ライフサイエンスコーパス: two-photon

1 Employing in vivo two-photon (2-p) Calcium (Ca) imaging and 2-p targeted w

2 ate that such precision can be achieved with two-photon (2P) temporally focused computer-generated ho

3 large polythiophene dendrimers that serve as two-photon absorbers.

4 rescence imaging or data storage with common two-photon absorbing dyes, is dramatically improved.

5 erein proposed approach opens the way to use two-photon absorbing dyes, reaching the same performance

6 tion of 1(4+) is quantified and its use as a two-photon absorbing phototherapeutic is reported.

7 e we report a theoretical study of entangled two-photon absorption (ETPA) in organic chromophores, an

8 The pai-extended dyes show interesting two-photon absorption (TPA) properties, maintaining high

9 constitutes a unique material that exhibits two-photon absorption and near-infrared emission simulta

10 Their two-photon absorption bands reach into the NIR-II region

11 Observed two-photon absorption coefficients (beta ~75 cm/GW) and

12 -PDLLA form micelles that can be loaded with two-photon absorption compound (T1) and photosensitizer

13 In this study, the two-photon absorption cross section of 1(4+) is quantifi

14 he two co-crystals possess higher calculated two-photon absorption cross-sections than those of their

15 uously confirms the occurrence of the direct two-photon absorption in the dots.

16 ure nonlinear excitation intensity dependent two-photon absorption induced exciton photoluminescence

17 We conclude that the two-photon absorption induced PL is highly sensitive to

18 vely excited by the process of the entangled two-photon absorption is reported.

19 e optical probe, whilst maintaining the high two-photon absorption of over 500 GM.

20 As quantum dots, specifically the associated two-photon absorption of the wetting and barrier layers,

21 ctroscopy were used to identify a sequential two-photon absorption process, leading to excitation of

22 using near-infrared, NIR, and light through two-photon absorption, TPA.

23 behavior but led to the observation of high two-photon-absorption (TPA) cross sections (ca. 220 GM a

24 We here investigate two-photon-absorption (TPA)-induced photoluminescence of

25 tra, luminosity, fluorescence lifetimes, and two-photon absorptivity.

26 oth UV- and two-photon-mediated reactions; a two-photon action cross-section of 0.71-1.4 GM for the n

27 is current study, we have developed one- and two-photon-activated persulfide donors based on an o-nit

28 Two-photon activation of optogenetic actuators and calci

29 of these protected peptides by either UV or two-photon activation was used to initiate their subsequ

30 Two-photon adsorption, accompanied by anti-Stokes fluore

31 nts in GCaMP6s-expressing neurons for 920 nm two-photon and 1320 nm three-photon excitation.

32 Here, we addressed this question using two-photon and light-sheet calcium imaging in intact, be

33 CaImAn is suitable for two-photon and one-photon imaging, and also enables real

34 ) and L(4c), bearing two specific picolinate two-photon antennas (tailor-made for each targeted metal

35 Here, we demonstrate two-photon autofluorescence imaging of NAD(P)H and FAD t

36 plet) and vat polymerization-based (one- and two-photon) bioprinting.

37 Here, we have combined two-photon Ca(2+) imaging and single-cell electrophysiol

38 We utilized in vivo two-photon Ca(2+) imaging in awake mice expressing GCaMP

39 unction as determine by c-Fos expression and two-photon Ca(2+) imaging in awake mice.

40 Using in vivo two-photon Ca(2+) imaging of dLGN afferents in superfici

41 By employing in vivo two-photon Ca(2+) imaging of layer 2/3 cortical neurons

42 ination of in situ hybridization and ex vivo two-photon Ca(2+) imaging of the mouse spinal cord to es

43 Moreover, two-photon Ca(2+) imaging showed that presynaptic Na(+)

44 iation in the proximal apical dendrite using two-photon Ca(2+) photometry and focal TTX application.

45 activity of neurons of the lateral OFC using two-photon calcium imaging and investigated how OFC dyna

46 Projection-specific two-photon calcium imaging and optogenetic manipulations

47 Here we combine two-photon calcium imaging and optogenetics in tethered

48 By combining two-photon calcium imaging and patch-clamp electrophysio

49 an 'all-optical' combination of simultaneous two-photon calcium imaging and two-photon optogenetics,

50 Recently, several studies using two-photon calcium imaging and virtual navigation have i

51 e use fast, targeted, three-dimensional (3D) two-photon calcium imaging coupled with immunohistochemi

52 Two-photon calcium imaging during decision making reveal

53 ming large-scale physiological recording and two-photon calcium imaging in adult male and female mice

54 We performed in vivo two-photon calcium imaging in an experimental model of D

55 tivity of retinal axons using wide-field and two-photon calcium imaging in awake mouse thalamus acros

56 Using two-photon calcium imaging in CA1 while mice performed a

57 We used two-photon calcium imaging in female mice to characteriz

58 Using two-photon calcium imaging in identified cell types in a

59 Using two-photon calcium imaging in layers 2/3, we found that

60 We used fast in vivo two-photon calcium imaging in male mouse neocortex to re

61 Two-photon calcium imaging in mice has confirmed the pre

62 To address this, using in vivo two-photon calcium imaging in mice, we tracked the respo

63 We performed in vivo two-photon calcium imaging in neocortex during temperatu

64 method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and m

65 Two-photon calcium imaging of abducens neurons in contro

66 By performing two-photon calcium imaging of head-fixed male and female

67 use primary visual cortex (V1), we performed two-photon calcium imaging of layer 2/3 neurons and asse

68 Here we use two-photon calcium imaging of neural population dynamics

69 We used fast two-photon calcium imaging of neuronal populations (calc

70 Using in vivo two-photon calcium imaging of thalamocortical axons in m

71 tivity by combining in vivo 3D random-access two-photon calcium imaging of the dendritic spines of si

72 orly understood in vivo Here, we use in vivo two-photon calcium imaging of the vermal cerebellum in a

73 In vivo two-photon calcium imaging shows that DRG neuronal activ

74 Here, to address this gap, we used two-photon calcium imaging through an implanted lens to

75 rentiation of visual cortical areas, we used two-photon calcium imaging to characterize the effects o

76 We used stable GCaMP6f expression and two-photon calcium imaging to probe a very large spatial

77 Here, we used two-photon calcium imaging to record spontaneous activit

78 or studying spontaneous activity measured by two-photon calcium imaging using computational methods a

79 Finally, by combining two-photon calcium imaging with birth date labeling of g

80 We combined two-photon calcium imaging with genetic, pharmacological

81 Here we combined two-photon calcium imaging with whole-cell electrophysio

82 bination of slice electrophysiology, in vivo two-photon calcium imaging, and optical imaging of intri

83 Here, using in vivo two-photon calcium imaging, we find that PVT neurons pro

84 Using two-photon calcium imaging, we monitored the activity of

85 Using two-photon calcium imaging, we recorded hippocampal CA1

86 cell RNA sequencing and longitudinal in vivo two-photon calcium imaging, we surveyed functional alter

87 eously recording local network activity with two-photon calcium imaging.

88 novel head-fixed sucrose preference task and two-photon calcium imaging.

89 recording of OT neurons in awake mice using two-photon calcium imaging.

90 Using unsupervised clustering of two-photon calcium responses followed by inspection of a

91 Two-photon confocal and immunofluorescence microscopy wa

92 eratryl-based protecting groups and a useful two-photon cross-section.

93 n tissues of live mice, using bio-orthogonal two-photon cycloaddition and crosslinking of the polymer

94 tations from multiple labelers on nine mouse two-photon datasets.

95 Here we perform two-photon dendritic imaging with a genetically-encoded

96 line width, which we relate to the entangled two-photon density of states using a quantum electrodyna

97 design more exotic interactions, such as the two-photon Dicke model.

98 s at the B/Y avoided crossing and concurrent two-photon dissociation processes that involve the Y/Z a

99 as also compatible to near infrared PDT with two photon excitation (800 nm) for extensive bioapplicat

100 the release of biological effectors through two-photon excitation (2PE) in tissue culture and whole-

101 pedes the use of the technique in tissue, as two-photon excitation (2PE) is typically required to acc

102 Two-photon excitation (TPE)-based imaging overcomes the

103 absorption coefficients (beta ~75 cm/GW) and two-photon excitation cross-sections (eta(2)sigma(2) ~ 1

104 onversion by the PM-QD hybrid material under two-photon excitation is up to 4.3 times higher than the

105 The nonlinear nature of two-photon excitation may provide considerable advantage

106 Two-photon excitation microscopy (TPEM) has revolutioniz

107 Two-photon excitation microscopy with crisp-17 revealed

108 in vivo, thus allowing imaging by intravital two-photon excitation microscopy.

109 mbda1 = 850 and lambda2 = 110 nm for optimal two-photon excitation of blue and red chromophores.'.

110 also effectively activate M2 receptors using two-photon excitation with near-infrared light, which ov

111 the GaAs substrate and the wetting layer by two-photon excitation, which is accounted for by a model

112 for catalyst release, and responsiveness to two-photon excitation.

113 vation kinetics, and full responsivity under two-photon excitation.

114 rned illumination for photobleaching through two-photon excitation.

115 toluminescence of these dots by using direct two-photon excitation.

116 oherent anti-Stokes Raman scattering (CARS), two-photon excited autofluorescence (TPEF), and second-h

117 en (second harmonic generation) and elastin (two-photon excited autofluorescence), to obtain biochemi

118 Two-photon excited fluorescence (TPEF) microscopy is a l

119 ques, e.g., fluorescence lifetime imaging or two-photon excited fluorescence microscopy, to which Nil

120 Specifically, the one- and two-photon excited fluorescence of Nile Red turns out to

121 lasmon-enhanced optical sensor that utilizes two-photon excited luminescence of a planar gold film as

122 ramolecular approach is used to synthesize a two-photon excited near-infrared emissive co-crystalline

123 Two-photon excited near-infrared fluorescence materials

124 excitons show distinctly different one- and two-photon excited photoluminescence energies: from free

125 We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM)

126 FOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modifica

127 cipal component analysis to analyze pairs of two-photon fluorescence lifetime images of stratum basal

128 Here we present a technique using two-photon fluorescence lifetime imaging (2pFLIM) with n

129 Our analysis shows that two-photon fluorescence lifetime imaging permits the ide

130 t can be assessed using clinically available two-photon fluorescence lifetime microscopy systems.

131 taneous second harmonic generation (SHG) and two-photon fluorescence measurements on two different gr

132 Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid vol

133 porating a Bessel focus scanning module into two-photon fluorescence microendoscopy, we extended the

134 Using an ultrafast two-photon fluorescence microscope empowered by all-opti

135 r probes in brain tissue with one-photon and two-photon fluorescence microscopy and define the signal

136 scent probes, by comparison with correlative two-photon fluorescence microscopy of DNA and mitochondr

137 chnique using second-harmonic generation and two-photon fluorescence under single-laser excitation to

138 imension of the design strategy, a potential two-photon fluorescent substrate (NN) for CYP3A4 was eff

139 ll-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high

140 or the decay of the neutral pion pai(0) into two photons (gammagamma), leading to its unusually short

141 Here, we use two-photon GCaMP6f imaging to simultaneously monitor den

142 Here, using two-photon glutamate uncaging to induce plasticity at in

143 staining, reporter gene assays, RNA-Seq, and two-photon glutamate uncaging with calcium imaging, we s

144 and single-synapse currents, evaluated using two-photon glutamate uncaging.

145 Using two-photon holographic optogenetics in mouse primary vis

146 than the excitation level for the classical two-photon image.

147 Here, we performed time lapse in vivo two photon imaging in somatosensory cortex of adult mice

148 imultaneous electrophysiology and time-lapse two photon imaging to examine how spines change their st

149 axons that innervate BA (VTA(DA->BA)) using two-photon imaging and photometry in behaving mice.

150 andpass optical filters enables simultaneous two-photon imaging and visual stimulation over a large r

151 our knowledge, quantitative high-resolution two-photon imaging at 100 MPa of living microbes from al

152 din backbone can be optimized for intravital two-photon imaging by installing a red fluorophore.

153 Immunohistochemical studies and in vivo two-photon imaging confirmed that there is no significan

154 bipolar cell from the mouse retina based on two-photon imaging data.

155 In vivo two-photon imaging demonstrated that surveillance behavi

156 d, combinatorial spectral compatibility, and two-photon imaging depth.

157 Here, we use in vivo two-photon imaging during hyperspectral visual stimulati

158 d odorant sampling on MTC responses, we used two-photon imaging in anesthetized male and female mice

159 d by higher-resolution electrophysiology and two-photon imaging in awake macaques.

160 Using chronic two-photon imaging in awake mice, we observed spontaneou

161 Two-photon imaging in layer 2/3 of the primary somatosen

162 Two-photon imaging indicates that ADLumin-1 can efficien

163 In vivo two-photon imaging is a valuable technique for studies o

164 ing influenza virus-infected lung in vivo by two-photon imaging microscopy.

165 Here, using two-photon imaging of dendritic Ca(2+) signals, electric

166 We used time-lapse two-photon imaging of dendritic spine motility in acutel

167 We employ long-term, rapid light-sheet and two-photon imaging of early zebrafish retinogenesis to t

168 l imaging of head-fixed, behaving mice using two-photon imaging of fluorescent activity reporters has

169 We developed an optical technique based on two-photon imaging of fluorescently labeled extracellula

170 In vivo two-photon imaging of microglia in the intact brain has

171 We performed in vivo two-photon imaging of myelin sheaths along single axons

172 well as cellular and subcellular resolution two-photon imaging of neural structures up to 600 um dee

173 etically targeted single-unit recordings and two-photon imaging of Ntsr1-Cre+ L6 CT neurons in the pr

174 We addressed this question by using two-photon imaging of resting-state microvascular oxygen

175 We used large scale two-photon imaging of the nodose ganglia from our ex viv

176 Two-photon imaging revealed that these cues served as do

177 Two-photon imaging reveals enhanced hue-specific cell cl

178 n AD brains combined with repetitive in vivo two-photon imaging showed focal fibrinogen deposits asso

179 Transcranial two-photon imaging showed that deficits in extinction le

180 Time-lapse in vivo two-photon imaging showed that OVX-associated reduction

181 We developed a two-photon imaging technique that scans lines of excitat

182 ons for how to leverage fiber photometry and two-photon imaging to measure dLight1 transients in vivo

183 coded across the mouse visual system, we use two-photon imaging to measure receptive fields (RFs) and

184 Here, we use two-photon imaging to simultaneously measure maps of RF

185 demonstrate real-time movement-corrected 3D two-photon imaging with submicrometer precision.

186 Using fiber photometry recording and two-photon imaging, our ACh sensor also enabled sensitiv

187 Next, in vivo two-photon imaging, transcriptomics, and computational m

188 Using two-photon imaging, we detected evoked and spontaneous n

189 Using in vivo two-photon imaging, we show that locomotion-induced Ca(2

190 measured neural activity using widefield and two-photon imaging.

191 multiple cortical layers in awake mice using two-photon imaging.

192 pansion associated with LTP, as monitored by two-photon imaging; this block involved a mechanism iden

193 This imaging system, which we termed two-photon IMPRESS (imaging pathophysiology research sys

194 We used two-photon in vivo imaging to study neuronal integration

195 The combination of two two-photon-induced processes in a Forster resonance ener

196 can be catalytically assembled remotely via two-photon irradiation.

197 tracking larger populations of neurons using two-photon (jGCaMP7s,f) or wide-field (jGCaMP7c) imaging

198 ves the bR capacity for utilizing light upon two-photon laser excitation, thus paving the way to the

199 can generate an electrical signal under the two-photon laser excitation.

200 Here, using two-photon laser scanning microscopy (TPLSM) with an axi

201 We used two-photon laser scanning microscopy to visualize geneti

202 from three different microscopy modalities: two-photon laser-scanning microscopy, epifluorescence mi

203 imaging of evoked glutamate transients with two-photon laser-scanning microscopy.

204 nduce genomic modifications by one-photon or two-photon light induction in vivo.

205 approach may be implemented in multi-photon (two-photon) light-sheet fluorescence microscopy and, fur

206 Two-photon lithography (TPL)-based submicrometer additiv

207 Using a combination of two-photon lithography and high-temperature pyrolysis, w

208 Carbon plate-nanolattices are fabricated via two-photon lithography and pyrolysis and shown to reach

209 zinc-ion-containing aqueous photoresin using two-photon lithography and subsequently calcining them a

210 Two-photon live imaging demonstrates that the history of

211 Based on two-photon measurements of disparity tuning, the range o

212 rce, we produce hydrated electrons through a two-photon mechanism previously thought to be unimportan

213 ze their photolysis kinetics in both UV- and two-photon-mediated reactions; a two-photon action cross

214 In contrast to two-photon methods, however, one-photon methods suffer f

215 ure microscope in freely behaving mice and a two-photon microscope in head-fixed, unanesthetized mice

216 Here we present a 16-beam, two-photon microscope to monitor activity across the mou

217 To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that c

218 Here, we extend a two-photon microscope with an electrically tunable lens

219 s of near infrared (NIR) light provided by a two-photon microscope, or by a stand-alone laser during

220 Other than a traditional confocal or two-photon microscope, this approach requires no additio

221 cal neuronal activation by combining fUS and two-photon microscopy (2PM) in a co-registered single vo

222 ing with cellular resolution methods such as two-photon microscopy and electrophysiology is bridging

223 We used two-photon microscopy and followed NSCs that were geneti

224 monstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous a

225 By utilizing a variety of two-photon microscopy datasets, we show that our method

226 Intravital two-photon microscopy demonstrated that LN neutrophils w

227 olfactory and visual coupling, we conducted two-photon microscopy experiments in a new GCaMP6s-expre

228 We advance two-photon microscopy for near-diffraction-limited imagi

229 Two-Photon Microscopy has become an invaluable tool for

230 Here we used in vivo two-photon microscopy in awake head-fixed mice to assess

231 e directly observed microglia behaviors with two-photon microscopy in ex vivo spinal cord slices from

232 ith super-resolution imaging techniques, and two-photon microscopy in living knock-ins enables the vi

233 ond which three-photon microscopy outpeforms two-photon microscopy in recording fidelity.

234 Two-photon microscopy is a mainstay technique for imagin

235 Two-photon microscopy is used to image neuronal activity

236 Two-photon microscopy is widely used to investigate brai

237 ified by immunohistochemistry and intravital two-photon microscopy of kidneys of rats.

238 T cells using in vitro assays and intravital two-photon microscopy of lymphoid and nonlymphoid tissue

239 Entangled two-photon microscopy offers nonlinear imaging capabilit

240 We used two-photon microscopy to elucidate the spatial organizat

241 We used chronic in vivo two-photon microscopy to image dendritic spines and axon

242 Here, using two-photon microscopy to image neural activity and vascu

243 al network interactions in vivo by combining two-photon microscopy to monitor astrocyte calcium and e

244 We used two-photon microscopy to study the role of ensembles of

245 Here, we used in vivo two-photon microscopy to visualize surface AMPARs in mou

246 In addition, in vivo two-photon microscopy was performed for the first time o

247 Two-photon microscopy was used to observe mitochondrial

248 as that lie beyond the reach of conventional two-photon microscopy, which is typically limited to ~ 4

249 al morphology using widefield, confocal, and two-photon microscopy.

250 t in awake mice using subcellular-resolution two-photon microscopy.

251 expensive, making them promising sources for two-photon microscopy.

252 aster than point-scanning techniques such as two-photon microscopy.

253 d-type or eNMDAR loss-of-function mice using two-photon microscopy.

254 e imaged using epifluorescence, confocal, or two-photon microscopy.

255 itochondrial NADH utilization, measured with two-photon NAD(P)H fluorescence lifetime imaging (FLIM),

256 emical syntheses and require UV, visible, or two-photon NIR light to trigger release.

257 bjective is oriented above the brain and the two-photon objective is oriented horizontally, with imag

258 G; a nonlinear optical process that converts two photons of the same frequency into one photon of twi

259 al approach to drive behaviour with targeted two-photon optogenetic activation of small ensembles of

260 e, synchrony, and latency) using holographic two-photon optogenetic stimulation of olfactory bulb neu

261 tion, we describe strategies for single- and two-photon optogenetics that allow manipulation of the a

262 /3 of the primary visual cortex (V1), we use two-photon optogenetics to trigger action potentials in

263 simultaneous two-photon calcium imaging and two-photon optogenetics, we identified and selectively a

264 motion using polarography, spectroscopy, and two-photon phosphorescence lifetime measurements of oxyg

265 ts in the brain of awake mice, by performing two-photon phosphorescence lifetime microscopy at microm

266 High-intensity 730-nm light (two-photon photodamage) was used to cause single-cell da

267 to achieve intratumoral near-infrared (NIR) two-photon photodynamic therapy (PDT).

268 By combining time-resolved two-photon photoemission experiments with state-of-the-a

269 a three-dimensional hot electron gas through two-photon photoemission from a copper surface in vacuum

270 Here, femtosecond time-resolved two-photon photoemission spectroscopy has been used to p

271 ically-tunable defects in an LC device using two-photon polymerisation and explore the dynamic behavi

272 e present a novel biosensor design utilizing two-photon polymerisation and graphene to fabricate an e

273 zwitterionic photoresists are developed for two-photon polymerization 3D microprinting of hydrogel m

274 In particular, additive manufacturing with two-photon polymerization allows creation of intricate s

275 Our two-photon proposal encodes data points in a discrete, e

276 We study the Two Photon Quantum Rabi Model by way of its spectral fun

277 Second, when implementing two-photon quantum walks by exciting two superconducting

278 yield when immersed in water for 13 h, and a two-photon random laser based on the perovskite@silica n

279 excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with increased stability and

280 ion of a high-pressure sample chamber with a two-photon scanning microscope system, allowing for the

281 onal theory (TD-DFT) calculations, classical two-photon spectroscopy, and entangled two-photon spectr

282 sical two-photon spectroscopy, and entangled two-photon spectroscopy.

283 mary visual cortex (V1) by combining in vivo two-photon synaptic imaging and post hoc electron micros

284 Furthermore, these enzymes allow single-cell two-photon targeted genetic modifications and can be use

285 of three-dimensional confocal microscopy and two-photon time-lapse images of T cell-dendritic cell in

286 Whole-brain volumetric serial two-photon tomography (STPT) and a custom-developed imag

287 es were acquired with high resolution serial two-photon tomography and labeled plaques were detected

288 nterpolating high resolution in-plane serial two-photon tomography images with 100 mum z-sampling fro

289 -assisted energy transfer and enhancement of two-photon transitions.

290 its photochemical properties, especially its two-photon uncaging action cross section (delta(u)).

291 f juvenile rat brain (both sexes) by pairing two-photon uncaging of glutamate at spines and APs evoke

292 We find that unitary-like activation via two-photon uncaging of glutamate causes GC spines to rel

293 ain slices, thereby enabling high-resolution two-photon uncaging without any side effects.

294 A new approach to generate a two-photon up-conversion photoluminescence (PL) by direc

295 es are indeed responsible for generating the two-photon up-conversion PL in quasi-2D perovskites.

296 More importantly, the two-photon up-conversion PL is found to be sensitive to

297 n value leads to a dramatic decrease in the two-photon up-conversion PL signal.

298 Two-photon videomicroscopy revealed that a vast majority

299 A two-photon whispering-gallery mode laser device made of

300 We used a two-photon, whole-animal imaging paradigm that permitted