ライフサイエンスコーパス: tryptic digestion

1 ion of intact protein ions (i.e. without any tryptic digestion).

2 nd CTP protect a 67 kDa fragment of Lon from tryptic digestion.

3 gel electrophoresis and subjected to in-gel tryptic digestion.

4 digestion or treated directly by in-solution tryptic digestion.

5 hat can be robustly detected following rapid tryptic digestion.

6 ydrodynamic radius measurements, and limited tryptic digestion.

7 t mass spectrometry (MALDI-TOF MS) following tryptic digestion.

8 ponding to p125 was excised and subjected to tryptic digestion.

9 borating this, tRNA(Glu) protected MnmA from tryptic digestion.

10 ated by the resistance of the heterodimer to tryptic digestion.

11 nd Anchym2 zymogens are activated by partial tryptic digestion.

12 ix individual spots were subjected to in-gel tryptic digestion.

13 in this single structural domain protein by tryptic digestion.

14 aining the expected shortened fragment after tryptic digestion.

15 with the anticipated results of a simulated tryptic digestion.

16 uction products, which were stable following tryptic digestion.

17 n, cysteine alkylation, buffer exchange, and tryptic digestion.

18 e by more than 100-fold compared with direct tryptic digestion.

19 peptides derived from beta-lactoglobulin in tryptic digestion.

20 turn structure and the stereoselectivity of tryptic digestion.

21 BSA peptides were generated by tryptic digestion.

22 njunction with endoglycosidase treatment and tryptic digestion.

23 in comparison to the commonly used one-step tryptic digestion.

24 als from MTs and renders them susceptible to tryptic digestion.

25 of the complex peptide mixtures afforded by tryptic digestion.

26 posed of neutral phospholipids, protect from tryptic digestion a small portion of the segment that co

27 cular species derived from trastuzumab after tryptic digestion: a stable signature peptide (FTISADTSK

28 ylamide gel electrophoresis and subjected to tryptic digestion and amino acid sequence analysis.

29 s extracted from gel slices and subjected to tryptic digestion and analysis by mass spectrometry.

30 erwise, the reverse reaction occurred during tryptic digestion and analysis.

31 onto a polymer surface, followed by in situ tryptic digestion and comparative analysis using DESI-MS

32 such as Cdc42 and histone II-AS, followed by tryptic digestion and comparative two-dimensional phosph

33 Here we describe simultaneous tryptic digestion and gradual unfolding of native protei

34 by means of (immuno-)affinity purification, tryptic digestion and LC-HRMS/MS was developed.

35 Tryptic digestion and liquid chromatography electrospray

36 in serum using affinity techniques prior to tryptic digestion and liquid chromatography-tandem mass

37 Subsequent tryptic digestion and liquid chromatography/tandem mass

38 apped protein adducts were then subjected to tryptic digestion and MS analyses.

39 on blocks lysine side chains, and subsequent tryptic digestion and N-terminal peptide derivatization

40 acetamide (IE-TFA) followed by an exhaustive tryptic digestion and on-line HPLC-UV-electrospray MS an

41 ]diisopropylfluorophosphate and subjected to tryptic digestion and partial amino acid sequencing.

42 cal cross-linking of the complex followed by tryptic digestion and peptide identification through mat

43 m the acetylation of Ser516 was confirmed by tryptic digestion and peptide mapping of COX-2 labeled w

44 Tryptic digestion and peptide mapping of COX-2 reacted w

45 rotein subunit was purified and subjected to tryptic digestion and peptide sequence analyses.

46 d according to a shotgun protocol, and after tryptic digestion and prefractionation using strong cati

47 e site at Asn 317 was obtained after limited tryptic digestion and RP-HPLC.

48 Tryptic digestion and subsequent protein sequencing of t

49 crosslinking sites were identified following tryptic digestion and tandem mass spectrometry analysis.

50 arching, selected proteins were subjected to tryptic digestion and the resulting mixtures were analyz

51 mal membranes, and are readily removed after tryptic digestion and therefore may be membrane inserted

52 autophosphorylation determined after partial tryptic digestion and two-dimensional peptide analysis w

53 ethods, including total amino acid analysis, tryptic digestion, and collision-induced dissociation-el

54 ltistep sample cleanup at the protein level, tryptic digestion, and isotope dilution mass spectrometr

55 atography of protein on cobalt-loaded beads, tryptic digestion, and MALDI MS analysis are performed i

56 ragments were isolated from cell surfaces by tryptic digestion, and partial amino-terminal amino acid

57 Biomarker peptides were selected, after tryptic digestion, and quantified by multitarget nanoHPL

58 al approach via immunoaffinity purification, tryptic digestion, and subsequent detection by HPLC-HRMS

59 s include isolation of cross-linked species, tryptic digestion, and subsequent peptide sequencing.

60 n reactions, poly(C) binding assays, limited tryptic digestions, and the bicyclomycin inhibition kine

61 Using protection against tryptic digestion as a measure of the activated conforma

62 uction, and (iv) increased susceptibility to tryptic digestion as a result of conversion of negativel

63 DNEQ and delta-DSE actins protected S1 from tryptic digestion as well as the wild type and rabbit ac

64 use of a conformation-specific antibody and tryptic digestion assays, we showed that glycosylation a

65 f this method for sequencing peptides from a tryptic digestion at subpicomole levels and for identify

66 In contrast, tryptic digestions at Lys-61 and -68 occurred at the sam

67 only used in protein solubilization prior to tryptic digestion, but the presence of the DS(-) hampers

68 , ubiquitinated peptides were enriched after tryptic digestion by peptide immunoprecipitation using a

69 ioxygen-saturated buffer was investigated by tryptic digestion coupled mass spectrometry.

70 Upon tryptic digestion, cross-linked peptides were identified

71 Collectively, our kinetic as well as tryptic digestion data suggest that both nucleotide bind

72 The sensitivity of p72 to tryptic digestion differed considerably in the presence

73 In this work, tryptic digestion experiments were performed to detect t

74 Finally, tryptic digestion followed by analysis using MALDI TOF m

75 p- or down-regulated spots were subjected to tryptic digestion followed by identification using matri

76 Global tryptic digestion followed by liquid chromatographic/mas

77 We performed GeLC-MS/MS (in-gel tryptic digestion followed by liquid chromatography-tand

78 In-gel tryptic digestion followed by mass spectral fingerprinti

79 Using tryptic digestion followed by mass spectrometry and amin

80 ein accurate mass measurement, the other for tryptic digestion followed by MS and MS/MS analyses.

81 In-gel tryptic digestion followed by nano-liquid chromatography

82 nocysteine (U, SeCys), were identified after tryptic digestion followed by their separation.

83 Tryptic digestion, followed by MS, confirmed the presenc

84 This method involves optimization of in situ tryptic digestion, followed by reproducible and uniform

85 generated comparable or better results than tryptic digestion for protein identification.

86 erformance liquid chromatography to on-probe tryptic digestion for subsequent analyses by MALDI-TOF M

87 Using preparative SDS-PAGE, in-gel tryptic digestion, high pressure liquid chromatography,

88 Our new procedure involves tryptic digestion in aqueous solution buffered to pH 8-8

89 ing protein in 18O water (H218O) followed by tryptic digestion in regular water (H216O).

90 Tryptic digestion in the presence of DDOPM was more effi

91 paraffin-embedded samples enhances on-tissue tryptic digestion, increasing peptide detection using ma

92 Limited tryptic digestion indicated that the central region of a

93 as enhanced after reduction, alkylation, and tryptic digestion, indicating that the three-dimensional

94 however, R172W protein was more sensitive to tryptic digestion, indicative of a change in protein con

95 Our results demonstrate that LFASP-based tryptic digestion is efficient, robust, reproducible, an

96 Tryptic digestion is followed by strong cation exchange

97 pL sample volume required for this on-column tryptic digestion is, to our knowledge, the smallest yet

98 After delipidation and tryptic digestion, mass spectrometry was used to identif

99 radigm of chemical cross-linking followed by tryptic digestion, mass spectrometry, and database searc

100 A special tryptic digestion method has been developed to facilitat

101 was identified, by using LC-MS/MS, from the tryptic digestion mixture of the reaction products of th

102 rometer has been applied for the analysis of tryptic digestion mixtures of the protein bovine serum a

103 Moreover, the tryptic digestion of a complex protein mixture in an org

104 The method used was based on tryptic digestion of a protein followed by quantificatio

105 sity coating, while the SPME protocol on the tryptic digestion of a protein supported that enzymes we

106 ect on DNase I binding and the fast phase of tryptic digestion of actin.

107 e mixtures, including one generated from the tryptic digestion of alpha-casein.

108 In silico tryptic digestion of amaranth globulins was carried out

109 esalting steps, and accomplishes the reduced tryptic digestion of an IgG2 mAb in a mildly acidic cond

110 In an LC/MS/MS analysis of a tryptic digestion of an IgG2 monoclonal antibody, 1712 p

111 isulfide bond C(66)-C(160) by LC-MS/MS after tryptic digestion of BLG.

112 First, limited tryptic digestion of CKI epsilon produces a protease-res

113 Tryptic digestion of differentially labeled carbonic anh

114 n subfragment-1 (S1) ATPase activity nor the tryptic digestion of G-actin and F-actin at the Lys-61 a

115 Tryptic digestion of hCTR1 occurred within the cytoplasm

116 Tryptic digestion of HGXPRTase labeled with iodo[2-14C]a

117 dies of the peptide fragments resulting from tryptic digestion of KatG(M255I) confirmed the presence

118 Tryptic digestion of loop 1 slightly accelerated the rel

119 tion of mAbs with intact disulfide bonds and tryptic digestion of mAbs after reduction and alkylation

120 e proteome that relies on solubilization and tryptic digestion of membrane proteins in a buffer conta

121 followed by a single-tube solubilization and tryptic digestion of membrane proteins.

122 Tryptic digestion of MyBP-H yielded two peptides: approx

123 The workflow comprises a 2 h tryptic digestion of PAPs in suspension, an immunoaffini

124 esent study describes a workflow for in situ tryptic digestion of plant seed tissue for MALDI MS imag

125 nd sequencing of phosphopeptides obtained by tryptic digestion of protein extracts from HeLa cells.

126 to dissociate ADA-drug bindings, followed by tryptic digestion of protein pellets and subsequent LC-M

127 of small molecules and peptides obtained by tryptic digestion of proteins and entire proteins.

128 em can realize rapid, efficient and reusable tryptic digestion of proteins by taking advantage of its

129 Improved in-solution tryptic digestion of proteins in terms of speed and pept

130 microsequencing of oligopeptides obtained by tryptic digestion of purified IscS was used to design a

131 buffered methanol-based solubilization, and tryptic digestion of purified plasma membrane is describ

132 tion of the peptide fragments resulting from tryptic digestion of recombinant Mycobacterium tuberculo

133 technique for protein separation, and in-gel tryptic digestion of resolved protein bands has enhanced

134 sidues (the longest peptide identified after tryptic digestion of S. oneidensis) with an average accu

135 This prediction was verified by tryptic digestion of SERT-expressing membranes: in the a

136 the determination of thiolic peptides after tryptic digestion of serum albumins from different speci

137 domain previously observed following limited tryptic digestion of spectrin protein.

138 The sequence of a peptide obtained after tryptic digestion of the 42-kDa protein allowed the spec

139 Analysis of labeled peptides generated by tryptic digestion of the affinity-labeled proteins ident

140 After in-solution tryptic digestion of the aqueous protein material extrac

141 alyzing conserved peptides, derived from the tryptic digestion of the B subunits.

142 ido-[alpha-32P]ATP into the 2-5A synthetase, tryptic digestion of the covalently 32P-labeled enzyme,

143 Tryptic digestion of the CYP3A4-[(3)H]ritonavir incubati

144 chromatography (IMAC), which was followed by tryptic digestion of the enriched sample and quantificat

145 itive FAB mass spectrometry after exhaustive tryptic digestion of the Er-ITC-modified Ca-ATPase.

146 Tryptic digestion of the H, L, and P polypeptide chains

147 Tryptic digestion of the heme-protein adduct and Edman s

148 e near the phosphorylation site and favoring tryptic digestion of the Lys536-Asn537 peptide bond.

149 ed as the position of the reactive ligand by tryptic digestion of the modified protein and by mutatio

150 Tryptic digestion of the protein yielded peptides that i

151 inding sites on RYR1 are destroyed by a mild tryptic digestion of the sarcoplasmic reticulum membrane

152 acid sequences from two peptides, derived by tryptic digestion of the structural subunit of one type

153 re, we report on the study of the effects of tryptic digestion of these loops on the motor and enzyma

154 Tryptic digestion of these oxidized proteins followed by

155 eparations for complex peptide mixtures from tryptic digestion of yeast cytosol fractions were obtain

156 n in a high boiling point solvent, and (iii) tryptic digestions of cytochrome c at 22 and 40 degrees

157 Tryptic digestions of myosin, which produced heavy merom

158 ermore, after the reduction, alkylation, and tryptic digestion, only 4 of a possible 25 asparagine re

159 h an orthogonal traceable method using total tryptic digestion, peptide separation, and isotope dilut

160 Carbamylation of proteins followed by tryptic digestion produced peptides similar to those exp

161 duced amino acid sequence with a celery M6PR tryptic digestion product.

162 Analysis of the tryptic digestion products by immunoblotting, N-terminal

163 ed by MALDI-TOF mass spectrometry, and their tryptic digestion products sequenced via Shotgun proteom

164 Tryptic digestion products were sampled, detected, and i

165 odulin (either form), bound to RYR1 prior to tryptic digestion, protects both the apocalmodulin and C

166 ences in relative peptide concentrations for tryptic digestions ranging from 15 min to 48 h.

167 mization of the conditions of extraction and tryptic digestion, restructured meat and blank values (t

168 s were generated based on a previous limited tryptic digestion result and hydrogen-deuterium exchange

169 eins, and reduced analytical variability for tryptic digestion, resulting in markedly faster and more

170 otocol used in route A involved the steps of tryptic digestion, reversed-phase chromatographic fracti

171 otocol, which includes cell lysis, overnight tryptic digestion, sample analysis and database searchin

172 hosphorylation using a combination of in gel tryptic digestion, SDS-PAGE and HPLC, MALDI-TOF MS analy

173 by the Fe3O4@Al2O3 MNPs followed by on-plate tryptic digestion, selective enrichment, and MALDI-MS an

174 sis of this product by mass spectrometry and tryptic digestion showed that it was a cyclic form of tr

175 lly toxic epitopes released after peptic and tryptic digestion, showing inefficiency as a treatment t

176 n can be achieved using this newly developed tryptic digestion station.

177 ication of 10 pg/mL, while introduction of a tryptic digestion step, followed by quantification of a

178 ents extracted from the pyriform gland after tryptic digestion support the assertion that PySp1 repre

179 Selective elution of captured protein, tryptic digestion, tandem mass spectrometry analysis, an

180 re used to show that after glycinylation and tryptic digestion, the mass spectrometric response from

181 After tryptic digestion, the peptides were analyzed using mass

182 After protein extraction and tryptic digestion, three to four selected marker peptide

183 We have used tryptic digestion to determine whether Ca(2+) can regula

184 After limited tryptic digestion to ensure the accessibility of only ex

185 the performance of "bottom-up" (in-solution tryptic digestion), "top-down" (intact protein fragmenta

186 We performed tryptic digestion using six different methods: in-gel, i

187 On-membrane tryptic digestion using this method requires half the ti

188 Tryptic digestion was performed in a small volume (3 mic

189 The tryptic digestion was physiologically significant, corre

190 12F protein by nucleotide as protection from tryptic digestion was unchanged.

191 The results of the capture after tryptic digestion were analyzed by MALDI TOF MS.

192 Oxidized peptides generated by tryptic digestion were identified by electrospray-Fourie

193 olyzing succinimide in H(2)(18)O followed by tryptic digestion were used to label and identify the si

194 sis of a LLDGSSTEIR glycopeptide released by tryptic digestion, which carried two variant structures,

195 tecting a characteristic peptide produced by tryptic digestion with an LOD of 3 pM.

196 ow volume protein analysis strategy based on tryptic digestion within the tip of a 1.5-microm capilla

197 ids reformation of disulfide bonds to enable tryptic digestion without alkylation of cysteine residue

198 mide treatment of PSP, followed by prolonged tryptic digestion yielded predominantly a 14C-labeled te