptides also enter the chloroplast using compon

49 Transit peptide analysis predicted two cytosolic and fou

50 sing MALDI-TOF, as well as transmembrane and transit peptide analysis.

51 dicted this affinity pattern for >75% of the transit peptides analyzed in the chloroplast transit pep

52 ced beet CMO amino acid sequence comprised a transit peptide and a 381-residue mature peptide that wa

53 SPP contains a specific binding site for the transit peptide and additional proteolysis by SPP trigge

54 Upon cleavage of the transit peptide and additional proteolytic processing, m

55 proximately 25 kDa, containing a chloroplast transit peptide and an acidic alpha-helical region.

56 ptides corresponding to other regions of the transit peptide and control peptides promoted significan

57 Fusions containing the entire transit peptide and either of the first two introns yiel

58 that are predicted not to have a chloroplast transit peptide and expressed them in the yeast Saccharo

59 transferases; it has a predicted chloroplast transit peptide and is plastid localized.

60 FtsHi1 bears a predicted chloroplast transit peptide and localizes to the chloroplast envelop

61 argeting functions of the two domains of the transit peptide and of the mature region of prOEP75, we

62 PSB33 encodes a protein with a chloroplast transit peptide and one transmembrane segment.

63 hloroplast extract rapidly degraded both the transit peptide and subfragment.

64 Interaction between the transit peptide and the bilayer was very rapid and could

65 novel tool to dissect interactions between a transit peptide and the chloroplast translocation appara

66 of a fusion that includes the entire plastid transit peptide and the first two introns of PAT1 had on

67 A chloroplast transit peptide and the napin promoter were fused to the

68 sing peptidase depended on the nature of the transit peptide and the passenger protein, and increased

69 (GOR2) does not encode a pre-protein with a transit peptide and therefore is most likely to represen

70 inal domain of the TGD2 sequence lacking the transit peptide and transmembrane sequences was fused to

71 proteins and to degrade cleaved chloroplast transit peptides and damaged, misfolded, or otherwise un

72 ize performance and accuracy for chloroplast transit peptides and demonstrate this technique on the p

73 er but have predicted N-terminal chloroplast transit peptides and lack transmembrane domains, consist

74 ma-like proteins have functional chloroplast transit peptides and thus are likely candidates for chlo

75 29 kD (mature Ee-BAM1 after cleavage of the transit peptide) and a 35 kD (unprocessed EeBAM1) protei

76 His-tag, but lacking most of the N-terminal transit peptide, and after purification was found to hav

77 e chloroplast, that its first exon acts as a transit peptide, and that the smaller protein is cytosol

78 olecular mass preproteins with an N-terminal transit peptide, and then posttranslationally imported f

79 psis AtRBSK contains a predicted chloroplast transit peptide, and we confirmed plastid localization u

80 presequence had features similar to plastid transit peptides, and processing of the LAP-N presequenc

81 the bipartite nature of the Chlamydomonas PC transit peptide appears similar to that of lumen-targete

82 g depends on the same region, although their transit peptides are highly divergent in primary sequenc

83 Chloroplast transit peptides are necessary and sufficient for the ta

84 Free transit peptides are thought to be toxic to the plastid

85 g green fluorescent protein (GFP) fused to a transit peptide as a reporter, we examined import into c

86 e, with and without the putative chloroplast transit peptide, as well as five chimeric cytosolic/plas

87 ther eukaryotic organisms and has a putative transit peptide at its amino terminus.

88 Hence, transit peptide binding and removal are two separable st

89 e steps of precursor processing by SPP (i.e. transit peptide binding, removal, and conversion to a de

90 envelope protein 80 kD (OEP80), also uses a transit peptide but has a distinct envelope sorting sign

91 both introns, but constructs containing the transit peptide but no introns give rise to much lower l

92 The cpx1 gene encodes the expected plastid transit peptide, but this region is deleted from the cpx

93 suppress immunity required their respective transit peptides, but the AvrRps4-induced HR did not.

94 ing, SPP terminates its interaction with the transit peptide by a second cleavage, converting it to a

95 perimentally the presence of a chloroplastic transit peptide by showing that the product of the nucle

96 pose that, after cleavage of the chloroplast transit peptide by stromal processing peptidase, additio

97 transit peptides analyzed in the chloroplast transit peptide (CHLPEP) database.

98 Based on the probable transit peptide cleavage site the mature protein is 45.7

99 presence of a small intron near the putative transit peptide cleavage site.

100 Post-translational transit peptide cleavage sites for the maturation of the

101 Sites for transit peptide cleavages in the cytosolic RP precursors

102 in vivo, deletions were introduced into the transit peptide coding region of the petE gene, which en

103 nin transit peptide was replaced by the AtpC transit peptide-coding region allowed plastocyanin to ac

104 stroma targeting, mutant and wild-type AtpC transit peptide-coding regions were fused to the bacteri

105 l four Arabidopsis proteins have a predicted transit peptide consistent with targeting to the inner e

106 e precursor binding and cleavage followed by transit peptide conversion to a degradable substrate.

107 Transit peptide conversion to a subfragment also depends

108 orophytes evolved by integrating chloroplast transit peptide (cTP), and N-terminal domains to the ATP

109 res sequences in addition to the chloroplast transit peptide (cTP).

110 Various chloroplast transit peptides (CTP) have been used to successfully ta

111 Central to our strategy is the chloroplast-transit-peptide (CTP), crucial for optimal oxygenation.

112 cal and structural properties of chloroplast transit peptides (cTPs).

113 We hypothesized that FLN may participate in transit peptide degradation in the apicoplast based on i

114 in (GFP)-fused mislocalized PGK mutants, the transit peptide deletion mutant (NO TRANSIT PEPTIDE [NOT

115 associated with chloroplasts, proteins with transit peptide deletions remained almost entirely cytos

116 To determine whether the transit peptide deletions were impaired in in vivo strom

117 and a cytoplasmic CP mutant lacking the dual transit peptide (DeltaNtCP).

118 thermore, elongation factor1a fused with the transit peptide derived from chl-PGK or with a Rubisco s

119 on, we have developed a novel epitope-tagged transit peptide derived from the precursor of the small

120 The CF1-gamma transit peptide does have an in vivo stroma-targeting fu

121 al sequence followed by a positively charged transit peptide domain.

122 A putative amino-terminal transit peptide encoded by the GS2 cDNA suggests that th

123 The ORF excluding transit peptides encoded a 64.9 kDa protein that was exp

124 of ho1 was fused in frame with a chloroplast transit peptide-encoding sequence from the oli gene of A

125 r GPT1 in plastids, whereas GPT1 without the transit peptide (enforcing ER/peroxisomal localization)

126 However, the mechanism by which transit peptides engage the translocation apparatus has

127 econd, the fate of GFP fused to a ferredoxin transit peptide (FD5-GFP) was determined.

128 Both clones also contain putative transit peptides followed by the VRAA(E)A motif, the con

129 ith an amino terminus that may function as a transit peptide for localization in plastids.

130 monas aeruginosa (PaAPR) fused with the rbcS transit peptide for localization of the protein to plast

131 uence at the amino terminus that resembles a transit peptide for localization to mitochondria or plas

132 uence at the amino terminus that resembles a transit peptide for localization to plastids.

133 idenced by the presence of an amino-terminal transit peptide for plastid localization in APR1 and APR

134 edicts a previously unrecognized chloroplast transit peptide for the ToxA effector, which we show tra

135 The apparent affinity of chloroplast transit peptides for chloroplast lipids and the tendency

136 embers encode proteins that possess apparent transit peptides for chloroplast stromal localization.

137 Recombinant SPP removed the transit peptide from a variety of precursors in a single

138 ding to 855 bp of 5' promoter region and the transit peptide from lambdaGK.1,a genomic clone encoding

139 ed in the C-terminal portion of the preToc75 transit peptide from six plant species.

140 In vitro cleavage by FLN of the transit peptide from the apicoplast-resident acyl carrie

141 in vitro import, whereas replacement with a transit peptide from the gamma-subunit of chloroplast AT

142 and its replacement with a bona fide plastid transit peptide from the glutamine synthetase 2 gene doe

143 cessing peptidase (SPP) catalyzes removal of transit peptides from a diversity of precursor proteins

144 these proteins are divergent, in contrast to transit peptides from other proteins targeted to the thy

145 To examine Chlamydomonas transit peptide function in vivo, deletions were introdu

146 lerated nucleotide exchange, indicating that transit peptides function as GTPase-activating proteins

147 s using a chimeric pre-protein (plastocyanin transit peptide fused to dihydrofolate reductase; PC-DHF

148 ure small subunit, glutathione S-transferase-transit peptide fusion protein, and SS-tp in dye release

149 C1 and TPS26 are predicted to encode plastid transit peptides; fusion proteins of green fluorescent p

150 Cleavage of its predicted transit peptide gives a mature protein of Mr 20k.

151 the early stage and a later stage after the transit peptide has been removed, suggesting that cpHsc7

152 differences, the Chlamydomonas plastocyanin transit peptide has functional domains similar to those

153 lace the passenger protein C-terminal to the transit peptide, His-S-SStp bound to the translocation a

154 The recombinant transit peptide, His-S-SStp, contains a removable dual-e

155 analysis of infA sequences and assessment of transit peptide homology indicate that the four nuclear

156 ysis by psToc34 is stimulated by chloroplast transit peptides; however, this activity is not stimulat

157 ng the stromal targeting domain of the Toc75 transit peptide in Escherichia coli, using the intein-me

158 te and that loss of a functional chloroplast transit peptide in N. munroi CA1a is associated with the

159 gene fused to the sequence of a chloroplast transit peptide in the nuclear genome.

160 Transit peptides in Chlamydomonas reinhardtii are consid

161 2 builds experimentally annotated signal and transit peptides in orientations that point away from th

162 efficiency is phosphorylation of chloroplast transit peptides in the cytosol.

163 entry as another common property of membrane-transiting peptides in addition to their ability to cros

164 Although MKS1 does not contain a classical transit peptide, in vitro import assays showed that it w

165 Removal of the transit peptide increased the apparent overall activity

166 on of the transit peptide suggested that the transit peptide induced a dramatic reorganization of lip

167 E1 and CD, along with their proximity to the transit peptide, influence translocation.

168 epresent the first direct visualization of a transit peptide interacting with the chloroplast translo

169 as determined and used to divide the prOEP75 transit peptide into N- and C-terminal domains.

170 Although the transit peptide is both necessary and sufficient to dire

171 Once in the stroma, the POR transit peptide is cleaved off and the mature POR protei

172 A plastid transit peptide is located at the N terminus of OsGR3, a

173 Thus, phosphorylation of the transit peptide is not responsible for the specificity o

174 A putative transit peptide is present at the N terminus.

175 The N-terminal portion of the Toc75 transit peptide is sufficient to target the protein to t

176 CD2 protein contains a predicted chloroplast transit peptide, is processed in vivo, and purifies with

177 which lacks the putative plastid-localizing transit peptide, is unable to rescue ssi2-triggered phen

178 ecursor of SPP, containing an unusually long transit peptide itself, is not proteolytically active.

179 t-targeted protein while the other encodes a transit peptide-less protein that accumulates in the cyt

180 Since the transit peptide-mature protein cleavage site could not b

181 asts and root leucoplasts and identified two transit-peptide motifs that specifically enhance preprot

182 rates by selective evolutionary retention of transit-peptide motifs, which enhances import into speci

183 nts, the transit peptide deletion mutant (NO TRANSIT PEPTIDE [NOTP]-PGK-GFP) and the nucleus location

184 222 amino acid residues including a putative transit peptide of 28 amino acids.

185 ptide of 622 amino acid residues including a transit peptide of 39 amino acids.

186 has a molecular mass of 101 kDa including a transit peptide of 48 amino acids.

187 ellow fluorescent protein (YFP) fused to the transit peptide of EPSP synthase* or the small subunit o

188 E1 or its catalytic domain, CD, fused to the transit peptide of ferredoxin or ribulose-bisphosphate c

189 ments using synthesized oligopeptides of the transit peptide of ferredoxin precursor to investigate t

190 we also demonstrated that processing of the transit peptide of nuclear-encoded apicoplast proteins r

191 ort by creating a series of deletions in the transit peptide of plastocyanin and determining their ef

192 is maintained by specific recognition of the transit peptide of preproteins by the coordinate activit

193 stic protein import assays revealed that the transit peptide of prOEP75 is bipartite in that the N- a

194 Phosphorylation of the transit peptide of several chloroplast-targeted proteins

195 nas reinhardtii by creating deletions in the transit peptide of the gamma-subunit of chloroplast ATPa

196 The transit peptide of the precursor of ferredoxin is releas

197 The interaction between SStp, the transit peptide of the precursor protein to the small su

198 r to remove the phosphorylation site) of the transit peptide of the small subunit of ribulose bisphos

199 Surprisingly, fusing the transit peptide of the small subunit of Rubisco with mat

200 By fusing the putative transit peptides of ClpB3 and ClpB4 with GFP, we showed

201 Similar alterations to the transit peptides of histidyl- or cysteinyl-tRNA syntheta

202 the mature proteins are well conserved, the transit peptides of these proteins are divergent, in con

203 Toc159 bind directly and selectively to the transit peptides of these representative photosynthetic

204 on, we show that Hsp93 directly binds to the transit peptides of various preproteins undergoing activ

205 The ORF, excluding the transit peptide, of this cDNA was expressed in E. coli,

206 Toc75 is directed with a cleavable bipartite transit peptide partly via the general import pathway, w

207 In vitro transit peptide processing and chimeric precursor import

208 A encodes a type-I IPPI containing a plastid transit peptide (PTP) at its amino terminus.

209 chloroplasts by the commonly-used rice rbcS transit peptide (rCTP) and were subsequently degraded.

210 3 amino acid residues from the center of the transit peptide reduced in vitro import to an undetectab

211 mature protein was immediately released, the transit peptide remained bound to SPP.

212 ranslation product, the mature protein after transit peptide removal, and the coding sequence of the

213 irectly imported into chloroplasts through a transit peptide residing in the N-terminal 50 amino acid

214 s first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 a

215 letions within the C-terminal portion of the transit peptide resulted in the appearance of import int

216 the full-length protein (minus the putative transit peptide) resulted in induction of 24.5 kDa (majo

217 Cleavage of the transit peptide results in a mature putative polypeptide

218 monas by fusing them to a Chlamydomonas rbcS transit peptide sequence engineered to contain rbcS intr

219 The ASA2 cDNA contains a putative transit peptide sequence, and Southern hybridization sho

220 Removal of the cleavable, N-terminal transit peptide sequences greatly affected isoelectric p

221 The transit peptide sequences of the nuclear infA genes from

222 nucleus, complete with putative chloroplast transit peptide sequences.

223 drophobic residues similar in composition to transit peptide sequences.

224 The interaction was specific for the transit peptide since His-S alone did not engage the chl

225 lipid bilayers (liposomes) with the purified transit peptide (SS-tp) of the precursor form of the sma

226 ursor (prSSU), the mature domain (mSSU), the transit peptide (SS-tp), and three C-terminal deletion m

227 consistent set of features common to the DXS transit peptides studied.

228 dase activity responsible for degradation of transit peptide subfragments suggests that it may recogn

229 e liposomes before and after addition of the transit peptide suggested that the transit peptide induc

230 ffinity for the N terminus of SStp and other transit peptides supports a molecular motor model in whi

231 were shown by in vitro uptake to function as transit peptides, targeting these proteins into the chlo

232 ion construct of HCF222 containing a plastid transit peptide targets the protein into chloroplasts an

233 tion that each of four structurally distinct transiting peptides tested displayed antiviral activity

234 pliced mLKB1 variant encodes a mitochondrial transit peptide that allows it to localize to the mitoch

235 r, AZI1 does not possess a classical plastid transit peptide that can explain its localization.

236 mport pathway is mediated by features of the transit peptide that determine precursor binding and cle

237 nucleus and is synthesized with a bipartite transit peptide that is cleaved during maturation.

238 an extra amino-terminal domain following the transit peptide that is highly conserved from cyanobacte

239 xperiments indicated that AtMinD1 contains a transit peptide that targets it to the chloroplast.

240 ehensive computer analyses revealed putative transit peptides that are predicted to target the enzyme

241 are synthesized as precursors with bipartite transit peptides that contain information for uptake and

242 Many proteins contain cleavable signal or transit peptides that direct them to their final subcell

243 ene evolution: the origin of presequences or transit peptides that generally exist in nucleus-encoded

244 psis, like FtsZ1 proteins, contain cleavable transit peptides that target them across the outer envel

245 After removal of the predicted transit peptide, the mature 480-residue GTR has a calcul

246 l retargeting of the enzyme by addition of a transit peptide to a cytoplasmic Delta9 desaturase rathe

247 SPP converted the transit peptide to a subfragment form that it no longer

248 distinct type-III effectors use a cleavable transit peptide to localize to chloroplasts, and that ta

249 ile most chloroplast proteins use N-terminal transit peptides to enter the chloroplasts through the t

250 tances, chloroplast targeting information (a transit peptide (TP) from a pea rbcS gene) was incorpora

251 sion constructs containing only the putative transit peptide (TP) of LEM1 localize exclusively to the

252 Despite the availability of thousands of transit peptide (TP) primary sequences, the structural a

253 nding proteins with and without a N-terminal transit peptide (TP), respectively.

254 taining an N-terminal extension known as the transit peptide (TP).

255 usly, we identified the N-terminal domain of transit peptides (TPs) as a major determinant for the tr

256 previously unrecognized ATP-requirement for transit peptide turnover.

257 isoform 1 in transgenic soybean without its transit peptide under the control of the 35S CaMV promot

258 ana scions expressing GFP-tagged chloroplast transit peptides under the 35S promoter onto non-transge

259 Quantitation of the bound transit peptide was determined by flow cytometry, showin

260 stable interaction between SPP and an intact transit peptide was directly demonstrated using a newly

261 The cDNA excluding its transit peptide was expressed in E. coli, and the corres

262 esidue enzyme without a putative chloroplast transit peptide was expressed in Escherichia coli and pu

263 entially at the dimer interface, whereas the transit peptide was found at both regions equally.

264 0-mers), the lipid-interacting domain of the transit peptide was partially mapped to the C-terminal 2

265 stroma-targeting domain of the plastocyanin transit peptide was replaced by the AtpC transit peptide

266 (a) The small subunit transit peptide was sufficient to provide import of this

267 , excluding the sequence for the chloroplast transit peptide, was codon optimized and expressed in Es

268 fACP containing an amino-terminal apicoplast transit peptide, was not a substrate for pfMCAT.

269 LeAOS, which contains a typical N-terminal transit peptide, was targeted to the inner envelope memb

270 nown function but with predicted chloroplast transit peptides were identified, of which 17 (63%) are

271 es the ability to proteolytically remove the transit peptide when residues of the HXXEH motif, found

272 truncated version of the protein lacking its transit peptide, which allowed targeting to the plasma m

273 H-1 protein possesses a predicted N-terminal transit peptide, which directs green fluorescent protein

274 ABCs, however, atABC1 contains an N-terminal transit peptide, which targets it to chloroplasts.

275 the sorting of AtTic40 requires a bipartite transit peptide, which was first cleaved by the stromal

276 VDE is nuclear encoded and has a transit peptide with characteristic features of other lu

277 SPP releases a subfragment consisting of the transit peptide without its original C terminus.