ライフサイエンスコーパス: unglycosylated

1 falciparum proteins that remain essentially unglycosylated.

2 rounding positions at 199, 222, and 298 were unglycosylated.

3 about half of triadin-1 in the heart remains unglycosylated.

4 the sugar binding site is mutated or CPY* is unglycosylated.

5 to the medium in a hydrophilic form that was unglycosylated.

6 glycosylation, induced the appearance of the unglycosylated 115-kDa CaR form, which was further incre

7 Similarly, unglycosylated ACET synthesized in HeLa cells, by using

8 ariant is not significantly altered over the unglycosylated analogue.

9 ing prion diseases, in which a patient's own unglycosylated and anchorless PrP is used to inhibit PrP

10 The auto-Abs neutralized both unglycosylated and glycosylated IFNs.

11 ull-length human PrP (rHuPrP23-231) (that is unglycosylated and lacks the glycophosphatidylinositol a

12 in E. coli and Pichia pastoris, resulting in unglycosylated and mannosylated proteins, respectively.

13 cted to have molecular masses of ca. 61 kDa (unglycosylated) and high isoelectric points (pI 9.3-9.5)

14 Cealpha2beta5 is unglycosylated, as are many other alpha2beta5 hormones.

15 kidney) identified six glycosylated and one unglycosylated Asn.

16 ibodies raised against both glycosylated and unglycosylated baculovirus-expressed RII recognized P. f

17 in the lateral membrane were substituted by unglycosylated beta(1) subunits displayed a decreased ab

18 it ACET cDNA resulted in the synthesis of an unglycosylated but inactive protein.

19 triadin-1 that indicated active breakdown of unglycosylated, but not glycosylated, triadin-1.

20 uced PrP fragments, namely, glycosylated and unglycosylated C2, within different central nervous syst

21 s, normalized to viral particles measured by unglycosylated capsid protein VP5 content).

22 less affinity were processed equally well by unglycosylated cathepsin D-HEL and HEL-Ld.

23 In contrast, the unglycosylated cathepsin D-HEL was retained in ER/Golgi

24 We prepared unglycosylated cellular prion protein (PrP(C)) substrate

25 s indicates that the rate of delivery of the unglycosylated channel to the cell surface is slower tha

26 e wild-type glycosylated and the N259Q+N263Q unglycosylated channels.

27 e significantly more lytic compared with the unglycosylated control.

28 sed thermostability in comparison with their unglycosylated counterparts expressed in Escherichia col

29 were found to be higher than those of their unglycosylated counterparts from RNase A.

30 t Pichia pastoris and compared them to their unglycosylated counterparts produced in Escherichia coli

31 ction, and blocks apparent Ly-49A binding to unglycosylated Dd.

32 Circular dichroism of unglycosylated diptericin indicated that the peptide lac

33 y spectrum was compared to that of synthetic unglycosylated drosocin, which shares strong sequence ho

34 the third glycosylation site available, was unglycosylated, enzymatically inactive and rapidly degra

35 Thus, secretion of glycosylated and unglycosylated ferritin is a regulated and not a stochas

36 he presence of SubAB- or tunicamycin-induced unglycosylated FLAG-tagged LCN2.

37 unogenic structure even when expressed in an unglycosylated form in Escherichia coli.

38 ifferent acid hydrolases: cathepsin D, to an unglycosylated form of cathepsin D, and to pepsinogen.

39 The smaller unglycosylated form of chondromodulin-I was only observe

40 the ompF promoter region, as compared to the unglycosylated form of OmpR.

41 the ERAD of alphaENaC was selective for the unglycosylated form of the protein.

42 mobility of approximately 19-21 kDa for the unglycosylated form, and a ragged N terminus between res

43 ters (including helix I), in glycosylated or unglycosylated form, in combination with the expression

44 al of mature chondromodulin-I and a smaller, unglycosylated form, were identified using antipeptide a

45 on disease by favoring the production of the unglycosylated form.

46 s shown that the amounts of glycosylated and unglycosylated forms of LHBs and MHBs proteins are all g

47 quantitative sensitivity of glycosylated and unglycosylated forms of LHBs and MHBs proteins, with sel

48 TCR responded to diversely glycosylated and unglycosylated forms of mycoketide when presented by DCs

49 With this system, both glycosylated and unglycosylated forms of pro-alpha factor were stable thr

50 at 6 h and was expressed in glycosylated and unglycosylated forms.

51 30, derive from both glycosylated as well as unglycosylated forms.

52 l formed in vitro spontaneously by a 9.7-kDa unglycosylated fragment of the human prion protein.

53 The unglycosylated G generated high protective immunity and

54 his study suggests that bacterially produced unglycosylated G protein could be developed alone or as

55 The bacterially produced unglycosylated G protein could be developed as a protect

56 rotective efficacy of the RSV A2 recombinant unglycosylated G protein ectodomain (amino acids 67 to 2

57 rotective efficacy of the RSV A2 recombinant unglycosylated G protein ectodomain produced in E. coli

58 Therefore, bacterially produced unglycosylated G protein holds promise as an economical

59 Moreover, p22(phox) coprecipitated with unglycosylated gp91(phox) primary translation product ma

60 ssion from glycosylated EP-procyclins to the unglycosylated GPEET-procyclin.

61 lex between the Ly49A NK receptor domain and unglycosylated H-2D(d).

62 As distinct from vIL-6, unglycosylated hIL-6 is as potent as glycosylated hIL-6

63 sponds to the calculated molecular weight of unglycosylated hSPO.

64 ral isoforms of glycosylated EP, but not the unglycosylated isoform EP2.

65 pulation of CD4(+) T cells that expressed an unglycosylated isoform of IL-4.

66 ion, PrP(M) showed impaired transport of the unglycosylated isoform to the cell surface.

67 Unglycosylated K10 bound to wild type but not psrP defic

68 at the post-translational site, resulting in unglycosylated KCNE1 subunits that cannot reach the cell

69 The expression of a fully acylated, unglycosylated lipid A indicates that lipid A biosynthes

70 s showed similar binding of glycosylated and unglycosylated lipid A to CD14/TLR4-MD-2.

71 ve bacteria, can survive and synthesize only unglycosylated lipid A.

72 g experiments showed that newly synthesized, unglycosylated M protein interacted with N protein in a

73 as many sites for gp70 binding as cells with unglycosylated mCAT-1.

74 ete intermediates that are not observed when unglycosylated molecules are degraded.

75 lular populations of the MUC5AC polypeptide (unglycosylated monomer and dimer, GalNAc-substituted dim

76 l-length receptor complex and a recombinant, unglycosylated MSD in isolation.

77 e turnover rates of wild-type protein and an unglycosylated mutant (N259Q,N263Q) were compared in pul

78 n Deltascj1 cells, the transport rate for an unglycosylated mutant carboxypeptidase Y (CPY) is marked

79 -type mCAT-1 and complete down-modulation of unglycosylated mutant mCAT-1.

80 Expression of the unglycosylated mutant on the cell surface, assessed usin

81 quantitatively similar in the wild-type and unglycosylated mutant proteins.

82 In contrast, the unglycosylated mutant was rapidly degraded (t(1/2) = app

83 ll fraction of wild-type Rem-CT, but not the unglycosylated mutant, was colocalized with Rab5-positiv

84 Consistent with this, we observe that unglycosylated N259Q+N263Q mutant channel forms oligomer

85 ontrast, the heterodimer formed by expressed unglycosylated Na,K-ATPase beta(1) subunit and the endog

86 dicated that megakaryocyte NMDAR1 was either unglycosylated or only glycosylated to low levels, and o

87 enzymatically active ACET proteins which are unglycosylated or underglycosylated.

88 al membrane glycoprotein, whereas F13L is an unglycosylated, palmitylated protein with a motif that i

89 d glycopeptides (mono- or disaccharides) and unglycosylated peptide controls were measured in human r

90 The unglycosylated peptide was purified to homogeneity by co

91 t CRT molecules can be complexed in vitro to unglycosylated peptides and used to elicit peptide-speci

92 osylation, we have prepared glycosylated and unglycosylated peptides derived from the 175-195 fragmen

93 Conversely, unglycosylated peptides did not exhibit this grouping be

94 The unglycosylated PK-resistant PrP(Sc) of CWD migrated at 2

95 the disulfide bridge favor formation of the unglycosylated prion protein.

96 on of glycosylation led to the production of unglycosylated pro-alpha factor (p alpha F), a processed

97 to a non-hydroxylated residue yielded mostly unglycosylated protein and a small fraction of mono-N-gl

98 R interaction is the first example of a GAL1/unglycosylated protein interaction in the extracellular

99 um of the GAL1/glycan lattice involving GAL1/unglycosylated protein interactions.

100 eering glycosylation motifs into a naturally unglycosylated protein, we are able to bring about site-

101 a tool of discriminating human PSA from the unglycosylated protein, with a limit of detection of 0.6

102 Moreover, mice expressing unglycosylated PrP did not develop clinical disease, and

103 Most importantly, we found that, in vivo, unglycosylated PrP does not acquire the characteristics

104 isfolded PrP typing showed a dominant 19 kDa unglycosylated PrP fragment (type 2) in all brains, foca

105 ion of diglycosylated, monoglycosylated, and unglycosylated PrP in spreading of infectivity to the br

106 rafficking to the cell membrane, whereas the unglycosylated PrP localization is mainly intracellular.

107 Both RML- and 301C-derived prions containing unglycosylated PrP(Sc) molecules were infectious to wild

108 produce RML and 301C mouse prions containing unglycosylated PrP(Sc) molecules.

109 on and by differing ratios of di-, mono- and unglycosylated PrP(Sc).

110 Conversion of unglycosylated PrP-sen appeared to alter both the amount

111 tructured at low concentrations, recombinant unglycosylated PrP106 (rPrP106) undergoes a concentratio

112 The results show that unglycosylated PrPC molecules are required to propagate

113 bited by substoichiometric levels of hamster unglycosylated PrPC molecules.

114 The unglycosylated PrPCTF 12/13 migrate at 12 and 13 kDa and

115 s of PrPM reach the cell surface whereas the unglycosylated PrPM is also under-represented in the bra

116 Normal levels of RDS and the unglycosylated RDS binding partner rod outer segment mem

117 Unglycosylated recombinant gpA inhibited secretion, sugg

118 ies: ELISA and Western blotting assays using unglycosylated recombinant hLAMP-2 expressed in Escheric

119 hways of amyloid formation in vitro, we used unglycosylated recombinant PrP corresponding to the prot

120 but was insensitive to BFA, suggesting that unglycosylated Rem-CT does not reach this BFA-dependent

121 way, and drastically delays maturation of an unglycosylated reporter protein in the RER.

122 afety and protective efficacy of recombinant unglycosylated RSV G protein ectodomain produced in E. c

123 tion of complexes containing a dimer of SCF (unglycosylated SCF1-165) plus two molecules of sKit.

124 Functional expression of the unglycosylated SERT mutant, SERT-QQ, was also increased

125 oteasome inhibitors cause accumulation of an unglycosylated, signal peptide-bearing form of PrP on th

126 Unglycosylated Sil1 is not only functional but is more e

127 an ER protein can exist in the cytosol as an unglycosylated species and impair cellular functions.

128 sent study, we have identified an additional unglycosylated species, with an apparent molecular mass

129 nnel alpha subunits (bCNGA3) convert from an unglycosylated state, at 90 kDa, to two glycosylated sta

130 selective binding of PS1 holoprotein to the unglycosylated subunit and the Sec61alpha/oligosaccharyl

131 hese systems indicated that mEHg was largely unglycosylated, suggesting that expression in the ER was

132 eptide of 100 amino acids, representing five unglycosylated tandem repeats, presented by dendritic ce

133 stantial increases in the relative levels of unglycosylated triadin-1, proteasome inhibition led to a

134 t channels in HEK293 cells we found that the unglycosylated TRPM8 mutant (N934Q) displays marked func

135 Both N-glycosylated and unglycosylated TRPV1 was detected in extracts of periphe

136 his protein while Ect SMCs elaborate only an unglycosylated type II TGF-beta receptor.

137 at Tyr(369)(N) fails to be presented because unglycosylated tyrosinase is degraded rapidly and relati

138 glycosylation, compromising the delivery of unglycosylated V0a1 to lysosomes, ultimately impairing V

139 ses of 38-54 (glycosylated), 30, and 27 kDa (unglycosylated) were apparent.

140 Both FGFR2C278F and unglycosylated wild type FGFR2 signal through phospholip

141 not required for proper targeting, although unglycosylated wild-type receptors did not localize prop

142 cipitation of beta(1) was also less with the unglycosylated YFP-beta(1) than with glycosylated YFP-be

143 -precipitation both with rat YFP-beta(1) and unglycosylated YFP-beta(1) was associated with decreased