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1 tect sequences that are not accessible after tryptic digest.
2 opeptides can be isolated selectively from a tryptic digest.
3 entative of the range in peptides found in a tryptic digest.
4 eptides detected from a Shewanellaoneidensis tryptic digest.
5 proximately 700 peptides for a S. oneidensis tryptic digest.
6 ndem mass spectrometry (MS/MS) analysis of a tryptic digest.
7 with both peptide standards and a HeLa cell tryptic digest.
8 ified as serine 461 by HPLC-MS analysis of a tryptic digest.
9 s of angiotensin peptides and a cytochrome c tryptic digest.
10 liquid chromatography-mass spectrometry of a tryptic digest.
11 mino acid sequence by Edman degradation of a tryptic digest.
12 f 6%, from a 62.5 ng load of human cell line tryptic digest.
13 mplarily shown for a pesticide mixture and a tryptic digest.
14 ted form, prepared in a bovine serum albumin tryptic digest.
15 ve to abundant nonphosphorylated peptides in tryptic digests.
16 cities of 130-420 for analytes from proteome tryptic digests.
17 ealed by the appearance of a new fragment in tryptic digests.
18 nalysis of both soluble and membrane protein tryptic digests.
19 by SDS-PAGE and LC-MS/MS analysis of in-gel tryptic digests.
20 pplied for the study of whole-proteome mouse tryptic digests.
21 for CE/MS of peptides, proteins, and protein tryptic digests.
22 -MS) for the high-resolution analysis of mAb tryptic digests.
23 Dissociation (HCD) spectra or spectra of non-tryptic digests.
24 s conducted with peptide solutions mimicking tryptic digests.
25 verified using standard peptides and protein tryptic digests.
26 romatography/tandem mass spectrometry of NFT tryptic digests.
27 tandem mass spectrometry (MS/MS) analysis of tryptic digests.
28 dem mass spectrometry (LC-MS/MS) analyses of tryptic digests.
29 luding neuronal tissue releasate and protein tryptic digests.
33 or measurements of a complex fungal proteome tryptic digest and provide improved confidence or number
34 ilico identify the peptides from the E. coli tryptic digest and show the increased confidence in pept
35 c acid improved the signal-to-noise ratio of tryptic digests and gave a 3-fold increase in the number
37 cations were studied by LC-MS/MS analyses of tryptic digests and included DTT-reversible events, e.g.
38 approach to identification of peptides (from tryptic digests) and to separation of charge-state distr
39 was evaluated using a Shewanella oneidensis tryptic digest, and approximately 15-amol detection limi
40 ior to cleanup by immunoaffinity extraction, tryptic digest, and preconcentration by solid-phase extr
41 The photolabeled kinase was subjected to tryptic digest, and the fragments were separated by chro
42 f fluorescein-labeled model peptides and BSA tryptic digest are demonstrated using the microchip HPLC
43 n, some phosphorylated peptides generated by tryptic digest are small and hydrophilic and, thus, are
46 pray ionization mass spectrometry mapping of tryptic digests before and after liver transplantation.
47 mics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass
48 ade in terms of the capability of separating tryptic digests between the mixed-mode phase and C18 rev
49 quantities (100 ng) of a Pyrococcus furiosus tryptic digest, but with mass-limited amounts (5 ng) CE
52 is by MALDI-FT-ICR-MS, de novo sequencing of tryptic digested CHH by nano-LC/ESI-Q-TOF MS and intact
53 ntiated from nonmodified peptides in complex tryptic digests created upon proteolysis of proteins aft
54 racterization of products from these partial tryptic digests demonstrated that approximately 90% of t
55 ional mapping of phosphopeptides in complete tryptic digests demonstrated that the reduced phosphoryl
57 online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achi
60 lack widow spiders and performed in-solution tryptic digests followed by MS/MS analysis to identify n
61 were determined by mass spectral analyses of tryptic digest fragments of cross-linked hemoglobin, emp
63 tified by mass spectrometric sequencing of a tryptic digest from the protein band on SDS-PAGE associa
64 is further demonstrated for the analysis of tryptic digests from different colorectal cancer cell li
65 The LC-MS chromatographic peak profiles of tryptic digests from OXY, bovine Hb, human Hb, and equin
66 erine-containing peptide was identified from tryptic digests from Sulfolobus solfataricus P1 by liqui
69 roups were identified from just 1 ng of HeLa tryptic digest hereby increasing detection sensitivity a
70 e separation of nucleic acids, proteins, and tryptic digests in combination with UV absorbance detect
71 Peptide sequences identified in the 70 kDa tryptic digest include iPLA(2)beta residues 7-53, sugges
72 ixtures of BSA and human serum albumin (HSA) tryptic digests indicated that ion series searches with
75 ing a 10-1000 fold-excess of enzyme, (v) the tryptic digest is directly transferred to a perfusion di
76 ngly and multiply phosphorylated peptides in tryptic digests is demonstrated at low-nanomolar protein
79 tides as well as biological peptides bearing tryptic digest-like features and peptides with post-tran
80 it-lamp stereophotomicroscopy, western blot, tryptic-digest/mass spectrometry electrospray ionisation
81 s spectrometry (MS/MS) analysis of an in-gel tryptic digest matched the protein sequence of thimet ol
83 e containing 20 fmol of human growth hormone tryptic digest mixed with the bovine serum protein diges
84 ximately 400 for the separation of a complex tryptic digest mixture when the sample preparation inclu
88 okinetic elution and sample stacking using a tryptic digest of 16 proteins to maximize peptide identi
89 ed peptides were purified from an exhaustive tryptic digest of [32P]ANPP-labeled Na+/K(+)-ATPase.
90 ilar results were obtained when evaluating a tryptic digest of a cellular lysate, representing a more
91 ure phosphoserine-containing peptides from a tryptic digest of a complex peptide mixture in which its
93 strated by fractionating the separation of a tryptic digest of a known protein mixture onto the micro
94 r isoelectric focusing (IEF) of a methylated tryptic digest of a mixture of alpha-S-casein and beta-c
95 ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared
96 electrospray interface for the analysis of a tryptic digest of a sample of intermediate protein compl
104 a carboxyl-terminal fragments from a partial tryptic digest of beta while DAP-Q labels only the carbo
108 yses of complex biological samples such as a tryptic digest of bovine serum albumin and a carnitine s
109 peptides from injection of only 1 pmol of a tryptic digest of bovine serum albumin using an eluent f
110 illary LC for the analysis of substance P, a tryptic digest of bovine serum albumin, and a phosphopep
112 t was added as a trace marker component to a tryptic digest of bovine serum proteins or to a human gr
116 Peptide sequences of four peptides from a tryptic digest of cytochrome c (approximately 1 pmol dep
117 The approach is illustrated by examining a tryptic digest of cytochrome c and by identifying a pept
119 bilities of 2D FT-ICR MS are explored with a tryptic digest of cytochrome c with both ECD and IRMPD a
122 ber of peptides and proteins identified in a tryptic digest of E. coli cell lysate increased by 13% a
123 the enzyme transglutaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated form
124 e peptide-specific hybridomas responded to a tryptic digest of HEL or to naturally processed HEL pept
125 ously uncharacterized phosphopeptides from a tryptic digest of human beta 4 integrin, isolated from c
126 igh performance liquid chromatography of the tryptic digest of inactivated enzyme yields a single rad
128 wing fast protein liquid chromatography of a tryptic digest of MAP2, suggesting that multiple modific
129 o acid sequence coverage was attained from a tryptic digest of myoglobin in < 5 min from an 80% aceto
132 ctural analysis of the peptides of the total tryptic digest of oat phyA, we found that the photorecep
133 system was used to identify a peptide from a tryptic digest of ovalbumin using standard addition and
139 selective enrichment of phosphopeptides from tryptic digest of standard protein (alpha-casein, beta-c
145 pparatus for complex mixtures, 5 microg of a tryptic digest of the cytosolic proteins of yeast was an
146 mance liquid chromatographic analysis of the tryptic digest of the HOCl-treated proteinase demonstrat
147 On the basis of complete mapping of total tryptic digest of the iodoacetamide-modified oat phytoch
149 -glycosylated peptide from an unfractionated tryptic digest of the lectin of the coral tree, Erythrin
151 strated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.
152 quencing of peptides obtained from an in-gel tryptic digest of the monomer and tetramer by tandem mas
154 liquid chromatography/ESIMS analysis of the tryptic digest of the protein followed by subsequent mat
156 demonstrated by applying 50 and 38 fmol of a tryptic digest of the proteins beta-lactoglobulin and bo
161 eparations of a model mixture of peptides, a tryptic digest of trypsinogen, and < 0.05% of an individ
162 version in TPI, which could be detected in a tryptic digest of tumor-derived TPI by mass spectrometry
164 ll of reversed-phase C18 functionality) to a tryptic digest of whole Jurkat cell lysate to estimate t
166 omparison of two-dimensional peptide maps of tryptic digests of 32P-labeled recombinant cPLA2 and hum
167 by high performance liquid chromatography of tryptic digests of 32P-labeled recombinant cPLA2 showed
176 I)-IMAC and ZrO2, was also carried out using tryptic digests of both simple and moderately complex pr
177 the abundances of phosphorylated peptides in tryptic digests of bovine beta-casein and protein kinase
180 s pump to perform nanoflow HPLC separations; tryptic digests of bovine serum albumin (BSA), transferr
181 e signature 2-oxo-acid N-terminal peptide in tryptic digests of bronchoalveolar lavage fluid from pat
182 major phosphorylation peaks were detected in tryptic digests of cdb3 separated by reverse phase HPLC.
188 electivity of the approach are presented for tryptic digests of FP-biotinylated trypsin and FP-biotin
189 annosylated peptides from complex samples of tryptic digests of HEK293 and MCF10A whole cell extracts
190 s spectrometry, a peptide (obtained from the tryptic digests of HOCl-treated cyt c) corresponding to
191 sites are enriched by the modified tips from tryptic digests of horse radish peroxidase, chicken avid
192 ionization mass spectrometry (ESI-LC/MS) of tryptic digests of human alphaB-crystallin in the presen
197 ies or post-translational modifications from tryptic digests of individual proteins as well as whole
198 n sites after ablation of CypD, we subjected tryptic digests of isolated cardiac mitochondria from wi
200 e phosphorylation sites were detected in the tryptic digests of middle and C-terminal regions of IRS-
201 Alpha-ZrPN were applied for the analysis of tryptic digests of mouse liver and leukemia cell phospho
204 MALDI-TOF mass spectrometric analyses of tryptic digests of platelet-derived FV peptides detected
206 s was demonstrated with several peptides and tryptic digests of protein mixtures by LC-MS/MS experime
220 f flight mass spectrometry was used to study tryptic digests of SHV-1 and S130Gbeta-lactamases (+/- i
225 ity and selectivity is demonstrated with the tryptic digests of the naturally phosphorylated proteins
226 et-Tyr-Trp cross-link (as probed by LC/MS on tryptic digests of the protein), exhibited any correlati
227 S) cross-linked precursors, derived from the tryptic digests of three model proteins (Human Serum Alb
229 echniques was applied to the analysis of the tryptic digests of three well-characterized protein mixt
230 tted the identification of 111 proteins from tryptic digests of total hair from AKR/J-hid/hid mice, w
231 k areas of the two transition fragments from tryptic digests of whey proteins in stored milk protein
232 deglycosylated and intact glycopeptides from tryptic digests of whole influenza virus, we determined
233 furosine results indicated that MRM based on tryptic digests of whole products was a feasible method
235 r the gradient separation of peptides from a tryptic digest on a 27-cm-long capillary packed with 1.0
236 B was fully characterized using 100 fmol of tryptic digest on a three-dimensional ion trap mass spec
238 xtended now for the direct derivatization of tryptic digests originating from 1-5 microg of proteins
242 to measure collision cross sections for 968 tryptic digest peptide ions obtained from digestion of c
245 was illustrated by analysis of a mixture of tryptic digest peptides using high- and low-resolution i
247 tified and quantified the abundance of 1,056 tryptic-digested peptides, representing 163 proteins in
249 muFFE analysis of a Chromeo P503-labeled BSA tryptic digest produced a 2D separation that made effect
250 mensional separation of bovine serum albumin tryptic digest produced a peak capacity of 4200 (110 in
251 rified using a set of commercially available tryptic digest protein standards analyzed using an ABI 4
254 o provide a simple extension of the existing tryptic digest protocols to include carbohydrate analysi
256 dataset, and up to 77% more spectra from non-tryptic digests, relative to a fully supervised approach
257 ing these recommendations on 0.2 ng/muL HeLa tryptic digest results in a 10-fold increase in terms of
259 ization-mass spectrometry analyses of SERCA1 tryptic digests revealed ca. 66% coverage of the protein
261 MS/IMS/TOF performance for a protein mixture tryptic digest reveals high orthogonality between FAIMS
262 proteins were identified from a 50 ng in-gel tryptic digest sample combining five cuts in a single LC
264 Shewanella oneidensis proteins from a 300-ng tryptic digest sample in a single 4-h LC-MS/MS analysis.
265 f the platform was evaluated using an in-gel tryptic digest sample of a gel fraction (15-40 kDa) of a
267 en demonstrated using a bovine serum albumin tryptic digest separated by capillary LC where multiple
268 l 60 F(254S) plate and peptides from protein tryptic digests separated on a ProteoChrom HPTLC Silica
270 Isolation of the labeled peptide from the tryptic digest shows that Tyr(79) is the only enzymic am
271 The strategy includes periodate oxidation of tryptic digests, solid-phase enrichment of glycopeptides
272 omole amounts of crudely purified samples of tryptic digest solutions of horse cytochrome c and bovin
273 f small molecules and a bovine serum albumin tryptic digest, TASF improved the peak shape and resolut
275 e photolabeled PKCdelta C1B was subjected to tryptic digest, the fragments were separated by online c
278 anoLC-FAIMS-MS/MS of an unfractionated yeast tryptic digest using the modified FAIMS device identifie
279 xoid has been established by analysis of its tryptic digest using two-dimensional liquid chromatograp
283 glycosylated via an MS/MS experiment, so the tryptic digest was deglycosylated to confirm the presenc
284 e peptide 66LVNEVTEFAK75, also formed in the tryptic digest, was used as the native reference peptide
286 tein groups from as little as 250 pg of HeLa tryptic digest, we demonstrate outstanding sensitivity w
287 eline resolved, and peptides from an albumin tryptic digest were much better resolved than with exist
288 h-efficiency, two-dimensional separations of tryptic digests were achieved using glass microfluidic d
290 9 and 12 amino acids that represent typical tryptic digests were designed, synthesized, and analyzed
291 ides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly imp
295 with trypsin in protonated solution, and the tryptic digests were then analyzed via liquid chromatogr
296 m fetuin, glycophorin A, ovalbumin and gp120 tryptic digests were used to build a spectral database o
297 t of casein phosphopeptides from a simulated tryptic digest with bovine serum albumin (BSA:casein, 10
298 ocol is based on glycopeptide selection from tryptic digests with serial lectin affinity chromatograp
299 Proteomic experiments performed on HeLa tryptic digests with the modified mass spectrometer impr
300 e liquid chromatography (HPLC) analysis of a tryptic digest yielded an octapeptide within the insert