ライフサイエンスコーパス: transposition

1 s transmitted paternally to suppress somatic transposition.

2 neration of long staggered DNA breaks during transposition.

3 uenesulfonylhydrazone with in situ reductive transposition.

4 ase in copy number due to periodic bursts of transposition.

5 d the characterization of key steps in Muta1 transposition.

6 facts are often mistaken for genuine somatic transposition.

7 reakage and rejoining reactions required for transposition.

8 gions that are otherwise poor targets for SB transposition.

9 d regio- and stereospecific substitutive 1,3-transposition.

10 must hijack the machinery required for their transposition.

11 recleaved ends mimicking the initial step of transposition.

12 Dsg requires the autonomous element Ac for transposition.

13 acquired during RAG domestication to inhibit transposition.

14 n reactions, such as V(D)J recombination and transposition.

15 tive malonate alkylation followed by C- to N-transposition.

16 then a Curtius rearrangement to provide the transposition.

17 erminal inverted repeat also influence Muta1 transposition.

18 nt locus indicate the origin and target of a transposition.

19 in 50+/-5% GFP-expressing cells after stable transposition.

20 al tool to unravel the mechanism of Helitron transposition.

21 ed in the C2C12 myoblasts that had undergone transposition.

22 h all of the domains required for autonomous transposition.

23 us COM1 strain and of in vitro Mariner-based transposition.

24 imes a selected insertion site for efficient transposition.

25 boumar-9 may be a general feature of mariner transposition.

26 omosomal DNA mutagenized heavily by in vitro transposition.

27 ature target site duplication of mariner/Tc1 transposition.

28 ISPR-Cas system for programmable, RNA-guided transposition.

29 g in target DNA and thus attenuates unwanted transposition.

30 with unique regioselectivity and no allylic transposition.

31 d, promote efficient and controllable alkene transposition.

32 AAA+ systems involved in DNA replication and transposition.

33 s are: substitution, insertion, deletion and transposition.

34 hed at tDNA targets with high frequencies of transposition.

35 encoded variants can guide sequence-specific transposition.

36 We found no evidence of retrograde transposition.

37 Remarkably, 25% of these were transpositions.

38 erage, and is particularly suited to mapping transpositions.

39 e tandem gene duplications and also probably transpositions.

40 s allow functionally distinct guide RNAs for transposition: a conventional system capable of acquirin

41 ns provided a fine source for analyzing MITE transposition activities and their impacts on genome evo

42 ith appropriately regulated DNA cleavage and transposition activities are not understood.

43 As the first study concentrated in the transposition activities of MITEs among different ecotyp

44 -DD confers the PB transposase with a higher transposition activity and better dynamic range than can

45 ion yet a comprehensive assessment of recent transposition activity at the species level is lacking f

46 The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic re

47 The transposition activity induced by the wild-type transpos

48 analysis of a series of TnsE mutants relates transposition activity to DNA binding stability.

49 transposases were evaluated for expression, transposition activity, chromatin immunoprecipitation at

50 cent protein to the transposase enhanced the transposition activity, representing another approach to

51 Using a transgenic reporter for RE transposition activity, we demonstrate increased transpo

52 ring RAG domestication led to suppression of transposition activity.

53 During transposition, an array of N-terminal domains binds a si

54 p22/p18 strongly inhibits transposition and can be considered an element-encoded r

55 Besides creating mutations via transposition and contributing to genome size, TEs play

56 Interestingly, transposition and deletion rates remain constant across

57 body diversification by interchromosomal DNA transposition and demonstrate the existence of conserved

58 findings suggest the existence of RNA-guided transposition and fit the guns-for-hire concept whereby

59 observed, but instead we found active (retro)transposition and gene family expansion, especially in p

60 erfamily, offers new insights into P element transposition and implies a transposition pathway fundam

61 In addition, yeast transposition and in vitro assays demonstrated that the

62 ariation between the amount of DNA gained by transposition and lost by deletion during mammal and avi

63 val and rapid proliferation: tissue-specific transposition and minimization of negative impacts on ne

64 g a new role for the WVPHEL motif in mariner transposition and providing a molecular explanation for

65 ting forces that oppose TE spread: regulated transposition and purifying selection.

66 Thus, understanding factors that limit transposition and retroviral replication is fundamentall

67 SB-mediated genetic transposition and stimulation resulted in 2,200- to 2,50

68 rinciples of the final step in cut-and-paste transposition and the molecular and structural logic und

69 mediated insertion pathway distinct from DNA transposition and trans-V(D)J recombination that destabi

70 /2 can also induce genomic DNA insertions by transposition and trans-V(D)J recombination, but only fe

71 human primates reveals a 746-kbp duplicative transposition and two separate inversion events that aro

72 against the reference sequence revealed 564 transpositions and 47 inversions comprising approximatel

73 ent of large-scale rearrangements, including transpositions and inversions.

74 ts provide mechanistic insight into Helitron transposition, and its impact on diversification of gene

75 transposon excision, host DNA recombination, transposition, and low-efficiency TIR rejoining using re

76 (MITEs) are preferred substrates of mariner transposition, and this process is associated with the e

77 ase protein structures and the end of active transposition are simultaneous, an effect with implicati

78 etween the two groups: lineage-specific gene transpositions are unusual in mammals, whereas single-co

79 rich mobilome can have and the importance of transposition as a recurrent generator of large-effect a

80 few Ty1 elements are present and inhibiting transposition as copy number increases.

81 ication and, to a lesser extent, single gene transposition as having played roles in the evolution of

82 ptase inhibitor 3TC resulted in decreased TE transposition as well as increased life span in TE-sensi

83 etrotransposons without the need for mapping transposition, as well as independent of genomic copy nu

84 nt with reduced activity as shown in a yeast transposition assay.

85 introduction of InDels via the method TRIAD (transposition-based random insertion and deletion mutage

86 precisely, we validated 83% of 44 predicted transposition breakpoints by polymerase chain reaction.

87 Thus, each transposition burst generates a novel progeny population

88 cumulation in plant populations experiencing transposition bursts accompanied by high diversity of ch

89 anisms, of which the first prevents multiple transposition bursts in a given somatic cell lineage tha

90 that relaxed purifying selection rather than transposition bursts is the main driver of TE over-accum

91 Here, we studied transposition bursts of a heat-activated retrotransposon

92 he amount of DNA gained via lineage-specific transposition, but that DNA loss counteracted this expan

93 ing Cas3 have been repurposed for RNA-guided transposition by bacterial Tn7-like transposons(6,7).

94 Radical transposition by C(sp(3) )-(sp(3) ) and C(sp(3) )-H bond

95 tiple times in nature for guide RNA-directed transposition by Tn7-like elements.

96 when coexpressed with RAG2 and that in vitro transposition by Transib transposase is stimulated by RA

97 ShCAST catalyzes RNA-guided DNA transposition by unidirectionally inserting segments of

98 The timing of past transposition can be estimated by quantifying the accumu

99 citing reactivity patterns like 1,3-carbonyl transpositions, carbene transfer reactions, cascade annu

100 on-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in Dros

101 In Drosophila, P-element transposition causes mutagenesis and genome instability

102 study nicely illustrates how the presence of transposition-competitive variants can deeply impair TE

103 pect natural assumptions of invariance under transposition, convexity, and contractivity under quantu

104 SINE_Scan integrates hallmark of SINE transposition, copy number and structural signals to ide

105 Extant genomes are largely shaped by global transposition, copy-number fluctuation, and rearrangemen

106 psis that old members enter into replication/transposition cycles through high rates of intra-family

107 ability of the transposon DNA and found that transposition declined after transposase concentrations

108 During Pisum evolution, translocation and transposition differentially occurred across lineages.

109 eir imperfect transposon IRs in each step of transposition: DNA binding, DNA cleavage, and DNA strand

110 Here, we describe the transposition dynamics of cut-and-paste mariner elements

111 Finally, transposition efficiency is also influenced by the eleme

112 e fold-induction achieved, (ii) the absolute transposition efficiency of the activated construct and

113 Transposition efficiency was first tested using an enhan

114 ed carbonaria haplotypes indicates that this transposition event occurred around 1819, consistent wit

115 s neither shortened nor lengthened with each transposition event.

116 able for the control it exercises over where transposition events are directed.

117 ymorphic TEs include a combination of recent transposition events as well as deletions of older TEs.

118 d lineage-specific clusters that derive from transposition events for the regulators of floral develo

119 ions, we found that frameshift mutations and transposition events increased in the progeny of older p

120 s the globe, we identify thousands of recent transposition events involving half of the 326 TE famili

121 ectious particles that can lead to heritable transposition events.

122 This requires that each substrate transposition exposes a catalyst activation mode (AM) to

123 t combinations required for large changes in transposition frequency and targeting stabilized this in

124 A new study provides evidence that gene transposition from sex chromosomes to autosomes is a con

125 osome has an expanded number of genes due to transpositions from autosomes.

126 Helraiser transposition generates covalently closed circular inter

127 Superior rectus transposition has been popularized for the treatment of

128 onine 848 explain how this residue activates transposition, how RAG can stabilize sharp bends in targ

129 echanism for the suppression of RAG-mediated transposition, illuminate the evolution of V(D)J recombi

130 nowledge, the first demonstrations of intron transposition in any organism.

131 DNA substrates and its inability to perform transposition in cells.

132 corresponding age-associated increase in TE transposition in fly fat body cells that was delayed by

133 es, suggesting the occurrence of large-scale transposition in genomes of bovine and human isolates at

134 We present a stochastic model of LINE-1 (L1) transposition in human cancer.

135 , can induce stereotypical cut-and-paste DNA transposition in human cells.

136 Somatic transposition in mammals and insects could increase cell

137 genomic data revealed no signature of higher transposition in natural hybrids.

138 as mechanisms for site-specific programmable transposition in prokaryotic organisms.

139 To understand the impact of transposition in somatic cells it is essential to reliab

140 sposition activity, we demonstrate increased transposition in the dnmt1(-/-) CMZ.

141 red for efficient transposon integration and transposition in vitro.

142 lement from Aedes aegypti and its successful transposition in yeast facilitated the characterization

143 e could not rule out the elimination of past transposition increase signatures by natural selection,

144 However, certain transposition-induced gain-of-function or regulatory mut

145 The analysis of 278 transposition-induced prostate, breast and skin tumors d

146 facilitates horizontal transfer by directing transposition into actively replicating DNA with the ele

147 ite in the chromosome and a second directing transposition into mobile plasmids facilitating cell-to-

148 o a mammalian-wide interspersed repeat (MIR) transposition into the 5' leader sequence of the mammali

149 marker to follow repair, we find that after transposition into the chromosome, the unrepaired Mu is

150 ted to use guide RNAs to specifically direct transposition into these mobile elements.

151 sition (IORAT) and Inferior Oblique Anterior Transposition (IOAT) in the management of unilateral Sup

152 Inferior Oblique combined Resection-Anterior Transposition (IORAT) and Inferior Oblique Anterior Tran

153 A new procedure, the inferior rectus transposition (IRT), may be similarly beneficial for pat

154 Copy-out/paste-in transposition is a major bacterial DNA mobility pathway.

155 Our findings show that piggyBac transposition is a more efficient approach than the clas

156 As are self-propagating, and their continued transposition is a source of both heritable structural v

157 Superior wide-base ILM flap transposition is a useful technique for the management o

158 tial analysis of new insertions suggest that transposition is biased to proximal insertions, and the

159 The data suggest that transposition is controlled at an early stage of transpo

160 We show that the efficiency of Mboumar-9 transposition is improved almost 4-fold by changing the

161 To learn how RAG-catalyzed transposition is inhibited in developing lymphocytes, we

162 aryotes, but experimental evidence for their transposition is lacking in the absence of an isolated a

163 We conclude that somatic transposition is less prevalent in Drosophila than previ

164 This burst of transposition is likely related to the activity of the T

165 For both Mos1 and Mboumar-9, in vitro DNA transposition is most efficient when the preferred IR se

166 for many experiments it is important that PB transposition is tightly controlled.

167 ovalently joined to target DNA, portrays the transposition machinery after DNA integration.

168 autonomous mariner elements by hijacking the transposition machinery produced by active mariner, whic

169 es the foundation for further studies of the transposition mechanism of MULEs.

170 d importance as genetic tools in plants, the transposition mechanism of the MULE superfamily was prev

171 r intermediates, suggestive of a replicative transposition mechanism, which provides a powerful means

172 and biological significance as well as their transposition mechanisms are poorly understood.

173 in RAG2-that together suppress RAG-mediated transposition more than 1,000-fold.

174 es have autosomal paralogs, so autosome-to-Y transposition must be the main source of Drosophila Y-li

175 Transposition mutagenesis is a powerful tool to identify

176 This transposition mutagenesis strategy will greatly facilita

177 The structure of this post-transposition nucleoprotein complex reveals that the ter

178 ses, we show that all the catalytic steps of transposition occur within the context of a dimeric tran

179 is well-mixed and uncompartmentalized, with transpositions occurring freely between all measured loc

180 abeling experiments disclose that the oxygen transposition occurs from the carboxylate group to the t

181 cation mechanism, it was recently shown that transposition of a bat Helitron generates covalently clo

182 We find that all are produced by transposition of a defective LTR retrotransposon that we

183 vealed that the Abph2 mutation was caused by transposition of a glutaredoxin gene, MALE STERILE CONVE

184 ron gain events characterized by the perfect transposition of a reporter intron into the yeast genes

185 t full details of a method for 1,3-reductive transposition of alpha-alkoxy-alpha,beta-unsaturated hyd

186 The transposition of bacteriophage Mu serves as a model syst

187 A recent burst of transposition of Bot1 was observed in B. oleracea, but n

188 in to be clarified but likely ensue from the transposition of center of mass of the ion within its ge

189 With the recent transposition of Directive 2010/53/EU into the transplan

190 is raises the possibility that a genome-wide transposition of ERVs rewired germline gene expression i

191 angements, local nucleotide changes, and the transposition of insertion sequences.

192 he mobilisation of bla CMY-42 was due to the transposition of ISEcp1 by misidentifying its right-end

193 This system was chosen because the transposition of L1 elements is well understood, the pop

194 t yeast harbors all the host factors for the transposition of MULEs.

195 ly, we detected both the deletion and/or the transposition of multicopy TEs associated with 2b-induce

196 del that transcription results in retrograde transposition of nucleosomes along a transcription unit.

197 Transposition of Ping16A into a Stowaway element generat

198 We report that a regioselective reductive transposition of primary allylic bromides is catalyzed b

199 ng of the retinal projections and concurrent transposition of retinal guidance cues into the superior

200 Our data suggest that transposition of selfish DNA, low effective population s

201 ocess features regio- and stereospecific 1,3-transposition of the allyl fragment enabled by an aromat

202 CHD, such as single ventricle (22.8%) and d-transposition of the great arteries (22.1%).

203 sis and management of congenitally corrected transposition of the great arteries (ccTGA).

204 lation and compared with control groups with transposition of the great arteries (n=50) and a normal

205 that of 15 fetuses with major heart defects: transposition of the great arteries (n=7), coarctation o

206 Patients with transposition of the great arteries (TGA) and systemic r

207 tle has been published regarding surgery for transposition of the great arteries (TGA) in the develop

208 haemodynamic effects of exercise training in transposition of the great arteries (TGA) patients with

209 n, the Mustard or Senning operation, for the transposition of the great arteries (TGA) was introduced

210 th hypoplastic left heart syndrome (HLHS) or transposition of the great arteries (TGA), diagnoses wit

211 operation and 2 by aortopulmonary shunts: d-transposition of the great arteries after Mustard/Sennin

212 Among 153 patients (67% male), 96 had transposition of the great arteries and 57 had single ve

213 cles (RVs), including congenitally corrected transposition of the great arteries and dextro-transposi

214 ocognitive function compared with those with transposition of the great arteries and healthy controls

215 A total of 153 patients with transposition of the great arteries and single ventricle

216 ved for the CNVs identified in patients with transposition of the great arteries and tetralogy of Fal

217 slower language development in infants with transposition of the great arteries cared for at our cen

218 ular septal defect, tetralogy of Fallot, and transposition of the great arteries decreased to zero fo

219 diagnosis of single ventricle physiology and transposition of the great arteries demonstrate less pre

220 Only infants with transposition of the great arteries had smaller HC relat

221 e novo CNVs were identified in patients with transposition of the great arteries in contrast to coarc

222 resonance predicts outcomes in patients with transposition of the great arteries post atrial redirect

223 The brains of 45 infants with transposition of the great arteries undergoing surgical

224 injury and poor brain growth in infants with transposition of the great arteries undergoing the arter

225 Transposition of the great arteries was associated with

226 ght mothers, PRRs of aortic arch defects and transposition of the great arteries were doubled in offs

227 ansposition of the great arteries and dextro-transposition of the great arteries with a Mustard or Se

228 , including 76 coarctation of the aorta, 159 transposition of the great arteries, and 81 tetralogy of

229 complex heart defects (tetralogy of Fallot, transposition of the great arteries, atrial septal defec

230 TPVR in patients with congenitally corrected transposition of the great arteries, subpulmonary left v

231 Other single-ventricle defects, transposition of the great arteries, tetralogy of Fallot

232 to double-outlet right ventricle and dextro-transposition of the great arteries.

233 pmental problems are common in children with transposition of the great arteries.

234 tralogy of Fallot, coarctation of the aorta, transposition of the great vessels, hypoplastic left hea

235 tandem annulation is proposed to proceed via transposition of the hydroxy group resulting from the in

236 iphenols, results in an asymmetric reductive transposition of the in situ generated allylic diazene.

237 yPB) transposase was then deployed to enable transposition of the large-size PB transposon (17 kb) en

238 ISApl1 was presumably involved in the transposition of the mcr-1 cassette and then was lost.

239 erties of rAAV with stable piggyBac-mediated transposition of the transgene into the hepatocyte genom

240 sisted of either a 7-mm or full tendon-width transposition of the vertical rectus muscles of each eye

241 e report 5 patients who underwent horizontal transposition of the vertical rectus muscles to correct

242 Transposition of the vertical rectus to induce cyclotors

243 Programmable transposition of Vibrio cholerae Tn6677 in Escherichia c

244 autonomous retrotransposons and very recent transpositions of a TRIM element in Oryza sativa.

245 Oxidative transpositions of bicyclic cyclopentenones mediated by s

246 ng the substitution, insertion, deletion and transposition operations.

247 or acquired by horizontal gene transfer via transposition or homologous recombination.

248 These gene transpositions originated from either the X or Y chromos

249 s into P element transposition and implies a transposition pathway fundamentally distinct from other

250 The transposition pathway resembles that of the V(D)J recomb

251 Unlike other transposition pathways, it requires both asymmetric and

252 DNA transposition plays key roles in genome diversity, patho

253 e silencing of active elements and influence transposition potential, siRNA targeting levels, and ult

254 operative hypertropia or intorsion requiring transposition procedures.

255 rom Helicoverpa zea, that capture the entire transposition process from the apo enzyme to the termina

256 improve our understanding of their origins, transposition processes, regulatory mechanisms, and biol

257 dopsis thaliana we show that once initiated, transposition produces an exponential spread of TE copie

258 We demonstrate that the final transposition product, which is a DNA-bound tetramer of

259 bled de novo modelling and refinement of the transposition protein TniQ, which binds to the Cascade c

260 en the DNA-targeting complex Cascade and the transposition protein TniQ.

261 ly invasive and characterized by an elevated transposition rate, confirming their capacity to spread

262 A small-scale transposition reaction routinely generates several hundr

263 tructures of these enzymes at the end of the transposition reaction, including Mu, exhibit significan

264 eir oxazole counterparts via a photochemical transposition reaction.

265 e ramifications of target DNA bending on the transposition reaction.

266 em aldol cyclizations, and one-pot reduction/transposition reactions allowing a rapid (7 linear steps

267 RNA-cDNA molecule is required for mutagenic transposition, revealing a unique mechanism of DNA hyper

268 nt involving a sulfanyl elimination-addition transposition sequence.

269 e method that we call "contiguity preserving transposition" sequencing on beads (CPTv2-seq) is transp

270 Saturation transposition showed that tRNA genes (tDNAs) are targete

271 We illustrate several examples of mammalian transpositions, such as brain-development genes in prima

272 of morning glory disc anomaly and therefore transposition surgery was only performed on fixating eye

273 MH surgery with superior wide-base ILM flap transposition (SWIFT).

274 ession systems and the PiggyBac-mediated DNA transposition system in avian embryos.

275 er, including a loop that interacts with the transposition target site and an accordion-like C-termin

276 cluding Mu, exhibit significant bends in the transposition target site DNA.

277 t, structural and mechanistic information on transposition targeting and regulation is limited.

278 ential effects of cis-acting DNA features on transposition, targeting was tested on high-copy plasmid

279 ses to the environment are the most frequent transposition targets observed.

280 the 3'-end of the transposon functions as a transposition terminator.

281 detoxification, replication, recombination, transposition, tethering, and transport.

282 predicted to change during the final step of transposition, this recognition strategy suggests how AA

283 ology, was subjected to a reductive carbonyl transposition to afford 8-(4-methoxyphenyl)perinaphthano

284 Rescue of Y-linked gene loss through transposition to autosomes has previously been reported

285 equence-independent structures maintained by transposition to chromosome ends of three specialized re

286 ation of saturated target sites by promoting transposition to different target sites.

287 The approach utilizes in vitro transposition to generate 5 and 3 fragment sub-libraries

288 We used in vitro Mu transposition to generate a random truncation library an

289 ure on nonhuman species, we investigated the transposition to ionizing radiation ecotoxicity of one m

290 ich renders TSPX to be stimulatory while its transposition to the C-terminus of TSPY results in an in

291 he reaction pathway, whether the double bond transposition toward the conjugated 1,3-diene or the all

292 During cut-and-paste mariner/Tc1 transposition, transposon DNA is cut precisely at its ju

293 that this "innate" piRNA response suppresses transposition until antisense piRNAs are produced, estab

294 The efficiency and promiscuity of phage Mu transposition was exploited to directly measure in vivo

295 in a Mendelian ratio of 3:1 and no new Tnt1 transposition was observed.

296 Using transgenes and transpositions, we show that most endogenous X-linked ge

297 Transpositions were also detected, revealing replicon pr

298 In contrast to mariner transposition, where a topological filter suppresses sin

299 s (inversions, reciprocal translocations and transpositions), whereas S. cerevisiae accumulates unbal

300 of these variants are involved in RNA-guided transposition, whereas others are predicted to perform f