ライフサイエンスコーパス: transposase

1 ntigs containing KPC-2 within an ISKpn6-like transposase.

2 ical and have different affinities for their transposase.

3 (tnsABCDE) including an atypical heteromeric transposase.

4 e construct flanked by binding sites for the transposase.

5 lex Virus thymidine kinase (HSV-tk) with the transposase.

6 s1 evolved from casposases, a novel class of transposases.

7 ntally distinct from other cut-and-paste DNA transposases.

8 icted proteins homologous to RCR prokaryotic transposases.

9 chanism similar to retroviral integrases and transposases.

10 representing another approach to manipulate transposases.

11 those generated by retroviral integrases and transposases.

12 ed gene loss limited to regions encoding for transposases.

13 uyveromyces lactis hobo/Activator/Tam3 (hAT) transposase 1 (Kat1), operating at the fossil imprints o

14 X-ray structures of ISCth4 transposase, a member of the IS256 family of insertion s

15 oprecipitation, DNase I hypersensitivity and transposase-accessibility assays combined with high-thro

16 n be tracked by single-cell RNA or assay for transposase accessible chromatin (ATAC) sequencing.

17 Using the assay of transposase accessible chromatin (ATAC-seq), we observe

18 single-cell combinatorial indexing assay for transposase accessible chromatin (sci-ATAC-seq); a softw

19 ychiatric disorders we applied the Assay for Transposase Accessible Chromatin followed by sequencing

20 Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) i

21 We utilized assay for transposase accessible chromatin sequencing (ATAC-seq) i

22 For example, assay for transposase accessible chromatin sequencing (ATAC-seq) i

23 DNaseI sequencing (DNase-seq) and Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) p

24 itation sequencing (ChIP-seq), and assay for transposase accessible chromatin using sequencing (ATAC-

25 Recent innovations in single-cell Assay for Transposase Accessible Chromatin using sequencing (scATA

26 re, the framework for applying the Assay for Transposase Accessible Sequencing (ATAC-seq) to biobanke

27 The assay for transposase-accessible chromatin (ATAC)-seq, coupled wit

28 We employed the assay for transposase-accessible chromatin (ATAC-seq) in four plan

29 The use of Assay for Transposase-Accessible Chromatin (ATAC-seq) to profile c

30 er assays (MPRAs) coupled with the assay for transposase-accessible chromatin (ATAC-Seq).

31 Single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) is the sta

32 se (MNase) digestion and ATAC-seq (assay for transposase-accessible chromatin [ATAC] using sequencing

33 bility, as determined by ATAC-seq (assay for transposase-accessible chromatin [ATAC] with high-throug

34 equencing (ChIP-seq) combined with assay for transposase-accessible chromatin coupled to high-through

35 Using the Assay for Transposase-Accessible Chromatin coupled to high-through

36 enhancer identification, using the assay for transposase-accessible chromatin followed by sequencing

37 d despite the discovery that the Analysis of Transposase-Accessible Chromatin followed by sequencing

38 Single-cell assay of transposase-accessible chromatin followed by sequencing

39 ble chromatin regions by using the Assay for Transposase-Accessible Chromatin from purified mouse car

40 Assaying transposase-accessible chromatin in single cell sequenci

41 Mapping transposase-accessible chromatin in single cells by sequ

42 ly parallel droplet-based method for mapping transposase-accessible chromatin in single cells using s

43 PC-enriched assay for transposase-accessible chromatin peaks, representing can

44 nd acetylated histone 3 lysine 27 as well as transposase-accessible chromatin profiling to dissect ci

45 Remarkably, genome-wide assay for transposase-accessible chromatin sequencing (ATAC-seq) a

46 atin accessibility profiling using assay for transposase-accessible chromatin sequencing (ATAC-seq) i

47 H) and T(FR) cells was assessed by assay for transposase-accessible chromatin sequencing (ATAC-seq) t

48 Transposase-accessible chromatin sequencing (ATAC-seq) w

49 Assay for transposase-accessible chromatin sequencing (ATAC-seq),

50 encing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq)

51 In this study, the assay for transposase-accessible chromatin sequencing was employed

52 knockdown approaches, CRISPR-Cas9, assay for transposase-accessible chromatin sequencing, and chromat

53 ngle-cell and bulk RNA sequencing, assay for transposase-accessible chromatin sequencing, and functio

54 ell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing.

55 and single-cell RNA sequencing and assay for transposase-accessible chromatin sequencing.

56 lowed by sequencing (ChIP-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-

57 Here we collected assay for transposase-accessible chromatin using sequencing (ATAC-

58 onous neuronal activation using an assay for transposase-accessible chromatin using sequencing (ATAC-

59 n of the chromatin landscape (as assessed by transposase-accessible chromatin using sequencing (ATAC-

60 In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-

61 ible genome of individual cells by assay for transposase-accessible chromatin using sequencing (ATAC-

62 (CNV), whole-exome sequencing, and Assay for Transposase-Accessible Chromatin using sequencing (ATAC-

63 Here we performed RNA sequencing, assay for transposase-accessible chromatin using sequencing (ATAC-

64 Here, we combined Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-

65 for histone modifications and 132 assay for transposase-accessible chromatin using sequencing (ATAC-

66 Assay for Transposase-Accessible Chromatin using sequencing (ATAC-

67 g was accomplished by employing an Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-

68 eq), RNA sequencing (RNA-seq), and assay for transposase-accessible chromatin using sequencing (ATAC-

69 Assay for transposase-accessible chromatin using sequencing (ATAC-

70 ous CD4+ T cells were analyzed by assays for transposase-accessible chromatin using sequencing (ATAC-

71 munoprecipitation with sequencing, assay for transposase-accessible chromatin using sequencing and Hi

72 Single-cell assay for transposase-accessible chromatin using sequencing confir

73 We performed Assay for Transposase-Accessible Chromatin using sequencing on hum

74 Assay for transposase-accessible chromatin using sequencing reveal

75 proach, named 'droplet single-cell assay for transposase-accessible chromatin using sequencing' (dscA

76 res and applied it to an ATAC-seq (assay for transposase-accessible chromatin using sequencing) data

77 -cell RNA sequencing and ATAC-seq (assay for transposase-accessible chromatin using sequencing) exper

78 seq]) and chromatin accessibility (assay for transposase-accessible chromatin using sequencing) induc

79 cipitation followed by sequencing, assay for transposase-accessible chromatin using sequencing, and i

80 cyte nuclei were assessed by using assay for transposase-accessible chromatin using sequencing.

81 -seq) profiling combined with bulk assay for transposase-accessible chromatin with high-throughput se

82 ibitory cortical neurons using the Assay for Transposase-Accessible Chromatin with high-throughput se

83 Using an assay for transposase-accessible chromatin with high-throughput se

84 We developed an allele-specific assay for transposase-accessible chromatin with high-throughput se

85 We used Assay for Transposase-Accessible Chromatin with high-throughput se

86 Using the assay for transposase-accessible chromatin with high-throughput se

87 ATAC-Seq (assay for transposase-accessible chromatin with high-throughput se

88 tomes using promoter capture Hi-C, assay for transposase-accessible chromatin with high-throughput se

89 Super-resolution imaging using assay for transposase-accessible chromatin with photoactivated loc

90 ll-specific Ribotag RNA profiling, assay for transposase-accessible chromatin with sequencing (ATAC-s

91 Further, results of assays for transposase-accessible chromatin with sequencing (ATAC-s

92 de RNAs and open chromatin sites by assay of transposase-accessible chromatin with sequencing (ATAC-s

93 et-based mitochondrial single-cell assay for transposase-accessible chromatin with sequencing (scATAC

94 global scan for open chromatin (assaying for transposase-accessible chromatin with sequencing).

95 Here we report assay of transposase-accessible chromatin with visualization (ATA

96 (3D ATAC-PALM) that integrates an assay for transposase-accessible chromatin with visualization, PAL

97 tu, we developed three-dimensional assay for transposase-accessible chromatin-photoactivated localiza

98 s H3K9ac, H3K27ac and H3K27me3; an assay for transposase-accessible chromatin; and RNA sequencing wer

99 he route of an intact DNA strand through the transposase active site before second strand cleavage.

100 A new study shows that aberrant DNA transposase activity promotes structural alterations tha

101 ts into its targeting and regulation of IstA transposase activity.

102 Expression of the transposase alone revealed no mobilization of endogenous

103 roscopy structures of the amphioxus ProtoRAG transposase (an evolutionary relative of RAG), we identi

104 ted early during the interaction between the transposase and a potential target site, which may be ho

105 oprotein intermediates demonstrated that the transposase and Cas9 moieties can bind their respective

106 The differences between transposase and CRISPR-Cas integrase are largely archite

107 irst structural study of a copy-out/paste-in transposase and demonstrate its ability to catalyze all

108 Tn5 transposase and DNase I sequencing-based methods prefer

109 action pathway of a eukaryotic cut-and-paste transposase and illuminate some of the earliest steps in

110 t in incomplete prophages, while tail fiber, transposase and integrase genes are significantly enrich

111 study, we present a method that combines Tn5 transposase and molecular identifiers for the highly acc

112 ow that two ancestral RAG1 proteins, Transib transposase and purple sea urchin RAG1-like, have a late

113 ly degenerate and appeared to have a mutated transposase and terminal sequences, while a second 3131

114 a single plasmid construct that combines the transposase and the transpositioning transgene element t

115 ansposition, interacting with both the TnsAB transposase and TnsD-attTn7.

116 to decrease post-transposition expression of transposase and to eliminate the cells that have residua

117 The use of PB in a plasmid containing both transposase and transposon greatly increased the probabi

118 ontal gene transfer between bacteria such as transposases and integrases) syntenic with ARGs were rar

119 rves as a model system for understanding DDE transposases and integrases.

120 CRISPR system, Eukaryotic-like proteins, and transposases), and in functions important for nutrient c

121 A and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that

122 e mobile DNAs, imprecision of integrases and transposases, and differential activity among identical

123 were capable of detecting 82% of the ISs and transposases annotated in GenBank with 80% sequence iden

124 stems, no helper plasmids are required since transposases are not expressed inside the host cells, th

125 Using the Hermes transposase as a guide, we constructed a 36-kDa "mini" R

126 DNase I and Tn5 transposase assays require thousands to millions of fres

127 through human CD46, a relatively safe SB100X transposase-based integration machinery, a micro-LCR-dri

128 ormation, and modifying ends with additional transposase binding sites stimulates activity.

129 s ordered assembly process shepherds primary transposase binding to the inner 12DRs (where cleavage d

130 Here we show that purified transposase binds specifically to the Muta1 ends and cat

131 h P-elements, HISR-N lines lost functional P-transposase but retained Har-P's that when crossed back

132 In vivo integrations by purified transposase can be achieved by electroporation, chemical

133 However, prolonged expression of transposase can become a potential source of genotoxic e

134 targeting, such as those catalyzed by active transposases, can generate DNA fragments that are used b

135 the in vitro activities of the putative Cas1 transposase ('casposase') from Aciduliprofundum boonei.

136 n-based systems that integrate transgenes by transposase-catalyzed "cut-and-paste" mechanism have eme

137 P element transposase catalyzes the mobility of P element DNA tran

138 peats (TIRs) contain sequences essential for transposase cleavage and have been implicated in DNA rep

139 critical for repair of DNA breaks following transposase cleavage in vivo.

140 In contrast, cut-and-paste transposases cleave two DNA strands of opposite polarity

141 Intrabiliary instillation of the transposon-transposase complex was coupled with lobar bile duct lig

142 deciphered several inverted terminal repeat-transposase complexes that are intermediates during tran

143 we varied nuclear preparation conditions and transposase concentrations and applied rigorous quality

144 and found that transposition declined after transposase concentrations became high enough for visibl

145 s (Fic; D-alanyl-D-alanine-carboxypeptidase; transposase) dated the divergence event at 300 million y

146 s establish engineered LPs as a new tool for transposase delivery.

147 We conclude that Kat1 is a highly regulated transposase-derived endonuclease vital for sexual differ

148 IP-seq or DNase-seq, without considering the transposase digested DNA fragments that contain addition

149 acid motif, which forms part of the mariner transposase dimer interface.

150 rucially, we find that each active site of a transposase dimer is responsible for two hydrolysis and

151 r basis for the reduced affinity of the Mos1 transposase DNA-binding domain for the left IR as compar

152 The crystal structure of the Hermes transposase-DNA complex reveals that Hermes forms an oct

153 chemical transfection or Lipofection of the transposase:DNA mixture, in contrast to other published

154 Mos1 transposase does not support target commitment, which ha

155 red specific catalytic residues in the PGBD5 transposase domain as well as end-joining DNA repair and

156 Although the Metnase transposase domain has been largely conserved, its catal

157 of Asn-610 with either Asp or Glu within the transposase domain significantly reduces ssDNA binding a

158 uires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containin

159 ) arose from a chimeric fusion of the Hsmar1 transposase downstream of a protein methylase in anthrop

160 agenic T2Onc2 transposon via expression of a transposase driven by the keratin K5 promoter in a p53(+

161 s appear to have originated from prokaryotic transposases (e.g. TN7 and Mu) and combine a CDC6/ORC1-S

162 Activation of the transposase efficiently generates fragment libraries wit

163 Consequently, the separation of the transposase element from the polyA sequence after transp

164 As a group, heteromeric transposase elements utilize diverse target site selecti

165 The Tn5 transposase enables the use of an index multiplexing str

166 n vivo, leading to the production of the non-transposase-encoding mature mRNA isoform in Drosophila g

167 anied by alteration of their function from a transposase/endonuclease to a heterochromatin protein, d

168 arly, fusion of a fluorescent protein to the transposase enhanced the transposition activity, represe

169 9 protein fused to the amino-terminus of the transposase enzyme designed to target the hypoxanthine p

170 We have developed a single-tube Transposase Enzyme Linked Long-read Sequencing (TELL-seq

171 Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, bu

172 P element transposase exhibits several unique properties, includin

173 distantly related elements with heteromeric transposases exist with alternate targeting pathways tha

174 ved 5 (PGBD5) gene as encoding an active DNA transposase expressed in the majority of childhood solid

175 Therefore, cells having residual transposase expression can be eliminated by the administ

176 alternative strategies to achieve transient transposase expression, and engineered refinements in th

177 nd to eliminate the cells that have residual transposase expression.

178 nation, but RAG also belongs to the RNH-type transposase family.

179 RAG1/RAG2 (RAG) recombinase, a domesticated transposase, for assembly of antigen receptor genes.

180 The transposase forms an asymmetric dimer in which the two c

181 Here we characterize a CRISPR-associated transposase from cyanobacteria Scytonema hofmanni (ShCAS

182 copy structures of Transib(4,5), a RAG1-like transposase from Helicoverpa zea, that capture the entir

183 e integration; however, using transposon and transposase from separate vectors circumvented this.

184 cies, as well as in humans, but with loss of transposase function (except Schizosaccharomyces japonic

185 antibody, which then tethers a protein A-Tn5 transposase fusion protein.

186 om mRNA, and SRTs deposited by exogenous, TF-transposase fusions can be used to map TFBS.

187 s1/2 (Rag1/2) recombinase has evolved from a transposase gene, demonstrating that TEs can be domestic

188 ding genes ccrA and ccrB, and the IS256-like transposase gene.

189 The high correlation between transposase genes and AMR genes, as well as plasmid repl

190 ely related to the activity of the TNP2-like transposase genes as the expression values of the transp

191 bovine isolates contained significantly more transposase genes but fewer transposase pseudogenes than

192 potheses for the enrichment of integrase and transposase genes in cryptic prophages.

193 that the different activity of the TNP2-like transposase genes is likely associated with the three in

194 -rich introns were retained in the TNP2-like transposase genes of the Bot1 (Brassica oleracea transpo

195 posase genes as the expression values of the transposase genes were higher in B. oleracea than in B.

196 is largely reflected by the activity of the transposase genes.

197 nogaster hsp70 promoter for a heat-inducible transposase helper plasmid, and creating vectors marked

198 ilarity between RAG1 and the hairpin-forming transposases Hermes and Tn5 suggests the evolutionary co

199 After electroporation, the transposon/transposase improves the efficiency of integration of pl

200 ically, accessible sites are captured by Tn5 transposase in permeabilized nuclei to permit, within ma

201 lements relationship parallels the MITEs/DNA-transposase in plants and SINEs/LINEs in mammals.

202 here the induction of expression of multiple transposases in a Streptococcus mitis biofilm when the p

203 eaction and the mechanism of hAT and Transib transposases including the importance of the conserved W

204 that several point mutations in the mariner transposase increase their activities by disrupting the

205 A popular example is ATAC-seq, whereby Tn5 transposase inserts sequencing adapters into accessible

206 nsposon greatly increased the probability of transposase integration; however, using transposon and t

207 aptations that transformed the ancestral RAG transposase into a RAG recombinase with appropriately re

208 a transgene flanked by binding sites for the transposase, into the cytoplasm of porcine zygotes.

209 e construct flanked by binding sites for the transposase, into the pronuclei of fertilized oocytes.

210 When purified recombinant Mos1 or Mboumar-9 transposase is co-transfected with transposon-containing

211 nsposition activity induced by the wild-type transposase is low but can be altered by modification of

212 2 and that in vitro transposition by Transib transposase is stimulated by RAG2.

213 ut our knowledge of human genes derived from transposases is limited.

214 ng the largest and most common among all DNA transposases, is the one whose members have been used fo

215 the structure and further show that the IS21 transposase, IstA, recognizes the IstB*DNA complex and p

216 In this study, we uncovered a domesticated transposase, Kluyveromyces lactis hobo/Activator/Tam3 (h

217 eta-catenin using sleeping beauty transposon/transposase leads to hepatocellular carcinoma (HCC) in m

218 rate that CRISPR/Cas9 combined with piggyBac transposase lineage labeling can produce unique models o

219 ore tested it in combination with a piggyBac transposase lineage labeling system to track the develop

220 e chromatin with visualization (ATAC-see), a transposase-mediated imaging technology that employs dir

221 efinements in the safety profile of piggyBac transposase-mediated integration.

222 ransduction efficiency of rAAV with piggyBac transposase-mediated somatic integration, was developed

223 The transposase-mediated transduction of constitutively acti

224 The transposase mediates excision of the transgene cassette

225 Otherwise, the transposase moiety behaved normally and was proficient f

226 A crystal structure of the mariner transposase Mos1 (derived from Drosophila mauritiana), i

227 mpared the preferences of two active mariner transposases, Mos1 and Mboumar-9, for their imperfect tr

228 Upon translation of the transposase mRNA, enzyme-mediated excision of the transg

229 e enhancer DNA segment is the site where the transposase MuA binds and makes bridging interactions wi

230 Finally, we identify transposase mutants that reveal that the conserved WVPHE

231 te and trithorax (SET) histone methylase and transposase nuclease domain.

232 ee-dimensional structures of transpososomes (transposase-nucleic acid complexes) are available, and w

233 f the transposon ends before cleavage by the transposase occurs.

234 ed sensitive biochemical assays for the TnpA transposase of the Tn3-family transposon Tn4430 and used

235 The TnpA transposase of these elements catalyzes DNA breakage and

236 Ac elements, these results give insight into transposase overproduction inhibition by demonstrating t

237 results were obtained with the domesticated transposase PogZ, another cellular interaction partner o

238 LPs) as carriers of the hyperactive piggyBac transposase protein (hyPBase), we demonstrate rates of D

239 We demonstrate lentiviral co-delivery of the transposase protein and vector RNA carrying the transpos

240 orientation in the genome and the amount of transposase protein in the cell.

241 tion by demonstrating that the appearance of transposase protein structures and the end of active tra

242 genetic material encoding the gene-inserting transposase protein, raising concerns related to persist

243 transposition achieved by direct delivery of transposase protein.

244 Analysis of transposase proteins containing site-directed mutations

245 eads to downstream recruitment of additional transposase proteins, and will guide protein engineering

246 gnificantly more transposase genes but fewer transposase pseudogenes than human isolates, suggesting

247 from a white eye mutant strain had an intact transposase reading frame and terminal sequences consist

248 ormation system by linking the hopper(Bd-we) transposase reading frame to a D. melanogaster hsp70 pro

249 Transposase recognises the flipped target adenines via b

250 in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluoresce

251 ccurs via overlapping inversions rather than transposase/recombinase-mediated processes.

252 nome editing agents-nucleases, base editors, transposases/recombinases and prime editors-are currentl

253 Transposase residues W159, R186, F187 and K190 stabilise

254 retained Har-P's that when crossed back to P-transposase restores GD induction.

255 ith guide RNA (gRNA), donor DNA and piggyBac transposase resulted in efficient, targeted genome editi

256 However, the 3'-end can be bypassed by the transposase, resulting in transduction of flanking seque

257 oncentrations became high enough for visible transposase rodlets to appear.

258 ow but can be altered by modification of the transposase sequence, including deletion, fusion, and su

259 a method that exploits contiguity preserving transposase sequencing (CPT-seq) to facilitate the scaff

260 ice et al suggest that the CRISPR-associated transposase ShCAST system could lead to additional inser

261 Analysis of their transposases shows that they contain a previously unchar

262 Activation of the transposase simultaneously cleaves DNA and adds adapters

263 atomic structure of the Drosophila P element transposase strand transfer complex using cryo-EM.

264 One transposase subunit, TnsB, is from the large family of b

265 Communication between transposase subunits also provides a failsafe mechanism

266 hofmanni (ShCAST) that consists of Tn7-like transposase subunits and the type V-K CRISPR effector (C

267 ure provides the first view of the P element transposase superfamily, offers new insights into P elem

268 ing transcriptional slippage is required for transposase synthesis.

269 ed using the Sleeping Beauty (SB) transposon/transposase system to express a CD19-specific CAR.

270 We used the Sleeping Beauty (SB) transposon/transposase system to identify activated oncogenes in he

271 assette according to the piggyBac transposon/transposase system.

272 e we show that the DNA-binding domain of the transposase targets the enzyme to transposon-end remnant

273 We found that the hAT transposase TcBuster from Tribolium castaneum formed fil

274 TnsB, is from the large family of bacterial transposases, the second, TnsA, is related to endonuclea

275 ase DNase I, and ATAC-seq, which is based on transposase Tn5, have been widely used to identify genom

276 es are then used to tether the cut-and-paste transposase Tn5.

277 w DNA binding is modulated by the asymmetric transposase to allow the capture of a second transposon

278 multiple sites of interaction could allow a transposase to locate its transposon ends amidst a sea o

279 ACT-seq utilizes a fusion of Tn5 transposase to Protein A that is targeted to chromatin b

280 ic underlying the transformation of RAG from transposase to recombinase.

281 ubstrate pair, Escherichia coli ClpX and MuA transposase, to address how these powerful enzymes recog

282 nthetic mRNA encoding the SB100X hyperactive transposase together with plasmid DNA carrying a transge

283 of a plasmid encoding the SB100X hyperactive transposase, together with a second plasmid carrying a t

284 nthetic mRNA encoding the SB100X hyperactive transposase, together with circular plasmid DNA carrying

285 such gene encodes the domesticated piggyBac transposase TPB6, required for heterochromatin-dependent

286 ilitated by one or more proteins, called the transposase, typically encoded by the mobile element its

287 mational changes in which the 'wings' of the transposase unfurl to bind substrate DNA, close to execu

288 xcised by a K248A excision(+)/integration(-) transposase variant are processed by hairpin resolution,

289 ers, fluorescent protein genes, and piggyBac transposase vectors to demonstrate that this can be a re

290 A hyperactive PB (hyPB) transposase was then deployed to enable transposition of

291 ures of wild-type and catalytically inactive transposases, we show that all the catalytic steps of tr

292 Chimeric transposases were evaluated for expression, transpositio

293 over, we identified a critical region in the transposase where the net charge of the amino acids seem

294 rast to strand transfer complexes of genuine transposases, where severe kinks occur at the integratio

295 (DDN) differs from the DDD motif of related transposases, which may be important for its role as a D

296 cular group of DNA transposons that encode a transposase with a DD(E/D) catalytic domain that is topo

297 We found that the FKBP-DD confers the PB transposase with a higher transposition activity and bet

298 We investigated fusions of the PB transposase with ERT2 and two degradation domains (FKBP-

299 vide a template for re-designing mariner/Tc1 transposases with modified target specificities.

300 The conserved mariner transposase WVPHEL and YSPDL motifs position the strand