ライフサイエンスコーパス: unfolding

1 globin, followed by cofactor loss and globin unfolding.

2 t are resistant to both charge migration and unfolding.

3 structures with respect to each other during unfolding.

4 mechanisms underlying SC identity are still unfolding.

5 unt largely for understanding the process of unfolding.

6 orces applied to a biomolecule result in its unfolding.

7 is commonly initiated by partial or complete unfolding.

8 he shape and size of proteins during folding/unfolding.

9 n and previous measurements of apohemoglobin unfolding.

10 the flagellar export channel prior to their unfolding.

11 on had a destabilizing effect on the protein unfolding.

12 induced nonideal SEC behavior and/or protein unfolding.

13 raordinarily stable against dissociation and unfolding.

14 itochondrial inner membrane without previous unfolding.

15 entified as an integral character of protein unfolding.

16 counteraction of denaturant-induced protein unfolding.

17 stabilisation of the protein complex against unfolding.

18 change, which stabilizes the protein against unfolding.

19 ed alpha-syn destabilizes the G4Q leading to unfolding.

20 les in orchestrating tissue growth are still unfolding.

21 e basis of one-dimensional models of protein unfolding.

22 ic regions exposed to solution after protein unfolding.

23 and makes Red Broccoli resistant to thermal unfolding.

24 specific regions of the protein that undergo unfolding.

25 aged complex that is competent for substrate unfolding.

26 that is not accounted for by denaturation or unfolding.

27 rowding is able to partially rescue G4Q from unfolding.

28 mines which receptors are needed for maximal unfolding ability, and, in some cases, simultaneous enga

29 rate-mediated activation of the proteasome's unfolding ability.

30 o ubiquitin chains activate the proteasome's unfolding ability?

31 Upon exposure to pulling forces, MSD unfolding accelerates exponentially over a force scale o

32 , and fluorescence analyses revealed protein unfolding, aggregation, and structural rearrangement as

33 Unfolding along this path is required for mtClpX to gate

34 ivity in part by mediating bacterial protein unfolding and aggregation.

35 ysical characterization of processes such as unfolding and aggregation.

36 of almond milk proteins was assessed, as the unfolding and association of these proteins in response

37 while subsequent wet heating induced partial unfolding and de-aggregation.

38 alternative clipping pathway that precludes unfolding and degradation; the UBL degron favors degrada

39 ing affinity, monitoring protein folding and unfolding and determining hidden conformational changes

40 oteins have key functionalities encompassing unfolding and disassembly of such substrates in differen

41 roxide dismutase (SOD1) dimers take over the unfolding and dissociation free energy landscape in a va

42 n in inflammation sites; however, mechanical unfolding and exposure to forces >6 pN block thioester r

43 Stress-induced unfolding and fibrillation of insulin represent serious

44 ficient nonequilibrium processes through the unfolding and folding of single DNA hairpins.

45 ein stability is related to the ratio of the unfolding and folding rates, this increase completely ac

46 main highly susceptible to ANGPTL4-catalyzed unfolding and inactivation.

47 pair of coupled equilibria (protein folding/unfolding and ligand binding/unbinding).

48 ering them highly susceptible to spontaneous unfolding and loss of enzymatic activity.

49 chemical prodrugs addresses issues with RNA unfolding and nanoparticle dissociation after high-densi

50 To probe intermediate states during unfolding and oligomerization of proteins remains a majo

51 ime-resolved luminescence method for protein unfolding and PLI detection with nanomolar sensitivity.

52 equilibrium constants for both G-quadruplex unfolding and POT1 binding to the resultant single-stran

53 76% and 94% respectively), suggesting domain unfolding and preference for 'end-on' oriented binding,

54 nning calorimetry, enable studies on protein unfolding and protein-ligand interactions (PLI).

55 ichia coli ClpAP undergoing active substrate unfolding and proteolysis.

56 higher ion concentrations to display GQ-like unfolding and refolded via intermediates, contrary to th

57 by a 10-fold decrease in the rates of enzyme unfolding and refolding as well as a reduction of the in

58 We present unfolding and refolding data for the small, single-domai

59 ated by Rep involves repetitive cycles of G4 unfolding and refolding fueled by ATP hydrolysis.

60 ulation studies of the temperature dependent unfolding and refolding of a titin immunoglobulin domain

61 ecular mechanisms controlling the mechanical unfolding and refolding of proteins cannot be accessed b

62 which exhibited very high energy barriers of unfolding and refolding).

63 c, energy-intensive mechanism to promote RNA unfolding and refolding, suggesting an evolutionary conv

64 mation on substrate states during ATP-driven unfolding and subsequent proteolysis in solution.

65 We studied the influence of BSA unfolding and those of two Hofmeister salts, sodium chlo

66 he ClpA double-ring hexamer powers substrate unfolding and translocation into the ClpP proteolytic ch

67 from specific recognition into a nonspecific unfolding and translocation machine.

68 ide variety of sequences to ensure efficient unfolding and translocation of almost any native substra

69 enosine triphosphate (ATP)-fueled mechanical unfolding and translocation steps that lead to processiv

70 mechanisms of translocation-coupled protein unfolding and translocation.

71 ssessed by lower transition temperatures for unfolding, and three of these manifested defects in subs

72 od subdomains are stabilized by force during unfolding, and US confirmed these results.

73 pecifically those with a high free energy of unfolding are, in fact, so stable that they exist only i

74 d alphaA-crystallin and destabilized against unfolding, are active chaperones and can transfer the di

75 eedles into intestinal tissue using a set of unfolding arms.

76 C, but is completely inactivated by protein unfolding at higher temperatures.

77 domain show pronounced differences in their unfolding behavior, underlining the fact that the choice

78 ng transition suggests a classical two-state unfolding behavior.

79 and found that they differ in stability and unfolding behavior.

80 homodimers (i.e., they are not more prone to unfolding), but they remain highly susceptible to ANGPTL

81 f state of myosin II, for its activation and unfolding by phosphorylation, and for understanding the

82 n cbEGF domains uniquely orchestrate protein unfolding by showing that MFS disulfide bond mutations m

83 nvironments by shifting the onset of protein unfolding by ~100 MPa.

84 hort-degron protein substrates, we show that unfolding can occur directly from the initial closed-cha

85 targeted unfolding contrasts with the global unfolding canonically executed by ClpX homologs and prov

86 k to question the scientific response to the unfolding changes.

87 Moreover, we measured markedly different unfolding characteristics for spectrin repeats within th

88 he results reveal the mechanistic details of unfolding, characterizing structure of intermediates eve

89 We find that rather than simply unfolding chromatin, histone hyperacetylation results in

90 source optics for improved collision induced unfolding (CIU) experiments and an electromagnetostatic

91 Collision-induced unfolding (CIU) has emerged as a valuable technique for

92 Ion mobility (IM)-based collision-induced unfolding (CIU) has gained increasing attention to probe

93 tive ion mobility (IM) and collision induced unfolding (CIU) mass spectrometry (MS).

94 e ion mobility MS (IM-MS), collision-induced unfolding (CIU), and hydrogen-deuterium exchange MS (HDX

95 -ion mobility (IM)-MS with collision-induced unfolding (CIU), chemical labeling using N-ethylmaleimid

96 We find that substrate unfolding constitutes the rate-limiting step during tran

97 This targeted unfolding contrasts with the global unfolding canonicall

98 avirus 2 (SARS-CoV-2) has caused the rapidly unfolding coronavirus disease 2019 (COVID-19) pandemic(1

99 Using unfolding curves at varying ligand concentrations, our "

100 e not observed, we estimate that each global unfolding cycle consumes ~10 ATPs.

101 arlier findings that ~90% of global ribozyme unfolding cycles lead back to the kinetically preferred

102 when designing experiments and interpreting unfolding data.

103 d-to-protein ratio (LPR) near 10 and partial unfolding detectable by Trp59 fluorescence occurring at

104 In contrast to apparent cooperative unfolding detected with denaturant-induced and thermal-i

105 domains are completely different in terms of unfolding energies and number of transitions.

106 The chemical denaturant reduces the unfolding enthalpy DeltaH(cal) and lowers the midpoint t

107 veal a pretransition at 72 degrees C with an unfolding enthalpy of DeltaH(cal) ~200-300 kcal/mol and

108 ch partially compensates the endothermic mAb unfolding enthalpy.

109 wth rate, in real-time over the course of an unfolding epidemic.

110 hese interventions, based on modeling of the unfolding epidemic.

111 This unfolding event enables an autoinhibitory compact H-NS c

112 However, how this unfolding event is triggered for proteins that are other

113 ce remains nearly constant after the initial unfolding event, and a few pathways and a high degree of

114 he force drops to near zero after each major unfolding event, and numerous intermediates are observed

115 on prompted dimers to transition through two unfolding events and dissociate symmetrically into monom

116 These observations can shed more light on unfolding events for RRM-containing proteins.

117 g theory for the quantitative description of unfolding events of large, therapeutic proteins, such as

118 on and exhibits a rich variety of reversible unfolding events that are augmented when the Ca(2+) conc

119 It is suggested that unfolding events within the TTR monomer originate at the

120 unclear how the memory systems structure the unfolding experience into discrete event units that can

121 Both unfolding experiments and independent small angle x-ray

122 with denaturant-induced and thermal-induced unfolding experiments and stopped-flow refolding studies

123 lity-mass spectrometry and collision induced unfolding experiments have recently garnered attention a

124 Kinetic unfolding experiments monitored by fluorescence spectros

125 ly unfolded states by Mg(2+), as observed in unfolding experiments, with this stabilization being due

126 conformational protection as well as protein unfolding/folding kinetics.

127 The observation of two-state unfolding for many small single-domain proteins by denat

128 Unfolding free energy profiles determined by BXD suggest

129 es up to ~300 MPa: either a complete protein unfolding, from native dimers to Gaussian chains, or a p

130 force range relevant for protein folding and unfolding, from which single-molecule kinetic and thermo

131 approach to develop strategies to combat the unfolding global outbreaks of C. auris and other skin-as

132 izes the kinase domain, thereby altering the unfolding hierarchy.

133 s that are associated with events initiating unfolding in DBD are likely to present opportunities for

134 This reveals a heightening of the barrier to unfolding in G37R by >5 kJ/mol(-1) over the other varian

135 ysis reveals a common (pan-neuronal) program unfolding in highly coordinated fashion in all neurons,

136 redicted to poise p53 on the cusp of folding/unfolding in the cell, with a major determinant being av

137 for capturing high-speed biological dynamics unfolding in three dimensions.

138 ly toxic to myeloma cells by causing protein unfolding, increasing further the substrate load on prot

139 tramolecular cross-links, leading to polymer unfolding instead of mechanochemical events at the backb

140 Recently, unfolding intermediates separated by as few as two amino

141 ontrast to small single-domain proteins, mAb unfolding is a complex reaction that is analyzed with th

142 For the investigated mAb, unfolding is characterized by a cooperativity parameter

143 number of guanidineHCl molecules bound upon unfolding is deduced from the DSC thermograms.

144 ndergoing remodeling by mtClpX revealed that unfolding is limited to a region extending from the mtCl

145 d on several iMs that exhibited fast folding/unfolding kinetics.

146 evelopment of biotherapeutics, where protein unfolding leads to adverse properties, such as aggregati

147 Unfolding leads to the exposure of a hydrophobic beta-st

148 ned, these results support a charge-directed unfolding mechanism for protein complexes.

149 o fully characterize the complex folding and unfolding mechanism of multidomain proteins, it is neces

150 vels produce smaller clutches by a chromatin unfolding mechanism.

151 We proposed a possible "refolding-after-unfolding" mechanism, as further supported by monitoring

152 Although our knowledge of the unfolding mechanisms and the magnitude of the forces inv

153 epending on BLG concentration, two different unfolding mechanisms are observed in situ under pressure

154 ially coherent scenes that form the basis of unfolding memory events.

155 ed an ingestible capsule, termed the luminal unfolding microneedle injector, which allows for the ora

156 G4 formation (folding), stabilization, and unfolding must be regulated to coordinate G4-mediated bi

157 ments also indicated that the charge at the "unfolding" N-terminus of ADH decreased at high in-source

158 Their unfolding occurs on the luminal side of the membrane by

159 sed excitability, which promotes accelerated unfolding of a neural 'script' for task execution, despi

160 The thermal unfolding of a recombinant monoclonal antibody IgG1 (mAb

161 -42) is electrostatically driven and induces unfolding of Abeta(M1-42).

162 el in which conditional disorder-the partial unfolding of ACDs upon monomerization-is a common mechan

163 ions of pressure drops capture refolding and unfolding of all three mutants by a similar mechanism, t

164 ictions from critical theory on the temporal unfolding of avalanches have yet to be confirmed in vivo

165 ility to measure the dynamics of the initial unfolding of bacteriorhodopsin provides a platform for q

166 ectric fields contribute to distinct folding/unfolding of beta-lactoglobulin, resulting in structural

167 that water molecules play in the mechanical unfolding of biomolecules has not yet been fully elucida

168 n important role in the mechanically induced unfolding of biomolecules.

169 In this report, a stepwise unfolding of bovine serum albumin (BSA) with guanidine h

170 BI prevents thermal unfolding of Broccoli at 37 degrees C, leading to more f

171 menon can be explained by the combination of unfolding of disordered domains and breaking of native c

172 olecular dynamics simulations the mechanical unfolding of dystrophin's spectrin repeat 1 and related

173 ild denaturing conditions indicates that the unfolding of each immunoglobulin domain can be explained

174 Local unfolding of either betaB-betaD or helix 2 can generate

175 the recognition, chaperone dissociation and unfolding of exported T3SS proteins.

176 l transition of peptide assemblies mimic the unfolding of fibronectin, thus enabling formation of sph

177 l, we identified 31 intermediates during the unfolding of five helices of EF-cleaved bR.

178 sigma ~6 x 10(-5) and a Gibbs free energy of unfolding of g(nu) ~100 cal/mol per amino acid.

179 ke on G4-DNA, DHX36 displays ATP-independent unfolding of G4-RNA followed by ATP-dependent refolding,

180 It further accelerates the unfolding of helices and a complete collapse of structur

181 the protein cavity, but only the fast local unfolding of helix 2 is relevant to the ligand entry pro

182 Furthermore, unfolding of inner membrane cristae is coupled to change

183 n of conserved cysteine residues and partial unfolding of its structure convert the heat shock protei

184 IM-MS platforms, allowing for comprehensive unfolding of large multiprotein complex ions as well as

185 ation of LPL, accomplished by catalyzing the unfolding of LPL, does not require the conversion of LPL

186 o 5D2-bound LPL counteracts ANGPTL4-mediated unfolding of LPL.

187 ined increasing attention to probe gas-phase unfolding of proteins and their noncovalent complexes, n

188 Such analyses revealed unfolding of proteins on the mineral surface and an incr

189 Our modeling data suggest that the complete unfolding of proteoforms during CZE separation benefits

190 Aptamer binding caused partial unfolding of rPrP(90-231) and modulated its ability to u

191 e, beany flavours were mitigated through the unfolding of secondary structure of PPI by forming solid

192 provides a novel candidate mechanism for the unfolding of serotonin's antidepressant effects over tim

193 at the molecular level induces shear-induced unfolding of single proteins and can drive their assembl

194 e the scope and limitations of S1-chaperoned unfolding of structured RNAs.

195 hniques and provide free energy profiles for unfolding of talin rod subdomains.

196 o revealed that CYG molecules caused partial unfolding of the BSA structure, while it was not enough

197 e that the M125Q mutation causes a localized unfolding of the C-terminal lobe of CaM, preventing the

198 we show that human body temperature promotes unfolding of the central dimerization domain, breaking u

199 V sensor to the bZIP effector independent of unfolding of the connecting A'alpha-helix, an observatio

200 the energy barrier, and therefore assist in unfolding of the flagellar proteins before feeding them

201 xtraction and that Hp binding prevents local unfolding of the Hb heme pocket, leaving IsdH unable to

202 in-binding site is unfolded at ~6 pN; and 3) unfolding of the M1 domain is necessary for high-affinit

203 Mechanical unfolding of the MSD activates GPIb-IX signaling into pl

204 ults indicate that upon mechanically induced unfolding of the protein, the solvent molecules become m

205 gy barrier for unfolding, resulting in rapid unfolding of the protein.

206 m form of human telomeric DNA, it causes the unfolding of the same G4 sequence in sodium buffer.

207 molecular dynamics (MD) simulations to study unfolding of the talin rod.

208 Instead, we found that the unfolding of the target site structure plays a key role

209 r distinct tRNA species implicates transient unfolding of the terminal RNA helix during nanopore tran

210 ores, and their formation can lead to severe unfolding of VSDs from the channel.

211 Given the slow unfolding of what may become catastrophic changes to Ear

212 ion with measurements of the rate of RNase H unfolding on and off the ribosome, their results show th

213 ith: space-filling 'side-on' orientation and unfolding on CH(3) SAM; greater numbers of FN molecules

214 e proteins often result in gas-phase protein unfolding or loss of noncovalent interactions.

215 engue serotypes in Puerto Rico and a rapidly unfolding outbreak of plague in 2017 in Madagascar.

216 unique vocal elements patterned in sequences unfolding over hours.

217 ogical, and molecular evidence to propose an unfolding paradigm in which old age, chronic metabolic d

218 completely unfolded, following two different unfolding paths: using acid or guanidinium chloride (Gdm

219 ur alpha-helices of ACBP and showed that the unfolding pathway is marginally affected by the ligand.

220 Mg(2+)-RNA interactions that occur along an unfolding pathway of the Twister ribozyme.

221 d can be explained by either a change in the unfolding pathway or as a reflection of a multidimension

222 lations, we identify intermediates along the unfolding pathway, which provide buffering to allow exte

223 ed to obtain more detailed collision-induced unfolding pathways for Cytochrome C.

224 protein complex ions through their distinct unfolding pathways in the gas phase.

225 thering our understanding on the folding and unfolding pathways of retinal-binding proteins and membr

226 e-profile analysis, to probe the folding and unfolding pathways of RNase H (RNH) nascent chains stall

227 oporphyrin IX) disassociation and apoprotein unfolding pathways.

228 The unfolding patterns associated with the F(ab')(2) domains

229 Upon IdeS-digestion, the unfolding patterns of the F(ab')(2) and Fc domains were

230 The unfolding process does not require direct adsorption of

231 after a first, to our knowledge, successful unfolding process that represents a point of no return a

232 omain can be explained by a simple two-state unfolding process, with different unfolding rate for eac

233 dynamic properties of interaction during the unfolding process.

234 tide chain has a substantial impact on which unfolding properties are observed by single-molecule For

235 The enthalpy of unfolding provides the number of amino acid residues nu

236 d that ribosome-stalled RNH has an increased unfolding rate compared with free RNH.

237 uanidinium chloride were used to extrapolate unfolding rate constants and half-lives of the crystalli

238 two-state unfolding process, with different unfolding rate for each domain.

239 This behavior indicates an unfolding rate that increases exponentially with the app

240 higher forces, however, the increase of the unfolding rate with the force becomes faster than expone

241 elaborate on the differences in folding and unfolding rates in nucleic acid quadruplexes.

242 ition, provide estimates of mRNA folding and unfolding rates in vivo, and provide experimental eviden

243 The results indicate that a folding-unfolding reaction of a specialized temperature-sensitiv

244 increases the activation free energy for the unfolding reaction of ACBP without affecting the positio

245 ich POT1 binding is coupled to an obligatory unfolding reaction, is the most plausible mechanism.

246 amino acid residues nu participating in the unfolding reaction.

247 few as 10 tandem hairpins displayed ensemble unfolding/refolding transitions, which were exploited to

248 Backbone HDX is mediated by opening/closing (unfolding/refolding) fluctuations.

249 ts as an axonal tension buffer by reversibly unfolding repeat domains of the spectrin tetramers to re

250 the propensity for Coulombic repulsions and unfolding/restructuring, helping to preserve the native-

251 to a lowering of the free-energy barrier for unfolding, resulting in rapid unfolding of the protein.

252 s at ambient pressure, NMR-detected pressure unfolding revealed significant deviation from two-state

253 rix diagram can be used to study folding and unfolding routes.

254 al predictions optimize our interaction with unfolding sensory events.SIGNIFICANCE STATEMENT Temporal

255 the linguistic information contained in the unfolding sentences modulates brain activity in a word-s

256 ising protocol, we detect separate gas-phase unfolding signatures for lipid and protoporphyrin TSPO b

257 The unfolding signatures of both domains were allowed to cor

258 re the diagnostic domain to provide specific unfolding signatures to differentiate IgG subclasses and

259 ations, which deploy enhanced sampling, with unfolding simulations at high temperatures.

260 and extrapolating transition rates from the unfolding simulations to lower, physiologically-reasonab

261 ECD fragments indicated unfolding started at the N-terminus, and the charge stat

262 some context to help decision-making in the unfolding story of COVID-19.

263 ing different chemical biology-based protein unfolding systems, we reveal the segregation of unfolded

264 ant domains (Calpha/Cbeta) that increase the unfolding temperature of Calpha/Cbeta by 20 degrees C, i

265 enaturant molecules and is more sensitive to unfolding than the main transition.

266 roved to be 50-60 kJ/mol less stable against unfolding than their pilus rod-like counterparts (which

267 ferent pesticides was carried by folding and unfolding the filter paper-based structure, without any

268 After completely unfolding the protein at high force, we lower the force

269 Conformational IgE epitopes were removed by unfolding these proteins through reduction and alkylatio

270 time was 5.9 +/- 1.1 minutes; and mean graft unfolding time was 2.9 +/- 0.9 minutes.

271 outcome measures were graft preparation and unfolding times, best spectacle-corrected visual acuity

272 as can cause structural changes ranging from unfolding to subunit dissociation.

273 tively explained by S1-dependent directional unfolding toward the downstream RBS.

274 The resulting zigzag unfolding trajectories occupied 40% more states per traj

275 e these measurements, conducting hundreds of unfolding trajectories using our fast Upside algorithm f

276 challenging, in part because any individual unfolding trajectory only occupies a subset of the total

277 G37R, which proceeded only through the first unfolding transition and displayed a much higher abundan

278 pment of the interdomain distance during the unfolding transition suggests a classical two-state unfo

279 mperature (chosen to be temperature near the unfolding transition).

280 present a study following denaturant-induced unfolding transitions of yeast phosphoglycerate kinase b

281 process; and (iii) maintaining the number of unfolding transitions.

282 petitive mechanism and also slows subsequent unfolding/translocation of these substrates as well as o

283 rent techniques can be used to study protein unfolding under tension.

284 Such dynamic fluctuation of rG4 folding-unfolding underpins the biological significance of these

285 Thermal unfolding was further studied in the presence of guanidi

286 How these ligands influence BLM-mediated hGQ unfolding was investigated using two independent single-

287 Although helix destabilization and/or unfolding was not observed at the initial epsilon-cleava

288 By utilizing the characteristic protein unfolding we can induce reversible shape-memory behavior

289 at the effects of electric fields in protein unfolding were linearly dependent on the applied electri

290 The swaps of beta-strands proceed via global unfolding, whereas the alpha-helices are free to swap lo

291 lium-in DMEK requires minimal time for graft unfolding, which is the surgical step considered most ch

292 secondary structures that resist mechanical unfolding while minimizing the amount of computational r

293 ings indicate that the ribosome promotes RNH unfolding while the nascent chain is proximal to the rib

294 e concentration of FA and the free energy of unfolding with a slope of m (FA+pH) (the combined contri

295 artially folded intermediates during protein unfolding with different secondary and ternary conformat

296 Abrupt GQ unfolding with K(+) in solution occurred at as many as f

297 tive dimers to Gaussian chains, or a partial unfolding with oligomerization in tetramers mediated by

298 However, "refolding-after-unfolding" with increasing in-source activation was not

299 d that POT1 binding is coupled to quadruplex unfolding, with a final complex with a stoichiometry of

300 ECD provided orthogonal evidence of monomer unfolding within this complex.