ライフサイエンスコーパス: unbind

1 protein folding/unfolding and ligand binding/unbinding).

2 e helical content, which were more likely to unbind.

3 a potent cellular toxin when intracellularly unbound.

4 Conversely, Xe, Kr, Rb and Cs are unbound.

5 ents but with slower kinetics of binding and unbinding.

6 consistent with slower glutamate binding and unbinding.

7 tional Mot1 in solution is necessary for TBP unbinding.

8 The unbound (18)F-fluoride fraction was determined in plasma

9 Equilibration between the blood and unbound (18)F-FMISO was rapid in all tumors.

10 trodes can be used directly to determine the unbound acetaminophen fraction without the need for any

11 artitioning, dissolution rate and release of unbound active drug.

12 is highly dynamic, with kinesin binding and unbinding along the length of the microtubule as it is t

13 requires local remodeling through crosslink unbinding and and/or filament disassembly.

14 rk elasticity and force-induced cross-linker unbinding and rebinding.

15 both allosteric and direct catalysis of GDP unbinding and release and GTP binding.

16 ystal structures of human IZUMO1 and JUNO in unbound and bound conformations.

17 ation showed a coherent allosteric mode from unbound and bound states, respectively.

18 uctures of full-length human PAH (hPAH) both unbound and complexed with BH(4) in the precatalytic sta

19 al structures of ISAV hemagglutinin-esterase unbound and complexed with receptor.

20 itative-quantitative composition in terms of unbound and esterified medium fractions.

21 the complexed virus and the presence of both unbound and expanded virus particles suggests receptor b

22 croscopy (cryo-EM) and crystal structures of unbound and H1-bound nucleosomes and validated these str

23 s assessed based on in-cell NMR signals from unbound and ligand-bound forms of a given DNA target.

24 assessed via rigid body simulations of both unbound and ligand-bound states of the protein.

25 structures in docking, 35% (6/17) when using unbound, and 12% (2/17) when using the homology-modeled

26 uni was first mixed with C. jejuni cells and unbound antibody was subsequently separated using a sequ

27 r-IR spectra of two forms of the protein, an unbound APO form and a HOLO form (where the PDZ1 is boun

28 ring the course of a reaction, involving two unbound apo structures, one ligand-bound intermediate, a

29 ctional nucleic acid, the SEO binding to the unbound aptamer triggered perturbations of both the inte

30 levant when transcription factor binding and unbinding are affected by epigenetic processes like DNA

31 which structural changes induced by agonist unbinding are transmitted within the human alpha7 nAChR.

32 Sequencing of IgA-bound and unbound bacteria revealed that before the onset of disea

33 ue to specific, molecular recognition, while unbound beads move along past the electrodes.

34 lts challenge conventional models of protein unbinding being unimolecular processes and independent o

35 g site within the N-terminal domain, into an unbound beta-barrel that interacts with the ribosome.

36 Median unbound BIC concentration was 2.48 ng/mL (IQR, 1.6-3.7).

37 Total and unbound BIC CSF concentrations were above the half-maxim

38 Protein-unbound bictegravir concentrations in SP, RT, and CVF hi

39 Total and protein-unbound bictegravir concentrations were quantified in BP

40 Unbound bilirubin had a nonlinear relationship to B(T) (

41 selectively measures free ionic bilirubin ("unbound" bilirubin - i.e., bilirubin not complexed to al

42 During unbinding, biotin crosses multiple energy barriers and v

43 ned the high-resolution crystal structure of unbound Brag2 containing the GEF (Sec7) and membrane-bin

44 ear kinesin head in the ATP waiting state is unbound but not displaced from its previous microtubule

45 e junction in a manner similar to when it is unbound, but there is no specificity in Mg(2+) binding f

46 es of bovine bestrophin-2 (bBest2) bound and unbound by Ca(2+) at 2.4- and 2.2- angstrom resolution,

47 evident that the genomes of many species are unbound by strict vertical descent: Large differences in

48 Thus, unbound cAMP maintains the PDE8-RIalpha complex while be

49 channel facilitates processive hydrolysis of unbound cAMP.

50 TR expression significantly in comparison to unbound Cas9/gRNA in HIV latent hmuglia/HIV (HC69) cells

51 d by a natural equilibrium between bound and unbound CENH3 (and its chaperones or binding proteins).

52 lectron and hole pairs, called excitons, and unbound charge carriers is a key cross-cutting issue in

53 culations and measurement of the fraction of unbound chemical, f(U)); (iv) species differences; and (

54 through diffusion, binding and orientation, unbinding, chemical transformations, and spatial localiz

55 n different molecular species, including the unbound, closed spike trimer, the fully open ACE2-bound

56 constant (k(ex)) of the imino protons in the unbound, cocaine-bound, and quinine-bound forms of the c

57 ogated by GBPs followed by the separation of unbound coded glycans.

58 at the RQC complex also exists as a ribosome-unbound complex during the escort of aberrant peptides t

59 The facilitated unbinding component likely occurs via a ternary complex

60 The impeded unbinding component likely results from Zur oligomerizat

61 We determined total and unbound concentrations of bictegravir (BIC) in cerebrosp

62 in a manner that is proportional to the free/unbound concentrations of the drug, thereby establishing

63 nd from Ser550 is sufficient to stabilize an unbound conformation of His583, as shown in a Asp576Ala

64 regulatory proteins adopt distinct bound and unbound conformations, and use this structural flexibili

65 e ratio of circ_Lrp6 bound to miR-145 versus unbound could play a role in vascular pathogenesis.

66 explained by the presence of both bound and unbound CT states in the same film, as a result of a ran

67 Moreover, after unbinding, CTCF quickly rebinds another cognate site unl

68 is with corresponding undetectable levels of unbound dabigatran.

69 Analysis of the unbinding data revealed a most probable interaction forc

70 ion that identical sequences may be bound or unbound depending on surrounding chromatin context and t

71 -thiolate/Ni(II) -disulfide (both bound and unbound disulfide-S to Ni(II) ) transformations via thiy

72 eparate bound DNA-biomolecule complexes from unbound DNA and determine the associated size change tha

73 ly separate the DNA-AuNP-labeled GO from the unbound DNA-AuNPs and the dissolved tissue matrices, the

74 Docking of protein domains to unbound double-stranded nucleic acids is used to generat

75 tin-bound Drp1 is significantly increased by unbound Drp1.

76 ant effect on many parameters that determine unbound drug exposure as well as drug potency.

77 Seminal protein-unbound DTG concentrations exceeded the in vitro 50% inh

78 study paves the way to investigations on the unbinding dynamics of more complex p38 inhibitors and ot

79 blies to reveal and characterize binding and unbinding dynamics within such assemblies.

80 riable discretization or coarse graining and unbinding dynamics, and although general assumptions tha

81 Allowing different unbinding energies not only has equilibrium effects on t

82 lapse to a universal curve regardless of the unbinding energies used or the identity of the dissociat

83 The unbinding energy per bond was found to be on the order o

84 LL4 catalyzed methylation of H3K4 at distant unbound enhancers via chromatin looping, which identifie

85 distorted DNA exists in low abundance in the unbound ensemble(3-9).

86 we report three crystal structures of SwaI: unbound enzyme, a DNA-bound complex with calcium ions; a

87 u-343/Arg-378 bridge is the default state in unbound ETS1 and maintained in high-affinity and nonspec

88 se timing sequence of stochastic binding and unbinding events allows one receptor to provide informat

89 tochastic process of innumerable binding and unbinding events between antibodies and the multiple bin

90 simulations, we observed over 70 binding and unbinding events between the alpha-helical molecular rec

91 nitor individual ligand:receptor binding and unbinding events in space and time by single-molecule im

92 We showed that the collective frequency of unbinding events in WT-RBCs is not significantly differe

93 Explore is able to observe millisecond-scale unbinding events using many nanosecond-scale trajectorie

94 nteractions are weak and undergo binding and unbinding events, but together they form a stable comple

95 intrinsic clearance in vitro to target high unbound exposure in vivo.

96 nd exhibit dramatically slower dynamics than unbound Fat4 and Ds1.

97 a priori not clear if they form a plasma of unbound fermionic particles or a gas of composite bosons

98 and SS-RBC membranes, as well as the median unbinding force between ICAM-4 and alphavbeta3.

99 However, the unbinding force between ICAM-4 and the corresponding lig

100 on cells, as measured by an increase in the unbinding force.

101 Depending on the loading rates, the unbinding forces between TIM and ligands ranged from 40

102 g on the direction of force application, the unbinding forces can more than triple.

103 We show that the unbound form of PopZ(Delta134-177) is unstructured in so

104 27 exhibits a limited binding surface in the unbound form, and stochastic conformational changes in D

105 mechanism for the catalyst in the bound and unbound form, whereas no significant production of CO wa

106 the structural differences between bound and unbound forms allow dehydrins to act as molecular shield

107 and the detailed structure of the bound and unbound forms are not known.

108 te moiety interconverts between Fc-bound and unbound forms, enabling enzymatic modification of the gl

109 x-ray crystal structures of ligand-bound and unbound forms, the activation of the erythropoietin rece

110 s were analysed to compare the antigen-bound/unbound forms.

111 capturing the structural flexibility in the unbound FP is challenging in the native Env trimer.

112 Until measurement of unbound fraction of calcineurin inhibitors becomes clini

113 domination by Enterobacteriaceae in the IgA-unbound fraction of the microbiota.

114 optimization of aggregate-binding affinity, unbound fractions in brain, permeability, and low efflux

115 d 28% of the BP concentration, while protein-unbound fractions were 51.1%, 44.6%, and 42.6%, respecti

116 proteolytically cleaved and released either unbound (free SC) or bound to immunoglobulins.

117 ent either in coreceptor-bound or coreceptor-unbound (free) forms, and we here present evidence that

118 Unbound ("free") bilirubin (B(f) ) was measured in patie

119 rug released from the NP mirrors that of the unbound, free-drug.

120 Here, we demonstrate that the presence of unbound/free ligand in colloidal suspension plays a pivo

121 sembles were capable of generating force but unbound frequently, resulting in slower contractile rate

122 the IF and OF conformations, the substrates unbind from the OF transporter and partition into the li

123 n complex that must continuously bind to and unbind from transiently exposed template strands during

124 , and it reverses within seconds of arrestin unbinding from the transient binding mode.

125 ly favored form with tyrosine protonated and unbound from the Cu(II) site, and an enthalpically favor

126 erties including the free fraction (fraction unbound (fu)) in plasma, and in vivo efficacy.

127 ed by one C atom simply from the bound to an unbound functionality and thus able to discriminate betw

128 pauses between binding events; the activator-unbound gene is not easily turned on again and activated

129 models to accurately discriminate bound and unbound genomic loci for both Cbf1p and Tye7p.

130 xperiments confirmed the disordered state of unbound HMGA2.

131 The roles of residual structure of unbound IDPs in coupling binding and folding have been u

132 report the high-resolution structure of the unbound IKKbeta-binding domain of NEMO that will greatly

133 from the gels while facilitating removal of unbound immunoprobe.

134 bited Vn to supported lipid bilayers, and to unbinding in freestanding lipid vesicles.

135 Further, since single particles are detected unbound in flow, the technique can be broadly applied to

136 Fraction unbound in plasma (fup) and intrinsic hepatic clearance

137 cules (such as switching between binding and unbinding) in the in vivo environment of living cells.

138 ing the specific DNA with the nonspecific in unbound, intermediate, and associated states, we found t

139 s convert cyclic nucleotide (CN) binding and unbinding into electrical signals in sensory receptors a

140 FkappaB is the strongest DNA binder, and its unbinding is controlled kinetically by molecular strippi

141 On one hand, binding (k'(on)) and unbinding (k(off)) rate constants were extracted from (1

142 simulations that incorporate fiber crosslink unbinding kinetics and continuum-scale simulations that

143 Experimental approaches to binding and unbinding kinetics are nowadays available, but we still

144 cts combined with rapid binding and moderate unbinding kinetics explain potent use-dependent block, w

145 pically also decay much more slowly than the unbinding kinetics of the indicator.

146 designing new drugs with engineered binding/unbinding kinetics.

147 Three-dimensional diffusion constants for unbound LacI computed from simulations closely match tho

148 ted from simulations closely match those for unbound LacI in vivo reported in the literature.

149 A possibly better picture of unbound LacI motion is that of gated diffusion, where DN

150 ults show that the dynamics of the bound and unbound LC13 TCR differ significantly.

151 ed when evaporating solvent, suggesting that unbound ligand and solvent occupy a similar space within

152 ands is not widely employed because residual unbound ligand continues to label newly synthesized Halo

153 ic resonance (NMR) to quantify the bound and unbound ligand populations.

154 importance of fully characterizing bound and unbound ligand populations.

155 amics simulations of single NCs confirm that unbound ligand readily swells the bound ligand shell ove

156 The controlled addition of unbound ligand represents a novel mechanism for directin

157 e of ligand binding and the concentration of unbound ligand, leading to a model-independent binding c

158 Results show that both unbound linker histones adopt a single compact conformat

159 y developed metadynamics-based protocol, the unbinding mechanism of an inhibitor of the pharmacologic

160 molecules in solution to participate in the unbinding mechanism.

161 ping velocities and provided evidence of the unbinding mechanisms.

162 Diffusion of unbound MeCP2 is strongly constrained by weak, transient

163 The amount of unbound Mirococept postperfusion was found to be within

164 e antibody-antigen pair ranking in bound and unbound models but recovers additional top-ranked cognat

165 ired in the native structure, presuming that unbound molecules would have lower indexes.

166 We hypothesized that unbound molecules, lacking the partnering structure of a

167 ld be less effective in separating bound and unbound molecules.

168 rms current methods in determining bound and unbound motif sites.

169 nt of the bound motors and attachment of the unbound motors.

170 The structure of unbound muPA revealed an unsuspected non-chymotrypsin-li

171 At heterotypic contacts with E-cadherin, unbound N-cadherin induces an asymmetric accumulation of

172 Plasma is a medium of unbound negative and positive particles with the overall

173 The structures of unbound NEMO show a closed conformation that partially o

174 mes a different conformation compared to the unbound NMR-based solution structure of the same PG-frag

175 voltammogram shape) suggests that the large unbound nucleic acid concentration may be altering the m

176 allow us to observe the dynamic binding and unbinding of 5-hydroxytryptamine (5-HT) (i.e., serotonin

177 quires dephosphorylation of the receptor and unbinding of arrestin, processes that are poorly underst

178 e into account the rates for the binding and unbinding of dopamine to D1 and D2 receptors.

179 lly tied to material damage via force-driven unbinding of fiber crosslinks.

180 potential involvement of TM5i in binding and unbinding of Na2, i.e. the Na(+) bound in the Na2 site,

181 In the latter case of cooperative unbinding of pairs, two neighboring Fis proteins on DNA

182 ing to impaired photoisomerization and rapid unbinding of photoisomerized cis-BI.

183 rescence to observe directly the binding and unbinding of short oligonucleotides (7-12 nt) to a compl

184 The interplay between binding and unbinding of synaptic receptor proteins at synapses play

185 h our previous work suggest that binding and unbinding of the AD to the Bateman domain dimer induces

186 Herein, the FES of unbinding of the antagonist N-(3alpha-hydroxy-5beta-chol

187 Unbinding of the cAMPs appears ordered as indicated by a

188 which allowed us to measure the force due to unbinding of the colloidal probe and the planar membrane

189 further unveil a sequence of events, whereby unbinding of the hydrolysis product (ADP + Pi) is follow

190 ule, rather than by reversing the asymmetric unbinding of the motor from the microtubule.

191 Derepression comes from unbinding of the regulator, which, under zinc-starvation

192 ple exchange of proteins and (2) cooperative unbinding of two Fis proteins to yield bare DNA.

193 Through a series of controlled additions of unbound oleic acid to solutions of identically sized ole

194 In IFCE, the unbound oligonucleotide does not reach the detector, imp

195 p of partitioning in which protein-bound and unbound oligonucleotides move in the opposite directions

196 thought to be critical for barrier function, unbound omega-O-acylceramide and bound omega-hydroxycera

197 ating the existence of a large population of unbound or wide-orbit Jupiter-mass planets (reported to

198 force CD81 into either the open (cholesterol-unbound) or closed (cholesterol-bound) conformation.

199 rsists for millennia relative to low-energy, unbound organic carbon.

200 Our results suggest that unbound p27 adopts a compact conformation and undergoes

201 quantitatively correlated with the amount of unbound PAH molecules in the AuNP suspensions, as judged

202 rtially bound state allows the protein to be unbound, partially bound, or fully bound to a DNA strand

203 weighted ensemble algorithm to determine the unbinding pathway for different poses of PK-11195, a TSP

204 provide a thorough description of the ligand unbinding pathway identifying the most stable binding mo

205 However, detailed information on the full unbinding pathway is often lacking due, in part, to the

206 when the force dependence of the facilitated unbinding pathway is stronger than that of the spontaneo

207 way is stronger than that of the spontaneous unbinding pathway.

208 tely reconstruct the spontaneous binding and unbinding pathways from nanosecond to second time-range.

209 ylated and phosphorylated ARH1, the possible unbinding pathways of ADP-ribose from non-phosphorylated

210 omplex to determine the binding strength and unbinding pathways over the widest dynamic range.

211 s, our modeling reveals stark differences in unbinding pathways.

212 We use umbrella sampling to cover a range of unbinding pathways.

213 We discover that while unbound PD-1 exhibits a hard-to-drug hydrophilic interfa

214 more than the number of currently available unbound peptide macrocycle structures.

215 tide digest of prolamins), the flow-through (unbound peptides), and the output (captured peptides) we

216 result in a major decrease in the number of unbound phosphorylated SH2 domain-binding sites.

217 Unbound piperacillin plasma concentration and fractional

218 Unbound planets can also be formed through gravitational

219 at accelerate the exchange between bound and unbound populations by orders of magnitude.

220 rt to the high tunability of their bound and unbound populations.

221 ber: cargos driven by a few motors tended to unbind prematurely from the microtubule, whereas cargos

222 provide an atomistic, dynamic picture of the unbinding process, replacing a simple two-state picture

223 rent energies for the different steps in the unbinding process.

224 of the active site play crucial roles in the unbinding process.

225 tifying the rate-limiting step of the ligand unbinding process.

226 er, it remains difficult to elucidate how an unbound protein diffuses inside the cell with good spati

227 ads with subsequent proteolytic digestion of unbound proteins and peptide-based phosphorylation enric

228 xposed to 100mg of mAzo and washed to remove unbound proteins by magnetic field.

229 Anchors that bind and unbind rapidly from matrix accumulate on nanoparticulate

230 From our simulations, we estimated the unbinding rate as koff = 0.020 +/- 0.011 s(-1).

231 Binding and unbinding rate constants measured across a wide range of

232 d that in live Escherichia coli cells, Zur's unbinding rate from DNA is sensitive to Zur protein conc

233 to the higher association rates and moderate unbinding rate of these two compounds with sodium channe

234 nucleoid-associated proteins that exhibit an unbinding rate that depends on the concentration of free

235 ein-DNA binding rate is much larger than the unbinding rate, a special case of fast promoter switchin

236 consequence, both the effective binding and unbinding rates for motors are much lower than the expec

237 rdered as indicated by a large difference in unbinding rates from the two sites, but the cause has re

238 cal method for estimating diffusion, binding/unbinding rates, and active transport velocities using F

239 Furthermore, because of slow unbinding rates, both receptor populations integrated do

240 as compared to the excited MLCT state of the unbound Re catalyst or when immobilized on ZrO2, which r

241 ions, we resolve two binding modes and three unbinding reaction pathways of a mechanically ultrastabl

242 ovide quantitative insights into binding and unbinding reactions among rapidly diffusing molecules th

243 approximately three times the volume of the unbound receptor.

244 ul predictions were made for 21 bound and 18 unbound receptors.

245 to be highly flexible in simulations of the unbound RecU, in agreement with SAXS observations.

246 f RNA secondary structure in the intervening unbound regions.

247 Binding and unbinding results in largely reduced cAMP dynamics, whic

248 ucture-based energetic calculations to model unbound RNA states.

249 Consequently, it is less clear how the unbound, sensing riboswitch refolds into the ligand bind

250 K(+)-channels in a unique example of binding/unbinding simplicity.

251 However, unbound soluble SAA is intrinsically disordered and is e

252 taneously concentrate and separate bound and unbound species in an assay based on electrical mobility

253 hydrins to act as molecular shields in their unbound state and as membrane protectants in their bound

254 The structural insights came from the unbound state and complexes of NagZ with the substrate,

255 ate process slows down the transition to the unbound state and favors rebinding, thus explaining the

256 port crystal structures of human Glce in the unbound state and of an inactive mutant, as assessed by

257 ative to the initial current measured in the unbound state of antibody.

258 ing-in-frame a conformation distinct from an unbound state or anti complex.

259 ysis of the fraction of DNA in the bound and unbound state to characterize binding behavior including

260 P1 conformation was studied in its bound and unbound state using mass spectrometry, small-angle X-ray

261 ntify three kinetically separated states (an unbound state, an encounter state, and the final complex

262 entially identical chemical potential in the unbound state, we reduced the problem of dissecting the

263 ), adopt a random-coil conformation in their unbound state.

264 ganded Env trimer or the gp41 peptide in the unbound state.

265 uctural flexibility of beta-importins in the unbound state.

266 -have been thoroughly characterized, but the unbound states have been more elusive.

267 lar electrostatic interactions influence the unbound states of PUMA only marginally.

268 es of the HCN1 channel in the cAMP bound and unbound states, the structural mechanism coupling ligand

269 indicators of the TF-DNA bound (on bead) and unbound states.

270 conversion between various protein bound and unbound states.

271 The unbound structure shows a high level of disorder and an

272 al region (NTR) previously unseen in our DNA unbound structure.

273 en complexes of which 11 have both bound and unbound structures available, and we generated a represe

274 n loop, and a possible self-locked state for unbound SUN.

275 sequence of a DARPin and a structure of the unbound target.

276 res within TCRs differ significantly between unbound TCR and TCR/pMHC simulations.

277 ed solvent accessible surface of the CDRs of unbound TCR simulations but also in (b) less expected re

278 he hydrogen bond network around CDR3alpha of unbound TCR simulations.

279 r low-affinity decoys, noise in the level of unbound TF always monotonically decreases to the Poisson

280 slows the time-scale of fluctuations in the unbound TF levels, the decay of bound TFs leads to faste

281 irst, IMPACT distinguishes between bound and unbound TF motif sites with high accuracy (average AUPRC

282 r arbitrary degradation rates for both bound/unbound TF states, we find rich noise behaviors.

283 iguity index is systematically smaller among unbound than bound molecules, as expected.

284 ormational changes in the process of binding/unbinding the transducer.

285 perpendicular to the pulling direction that unbinds the proteins and forms force-independent ideal b

286 tion of positive electric field successfully unbinds the thrombin from the aptamer.

287 sion increases faster than cross-linkers can unbind, the marginal band will coil, whereas if the tens

288 -cyclodextrin with RNTPs reveal two distinct unbinding time scales, which suggest that interactions o

289 uld allow the adhesin to undergo binding and unbinding to surface ligands under low force and remain

290 K(+) and Na(+), mainly unbound to any ligand in the sample, have been identifie

291 The binding and unbinding transitions of Vn at the membrane interface ar

292 We were able to reproduce the unbinding trends seen experimentally for both DNA and pr

293 In vivo, BI 749327 (30 mg/kg/day, yielding unbound trough plasma concentration ~180 nM) improves le

294 he local concentration of tPA through forced unbinding via degradation of fibrin and tPA release.

295 us with bacteria, followed by the removal of unbound virus, increased bacterial adherence to respirat

296 Estimation of unbound vitamin A in milk protein-Vit A complexes was ca

297 These complexes were further evaluated for unbound vitamin A, ability of milk protein to bind vitam

298 n-water interactions, and the most mobile to unbound water.

299 t of ribonucleoprotein (RNP) complexes, and "unbound," with physiological functions performed without

300 convertase and C3 convertase, as well as the unbound zymogen C2 obtained by small angle x-ray scatter