ライフサイエンスコーパス: trypsin

1 ing molecular mass (melamine, vancomycin and trypsin).

2 Na(+) ion next to Asp189, which is absent in trypsin.

3 ties with plant cyclotides that also inhibit trypsin.

4 c model and from solutions of intact BSA and trypsin.

5 -l-phenylalanine chloromethyl ketone-treated trypsin.

6 ypsinogen sensitive to CTSB but resistant to trypsin.

7 and human cells in the presence of exogenous trypsin.

8 thereby preventing accumulation of activated trypsin.

9 ified in camel milk proteins hydrolysed with trypsin.

10 ydrolysis of pressure-treated proteins using trypsin.

11 mpared it to the gold standard in the field, trypsin.

12 essed, BBI3, encodes a protein that inhibits trypsin.

13 behaves more similarly to subtilisin than to trypsin.

14 onspecific digestion of unfolded peptides by trypsin.

15 ter digesting the precipitated proteins with trypsin.

16 in, which is replaced by uncharged Gln192 in trypsin.

17 the modified protein was then digested with trypsin.

18 nactive against the off-targets thrombin and trypsin.

19 Western corn rootworm (WCRW) midgut juice or trypsin, 100 fg/ml of the toxin was sufficient to form p

20 egrees C for 15 minutes or pretreatment with trypsin abolished TG, suggesting the presence of MV-asso

21 vels, are fully protected from intracellular trypsin accumulation and acinar damage.

22 without directly affecting intra-acinar cell trypsin activation in vitro The absence of CTSC reduced

23 t Asn(30) Arg(31), proximal to the canonical trypsin activation site.

24 mitochondrial depolarization, intracellular trypsin activation, and cell death.

25 ymotrypsin activation and a trend for higher trypsin activation, compared with C57BL/6N mice.

26 hat CTRL cleaved trypsinogens and suppressed trypsin activation.

27 as well as cathepsin B, myeloperoxidase and trypsin activities were determined throughout the first

28 us spectrophotometric rate determination for trypsin activity against the substrate N-benzoyl-DL-argi

29 Drain fluid stabilized trypsin activity and prevented the activation of the cas

30 everity of pancreatitis, even though loss of trypsin activity does not prevent pancreatitis in mice.

31 ultrasonic assay provided rapid detection of trypsin activity even at sub-nanomolar concentration.

32 PRSS1R122H mice, we unraveled that increased trypsin activity is the mechanism for R122H mutation to

33 Trypsin activity, cathepsin B activity and myeloperoxida

34 , acinar cell vacuolization, intrapancreatic trypsin activity, cell death signalling and acinar cell

35 own-regulation of digestive enzyme genes and trypsin activity, upon exposure to Tsp_PR-secreted facto

36 eas against pancreatitis by reducing harmful trypsin activity.

37 se activity, including specific increases in trypsin activity.

38 atitis through regulation of intrapancreatic trypsin activity.

39 cer therapy, especially for tumors with high trypsin activity.

40 is study, three proteolytic enzymes (pepsin, trypsin, Alcalase(R)) were used, alone or combined, to p

41 protein identifications than digesting with trypsin alone.

42 Protein removal with trypsin also reduced the rate of mineralization, but to

43 lly, we found that the addition of exogenous trypsin also rescues HKU5-CoV, a second bat group 2c CoV

44 After 72 h at 40 degrees C, only trypsin and alpha-amylase maintained high activity.

45 Plasma samples were digested with trypsin and analyzed with liquid chromatography-tandem m

46 Interestingly, the sequential digest with trypsin and AspN yielded even a higher number of protein

47 been discussed as central to the binding of trypsin and BPTI and protein complex formation in genera

48 nge interactions because opposite charges in trypsin and BPTI draw them together.

49 ters of milk samples have been digested with trypsin and chymotrypsin and analyzed by nanoLC-ESI-IT-M

50 Then, they are affected by trypsin and chymotrypsin as indicator intestinal enzymes

51 digestibility was exerted by the activity of trypsin and chymotrypsin inhibitors.

52 Also, the extent of hydrolysis by trypsin and chymotrypsin of ProS were higher and faster

53 of a hydrophilic gel, previously loaded with trypsin and chymotrypsin proteolytic enzymes, was succes

54 p of FVIIa with the more rigid 170 loop from trypsin and combined it with an L163V substitution (FVII

55 port on a sequential-digestion strategy with trypsin and elastase to penetrate regions with a low den

56 he combination of three enzymes (subtilisin, trypsin and flavourzyme) employed sequentially.

57 explore the impacts of inhibitor binding to trypsin and FVIIa, as well as cofactor binding to FVIIa.

58 (P<0.05) antioxidant activities compared to trypsin and HT, while trypsin and pepsin hydrolysates ex

59 il with molecular dynamics simulations using trypsin and its inhibitor bovine pancreatic trypsin inhi

60 Mutations in trypsin and other genes thought to be associated with pa

61 activities compared to trypsin and HT, while trypsin and pepsin hydrolysates exhibited higher ACE-inh

62 ACE-inhibitory activity was generated in the trypsin and pepsin hydrolysates than in the HT hydrolysa

63 The enzymes trypsin and pepsin were immobilized on amino-functionali

64 ers, the membrane proteins are digested with trypsin and quantitated by parallel reaction monitoring

65 of IGNIS prime peptides was optimised using trypsin and SMART Digest.

66 Trypsin and thrombin, structurally similar serine protea

67 actions at the specificity binding pocket of trypsin and trypsin-like proteins from Serine-protease d

68 ually from cultured embryos dissociated with trypsin and were able to follow sublineages that emerged

69 tins or protease inhibitors (chymotrysin and trypsin) and 12 polyphenol compounds were identified in

70 ated by serine proteinases such as thrombin, trypsin, and cathepsin-G.

71 ptor N terminus by enzymes such as thrombin, trypsin, and cathepsin-G.

72 -beta (TGF-beta), serine proteinases such as trypsin, and proteinase-activated receptor 2 (PAR2) prom

73 depletion of albumin, protein digestion with trypsin, and stabilization of tryptic peptides on solid-

74 approach, 6aJL2 protein was proteolyzed with trypsin, and the products incubated in aggregation-promo

75 sociated with the proteasome (chymotrypsin-, trypsin-, and caspase-like) in the superior temporal gyr

76 binding-competent conformations of BPTI and trypsin are already present in the apo ensembles and the

77 As human trypsins are post-translationally sulfated in the autoly

78 on of bovine serum albumin (BSA) digested by trypsin as an enzymatic model and from solutions of inta

79 as prepared and subsequently hydrolysed with trypsin at different temperatures, enzyme to substrate (

80 time monitoring of beta-casein hydrolysis by trypsin at various conditions for the first time.

81 pensive, simple and fast procedure to purify trypsin based on affinity binding with ferromagnetic par

82 The new trypsin-based workflow was easily implemented by adaptin

83 cation of the wheat extract with immobilized trypsin beads led to the identification of CM inhibitors

84 lations of full ligand exit pathways for the trypsin-benzamidine system, generated using the sampling

85 While trypsin binding is mediated by an ordered water molecule

86 on previous work demonstrating that purified trypsin can activate NanI activity, this study next dete

87 Our results suggest that trypsin can be stabilized by a combination of calcium io

88 ted for digestion using a ((3)D) immobilized trypsin cartridge and, finally, the obtained peptides we

89 phagic vacuoles and activation of proteases (trypsin, chymotrypsin).

90 The water-holding capacity and trypsin-chymotrypsin digestibility of the proteins decre

91 cted their putative inhibitory activities to trypsin/chymotrypsin/elastase-like enzymes based on the

92 emnant of ubiquitin on modified lysine after trypsin cleavage and therefore enables the generation of

93 Both MAbs increased HA sensitivity to trypsin cleavage at a higher pH range, suggesting MAb-in

94 The truncated protein is generated by trypsin cleavage of soluble HttEx1 fusion protein, which

95 ealed in all cases the existence of a unique trypsin cleavage site within the membrane domain (out of

96 aphic structure of the BMV capsid shows four trypsin cleavage sites (K(65), R(103), K(111), and K(165

97 e to penetrate regions with a low density of trypsin-cleavage sites.

98 strates; thrombin cleaves after Arg, whereas trypsin cleaves after Lys/Arg.

99 The X-ray structure of the ToPI1:trypsin complex, in association with the mass spectromet

100 tion conditions (buffer, digestion time, and trypsin concentration), chromatographic separation, and

101 mental parameters, such as tissue thickness, trypsin concentration, and enzyme incubation duration, w

102 d from combined digestion using OpeRATOR and trypsin contain multiple O-glycosites, indicating that c

103 We found here that trypsin, CS, and NE stimulate PAR(2)-dependent activatio

104 Trypsin-decorated magnetic beads (MBs, 1.5 mum) were muc

105 scopy, corresponding to a pre-equilibrium of trypsin deprotonation.

106 ication, providing a precision comparable to trypsin-despite broad specificity and fast digestion tha

107 nted in the direct analysis of peptides from trypsin digested bovine serum albumin.

108 robes was elucidated based on MS analysis of trypsin digested hABHD6 following the Ligand Assisted Pr

109 Affinity Chromatography (IMAC), in-solution trypsin digested, and proteomically characterized by Mat

110 MS in a simple and efficient way; LC-MS of a trypsin-digested bovine serum albumin (BSA) sample provi

111 ypsin digestion; three surrogate peptides in trypsin-digested human colon tissue homogenates; and a s

112 Retinopathy was detected in trypsin-digested microvasculature.

113 of individual peptides and full proteins on trypsin-digested monoclonal antibody samples (rituximab

114 protein N-termini not accessible in GluC- or trypsin-digested samples.

115 Our approach included the following steps: trypsin-digested tumor samples were enriched for phospho

116 The limited trypsin digestion (LTD) method has been developed and do

117 g a novel sample preparation procedure using trypsin digestion and a shotgun proteomics approach.

118 ne SIL peptide for each analyte, followed by trypsin digestion and antipeptide immunocapture enrichme

119 ted and unmodified lysine residues following trypsin digestion and secondary stable isotopic labeling

120 ion or amino acid racemization inhibition of trypsin digestion appears to limit greater recovery.

121 Together with the trypsin digestion based LC-MS/MS analysis using surrogat

122 ime of flight (MALDI-TOF) analyses following trypsin digestion of the three virions assembled separat

123 Following in silico trypsin digestion to ascertain theoretical core peptides

124 based on the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by

125 arginine such that each peptide derived from trypsin digestion was labelled.

126 onic bath for digestion enhancement, on-bead trypsin digestion was optimized to obtain efficient and

127 ucted peptide pairs based on chymotrypsin or trypsin digestion were developed, and the data were comp

128 is including two-dimensional gel separation, trypsin digestion, and nanospray liquid chromatography c

129 Prior to trypsin digestion, IgG and IgA were enriched simultaneou

130 rial, added as an internal standard prior to trypsin digestion, produces a stable-isotope-labeled sta

131 After trypsin digestion, the types and sites of modifications

132 d, solvent-accessible protein regions resist trypsin digestion, whereas hidden interface regions are

133 Thus, peptides resulting from trypsin digestion, with arginine (Arg) or lysine (Lys) a

134 were actually not identifiable using current trypsin digestion-based methods due to their inappropria

135 after immunocapture enrichment, followed by trypsin digestion.

136 nutes compared to 24 hours using in-solution trypsin digestion.

137 and subjected to alkali treatment or limited trypsin digestion.

138 onkey serum processed with immunocapture and trypsin digestion; three surrogate peptides in trypsin-d

139 ver, as it also inhibits the serine protease trypsin due to replacement of the P1 Leu(15) in the reac

140 Trypsinogen can be activated to trypsin either through autoactivation (trypsin-mediated

141 Subsequently, the trypsin enzyme was immobilized on the NAA pore walls.

142 ter digesting of cytochrome c by immobilized trypsin enzymes on NAA-NH(2) into the heme-peptide fragm

143 site influences the level of activity in the trypsin fold and defines ligand binding according to the

144 inkage with the allosteric properties of the trypsin fold like Na(+) binding and the E*-E equilibrium

145 ar basis of a key allosteric property of the trypsin fold.

146 e and the allosteric E*-E equilibrium of the trypsin fold.

147 By using chymotrypsin, trypsin, or trypsin followed by chymotrypsin to digest denatured cer

148 ysis, the remaining samples were digested by trypsin, followed by SRM detection.

149 acteristics of five protease alternatives to trypsin for protein identification and sequence coverage

150 avage relative to canonical proteases (e.g., trypsin) for improved O-glycopeptide characterization wi

151 tionation or enrichment; instead, we utilize trypsin-friendly sodium deoxycholate (SDC) as an advanta

152 omposite can be reused and applied to purify trypsin from other sources.

153 Unlike its constitutively active ancestor trypsin, FVIIa is allosterically activated (by TF).

154 predicting spectra for proteases other than trypsin, generating spectral libraries for data-independ

155 that binding of the inhibitor benzamidine to trypsin has a minor influence on the protease flexibilit

156 enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been

157 d with those produced from commercial bovine trypsin (HB).

158 nine chains of affibody could be digested by trypsin, helping to release MTX from MAMA.

159 ed: encapsulated peptides are protected from trypsin hydrolysis, whereas physicochemically similar pe

160 ysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of

161 In the most extreme case, predigesting with trypsin improves the number of identified proteins for p

162 Surprisingly, one ligand binds to trypsin in protonated state and to thrombin in unprotona

163 e K largely benefited from being paired with trypsin in sequential digestion, as had been shown by us

164 Notably, the prominent role of trypsins in the hydrolytic degradation of major allergen

165 Active trypsin increases permeability of these organelles resul

166 Higher pancreatic activity of trypsin increases the severity of pancreatitis, even tho

167 Whereas trypsin induces PAR(2) coupling to Galpha(q), Galpha(s),

168 CER-based protein extract resulted in higher trypsin inhibiting activity, when compared to the indust

169 an enzymatic assay for the determination of trypsin inhibition activity in hexaploid wheat was devel

170 e satisfactory for accurate determination of trypsin inhibition in wheat.

171 We further discovered that trypsin inhibition, in combination with anticoagulation

172 ded to mutate two lysine residues to abolish trypsin inhibition, suggesting BBI3's mechanism of doubl

173 ol soluble proteins) as main contributors of trypsin inhibition.

174 mparison to APIc and marked reduction in the trypsin inhibitor (85.97%) and lectin activity (100%) wa

175 trypsin and its inhibitor bovine pancreatic trypsin inhibitor (BPTI) as a model system.

176 e inhibitors of the Kunitz-bovine pancreatic trypsin inhibitor (BPTI) family are ubiquitous biologica

177 taining copy number variations of the Kunitz trypsin inhibitor (KTI) genes.

178 The determination of trypsin inhibitor (TI) activity is of importance to eval

179 sing a ladder of small-to-mid mass proteins (Trypsin Inhibitor (TI); Ovalbumin (OVA); Bovine Serum Al

180 es to the disulfide-linked 14-mer, sunflower trypsin inhibitor 1 are demonstrated.

181 esis of a functional analog of the Sunflower Trypsin Inhibitor 1.

182 Trypsin inhibitor activity (TIA) was evaluated using a s

183 r the first time a novel activity of soybean trypsin inhibitor and bovine aprotinin that they nick su

184 Dual inhibition of FXIIa by corn trypsin inhibitor and kallikrein by soybean trypsin inhi

185 We illustrate them using bovine pancreatic trypsin inhibitor and present a new, detailed analysis o

186 Indian hedgehog protein], ITIH4 [inter-alpha-trypsin inhibitor chain H4], SAA2 [serum amyloid A2], AP

187 annexin A1 protein grafted into a sunflower trypsin inhibitor cyclic scaffold.

188 A potent trypsin inhibitor from Tityus obscurus scorpion venom wa

189 % of these individuals have mutations of the trypsin inhibitor gene (SPINK1) or the cystic fibrosis t

190 Blockade of FXII by corn trypsin inhibitor had a significant inhibitory effect on

191 fer of heavy-chain proteins from inter-alpha-trypsin inhibitor to hyaluronan to form heavy-chain prot

192 trypsin inhibitor and kallikrein by soybean trypsin inhibitor was necessary for abolishing RBC-MV-in

193 rresponding to soybean agglutinin and Kunitz trypsin inhibitor were identified based on the statistic

194 acid, tannins, flavonoids, HCN, oxalate and trypsin inhibitor which were observed in the range of 99

195 igested beta-casein poorly and bound soybean trypsin inhibitor with 10-fold decreased affinity.

196 Protease inhibitors, such as trypsin inhibitor, serum alpha-1 antitrypsin, or liver a

197 the nature-derived cyclic peptide sunflower trypsin inhibitor-1 (SFTI-1) as a template for designing

198 Sunflower trypsin inhibitor-1 (SFTI-1) is a 14-amino acid cyclic p

199 thepsin G substrate sequences into sunflower trypsin inhibitor-1 (SFTI-1) produced a potent cathepsin

200 the 14-amino acid backbone-cyclic sunflower trypsin inhibitor-1 scaffold to design a highly potent (

201 s) into the cyclic peptide SFTI-1 (sunflower trypsin inhibitor-1) and a heterodimeric 2S albumin.

202 s annuus PawS1 (preproalbumin with sunflower trypsin inhibitor-1) and provide new insights into the p

203 The smaller C8-3, TIL3 (trypsin inhibitor-like 3), and E3 modules pack through s

204 mon dietary protein component, wheat amylase trypsin inhibitors (ATI), stimulate intestinal macrophag

205 BACKGROUND & AIMS: Wheat amylase-trypsin inhibitors (ATIs) are nutritional activators of

206 Amylase-trypsin inhibitors (ATIs) have recently been identified

207 Amylase-trypsin inhibitors (ATIs) in wheat and related cereals a

208 Amylase trypsin inhibitors (ATIs), a component of wheat, activat

209 eat or gluten, with or without wheat amylase trypsin inhibitors (ATIs), for 1 week.

210 ose, verbascose), inositol phosphates (IPs), trypsin inhibitors and lectins content.

211 ibitor resistance and the ability to degrade trypsin inhibitors.

212 F, FS and FSF samples; highest reduction in trypsin inhibitory activity (58%) was observed in the FS

213 tivity, ACE-, alpha-glucosidase-, and Kunitz trypsin-inhibitory activity.

214 eae) families, but peptides that mimic their trypsin-inhibitory loops exist in sunflowers (Helianthus

215 Intra-pancreatic activation of trypsin is an early event in pancreatitis.

216 n containing 10 uM myoglobin and 5 ug mL(-1) trypsin is electrosonically sprayed (-3 kV) from a homem

217 (SDS), at high temperature, conditions where trypsin is normally inactivated.

218 Trypsin is the most used enzyme in proteomics.

219 Mesotrypsin is an unusual human trypsin isoform with inhibitor resistance and the abilit

220 Six adduction sites were identified by trypsin (K20, C34, K73, K281, H338, K378) or chymotrypsi

221 LA3B, which upon secretion and activation by trypsin leads to uncontrolled proteolysis and recurrent

222 ecially in pancreatic cancer due to its high trypsin level.

223 SZ subjects had decreased trypsin-like activity in total homogenate.

224 The protease showed a trypsin-like activity with optimal temperature of 40 deg

225 is "locked" into the catalytically competent trypsin-like configuration upon benzamidine binding as i

226 Cockroach, like other allergens, contains trypsin-like enzyme activity that contributes to allerge

227 8.89 and 92.88% of nitric oxide synthase and TRYPSIN-like enzyme sites in Catalytic Site Atlas, and s

228 Prekallikrein (PK) is the precursor of the trypsin-like plasma protease kallikrein (PKa), which cle

229 is the formation of the complex between the trypsin-like protease coagulation factor VIIa (FVIIa) an

230 This function of FAM111A requires an intact trypsin-like protease domain, the PCNA interaction, and

231 the visceral mass of M. charruana contains a trypsin-like protease that can generate peptides from ca

232 ated by cleavage of fibrinogen and PAR1, the trypsin-like protease thrombin activates the anticoagula

233 Activated protein C is a trypsin-like protease with anticoagulant and cytoprotect

234 ing in the complex seven-member human airway trypsin-like protease/differentially expressed in a squa

235 The conformational properties of trypsin-like proteases and their zymogen forms remain co

236 ypothesis that the putative zymogens of many trypsin-like proteases are actually active proteases, ex

237 Activity in trypsin-like proteases is the result of proteolytic clea

238 ) viruses is cleaved at a monobasic motif by trypsin-like proteases.

239 ed to identify, isolate and characterize the trypsin-like proteinases in German cockroach allergen ex

240 he specificity binding pocket of trypsin and trypsin-like proteins from Serine-protease dataset.

241 triptase-2 (MT2) is a type-II transmembrane, trypsin-like serine protease that is predominantly expre

242 Factor D is a trypsin-like serine protease with a narrow specificity f

243 Human beta-tryptase, a tetrameric trypsin-like serine protease, is an important mediator o

244 The trypsin-like serine protease, urokinase-type plasminogen

245 Although trypsin-like serine proteases have flexible surface-expo

246 ms and conformational flexibility of uPA and trypsin-like serine proteases in general.

247 in advanced stage tumors and is activated by trypsin-like serine proteases that are highly expressed

248 Proteasome chymotrypsin-like, trypsin-like, and caspase-like activities were increased

249 Using the trypsin-like, clotting protease thrombin as a relevant m

250 aration steps were omitted by implementing a trypsin/Lys-C enzyme-immobilized multichannel reactor (M

251 Although trypsin-mediated cell death leads to pancreatic injury i

252 ed to trypsin either through autoactivation (trypsin-mediated trypsinogen activation) or by the lysos

253 sinopril and fosinoprilat) and two peptides (trypsin-modulating oostatic factor/TMOF and a bradykinin

254 Finding the conditions for stabilization of trypsin offers novel opportunities for the application o

255 chymotrypsin can act better and faster than trypsin on peptide bonds of proteins.

256 in at 20 degrees C) prior to hydrolysis with trypsin only and trypsin-pronase.

257 hysical properties, legumain (as compared to trypsin or GluC) enabled complementary proteome and prot

258 olysis of the extracted proteins with either trypsin or HT generated more antioxidant activity than h

259 trypsin fails to restore specificity against trypsin or human neutrophil elastase.

260 owing steps: PNGaseF treatment combined with trypsin or pepsin digestion was used to determine the gl

261 when removed from cells, resist digestion by trypsin or pepsin, and survive boiling in sodium dodecyl

262 elta ligand was eliminated by treatment with trypsin or removal of glycosaminoglycans, and also suppr

263 By using chymotrypsin, trypsin, or trypsin followed by chymotrypsin to digest d

264 dorsal root ganglia neurons with CS, NE, or trypsin, PAR(2) responsiveness initially declined, consi

265 5 and 6, and were subjected to hydrolysis by trypsin, pepsin and a bacterial protease called HT for 1

266 Trypsin predigestion also improved the protein identific

267 Trypsin-PRM leads to sensitive and highly correlated (R(

268 ept, clinical serum samples were examined by trypsin-PRM, and a slightly higher adduction level was o

269 C) prior to hydrolysis with trypsin only and trypsin-pronase.

270 ole in clearance of pathologically activated trypsin protecting against pancreatic injury.

271 unds, the volatile (E)-alpha-bergamotene and trypsin proteinase inhibitors (TPIs), which are also fou

272 tin-like material generated was resistant to trypsin proteolysis and displayed mechanical properties

273 g RNA against EP2, or an agonist of F2R like trypsin receptor 1 (F2RL1, also called protease-activate

274 idic design to eliminate the requirement for trypsin release of cells to suspension, prior to single-

275 contrast, B3+4(V) capsids were unstable with trypsin, releasing several peptides in addition to the p

276 While trypsin remains the preferred enzyme for quantification

277 e highly reactive oxygen species (hROS)- and trypsin-responsive 11-mercaptoundecanoic acid-modified g

278 ) Emulsion (MADE) method, we fabricated both trypsin-responsive and neutrophil elastase-responsive po

279 formation with the inhibitor Lys(32) in the trypsin S1 pocket, the inhibitor C-terminal residue Ser(

280 loroquine, histamine, lysophosphatidic acid, trypsin, SLIGRL, beta-alanine, BAM8-22), and scratching

281 Using a trypsin-specific activity-based probe, we detected three

282 e beneficial use of enzymes complementary to trypsin, such as Glu-C, Asp-N, Lys-N, Arg-C, LysargiNase

283 in vitro gastrointestinal digestion (pepsin-trypsin system) greatly improved the antioxidant propert

284 GFPc that functions as both a surfactant and trypsin target.

285 ene-1-carboximidamide binding to the protein trypsin, the exchange term is found to be equal within e

286 The use of trypsin, the gold standard protease in proteomics, as th

287 In this study trypsin (TIA) and chymotrypsin inhibitory (CIA) activiti

288 t popular bottom-up proteomics workflow uses trypsin to enzymatically cleave proteins C-terminal to l

289 ns) was observed for the pores formed by the trypsin-treated toxin.

290 orms of the toxin (native, midgut juice- and trypsin-treated), with conductances ranging from 28 to 6

291 The cell detachment ratio by trypsin treatment was slightly higher than that induced

292 Moreover, in an in vitro digestive pepsin-trypsin treatment, 30% of quinoin is resistant to enzyma

293 loop of a CRN that produces oscillations of trypsin under out-of-equilibrium conditions.

294 The WPC 35 hydrolysis with trypsin was carried out for 4.31 h at 41.1 degrees C wit

295 Trypsin was leached off under high ionic strength (3M Na

296 resulting from a subsequent digestion using trypsin were analyzed by reverse-phase liquid chromatogr

297 pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food pres

298 Trypsin, which cuts specifically after the basic residue

299 proteome and sequence coverage achieved with trypsin, with unique possibilities for the characterizat

300 The digestion of ubiquitinated proteins with trypsin yields a glycine-glycine remnant bound to the mo