ライフサイエンスコーパス: uncage

1 S (laser-scanning photostimulation/glutamate uncaging).

2 scanning microscopy and two-photon glutamate uncaging.

3 e a greatly reduced calcium release upon Sph uncaging.

4 atterned stimulation by two-photon glutamate uncaging.

5 tude decrease in metal binding affinity upon uncaging.

6 diminish fluorescence of the VF dye prior to uncaging.

7 tropic and ionotropic receptors or direct UV-uncaging.

8 aser-scanning photostimulation and glutamate uncaging.

9 cells using two-photon imaging and glutamate uncaging.

10 yquinoline (BHQ)-based linker for two-photon uncaging.

11 py, in combination with two-photon glutamate uncaging.

12 p to 100 ms before or up to 500 ms after IP3 uncaging.

13 s for convenient quantitative calibration of uncaging.

14 ng studies, biochemical analysis and InsP(3) uncaging.

15 fferent emission properties before and after uncaging.

16 ough voltage-gated Ca(2+)-channels or Ca(2+) uncaging.

17 rrents, evaluated using two-photon glutamate uncaging.

18 rase IX, and this biomarker was harnessed to uncage a potent cytotoxic agent.

19 umarin, which in turn triggers hydrolysis to uncage a target molecule.

20 sed and continuous patterns into the flow by uncaging a fluorescent dye, highly detailed measurements

21 emical properties, especially its two-photon uncaging action cross section (delta(u)).

22 s, so that chromophores with high two-photon uncaging action cross-sections (delta(u)) can be designe

23 arization responses to brief (1 ms) nicotine uncaging adjacent to IPR neurons.

24 IP3 uncaging also triggers oscillatory Ca(2+) release, but,

25 Here we use glutamate uncaging and a novel optogenetic strategy to track chang

26 ircuit mapping using 2-photon (2P) glutamate uncaging and analyze experience-dependent neonatal devel

27 nical aspects of performing neurotransmitter uncaging and channelrhodopsin-assisted circuit mapping,

28 Here we have used two-photon glutamate uncaging and compartmental modeling to reveal a gradient

29 Using two-photon glutamate uncaging and dendritic patch-clamp recordings, we found

30 near the channels, in response to glutamate uncaging and depolarization, respectively.

31 uses a standard 2P microscopy setup for both uncaging and detection and a set of lithographically mad

32 cellular resolution, using two-photon Ca(2+) uncaging and dynamic-clamp.

33 ly, NPE-HCCC2 and HCCC2 have high two-photon uncaging and excitation efficiency, respectively, enabli

34 f electrophysiological, two-photon glutamate uncaging and imaging methods, we show that mature indivi

35 fficiency, respectively, enabling two-photon uncaging and imaging to be combined to examine cell coup

36 NPs as probes for controlled localization of uncaging and imaging with multiphoton z-axis sectioning.

37 ted with MeCP2 deficiency, we used glutamate uncaging and laser scanning photostimulation to survey i

38 Here, we established presynaptic Sr(2+) uncaging and made quantitative Sr(2+)- and Ca(2+)-imagin

39 synaptically connected cell pairs, NPEC-AMPA uncaging and miniature current recordings.

40 nzyme activity (host-guest interactions with uncaging and molecular cleavage).

41 cell recordings, two-photon microscopy, GABA uncaging and optogenetics to study dendritic inhibition

42 e, laser scanning photostimulation glutamate uncaging and photoactivation of channelrhodopsin-2 were

43 ts (ICa(V)) with UV-light flash-induced Ca2+ uncaging and presynaptic Ca2+ imaging to study the Ca2+

44 We characterized the mechanism of uncaging and the effect of 2-AG on the regulation of the

45 Using two-photon glutamate uncaging and time-lapse imaging of rat hippocampal CA1 n

46 Here, we use two-photon glutamate uncaging and time-lapse imaging to show that non-ionotro

47 d presynaptic activity (two-photon glutamate uncaging and two-photon imaging of the FM 1-43 assay, re

48 Using glutamate uncaging and two-photon imaging, we demonstrate that the

49 ser scanning microscopy with glutamate photo-uncaging and whole-cell recording to examine synaptic st

50 ent probes, most of which rely on the photo-"uncaging" and photoisomerization reactions.

51 otoreactive groups to allow photoliberation (uncaging) and photo-cross-linking in a sequential manner

52 sing dendritic recording, 2-photon glutamate uncaging, and computational modeling, we investigated ho

53 siological, two-photon imaging and glutamate uncaging, and electron microscopic assays in acute brain

54 o stimulate presynaptic neurons by glutamate uncaging, and mapped the location of sites that provide

55 cell electrophysiology, two-photon glutamate uncaging, and optogenetics in TC slices containing the a

56 ing pharmacological manipulations, glutamate uncaging, and two-photon imaging of GFP-transfected hipp

57 -photon laser scanning microscopy, glutamate uncaging, and whole-cell electrophysiological recordings

58 e the versatility of this general sequential uncaging approach by applying it to control Wip1 phospha

59 sed this question by applying the photolytic uncaging approach to induce focal increases in Ca2+ leve

60 a indicate that oscillations elicited by IP3 uncaging are driven by the biphasic regulation of the IP

61 (2+) wave velocity, whereas responses to IP3 uncaging are enhanced.

62 that allows wavelength-selective two-photon uncaging at 900 nm versus 720 nm.

63 endritic spines were stimulated by glutamate uncaging at a diffraction-limited spot, and the localiza

64 vesicle priming by using presynaptic Ca(2+) uncaging at a small, glutamatergic, central synapse.

65 ing combined with two-photon laser glutamate uncaging at apical spines of CA1 pyramidal neurons to ex

66 We used direct stimulation by calcium uncaging at identified, single-site inhibitory synapses

67 Two-photon (2p) glutamate uncaging at individual dendritic spines showed that E2 i

68 Using focal glutamate uncaging at individual synapses, we find only a subpopul

69 r an action potential with near-simultaneous uncaging at multiple spines.

70 dal neurons in acute brain slices, glutamate uncaging at single spines showed that iGluSnFR responds

71 However, brief glutamate uncaging at spines on distal dendrites evoked somatic up

72 r Colo357 cells during light-controlled H(+) uncaging at the hypoxic core.

73 gonal catalysis by producing xenobiotics and uncaging biomolecules in living systems.

74 Importantly, prior to uncaging, bis-CNB-GABA is inactive at the GABAA receptor

75 se inhibitor vorinostat that was efficiently uncaged by heterogeneous Pd catalysis in cell culture mo

76 We used glutamate uncaging by laser scanning photostimulation to map the l

77 matic core that can be selectively reduced ("uncaged") by one cell type, liberating a luciferin that

78 a Nikon A1R confocal microscope, was used to uncage Ca(2)(+) or IP3 and conduct photobleaching experi

79 We found that uncaging Ca(2+) activated biphasic BK currents with fast

80 uffer exchange and a pulsed laser system for uncaging caged compounds.

81 aged cyclic RGD peptide and demonstrate that uncaging can be efficiently performed with biologically

82 Furthermore, it has been shown that PIP(2) uncaging can trigger rapid, syt1-dependent exocytosis in

83 Uncaging caused Ca2+ levels to increase not only in the

84 Focal glutamate uncaging confirms MNTB neurons as a source of inhibitory

85 Postphotolysis, uncaged copper promotes hydroxyl radical formation under

86 d with time-lapse live imaging and glutamate uncaging, could detect plasticity-related changes in spi

87 cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notc

88 rties of BODIPY dyes lead to photocages with uncaging cross sections over 10000 M(-1) cm(-1), values

89 number of channels participating in the GABA uncaging current and an increase in receptor desensitiza

90 Furthermore, multisite two-photon glutamate uncaging demonstrated that HCN channels control the ampl

91 ulation or to approximately 300 nM by Ca(2+) uncaging dilated parenchymal arterioles in control brain

92 g GABA synthesis via intracellular glutamate uncaging dramatically potentiated GABA release within 1

93 nsity, as expected for a chemical two-photon uncaging effect.

94 a, although their heads are activated by the uncaging event, as determined with calcium imaging.

95 ectively on spines and act locally to dampen uncaging-evoked Ca transients and somatic potentials.

96 es showed that E2 increases the amplitude of uncaging-evoked EPSCs (2pEPSCs) and calcium transients (

97 ignificantly increased the amplitude of both uncaging-evoked EPSPs (uEPSPs) and spine Ca transients.

98 ndrites, we explored plasticity of glutamate uncaging-evoked excitatory input patterns with various s

99 stimulate individual spines while monitoring uncaging-evoked excitatory postsynaptic potentials (uEPS

100 Uncaging-evoked excitatory postsynaptic potentials and C

101 branches while simultaneously recording the uncaging-evoked excitatory postsynaptic potentials and l

102 Uncaging-evoked potential amplitudes correlated inversel

103 hese findings, we also show rapid, glutamate-uncaging-evoked, time-locked BDNF release from single de

104 Data from glutamate-uncaging experiments and simulations indicate that mGlu2

105 Single spine glutamate-uncaging experiments confirmed that less than half of th

106 Gradual intracellular Ca(2+) uncaging experiments revealed that exocytosis had a simi

107 Multiphoton glutamate uncaging experiments revealed that the increase in dendr

108 Multiphoton glutamate uncaging experiments revealed that the increase in dendr

109 Two-photon glutamate uncaging experiments show somatic depolarization enhance

110 Combining uncaging experiments with mathematical modeling, we were

111 a computational model validated by glutamate uncaging experiments.

112 Using Ca(2+) imaging, glutamate uncaging, fluorescence recovery after photobleaching and

113 apply a novel two-component Aggregation and Uncaging Focused Ultrasound Sequence (AU-FUS) at the des

114 Photolytic H(+) uncaging from 2-nitrobenzaldehyde also raised [Ca(2+)]i,

115 treatment with FC led to potentiation of the uncaged GABA response, suggesting nucleus-specific roles

116 uR5 can signal from intracellular membranes, uncaging glutamate on a CA1 dendrite led to a local Ca(2

117 Uncaging glutamate over whole-cell patch-clamped cells i

118 We activated inputs at targeted locations by uncaging glutamate sequentially to generate apparent mot

119 rom the dissipative spread of photolytically uncaged H(+) ions across cytoplasm.

120 Real-time Ca(2+) imaging and Ca(2+) uncaging here reveal that CDF turns out to be strikingly

121 Synaptic mapping by glutamate uncaging identified layer 2/3 as the main source of loca

122 However, results using glutamate uncaging implicated Ca(2+) influx through SNX-482-sensit

123 This new prodrug is effectively uncaged in cancer cell culture by Pd nanosheets captured

124 OT can be activated in neuron cell bodies or uncaged in dendrites to enable structural tracing via "b

125 oked by inositol-1,4,5-trisphosphate (InsP3) uncaging in airway SMCs.

126 synaptic terminals, and two-photon glutamate uncaging in dendritic spines performed in brain slices t

127 ty in normoxic conditions and small molecule uncaging in hypoxia.

128 responses evoked with focal glutamate photo-uncaging in the presence of TTX.

129 synaptic transmission and responses to GABA uncaging in the thalamic reticular nucleus (nRT) that is

130 macological stimuli or cell-specific calcium uncaging in vascular smooth muscle cells or astrocytes.

131 perturb Ca(2+) oscillations elicited by IP3 uncaging, indicating that reloading of endoplasmic retic

132 ive effects on AMPAR recycling and glutamate uncaging-induced structural and functional plasticity.

133 H(+) uncaging into buffer mixtures in vitro demonstrated that

134 neurons, but had no effect on AHPs evoked by uncaging intracellular Ca(2+) .

135 The mechanism of the photoinduced uncaging involves homolytic C-C bond fragmentation follo

136 Ca(2+) waves could also be evoked by uncaging IP(3) with a UV flash in the dendrites.

137 could be generated by Ca(2+) waves evoked by uncaging IP(3), showing that other signalling pathways a

138 confined to the main apical dendrite because uncaging IP3 in the oblique dendrites has no effect on t

139 had no effect on Ca(2+) increases induced by uncaging IP3.

140 -yl)-propyl-1-phosphonic acid and CNQX or by uncaging IP3.

141 The final uncaging is accomplished within 32 mus.

142 pines in response to low-frequency glutamate uncaging is saturable, reversible, and requires NMDA rec

143 , and photochemical release of glutamate (or uncaging) is a chemical technique widely used by biologi

144 reduce short-term facilitation when GABA is uncaged just prior to the onset of stimulation.

145 ing CAPS-proteins, suggesting that PI(4,5)P2 uncaging may bypass CAPS-function.

146 calculations, we can characterize the entire uncaging mechanism and identify the most relevant interm

147 we used patch-clamp, imaging, and glutamate uncaging methods in rat olfactory bulb slices to test fo

148 r GABA in its natural state using two-photon uncaging microscopy for the first time.

149 Furthermore, uncaging of 1,5-(PP)(2)-InsP(4) but not 3,5-(PP)(2)-InsP

150 ngs, elevation of intracellular Ca(2+) by UV uncaging of 1-(4,5-dimethoxy-2-nitrophenyl)-EDTA-potenti

151 After uncaging of 2-AG, we monitored calcium levels, CB1-GIRK

152 ventricles of live zebrafish by means of the uncaging of a fluorescein derivative.

153 membrane-associated dimeric protein with the uncaging of a powerful cytotoxic.

154 f techniques by demonstrating the mechanical uncaging of a reactive species within a single protein.

155 ances the templated reaction and enables the uncaging of a substrate.

156 Uncaging of bis-CNB-GABA evokes inward GABAergic current

157 Localized photolytic uncaging of Ca(2+) from o-nitrophenyl-EGTA in somatic ER

158 r NP-EGTA, the UV flash photolysis-catalysed uncaging of Ca(2+) generated CICR in only 9% of the beta

159 c myocytes, where either one- and two-photon uncaging of Ca(2+) induced highly local or cell-wide phy

160 Here, we utilize the photo-uncaging of Ca(2+) with CaV 2.1 channels fluxing Li(+) c

161 nd FAK inhibits repulsive turning from focal uncaging of Ca(2+) within filopodia.

162 Rapid uncaging of Co(2+) ions by patterned UV light activates

163 photon active at 720 nm, optically selective uncaging of DEAC450-caged biomolecules at 900 nm may all

164 Two-photon uncaging of DEAC450-Glu at 900 nm at spine heads on pyra

165 Uncaging of DMn is considerably faster, but DMn has a si

166 ution using optically independent two-photon uncaging of each neurotransmitter.

167 exhibited direct (non-synaptic) responses to uncaging of excitatory and inhibitory transmitters.

168 Using uncaging of GABA, there is a decreasing GABAergic influe

169 his electrical filtering, we used two-photon uncaging of glutamate and compared the integration of el

170 photon imaging, optogenetics, and dual-color uncaging of glutamate and GABA, we demonstrate that plat

171 t is accomplished using UV laser-based photo-uncaging of glutamate and imaging neuronal activation by

172 rat brain (both sexes) by pairing two-photon uncaging of glutamate at spines and APs evoked by somati

173 that unitary-like activation via two-photon uncaging of glutamate causes GC spines to release GABA b

174 Laser-scanning photo-uncaging of glutamate focally activated neurons in layer

175 d two recently developed methods, two-photon uncaging of glutamate for determining the EPSC of indivi

176 ly different methods: (i) UV light-triggered uncaging of glutamate in intact cells or (ii) piezo-driv

177 Moreover, activation of MOPP cells by focal uncaging of glutamate induced strong inhibition of granu

178 Using two-photon uncaging of glutamate on spine heads from mouse layer-5

179 ) on depolarizations generated by two-photon uncaging of glutamate on spines from mouse neocortical p

180 ut screening method exploiting laser-induced uncaging of glutamate to construct excitatory input maps

181 Here, we use two-photon laser uncaging of glutamate to directly activate glutamate rec

182 Focal uncaging of glutamate, accomplished by switching a pulse

183 ation of EPSPs, generated through two-photon uncaging of glutamate, this action was largely shunted b

184 ser scanning microscopy and two-photon laser uncaging of glutamate, we show that SK channels regulate

185 ser-scanning microscopy and two-photon laser uncaging of glutamate.

186 g iontophoresis of l-aspartate or two-photon uncaging of glutamate.

187 Uncaging of glycerophosphoryl-myo-inositol 4,5-bisphosph

188 ereas postsynaptic stores (activated by spot-uncaging of inositol 1,4,5-trisphosphate) remain unaffec

189 ith UDP-GlcNAc had an attenuated response to uncaging of InsP3.

190 eover, the Ca(2+) responses to melatonin and uncaging of IP(3) were mutually exclusive in infected RB

191 ow-intensity tetanic synaptic stimulation or uncaging of IP3 increased the decay time of spontaneous

192 Furthermore, uncaging of MNI glutamate reveals that thalamocortical n

193 Two-photon (2P) uncaging of MNI-glutamate onto individual spines suggest

194 ary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing.

195 Photolytic uncaging of p-hydroxyphenacyl (pHP) GABA demonstrates ba

196 of presynaptic GABA receptors by photolytic uncaging of RuBi-GABA has a biphasic effect on EPSC ampl

197 (520 mum) by excitability testing and focal uncaging of RuBi-GABA on the axon at varying distances f

198 patch clamp electrophysiology and photolytic uncaging of RuBi-GABA we show that GABA(B) receptors are

199 le-cell patch-clamp recording and photolytic uncaging of RuBi-GABA.

200 Thus, optical uncaging of signaling lipids can uncover their rapid eff

201 Further, uncaging of Sph leads to the translocation of the autoph

202 micropatterns by means of photolithographic uncaging of surface amines.

203 on as well as pinpoint the location at which uncaging of the molecules occurred.

204 aling lipids sphingosine and diacylglycerol; uncaging of the probe for these two species triggered ca

205 In addition, through uncaging of the same compound, gene expression is contro

206 Finally, uncaging of these protected peptides by either UV or two

207 a rapid decrease in pH that accompanies the "uncaging" of an effector molecule from o-nitrobenzaldehy

208 ectron chromophore for efficient multiphoton uncaging on living neurons.

209 e, simulation of cortical input by glutamate uncaging on proximal dendritic spines produced potential

210 ing combined with two-photon laser glutamate uncaging onto CWC spines.

211 mouse model of fragile X syndrome, glutamate uncaging onto individual dendritic spines yields stronge

212 Patterned uncaging opens new vistas in the study of signal integra

213 ecific LTP induction by two-photon glutamate uncaging or by synaptic stimulation, subthreshold stimul

214 cular smooth muscle cells via ex vivo Ca(2+) uncaging or in vivo optical activation produced only poo

215 Glutamate uncaging or optogenetic stimulation of cortical spheroid

216 lor experiments, e.g., when combining Ca(2+) uncaging or optogenetic stimulation with Ca(2+) imaging

217 fluorescence recovery after photobleaching, uncaging or photoactivation/switching as well as single-

218 city protocol, in which two-photon glutamate uncaging over a spine is paired with postsynaptic spikes

219 ramidal cells, we find that the amplitude of uncaging potentials at the soma is inversely proportiona

220 lly localized to the spine and occurred with uncaging potentials of different amplitudes and in spine

221 Uncaging potentials onto spines summed linearly, whereas

222 In practically all spines examined, uncaging potentials were significantly reduced by TTX.

223 nal chromaffin cells, we show that PI(4,5)P2 uncaging potentiates exocytosis and identify synaptotagm

224 The uncaging process is compatible with biological milieu an

225 marin chromophore influences the rate of the uncaging process, opening the way to exploiting these ne

226 ficant affinity for Mg(2+) to complicate the uncaging process.

227 Here, we describe unique uncaging properties displayed by two coumarin-caged thym

228 In response to a glutamate uncaging pulse, CaMKIIalpha-CaM association increases in

229 Mechanistic studies of the uncaging reaction implicate a photoredox pathway involvi

230 Here we report a near-IR light-initiated uncaging reaction sequence based on readily synthesized

231 in we report a novel bioorthogonal tetrazine uncaging reaction that harnesses tetrazine reactivity to

232 We present herein an uncaging reaction that results from ring-closing metathe

233 Bioorthogonal uncaging reactions offer versatile tools in chemical bio

234 However, the precise mechanism of such uncaging reactions remains elusive.

235 no effect on cytoplasmic [H(+)] in the H(+)-uncaging region, indicating that DIDS-sensitive transpor

236 Nicotine uncaging revealed nAChR functional enhancement by cNIC o

237 in a single spine using two-photon glutamate uncaging, RhoA and Cdc42 were rapidly activated in the s

238 ed immolative linker by Ru(II) conjugates to uncage rhodamine was achieved using different oligomeric

239 ere significant increases in the fraction of uncaging sites from which EPSCs could be evoked ("hot sp

240 ices showed that the proportion of glutamate uncaging sites from which excitatory postsynaptic curren

241 xially unsymmetrical silicon phthalocyanines uncage small molecules preferentially in a low-oxygen en

242 sprouting, a higher proportion of glutamate-uncaging spots in the granule cell layer evoked EPSCs in

243 enlargement resulting from a high-frequency uncaging stimulus into spine shrinkage, demonstrating th

244 Bioorthogonal uncaging strategies have recently emerged as an experime

245 A bioorthogonal uncaging strategy is presented, which is triggered by he

246 ared to the conventional methods, this novel uncaging system requires no external light source and sh

247 We applied this BRET uncaging system to release a potent kinase inhibitor, ib

248 for the alignment and calibration of a focal uncaging system.

249 Low-energy light prompts photo-uncaging (t1/2 <1-2 min) and target-specific modificatio

250 Photochemical uncaging techniques use light to release active molecule

251 Uncaging technology and fluorescence microscopy are 'opt

252 al to its cleavage peptide and blue light to uncage the cleavage site.

253 The second sequence uncages the carrier's cargo locally to achieve high targ

254 small plant protein domain, such that light uncages the G-protein activating module.

255 This platform technology uncages the therapeutic power of mAbs for various CNS di

256 cantly diminished affinity for Cu2+, thereby uncaging the metal ion.

257 using brief, targeted exposure to UV light, uncaging the rhodamine and causing the particles in that

258 Phenol-containing small molecules are uncaged through sequential release of the C4'-amine and

259 We used two-photon glutamate uncaging to activate NMDA-Rs on individual dendritic spi

260 th local electrical stimulation or glutamate uncaging to analyze the effect of MOR activation on loca

261 We have used multisite two-photon glutamate uncaging to deliver different spatiotemporal input patte

262 enetics, two-photon microscopy and glutamate uncaging to examine D2R-dependent modulation of glutamat

263 Using two-photon microscopy and glutamate uncaging to examine individual synapses in the rat stria

264 -photon microscopy, and two-photon glutamate uncaging to examine subthreshold synaptic integration in

265 We used two-color, two-photon uncaging to fire and block action potentials from rat hi

266 e lifetime imaging with two-photon glutamate uncaging to image the activity of the small guanosine tr

267 Here, using two-photon glutamate uncaging to induce plasticity at individual dendritic sp

268 d somatic recordings and multisite glutamate uncaging to investigate the relationship between synapti

269 aser scanning photostimulation via glutamate uncaging to map excitatory and inhibitory synaptic input

270 We used two-photon RuBi-Glutamate uncaging to optically map how the largest population of

271 We then use local pharmacology and GABA uncaging to show that dendritic GABA(B)Rs also decrease

272 Here we use 2-photon glutamate uncaging to stimulate individual spines while monitoring

273 Additionally, uncaging trains caused a reduction in the Ca(2+) accumul

274 Low-frequency uncaging trains induced Ca(2+)-dependent long-term depre

275 Finally, PI(4,5)P2 uncaging triggered the rapid fusion of a subset of readi

276 have developed an approach for sequentially uncaging two different phosphopeptides in one system, en

277 ng visual receptive field mapping, glutamate uncaging, two-photon Ca(2+) imaging, and genetic labelin

278 ng 3D digital holography and focal glutamate uncaging, voltage-sensitive dye, two-photon imaging, ele

279 To probe biological utility, thiol group uncaging was carried out using a peptide derived from th

280 This increase of calcium caused by calcium uncaging was followed by recovery to the prestimulated l

281 rpine-treated rats, laser-scanning glutamate uncaging was used to randomly and focally activate neuro

282 gher extinction coefficient at the preferred uncaging wavelength.

283 By applying patterned, two-photon glutamate uncaging, we found that single dendrites of cortical pyr

284 o quantify pH values upon UV irradiation and uncaging, we introduce a simple silica nanoparticle pH s

285 Using local uncaging, we photolyzed dextran-CANPE-HCC to release the

286 nique in combination with 2-photon glutamate uncaging, we show that neurofibromin, in which loss-of-f

287 Using two-photon uncaging, we show that this subcellular targeting strong

288 Using two-photon glutamate uncaging, we stimulated nascent spines on dendrites of r

289 Using two-photon glutamate uncaging, we then reveal that GABA(B) receptors strongly

290 s evoked within approximately 100 ms of GABA uncaging were increased, while EPSCs evoked approximatel

291 s evoked approximately 300-600 ms after GABA uncaging were reduced compared to interleaved control sw

292 aged calcium probe o-nitrophenyl EGTA and UV uncaging were used to increase calcium in endocytic vacu

293 During repetitive glutamate uncaging, which induces spine structural plasticity, CaM

294 an enhanced version of two-photon glutamate uncaging, which preserves inhibitory synaptic transmissi

295 We then used two-photon glutamate uncaging, whole-cell recording, and Ca(2+) imaging to an

296 e show that drugs and fluorescent probes are uncaged with fast rates, including in the presence of ma

297 GTA, which has a negligible Mg(2+) affinity, uncages with a time constant of 10.3 ms, resulting in re

298 ne assays, RNA-Seq, and two-photon glutamate uncaging with calcium imaging, we show that knocking dow

299 Here we combine two-photon uncaging with two-photon imaging of a fluorescent label

300 thereby enabling high-resolution two-photon uncaging without any side effects.