ライフサイエンスコーパス: unnatural amino acid

1 utilizing protein expressed with an alkynyl unnatural amino acid.

2 voltage-clamp fluorometry with a fluorescent unnatural amino acid.

3 labeled enzyme are largely unaffected by the unnatural amino acid.

4 age C-H oxidation to one containing a linear unnatural amino acid.

5 ent structure (7) was replaced by natural or unnatural amino acids.

6 or efficiently designing peptides containing unnatural amino acids.

7 post-translational modifications (PTMs) and unnatural amino acids.

8 rature procedures for the syntheses of these unnatural amino acids.

9 ll molecules bearing natural or bioisosteric unnatural amino acids.

10 rocyclic peptides that contain a majority of unnatural amino acids.

11 he engineering of translation to incorporate unnatural amino acids.

12 r facilitate the expression of proteins with unnatural amino acids.

13 which are the most frequently used to encode unnatural amino acids.

14 e beta-barrel as well as the introduction of unnatural amino acids.

15 y in live cells by genetically incorporating unnatural amino acids.

16 eins, and expand the genetic code to include unnatural amino acids.

17 brary (HyCoSuL), which uses both natural and unnatural amino acids.

18 n through the site-directed incorporation of unnatural amino acids.

19 photostabilizer and biomolecular target via unnatural amino acids.

20 ish eight tripeptides, each having different unnatural amino acids.

21 nalogues in which Phe(13) was substituted by unnatural amino acids.

22 Three N-Fmoc unnatural amino acids (1-3) that contain varying linkers

23 es of mutants site-specific labeled with the unnatural amino acid, [(13)C]p-methoxyphenylalanine, in

24 ne WALP24 peptide labeled with the semirigid unnatural amino acid 4-(3,3,5,5-tetra-methyl-2,6-dioxo-4

25 Two stabilized derivatives incorporating unnatural amino acids ((68)Ga-SH01078 and (68)Ga-P03034)

26 polymerase variant containing a fluorescent unnatural amino acid, (7-hydroxy-4-coumarin-yl) ethylgly

27 ease the utility of protein mutagenesis with unnatural amino acids, a recombinant expression system i

28 Translations with unnatural amino acids (AAs) are generally inefficient, a

29 noacyl-tRNA synthetase pair specific for the unnatural amino acid added to the media and the protein

30 with the ease of synthesis of the necessary unnatural amino acids allows for facile synthesis of tri

31 er-peptide elements with genetically encoded unnatural amino acids (amino acids that have been artifi

32 The synthesis of a third class of unnatural amino acids, amino tetrazolyl alanines ((ATz)A

33 ethodology to incorporate a photoactivatable unnatural amino acid and photochemically cleave the back

34 vancing existing protocols, we introduced an unnatural amino acid and subsequently mineral-interactin

35 combining mRNA display with incorporation of unnatural amino acids and "click" chemistry.

36 yl-tRNA synthetase-tRNA pairs that recognize unnatural amino acids and decode the new codons.

37 fted toward site-specific modification using unnatural amino acids and engineered site-selective amin

38 n reaction was applied to the preparation of unnatural amino acids and geometrically controlled tri-

39 plants or synthetic chemistry can introduce unnatural amino acids and non-peptidic constraints that

40 Incorporation of unnatural amino acids and peptidomimetic residues into t

41 e optimized this system for use with several unnatural amino acids and provide a demonstration of its

42 parameter and topology files to accommodate unnatural amino acids and the delta amide linkages.

43 plied to the diastereoselective synthesis of unnatural amino acids and the late-stage derivatization

44 allows for straightforward incorporation of unnatural amino acids and the preparation of peptides ma

45 on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state

46 Methods for installing natural and unnatural amino acids and their modifications into prote

47 thyl-14- O-methylmorphinans with natural and unnatural amino acids and three dipeptides at position 6

48 ing novel self-associating peptides based on unnatural amino acids, and inhibitor peptides of detrime

49 the amino acid sequence, the introduction of unnatural amino acids, and labeling with stable isotopes

50 oto-cross-linking studies with site-specific unnatural amino acids, and species-specific activity of

51 The unnatural amino acid appears to disrupt the interactions

52 Among them, silicon-containing unnatural amino acids are becoming an interesting new cl

53 specifically incorporating multiple distinct unnatural amino acids are hampered by their low efficien

54 bility and unique conformational properties, unnatural amino acids are highly valued by pharmaceutica

55 In engineered systems, unnatural amino acids are incorporated into proteins to

56 Unnatural amino acids are introduced site-specifically a

57 Unnatural amino acids are key building blocks in therape

58 CR1 (1-350) containing Mn(2+) chelated to an unnatural amino acid assists in the characterization of

59 efficiency incorporation of a single type of unnatural amino acid at a time, because every triplet co

60 created a neopeptide via introduction of an unnatural amino acid at the K(12) position, which could

61 eases the yield of protein, incorporating an unnatural amino acid at three sites, from unmeasurably l

62 F1, we increase the yield of protein bearing unnatural amino acids at a single site 17- to 20-fold.

63 A set of variants containing natural and unnatural amino acids at position 15, which were designe

64 , we used reconstituted 26S proteasomes with unnatural amino-acid-attached fluorophores in a series o

65 l metabolic placement of a uniquely reactive unnatural amino acid, azidohomoalanine (Aha), followed b

66 We have developed a new unnatural amino acid based on the solvatochromic fluorop

67 The ability to genetically encode unnatural amino acids beyond the common 20 has allowed u

68 ry is applied to the gram-scale syntheses of unnatural amino acids, bioactive molecules, and chiral b

69 roperties allow them to incorporate multiple unnatural amino acids (but not natural amino acids), wou

70 ns that are involved in the synthesis of the unnatural amino acid by genetic analysis.

71 r demonstrate that IQF substrates containing unnatural amino acids can be used to investigate proteas

72 his study demonstrates that incorporation of unnatural amino acids can provide a flexible, straightfo

73 128, with a range of alternative natural and unnatural amino acids, changed the EC50 (from approximat

74 it will be possible to encode more than 200 unnatural amino acid combinations using this approach.

75 improved second-generation synthesis of the unnatural amino acid components of the anticancer peptai

76 s enabled a significant increase in yield of unnatural amino acid containing proteins from tens of mi

77 i-Miyaura coupling of genetically positioned unnatural amino acids containing aryl halide side chains

78 RNAs, semi-synthetic organism creation, and unnatural-amino-acid-containing protein synthesis.

79 Genetically encoded unnatural amino acids could facilitate the design of pro

80 -linking coupled with mass spectrometry, and unnatural amino acid cross-linking.

81 2 substrate library, which also included the unnatural amino acid cyclohexylalanine (Cha) derivative

82 d glycine in the selectivity filter with the unnatural amino acid d-Alanine.

83 metathesis as key steps to assemble various unnatural amino acid derivatives and peptides.

84 n of 3-, 4-, 5-, and 6-membered heterocyclic unnatural amino acid derivatives by exploiting facile Ma

85 sters, which may be regarded as novel hybrid unnatural amino acids, during the peptide synthesis itse

86 g selective modification of both natural and unnatural amino acids--each with merits and limitations-

87 We used mutations with natural and unnatural amino acids, electrophysiological recordings,

88 estrict the incorporation of the fluorescent unnatural amino acid epsilonNH2-Bodipy576/589-lysine (BO

89 apoA-I specifically designed to include the unnatural amino acid exclusively at position 166.

90 Moreover, unnatural amino acid expression in the methylotrophic ho

91 nt immunosensor designed by incorporating an unnatural amino acid fluorophore into the binding site o

92 h residues were substituted with natural and unnatural amino acids, focusing on the role of aromatici

93 strong polarity and high hydrophobicity, the unnatural amino acid forms specific and strong interacti

94 sical properties of three of the fluorescent unnatural amino acids from two classes were also studied

95 ledge, successful detection of a fluorescent unnatural amino acid (fUAA), Lys(BODIPYFL), incorporated

96 elanogaster) and the incorporation of useful unnatural amino acids has been aided by the development

97 Using this system multiple distinct unnatural amino acids have been incorporated and used to

98 ty filter region of the NaK channel with the unnatural amino acids homoserine and cysteine sulfonic a

99 As well, using a UV-crosslinking unnatural amino acid in KCNE1, we found EQQQQ and EQQ cr

100 ssible to genetically encode a wide array of unnatural amino acids in both prokaryotic and eukaryotic

101 highlight the utility of genetically encoded unnatural amino acids in exploring the effects of posttr

102 The incorporation of both unnatural amino acids in place of a phenylalanine in the

103 ally-encoded, site-specific incorporation of unnatural amino acids in regions essential for activatio

104 in P. pastoris and used to incorporate eight unnatural amino acids in response to an amber codon with

105 e to efficiently direct the incorporation of unnatural amino acids in response to quadruplet codons.

106 have been evolved to incorporate a range of unnatural amino acids in response to the amber codon in

107 o pEVOL and compared their ability to insert unnatural amino acids in response to three independent a

108 o-Q1 we direct the incorporation of distinct unnatural amino acids in response to two of the new blan

109 rrounding residues but have not incorporated unnatural amino acids in this process.

110 nd enables the preparation of Fmoc-protected unnatural amino acids in three steps.

111 Receptors rescued by incorporation of unnatural amino acids, including p-benzoyl-l-phenylalani

112 With this new system, a number of unnatural amino acids, including the photocrosslinkers p

113 arnessing proximity-enabled reactivity of an unnatural amino acid incorporated in the bait toward a t

114 Here, using unnatural amino acid incorporation and fluorine-19 nucle

115 ent advances are enhancing the efficiency of unnatural amino acid incorporation by creating and evolv

116 nstrate that tRNA levels can be limiting for unnatural amino acid incorporation efficiency, and we de

117 ns and highlight the exceptional capacity of unnatural amino acid incorporation for increasing our un

118 don suppression and related technologies for unnatural amino acid incorporation has greatly expanded

119 n O-ribosome previously evolved for enhanced unnatural amino acid incorporation in response to amber

120 The efficiency of unnatural amino acid incorporation in response to the am

121 codons, to provide a substantial increase in unnatural amino acid incorporation in response to the UA

122 Unnatural amino acid incorporation into ion channels has

123 Improving the yield of unnatural amino acid incorporation is an important chall

124 ress in this area: (i) the low efficiency of unnatural amino acid incorporation that limits labeling

125 of the nonsense suppression methodology for unnatural amino acid incorporation to probe drug-recepto

126 enesis and the high precision methodology of unnatural amino acid incorporation to study the gating i

127 nstituted in vitro translation, quantitative unnatural amino acid incorporation via AUG codon reassig

128 heterogeneity in GCK using a combination of unnatural amino acid incorporation, time-resolved fluore

129 Using unnatural amino acid incorporation, we demonstrate the r

130 transfer RNA (tRNA) synthetase/tRNA pair for unnatural amino acid incorporation.

131 m Methanococcus janaschii Tyr synthetase for unnatural amino acid incorporation.

132 nto the factors that control the fidelity of unnatural amino acid incorporation.

133 ite-specific incorporation of an immunogenic unnatural amino acid into a protein of interest produces

134 ite-specific incorporation of an immunogenic unnatural amino acid into an autologous protein offers a

135 pair enabling site-specific insertion of the unnatural amino acid into apoA-I.

136 ciently and site-specifically incorporate an unnatural amino acid into proteins.

137 In the present work, we incorporated an unnatural amino acid into the alpha-hemolysin (alphaHL)

138 d by the introduction of an azide-containing unnatural amino acid into the coat protein for the first

139 er RNAs to encode numerous pairs of distinct unnatural amino acids into a single protein expressed in

140 n vivo incorporation of isotopically labeled unnatural amino acids into large proteins drastically re

141 However, the efficient incorporation of unnatural amino acids into proteins and the specific, fl

142 h mutually orthogonal methods of introducing unnatural amino acids into proteins as well as with chem

143 new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli

144 nse, rare, or 4-bp codons to incorporate >50 unnatural amino acids into proteins in Escherichia coli,

145 The efficient, site-specific introduction of unnatural amino acids into proteins in mammalian cells i

146 that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells.

147 erein we review work on the incorporation of unnatural amino acids into proteins in response to quadr

148 that specifically prevents incorporation of unnatural amino acids into proteins may provide a new st

149 n for the incorporation of multiple distinct unnatural amino acids into proteins or the synthesis and

150 Site-specific incorporation of unnatural amino acids into proteins provides a powerful

151 To encode efficiently many distinct unnatural amino acids into proteins we require blank cod

152 sion, for the site-specific incorporation of unnatural amino acids into proteins, is currently limite

153 principles and methods for incorporation of unnatural amino acids into proteins.

154 een used to enable the incorporation of many unnatural amino acids into recombinant proteins in vivo.

155 bles efficient, homogeneous incorporation of unnatural amino acids into target proteins in diverse ma

156 e basis for ion selectivity by incorporating unnatural amino acids into the channel, engineering chan

157 prove useful for the incorporation of bulky, unnatural amino acids into the genetic code.

158 e ethynyl group of pENPhe suggests that this unnatural amino acid is a more conservative substitution

159 Escherichia coli MS methods verify that the unnatural amino acid is only incorporated at one positio

160 nt synthesis of products containing multiple unnatural amino acids is challenging.

161 urements, because efficient incorporation of unnatural amino acids is limited to transient expression

162 ural changes, since the incorporation of the unnatural amino acids is not inherently limited by the s

163 anted to report the utility of an unexplored unnatural amino acid, isothiocyanyl alanine ((NCS)Ala =

164 ed have been evolved to incorporate numerous unnatural amino acids, it will be possible to encode mor

165 or labeling through genetic incorporation of unnatural amino acids, lanthanide resonance energy trans

166 This paper introduces the unnatural amino acids m-Abc(2K) and o-Abc(2K) as nanomet

167 ension of approaches to incorporate multiple unnatural amino acids may allow the combinatorial biosyn

168 Using next-generation sequencing and unnatural amino acid-mediated protein-DNA cross-linking,

169 Specifically, we create the pY protein by unnatural amino acid misincorporation, mutagenize a univ

170 This method uses an unnatural amino acid-modified Src homology 2 (SH2) domai

171 We report a general unnatural amino acid mutagenesis approach to quantify th

172 ge-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.

173 Previous studies have established that unnatural amino acid mutagenesis can probe three key bin

174 Here we use unnatural amino acid mutagenesis coupled with agonist an

175 nto AMPA receptors using genetically encoded unnatural amino acid mutagenesis in a mammalian cell lin

176 hotocaging of a synthetic gene network using unnatural amino acid mutagenesis in mammalian cells was

177 Further, unnatural amino acid mutagenesis is used to replace the

178 recision structural modifications enabled by unnatural amino acid mutagenesis on mammalian receptors

179 The advent of unnatural amino acid mutagenesis opens up a new toolbox

180 Here we use unnatural amino acid mutagenesis to characterize binding

181 Here we use unnatural amino acid mutagenesis to examine the ligand b

182 here we use double mutant cycle analysis and unnatural amino acid mutagenesis to probe the functional

183 oteins expressed in Escherichia coli through unnatural amino acid mutagenesis using a Cnf-specific tR

184 tives into the receptor binding pocket using unnatural amino acid mutagenesis, and evaluating the res

185 ompared with previously reported vectors for unnatural amino acid mutagenesis.

186 Using unnatural amino-acid mutagenesis, we subtly altered the

187 ereoselective route to a masked form of this unnatural amino acid now enabled the synthesis of two of

188 to this problem, we genetically encoded the unnatural amino acids o-nitrobenzyl-2-fluorotyrosine, -3

189 In this method, the genetically encoded unnatural amino acid p-acetyl-L-phenylalanine (p-AcPhe)

190 For these studies, we have incorporated the unnatural amino acid p-acetyl-L-phenylalanine for specif

191 The unnatural amino acid p-acetylphenylalanine (pAcF) was si

192 tive way to modify protein N-termini and the unnatural amino acid p-aminophenylalanine (paF).

193 First, we genetically encoded the unnatural amino acid p-azido-L-phenylalanine (azF) at va

194 By replacing LptD residues with an unnatural amino acid p-benzoyl-L-phenyalanine (pBPA) and

195 n suppression to introduce the photoreactive unnatural amino acid p-benzoyl-l-phenylalanine (BzF) at

196 By site-specific incorporation of the unnatural amino acid p-benzoyl-L-phenylalanine, a photor

197 incorporation of the UV photo-cross-linkable unnatural amino acid p-benzoyl-L-phenylalanine.

198 The unnatural amino acid p-Benzoylphenylalanine was successf

199 Here we demonstrate that the unnatural amino acid p-boronophenylalanine (BF) confers

200 The site-specific incorporation of the unnatural amino acid p-nitrophenylalanine (pNO(2)Phe) in

201 We have developed a method to incorporate an unnatural amino acid, p-acetylphenylalanine (pAcPhe) int

202 ated to a mutant alphaCD3 Fab containing the unnatural amino acid, p-acetylphenylalanine, at a define

203 CLL1-alphaCD3, using the genetically encoded unnatural amino acid, p-acetylphenylalanine.

204 C identical withN stretching vibration of an unnatural amino acid, p-cyano-phenylalanine, to directly

205 movement by incorporating tryptophan and the unnatural amino acid, p-cyanophenylalanine into Glt(Ph)

206 The unnatural amino acid para-azido-L-phenylalanine was inco

207 Here, we have used unnatural amino acid photo-cross-linking to investigate

208 By incorporating unnatural amino acid photochemical and new click-chemica

209 Unnatural amino acids play an important role in peptide

210 to increase the structural diversity of the unnatural amino acid pool is needed.

211 h nanomaterials by targeted incorporation of unnatural amino acids possessing dual affinity to differ

212 es rely mainly on traditional coupling using unnatural amino acids, postsynthetic modification of pep

213 Thus, an encoded unnatural amino acid produces a chemical "handle" by whi

214 The methodology establishes novel routes to unnatural amino acids, proline homologues, and cyclic vi

215 not be feasible to separate the full-length unnatural amino acid protein from the truncated form.

216 Genetically encoded unnatural amino acids provide powerful strategies for mo

217 mination of a chiral center derived from the unnatural amino acid R-alpha-methylcysteine makes the mo

218 gth overlap and highly selective natural and unnatural amino acid recognition sequences.

219 e first experimental evidence documenting an unnatural amino acid replacement in a GPCR expressed in

220 tural amino acids will extend the use of the unnatural amino acid replacement methodology to amino ac

221 o acids are transformed to twenty-one chiral unnatural amino acids representing seven distinct functi

222 e site specifically inserted a (13)C-labeled unnatural amino acid residue, (13)C-p-methoxyphenylalani

223 Many important and useful natural and unnatural amino acid salts can be produced in excellent

224 This approach provides hybrid natural and unnatural amino acid sequences, and thus we termed it th

225 llows selection to yield proteins containing unnatural amino acids should such sequences functionally

226 This Perspective highlights the diversity of unnatural amino acid structures found in hit-to-lead and

227 Using unnatural amino acid substitution and a standard linkage

228 sized a library of 61 individual natural and unnatural amino acids substrates, chosen to cover a broa

229 achieved using peptide libraries containing unnatural amino acids such as the hybrid combinatorial s

230 ombination of self-labeling protein tags and unnatural amino acid technology permits the semisynthesi

231 Unnatural amino acid technology provides a degree of bio

232 he binding site of the binding protein using unnatural amino acid technology.

233 An unnatural amino acid termed a proline-templated glutamic

234 O-tert-Butyltyrosine (Tby) is an unnatural amino acid that can be site-specifically incor

235 Bpa is a photoaffinity label unnatural amino acid that can form covalent bonds with a

236 Specifically, we report an unnatural amino acid that preferentially labels amine-pr

237 otein an appropriately "tuned" electrophilic unnatural amino acid that reacts with a native nucleophi

238 eted cellular proteins with versions bearing unnatural amino acids that allow imaging or synthetic re

239 bacteria, limiting the types and numbers of unnatural amino acids that can be utilized at any one ti

240 lational, site-specific incorporation of two unnatural amino acids that can function as fluorescence

241 We show, using a photoreactive, unnatural amino acid, that Asf1 tail residue 210 cross-l

242 Here, we incorporate an unnatural amino acid, thia-methylimidazole, at this site

243 Ita), for the synthesis of another class of unnatural amino acids, thioureayl alanines ((TU)Ala = Tu

244 The expression of proteins containing unnatural amino acids through suppression of a stop codo

245 addition, SIDEpro can accommodate any PTM or unnatural amino acid, thus providing a flexible predicti

246 that binds to the hot spot, and selected an unnatural amino acid to incorporate, based on the struct

247 f covalent bond into proteins by enabling an unnatural amino acid to react with a proximal cysteine.

248 redoxins, and demonstrate the power of using unnatural amino acids to address critical chemical biolo

249 of His-66 may improve the ability of certain unnatural amino acids to be incorporated by the ribosome

250 tantially enhanced this by incorporating 102 unnatural amino acids to explore the S1-S4 pockets of hu

251 In this study, we used a set of unnatural amino acids to fully map the substrate prefere

252 This method entails the incorporation of unnatural amino acids to site-specifically label protein

253 ta suggest not only robust methods for using unnatural amino acids to study large proteins by NMR but

254 The stereoselective synthesis of the unnatural amino acid tubuvaline is accomplished using te

255 Using only between 8 and 25 mg of unnatural amino acid, typically 2 mg of FAS-TE, sufficie

256 In our system, any genetically encoded unnatural amino acid (UAA) can be used as a small-molecu

257 aviolet irradiation of a photo-crosslinkable unnatural amino acid (UAA) cotranslationally incorporate

258 monstrate the application of a photoreactive unnatural amino acid (UAA) crosslinking system to captur

259 Through multi-site unnatural amino acid (UAA) incorporation, these protein

260 tached to proteins within living cells using unnatural amino acid (UAA) mutagenesis.

261 echnology to site-specifically introduce the unnatural amino acid (UAA) p-azido-l-phenylalanine (azF)

262 (AMPA)-type iGluRs using genetically encoded unnatural amino acid (UAA) photocross-linkers, p-benzoyl

263 Genetically incorporated unnatural amino acid (UAA) technologies are powerful too

264 ally introduced into proteins by enabling an unnatural amino acid (Uaa) to selectively react with a p

265 ptor by genetically encoding a photoreactive unnatural amino acid (UAA).

266 essential HIV-1 protein biosynthesis through unnatural amino acid (UAA*)-mediated suppression of geno

267 r optical control of neuronal proteins using unnatural amino acids (Uaa) in vivo is lacking.

268 The ability to incorporate unnatural amino acids (UAA) into proteins in a site spec

269 nsporter protein to encode photocrosslinking unnatural amino acids (UAAs) into 75 different positions

270 The site-specific incorporation of unnatural amino acids (UAAs) into proteins in living cel

271 The ability to site-specifically incorporate unnatural amino acids (UAAs) into proteins is a powerful

272 rm is reported that enables incorporation of unnatural amino acids (UAAs) into specific sites on the

273 click chemistry and the genetic encoding of unnatural amino acids (UAAs) to overcome this limitation

274 has led to the addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of Esc

275 Here, we show that unnatural amino acids (UAAs) with orthogonal chemical re

276 Fluorescent unnatural amino acids (UAAs), when genetically incorpora

277 onal aaRS to distinguish between a favorable unnatural amino acid (unAA) substrate from unfavorable n

278 hydrate-binding proteins that use a reactive unnatural amino acid "warhead" and demonstrates that a "

279 ogonal tRNA-synthetase pair, the fluorescent unnatural amino acid was incorporated in the Shaker volt

280 By imaging with fluorescent unnatural amino acid, we found that menthol binding indu

281 s-linking experiments with photo-activatable unnatural amino acids, we show that full-length BACE1, i

282 Saccharomyces cerevisiae to be specific for unnatural amino acids were inserted between eukaryotic t

283 Unnatural amino acids were introduced by chemical synthe

284 the catalytic cycle can be maintained using unnatural amino acids, which may prove useful in enzyme

285 f high yields of complex proteins containing unnatural amino acids whose expression is not practical

286 application of a peptide delivery system for unnatural amino acids will extend the use of the unnatur

287 arget protein contains a genetically encoded unnatural amino acid with bioorthogonal reactivity and t

288 ara-pentafluorosulfanyl phenylalanine, is an unnatural amino acid with extreme physicochemical proper

289 antibodies containing a genetically encoded unnatural amino acid with orthogonal chemical reactivity

290 We genetically encoded four unnatural amino acids with a diverse set of functional g

291 mized system, we produce proteins containing unnatural amino acids with comparable yields to a protei

292 e access to two conformationally constrained unnatural amino acids with different dispositions of the

293 y relationship for binding using a series of unnatural amino acids with different lengths of hydropho

294 eral strategy for labelling pairs of encoded unnatural amino acids with different probes via rapid an

295 labeling of proteins at genetically encoded unnatural amino acids with distinct small molecules at p

296 Using this new methodology, we incorporated unnatural amino acids with extended side chains into the

297 site-specific incorporation of a variety of unnatural amino acids with novel chemical and biological

298 e demonstrate the use of genetically encoded unnatural amino acids with orthogonal chemical reactivit

299 s were constructed using genetically encoded unnatural amino acids with orthogonal chemical reactivit

300 The synthesis of unnatural amino acids with small side-chain functionalit