ライフサイエンスコーパス: unnatural base pair

1 rs two additional letters that form a third, unnatural base pair.

2 the effort to develop stable and replicable unnatural base pairs.

3 polymerase-mediated replication of the same unnatural base pairs.

4 a large number of predominantly hydrophobic unnatural base pairs.

5 on of natural base pairs also apply to AEGIS unnatural base pairs.

6 At this point, the unnatural base pair adopts a Watson-Crick geometry, and

7 These marker nucleotides constitute an unnatural base pair, allowing large quantities of marked

8 ch should aid in further optimization of the unnatural base pair and possibly in the design of additi

9 e of extension of primers terminating at the unnatural base pair and, interestingly, requires that th

10 ase I to synthesize and extend the different unnatural base pairs and mispairs under steady-state con

11 ation properties of many previously examined unnatural base pairs and should help design unnatural ba

12 nus that seem to limit replication of larger unnatural base pairs, and they therefore represent a pro

13 han dMMO2 and that overall the corresponding unnatural base pairs are generally replicated with highe

14 Several unnatural base pairs are identified that are both reason

15 The differences between these unnatural base pairs are manifest only in the polymerase

16 Surprisingly, several of the unnatural base pairs are virtually as stable as a natura

17 terize a library of templates containing the unnatural base pair as a function of amplification.

18 The most promising unnatural base pair candidate of this series is the 3-fl

19 We show here that both of the unnatural base pairs d5SICS:dMMO2 and d5SICS:dNaM are se

20 s them to replicate a plasmid containing the unnatural base pair dNaM-dTPT3.

21 The unnatural base pairs do not appear to induce major struc

22 a wide variety of predominantly hydrophobic unnatural base pairs exemplified by d5SICS-dMMO2 and d5S

23 netic alphabet, we have developed a class of unnatural base pairs, exemplified by d5SICS-dMMO2 and d5

24 We recently developed a class of candidate unnatural base pairs, exemplified by the pair formed bet

25 ts significant progress toward developing an unnatural base pair for the in vivo expansion of an orga

26 onents provides the basis to further develop unnatural base-pairs for synthetic biology applications.

27 timization that led to identification of the unnatural base pair formed between the nucleotides dMMO2

28 Previously, the unnatural base pairs formed between d5SICS and either dN

29 orted the synthesis and thermal stability of unnatural base pairs formed between nucleotides bearing

30 We now report the synthesis and stability of unnatural base pairs formed between simple phenyl rings

31 its expansion by the development of a third, unnatural base pair has emerged as a central goal of che

32 A variety of unnatural base pairs have been shown to be formed effici

33 While a variety of unnatural base pairs have been shown to be substrates fo

34 ntaining 2'-O-methyl-modified nucleotides or unnatural base pairs, have been evolved.

35 aM is one of the most efficiently replicated unnatural base pairs identified to date, but its pairing

36 ino acid, and efficient participation of the unnatural base pair in decoding at the ribosome.

37 ted that this method can be used to discover unnatural base pairs in DNA with a detection threshold o

38 mation requires in vivo transcription of the unnatural base pair into mRNA and tRNA, aminoacylation o

39 We found that the unnatural base pair is efficiently replicated with high

40 mistry complexes in which the already formed unnatural base pair is positioned at the postinsertion s

41 This unnatural base pair is replicated by the Klenow fragment

42 Moreover, polymerase recognition of the unnatural base pairs is found to be very sensitive to bo

43 zed, the detailed recognition of most of the unnatural base pairs is generally polymerase dependent.

44 ell as continued primer extension beyond the unnatural base pair, is sensitive to the specific methyl

45 ining either the d5SICS-dNaM or d5SICS-dMMO2 unnatural base pair may be amplified by PCR with fidelit

46 strate that the determinants of a functional unnatural base pair may be designed into predominantly h

47 Importantly, the results reveal that the unnatural base pairs may function within an expanded gen

48 pand the genetic alphabet, by addition of an unnatural base pair, promise to expand the biotechnologi

49 Not only are these unnatural base pairs promising for different application

50 While we find that some aspects of unnatural base pair recognition are conserved among the

51 a allow us to propose a generalized model of unnatural base pair replication, which should aid in fur

52 ncing of an epigenetic base by exploiting an unnatural base pair system.

53 unnatural base pairs and should help design unnatural base pairs that are better replicated.

54 Through the development of unnatural base pairs that are compatible with native DNA

55 air and possibly in the design of additional unnatural base pairs that are replicated with truly natu

56 As part of an effort to develop unnatural base pairs that are stable and replicable in D

57 ply that further modifications may result in unnatural base pairs that can be both efficiently synthe

58 imize the slowest step of replication of the unnatural base pair, the insertion of dMMO2 opposite d5S

59 es the pairing nucleotides of the developing unnatural base pair to adopt a planar Watson-Crick-like

60 We exemplify use of this unnatural base pair to sequence 5-formylcytosine in a DN

61 We have developed an unnatural base pair (UBP) and a semisynthetic organism (

62 two synthetic nucleotides that form a third, unnatural base pair (UBP) have recently yielded three pr

63 semisynthetic organism (SSO) that retains an unnatural base pair (UBP) in its DNA, transcribes it int

64 f unnatural nucleotides that pair to form an unnatural base pair (UBP).

65 increased information, we have developed the unnatural base pairs (UBPs) dNaM and d5SICS or dTPT3 (dN

66 A molecules with synthetic modifications and unnatural base pairs (UBPs) for a variety of biotechnica

67 The development of unnatural base pairs (UBPs) has greatly increased the in

68 Unnatural base pairs (UBPs) have been developed and used

69 Os) that by virtue of a family of synthetic, unnatural base pairs (UBPs), store and retrieve increase

70 ineering to identify variants that replicate unnatural base pairs (UBPs), unnatural backbones, tags,

71 We have developed a family of unnatural base pairs (UBPs), which rely on hydrophobic a

72 lphabet has been expanded to include several unnatural base pairs (UBPs).

73 the creation of artificial extra base pairs (unnatural base pairs, UBPs) are opening the door to a ne

74 The specificity of this unnatural base pair was studied by biophysical DNA therm

75 f an effort to develop stable and replicable unnatural base pairs, we have evaluated a large number o

76 We synthesized a panel of unnatural base pairs whose pairing depends on hydrophobi

77 base pairs, and the development of a third, unnatural base pair would increase the genetic and chemi